TriLink CleanCap mRNAキャンペーン
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TriLink BioTechnologies
CleanCap® mRNA
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mRNAmRNA/ TriLinkCleanCap® mRNAin vivo mRNA mCAPARCA CleanCap® mRNA
· (94%+)mRNA · · Cap0(ARCA)in vivo · · mRNA
/ mRNA (Cas9, Cas9 Nickase, Cre) (EGFP, mCherry, FLuc, Renilla Luc, -gal) (EPO) mRNA (Cyanine5EGFP, Cyanine5Luciferase) (OVA)
CleanCap® Reagent AG CleanCap® Reagent AG (3-OMe)
RNA RNATriLink()
CleanCap®
CleanCap® 90-99%
ARCA 60-80%
CleanCap® FLuc 99% 5Capped
Enzymatic 95-99%
Cap1
Uncapped
ARCA FLuc 69% 5Capped
Cap0
Uncapped
ARCA EGFP mRNA
CleanCap® EGFP mRNA
CHO Cells 6 minute fusion Fuse-It-mRNA Kit 1g mRNA 72 h post fusion
Foreskin fibroblast cells, 10 minute fusion Fuse-It-mRNA Kit 1g mRNA 24 h post fusion
iPS derived cardiomyoctyes, 10 minute fusion Fuse-It-mRNA Kit 1g mRNA 24 h post fusion
ARCA EGFP mRNA
Data courtesy of Beniag CleanCap® EGFP mRNA
mRNA
mRNAUridineUridinePseudo Uridine5-methoxy Uridine(5moU) (Fluc) mRNA(= ) TriLinkCleanCap®5moUmRNA
Jurkat: 500ng mRNA + 1µL jetMESSENGER
MCF 10A: 500ng mRNA + 1µL jetMESSENGER
Data courtesy of Polyplus-transfection
Thp-1 Dual Cells (InvivoGen)
No.
Cas9 mRNA
() ()
556-33821 L-7606-20
552-33823 L-7606-100
550-33824 L-7606-1000
-
L-7606-5
554-33741 L-7206-20
550-33743 L-7206-100
558-33744 L-7206-1000
-
L-7206-5
551-33751 L-7207-20
557-33753 L-7207-100
555-33754 L-7207-1000
-
L-7207-5
Cre mRNA
CleanCap® Cas9 mRNA
-
CleanCap® Cas9 mRNA (mod)
5-methoxyuridine
CleanCap® Cas9 Nickase mRNA (5moU)
5-methoxyuridine
20g 100g
1mg 1mg×5
20g 100g
1mg 1mg×5
20g 100g
1mg 1mg×5
31,000 74,000 396,000
37,000 89,000 472,000
37,000 89,000 472,000
21,700 51,800 277,200
25,900 62,300 330,400
25,900 62,300 330,400
559-33791 L-7211-100
555-33793 L-7211-1000
-
L-7211-5
EGFP mRNA
CleanCap® Cre mRNA (5moU)
5-methoxyuridine
100g 1mg
1mg×5
74,000 396,000
51,800 277,200
552-33801 L-7601-100
558-33803 L-7601-1000
-
L-7601-5
556-33701 L-7201-100
552-33703 L-7201-1000
-
L-7201-5
550-33841 L-7701-100
556-33843 L-7701-1000
-
L-7701-5
mCherry mRNA
CleanCap® EGFP mRNA
-
CleanCap® EGFP mRNA (5moU)
5-methoxyuridine
CleanCap® Cyanine 5 EGFP mRNA (5moU)
Cyanine 5 5-methoxyuridine
-
100g 1mg
1mg×5 100g
1mg 1mg×5 100g
1mg 1mg×5
74,000 396,000
89,000 472,000
150,000 787,680
51,800 277,200
62,300 330,400
105,000 551,376
550-33721 L-7203-100
556-33723 L-7203-1000
-
L-7203-5
Luciferase mRNA
CleanCap® mCherry mRNA (5moU)
5-methoxyuridine
100g 1mg
1mg×5
74,000 396,000
51,800 277,200
559-33811 L-7602-100
555-33813 L-7602-1000
-
L-7602-5
553-33711 L-7202-100
559-33713 L-7202-1000
-
L-7202-5
557-33851 L-7702-100
553-33853 L-7702-1000
-
L-7702-5
557-33731 L-7204-100
553-33733 L-7204-1000
-
L-7204-5
-gal mRNA
CleanCap® FLuc mRNA
-
CleanCap® FLuc mRNA (5moU)
5-methoxyuridine
CleanCap® Cyanine 5 FLuc mRNA (5moU)
Cyanine 5 5-methoxyuridine
CleanCap® Renilla Luc mRNA (5moU)
5-methoxyuridine
100g 1mg
1mg×5 100g
1mg 1mg×5 100g
1mg 1mg×5 100g
1mg 1mg×5
60,000 317,000
74,000 396,000
132,000 699,360
74,000 396,000
42,000 221,900
51,800 277,200
92,400 489,552
51,800 277,200
553-33831 L-7608-100
559-33833 L-7608-1000
-
L-7608-5
558-33761 L-7208-100
554-33763 L-7208-1000
-
L-7208-5
CleanCap® beta gal mRNA CleanCap® beta gal mRNA (5moU)
5-methoxyuridine
100g 1mg
1mg×5 100g
1mg 1mg×5
74,000 396,000
89,000 472,000
51,800 277,200
62,300 330,400
EPO mRNA
555-33771 L-7209-20
551-33773 L-7209-100
559-33774 L-7209-1000
-
L-7209-5
CleanCap® EPO mRNA (5moU)
5-methoxyuridine
20g 100g
1mg 1mg×5
31,000 74,000 396,000
21,700 51,800 277,200
OVA mRNA
554-35505 L-7610-20
552-35501 L-7610-100
558-35503 L-7610-1000
-
L-7610-5
552-33781 L-7210-100
558-33783 L-7210-1000
-
L-7210-5
CleanCap® OVA mRNA CleanCap® OVA mRNA (5moU)
5-methoxyuridine
20g 100g
1mg 1mg×5 100g
1mg 1mg×5
25,000 60,000 317,000
74,000 396,000
17,500 42,000 221,900
51,800 277,200
Cas9 mRNABroadMITHarvardIowaUTokyoRockefeller
CleanCap®
CleanCap® Reagent AGCleanCap® Reagent AG(3-OMe)mRNAin vitroTriLink CleanCap® AG5-N7-CleanCap® AG(3OMe)ARCAmRNA5-N7--3-O-
No.
CleanCap® Reagent
559-35511
N-7113-1
553-35514
N-7113-5
555-35513
N-7113-10
CleanCap® Reagent (3'-OMe)
553-35531
N-7413-1
557-35534
N-7413-5
559-35533
N-7413-10
CleanCap® Reagent AG CleanCap® Reagent AG (3'-OMe)
()
1mol 5mol 10mol
35,000 161,000 294,000
1mol 5mol 10mol
43,000 189,000 370,000
RNA
RNATriLinkRNARNA RNAmRNA
: µg~mg : Phosphorothioate2-OMePseudo-U5-Methyl-C : CleanCap®ARCAmCAP : HPLCPAGE ()
RNA CleanCap®mRNA
RNA
TriLinkDNA !
ORF TriLinkDNA DNA ()
TriLink BioTechnologies TriLink BioTechnologies RNA
20X04.501J
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