R3202 Product Catalogue FF 2013 EN Low Res
User Manual: R3202
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R-Biopharm AG Product catalogue Food & Feed Analysis R-Biopharm – for the safety of your analysis. 2017 Product catalogue 2017 Food & Feed Analysis R-Biopharm AG – Product catalogue 2017 Content Overview of test systems by R-Biopharm Enzymatic analysis • • • • • • "Yellow Line" Roche Diagnostics Enzytec™ Color Enzytec™ Fluid Enzytec™ Generic Enzytec™ Liquid RIDA®CUBE (only for RIDA®CUBE SCAN*) Vitamin analysis 6 8 10 11 11 12 12 13 • VitaFast® • EASI-EXTRACT® 14 16 17 • RIDASCREEN® 17 Mycotoxins 18 18 26 30 33 • • • • ELISA, lateral flow and immunoaffinity columns Automated online analysis Standards for mycotoxin analysis Reference material for mycotoxin analysis Residues • • • • Hormones & anabolics Antibiotics Phycotoxins Food adulteration 34 34 38 43 43 Histamine 43 Allergens 44 44 • ELISA, lateral flow and real-time PCR 4 GMO • • • • DNA preparation Screening Real-time PCR – qualitative DNA detection Real-time PCR – quantitative DNA detection Identification of animal species/risk material/BSE • • • • • Real-time PCR – qualitative DNA detection Real-time PCR – quantitative DNA detection SureFood® FISH ID Risk material BSE 54 56 56 56 57 58 60 61 62 63 63 Microbiology/hygiene 64 • Culture medium systems for colony counting and pathogen detection in food or surface samples • Culture medium systems for surface specimen/hygiene monitoring • Pathogens & bacterial toxins • Viruses • Water analysis • Beverage analysis • Test Systems for cleaning control 66 67 68 70 70 71 73 Equipment/software/accessories • Equipment • Software • Accessories 74 76 77 78 Explanation 79 The R-Biopharm Group – contact us 80 General Terms & Conditions of R-Biopharm AG 82 5 R-Biopharm AG – Product catalogue 2017 Overview of test systems by R-Biopharm ELISA – RIDASCREEN® • • • • Quantitative results Applications for many matrices Analysis by RIDA®SOFT Win Can be automated LFD – RIDA®QUICK • Immunochromatographic tests • Applications for many matrices • Visual evaluation (qualitative and semi-quantitative) • Qualitative evaluation (analysis by RIDA®QUICK SCAN and RIDA®SMART APP) Immunoaffinity columns – PREP®, EASI-EXTRACT® • For sample preparation prior to analysis by HPLC, LC-MS/MS or ELISA • Single and multiparameter • High specificity • For simple and complex matrices Enzymatic analytics – Roche, Enzytec™, RIDA®CUBE • UV-tests (reference methods) • Tests for automation • Single-use cartridge system 6 Real-time PCR – SureFood®/SureFast® • • • • Modular, open test systems DNA/RNA preparation, screening, identification, quantification Single and multiplex tests Suitable for all established thermal cyclers Quality assurance – Trilogy® • • • • Standard materials for calibration (crystalline & liquid) Reference materials (naturally contaminated matrices) Materials for quality control RIDA® spiking solutions for validation Ready to use culture medium systems – RIDA®STAMP, Compact Dry • As proof of bacteria, yeasts and moulds • For food samples and surface analysis • Chromogenic proof systems for simple identification and enumeration of colonies Equipment/automation • Small automates for on site single testing • Automates for 1 - 2 microtiter plates • Evaluation with RIDA®SOFT Win 7 R-Biopharm AG – Product catalogue 2017 Enzymatic analysis for food and feed Enzymatic tests are widely used as analytical tools for the analysis of food products such as fruit juices, wine or beer, dairy products, eggs and meat. Enzymatic test kits determine sugars, acids, alcohols and a few other food components. They are based on high quality enzymes, enabling precise and specific measurements of each compound, even in complex matrices. Results are measured with a spectrophotometer and automation is possible. Numerous enzymatic methods have been approved or validated by international organisations. The “Yellow line” kits are produced by Roche (previously Boehringer Mannheim), with more than 40 years of experience in the production of the enzymes, which are the key element of each test. The Roche test kits have been used and validated worldwide for several decades, with many corresponding publications. They have been selected as reference method by many international organizations and they are still the reference quality today. 8 As an alternative, R-Biopharm also offers the Enzytec™ Generic line. The Enzytec™ Color line is a new product range for colorimetric assays. They are based on a chemical reaction with a chromogen in the visible range, without using any enzyme. Enzytec™ Fluid kits are produced by Thermo Scientific, whereas Enzytec™ Liquid kits are produced by R-Biopharm. These reagents are all liquid and ready-to-use, so they can be placed directly on any biochemistry analyser and stay on board for true random-access capability. The new product line RIDA®CUBE enables single testing. The test cartridges are ready-to-use and allow a rapid analysis. The RIDA®CUBE kits can only be used in combination with the RIDA®CUBE SCAN instrument. Roche "Yellow Line" • Reference quality for more than 40 years • 31 tests for all requirements in the food industry • Produced by Roche Diagnostics Enzytec™ Fluid or Enzytec™ Liquid • Liquid, ready-to-use reagents • Stable until end of shelf-life, even after opening • Easy and safe use on biochemistry analysers RIDA®CUBE SCAN • Small but precise like a big biochemistry analyser • Ready-to-use test cartridges for single testing • Only one pipeting step and the result after 15 minutes. 9 R-Biopharm AG – Product catalogue 2017 Enzymatic food analysis "Yellow Line" Roche Diagnostics Product Description "Yellow Line" Roche Diagnostics Acids Acetic acid Enzymatic test (340 nm) 3 x 11 determinations 10148261035 L-Ascorbic acid Enzymatic test (578 nm) 21 determinations 10409677035 Citric acid Enzymatic test (340 nm) 3 x 12 determinations 10139076035 Formic acid Enzymatic test (340 nm) 21 determinations 10979732035 D-Gluconic acid Enzymatic test (340 nm) 27 determinations 10428191035 L-Glutamic acid Enzymatic test (492 nm) 3 x 13 determinations 10139092035 D-3-Hydroxybutyric acid Enzymatic test (492 nm) 3 x 12 determinations 10907979035 D-Isocitric acid Enzymatic test (340 nm) 33 determinations 10414433035 D-/L-Lactic acid Enzymatic test (340 nm) 30 determinations of each 11112821035 L-Lactic acid Enzymatic test (340 nm) 30 determinations 10139084035 D-Malic acid Enzymatic test (340 nm) 3 x 11 determinations 11215558035 L-Malic acid Enzymatic test (340 nm) 30 determinations 10139068035 Succinic acid Enzymatic test (340 nm) 11 determinations 10176281035 "Yellow Line" Roche Diagnostics Sugars D-Glucose Enzymatic test (340 nm) 3 x 45 determinations 10716251035 D-Glucose/D-Fructose Enzymatic test (340 nm) 27 determinations of each 10139106035 Lactose/D-Galactose Enzymatic test (340 nm) 32 determinations 10176303035 Lactose/D-Glucose Enzymatic test (340 nm) 32 determinations of each 10986119035 Maltose/Sucrose/D-Glucose Enzymatic test (340 nm) 15 determinations of each 11113950035 Raffinose Enzymatic test (340 nm) 32 determinations 10428167035 Sucrose/D-Glucose Enzymatic test (340 nm) 22 determinations of each 10139041035 Sucrose/D-Glucose/D-Fructose Enzymatic test (340 nm) 22 determinations of each 10716260035 Starch Enzymatic test (340 nm) 27 determinations 10207748035 "Yellow Line" Roche Diagnostics Others Acetaldehyde Enzymatic test (340 nm) 3 x 11 determinations 10668613035 Ammonia Enzymatic test (340 nm) 50 determinations 11112732035 Urea/Ammonia Enzymatic test (340 nm) 25 determinations of each 10542946035 Cholesterol Enzymatic test (405 nm) 31 determinations 10139050035 Ethanol Enzymatic test (340 nm) 33 determinations 10176290035 Glycerol Enzymatic test (340 nm) 3 x 11 determinations 10148270035 Nitrate Enzymatic test (340 nm) 3 x 13 determinations 10905658035 D-Sorbitol/Xylitol Enzymatic test (492 nm) 3 x 12 determinations 10670057035 Sulfite (SO2) Enzymatic test (340 nm) 31 determinations 10725854035 "Yellow Line" Roche Diagnostics Accessories Cuvettes Holder 1 10019624035 Plastic Spatulas 500 pcs. 10019623035 10 No. of tests/amount Art. No. Enzymatic food analysis Enzytec™ Color Product Description Enzytec™ Color Test kits No. of tests/amount Art. No. Copper Colorimeric test (580 nm) 2 x 50 ml E2400 Iron Colorimeric test (580 nm) 4 x 100 ml E2300 GlucaTest®S125 GlucaTest®L500 Colorimeric test (550 nm) 125 ml (40 tests) 4 x 125 ml (160 tests) E3500 E3550 Free sulfite Colorimeric test (340 nm) 2 x 100 ml E3300 Total sulfite Colorimeric test (340 nm) 2 x 100 ml E3200 Tartaric acid Colorimeric test (520 nm) 2 x 80 ml E3100 Enzytec™ Fluid Enzytec™ Fluid Acids Acetic acid Enzymatic test (340 nm) For automation only E5226 D-Lactic acid Enzymatic test (340 nm) 4 x 10 determinations E5240 L-Lactic acid Enzymatic test (340 nm) 4 x 10 determinations E5260 L-Malic acid Enzymatic test (340 nm) 4 x 10 determinations E5280 Enzytec™ Fluid Sugars D-Fructose Enzymatic test (340 nm) 4 x 10 determinations E5120 D-Glucose Enzymatic test (340 nm) 4 x 10 determinations E5140 Glucose/Fructose Enzymatic test (340 nm) 4 x 10 determinations E5160 Sucrose (via Glucose) Enzymatic test (340 nm) 4 x 10 determinations E5180 Enzytec™ Fluid Others Ammonia Enzymatic test (340 nm) 4 x 10 determinations E5390 Ethanol Enzymatic test (340 nm) 4 x 10 determinations E5340 Glycerol Enzymatic test (340 nm) 4 x 10 determinations E5360 Enzytec™ Fluid Standards Alcohol Standard Alcohol assay control solution 10 x 1 ml E5420 Sugar combination Standard Multi-sugar assay control solution 3 x 3 ml E5440 Sugar Standard for automation Multi-sugar calibration solution for automation 3 x 3 ml E5450 11 R-Biopharm AG – Product catalogue 2017 Enzymatic food analysis Enzytec™ Generic Product Description Enzytec™ Generic Acids No. of tests/amount Art. No. Acetic acid Enzymatic test (340 nm) 2 x 16 determinations E1226 L-Ascorbic acid Enzymatic test (578 nm) 3 x 8 determinations E1267 Citric acid Enzymatic test (340 nm) 24 determinations E1214 D-Gluconic acid Enzymatic test (340 nm) 32 determinations E1223 D/L-Lactic acid Enzymatic test (340 nm) 32 determinations E1255 L-Lactic acid Enzymatic test (340 nm) 32 determinations E1254 L-Malic acid Enzymatic test (340 nm) 32 determinations E1215 Oxalic acid Enzymatic test (590 nm) 10 determinations E2100 Sample purifier Sample preparation for Enzymatic tests 20 samples E2250 Enzytec™ Generic Sugars D-Glucose Enzymatic test (340 nm) 32 determinations E1210 D-Glucose/D-Fructose Enzymatic test (340 nm) 32 determinations of each E1245 Lactose/D-Galactose Enzymatic test (340 nm) 32 determinations E1213 Starch Enzymatic test (340 nm) 32 determinations E1268 Sucrose/D-Glucose Enzymatic test (340 nm) 16 determinations of each E1246 Sucrose/D-Glucose/D-Fructose Enzymatic test (340 nm) 16 determinations of each E1247 Glucose remover For removal of glucose excess in samples 32 E3400 Enzytec™ Generic Standards Multi-acid standard manual Multi-acid assay control solution 9 ml E1240 Multi-acid standard for automation Multi-acid calibration solution for automation 9 ml E1241 Sugar Standard manual Multi-sugar assay control solution 9 ml E1242 Enzytec™ Liquid Enzytec™ Liquid Acids Acetic acid Enzymatic test (340 nm) For automation only E8226 D-/L-Lactic acid Enzymatic test (340 nm) 2 x 25 determinations E8240 Lactic acid Enzymatic test (340 nm) 2 x 25 determinations E8260 Malic acid Enzymatic test (340 nm) 2 x 25 determinations E8280 Enzytec™ Liquid Sugars D-Glucose Enzymatic test (340 nm) 2 x 25 determinations E8140 D-Glucose/D-Fructose Enzymatic test (340 nm) 2 x 25 determinations E8160 Sucrose/D-Glucose Enzymatic test (340 nm) 2 x 25 determinations E8180 Sucrose/D-Glucose/D-Fructose Enzymatic test (340 nm) 2 x 25 determinations E8190 Enzytec™ Liquid Others Ethanol Enzymatic test (340 nm) 2 x 25 determinations E8340 12 Enzymatic food analysis RIDA®CUBE (only for RIDA®CUBE SCAN*) Product Description RIDA®CUBE Ready to use cartridges No. of tests/amount Art. No. Acetic acid Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4226 Ethanol Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4340 Glucose Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4140 D-Glucose/D-Fructose Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4160 DL-Lactic (without differenciation) Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4240 L-Lactic acid Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4260 L-Malic acid Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4280 SO2-Free (Free Sulfite) Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4610 SO2-Total (Total Sulfite) Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4600 Sucrose/D-Glucose Enzymatic test only with RIDA®CUBE SCAN (340 nm) 32 determinations RCS4180 * See page 76 – • Equipment/software/accessories. 13 R-Biopharm AG – Product catalogue 2017 Vitamin analysis in food, feed and vitamin containing products Food products are now being enriched and fortified with vitamins in many forms. But does the amount present in the food at the end of the shelf life match the label on the package? Food manufacturers, regulatory agencies and commercial laboratories should therefore have analytical methods on hand that allow them to quickly and reliably determine the natural and added vitamin content of food products. Product testing: There are different methods for analysing water soluble vitamins: ELISA, immunoaffinity columns (IAC), microbiological and enzymatic microtiter plate tests. The new developed RIDASCREEN®FAST Vitamin B12 and Folic Acid tests allow a quantitative determination of both vitamins within 1 h. The total vitamin B12 content is determined without using cyanide. Regarding folic acid and biotin the added vitamin content is determined. 14 When using Immunoaffinity columns in conjunction with HPLC or LC-MS/MS, the sample is purified and the vitamin is retained by the antibody in the column. Using the EASI-EXTRACT® VITAMIN B12 and BIOTIN (IAC), you can determine the total vitamin content. With the EASI-EXTRACT® FOLIC ACID (IAC) you can only determine added folic acid. Depending on the sample preparation the added or total vitamin content can be determined with the microbiological VitaFast® test. With the enzymatic VitaFast® Vitamin C test in microtiter plate format a determination of total vitamin C content (L-ascorbic acid and L-dehydroascorbic acid) is possible. VitaFast® Microbiological test • Samples with an added or natural vitamin content can be analysed • Method in conformity with official guidelines (Section 64 of the German Food & Feed Act, AOAC) • AOAC-RI certification for some VitaFast® tests • Ready-to-use reagents and standards for 96 determinations • Results available within 24 - 48 hours EASI-EXTRACT® Immunoaffinity columns • Isolation and concentration of the vitamin • Pigments and interfering compounds are removed • High recovery and low CV‘s • Single peaks RIDASCREEN® ELISA • Determination of total vitamin B12 content • Determination of added vitamin (folic acid , biotin) • One sample preparation procedure and one identical sample buffer for RIDASCREEN®FAST B12 and Folic Acid • Results within 1 hour • Ideal for process control 15 R-Biopharm AG – Product catalogue 2017 Vitamins VitaFast® Product Description No. of tests/amount Art. No. Microbiological microtiter plates VitaFast® Folsäure/Folic Acid AOAC-RI 100903 Quantitative determination of the total vitamin content (added and natural) or of the added vitamin only Limit of detection: 0.018 µg/100 g(ml) 96 determinations P1001 VitaFast® Vitamin B12 (Cyanocobalamin) AOAC-RI 101002 Quantitative determination of the total vitamin content (added and natural) or of the added vitamin only Limit of detection: 0.021 µg/100 g(ml) 96 determinations P1002 VitaFast® Vitamin B7 (Biotin) AOAC-RI 101001 Quantitative determination of the total vitamin content (added and natural) or of the added vitamin only Limit of detection: 0.013 µg/100 g(ml) 96 determinations P1003 VitaFast® Vitamin B3 Niacin Quantitative determination of the total vitamin content (added and natural) or of the added vitamin only Limit of detection: 0.0048 mg/100 g(ml) 96 determinations P1004 VitaFast® Pantothensäure/Pantothenic Acid AOAC-RI 100904 Quantitative determination of the total vitamin content (added and natural) or of the added vitamin only Limit of detection: 0.0035 mg/100 g(ml) 96 determinations P1005 VitaFast® Vitamin B1 (Thiamin) Quantitative determination of the total vitamin content (added and natural) or of the added vitamin only Limit of detection: 0.008 mg/100 g(ml) 96 determinations P1006 VitaFast® Vitamin B2 (Riboflavin) AOAC-RI 100902 Quantitative determination of the total vitamin content (added and natural) or of the added vitamin only Limit of detection: 0.0018 mg/100 g(ml) 96 determinations P1007 VitaFast® Vitamin B6 (Pyridoxin) Quantitative determination of the total vitamin content (added and natural) or of the added vitamin only Limit of detection: 0.0002 mg/100 g(ml) 96 determinations P1008 VitaFast® Inositol Quantitative determination of the total vitamin content (added and natural) Limit of detection: 0.5 mg/100 g(ml) 96 determinations P1009 50 determinations P1010 Enzymatic microtiter plate VitaFast® Vitamin C (L-Ascorbic Acid) Quantitative determination of vitamin C (L-ascorbic acid and L-dehydroascorbic acid) possible Limit of detection: 7.8 mg/100 g(ml) Spiking standards VitaFast® Folsäure/Folic Acid Spiking standard Folic Acid in solid form 3 vials P3001 VitaFast® Vitamin B12 (Cyanocobalamin) Spiking standard Cyanocobalamin in solid form 3 vials P3002 VitaFast® Vitamin B7 (Biotin) Spiking standard D-Biotin in solid form 3 vials P3003 VitaFast® Pantothensäure/Pantothenic Acid Spiking standard Ca-D-Pantothenat in solid form 3 vials P3005 1 vial for 50 sample preparations P2002 Enzyme VitaFast® Chicken Pancreatin 16 Enzyme for sample preparation for determination of natural folic acid Vitamins EASI-EXTRACT® Product Description No. of tests/amount Art. No. Immunoaffinity columns EASI-EXTRACT® VITAMIN B12 Immunoaffinity columns for sample clean-up prior to the analysis of vitamin B12 using HPLC or LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRP80 RBRP80B EASI-EXTRACT® VITAMIN B12 (LGE) AOAC „First Action“ certified Immunoaffinity columns for sample clean-up prior to the analysis of vitamin B12 using HPLC or LC-MS/MS 10 columns (10 ml format) 50 columns (10 ml format) RBRP88 RBRP88B EASI-EXTRACT® FOLIC ACID Immunoaffinity columns for sample clean-up prior to the analysis of folic acid using HPLC or LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRP81 RBRP81B EASI-EXTRACT® BIOTIN Immunoaffinity columns for sample clean-up prior to the analysis of biotin using HPLC or LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRP82 RBRP82B EASI-EXTRACT® MULTI-VIT B (LGE) Immunoaffinity columns for sample clean-up prior to the analysis of biotin, vitamin B12 and folic acid using HPLC 10 columns (10 ml format) 50 columns (10 ml format) RBRP183 RBRP183B RIDASCREEN® ELISA microtiter plates RIDASCREEN®FAST Vitamin B12 Enzyme immunoassay for quantitative analysis of total vitamin B12 in fortified food and vitamin products Limit of detection: 0.5 µg/kg 48 determinations Incubation time: 25 min R2103 RIDASCREEN® Biotin Enzyme binding assay for quantitative analysis of biotin in fortified food and vitamin products Detection limit: 0.37 - 5.5 µg/kg depending on matrix 96 determinations Incubation time: 1 hr 30 min R2201 RIDASCREEN®FAST Folsäure (Folic Acid) Enzyme immunoassay for quantitative analysis of added folic acid in fortified food and vitamin products Limit of detection: 0.5 µg/kg 48 determinations Incubation time: 25 min R3203 17 R-Biopharm AG – Product catalogue 2017 Mycotoxin analysis in food and feed Mycotoxins are toxic secondary metabolites produced by fungi (moulds). Mycotoxins can be formed in agricultural products, such as cereals, and can also occur in related food, meat and dairy products originating from farm animals. Due to the frequent occurrence of mycotoxins and their severe toxic effects on animals and humans, maximum levels (MLs) for the major mycotoxins have been set by legislative bodies. In accordance with these guidelines specific sample preparation and detection methods were developed. These include enzyme immunoassays, lateral flow devices or Immunoaffinity columns, etc. R-Biopharm assays for screening of mycotoxins in food and feed • RIDASCREEN® enzyme immunoassays (ELISAs) use the high specificity of antigen and antibody interaction to determine and quantify mycotoxins by photometric measurement. • RIDA®QUICK lateral flow tests are immunochromatographic tests for the determination of mycotoxins, semi-quantitative (visual evaluation) or quantitative (evaluation with RIDA®QUICK SCAN reader or RIDA®SMART APP software). 18 • Test cards, AFLACARD und OCHRACARD, allow a qualitative screening of mycotoxins at various levels in food and feed commodities. • Immunoaffinity columns (RIDA®, EASI-EXTRACT®, PREP®) use the high specificity of antigen and antibody interaction to isolate, purify and concentrate mycotoxins from many complex matrices prior to ELISA or chromatographic analysis. • Clean-up columns are Solid phase columns for the purification of mycotoxin contaminated samples prior to chromatographic analysis. RIDA®QUICK Lateral flow assay • Semi-quantitative or quantitative analysis • Fast and reliable New: Smartphone-based evaluation of all quantitative tests with RIDA®SMART APP RIDASCREEN® ELISA tests for up to 96 determinations • Highly sensitive • Specific RIDASCREEN®FAST ELISA for up to 48/96 determinations • Specific • Fast and reliable PREP®, EASI-EXTRACT®, RIDA® Immunoaffinity columns • Single or multi-toxin analysis in conjunction with HPLC, LC-MS/MS or ELISA • For a wide range of matrices Clean-up columns Solid phase columns • Rapid purification prior to HPLC, GC or LC-MS/MS 19 R-Biopharm AG – Product catalogue 2017 Mycotoxins Aflatoxins Product Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN® Aflatoxin M1 Enzyme immunoassay for quantitative determination of aflatoxin M1 in milk and milk powder* Detection limit: 5 ng/kg (milk/reconst. milk powder), 50 ng/kg (milk powder) 96 determinations Incubation time: 1 hr 15 min R1121 RIDASCREEN®FAST Aflatoxin M1 Enzyme immunoassay for quantitative determination of aflatoxin M1 in milk and milk powder Detection limit: < 125 ng/kg 48 determinations Incubation time: 15 min R5812 RIDASCREEN® Aflatoxin B1 30/15 Enzyme immunoassay for quantitative determination of aflatoxin B1 in cereals and feed Detection limit: 1 µg/kg (cereals), 1.7 µg/kg (soy), 2 µg/kg (dry cat food), 4 µg/kg (feed) 96 determinations Incubation time: 45 min R1211 RIDASCREEN® Aflatoxin Total Enzyme immunoassay for quantitative determination of total aflatoxin in cereals and feed* Detection limit: 1.75 µg/kg 96 determinations Incubation time: 45 min R4701 RIDASCREEN®FAST Aflatoxin Enzyme immunoassay for quantitative determination of aflatoxins in cereals and feed* Detection limit: 1.7 µg/kg 48 determinations Incubation time: 15 min R5202 RIDASCREEN®FAST Aflatoxin SC GIPSA/FGIS 2016-085 Enzyme immunoassay for quantitative determination of aflatoxins in cereals and feed Detection limit: 2 µg/kg 48 determinations Incubation time: 15 min R9002 Immunoaffinity columns AFLAPREP® Immunoaffinity columns for sample clean-up prior to the analysis of aflatoxins B1, B2, G1 and G2 using HPLC or LC-MS/MS 50 columns (1 ml format) RBRP07 AFLAPREP® M Immunoaffinity columns for sample clean-up prior to the analysis of aflatoxin M1 using HPLC or LC-MS/MS 25 columns (1 ml format) RBRP04 AFLAPREP® M WIDE Immunoaffinity columns for sample clean-up prior to the analysis of aflatoxin M1 using HPLC or LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRP124 RBRP124B EASI-EXTRACT® AFLATOXIN Immunoaffinity columns for sample clean-up prior to the analysis of aflatoxins B1, B2, G1 and G2 using HPLC or LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRRP71 RBRRP70N RIDA® Aflatoxin column Immunoaffinity columns for sample clean-up prior to ELISA 10 columns (1 ml format) 50 columns (1 ml format) R5001 R5002 50 columns (syringe format) RBRP25 Solid phase column PuriTox Aflatoxin * Further applications on request. 20 Solid phase column for sample clean-up prior to the analysis of total aflatoxins using HPLC or LC-MS/MS Mycotoxins Aflatoxins Product Description No. of tests/amount Art. No. Test strips RIDA®QUICK Aflatoxin Immunochromatographic test for the semi-quantitative determination (visual evaluation) of aflatoxin in cereals, soy flour, nuts, pistachios, coconut flour, sunflower seeds, figs, dates and cashew nuts Detection limit: 4, 10, 20 µg/kg 20 strips Incubation time: 4 - 16 min R5204 RIDA®QUICK Aflatoxin RQS FGIS/GIPSA 2013-048 Immunochromatographic test for the quantitative determination of aflatoxin in corn* in combination with RIDA®QUICK SCAN reader or RIDA®SMART APP software (page 77, Equipment/Accessories) Detection limit: 4 µg/kg 20 strips Incubation time: 5 min R5205 RIDA®QUICK Aflatoxin RQS ECO Immunochromatographic test with aqueous extraction for the quantitative determination of aflatoxin in corn in combination with RIDA®QUICK SCAN reader or RIDA®SMART APP software (page 77, Equipment/Accessories) Detection limit: 4 µg/kg 20 stripes Incubation time: 5 min R5206 Test cards AFLACARD B1 Qualitative detection of aflatoxin B1 at various screening levels 20 determinations RBRP27 AFLACARD TOTAL Qualitative detection of total aflatoxins at various screening levels 20 determinations RBRP38 Ochratoxin A ELISA microtiter plates RIDASCREEN® Ochratoxin A 30/15 Enzyme immunoassay for quantitative determination of ochratoxin A in cereals, feed, beer and pig serum* Detection limit: 1.25 μg/kg (cereals/feed), approx. 50 ng/kg (beer/pig serum) 96 determinations Incubation time: 45 min R1311 RIDASCREEN®FAST Ochratoxin A Enzyme immunoassay for quantitative determination of ochratoxin A in cereals and feed* Detection limit: 5 µg/kg 48 determinations Incubation time: 15 min R5402 Immunoaffinity columns OCHRAPREP® Immunoaffinity columns for sample clean-up prior to the analysis of ochratoxin A using HPLC or LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRP14 RBRP14B RIDA® Ochratoxin A column Immunoaffinity columns for sample clean-up prior to ELISA 10 columns (1ml format) R1303 50 columns (syringe format) RBRP13 20 determinations + 20 Immunoaffinity columns RBRP48 Solid phase column OCHRATOXIN CLEAN-UP COLUMNS Solid phase column for sample clean-up of specific matrices in conjunction with OCHRACARD Test cards OCHRACARD Qualitative detection of ochratoxin A at various screening levels * Further applications on request. 21 R-Biopharm AG – Product catalogue 2017 Mycotoxins Zearalenone Product Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN® Zearalenon Enzyme immunoassay for quantitative determination of zearalenone in cereals, feed, beer, serum and urine* Detection limits: 50 ng/l (serum/urine), 250 ng/l (beer), 1750 ng/kg (cereals/feed) 96 determinations Incubation time: 2 hrs 30 min R1401 RIDASCREEN®FAST Zearalenon Enzyme immunoassay for quantitative determination of zearalenone in cereals and feed* Detection limit: 17 - 41 µg/kg 48 determinations Incubation time: 15 min R5502 RIDASCREEN®FAST Zearalenon SC Enzyme immunoassay for quantitative determination of zearalenone in cereals Detection limit: 5 µg/kg 48 determinations Incubation time: 15 min R5505 10 columns (3 ml format) 50 columns (3 ml format) RBRRP91 RBRRP90 20 strips Incubation time: 5 min R5504 Immunoaffinity columns EASI-EXTRACT® ZEARALENONE Immunoaffinity columns for sample clean-up prior to the analysis of zearalenone using HPLC or LC-MS/MS Test strips RIDA®QUICK Zearalenon RQS Immunochromatographic test for the quantitative determination of zearalenone in corn in combination with RIDA®QUICK SCAN reader or RIDA®SMART APP software (page 77, Equipment/ Accessories) Detection limit: 75µg/kg (RIDA®QUICK SCAN), 50 µg/kg (RIDA®SMART APP) DON (Vomitoxin) ELISA microtiter plates RIDASCREEN® DON Enzyme immunoassay for quantitative determination of deoxynivalenol in cereals, malt, feed, beer and wort Detection limits: 18.5 µg/kg (cereals/malt/feed) and 3.7 µg/kg (beer/wort) 96 determinations Incubation time: 45 min R5906 RIDASCREEN®FAST DON AOAC RI 000701 & GIPSA/FGIS 2002-105 Enzyme immunoassay for quantitative determination of DON in cereals, malt and feed Detection limit: < 0.2 mg/kg 96 determinations 48 determinations Incubation time: 8 min R5901 R5902 RIDASCREEN®FAST DON SC GIPSA/FGIS 2014-052 Enzyme immunoassay for quantitative determination of DON in cereals, malt and feed Detection limit: 0.074 mg/kg 48 determinations Incubation time: 8 min R5905 10 columns (3 ml format) 50 columns (3 ml format) RBRP50 RBRP50B 20 strips Incubation time: 5 min R5904 Immunoaffinity columns DONPREP® Immunoaffinity columns for sample clean-up prior to the analysis of deoxynivalenol using HPLC or LC-MS/MS Test strips RIDA®QUICK DON 22 Immunochromatographic test for the determination of DON in grain: semi-quantitative (visual evaluation) or quantitative (evaluation with RIDA®QUICK SCAN reader or RIDA®SMART APP software, page 77, Equipment/Accessories) Detection limit: 0.5 mg/kg Mycotoxins Fumonisins Product Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN® Fumonisin Enzyme immunoassay for quantitative analysis of fumonisins in corn and corn products Detection limit: 25 µg/kg 96 determinations Incubation time: 45 min R3401 RIDASCREEN®FAST Fumonisin GIPSA/FGIS 2016-081 Enzyme immunoassay for quantitative determination of fumonisins in cereals and feed Detection limit: 0.222 mg/kg 48 determinations Incubation time: 15 min R5602 25 columns (3 ml format) RBRP31 20 strips Incubation time: 5 min R5606 Immunoaffinity columns FUMONIPREP® Immunoaffinity columns for sample clean-up prior to the analysis of fumonisins B1, B2 and B3 using HPLC or LC-MS/MS Test strips RIDA®QUICK Fumonisin RQS Immunochromatographic test for the quantitative determination of fumonisin in corn in combination with RIDA®QUICK SCAN reader or RIDA®SMART APP software (page 77, Equipment/ Accessories) Detection limit: 0.3 mg/kg T-2 Toxin ELISA microtiter plates RIDASCREEN® T-2 Toxin Enzyme immunoassay for quantitative determination of T-2 toxin in cereals and feed Detection limit: < 5 µg/kg 96 determinations Incubation time: 1 hr 30 min R3801 RIDASCREEN®FAST T-2 Toxin Enzyme immunoassay for quantitative determination of T-2 toxin in cereals and feed Detection limit: < 20 µg/kg 48 determinations Incubation time: 15 min R5302 96 determinations Incubation time: 45 min R3805 10 columns (3 ml format) 50 columns (3 ml format) RBRP43 RBRP43B 20 strips Incubation time: 5 min R5304 T-2 / HT-2 Toxin ELISA microtiter plates RIDASCREEN® T-2 / HT-2 Toxin Enzyme immunoassay for quantitative determination of T-2/HT-2 toxin in oats, corn, barley and wheat Detection limit: 16 μg/kg (oats), 12 μg/kg (corn), 21 μg/kg (wheat), 33 μg/kg (barley) Immunoaffinity columns EASI-EXTRACT® T-2 & HT-2 Immunoaffinity columns for sample clean-up prior to the analysis of T-2 and HT-2 using HPLC or LC-MS/MS Test strips RIDA®QUICK T-2 / HT-2 RQS Immunochromatographic test for or quantitative determination of T-2/HT-2 toxin in oats, corn, and wheat in combination with RIDA®QUICK SCAN reader or RIDA®SMART APP software (page 77, Equipment/ Accessories) Detection limit: 50 µg/kg 23 R-Biopharm AG – Product catalogue 2017 Mycotoxins Citrinin Product Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN®FAST Citrinin Enzyme immunoassay for quantitative determination of citrinin in cereals and feed Detection limit: 15 µg/kg 48 determinations Incubation time: 25 min R6302 25 columns (3 ml format) RBRP126 Immunoaffinity columns EASI-EXTRACT® CITRININ Immunoaffinity columns for sample clean-up prior to the analysis of citrinin using HPLC or LC-MS/MS Multi Toxin Immunoaffinity columns DZT MS-PREP® Immunoaffinity columns for sample clean-up prior to the analysis of deoxynivalenol, zearalenone, T-2 and HT-2 using LC-MS/MS 10 columns (1 ml format) 50 columns (1 ml format) RBRP73 RBRP73B AFLAOCHRA PREP® Immunoaffinity columns for sample clean-up prior to the analysis of total aflatoxins and ochratoxin A using HPLC or LC-MS/MS 10 columns (1 ml format) 50 columns (1 ml format) RBRP89 RBRP89B AOF MS-PREP® Immunoaffinity columns for sample clean-up prior to the analysis of total aflatoxins, ochratoxin A and fumonisin using LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRP115 RBRP115B AO ZON PREP® Immunoaffinity columns for sample clean-up prior to the analysis of total aflatoxins, ochratoxin A and zearalenone using HPLC or LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRP112 RBRP112B Solid phase columns PuriTox AflaZON Solid phase column for sample clean-up prior to the analysis of total aflatoxins and zearalenone using HPLC or LC-MS/MS 25 columns (syringe format) TC-M160 PuriTox Total Myco-MS Solid phase column for sample clean-up prior to the analysis of total aflatoxins, ochratoxin A, DON, 3-acetyl DON, 15-acetyl DON, ZON, T-2, HT-2, FB1, FB2 and FB3 using LC-MS/MS 25 columns (syringe format) TC-MT3000 Trichothecene Solid phase columns Trichothecene P columns Solid phase column for sample clean-up prior to the analysis of trichothecenes using GC or LC-MS/MS 30 columns (test tube format) RBRP51 PuriTox Trichothecene Solid phase column for clean-up prior to the analysis of trichothecenes using GC or LC-MS/MS 25 columns (syringe format) TC-T220 PuriTox DON/NIV Solid phase column for clean-up prior to the analysis of deoxynivalenol and nivalenol using GC or LC-MS/MS 25 columns (gravity flow format) TC-C210 24 Patulin Product Description No. of tests/amount Art. No. Enzyme Pectinase An enzyme for the clarification of cloudy apple juice and apple purée prior to patulin analysis 100 determinations RBRP129 10 columns (3 ml format) 50 columns (3 ml format) RBRP250 RBRP250B 10 columns (3 ml format) 50 columns (3 ml format) RBRP125 RBRP125B Molecularly imprinted columns EASIMIP™ PATULIN Molecularly imprinted columns for sample clean-up prior to the analysis of patulin using HPLC Sterigmatocystin Immunoaffinity columns EASI-EXTRACT® STERIGMATOCYSTIN Immunoaffinity columns for sample clean-up prior to the analysis of sterigmatocystin using HPLC 25 R-Biopharm AG – Product catalogue 2017 Automated online analysis of mycotoxins in food and feed IMMUNOPREP® ONLINE immunoaffinity cartridges are used together with the RIDA®CREST or RIDA®CREST ICE handling system to combine automated online sample preparation with quantitative analysis of the mycotoxin of interest. The immunoaffinity cartridge contains a monoclonal antibody that is specific for the mycotoxin, coupled to a hydrophilic polymer that can withstand high pressure. The RIDA®CREST or RIDA®CREST ICE system enables the use of the IMMUNOPREP ONLINE cartridges to be incorporated directly with HPLC, UHPLC or LC-MS/MS systems. The IMMUNOPREP® ONLINE cartridge offers highly specific, sensitive, rapid and automated analysis. The sample application, washing and elution is performed online for up to 12 samples before the cartridge is automatically removed and replaced with a new one. This level of reuse has been found to offer optimum cartridge performance and removes the chance of interference or carryover. Following extraction of the toxin from the sample with solvent, the extract is filtered, diluted and transferred to an autosampler vial. The diluted extract is injected onto the immunoaffinity cartridge and any toxin present in the sample is retained by antibody in the cartridge. Unbound matrix material is then automatically removed by washing the cartridge and the resulting 26 wash goes to waste. Subsequently the toxins are released from the antibody following online elution with the mobile phase and the complete elution fraction from the cartridge is quantitatively analysed for the mycotoxin of interest. IMMUNOPREP® ONLINE • • • • • • Improved Quality Assurance Improved Traceability and Efficiency Reusable cartridges Increased sample throughput Potential cost savings New platform technology: RIDA®CREST or RIDA®CREST ICE Mycotoxins Automated online analysis Product Description Aflatoxin Online immunoaffinity cartridges No. of tests/amount Art. No. IMMUNOPREP® ONLINE AFLATOXIN Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of aflatoxins B1, B2, G1 and G2 with HPLC 48 cartridges 96 cartridges RBRP900/48 RBRP900 IMMUNOPREP® ONLINE AFLATOXIN M1 Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of aflatoxins M1 with HPLC 48 cartridges 96 cartridges RBRP904/48 RBRP904 Ochratoxin A Online immunoaffinity cartridges IMMUNOPREP® ONLINE OCHRATOXIN Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of ochratoxin A with HPLC 48 cartridges 96 cartridges RBRP901/48 RBRP901 DON (Vomitoxin) Online immunoaffinity cartridges IMMUNOPREP® ONLINE DEOXYNIVALENOL Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of deoxynivalenol with HPLC 48 cartridges 96 cartridges RBRP902/48 RBRP902 27 R-Biopharm AG – Product catalogue 2017 Trilogy® certified mycotoxin reference materials and mycotoxin standards Trilogy® Analytical Laboratory offers certified reference materials for the quality assurance of mycotoxin analysis. Trilogy® certified mycotoxin reference materials are naturally contaminated homogeneous products that have been certified to contain a specific concentration of one or more mycotoxins. These reference materials have various form. The Trilogy® standards can be used applications including daily quality assurance, technician training, troubleshooting, proficiency testing, quality documentation and method validation. Reference materials are available containing the major mycotoxins in various matrices and levels of contamination: Aflatoxin, Ochratoxin, Zearalenone, Deoxynivalenol and Fumonisin contaminated material are available, as well as multi-toxin containing reference materials. Commodities include corn and corn by-products, wheat and wheat products, barley and malted barley, oats, rice and coffee as well as complex products like animal feed. Samples are available in 100 g, 500 g and 1 kg re-sealable foil packs. for spiking experiments in order to check laboratory performance or for the analysis of mycotoxins by HPLC, GC or LC-MS/MS. The Trilogy® dried standards are very easy to use. A simple reconstitution step reduces the need to handle hazardous mycotoxin powders. Trilogy® also provides a wide range of analytical standards for over 30 different mycotoxins, both in solvents and in dry 28 The Trilogy® liquid standards are ready to use and contain mycotoxins in dissolved specified organic solvents. They are both intended for use by customers who do not have a spectrophotometer or for those who want to ensure accurate HPLC/LC-MS/MS determination of mycotoxins with minimal preparation and effort. Shelf life for both types of mycotoxin standards is typically 12 months after production; in addition to that the Trilogy® dried standards have a 6 months shelf life after reconstitution. Trilogy® certified Mycotoxin reference material • Naturally contaminated materials • Single and multi-toxins • Cereal, complex materials such as feed Trilogy® mycotoxin standard substances • Dried standard substances • Ready-to-use standards • Single toxin and toxin groups 29 R-Biopharm AG – Product catalogue 2017 Mycotoxins Mycotoxin standards Product Description Aflatoxins dried No. of tests/amount Art. No. Trilogy® Dried Standard Aflatoxins B1, B2, G1, G2 Aflatoxins B1, B2, G1, G2 (4:1:4:1) (2/0.5/2/0.5 µg/ml) 5 µg/ml after reconstitution TS-108 Trilogy® Dried Standard Aflatoxin B1 Aflatoxin B-1 25 µg/ml after reconstitution TS-104 Trilogy® Dried Standard Aflatoxin B2 Aflatoxin B-2 25 µg/ml after reconstitution TS-105 Trilogy® Dried Standard Aflatoxin G1 Aflatoxin G-1 25 µg/ml after reconstitution TS-106 Trilogy® Dried Standard Aflatoxin G2 Aflatoxin G-2 25 µg/ml after reconstitution TS-107 Trilogy® Dried Standard Aflatoxin M1 Aflatoxin M1 1 µg/ml after reconstitution TS-130 liquid Trilogy® Liquid Standard Aflatoxin B1, B2, G1, G2 Aflatoxin B1, B2, G1, G2 (4:1:4:1), 5 µg/ml (2/0.5/2/0.5 µg/ml) in acetonitril 10 ml TSL-108 AFLASTANDARD Total aflatoxin standard (B1, B2, G1, G2) solution at 1000 ng/ml (250 ng/ml each) in methanol 6 ml 3 ml RBRP22 RBRP22A Trilogy® Liquid Standard Aflatoxin B1 Aflatoxin B1; 25 µg/ml in acetonitrile 10 ml TSL-104 Trilogy® Liquid Standard Aflatoxin B2 Aflatoxin B2; 25 µg/ml in acetonitrile 10 ml TSL-105 Trilogy® Liquid Standard Aflatoxin G1 Aflatoxin G1; 25 µg/ml in acetonitrile 10 ml TSL-106 Trilogy® Liquid Standard Aflatoxin G2 Aflatoxin G2; 25 µg/ml in acetonitrile 10 ml TSL-107 Trilogy® Liquid Standard Aflatoxin M1 Aflatoxin M1; 0.5 µg/ml in acetonitrile 2 ml TSL-143 M1 STANDARD Aflatoxin M1 standard solution at a concentration of 1000 ng/ml in acetonitrile 6 ml RBRP42 Ochratoxin A dried Trilogy® Dried Standard Ochratoxin A Ochratoxin A 1 µg/ml after reconstitution TS-503 liquid Trilogy® Liquid Standard Ochratoxin Ochratoxin A; 10 µg/ml in methanol 5 ml TSL-504 OCHRASTANDARD Ochratoxin A standard solution at a concentration of 1000 ng/ml in methanol 6 ml 3 ml RBRP11 RBRP11A 30 Mycotoxins Mycotoxin standards Product Description Zearalenone dried Trilogy® Dried Standard Zearalenon Zearalenone No. of tests/amount Art. No. 25 µg/ml after reconstitution TS-401 liquid Trilogy® Liquid Standard Zearalenon Zearalenone; 25 µg/ml in methanol 10 ml TSL-401 ZEASTANDARD Zearalenone standard solution at a concentration of 1000 ng/ml in acetonitrile 3 ml RBRP44A DAS dried Trilogy® Dried Standard Diacetoxyscirpenol (DAS) Diacetoxyscirpenol (DAS) 100 µg/ml after reconstitution TS-316 DON (Vomitoxin) dried Trilogy® Dried Standard DON Deoxynivalenol 50 µg/ml after reconstitution TS-310 Trilogy® Dried Standard Deoxynivalenol (DON) Deoxynivalenol (DON) 100 µg/ml after reconstitution TS-317 Trilogy® Dried Standard 3-Acetyl Deoxynivalenol 3-Acetyl Deoxynivalenol 100 µg/ml after reconstitution TS-342 Trilogy® Dried Standard 15-Acetyl Deoxynivalenol 15-Acetyl Deoxynivalenol 100 µg/ml after reconstitution TS-343 10 ml TSL-317 100 µg/ml after reconstitution TS-351 100/30 µg/ml after reconstitution TS-202 liquid Trilogy® Liquid Standard Deoxynivalenol (DON) Deoxynivalenol (DON); 100 µg/ml in methanol Fusarenon X dried Trilogy® Dried Standard Fusarenon X Fusarenon X Fumonisins dried Trilogy® Dried Standard Fumonisin B1, B2 Fumonisin B1, Fumonisin B2 (10:3) liquid Trilogy® Liquid Standard Fumonisin B1, B2 Fumonisin B1, Fumonisin B2 100 µg/ml Fumonisin B1, 30 µg/ml Fumonisin B2 in acetonitrile/water (50/50) 2 ml TSL-202 Trilogy® Liquid Standard Fumonisin B1 Fumonisin B1; 100 µg/ml in acetonitrile/water (50/50) 5 ml TSL-204 Trilogy® Liquid Standard Fumonisin B2 Fumonisin B2; 100 µg/ml in acetonitrile/water (50/50) 2 ml TSL-205 Neosolaniol dried Trilogy® Dried Standard Neosolaniol Neosolaniol 100 µg/ml after reconstitution TS-328 Nivalenol dried Trilogy® Dried Standard Nivalenol Nivalenol 100 µg/ml after reconstitution TS-344 31 R-Biopharm AG – Product catalogue 2017 Mycotoxins Mycotoxin standards Product Description T-2/HT-2 dried No. of tests/amount Art. No. Trilogy® Dried Standard T-2 Toxin T-2 Toxin 100 µg/ml after reconstitution TS-314 Trilogy® Dried Standard HT-2 Toxin HT-2 Toxin 100 µg/ml after reconstitution TS-333 liquid Trilogy® Liquid Standard T-2 Toxin T-2 Toxin; 100 µg/ml in acetonitrile 5 ml TSL-314 Trilogy® Liquid Standard HT-2 Toxin HT-2 Toxin; 100 µg/ml in acetonitrile 5 ml TSL-333 Trichotehecenes - Multitoxins liquid Trilogy® Liquid Standard Type A & B Trichothecenes Type A & B Trichothecenes; (Fusarenon X, Deoxynivalenol, Nivalenol, 3- & 15-Acetyl DON, HT-2 Toxin, Diacetoxyscirpenol, T-2 Toxin, Neosolaniol) 100 µg/ml in acetonitrile 2 ml TSL-307 dried Trilogy® Dried Standard Type A Trichothecenes Type A Trichothecenes (Diacetoxyscirpenol, HT-2 Toxin, T-2 Toxin, Neosolaniol) 10 µg/ml after reconstitution TS-353 Trilogy® Dried Standard Type B Trichothecenes Type B Trichothecenes (Fusarenon X, Deoxynivalenol, 3- & 15-Acetyl DON, Nivalenol) 10 µg/ml after reconstitution TS-354 Citrinin dried Trilogy® Dried Standard Citrinin Citrinin 5 µg/ml after reconstitution TS-904 Cyclopiazonic acid dried Trilogy® Dried Standard Cyclopiazonic Acid Cyclopiazonic acid 10 µg/ml after reconstitution TS-802 Patulin liquid Trilogy® Liquid Standard Patulin Patulin; 25 µg/ml in acetonitrile 5 ml TSL-601 32 Mycotoxins Reference material for mycotoxin analysis Product Description Reference material Food or feed product No. of tests/amount Art. No. Trilogy® Certified Reference Material Aflatoxin Commodities available upon request 100 gram 500 gram 1000 gram TR-A100 TR-A500 TR-A1000 Trilogy® Certified Reference Material Ochratoxin Commodities available upon request 100 gram 500 gram 1000 gram TR-O100 TR-O500 TR-O1000 Trilogy® Certified Reference Material Zearalenon Commodities available upon request 100 gram 500 gram 1000 gram TR-Z100 TR-Z500 TR-Z1000 Trilogy® Certified Reference Material Deoxynivalenol Commodities available upon request 100 gram 500 gram 1000 gram TR-D100 TR-D500 TR-D1000 Trilogy® Certified Reference Material Fumonisin Commodities available upon request 100 gram 500 gram 1000 gram TR-F100 TR-F500 TR-F1000 Trilogy® Certified Reference Material Multitoxin Commodities and mycotoxins available upon request 100 gram 500 gram 1000 gram TR-MT100 TR-MT500 TR-MT1000 Trilogy® Certified Reference Material Complex commodities; Single & Multitoxin Commodities and mycotoxins available upon request 100 gram 500 gram 1000 gram TR-CC100 TR-CC500 TR-CC1000 33 R-Biopharm AG – Product catalogue 2017 Analysis of hormone & anabolic residues in food Hormones and anabolics can be used as growth promoters in livestock breeding to enhance average daily weight gain and meat/fat ratio. As a consequence, hormone and anabolic residues can occur in food of animal origin. Due to their systemic function, hormonal residues in food bear a potential health risk for the consumer. Additionally, the entry of hormonal active substances into surface and ground water can have an ecological impact on aquatic ecosystems. RIDASCREEN® • ELISAs for the most commonly used hormones and anabolics • Quantitative Screening • Applications for many matrices • Evaluation with RIDA®SOFT Win 34 Consequently, most countries have banned the use of hormones and anabolics in livestock breeding completely with exceptions for veterinary purposes. Hormones & Anabolics Product Description β-Agonists ELISA microtiter plates No. of tests/amount Art. No. RIDASCREEN® β-Agonists Enzyme immunoassay for quantitative analysis of β-agonists in urine (150 ng/l), serum (900 ng/l), meat (100 ng/kg), liver (130 ng/kg), milk (45 ng/l), feed (1000 ng/kg) 96 determinations Incubation time: 1 hr R1704 RIDASCREEN® Clenbuterol Enzyme immunoassay for quantitative analysis of clenbuterol in milk (50 ng/l), meat (100 ng/kg), liver (150 ng/kg), kidney (200 ng/kg), urine (100 ng/l), plasma/serum (250 ng/l), hair (2 µg/kg), eye ball (200 ng/kg), feed (600 µg/kg) 96 determinations Incubation time: 1 hr R1711 RIDA® β-Agonists & Clenbuterol Spiking Solution 100 ng/ml 1 ml R1799 Clenbuterol Assay Control (positive) Freeze-dried calves urine positive for clenbuterol 1 x 5 ml R1707 Clenbuterol Assay Control (negative) Freeze-dried calves urine negative for clenbuterol 1 x 2 ml R1708 RIDASCREEN® Ractopamin Enzyme immunoassay for quantitative analysis of ractopamine in urine (600 ng/l), meat (200 ng/kg), liver (300 ng/kg), * 96 determinations Incubation time: 1 hr 30 min R9901 RIDA® Ractopamin Spiking Solution 10 ng/ml 1 ml R9999 Stilbenes ELISA microtiter plates RIDASCREEN® DES Enzyme immunoassay for quantitative analysis of DES in urine (200 ng/l), meat/feces (100 ng/kg), bile (2 µg/l), * 96 determinations Incubation time: over night and 1 hr 30 min R2701 RIDA® DES Spiking Solution 10 ng/ml 1 ml R2799 DES Assay Control (positive) Freeze-dried calves urine positive for DES 1 x 5 ml R2707 DES Assay Control (negative) Freeze-dried calves urine negative for DES 1 x 5 ml R2708 Sex hormones ELISA microtiter plates RIDASCREEN® 17β-Östradiol Enzyme immunoassay for quantitative analysis of 17 β-estradiol in bovine plasma (20 ng/l), * 96 determinations Incubation time: 2 hrs 30 min R2301 RIDA® 17β-Östradiol Spiking Solution 200 ng/ml 1 ml R2399 RIDASCREEN® Testosteron Enzyme immunoassay for quantitative analysis of testosterone in bovine plasma (20 ng/l), * 96 determinations Incubation time: 2 hrs 30 min R2401 RIDA® Testosteron Spiking Solution 500 ng/ml 1 ml R2499 * Further applications on request. 35 R-Biopharm AG – Product catalogue 2017 Hormones & anabolics Product Description Gestagens ELISA microtiter plates RIDASCREEN® Acetylgestagene Enzyme immunoassay for quantitative analysis of medroxyprogesterone acetate in bovine perirenal fat (300 ng/kg) 96 determinations Incubation time: 2 hrs 30 min R1801 RIDA® Acetylgestagene Spiking Solution 100 ng/ml medroxyprogesterone acetate 1 ml R1899 RIDASCREEN® Melengestrolacetat Enzyme immunoassay for quantitative analysis of melengestrolacetate in bovine renal fat (300 ng/kg), meat (75 ng/kg) 96 determinations Incubation time: 2 hrs 30 min R6502 RIDA® Melengestrolacetat Spiking Solution 100 ng/ml 1 ml R6599 Anabolic steroids ELISA microtiter plates RIDASCREEN® Trenbolon Enzyme immunoassay for quantitative analysis of trenbolone in urine (400 ng/l), bile (1 µg/l), meat/liver (200 ng/kg), feces (25 ng/kg), * 96 determinations Incubation time: 2 hrs 30 min R2601 RIDA® Trenbolon Spiking Solution 50 ng/ml 1 ml R2699 Trenbolone Assay Control (negative) Freeze-dried calves urine negative for trenbolone 1 x 5 ml R2608 RIDASCREEN® Methyltestosteron Enzyme immunoassay for quantitative analysis of methyltestosterone in porcine urine (540 ng/l), bovine urine (750 ng/l), beef (450 ng/kg), pork (390 ng/kg), fish (430 ng/kg), porcine liver (180 ng/kg), bovine liver (720 ng/kg) 96 determinations Incubation time: 2 hrs 15 min R3611 RIDA® Methyltestosteron Spiking Solution 100 ng/ml 1 ml R3699 RIDASCREEN® 19-Nortestosteron Enzyme immunoassay for quantitative analysis of 19-nortestosterone in urine (3 µg/l), * 96 determinations Incubation time: 1 hr 15 min R2801 RIDA® 19-Nortestosteron Spiking Solution 1 µg/ml 1 ml R2899 RIDASCREEN® Ethinylöstradiol Enzyme immunoassay for quantitative analysis of ethinylestradiol in bovine/porcine urine (370 ng/l), beef (230 ng/kg), pork (200 ng/kg), bovine plasma (50 ng/l) 96 determinations Incubation time: 2 hrs 30 min R2511 RIDA® Ethinylöstradiol Spiking Solution 20 ng/ml 1 ml R2599 Non-steroidal compounds ELISA microtiter plates RIDASCREEN® Zeranol Enzyme immunoassay for quantitative analysis of zeranol in urine (1.5 µg/l), * 96 determinations Incubation time: 1 hr 15 min R3301 RIDA® Zeranol Spiking Solution 20 ng/ml 1 ml R3399 Accessories Solid phase columns RIDA® C18 columns Solid phase extraction columns for use in conjunction with RIDASCREEN® ELISAs 100 columns R2002 * Further applications on request. 36 No. of tests/amount Art. No. 37 R-Biopharm AG – Product catalogue 2017 Analysis of antibiotic residues In addition to their function as veterinary drugs, antibiotics can be used as antimicrobial growth promoters in livestock breeding. As a consequence of incorrect or illegal use, antibiotic drug residues in food of animal origin can remain. Because of the potentially toxic, carcinogenic and allergic properties of antibiotic residues, contaminated food is a direct health risk for consumers. Additionally, the inappropriate use of antibiotics in animal husbandry and food production can promote multi-resistant pathogens, which pose an increasing risk for public health. 38 For these reasons, most countries have established Maximum Residue Limits (MRLs) and monitoring programs for antibiotic residues in food. Non-compliance with these legislations e.g. in export can lead to severe penalties. For biotechnological industries, antibiotic residues bear additionally a technological and economic risk, as they can inhibit production processes involving microorganisms and thus lead to production losses. RIDASCREEN® ELISAs for qualitative screening • Quantitative results of single antibiotics or antibiotic groups • Detects the most commonly used antibiotics • Applications for a wide range of matrices • Evaluation with RIDA®SOFT Win EASI-EXTRACT®/RIDA® Immunoaffinity columns for sample clean-up • For ELISA, HPLC or LC-MS/MS-analysis • For complex matrices such as honey • Reduced interferences • Excellent recovery Premi®Test Microbial inhibition test for qualitative screening • Detects a broad spectrum of antibiotics • Easy to handle, no sophisticated equipment needed • Fast (-er than plate tests) • Sensitive (in conformity with EU-MRLs) • Validated (AOAC-RI PTMSM and AFNOR NF VALIDATION) 39 R-Biopharm AG – Product catalogue 2017 Antibiotics Product Description Fenicols ELISA microtiter plates RIDASCREEN® Chloramphenicol Enzyme immunoassay for quantitative analysis of chloramphenicol in milk (24 ng/l), milk powder (25 ng/kg), joghurt/kefir/buttermilk/cream (12 ng/kg), curd/sour cream (15 ng/kg), butter (61 ng/kg), cheese (16 ng/kg), honey (25 ng/kg), royal jelly (23 ng/kg), meat (5 ng/kg), fish/shrimp (8 ng/kg), egg (15 ng/kg), urine (196 ng/l), plasma/serum (18 ng/l), feed (107 ng/kg) 96 determinations Incubation time: 45 min R1511 RIDA® Chloramphenicol Spiking Solution 50 ng/ml 1 ml R1599 Fenicols Immunoaffinity columns EASI-EXTRACT® CHLORAMPHENICOL Immunoaffinity columns for sample clean-up prior to the analysis of chloramphenicol using HPLC or LC-MS/MS 10 columns (3 ml format) 50 columns (3 ml format) RBRP300 RBRP300B Tetracyclins ELISA microtiter plates RIDASCREEN® Tetracyclin Enzyme immunoassay for quantitative analysis of tetracycline in milk (0.9 µg/l), milk powder (5 µg/kg), cheese (2.3 µg/kg), butter (2.6 µg/kg), dairy products (1 µg/kg), honey (3.7 µg/kg), meat (1.5 µg/kg), sausage (4.6 µg/kg), fish (1.5 µg/kg), shrimp (1.2 µg/kg), eggs (2.8 µg/kg) 96 determinations Incubation time: 1 hr 30 min R3505 RIDA® Tetracyclin Spiking Solution Lyophilisate, produces 10 ml of a 100 ng/ml stock solution 1 lyophilisate, 1 reconstitution buffer R3599 β-Lactame ELISA microtiter plates RIDASCREEN® Penicillin Enzyme immunoassay for quantitative analysis of penicillins milk (0.2 µg/l), cheese/butter/yoghurt/curd/cream/sour cream/kefir/whey (0.9 - 2.1 µg/l or µg/kg), infant formula (0.3 µg/l), serum (0.4 µg/l), meat (2.6 µg/kg) 96 determinations Incubation time: 1 hr 30 min R2921 40 No. of tests/amount Art. No. Antibiotics Product Description Nitrofurans ELISA microtiter plates No. of tests/amount Art. No. RIDASCREEN® Nitrofuran (AOZ) Enzyme immunoassay for quantitative analysis of AOZ in shrimp/fish/milk (50 ng/kg), meat/liver/whole egg/honey (100 ng/kg), * 96 determinations Incubation time: 1 hr 15 min R3703 RIDA® Nitrofuran (AOZ) Spiking Solution 20 ng/ml 1 ml R3798 RIDASCREEN® Nitrofuran (AMOZ) Enzyme immunoassay for quantitative analysis of AMOZ in shrimp/meat/liver/fish/whole egg (200 ng/kg), * 96 determinations Incubation time: 1 hr 15 min R3711 RIDA® Nitrofuran (AMOZ) Spiking Solution 20 ng/ml 1 ml R3799 RIDASCREEN® Nitrofuran (AHD) Enzyme immunoassay for quantitative analysis of AHD in shrimp (200 ng/kg), fish (76 ng/kg), * 96 determinations Incubation time: 1 hr 15 min R3713 RIDA® Nitrofuran (AHD) Spiking Solution 20 ng/ml 1 ml R3796 RIDASCREEN® Nitrofuran (SEM) Enzyme immunoassay for quantitative analysis of SEM in meat beef/pork/shrimp (300 ng/kg), poultry (400 ng/kg), fish 360 ng/kg), * 96 determinations Incubation time: 1 hr 15 min R3715 RIDA® Nitrofuran (SEM) Spiking Solution 20 ng/ml 1 ml R3797 Aminoglycosides ELISA microtiter plates RIDASCREEN® Streptomycin Enzyme immunoassay for quantitative analysis of streptomycin in milk (5 µg/l), honey (2 µg/kg), beef/pork (22 µg/kg), poultry (28 µg/kg), liver (23 (µg/kg), kidney (18 µg/kg), shrimp (20 µg/kg), apple juice (4 µg/l) 96 determinations Incubation time: 1 hr 15 min R3103 RIDA® Streptomycin Spiking Solution 10 μg/ml 1 ml R3199 Sulfonamides ELISA microtiter plates RIDASCREEN® Sulfamethazin Enzyme immunoassay for quantitative analysis of sulfamethazine in milk (4 µg/l), meat/kidney (18 µg/kg), * 96 determinations Incubation time: 2 hrs 30 min R3001 RIDA® Sulfamethazin Spiking Solution 10 µg/ml 1 ml R3098 RIDASCREEN® Sulfonamide Enzyme immunoassay for quantitative analysis of sulfonamides in poultry/egg (1.5 µg/kg), pork/fish/shrimps/honey (2 µg/kg), milk (3.5 µg/l) 96 determinations Incubation time: 1 hr 15 min R3004 RIDA® Sulfonamide/ Sulfamethoxypyridazin Spiking Solution 0.1 μg/ml 1 ml R3099 * Further applications on request. 41 R-Biopharm AG – Product catalogue 2017 Antibiotics Product Description Quinolones ELISA microtiter plates RIDASCREEN® Chinolone/Quinolones Enzyme immunoassay for quantitative analysis of quinolones in shrimp (6 µg/kg), fish (8 µg/kg), egg (9 µg/kg), meat (10 µg/kg), * 96 determinations Incubation time: 1 hr 15 min R3113 RIDA® Ciprofloxacin Spiking Solution 1 µg/ml 1 ml R3198 Polypeptides ELISA microtiter plates RIDASCREEN® Bacitracin Enzyme immunoassay for quantitative analysis of bacitracin in milk (11 µg/l), meat (9 µg/kg), eggs (11 µg/kg), feed (82 µg/kg), urine (23 µg/l) 96 determinations Incubation time: 1 h 30 min R2901 Premi®Test Test ampoules Premi®Test Microbial inhibition test for the screening of antibiotic residues in food of animal origin such as meat (beef, pork, poultry), liver, kidney, fish, shrimp, eggs, bovine/porcine urine and pork/poultry feed Detectable antibiotic groups: β-lactams, cephalosporins, macrolides, tetracyclins, sulphonamides, aminoglycosides, quinolones, polypeptides, fenicols, others 4 x 25 ampoules 25 ampoules Incubation time: 3 hrs R3900 R3925 Premi®Test Reagent Premi®Test Urin Reagent for the preparation of bovine and porcine urine samples for the screening of antibiotic residues with Premi®Test 1 dropper bottle containing 15 ml, sufficient for 30 samples with a sample volume of 30 ml each R3921 * Further applications on request. 42 No. of tests/amount Art. No. Phycotoxins Product Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN® FAST PSP SC Enzymimmunoassay for quanitative analysis of saxitoxin and related algae toxins in mussels; Detection limit: 50 µg/kg 48 determinations Incubation time: 30 min R1905 48 determinations Incubation time: 1 hr 30 min R4302 Food adulteration ELISA microtiter plates RIDASCREEN® CIS Enzyme immunoassay for the analysis of cow‘s milk (bovine IgG) in sheep and goat’s milk and cheese; Detection limit: 0.1 % (cow‘s milk in sheep/goat‘s milk/ cheese) Test strips RIDA®QUICK CIS Immunochromatographic test for the detection of cow’s milk (bovine IgG) in milk or cheese of other species; Detection limit: 0.5 % (cow’s milk in sheep/goat’s milk/ cheese) 25 strips Incubation time: 5 min R4303 DUROTEST® S Membrane strips for detection of non-durum wheat adulteration in semolina Detection limit: 3 % non-durum wheat. 20 strips (80 determinations) RBRP10 25 columns RBRP111 96 determinations Incubation time: 15 min R1605 1 Set (200 wine samples) R1699 96 determinations 48 determinations Incubation time: 1 hr 15 min R1601 R1604 48 determinations Incubation time: 5 min R1603 Solid phase column MELAMINE CLEAN-UP COLUMN Solid phase column for sample clean-up prior to the analysis of melanine using HPLC or LC-MS/MS Detection limit: 15.625 ng/ml melamine Histamine Histamine Enzymatic test microtiter plates RIDASCREEN® Histamine (enzymatic) Enzymatic test in microtiter plate format for the quantitative determination of histamine in fish, canned fish, fish meal, wine, cheese and milk; for the sample preparation of wine it is recommended to use RIDA® Sample Decolorant (R1699) Detection limit: 0.75 - 3.75 mg/kg (ppm) histamine depending on matrix Accessories RIDA® Sample Decolorant Reagents for the sample extraction of wine for histamine analysis ELISA microtiter plates RIDASCREEN® Histamin Competitive ELISA to quantify histamine in food; Detection limit: 0.1 - 100 mg/kg histamine depending on matrix Colorimetric assay for quantitative analysis RIDA®QUICK Histamin Colorimetric assay to quantify histamine in fish meal and fresh fish Detection limit: 0.1 - 100 mg/kg histamine depending on matrix 43 R-Biopharm AG – Product catalogue 2017 Allergen analysis of food and surfaces with sensitive test kits Even small traces of allergenic proteins in food can provoke allergic reactions in sensitive people. Therefore monitoring of cross-contamination in raw material and production lines as well as correct labeling of food products are an important part of quality control in the food industry. Surface and hygiene control Product testing Clean and controlled allergen production conditions are a prerequisite for allergenfree food products. Therefore swabs within production sites should be carried out regularly with Test strips from bioavid or RIDA®QUICK. No lab equipment is required and results from these rapid tests are available within 5 - 10 minutes. For food testing different analytical methods exist: ELISA, LFD and PCR. While an ELISA and LFD detect proteins; PCR detects DNA. These methods are complementary and can be used for confirmation of screening results. The unique multiplex real-time PCR allows the detection of 3 parameters plus internal amplification control in one run. 44 bioavid/RIDA®QUICK • • • • • On-site testing (swab test, food) Simple No lab equipment required Rapid yes/no decision Food after validation RIDASCREEN® ELISA • Quantitative results using certified calibration material (e.g. NIST CRM) • Simple sample preparation (20 min) and test procedure (3 x 10 min) • Possibility of using automates (ThunderBolt®, GEMINI) • Evaluation with the software RIDA®SOFT Win SureFood® PCR • Robust, stable target molecule (DNA) in highly processed food samples • Highly specific assay with minimum tendency to cross-reactions • One sample preparation using SureFood® PREP Advanced (S1053) for all parameters in 90 minutes • Customized solutions • Standardized handling and test procedure (1 - 2 hours) 45 R-Biopharm AG – Product catalogue 2017 Allergens Gliadin/Gluten Product Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN® Gliadin AOAC-OMA 2012.01“Final Action“ AOAC-RI 120601 AACCI 38-50.01 Codex Alimentarius Method (Type I) ICC Official R5 Mendez method: Sandwich ELISA to quantify prolamines from wheat, rye and barley in e.g. food declared as gluten-free; sample extraction with R7006 or R7016 (not contained in the kit); the kit is suitable for automation; Detection limit: 0.5 mg/kg gliadin or 1.0 mg/kg gluten 96 determinations Incubation time: 1 hr 30 min R7001 RIDASCREEN®FAST Gliadin R5 sandwich ELISA to quantify prolamines from wheat, rye, barley in e.g. food declared as gluten-free; sample extraction with R7006 or R7016 (not contained in the kit); Detection limit: 0.5 mg/kg gliadin or 1.0 mg/kg gluten 48 determination Incubation time: 30 min R7002 RIDASCREEN® Gliadin competitive (2nd generation) AACCI 38-55.01 AOAC-OMA 2105.05 R5 competitive ELISA to quantify potential toxic peptide sequences of prolamines from wheat, rye and barley in fermented and hydrolyzed food (e.g. beer, starch, starch syrup, malt extracts); sample preparation with an ethanolic solution; the standard material is a hydrolyzate (mixture of wheat, rye and barley); the results can be related to the limit values of the Codex Alimentarius Detection limit: 2.3 mg/kg gliadin or 4.6 mg/kg gluten 96 determinations Incubation time: 40 min R7021 ELISA – accessories Cocktail (patented) Developed by Prof. Mendez; officially recommended extraction buffer for all processed e.g. heat treated food samples in conjunction with R7001, R7002, R7003, R7004 105 ml R7006 Cocktail (patented) Corresponding to R7006 but larger bottle size 1000 ml R7016 RIDA® Extraction Solution (colorless) Alternative to the Cocktail (patented) (use only after extraction comparision with Cocktail): The extraction is faster (35 min compared to 1 h 50 min with the cocktail); it is used in conjunction with R7001, R7002, R7003 and R7004 105 ml R7098 Set of 3 processed Gliadin Assay Controls Three assay controls: 3 positive homogenized processed snack samples for the determination with sandwich ELISA; produced by Trilogy® Analytical Laboratories 3 x 1.5 g R7012 Lateral flow test strips RIDA®QUICK Gliadin AOAC-OMA 2015.16 AACCI The immunochromatographic test is based on the R5 antibody and detects prolamines from wheat, rye and barley; the Test strips can be used directly for swabs on surfaces or for analysis of e.g. gluten-free raw materials Detection limit: 1-2 μg gliadin/100 cm2 on surfaces, 2.2 mg/kg gliadin in raw materials, 3.1 mg/kg gliadin in processed food 25 Test strips in reclosable tube, 25 plastic pipettes, sample diluent (ready-to-use), 30 vials Incubation time: 5 min R7003 RIDA®QUICK Gliadin (single packaged) Corresponding to 7003, Test strips are single packaged and no plastic pipettes are included 25 Test strips single packed, sample diluent (ready-to-use), 30 vials Incubation time: 5 min R7004 RIDA®QUICK Gliadin (ready to swab) Corresponding to R7003, Test strips are single packaged, prefilled vials with ready-to-use sample buffer are included 25 Test strips single packed, 25 prefilled vials with ready-to-use buffer R7005 46 Allergens Gluten Product Description No. of tests/amount Art. No. Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Gluten Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation 100 reactions* S3106 100 reactions** S3206 48 determinations Incubation time: 30 min R7102 3x2g R7132 Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Gluten Detection limit: ≤ 0.4 mg/kg Quantification limit: 1 mg/kg depending on matrix and DNA preparation Soy ELISA microtiter plates RIDASCREEN®FAST Soya Sandwich ELISA to quantify traces of soy protein in native and processed food; the kit is suitable for automation; Detection limit: 0.24 mg/kg, the kit is suitable for automation ELISA – accessories Set of 3 processed Soya Assay Controls Three assay controls: 1 negative, 2 positive homogenized processed cookies; produced by Trilogy® Analytical Laboratories A SOY A SOY A YA PLE SOY SO YA SAM PLE SO YA A SAM PLE LE A SO SOY SAM MP LEA SOY YA LE PLE SA MP SOY SO YA MP SAM PLE SA SO YA SAM PLE SA A LE SO SOY SAM A MP LE SA SOY A MP LE YA PLE SA SOY SOYA SO YA SAMLE PLE SAMP SO YA SAMP SAMLEPLE SOYA LE SO SOYA SAM SAMP MP LE SA MPLE SAMP SA MPLE SOYA SA LE SOYA SAMP LE SOYA SAMP LE SAMP Lateral flow test strips RIDA®QUICK Soya Immunochromatographic test for the determination of soya on surfaces, also strongly heated soya samples were detected. It is recommended to use RIDA®QUICK Soya accessory pack (Z7103) for sample preparation. Detection limit: approx. 0.5 µg soya protein/100 cm² 25 dip sticks in reclosable tube, Conjugate, Extraction buffer, 30 Plastic tubes, 25 Tubes, 26 Swabs, 50 pipette tips Incubation time: 16 min R7103 Accessories for the use of the RIDA®QUICK Soya Test tube holder, floating rack, pipette Z7103 The buffer is used for food sample preparation 2 x 100 ml R7113 100 reactions* S3101 100 reactions** S3201 LE SAMP LE SAMP LE SAMP SOYA SOYA SOYA RIDA®QUICK Soya RIDA®QUICK Soya accessory pack RIDA®QUICK Soya Extraction buffer Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Soya Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Soya Detection limit: ≤ 0.4 mg/kg Quantification limit: 1 mg/kg depending on matrix and DNA preparation * Includes additional 100 reactions of Inhibition Control Mix (ICM). ** SureFood® QUANTARD Allergen 40 must be used for quantification. 47 R-Biopharm AG – Product catalogue 2017 Allergens Milk Produkt Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN®FAST Milk AOAC-RI 101501 Sandwich ELISA to quantify milk proteins (casein and ß-lactoglobulin) in food containing traces of milk components; the assay is calibrated to NIST SRM 1549a whole milk powder; the kit is suitable for automation; Detection limit: 0.7 mg/kg milk protein 48 determinations Incubation time: 30 min R4652 RIDASCREEN®FAST Casein (2nd generation) Sandwich ELISA to quantify casein in food containing traces of milk or casein/caseinates; the kit is suitable for automation; Detection limit: 0.12 mg/kg casein in ice cream, chocolate, beverages and 0.71 mg/kg casein for all other samples (extraction with Extractor 2) 48 determinations Incubation time: 30 min R4612 RIDASCREEN®FAST ß-Lactoglobulin Sandwich ELISA to quantify native and processed ß-lactoglobulin in food containing traces of milk or whey; the kit is suitable for automation; Detection limit: 0.19 mg/kg ß-lactoglobulin 48 determinations Incubation time: 30 min R4902 RIDASCREEN® ß-Lactoglobulin Competitive ELISA to quantify native and processed ß-lactoglobulin in hydrolyzed milk products (e.g. hypoallergenic baby food); Detection limit: 0.1 mg/kg ß-lactoglobulin 96 determinations Incubation time: 2 hrs 45 min R4901 30 ml concentrate, sufficient for 15 samples R4613 10 Test strips 25 Test strips Incubation time: 8 min BL613-10 BL613-25 ELISA – accessories RIDA® Extractor 2 The RIDA® Extractor 2 (R4613) is used for the sample preparation in • RIDASCREEN®FAST Milk (R4652) • RIDASCREEN®FAST Casein (R4612) • RIDASCREEN®FAST ß-Lactoglobulin (R4902). Lateral flow test strips bioavid Lateral Flow Milch/Milk Immunochromatographic tests for qualitative detection of milk; Detection limit: 1 mg/kg depending on matrix Reference material MoniQA Milk Reference Material Blank Gluten free cookie, milled, free from gluten, milk, egg, soy, peanut, tree-nuts, Negative Control and/or Matrix Material as basis for spiked samples Prepared and packaged by Trilogy Analytical Laboratories 1x5g MQA 082015 MoniQA Milk Reference Material SMP Dried skim milk powder, characterized, Positive Control and/ or use for spiked samples Prepared and packaged by Trilogy Analytical Laboratories 1x5g MQA 092014 MoniQA Milk Reference Material Low Dried skim milk powder incurred in gluten free cookies, milled, concentration approx. 10 ppm Prepared and packaged by Trilogy Analytical Laboratories 1x5g MQA 102016 MoniQA Milk Reference Material High Dried skim milk powder incurred in gluten free cookies, milled, concentration approx. 50 ppm Prepared and packaged by Trilogy Analytical Laboratories 1x5g MQA 082016 Set of 4 MoniQA Milk Reference controls Blank, SMP, High, Low Set includes each pouch of MQA 082015, MQA 092014, MQA 092016, MQA 082016 Prepared and packaged by Trilogy Analytical Laboratories 4x5g MQA 122016 48 Allergens Egg Produkt Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN® FAST Ei/Egg Protein Sandwich ELISA to quantify traces of whole egg powder in food; the assay is calibrated to NIST SRM 8445 whole egg powder; no cross-reactivity to chicken meat (raw and cooked); the kit is suitable for automation; Detection limit: 0.1 mg/kg whole egg powder, 0.03 mg/kg egg white protein 48 determinations Incubation time: 30 min R6402 RIDASCREEN®FAST Lysozym Sandwich ELISA to quantify traces of lysozyme in wine, cheese and sausage; Detection limit: 0.006 mg/kg lysozyme in wine, 0.016 mg/kg lysozyme in cheese and sausages 48 determinations Incubation time: 30 min R6452 10 Test strips 25 Test strips Incubation time: 8 min BL608-10 BL608-25 48 determinations Incubation time: 30 min R6901 10 Test strips 25 Test strips Incubation time: 10 min BL601-10 BL601-25 100 reactions* S3104 25 Test strips Incubation time: 10 min BL602-25 100 reactions* S3117 48 determinations Incubation time: 30 min R6872 10 Test strips 25 Test strips Incubation time: 10 min BL610-10 BL610-25 100 reactions* S3115 Lateral flow test strips bioavid Lateral Flow Ei/Egg Immunochromatographic tests for qualitative detection of egg; Detection limit: 1 mg/kg depending on matrix Nuts Almond ELISA microtiter plates RIDASCREEN®FAST Mandel/Almond Sandwich ELISA to quantify traces of almond in food; Detection limit: 1.2 mg/kg almond Lateral flow test strips bioavid Lateral Flow Almond Immunochromatographic tests for qualitative detection of almond; Detection limit: 1 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Almond Detection limit: ≤ 4 mg/kg; depending on matrix and DNA preparation Brazil nut Lateral flow test strips bioavid Lateral Flow Brazil nut Immunochromatographic tests for qualitative detection of brazil nut; Detection limit: 1 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Brazil nut Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation Cashew kernel ELISA microtiter plates RIDASCREEN®FAST Cashew Sandwich ELISA to quantify traces of cashew in food; Detection limit: 0.09 mg/kg cashew Lateral flow test strips bioavid Lateral Flow Cashew Kernel Immunochromatographic tests for qualitative detection of cashew kernel; Detection limit: 1 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Cashew Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation * Includes additional 100 reactions of Inhibition Control Mix (ICM). ** SureFood® QUANTARD Allergen 40 must be used for quantification. 49 R-Biopharm AG – Product catalogue 2017 Allergens Nuts Product Description Coconut Lateral flow test strips bioavid Lateral Flow Coconut Immunochromatographic tests for qualitative detection of coconut; Detection limit: 1 mg/kg depending on matrix Hazelnut ELISA microtiter plates RIDASCREEN®FAST Hazelnut DIN CEN/TS 15633-2 method Sandwich ELISA to quantify traces of hazelnut in food; Detection limit: 1.5 mg/kg hazelnut No. of tests/amount Art. No. 10 Test strips 25 Test strips Incubation time: 10 min BL600-10 BL600-25 48 determinations Incubation time: 30 min R6802 10 Test strips 25 Test strips Incubation time: 10 min BL604-10 BL604-25 100 reactions* S3102 100 reactions** S3202 48 determinations Incubation time: 30 min R6852 10 Test strips 25 Test strips Incubation time: 10 min BL605-10 BL605-25 100 reactions* S3116 100 reactions* S3118 48 determinations Incubation time: 30 min R6202 10 Test strips 25 Test strips Incubation time: 10 min BL606-10 BL606-25 100 reactions* S3103 100 reactions** S3203 Lateral flow test strips bioavid Lateral Flow Hazelnut Immunochromatographic tests for qualitative detection of hazelnut; Detection limit: 1 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Hazelnut Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Hazelnut Detection limit: ≤ 0.4 mg/kg Quantification limit: 1 mg/kg depending on matrix and DNA preparation Macadamia nut ELISA microtiter plates RIDASCREEN®FAST Macadamia Sandwich ELISA to quantify traces of macadamia in food; Detection limit: 0.38 mg/kg macadamia Lateral flow test strips bioavid Lateral Flow Macadamia nut Immunochromatographic tests for qualitative detection of macadamia nut; Detection limit: 1 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Macadamia nut Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation Pecan nut Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Pecan Detection limit: ≤ 4 mg/kg; depending on matrix Peanut ELISA microtiter plates RIDASCREEN®FAST Peanut AOAC-RI 030404 Sandwich ELISA to quantify traces of peanut in food; the assay is calibrated to NIST SRM 2387 peanut butter Detection limit: 1.3 mg/kg peanut Lateral flow test strips bioavid Lateral Flow Peanut Immunochromatographic tests for qualitative detection of peanut; Detection limit: 1 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Peanut Detection limit: ≤ 1 mg/kg; depending on matrix and DNA preparation Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Peanut Detection limit: ≤ 1 mg/kg Quantification limit: 4 mg/kg depending on matrix and DNA preparation * Includes additional 100 reactions of Inhibition Control Mix (ICM). ** SureFood® QUANTARD Allergen 40 must be used for quantification. 50 Allergens Nuts Product Description Pistachio Lateral flow test strips bioavid Lateral Flow Pistachio Immunochromatographic tests for qualitative detection of pistachio; Detection limit: 1 mg/kg depending on matrix No. of tests/amount Art. No. 10 Test strips 25 Test strips Incubation time: 10 min BL611-10 BL611-25 100 reactions* S3114 100 reactions** S3214 10 Test strips 25 Test strips Incubation time: 10 min BL607-10 BL607-25 100 reactions* S3107 100 reactions** S3207 48 determinations Incubation time: 30 min R7202 10 Test strips 25 Test strips Incubation time: 10 min BL609-10 BL609-25 100 reactions* S3108 100 reactions** S3208 Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Pistachio Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Pistachio Detection limit: ≤ 0.4 mg/kg Quantification limit: 1 mg/kg depending on matrix and DNA preparation Walnut Lateral flow test strips bioavid Lateral Flow Walnut Immunochromatographic tests for qualitative detection of walnut and pecan nut; Detection limit: 10 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Walnut Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Walnut Detection limit: ≤ 0.4 mg/kg Quantification limit: 1 mg/kg depending on matrix and DNA preparation Oil plants ELISA microtiter plates RIDASCREEN®FAST Sesame Sandwich ELISA to quantify traces of sesame in food; Detection limit: 0.2 mg/kg sesame Lateral flow test strips bioavid Lateral Flow Sesam/Sesame Immunochromatographic tests for qualitative detection of sesame Detection limit: 1 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Sesame Detection limit: ≤ 0.4 mg/kg depending on matrix and DNA preparation Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Sesame Detection limit: ≤ 0.4 mg/kg Quantification limit: 1 mg/kg depending on matrix and DNA preparation * Includes additional 100 reactions of Inhibition Control Mix (ICM). ** SureFood® QUANTARD Allergen 40 must be used for quantification. 51 R-Biopharm AG – Product catalogue 2017 Allergens Fish/Crustacean/Seafood Product Description No. of tests/amount Art. No. ELISA microtiter plates RIDASCREEN®FAST Crustacean (2nd generation) Sandwich ELISA to quantify traces of crustacean in food; Detection limit: 2 mg/kg crustacean 48 determinations Incubation time: 30 min R7312 10 Test strips 25 Test strips Incubation time: 10 min BL616-10 BL616-25 Lateral flow test strips bioavid Lateral Flow Crustacean Immunochromatographic tests for qualitative detection of crustacean; Detection limit: 10 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Crustaceans Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation 100 reactions* S3112 SureFood® ALLERGEN ID Fish Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation 100 reactions* S3110 SureFood® ALLERGEN ID Molluscs Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation 100 reactions* S3113 100 reactions* S3105 100 reactions** S3205 48 determinations Incubation time: 30 min R6102 100 reactions* S3111 100 reactions** S3211 48 determinations Incubation time: 30 min R6152 10 Test strips 25 Test strips Incubation time: 10 min BL603-10 BL603-25 100 reactions* S3109 Various Celery Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Celery Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Celery Detection limit: ≤ 0.4 mg/kg Quantification limit: 1 mg/kg depending on matrix and DNA preparation Lupin ELISA microtiter plates RIDASCREEN®FAST Lupine Sandwich ELISA to quantify traces of lupin in food Detection limit: 0.7 mg/kg lupin protein Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Lupin Detection limit: ≤ 0.4 mg/kg; depending on matrix and DNA preparation Real-time PCR – quantitative DNA detection SureFood® ALLERGEN QUANT Lupin Detection limit: ≤ 0.4 mg/kg Quantification limit: 2.6 mg/kg depending on matrix and DNA preparation Mustard ELISA microtiter plates RIDASCREEN®FAST Senf/Mustard Sandwich ELISA to quantify traces of mustard in food; the assay detects yellow, white, brown and black mustard Detection limit: 0.22 mg/kg mustard powder Lateral flow test strips bioavid Lateral Flow Senf/Mustard Immunochromatographic tests for qualitative detection of mustard; Detection limit: 1 mg/kg depending on matrix Real-time PCR – qualitative DNA detection SureFood® ALLERGEN ID Mustard Detection limit: ≤ 0.4 mg/kg depending on matrix and DNA preparation * Includes additional 100 reactions of Inhibition Control Mix (ICM). ** SureFood® QUANTARD Allergen 40 must be used for quantification. 52 Allergens Real-time PCR – Multiplex Product Description Multiplex Screening Real-time PCR – qualitative DNA detection No. of tests/amount Art. No. SureFood® ALLERGEN ID 4plex Peanut/Hazelnut/Walnut + IAC Detection limit: ≤ 1 mg/kg; depending on matrix and DNA preparation 100 reactions S3402 SureFood® ALLERGEN ID 4plex Soya/Celery/Mustard + IAC Detection limit ≤ 0.4 mg/kg; depending on matrix and DNA preparation 100 reactions S3401 50 preparations S1053 2 grams S3301 Accessories Real-time PCR DNA preparation SureFood® PREP Advanced For highly processed matrices (food and feed) Laboratory reference material for quantification SureFood® QUANTARD Allergen 40 Corn flour contains 12 potential allergens in food except sulphite and lactose with concentration of 40 mg/kg. The material has been developed for PCR quantification of allergens in food Lateral Flow Tests strips bioavid Wischtest Kit/Swabbing Kit Swabbing kit with wood swabs for sampling of allergen residues on surface (e.g. production lines) for bioavid lateral flow kits 25 swabs, vials, pipettes, 10 ml buffer concentrate BS800-25 bioavid Wischtest Kit/Swabbing Kit (Plastic) Swabbing kit with single packed plastic swabs for sampling of allergen residues on surface (e.g. production lines) for bioavid Test strips 26 swabs in two single packaged plastic bags, vials, pipettes, 10 ml buffer concentrate BS801-25 bioavid Absorptionspuffer/Absorbent Buffer Buffer for preparation of polyphenol containing and strongly colored samples (e.g. coffee, red wine) for bioavid lateral flow kits 25 vials (9 ml buffer each) BS810-25 bioavid Probenpuffer/Sample Buffer The buffer is particularly suitable for preparation of difficult samples (e.g. ketchup, mayonnaise, flour) for bioavid Test strips 100 ml BS815-100 Approx. 1 week processing time on request Service by bioavid Laboratory service Service for the validation of difficult food matrices 53 R-Biopharm AG – Product catalogue 2017 GMO analysis in food and feed Commercially available genetically modified organisms (GMO) are usually transgenic plants in which DNA from foreign species were artificially implemented. These DNA sequences, mostly for herbicide and/or insect resistance are enveloped in a frame of viral or bacterial DNA sequences which serves as promoters or terminators. Different international and national legislations and labelling regulations require a multi-stage analysis, for which real-time PCR is the method of choice. In October 2015, the European Network of GMO Laboratories (ENGL) defined minimum performance requirements, which are fulfilled by the SureFood® kits. 1. The presence of GMOs can be screened by identifying the genetic sequence elements 35S, NOS or FMV. Further genetic elements may be expected in the future. 35S positive results should be confirmed for absence of natural contamination with the cauliflower mosaic virus using the CaMV detection kit. Furthermore, the efficiency of the DNA preparation should be confirmed using plant DNA, when analysing a new matrix. 54 2. For GMO positive samples the identification of the GMO event is of main interest, to classify the food product as approved or illegal GMO. In Europe the legislation EC 1829/2003 and 1830/2003 describes the relevant regulations. Non-approved GMO products are not allowed to enter or to be produced or processed in Europe. A zero tolerance strategy is in force for Europe, while for feed samples a technical threshold of 0.1 % has been established (EC 618/2011). Food products with a content of > 0.9 % approved GMO per matrix must be labelled. 3. For approved GMOs in food samples quantification in the relevant range of approximately 0.9 % is of main interest. The GMO content in DNA copy numbers can be quantified relative to the plant matrix and the results will be given in percent. SureFood® PREP Basic/Advanced • Efficient, streamlined DNA sample preparation from food and feed matrices • Highly purified DNA SureFood® GMO SCREEN • Multiplex assay for 35S/NOS/FMV + IAC, BAR/NPTII/PAT/CTP2:CP4 EPSPS, Corn/Soya/Canola/Cotton • Single assays for vectors SureFood® GMO QUANT • • • • Identification and quantification Robust detection system Wide product range Suitable for most available real-time thermocyclers 55 R-Biopharm AG – Product catalogue 2017 GMO DNA preparation Product Description No. of tests/amount Art. No. SureFood® PREP Basic DNA preparation of food and feed 100 preparations S1052 SureFood® PREP Advanced DNA preparation of highly processed food and feed 50 preparations S1053 SureFast® Animal+Plant Control Extraction control for plant or animal matrix 100 reactions F4053 SureFood® GMO Plant PLUS Detection limit: 5 DNA copies depending on matrix and DNA preparation 100 reactions S2049 SureFood® GMO Plant Complementary kit to S2026 and S2126 Detection limit: 5 DNA copies depending on matrix and DNA preparation 100 reactions S2056 SureFood® GMO SCREEN CaMV Detection limit: 5 DNA copies depending on matrix and DNA preparation 100 reactions S2027 SureFood® GMO SCREEN P35S:BAR Rice Detection limit: 0.01 % depending on matrix and DNA preparation 2 x 50 reactions S2022 SureFood® GMO SCREEN 35S + NOS + FMV Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions* S2026 SureFood® GMO SCREEN 4plex 35S/NOS/FMV + IAC Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2126 SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton Detection limit: 4 ppm depending on matrix and DNA preparation 100 reactions S2156 SureFood® GMO Plant 4plex Corn/Soya/Canola + IAC Detection limit: 4 ppm depending on matrix and DNA preparation 100 reactions S2158 SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2127 Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2062 SureFood® GMO ID MIR162 Corn Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2035 SureFood® GMO ID MON863 Corn Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2037 SureFood® GMO ID LibertyLink601 Rice Detection limit: 0.01 % depending on matrix and DNA preparation 2 x 50 reactions S2023 SureFood® GMO ID Bt63 Rice Detection limit: 0.01 % depending on matrix and DNA preparation 2 x 50 reactions S2024 DNA preparation Screening Screening Multiplex screening Real-time PCR – qualitative DNA detection Canola SureFood® GMO ID MS8 Canola Corn Rice * Includes additional 100 reactions of Inhibition Control Mix (ICM); 100 reactions for each parameter. 56 GMO Real-time PCR – qualitative DNA detection Product Description No. of tests/amount Art. No. SureFood® GMO ID Roundup Ready Soya Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2030 SureFood® GMO ID RR2Y Soya Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2034 SureFood® GMO ID A2704-12 Soya Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2057 Detection limit: 0.01 % depending on matrix and DNA preparation 100 reactions S2161 Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2061 SureFood® GMO QUANT Bt176 Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2015 SureFood® GMO QUANT Bt11 Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2016 SureFood® GMO QUANT T25 Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2017 SureFood® GMO QUANT MON810 Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2019 SureFood® GMO QUANT 35S Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2020 SureFood® GMO QUANT NK603 Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2050 SureFood® GMO QUANT MON863 Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2051 SureFood® GMO QUANT MIR162 Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2135 SureFood® GMO QUANT GA21 Corn Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2054 SureFood® GMO QUANT Roundup Ready Soya Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2014 SureFood® GMO QUANT 35S Soya Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2028 SureFood® GMO QUANT RR2Y Soya Limit of quantification: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S2029 Soya Multiplex real-time PCR SureFood® GMO ID 4plex Soya I MON87708, CV127, DP305423, MON87701, MON87769 Real-time PCR – quantitative DNA detection Canola SureFood® GMO QUANT GT73 Canola Corn Soya ** 1 x 50 reactions for the detection of the reference gene. 57 R-Biopharm AG – Product catalogue 2017 Identification of animal species/risk material/BSE Due to the increasing complexity of meat supply chains, and prevalent product falsifications, species identification testing has become a cornerstone of food quality assurance and fraud prevention. Real-time PCR delivers robust, reliable results even from processed food and feed samples. Animal species detection The aspects of animal species detection might be categorized into three application groups: • Product falsification Product falsification with cheaper undeclared meat might be identified qualitatively and quantitatively using the ANIMAL ID and QUANT kits. • Species detection In some cases, especially for religious aspects such as kosher or halal with a zero tolerance strategy, highly sensitive qualitative detection is required. The ANIMAL ID Pork SENS PLUS kit enables an extremely sensitive detection. • Fish species detection According to the EC 1379/2013 regulation fish products must be labelled with the common trade name and the scientific name. Fish ID real-time kits are available for the most important fish species. 58 • Feed Due to the ending of the BSE crisis, it might be expected that meat and bone meal (MBM) will be used to feed animals again. However, feeding to ruminants should be avoided. Due to its stability, DNA is an excellent marker for animal identification. Real-time PCR can be used even for processed food and feed samples, with the exception of some highly processed products such as gelatin. The new product line with Internal Amplification and Animal Control (IAAC) has higher sensitivity and includes an amplification and extraction control. SureFood® PREP Basic SureFood® ANIMAL ID 59 R-Biopharm AG – Product catalogue 2017 Animal species indentification/risk material/BSE Real-time PCR – qualitative DNA detection Product Description No. of tests/amount Art. No. DNA preparation SureFood® PREP Basic DNA preparation of food and feed 100 preparations S1052 SureFast® Animal+Plant Control Extraction control for plant or animal matrix 100 reactions F4053 SureFood® ANIMAL ID 4plex Beef/Sheep/Goat + IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6121 SureFood® ANIMAL ID 4plex Pork/Chicken/Turkey+IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6123 SureFood® ANIMAL ID Beef IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6113 SureFood® ANIMAL ID Horse IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6118 SureFood® ANIMAL ID Horse & Donkey IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6119 SureFood® ANIMAL ID Pork SENS PLUS Detection limit: ≤ 0.0005% depending on matrix and DNA preparation 100 reactions S6017 SureFood® ANIMAL ID Pork IAAC* Detection limit: 0.5 % depending on matrix and DNA preparation 100 reactions S6114 SureFood® ANIMAL ID Waterbuffalo IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6117 SureFood® ANIMAL ID Chicken IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6115 SureFood® ANIMAL ID Turkey IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6116 Detection limit: 0.5 % depending on matrix and DNA preparation 100 reactions S6112 Multiplex screening Farm animals Poultry Other species SureFood® ANIMAL ID Cat & Dog IAAC* * IAAC = Internal Amplification and Animal Control. The IAAC kits require a 2-channel real-time thermocycler (FAM und VIC/HEX). These kits have been validated on Bio-Rad CFX96, Qiagen Rotor-Gene, Roche LC480 and Agilent Mx3005P. Exception: S6119 requires a 3-channel and S6121 a 4-channel device. 60 Animal species indentification/risk material/BSE Real-time PCR – quantitative DNA detection Product Description No. of tests/amount Art. No. SureFood® ANIMAL ID Rabbit IAAC* Detection limit: 0.1 % depending on matrix and DNA preparation 100 reactions S6120 SureFood® ANIMAL QUANT Beef Detection limit: 0.04 % depending on matrix and DNA preparation 2 x 50 reactions** S1010 SureFood® ANIMAL QUANT Equus Detection of horse, donkey and zebra Detection limit: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S1016 SureFood® ANIMAL QUANT Sheep Detection limit: 0.1 % depending on matrix and DNA preparation 2 x 50 Reaktionen** S1017 SureFood® ANIMAL QUANT Pork Detection limit: 0.04 % depending on matrix and DNA preparation 2 x 50 reactions** S1011 Farm animals Poultry ** 1 x 50 reactions for the detection of the reference gene. 61 R-Biopharm AG – Product catalogue 2017 Animal species indentification/risk material/BSE SureFood® FISH ID Product Description No. of tests/amount Art. No. SureFood® ANIMAL QUANT Chicken Detection limit: 0.1 % depending on matrix and DNA preparation 2 x 50 reactions** S1014 SureFood® FISH ID 3plex Halibut IAAC* Differentiation of white (Hippoglossus hippoglossus) and black halibut (Reinhardtius hippoglossoides); Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6201 SureFood® FISH ID Oncorhynchus tshawytscha IAAC* Detection of chinook salmon; Detection limit: 2 % depending on matrix and DNA preparation 50 reactions S6301 SureFood® FISH ID Oncorhynchus mykiss IAAC* Detection of rainbow trout; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6302 SureFood® FISH ID Oncorhynchus gorbuscha IAAC* Detection of humpback salmon; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6303 SureFood® FISH ID Oncorhynchus nerka IAAC* Detection of red salmon; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6304 SureFood® FISH ID Salmo trutta IAAC* Detection of trout; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6305 SureFood® FISH ID Salmo salar IAAC* Detection of atlantic salmon; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6306 SureFood® FISH ID Gadus macrocephalus IAAC* Detection of pacific cod; Detection limit: 2 % depending on matrix and DNA preparation 50 reactions S6308 SureFood® FISH ID Gadus morhua IAAC* Detection of atlantic cod; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6310 SureFood® FISH ID Pollachius virens IAAC* Detection of pollock/saithe; Detection limit: 2 % depending on matrix and DNA preparation 50 reactions S6309 SureFood® FISH ID Merlangius merlangus IAAC* Detection of whiting; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6312 SureFood® FISH ID Gadus chalcogrammus IAAC* Detection of Alaska pollock; Detection limit: 5 % depending on matrix and DNA preparation 50 reactions S6313 SureFood® FISH ID Merluccius merluccius IAAC* Detection of european hake; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6311 Fish * IAAC = Internal Amplification and Animal Control. The IAAC kits require a 2-channel real-time thermocycler (FAM und VIC/HEX). These kits have been validated on Bio-Rad CFX96, Qiagen Rotor-Gene, Roche LC480 and Agilent Mx3005P. Exception: S6119 requires a 3-channel and S6121 a 4-channel device. 62 Animal species indentification/risk material/BSE Risk material Product Description No. of tests/amount Art. No. SureFood® FISH ID Melanogrammus aeglefinus IAAC* Detection of haddock; Detection limit: 1 % depending on matrix and DNA preparation 50 reactions S6307 Enzyme immunoassay for quantitative analysis of risk material (CNS) in processed meat and meat products Detection limit: < 0.2 % for CNS tissue 96 determinations Incubation time: 1 hr R6701 Enzyme immunoassay for qualitative analysis of risk material (CNS) in raw meat, meat products and on contaminated surfaces Detection limit: < 0.1 % for CNS tissue 96 determinations Incubation time: 15 min R6703 ELISA microtiter plates RIDASCREEN® Risk Material BSE RIDASCREEN® Risk Material 10/5 BSE/antibody RIDA® mAb L42 Monoclonal antibody for the detection of prion-protein with immunohistochemistry (IHC) and immunoblot 23 µg R8005 RIDA® mAb P4 Monoclonal antibody for the detection of prion-protein with immunohistochemistry (IHC) and immunoblot 1 mg R8007 RIDA® mAb P4 Monoclonal antibody for the detection of prion-protein with immunohistochemistry (IHC) and immunoblot 0.1 mg R8008 63 R-Biopharm AG – Product catalogue 2017 Analysis for microbiological food safety Rapid test formats for reliable microbiological analysis in food and plants for highly specific, sensitive and fast test combinations for use with a wide range of applications. Product testing All kinds of commodities are potentially at risk of contamination by spoiling organisms and pathogens. Therefore, R-Biopharm offers reliable kits for the analysis of meat and meat-products, dairy products, egg and egg-products, vegetable, fruits, herbs and spices, beverages, cereals and cerealproducts as well as prepared meals. Well-established methods for on-site testing are classic microbiological testing, highly specific detection with real-time PCR, or confirmation of bacterial toxins by ELISA-tests. 64 Production surrounding area and condition Quality and safety standards are considered when minimizing the risk of product contamination. Important characteristics for tests used in efficient hygiene and cleaning control are: • High sensitivity • Rapidness • Repeatability Reliability of results is important for immediate and long-term decisions. Bacterial toxins and pathogens RIDASCREEN® ELISA • Detection of bacterial toxins • Detection of pathogens Compact Dry Dry nutrient media for detection of pathogens SureFast® realt-time PCR • Real-time PCR for screening und species identification • Effective DNA/RNA-extraction • Multiplex real-time kits Bacteria, viruses, contaminants and spoilers SureFast® real-time PCR • For screening und species identification • Effective DNA/RNA-extraction • Multiplex real-time kits Compact Dry Dry nutrient media for enumeration of microorganisms Hygiene & cleaning Compact Dry with wet swab systems, RIDA®STAMP Nutrient media for detection of microorganisms Lumitester PD-30 with LuciPac Pen • Sensitive AMP/ATP detection • Automatic alinement of measured data • Software based evaluation RIDA®CHECK • Detection of protein residues • Colorimetric test for rapid cleaning control 65 R-Biopharm AG – Product catalogue 2017 Microbiology/hygiene Culture medium systems for colony counting and pathogen detection in food or surface samples Product Description Compact Dry Nutrient pads No. of tests/amount Art. No. Compact Dry AQ Test plate with nutrient pad for quantitative detection of heterotrophic water bacteria 100 determinations 40 determinations HS9541 HS9542 Compact Dry CC Test plate with nutrient pad for detection of total aerobic count in teaproducts 100 determinations 40 determinations HS7311 HS7312 Compact Dry CF MicroVal MV0806-003L; NordVal 35; AOAC-RI 110401 Test plate with nutrient pad for quantitative detection of coliforms 100 determinations 40 determinations HS8791 HS8792 Compact Dry EC MicroVal MV0806-004LR; NordVal 36; AOAC-RI 110402 Test plate with nutrient pad for quantitative detection of E. coli and coliforms 100 determinations 40 determinations HS8781 HS8782 Compact Dry ETB MicroVal MV0806-002LR; NordVal 34 Test plate with nutrient pad for quantitative detection of Enterobacteriaceae 100 determinations 40 determinations HS9431 HS9432 Compact Dry ETC NordVal 47 Test plate with nutrient pad for quantitative detection of Enterococci 100 determinations 40 determinations HS9461 HS9462 Compact Dry LS Test plate with nutrient pad for quantitative detection of Listeria spp. 100 determinations 40 determinations HS8811 HS8812 Compact Dry PA Test plate with nutrient pad for quantitative detection of Pseudomonas aeruginosa 100 determinations 40 determinations HS9491 HS9492 Compact Dry SL Test plate with nutrient pad for detection of Salmonella 100 determinations 40 determinations HS9401 HS9402 Compact Dry TC MicroVal RQA2007LR01; NordVal 33; AOAC-RI 10404 Test plate with nutrient pad for detection of total aerobic count 100 determinations 40 determinations HS8771 HS8772 Compact Dry VP Test plate with nutrient pad for quantitative detection of Vibrio parahaemolyticus and Vibrio spp. 100 determinations 40 determinations HS8821 HS8822 Compact Dry YM MicroVal RQA2008LR10; NordVal 43; AOAC-RI 100401 Test plate with nutrient pad for quantitative detection of yeast and mold 100 determinations 40 determinations HS8801 HS8802 Compact Dry YMR (Approvals in progress) Test plate with nutrient pad for rapid quantitative detection of yeast and mold in 48 - 72 h 100 determinations 40 determinations HS9801 HS9802 Compact Dry X-BC MicroVal 2011LR41; NordVal 45 Test plate with nutrient pad for quantitative detection of Bacillus cereus 100 determinations 40 determinations HS9721 HS9722 Compact Dry X-SA MicroVal 2008LR14; NordVal 42; AOAC-RI 81001 Test plate with nutrient pad for quantitative detection of Staphylococcus aureus 100 determinations 40 determinations HS9621 HS9622 Accessories RIDA® 0.9 % NaCl, sterile 1 ml sterile sodium chloride solution 150 pieces (1 ml each) Z0301 Promedia ST-25 Sampling device (sterile swab in 10 ml sterile PBS buffer) 10 pieces Z0302 Compact Dry Swab Sampling set (sterile swabs in 1 ml PBS/peptone buffer) 40 pieces ZCS1002953 Dilution Rack-PBS Dilution set for preparation of 10-fold dilution series (9 ml PBS buffer per well) - sterile 128 pieces ZDP1000888 Dilution Rack-MRD Dilution set for preparation of 10-fold dilution series (9 ml MRD buffer per well) - sterile 128 pieces ZDM1000889 Opener for Dilution Rack For sterile opening of Dilution Rack 1 piece ZOP1000887 Frame - 100 cm Frame for definition of 100 cm for swab sampling 5 pieces ZFR1600000 2 66 2 Microbiology/hygiene Culture medium systems for surface specimen/hygiene monitoring Product Description No. of tests/amount Art. No. RIDA®STAMP Coliform Agar stamp plate for surface monitoring of Coliforms on solid foods and environmental surfaces 50 determinations 25 determinations HS0411 HS0412 RIDA®STAMP ECC Agar stamp plate for surface monitoring of E. coli & Coliforms on solid foods and environmental surfaces 50 determinations 25 determinations HS0431 HS0432 RIDA®STAMP Pseudomonas Agar stamp plate for surface monitoring of Pseudomonas spp. on solid foods and environmental surfaces 50 determinations 25 determinations HS2011 HS2012 RIDA®STAMP Salmonella Agar stamp plate for surface monitoring of Salmonella on solid foods and environmental surfaces 25 determinations HS0392 RIDA®STAMP S. aureus Agar stamp plate for surface monitoring of Staphylococcus aureus on solid foods and environmental surfaces 25 determinations HS0462 RIDA®STAMP Total Agar stamp plate for surface monitoring of total count on solid foods and environmental surfaces 50 determinations 25 determinations HS0291 HS0292 RIDA®STAMP Total Desi Agar stamp plate for surface monitoring of total count in case of presence of desinfectants on solid foods and environmental surfaces 50 determinations 25 determinations HS1831 HS1832 RIDA®STAMP YM-P Agar stamp plate for surface monitoring of fungi on solid foods and environmental surfaces 50 determinations 25 determinations HS0371 HS0372 67 R-Biopharm AG – Product catalogue 2017 Microbiology/hygiene Pathogens & bacterial toxins Product Description No. of tests/amount Art. No. DNA preparation SureFast® PREP Bacteria Preparation of bacteria DNA 100 preparations F1021 SureFast® Speed PREP Preparation of bacteria- and parasites-DNA from enrichment cultures and tissue samples 100 preparations F1054 Bacillus cereus Qualitative real-time PCR SureFast® Bacillus cereus group PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5126 SureFast® Emetic Bacillus cereus PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5127 Campylobacter ELISA microtiter plates RIDASCREEN® Campylobacter Enzyme immunoassay for the determination of Campylobacter in food 48 determinations R4204 100 reactions F5112 Qualitative real-time PCR SureFast® Campylobacter PLUS (C. jejuni, C. lari, C. coli) Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment Clostridium Qualitative real-time PCR SureFast® Clostridium botulinum Screening PLUS Qualitative DNA detection Detection of C. botulinum toxin groups A, B, E, F Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5110 SureFast® Clostridium perfringens PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5123 Cronobacter Qualitative real-time PCR SureFast® Cronobacter PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5114 SureFast® Cronobacter sakazakii PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5115 E.coli ELISA microtiter plates RIDASCREEN® Verotoxin Enzyme immunoassay for the detection of pathogenic E. coli (indirectly via verotoxins 1 and 2) 96 determinations Incubation time: 1 hr 45 min R5701 100 tubes 25 tubes Z1000 Z1003 Accessories Enrichment broth mTSB-bouillon with Mytomycin C for the enrichment of verotoxin (shigatoxin)-producing E. coli bacteria Qualitative real-time PCR SureFast® Escherichia coli PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5157 SureFast® Escherichia coli eae gene Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5104 SureFast® EHEC/EPEC 4plex Qualitative DNA detection of virulence gene stx1, stx2, eae, ipaH (E. coli/Shigella differenciation) 100 reactions F5128 SureFast® STEC Screening PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5105 Listeria ELISA microtiter plates RIDASCREEN® Listeria Enzyme immunoassay for the determination of Listeria in food 96 determinations R4202 Qualitative real-time PCR SureFast® Listeria Screening PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5117 SureFast® Listeria monocytogenes PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5113 68 Microbiology/hygiene Pathogens & bacterial toxins Product Description Pseudomonas Qualitative real-time PCR SureFast® Pseudomonas aeruginosa PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, Salmonella ELISA RIDASCREEN® Salmonella AFNOR RBP 31/01-06/08 Enzyme immunoassay for the determination of Salmonella in food, feed and environmental samples No. of tests/amount Art. No. 100 reactions F5503 96 determinations R4201 100 preparations F1007 100 reactions F5111 DNA preparation SureFast® PREP Salmonella AOAC-RI 041103 DNA preparation of Salmonella Qualitative real-time PCR SureFast® Salmonella PLUS AOAC-RI 041103 Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment Qualitative real-time PCR and DNA preparation SureFast® Salmonella ONE Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment Kit includes DNA preparation 100 reactions / 100 preparations F5211 SureFast® Salmonella Serotype 3plex Qualitative DNA detection of S. typhimurium and S. enteritidis Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5159 Staphylococcus ELISA microtiter plates RIDASCREEN® SET A, B, C, D, E Enzyme immunoassay for identification of staphylococcus enterotoxins A, B, C, D and E in food and bacterial cultures Detection limit: 0.25 ng/ml toxin (0.375 ng/g) 12 determinations Incubation time: 2 hrs 45 min R4101 RIDASCREEN® SET Total Enzyme immunoassay for combined detection of staphylococcus enterotoxins (A - E) in food and bacterial cultures Detection limit: 0.25 ng/ml toxin (0.375 ng/g) 96 determinations 48 determinations Incubation time: 2 hrs 45 min R4105 R4106 100 reactions F5116 Qualitative real-time PCR SureFast® Staphylococcus aureus PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment Vibrio Qualitative real-time PCR SureFast® Vibrio Screening PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5118 SureFast® Vibrio 4 plex (V. cholerae, V. parahaemolyticus, V. vulnificus +IAC) Qualitative DNA detection Detection limit: ≤ 5 DNA copies, 1 cfu after enrichment 100 reactions F5161 Yersinia Qualitative real-time PCR SureFast® Yersinia enterocolitica PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies 100 reactions F5124 Histomonas (parasites) Qualitative real-time PCR SureFast® Histomonas meleagridis PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies 100 reactions F5158 100 reactions / 100 preparations F5213 Qualitative real-time PCR and DNA preparation SureFast® Histomonas meleagridis ONE Qualitative DNA detection Detection limit: ≤ 5 DNA copies, Kit includes a solution for DNA-extraction 69 R-Biopharm AG – Product catalogue 2017 Microbiology/hygiene Viruses Product Description No. of tests/amount Art. No. DNA/RNA preparation SureFast® PREP DNA/RNA Virus DNA/RNA preparation of viruses 100 preparations F1051 real-time reverse transcriptase PCR (qualitative detection) SureFast® Norovirus PLUS Qualitative detection of Norovirus genogroup I and II Detection limit: ≤ 50 RNA copies 100 reactions F7001 SureFast® Norovirus I & II 3plex Qualitative detection and differentiation of Norovirus genotype I and II Detection limit: ≤ 50 RNA copies 100 reactions F7140 SureFast® Norovirus/Hepatitis A 3plex Qualitative detection of Norovirus and Hepatitis A Detection limit: ≤ 50 RNA copies 100 reactions F7124 SureFast® Hepatitis A PLUS Qualitative detection of Hepatitis A Detection limit: ≤ 50 RNA copies 100 reactions F7125 SureFast®Influenza A PLUS Qualitative detection of influenza virus A Detection limit: ≤ 25 RNA copies 100 reactions F7103 SureFast®Influenza A H5/H7/H9 4plex Qualitative detection and differentiation of influenza virus A, H5, H7 and H9 Detection limit: ≤ 25 RNA copies 100 reactions F7139 100 preparations F1023 Water analysis DNA preparation SureFast® PREP Aqua DNA preparation of bacterial cells from water samples Legionella Qualitative real-time PCR SureFast® Legionella Screen PLUS Qualitative DNA detection of Legionella spp. 100 reactions F5502 SureFast® Legionella pneumophila PLUS Qualitative DNA detection of Legionella pneumophila 100 reactions F5501 SureFast® Legionella 3plex Qualitative DNA detection of Legionella spp. and Legionella pneumophila Detection limit: ≤ 5 DNA copies 100 reactions F5505 Qualitative real-time PCR SureFast® Parasitic Water Panel 4plex Qualitative DNA detection Detection limit: ≤ 5 DNA copies 100 reactions F5506 SureFast® Enterobacteriaceae Screening PLUS Qualitative DNA detection Detection limit: ≤ 5 DNA copies 100 reactions F5507 SureFast® Pseudomonas aeruginosa PLUS Qualitative DNA detection of Pseudomonas aeruginosa, Detection limit: ≤ 5 DNA copies 100 reactions F5503 AMP/ATP detection Bioluminescence LuciPac® Pen AQUA Test system for hygiene control in liquid samples (based on detection of ATP/AMP) Reaction tubes with integrated sample stick for use with Lumitester PD-30 100 reactions ZLA1002672 20 reactions ZLPP1002673 Accessories RIDA® Clean Extract Sample preparation kit for lubricants and paints to be used together with LuciPac® Pen AQUA * Find more products for microbiological water analysis on page 66 under “Culture Medium Systems for Colony Counting and Pathogen Detection”. 70 Microbiology/hygiene Beverage analysis Product Description Juice DNA preparation GEN-IAL® Simplex® Easy Spin DNA kit Alicyclobacillus DNA extraction from fruit or vegetable juices or concentrates No. of tests/amount Art. No. 50 preparations SES 0050 DNA Screening of Alicyclobacillus ssp., A. acidocaldarius and A. acidoterrestris in fruit juices or concentrates 50 reactions TPABM 0050 GEN-IAL® Alicyclobacillus spp. Specific DNA detection of Alicyclobacillus 50 reactions TPAB 0050 Wine DNA preparation 100 preparations SEW 0100 Qualitative multiplex real-time PCR GEN-IAL® Alicyclobacillus multiplex TaqMan™ GEN-IAL® Simplex® Easy Wine kit DNA preparation of wine samples Qualitative multiplex real-time PCR GEN-IAL® First-Wine PCR Screening TaqMan™ DNA screening and differentiation of wine spoilage bacteria and yeasts: Lactobacillus; Pediococcus; Oenococcus oeni; acetic acid bacteria; yeast 50 reactions TPWS 0050 GEN-IAL® First-Wine PCR Screening TaqMan™ DNA Screening of wine spoilage bacteria: Lactobacillus; Pediococcus; Oenococcus oeni; acetic acid bacteria 50 reactions TPWSOH 0050 Qualitative real-time PCR GEN-IAL® First-Oenococcus Oeni Specific DNA detection of Oenococcus oeni 50 reactions TPOE 0050 GEN-IAL® First-Wine Screening Biogene Amine Specific DNA detection of bacteria forming biogenic amines 50 reactions BAM 0050 Wine/beer Qualitative real-time PCR GEN-IAL® Acetic acid bacteria TaqMan™ Specific DNA detection of acetic acid bacteria 50 reactions TPA 0050 GEN-IAL® QuickGEN* Acetic acid bacteria TaqMan™ Specific DNA detection of acetic acid bacteria 50 reactions QTPA 0050 GEN-IAL® Dekkera bruxellensis TaqMan™ FH Specific DNA detection of Dekkera bruxellensis (FAM HEX) 50 reactions TPYDB 0050 FH GEN-IAL® Dekkera bruxellensis TaqMan™ FR Specific DNA detection of Dekkera bruxellensis (FAM ROX) 50 reactions TPYDB 0050 FR GEN-IAL® Dekkera bruxellensis TaqMan™ Spartan DX-12 Specific DNA detection of Dekkera bruxellensis 50 reactions TPYDB 0050 SP GEN-IAL® Dekkera bruxellensis TaqMan™ MyGo Pro Specific DNA detection of Dekkera bruxellensis 50 reactions QTPYDB 0050 MG * QuickGEN kits deliver a rapid, easy one-step DNA lysis and amplification method. Pre-enriched samples as well as centrifuged or filtrated samples may be used with CSE 0100 or FSE 0100 and subsequent QuickGEN detection kits. 71 R-Biopharm AG – Product catalogue 2017 Microbiology/hygiene Beverage analysis Product Description Beer DNA preparation No. of tests/amount Art. No. GEN-IAL® Simplex® Easy DNA kit DNA preparation of beverage samples 100 preparations SE 0100 GEN-IAL® QuickGEN* Sample preparation filtration DNA preparation of beverage samples, Filtration 100 preparations FSE 0100 100 preparations CSE 0100 DNA preparation with centrifugation without enrichment GEN-IAL® QuickGEN* Sample preparation centrifugation DNA preparation of beverage samples, Centrifugation Beer – bacteria & yeast Qualitative multiplex real-time PCR GEN-IAL® First-Beer Differentiation PCR Kit Multiplex detection (30 species) and identification (19 species) of relevant beer spoilers 96 reactions TPBD 0096 GEN-IAL® First-Beer Differentiation PCR Kit for LC 480 Multiplex detection (30 species) and identification (19 species) of relevant beer spoilers for LC480 96 reactions TPBD 0096 LC480 GEN-IAL® QuickGEN* First-Beer Differentiation PCR Kit Multiplex detection (30 species) and identification (19 species) of relevant beer spoilers 96 reactions QTPBD 0096 GEN-IAL® QuickGEN* First-Beer Differentiation PCR Kit for LC 480 Multiplex detection (30 species) and identification (19 species) of relevant beer spoilers for LC480 96 reactions QTPBD 0096 LC480 GEN-IAL® QuickGEN* First-Beer Differentiation PCR Kit for MyGo Pro Multiplex detection (30 species) and identification (19 species) of relevant beer spoilers for MyGo Pro 96 reactions QTPBD 0096 MG GEN-IAL® First-Beer yeast and bacteria differentiation TaqMan™ Multiplex detection and identification of beverage spoiling bacteria and yeasts 96 reactions TPYB 0096 GEN-IAL® First-Beer yeast and bacteria differentiation TaqMan™ LC 480 Multiplex detection and identification of beverage spoiling bacteria and yeasts 96 reactions TPYB 0096 LC480 GEN-IAL® QuickGEN* First-Beer yeast and bacteria differentiation TaqMan™ MyGo Pro Multiplex detection and identification of beverage spoiling bacteria and yeasts for MyGo Pro 96 reactions QTPYB 0096 MG GEN-IAL® P1 Hyb Probe Screening LC 2.0 FRET DNA screening and differentiation of bacteria and yeasts for LC 2.0 50 reactions PP1H 0050 LC2.0 GEN-IAL® P1 Screening TaqMan™ DNA Screening and differentiation of beer spoiling bacteria and yeasts 50 reactions PP1T 0050 GEN-IAL® QuickGEN* P1 Screening TaqMan™ DNA screening and differentiation of beer spoiling bacteria and yeasts 50 reactions QPP1T 0050 GEN-IAL® QuickGEN* P1 Screening TaqMan™ DNA screening and differentiation of beer spoiling bacteria and hop resistance genes 50 reactions QPP1HR 0050 GEN-IAL® P1 Screening Spartan DX-12 TaqMan™ DNA screening without differentiation of bacteria and yeasts 50 reactions PP1T 0050 SP GEN-IAL® P1OHScreening Spartan DX-12 TaqMan™ DNA screening without differentiation of bacteria 50 reactions PP1TOH 0050 SP GEN-IAL® QuickGEN* First-Biofilm TaqMan™ Specific DNA detection of Lactococcus lactis, Leuconostoc mesenteroides and Pichia anomala 50 reactions QTPBF 0050 GEN-IAL® First Yeast Hyb Probe Screening LC 2.0 FRET DNA screening and differentiation of yeasts 50 reactions PYHYB 0050 LC2.0 * QuickGEN kits deliver a rapid, easy one-step DNA lysis and amplification method. Pre-enriched samples as well as centrifuged or filtrated samples may be used with CSE 0100 or FSE 0100 and subsequent QuickGEN detection kits. 72 Microbiology/hygiene Beverage analysis Product Description Beer – bacteria Qualitative real-time PCR GEN-IAL® Pectinatus spp./Megasphaera spp. TaqMan™ Specific DNA detection and differentiation of Pectinatus and Megasphaera Beer – resistance genes Qualitative real-time PCR GEN-IAL® hop resistance genes horA and horC / hitA and orf5 TaqMan™ No. of tests/amount Art. No. 50 reactions TPPMD Specific DNA detection of hop resistance genes 50 reactions TPHR 0050 GEN-IAL® QuickGEN* hop resistance genes horA and horC / hitA and orf5 TaqMan™ Specific DNA detection of hop resistance genes 50 reactions QTPHR 0050 Beer – yeast Qualitative real-time PCR GEN-IAL® Dekkera anomala TaqMan™ Specific DNA detection of Dekkera anomala 50 reactions TPYDA 0050 GEN-IAL® Pichia anomala TaqMan™ Specific DNA detection of Pichia anomala 50 reactions TPYPA 0050 GEN-IAL® Saccharomyces diastaticus TaqMan™ Specific DNA detection of Saccharomyces diastaticus 50 reactions TPYSD 0050 GEN-IAL® Pichia membranaefaciens TaqMan™ Specific DNA detection of Pichia membranaefaciens 50 reactions TPYPM 0050 GEN-IAL® Bottom fermented yeast TaqMan™ Specific DNA detection of bottom fermented yeast 50 reactions TPYUG 0050 GEN-IAL® Top fermented yeast TaqMan™ Specific DNA detection of top fermented yeast 50 reactions TPYOG 0050 GEN-IAL® accessories real-time PCR GEN-IAL® Dekkera bruxellensis Standards DNA standards for Dekkera bruxellensis quantification 200.000 cfu DBST 0100 Color Compensation Kit LC 480 Color compensation kit for multiplex assays 5 reactions PP1TCC 0005 Color Compensation Kit LC LightCycler Color compensation kit for multiplex assays 5 reactions CCFH 0005 Washing solution Washing solution for SEW 0100 43 ml WS 0100 100 determinations ZLP1002667 100 determinations 40 determinations R1091 R1092 Test systems for cleaning control AMP/ATP detection Bioluminescence LuciPac® Pen Test system for hygiene control on surfaces (based on detection of ATP/AMP) Reaction tubes with integrated swab for use with Lumitester PD-30 Protein tests Swab tests RIDA®CHECK Colorimetric test, ready-to-use swabs for the detection of protein residues on surfaces * QuickGEN kits deliver a rapid, easy one-step DNA lysis and amplification method. Pre-enriched samples as well as centrifuged or filtrated samples may be used with CSE 0100 or FSE 0100 and subsequent QuickGEN detection kits. 73 R-Biopharm AG – Product catalogue 2017 Equipment and accessories In laboratories equipment and machines are now routinely used to standardise analysis. Each analysis has specific needs and requirements which necessitates different accessories. This is where the team of technicians from R-Biopharm comes in. What is the right equipment required for each test? We develop matching applications for an even easier, faster and more efficient performance and analysis. Whether an automated processing of an ELISA by a fully automated analyser, or a portable analyser for lateral flow test. With the RIDA®SMART APP the quantitative evaluation of rapid tests is possible for the demand on-site. Our specialists improve and update these systems and devices continuously. That’s how we can offer you the best support with state of the art technologies for your laboratory or on-site testing. 74 The range of equipment and software covers the full portfolio and requirements of R-Biopharm products. The requirements for a high or low sample throughput in the laboratory will be observed: starting from optimal sample preparation, performance of test procedures, through analysing and evaluation for manually or fully automated applications for all products, their specific needs are considered. Everything for your analysis and performance just from one supplier. RIDA®SMART APP Test evaluation Smartphone application for the analysis of lateral flow tests ThunderBolt® Test running Automate for the processing of an ELISA RIDA®CUBE SCAN Single tests Small automate for enzymatic tests 75 R-Biopharm AG – Product catalogue 2017 Equipment/software/accessories Equipment Product Description ELISA Photometer ChroMate® 4300 Microtiter plate reader; suitable only for 450nm wavelenght with reference wavelenght at 630nm 1 ZCR4300 Microtiter plate photometer 1 ZELX800 BioTek® ELx800™ No. of tests/amount Art. No. Automates ChemWell®2910 1-microtiter plate analyser 1 ZCW2910 GEMINI 2-microtiter plate analyser 1 ZGEMINI ThunderBolt® 2-microtiter plate analyser 1 ZTB Bolt™ 1-microtiter plate analyser 1 ZBOLT Mycotoxin analysis HPLC automate RIDA®CREST Online handling system to be used in conjunction with IMMUNOPREP® ONLINE cartridges 1 ZRIDACREST 2000 RIDA®CREST ICE Automated cartridge exchange and high presure dispenser units to be used in conjunction with the IMMUNOPREP® ONLINE cartridges 1 ZRIDACREST 1500 Enzymatic analysis Autoanalyzer RIDA®CUBE SCAN 340/505 Analyser set Automatic analyser only for RIDA®CUBE test kits 1 set ZRCS0505 RIDA®CUBE SCAN 340/546 Analyser set Automatic analyser only for RIDA®CUBE test kits 1 set ZRCS0546 RIDA®CUBE SCAN 340/580 Analyser set Automatic analyser only for RIDA®CUBE test kits 1 set ZRCS0580 RIDA®CUBE SCAN Tablet PC Separate tablet for replacement 1 ZRCT0500 qPCR qPCR thermocycler 1 unit ZMX3005P1 Mx3005P real-time PCR instrument with notebook computer Mycotoxin rapid test RIDA®QUICK RIDA®SMART APP STAND NEXUS 6 Smartphone stand for NEXUS 6 1 ZRSAN6STAND RIDA®SMART APP STAND NEXUS 6P Smartphone stand for NEXUS 6P 1 ZRSAN6PSTAND 76 Equipment/software/accessories Equipment Product Description No. of tests/amount Art. No. CULTURA® Mini Incubator Incubator for incubations at 30 - 45 °C (Compact Dry, RIDA®STAMP, Pathogen ELISAs, microbiological MTP-format Vitamin analysis etc.) 1 ZC7140651 Lumitester PD-30 Luminometer for AMP/ATP measurement with LuciPac® Pen 1 ZLT-1402653 Lumitester PD-20/PD-30 Control Kit Positive control lamp with charger and negative control tubes for functional testing of Lumitester PD-20 and Lumitester PD-30 devices 1 ZLC1002657 R-Biopharm FP 50 Pipette 50 μl 1 unit Z0006 R-Biopharm FP 100 Pipette 100 μl 1 unit Z0007 R-Biopharm FP 1000 Pipette 1000 μl 1 unit Z0008 R-Biopharm FP 150 Pipette 150 μl 1 unit Z0009 Microbiology Pipettes Pipettes for RIDA®QUICK mycotoxin analysis PE-Pipettes 1 ml pipette for RIDA®QUICK tests 100 Z0005 PP-Test Tubes 50 ml test tubes for RIDA®QUICK tests 25 Z210261 RIDA®SOFT Win.NET Software for measurement, evaluation and documentation of RIDASCREEN® ELISAs 1 unit Z9996 Mycotoxines lateral flow tests RIDA®QUICK RIDA®SMART APP Software application for the NEXUS 6 and NEXUS 6P smartphone for the quantification of RIDA®QUICK RQS mycotoxin lateral flow tests. 1 voucher ZRSAM1000 Software ELISA 77 R-Biopharm AG – Product catalogue 2017 Equipment/software/accessories Accessories Product Description Mycotoxin analysis (HPLC) Immunoaffinity columns No. of tests/amount Art. No. PBS-Tablets Phosphate buffered saline tablets 100 (suitable for 10 l) RBRRP202 Immunoaffinity Column Rack Durable brass and PFTE rack allowing 6 samples to be processed at one time using Immunoaffinity columns 1 unit RBRCR1 Immunoaffinity Column Accessory Pack Glass barrels, syringes and adapters for use with all formats of RBR Immunoaffinity columns 10 each RBRAP01 Adapter Re-usable adapters for use with Immunoaffinity columns 10 units RBRRP200 Glass syringe barrels for use with Immunoaffinity columns 1 unit RBRRP201 Syringe pump unit Re-usable syringe and rubber connector for use with Immunoaffinity columns 1 each RBRRP203 Aflatoxin analysis KOBRA® CELL Electrochemical cell for derivatisation of aflatoxins B1 and G1 using HPLC 1 unit RBRK01 KOBRA® CELL Membrane Replacement membrane for the KOBRA® CELL 1 unit RBRK02 KOBRA® CELL Installation Pack Contains 5 metres of PEEK tubing, a tubing cutter, 10 ferrules and 3 unions 1 unit RBRK03 Stainless steel electrode Replacement stainless steel electrode for KOBRA® CELL 1 unit RBRK04 Platinum working electrode Replacement working electrode for KOBRA® CELL 1 unit RBRK05 Power Pack Replacement power pack for KOBRA® CELL 1 unit RBRK06 Spacer Replacement spacer 0.2 mm for KOBRA® CELL Replacement spacer 0.1 mm for KOBRA® CELL Replacement spacer 0.1 mm for KOBRA® CELL with reaction channel 1 unit RBRK07 RBRK08 RBRK09 Spacer grid Replacement spacer grid for KOBRA® CELL 1 unit RBRK10 Premi®Test Starter Kit Starterkit for Premi®Test, includes accessories 1 Set ZPT-2000 Premi®Test Multipress Sampling device to squeeze 12 sample at once 1 unit ZPT-2012 Real-time PCR SureFast® SureCycle For validation of thermocycler 4 tests F4001 SureCC Color Compensation Kit I Color Compensation for multiplex application of SureFood®/SureFast® kits on LC480 for 3 calibration runs F4009 SureCC Color Compensation Kit II Color Compensation for multiplex application of SureFood®/SureFast® kits on LC2.0 and 1.5 for 3 calibration runs F4010 SureInhibition Control Test for inhibiting substances 100 reactions F4050 SureFast® Animal+Plant Control Extraction control 100 reactions F4053 Premi®Test 78 Explanation International standardisation and regulation authorities AACCI American Association of Cereal Chemists International AFNOR Association Française de Normalisation AOAC Association of Official Analytical Chemists AOAC METHODS VALIDATION PROGRAMS: • AOAC- RI Performance Tested MethodsSM • AOAC-OMA Official MethodsSM • AOAC-PTM Peer-Verified MethodsSM CEN Comité Européen de Normalisation Codex Alimentarius Commission The Codex Alimentarius Commission, established by FAO and WHO in 1963 develops harmonised international food standards and “Codex Methods of Analysis”. The methods are primarily intended as international methods for the verification of provisions in Codex standards. Definition of Codex types of methods of analysis: (a) Defining Methods (Type I) e.g. R5 Mendez ELISA method (b) Reference Methods (Type II) (c) Alternative Approved Methods (Type III) (d) Tentative Method (Type IV) FGIS Federal Grain Inspection Service GIPSA Grain Inspection, Packers and Stockyards Administration IDF International Dairy Federation IFU International Federation of Fruit Juice Producers ISO International Organisation for Standardization MicroVal E uropean certification organisation for the validation and approval of alternative methods for the microbiological analysis of food and beverages OIV International Organization of Vine and Wine 79 R-Biopharm AG – Product catalogue 2017 The R-Biopharm Group – contact us R-Biopharm AG R-Biopharm Inc. R-Biopharm R-Biopharm España S.A.U. Rhône Ltd. R-Biopharm Latinoamérica R-Biopharm Brasil Ltda. R-Biopharm France R-Biopharm Italia Srl. R-Biopharm Neugen Pvt. Ltd. R-Biopharm China R-Biopharm Australia subsidiaries R-Biopharm AG An der neuen Bergstraße 17 64297 Darmstadt, Germany Phone: +49 (0) 61 51 - 81 02-0 Fax: +49 (0) 61 51 - 81 02-40 E-mail: sales@r-biopharm.de www.r-biopharm.com 80 Argentina R-Biopharm Latinoamérica S.A. Vuelta de Obligado 2943 C1429 AVC Ciudad de Buenos Aires Phone: +54 (0) 11 - 47 01 62 62 Fax: +54 (0) 11 - 47 01 62 62 E-mail: info@r-biopharmlat.com.ar India R-Biopharm Neugen Pvt. Ltd. 2nd & 3rd Floor, Plot No. 7, C.F. Area, Phase II, IDA Cherlapally, Hyderabad - 50 00 51 A.P., INDIA Phone: +91 (0) 40 - 2980 - 5251/-4251 /-2324/-2321 Fax: +91 (0) 40 - 27 26 60 - 33 E-mail: info@r-biopharm.in Australia R-Biopharm Australia 34 Woodfield Boulevard Caringbah, NSW 2229 Phone: +61 (2) 2 - 96 68 06 00 Fax: +61 (2) 2 - 96 68 85 33 E-mail: p.hill@labdiagnostics.com.au Italy R-Biopharm Italia Srl Via Morandi 10 20077 Melegnano MI Phone: +39 (0) 2 - 9 82 33 330 Fax: +39 (0) 2 - 9 83 41 00 E-mail: info@r-biopharm.it Austria R-Biopharm AG, Christoph Reiterich Phone: +43 (0) 66 41 - 35 21 22 Fax: +49 (0) 61 51 - 81 02 - 40 E-mail: c.reiterich@r-biopharm.de Netherlands R-Biopharm AG, Michael Fijnenberg Phone: +31 (0) 75 - 6 15 10 46 E-mail: m.fijnenberg@r-biopharm.nl Brazil R-Biopharm Brasil Rua José Bonifácio, 768 Jardim Flamboyant Campinas - SP CEP 13091-140 Phone: +55 (0) 19 33 05 73 51 Fax: +55 (0) 19 35 79 73 51 E-mail: vendas@r-biopharmbrasil.com.br China R-Biopharm Analysis Systems Trading (Beijing) Co. Ltd. Suite 526 Building C, ULO PARK, No. 603 Wangjingyuan, Chaoyang District Beijing, China 100102 Telefon: +86 (0) 10 - 84 58 32 18 Fax: +86 (0) 10 - 84 58 06 91 E-Mail: info@r-biopharm.cn France R-Biopharm France Parc d’affaires de Crécy 5a rue Claude Chappe 69370 Saint-Didier au Mont D’Or Phone: +33 (0) 4 78 - 64 32 00 Fax: +33 (0) 4 78 - 47 84 04 E-mail: standard@r-biopharm.fr Spain R-Biopharm España S.A.U. Sociedad Unipersonal Los Manzanos 4 28703 S.S. De Los Reyes Madrid Phone: +34 (0) 9 02 - 90 33 - 55 Fax: +34 (0) 9 16 - 54 89 92 E-mail: info@r-biopharm.es Switzerland R-Biopharm AG, Richard Blättler Phone: +41 (0) 26 - 67 00 15 - 7 Fax: +41 (0) 26 - 67 00 15 - 8 E-mail: r.blaettler@r-biopharm.ch United Kingdom R-Biopharm Rhône Ltd. Block 10 Todd Campus West of Scotland Science Park Acre Road, Glasgow Scotland, G20 0XA Phone: +44 (0) 14 - 19 45 29 - 24 Fax: +44 (0) 14 - 19 45 29 - 25 E-mail: info@r-biopharmrhone.com USA/Canada R-Biopharm Inc. 870 Vossbrink Dr. Washington, MO 63090, USA Phone: +1 (0) 8 77 - 7 89 - 30 33 Fax: +1 (0) 8 66 - 9 22 - 58 56 E-mail: info@r-biopharm.com 81 R-Biopharm AG – Product catalogue 2017 General terms & conditions of R-Biopharm AG (Date of issue: October 2010) I. General provisions These General terms & conditions only are valid for entrepreneurs, legal entities under public law or public-law special assets (legal entities according to § 310 I German Civil Code (“Bürgerliches Gesetzbuch” -”BGB”). We deliver according to these General terms & conditions exclusively. They are deemed to have been acknowledged with the placing of an order or the receipt of the goods and shall also apply to all future business relationships, even if they are not explicitly agreed upon again. Deviating terms and conditions are not binding for us, even if we do not object to them explicitly. II. Orders and offer documents Our offers are subject to alteration. Decisive for the scope of our delivery obligation are our offer in writing respectively our written order confirmation. Deliverable are only the products which are contained in our current applicable price lists. III. Prices and conditions of payment/Withdrawal in case of default 1. Purchase price is the price stated by us or - if no price has been stated - the price which is contained in our price list, which is in effect on the day of the order. The prices stated by us - unless otherwise stipulated in writing - are including packing and shipping costs, excluding VAT. The deduction of cash discounts shall not be granted. A small-quantity surcharge in the amount of 10 Euros can be charged for deliveries with a product value of up to 300 Euros (small quantity). 2. Payment obligations resulting from the delivery of goods are to be fulfilled within thirty (30) days of the invoice date by bank transfer exclusively and shall be deemed to have been effected only to the extent, to which we can dispose of them freely at a bank. For checks and bills of exchange, a processing fee of 30 Euros shall be charged; discounting and expenses shall be for the account of the Buyer. 3. The Buyer shall only be entitled to set-off with a counter-claim which is undisputed or has been determined by a final verdict. A right of retention the Buyer does only have as far as it is resulting from the same contractual relationship. 4. Should the Buyer be in default with due payments entirely or partly, the regulations of the statutory law are applicable. Interest in the amount of 8% above the basic interest rate (as it is published by the German Federal Bank) shall be due. We are reserving the right to claim any exceeding damage for delay. 5. In case of withdrawal, we are entitled - at the expense of the Buyer - to let the goods, which have been delivered by us, mark, store separately and collect. The Buyer already yet - is declaring his consent that the persons who are commissioned with the collection are entitled to access the premises, on which the goods are, and enter them by car for this purpose. 6. In case of our withdrawal, we are not obliged to further deliveries any more, also regarding further future deliveries. IV. Retention of title 1. We shall retain title to the goods delivered by us, until all the claims, to which we are entitled on whatever legal grounds arising from our business relationship with the Buyer, have been fully satisfied. Upon the Buyer’s request, we shall be obligated to release the securities in so far as their realizable value exceeds our claims by more than 10%. We reserve the right to select the items of collateral to be released. 2. The Buyer undertakes to only sell the goods, which are subject to retention, in his ordinary course of business, according to his usual terms and conditions of business and only as long as he is not in default with his payments. He is entitled to resell the goods, which are subject to retention, only on the condition that a transfer of the receivables, resulting from such a resale, to us takes place. He is not be entitled to dispose of the goods, which are subject to retention, in any another way (such as e. g. collateral assignment, pledging, leasing, lending, etc.). The Buyer is obligated to immediately notify us of any seizure or other interference by a third party, together with handing over of the documents which are necessary for an intervention. V. Delivery 1. Our delivery times are generally only approximate and not binding. 2. Uncontrollable incidents, for which we are not responsible, e. g. natural phenomena, war, orders of the authorities, embargo, unexpected delays in the delivery of essential components and other materials (“Force Majeure”), shall prolong the delivery time reasonably. This also applies, if these incidents occur during a delay in delivery or at a sub-supplier. However, the delivery time shall be prolonged by a maximum period of two (2) months. Should we also not be able to deliver after this time, then the Buyer as well as we themselves are entitled to withdraw from the contract. Any claims of damages of the Buyer for this reason are excluded. Should we withdraw from the contract, we shall immediately refund the Buyer any and all payments possibly rendered for not yet delivered goods. 3. Should the Buyer - despite reminder - not fulfill his payment obligations resulting from existing contracts, we shall only supply on advance payment from then on. 4. We are entitled to partial deliveries to a reasonable extent; here each partial delivery can be invoiced separately. In case of order on call, the call-off has to take place at least two (2) calendar weeks prior to the desired delivery date. VI. Shipment and passing of risk 1. Dispatch ex works or distribution warehouse shall be carried out at the expense of the Buyer. Shipping route and mode of dispatch shall be determined by us. We shall only be obligated to obtain a transport insurance, if explicitly instructed to do so by the Buyer in writing; the Buyer shall bear the costs for this insurance. 82 2. The passing of risk to the Buyer takes place as soon as the goods have been handed over to the haulage contractor respectively leave our factory or distribution warehouse for the purpose of dispatch; this also is valid, if we - by way of exception organize additional services, e. g. carriage prepaid shipping, delivery to the premises of the Buyer, or similar. In particular we are not liable for alteration or deterioration of the goods during transport or resulting from improper storage. Should we have notified the Buyer that the goods are ready for dispatch or collection, the risk passes on to the Buyer, if he does not have the goods delivered or collect them, despite of us having set him a reasonable period of time for doing so; regarding that, the passing of risk takes place at the beginning of the day which follows the day, on which the deadline has expired. VII. Warranty/liability 1. It is precondition for the execution of claims based on a defect, that the Buyer has performed his responsibilities to examine and complain according to § 377 of the German Commercial Code (“Handelsgesetzbuch” -”HGB”) correctly and completely. 2. We are liable for faultlessness of the goods corresponding to the state of the art. Features of samples and specimens as well as any statements regarding the condition of the goods, shall only be considered as an agreement on quality, if they explicitly have been agreed upon as determining the condition of the goods. Otherwise they are non-binding and do not free the Buyer from an own inspection of the goods concerning their suitability for his purposes. We neither grant guarantees with the content of a liability without fault nor any other kind of guarantees for quality and durability in the legal sense. 3. We are not liable for damages as far as they have been caused by improper storage of our products and/or their application contrary to the prescriptions - e. g. application after expiry of their shelf life or contrary to the direction for use - or as far as they have been caused by the Buyer in any other way. 4. The exceeding of use-by dates after the delivery does not entitle the Buyer to claims of any kind, but is deemed to be the usual condition. This is not the case, if the period between the date of delivery and the use-by date is less than four (4) calendar weeks. 5. We shall only be liable for damages, as far as we attributable have caused them by intent or gross negligence (disregard for the due care and attention to a very coarse extent); except in case of violation of essential contractual obligations (obligations, whose fulfillment enables the proper execution of the contract at all and on whose observance the contractual partner may rely regularly). In this last-mentioned case we are liable for each negligence with the restriction that - in case of violation of essential contractual duties by slight negligence - our liability is limited to the damage which typically is predictable. 6. Should we not have violated any essential contractual obligations in the sense mentioned before, we are not liable in cases of slight negligence. Unaffected by any limitation of liability contained in these General Terms & Conditions stay: Liability for intent, malice, initial inability, gross negligence, liability resulting from a guarantee (which, however, we generally not grant), bodily harms and other cases of legally compelling liability - in these cases the statutory law is valid (under exclusion of the Terms and Conditions of our contractual partner). 7. The regulations of this clause Warranty/Liability are valid for our contractual liability as well as liability resulting from tort (unaffected thereby stays the action for possession in case of tort, after statutory limitation has taken place, § 852 German Civil Code (“Bürgerliches Gesetzbuch” -BGB”)). 8. As far as our lability is excluded or limited, this shall also apply to the personal liability of our representatives, employees and vicarious agents and our liability for them. 9. As far as there is a defect of the goods, for which we are liable, the Buyer has to grant us the opportunity to execute subsequent performance within a term of generally two (2) calendar weeks, before the assertion of his further rights. In case that subsequent performance fails twice, in case of our refusal, or if subsequent performance is impossible, is delayed unreasonably or unreasonable for the Buyer due to other reasons, the Buyer may - according to his choice - execute his further legal rights, namely rescission or reduction of the purchase price and (regarding defects for which we are liable) claim of possibly occurred damages or compensation for possible futile expenditure, by which our liability is limited according to the preceding regulations. VIII. Burden of proof/export/effectiveness 1. With none of the stipulations of these General terms & conditions an alteration of the burden of proof is intended. 2. We are not liable for the correctness of information regarding foreign-trade which we provide to our best conscience; it is the Buyer´s responsibility to assess the compliance with foreign-trade regulations with regard to our products himself. 3. Should any of the regulations of our General Terms & Conditions be ineffective and/ or incomplete, the validity of the other regulations shall remain unaffected thereby. IX. Applicable law and place of jurisdiction 1. The contractual relationship shall be governed by the laws of the Federal Republic of Germany, which shall be applicable supplementary. The UN-convention on contracts regarding the International Sale of Goods (CISG) shall not apply. 2. Exclusive Place of Jurisdiction is Darmstadt (Germany). However, we are entitled to file a lawsuit against the Buyer also at any other court, which does have jurisdiction regarding him according to the general regulations. 83 R-Biopharm AG R-Biopharm AG 03/2017 An der neuen Bergstraße 17 64297 Darmstadt, Germany Phone: +49 (0) 61 51 - 81 02-0 Fax: +49 (0) 61 51 - 81 02-40 E-mail: info@r-biopharm.de www.r-biopharm.com R-Biopharm – for the safety of your analysis.
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