Product Components List
SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
Product Details
Catalog Nos. | Amount |
---|---|
634485 | 24 rxns |
634486 | 48 rxns |
634487 | 96 rxns |
634488 | 192 rxns |
Description
The SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing from 10–1000 cells or 250 pg–10 ng inputs of purified total RNA. This kit incorporates Takara Bio's proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. The kit was specifically designed to deliver highly sensitive and reproducible data from low-input RNA while keeping the workflow short and user friendly. The incorporated UMIs (Unique Molecule Identifiers) help customers perform UMI-based correction to obtain more reliable results. The kit does not require additional rRNA removal methods and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA.
Package Contents
- 634485 (24 rxns):
- SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Components (Cat. No. 634489; Not sold separately) ▶️ View Components
- SMARTer RNA Unique Dual Index Kit - 24U (Cat. No. 634451) ▶️ View Components
- 634486 (48 rxns):
- SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Components (Cat. No. 634490; Not sold separately) ▶️ View Components
- SMARTer RNA Unique Dual Index Kit - 24U (Cat. No. 634451) ▶️ View Components
- 634487 (96 rxns):
- SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Components (Cat. No. 634491; Not sold separately) ▶️ View Components
- SMARTer RNA Unique Dual Index Kit - 24U (Cat. No. 634451) ▶️ View Components
- 634488 (192 rxns):
- SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Components (Cat. No. 634492; Not sold separately) ▶️ View Components
- 2 x SMARTer RNA Unique Dual Index Kit - 24U (Cat. No. 634451) ▶️ View Components
For storage conditions, please see the Certificate of Analysis supplied with each component.
Product Documents and Information
Documents for our products are available for download at takarabio.com/manuals.
- SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian User Manual
Notice to Purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Your use of this product is also subject to compliance with any applicable licensing requirements described on the product's web page at takarabio.com. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements.
© 2020 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
This document has been reviewed and approved by the Quality Department.