Product Components List

SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian

Product Details

Catalog Nos.Amount
63448524 rxns
63448648 rxns
63448796 rxns
634488192 rxns

Description

The SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing from 10–1000 cells or 250 pg–10 ng inputs of purified total RNA. This kit incorporates Takara Bio's proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. The kit was specifically designed to deliver highly sensitive and reproducible data from low-input RNA while keeping the workflow short and user friendly. The incorporated UMIs (Unique Molecule Identifiers) help customers perform UMI-based correction to obtain more reliable results. The kit does not require additional rRNA removal methods and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA.

Package Contents

For storage conditions, please see the Certificate of Analysis supplied with each component.

Product Documents and Information

Documents for our products are available for download at takarabio.com/manuals.

Notice to Purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Your use of this product is also subject to compliance with any applicable licensing requirements described on the product's web page at takarabio.com. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements.

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