cytivaco VIA Freeze™ controlled-rate freezers provide a scalable and flexible solution to your cryopreservation needs. Available in a range of capacities, they combine customizable cooling profiles,
VIA Freeze liquid nitrogen-free controlledrate freezers CELL THERAPY SYSTEMS VIA FreezeTM controlled-rate freezers provide a scalable and flexible solution to your cryopreservation needs. Available in a range of capacities, they combine customizable cooling profiles, electronic control systems, and a conduction cooling method to optimize cGMP-compliant cryopreservation without the need for liquid nitrogen. All VIA Freeze models integrate with ChronicleTM automation software* enabling cooling profiles to be developed and shared across networked units. Controlled cooling · Maintain optimal cell function and viability with therapyspecific cooling profiles and precise temperature control · Develop, store, and share profiles using the VIA Freeze user interface or within Chronicle automation software Consistent quality · Remove heat evenly from all samples with liquid nitrogen-free conduction cooling · Standardize cryopreservation by delivering stored profiles between networked units Cleanroom compatible · Fill and freeze containers in the same location to streamline manufacturing processes · Eliminate risks and infrastructure costs associated with liquid nitrogen cGMP-compliant cryopreservation · Ready to integrate with cGMP process validation procedures · Controls designed to support 21 CFR Part 11 compliance Versatile · Increase productivity without changing processes between VIA Freeze models · Options from 24 to 384 cryogenic vials, cryobags filled up to 270 mL, and straws. Customized plates also available Fig 1. VIA Freeze Duo liquid nitrogen-free controlled rate freezer, part of a range to suit all processing requirements. * Can be purchased separately cytiva.com Configuration Performance and structural aspects of VIA Freeze controlled-rate freezers are identical (see Table 2). It is only the capacity of the different models that varies. Simply match your production volume with the capacity of the VIA Freeze model to ensure consistent sample quality and biological outcomes. Suitable for vials, bags, and straws Our sample plates and adapters provide the flexibility and scalability your cryopreservation process needs. Available in different formats to suit your preferred container type, each plate has a different container capacity and can be fitted on one or across multiple heat transfer pads (see Table 1 for a selection of available plates). To load samples, simply select the adapter for your specific container type and place it in your VIA Freeze cryochamber. It is also possible to quickly change to a different plate if you need to freeze samples in other containers. (1) Fig 2. Capacity varies but controlled cooling performance remains consistent across the range: VIA Freeze, VIA Freeze Duo, VIA Freeze Quad (left to right). cGMP support Each unit includes a calibration certificate. All models support webbased process monitoring. IQOQ documentation is available for each model. 4 (2) 1 2 3 5 1 Cryochamber 2 Heat transfer pad 3 Sample plate release clips (removed when fitting large plates) 4 Standard sample plate fits to one heat transfer pad (example shows 48 × 2 mL cryovial plate) 5 Double width sample plate spans two heat transfer pads (example shows 4 × 250 mL cryobag plate with metal cassettes) Fig 3. The Stirling cryocooler engine powered VIA Freeze controlled-rate freezers give you complete control over freezing samples in a range of container types (cut-away image of VIA Freeze Quad unit shown). Fig 4. VIA Freeze Duo cryochamber loaded with two sample plates fitted on separate heat transfer pads with (1) two single 50 mL horizontal cryobags plates and (2) two single 2 mL 48 cryovial plates. 2 Table 1. Capacities of commonly used sample plates compatible with VIA Freeze units. More options are available; full information on the range of sample plates is available on the Cytiva website. VIA Freeze VIA Freeze Duo VIA Freeze Quad Cryovial type and capacity by VIA Freeze unit* Cryovial manufacturer Type Corning, NalgeneTM, 2 mL NuncTM, FluidX, Greiner, CellSealTM/Cook Regentec West Pharma 2 mL West Pharma 5 mL Aseptic Technologies 50 mL Aseptic Technologies 6, 10 mL Aseptic Technologies 2 mL CellSeal/Cook Regentec 5 mL Cryobag type and capacity by VIA Freeze unit Cryovials per plate 48 35 20 6 12 15 10 Total cryovials per unit 48 96 192 35 70 140 20 40 80 6 12 24 12 24 48 15 30 60 10 20 40 Cryobag manufacturer BioSafeTM, Aviamax, BioArchiveTM Origen, CryoMACSTM Origen, CryoMACS Origen, CryoMACS Origen, CryoMACS Origen, CryoMACS Type 50 mL Max. fill volume 25 mL Orientation Vertical 50 mL 50 mL 250 mL 500 mL > 1000 mL 20 mL 20 mL 70 mL 100 mL 270 mL Horizontal Vertical Vertical Vertical Horizontal NA Not compatible with this VIA Freeze model +/- x8 2 mL vials Sample plate spans two sample plate loading trays Sample plate spans four sample plate loading trays * Maximum cryovial fill volume may not be feasible when the plate is fully loaded with cryovials Cryobags per sample plate 4 Total cryobags per unit 4 8 16 1 1 2 4 4 4 8 16 4 NA 4 8 2 NA NA 4 1 NA NA 1 Note: Bespoke sample plates can be made for container types to order. Some sample plates require additional accessories. Information on adapter plates for microwells, plates, and straws is available on request. Optimize and monitor cryogenic steps with Chronicle automation software* Once you have developed your cooling profiles, standardize and integrate your processes with a digital solution designed to optimize complex cell therapy process development and manufacturing. Chronicle automation software can monitor all facility manufacturing operations and supply chain logistics with real-time data acquisition and notifications. By integrating your VIA Freeze units, you will be able to define optimized, therapy-specific controlled cooling profiles and deliver them to all networked units. You will also be able to constantly monitor linked instruments, create and annotate electronic batch records of your run data, plus access records and instrument alerts from the VIA Freeze touchscreen or any web browser. Fig 5. Chronicle automation software connects Cytiva cell therapy instruments and third-party instruments and supports process development through commercial GMP manufacturing. Chronicle automation software: · Unites cooling run data with complete cell processing electronic batch records · Provides rapid adoption of cryopreservation processes during scale up · Offers remote monitoring of processes and equipment * Can be purchased separately 3 Table 2. VIA Freeze specifications. Liquid nitrogen-free Cleanroom optimized Minimum temperature Maximum number of standard samples plates User Access Control PDF batch report User-programmable cooling protocols Pre-programmed cooling protocols Dimensions (W×D×H) Weight Power VIA Freeze Yes Yes -100ºC 1 Included Yes 30 Yes 29.3 × 35.9 × 44.9 cm 14 kg (31 lb) 120240 VAC 200 W (max) Ordering information Product VIA Freeze controlled-rate freezer VIA Freeze Duo controlled-rate freezer VIA Freeze Quad controlled-rate freezer Product code VF_30001 VFD_30006 VFQ_30010 VIA Freeze Duo Yes Yes -100ºC 2 Included Yes 30 Yes 44.6 × 38.3 × 61 cm 40 kg (88 lb) 120240 VAC 350 W (max) VIA Freeze Quad Yes Yes -100ºC 4 Included Yes 30 Yes 63.8 × 38.3 × 61 cm 64 kg (141 lb) 120240 VAC 650 W (max) cytiva.com/cryo For local office contact information, visit cytiva.com/contact Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC or an affiliate. Chronicle, and VIA Freeze are trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva. Biosafe is a trademark of Biosafe S.A. BioArchive is a trademark of ThermoGenesis Corp. CellSeal is a trademark of Cook General Biotechnology, LLC. CryoMACS is a trademark of Miltenyi Biotec GmbH. Nalgene and Nunc are trademarks of Thermo Fisher Scientific. All other third party trademarks are the property of their respective owners. © 2019-2020 Cytiva All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information. CY11772-27Feb20-DFAdobe PDF Library 15.0 Adobe InDesign 14.0 (Macintosh)