truChIP Chromatin Sample Preparation Solutions

500230 CP01 cryoPREP Manual Pulverizer Tissue disruption tool to process fresh frozen tissue with masses in the range of 15 mg to 1 g. For the CP01, only TT05 and TT01 tissue bags are required. 500497 CP02 Low and High…

truChIP Chromatin Sample Preparation Solutions - Covaris

CP01 cryoPREP Manual Pulverizer. Tissue disruption tool to process fresh frozen tissue with masses in the range of 15 mg to 1 g. For the CP01, only TT05 and ...

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Covaris

www.covaris.com

truChIP� Chromatin Sample Preparation Solutions
Genome-wide Profiling Done with Confidence
ChIP-Seq is a powerful sequencing technology that is used to study genome-wide protein-DNA interactions in vivo. The sample preparation procedure for ChIP-Seq is challenging due to a myriad of technical factors such as cell inputs, antibody quality, and the abundance of the targeted epitope. Covaris offers a simplified and reliable sample preparation workflows enabling investigators to confidently IP protein-bound DNA for sequencing.

ChIP Publication Counts for Covaris

"The sample preparation workflow for ChIP-Seq is very challenging. After using other methods that failed to work well, our laboratory decided to evaluate the Covaris workflow. We value the system for its efficient handling of small sample volumes and its high reproducibility."
- Rui Yi, PhD. Principal Investigator at The University of Colorado, Boulder
Covaris AFA�

Water Bath Sonicator

Publish with Confidence Covaris delivers the most consistent sample preparation workflow available allowing investigators to publish the highest quality data. A side-by-side comparison of 20 million raw 264.7 monocytes using AFA and traditional water bath sonication was performed.

truChIP Advantages for Cell Lines Protein-DNA interactions maintained for IP Optimized for use with all mammalian cells Faster protocol and lower sample failure rates

truChIP Advantages for Tissues Compatible with soft and hard tissue types
Simplified tissue ChIP workflow Non-contact dry tissue pulverization

Covaris

www.covaris.com

Product Information

Part #

Product Name

520156

truChIP Ultra-Low Chromatin Shearing Kit with Formaldehyde

520154

truChIP Chromatin Shearing Kit with Formaldehyde

520237

truChIP Chromatin Shearing Tissue Kit with Formaldehyde

Description
Includes reagents required to run ultra-low cell (<100k) protocol. AFA processing tubes are sold separately.
Includes reagents required to run the chromatin shearing protocol to process up to 3M cells in the microTUBE and 30M cells in the milliTUBE. AFA processing tubes are sold separately.
Includes reagents required to process both low tissue mass inputs (20 to 50 mg in the microTUBE) and high tissue mass inputs (up to 120 mg in the milliTUBE). AFA processing tubes are sold separately.

500001

CP02 cryoPREP Pulverizer

Automatic tissue disruption tool to process fresh frozen tissue with masses in the range of 15 to 100 mg.

500230 500497

CP01 cryoPREP Manual Pulverizer

Tissue disruption tool to process fresh frozen tissue with masses in the range of 15 mg to 1 g. For the CP01, only TT05 and TT01 tissue bags are required.

CP02 Low and High Tissue Mass Starter Kit

Includes parts and accessories to process low (<50 mg to 1 g) and high (1 to 2 g) tissue samples for downstream applications including tissue ChIP.

Workflow

Fix Tissue

Flash Freeze

Pulverize Tissue
(cryoPREP)

Fix Cells

AFA Advantage

Isolate Nuclei

Shear Chromatin

Reverse Crosslink

Purify DNA

Library Preparation

Sequencing

Information subject to change without notice. For research only. Not for use in diagnostic procedures.

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