User Manual www.usascientific.com PO Box 3565 Ocala, FL 34478 Phone: 800-522-8477 352-237-6288 Fax: 352-351-2057 info@usascientific.com www.usascientific.com USA Scientific, Inc. Tolerance Check Repeating Pipette
Repeating Pipette User Manual ® www.usascientific.com User Manual 1. General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1.1. Design Principle of the Repeating Pipette . . . . . . . . . . . . . 4 2. Volume Selection and Choice of Tips . . . . . . . . . . . . . . . . . 5 2.1. TipOne® Repeating Pipette Tips . . . . . . . . . . . . . . . . . . . . . 6 2.2. Using the 25 and 50 ml Tip Adapters . . . . . . . . . . . . . . . . . 6 3. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 3.1. Inserting the Tip . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . 7 3.2. Filling the Tip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 3.3. Dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3.4. Removing the Tip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 4. Safety Precautions and Operational Limitations . . . . . . . . . 9 5. Troubleshooting Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 6. Maintenance, Service and Warranty . . . . . . . . . . . . . . . . . 11 7. Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 8. Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 9. Quick Reference Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 10. Manufacturing and Inspection Standards . . . . . . . . . . . . 15 3 1. General The repeating pipette is a hand-held device for repetitive dispensing. Up to 48 aliquots of the same sample can be made from a single filling, resulting in a considerable reduction in time and expense. The repeating pipette allows the selection of five separate volumes from each TipOne repeating tip. Eight different tip sizes provide the most important volumes from 2 to 5000 µl. Use only original USA Scientific TipOne repeating tips to guarantee performance. Fig.1 4 1.1. Design Principle of the Repeating Pipette (Fig.1) Volume selection dial To determine the dispensing volume, the dial setting (1 5) is multiplied by the minimum aliquot volume of the TipOne repeating tips. Dispensing lever To dispense, press the dispensing lever down until it stops. Filling lever To fill the tip, slide the filling lever upward. Locking clamp To secure the tip, tighten the locking clamp down over the top of the tip. TipOne repeating pipette tip 2. Volume Selection and Choice of Tips For repetitive dispensing of a specific volume: Find the desired volume in the columns on the right. The required dial setting and maximum number of pipetting steps for the volume are shown at the top of the columns. Select the corresponding TipOne repeating tip from the column on the left. TIP SELECTION GUIDE Dispensing Volumes of TipOne Repeating Pipette Tips with the 4736-8025 Repeating Pipette Selection Dial Setting 1 2 3 4 Maximum Pipetting Steps 48 23 15 11 0.1 ml tip 2 µl 4 µl 6 µl 8 µl 0.5 ml tip 10 µl 20 µl 30 µl 40 µl 1.0 ml tip 20 µl 40 µl 60 µl 80 µl 2.5 ml tip 50 µl 100 µl 150 µl 200 µl 5.0 ml tip 100 µl 200 µl 300 µl 400 µl 10.0 ml tip 200 µl 400 µl 600 µl 800 µl 25.0 ml tip 500 µl 1.0 ml 1.5 ml 2.0 ml 50.0 ml tip 1.0 ml 2.0 ml 3.0 ml 4.0 ml 5 5 8 10 µl 50 µl 100 µl 250 µl 500 µl 1000 µl 2.5 ml 5.0 ml *Adapter necessary for 25 ml and 50 ml tips 2.1. TipOne® Repeating Pipette Tips The minimum dispensing volume and the maximum filling capacity is printed on each tip (Fig. 2). The scale facilitates drawing off partial quantities and helps you to estimate the amount of liquid still available for operation. The volume to be dispensed is obtained by multiplying the number selected on the volume selection dial by the minimum dispensing volume of the tip. For example, the 1.0 ml tip has a minimum volume where 1=20 l. At setting 4, the dispensed volume will be 4 x 20 l or 80 l. For increased precision, choose a small capacity tip and use a high dial setting. The correlation between the position of the volume selection dial and the number of dispensing steps is as shown in the table. Fig. 2 Number of Pipetting Steps Position of Volume Selection Dial 48 1 23 2 15 3 2.2. Using the 25 and 50 ml Tip Adapters (Fig.3) Attach the adapter to the 25 ml or 50 ml tips by turning it clockwise; remove by turning it counter clockwise. 11 4 8 5 6 Fig. 3 3. Operation 3.1. Inserting the Tip Slide the filling lever down until it stops. Raise the locking clamp upward. Insert the tip until it clicks into position. The tip plunger must be completely inside the tip barrel before attaching it to the repeating pipette (Fig. 4). Fig. 4 With the filling lever in the down position, lower the locking clamp until it clicks to secure the tip in place (see Fig. 5). 3.2. Filling the Tip narrow-necked vessels or piercing of sealing foil and also makes dispensing of small volumes more precise. Immerse the tip into the liquid. Slowly slide the filling Use the following steps for lever upward (Fig. 6). all sizes of tips. 0.1 ml, 0.5 ml, 1.0 ml, and 2.5 ml Discard the liquid from tips may also be used with the first dispensing a standard 200 µl pipette step. The repeating tip. Attaching a tip pipette is now ready for facilitates filling from operation. Fig. 5 Fig. 6 7 Additional Information Raising the filling lever too quickly can cause an excessive vacuum. This may cause tiny air bubbles to accumulate in the liquid, resulting in inaccurate volumes. Important: The first dispensing step must be discarded so that any residual filling pressure may be released and the system is prepared for precise delivery. Small bubbles in the tip beneath the piston will not affect the accuracy since the residual stroke lock prevents the delivery of any remaining sample after the last dispensing step. It is not necessary to completely fill the tip. Partial filling will not cause dispensing inaccuracies. 3.3. Dispensing (Fig.7) Check the volume selection dial. Wipe the end of the tip with lint-free tissue. Hold the tip so it touches the inner wall of the vessel. Dispense the liquid by completely depressing the dispensing lever. Fig. 7 8 Additional Information It is not necessary to attach a standard 200 µl pipette tip to the 5.0 ml or 10.0 ml repeating tips. Never attach a standard pipette tip when working with the 25 ml or 50 ml repeating tips. A new tip must be used each time to avoid carry-over and contamination. 3.4. Removing the Tip (Fig.8) Hold the repeating pipette over a beaker. Slowly depress the filling lever down to the stop. This will completely empty the tip. Raise the locking clamp upward. Remove the tip. Note: 25 ml or 50 ml tips are removed from the reusable adapter by turning counter clockwise. Fig. 8 4. Safety Precautions and Operational Limitations When removing the repeating pipette tips, please note the following: Do not touch the tip, especially when using hazardous reagents! Empty the tips into a proper disposal bin to avoid splashing and aerosols. Treat any retained fluid as a bio-hazard. Do not allow the fluid to come into contact with laboratory personnel. If a liquid with a surface tension significantly different from water is being dispensed, inaccuracies or leaking can occur. The repeating pipette with an attached, partially filled tip should be stored in a stand to prevent air from entering the pipette. 9 5. Troubleshooting Guide Error Tip cannot be inserted. Unable to fully raise the filling lever. Excessive air bubbles develop during filling. Inconsistent dispensing. Tip drips. The repeating pipette jams or does not function. Cause The locking clamp cannot be fully raised unless the filling lever is completely down. The tip piston is not completely inserted in the barrel. Locking clamp incompletely depressed. Filling lever slightly lifted before immersing into the liquid. The tip is leaking. Volume selection dial is not set into position. The tip is leaking. The tip is not tightly attached. 10 Solution Depress the filling lever downward and raise the locking clamp as far as it will go. Slide the piston into the proper position. Completely depress the locking clamp. Draw a small amount of solution into the tip and discard it completely. Refill the tip. Replace the tip with a new one. Turn the volume selection dial into position until it clicks. Replace the tip with a new tip. Use more force to attach the tip. Call 800-522-8477 or 352-237-6288 for assistance. 6. Maintenance, Service and Warranty Maintenance External surfaces of the pipette can be cleaned with warm water or isopropyl alcohol. There are no other maintenance requirements. Important: Under no circumstances should the pipette be dismantled! Do not autoclave the pipette or the tips! Service If a problem cannot be solved with the aid of the Troubleshooting Guide, call USA Scientific at 800-522-8477 or 352-237-6288 for repair or replacement. Important: Decontamination of the pipette will be required before a return. We will send you a decontamination form to complete. Please contact USA Scientific for more detailed information. 11 Warranty Please check that the unit has been received complete and undamaged. Save all packaging and documents until you have thoroughly inspected your shipment; if you find that your order is incorrect or damaged please call USA Scientific. This warranty is valid for 24 months with the condition that the product has been used and cared for according to this User Manual. No liability is accepted for loss or damage arising from incorrect use. The liability is limited to the repair or replacement of the unit. USA Scientific is not liable for any consequential damages. We refer to our general terms of sale. USA Scientific reserves the right to alter these specifications without prior notice. 7. Ordering Information Catalog No. TipOne Repeating Pipette and Adapters Description Quantity 4736-8025 Repeating pipette 1 4756-2500 Adapter for 25 ml TipOne tips 1 4756-5000 Adapter for 50 ml TipOne tips 1 * Please call or visit our website for information on sterile adapters. Important Note: Please use only the accessories recommended by USA Scientific. Using non-recommended consumables can reduce the precision, accuracy, and life of the pipette. We do not honor any warranty or accept any responsibility for damage resulting from such action. Catalog No. 4751-0010 4751-0050 4751-0100 4751-0250 4751-0500 4751-1000 4751-2500 4751-5000 TipOne® Repeating Pipette Tips Volume 0.1 ml 0.5 ml 1.0 ml 2.5 ml 5.0 ml 10.0 ml 25.0 ml 50.0 ml Quantity 100 100 100 100 100 100 100 100 12 TipOne® Repeating Pipette Tips, Sterile Catalog No. Volume 4761-0010 4761-0050 4761-0100 4761-0250 4761-0500 4761-1000 4761-2500 4761-5000 0.1 ml 0.5 ml 1.0 ml 2.5 ml 5.0 ml 10.0 ml 25.0 ml 50.0 ml Quantity 100 100 100 100 100 100 100 100 8. Technical Data Tip Size 0.1 ml 0.5 ml 1.0 ml 2.5 ml 5.0 ml 10.0 ml 25 ml 50 ml Dispensing Volume Minimum Maximum 2 µl 10 µl 10 µl 50 µl 20 µl 100 µl 50 µl 250 µl 100 µl 500 µl 200 µl 1000 µl 500 µl 2500 µl 1000 µl 5000 µl Systematic Error (Inaccuracy) Minimum Maximum ± 1.6 % ± 1.6 % ± 1.0 % ± 1.0 % ± 0.9 % ± 0.9 % ± 0.8 % ± 0.8 % ± 0.6 % ± 0.6 % ± 0.5 % ± 0.5 % ± 0.4 % ± 0.4 % ± 0.3 % ± 0.3 % Random Error (Imprecision; CV) Minimum Maximum 5.0 % 2.5 % 1.5 % 0.8 % 0.9 % 0.6 % 0.8 % 0.5 % 0.6 % 0.3 % 0.6 % 0.3 % 0.6 % 0.25 % 0.5 % 0.25 % 13 Test conditions in accordance with EN ISO 8655 Liquid: Test Temperature: Water bi-distilled, degassed 2025°C, ±0.5 % constant Operating Temperature:+4 °C +40 °C Measurement: Performed with USA Scientific repeating pipette tips in accordance with EN ISO 8655 Technical specifications are subject to change without notice. 9. Quick Reference Guide Important: The Quick Reference Guide should only be used after the operator is thoroughly acquainted with the detailed instructions, otherwise errors may occur. Look for desired volume in the volume selection chart. (See page 5.) Select appropriate repeating tip by number of dispensing steps. Set volume selection dial to corresponding position. Slide filling lever down. Raise locking clamp upward. Insert tip into position. Lower locking clamp and close it completely. Immerse the tip into the liquid. Slide filling lever upward. 14 Discard first dispensing step. Dispense. Empty tip completely. Raise locking clamp upward. Remove tip. 10. Manufacturing and Inspection Standards Product Description Manual Repeating Pipette Catalog Number 4736-8025 This product has been manufactured and inspected in accordance with the following International and European standards: EN ISO 8655 ASTM E1154-89 (2003) 98/79/EEC Piston-Operated Volumetric Apparatus Standard Specification for Piston or Plunger Operated Volumetric Apparatus In Vitro Diagnostic Medical Devices August 2017 15Corel PDF Engine Version 18.1.0.661