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Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Page: 1/15 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier REF Product name 740982.10 NucleoSpin totalRNA FFPE (10) REACH Registration number(s): see SECTION 3.1/3.2 or A registration number for the substance(s) does not exist because the annual tonnage does not require registration or the substance or its use is excluded from registration. 1 x 7 mL DRB (OEM) 1 x 600 �L Liquid Proteinase K 1 x 1 mL MKA 1 x 10 mL MLF 1 x 6 mL MW2 1 x 13 mL MX 1 x 15 mL Paraffin Dissolver (blau) 1 x 200 U, Size D, rDNase 1 x 13 mL RNase-free H2 O 1.2 Relevant identified uses of the substance or mixture and uses advised against Relevant identified uses Product for analytical use. Exposure Scenario Classification according REACh, RIP 3.2 Codes: SU 0-2, PC 21, PROC 15, AC 0 The exposure scenario is integrated into sections 1-16. Uses advised against not described 1.3 Details of the supplier of the safety data sheet Manufactured by: MACHEREY-NAGEL GmbH & Co. KG Neumann-Neander-Str. 6-8, 52355 Dueren, GERMANY Tel.: +49 2421 969 0 E-mail: sds@mn-net.com (msds@mn-net.com) 1.4 Emergency telephone number Outside Germany (DE): Call your regional Poisons Information Service or call local Life Saving Service. DE: Gemeinsames Giftinformationszentrum (GGIZ) 99089 Erfurt tel. +49 361 730 730 You find our current versions of SDS (22 languages) in Internet: http://www.mn-net.com/SDS SECTION 2: Hazard identification 2.0 Classification of the complete product Signal word Hazard identification H225 H304 H319 H334 H335 H351 GHS02 GHS07 GHS08 DANGER Hazard classes/categories Flam. Liq. 2 Asp. Tox. 1 Eye Irrit. 2 Resp. Sens. 1 STOT SE 3 Carc. 2 Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 2.1 Classification of the substance or mixture 7 mL DRB (OEM) Signal word Do not need labelling as hazardous - No hazard class 600 �L Liquid Proteinase K Signal word No hazard class Do not need labelling as hazardous - 1 mL MKA Signal word No hazard class Do not need labelling as hazardous - 10 mL MLF Signal word No hazard class Do not need labelling as hazardous - 6 mL MW2 Signal word No hazard class Do not need labelling as hazardous - 13 mL MX Signal word Hazard identification H225 H319 H335 H351 GHS02 GHS07 DANGER GHS08 Hazard classes/categories Flam. Liq. 2 Eye Irrit. 2 STOT SE 3 Carc. 2 15 mL Paraffin Dissolver (blau) Signal word Hazard identification H304 GHS08 DANGER Hazard classes/categories Asp. Tox. 1 Version: M V 4.10.1 Page: 2/15 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 200 U, Size D, rDNase Page: 3/15 Signal word Hazard identification H334 GHS08 DANGER Hazard classes/categories Resp. Sens. 1 13 mL RNase-free H2 O Signal word No hazard class Do not need labelling as hazardous - 2.2 Label elements According CLP directive inner packages must be only labelled with GHS symbol(s) and product identificator(s) (EU 1272/2008 Annex I - 1.5.1.2). Harmful chemicals/mixtures with signal word: WARNING and highly flammable chemicals/mixtures must not be labelled with H and P phrases until 125 mL (EU 1272/2008 Annex I - 1.5.2). This labelling exemption is NOT valid for sensibilizing substances. 7 mL DRB (OEM) Do not need labelling as hazardous Signal word: - 600 �L Liquid Proteinase K Do not need labelling as hazardous Signal word: - 1 mL MKA Do not need labelling as hazardous Signal word: - 10 mL MLF Do not need labelling as hazardous Signal word: - 6 mL MW2 Do not need labelling as hazardous Signal word: - 13 mL MX GHS02 GHS07 GHS08 Signal word: DANGER H351 Suspected of causing cancer. P280sh Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Wear protective gloves/eye protection. 15 mL Paraffin Dissolver (blau) Page: 4/15 GHS08 Signal word: DANGER H304 May be fatal if swallowed and enters airways. P301+310, P331 IF SWALLOWED: Immediately call a POISON CENTER/ doctor. Do NOT induce vomiting. 200 U, Size D, rDNase GHS08 Signal word: DANGER H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled. P261sh, P342+311 Avoid breathing dust/vapours. If experiencing respiratory symptoms: Call a POISON CENTER/doctor. 13 mL RNase-free H2 O Do not need labelling as hazardous Signal word: - 2.3 Other hazards Possible hazards from physicochemical properties In the case of pH values are less than 5 or higher than 9 then it is irritant. Flammable properties. --- Information pertaining to particular risks to human and possible symptoms Cause after inhalation of vapours/dust, impairments of health when ingested in small quantities. Kit contains small amounts of enzymes: May cause allergy or asthma symptoms or breathing difficulties if inhaled. In liquid form no hazard H334. Suspected of causing cancer. May be fatal if swallowed and enters airways. The Paraffin Dissolver needs no labeling H350, because the aromates are reduced (<0.03%). - Information pertaining to particular risks to the environment PBT: not applicable vPvB: not applicable Other hazards --- SECTION 3: Composition/information on ingredients 3.1 Substances or 3.2 Mixtures 7 mL DRB (OEM) Chemical: chemicals/mixture < 2% Classification: No criteria for classification or naming of chemical not required. TSCA Inventory: all listed, <2% KE No.: listed Concentration: 1 - <2 % acc. CLP (GHS): The criteria for classification are not fulfilled. CAS No.: - Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 600 �L Liquid Proteinase K Chemical: proteinase K, liquid (origin: tritirachium album) CAS No.: 39450-01-6l Classification: H315, Skin Irrit. 2, H319, Eye Irrit. 2, H334, Resp. Sens. 1 Formula: Enzyme Comm. No. 3.4.21.64,origin: tritirachium album TSCA Inventory: listed (CAS 102925-54-2) EC No.: 254-457-8 Indice No.: 647-014-00-9 RTECS: - MFCD: 00132129 KE No.: not listed Concentration: 1 - <3 % acc. CLP (GHS): The criteria for classification are not fulfilled. Chemical: glycerole Classification: No criteria for classification or naming of chemical not required. Formula: C3 H8 O3 Pseudonym: glycerin, 1,2,3-propanetriol TSCA Inventory: listed (1,2,3-Propanetriol) REACH Reg. No.: 01-2119471987-18-xxxx EC No.: 200-289-5 Indice No.: RTECS: MA8050000 MFCD: KE No.: KE-29297 Concentration: 50 - <80 % acc. CLP (GHS): The criteria for classification are not fulfilled. CAS No.: 56-81-5 n/a 00004722 1 mL MKA Chemical: acetate buffer solution Classification: No criteria for classification or naming of chemical not required. Formula: CH3 COOH/K/Na�H2 O TSCA Inventory: all listed KE No.: listed Concentration: 45 - <60 % acc. CLP (GHS): The criteria for classification are not fulfilled. CAS No.: - 10 mL MLF Chemical: tris(hydroxymethyl)aminomethane Classification: No criteria for classification or naming of chemical not required. Formula: C4 H11 NO3 Pseudonym: Trometamol, 2-amino-2-(hydroxymethyl)-1,3-propanediol TSCA Inventory: listed REACH Reg. No.: 01-2119957659-16-0014 EC No.: 201-064-4 RTECS: TY2900000 MFCD: KE No.: KE-01403 Concentration: 1 - <3 % acc. CLP (GHS): The criteria for classification are not fulfilled. CAS No.: 77-86-1 00004679 6 mL MW2 Chemical: chemicals/mixture < 1% Classification: No criteria for classification or naming of chemical not required. TSCA Inventory: all listed, <1% KE No.: listed Concentration: 0.1 - <1 % acc. CLP (GHS): The criteria for classification are not fulfilled. CAS No.: - 13 mL MX Chemical: 1,4-dioxane CAS No.: 123-91-1 Classification: H225, Flam. Liq. 2, H319, Eye Irrit. 2, H335, STOT SE 3, H351, Carc. 2 Formula: C4 H8 O2 Pseudonym: diethylene dioxide; diethylene ether; p-dioxane TSCA Inventory: listed REACH Reg. No.: 01-2119462837-26-0001 EC No.: 204-661-8 Indice No.: 603-024-00-5 RTECS: JG8225000 MFCD: 00006571 KE No.: KE-10463 Concentration: 90 - <100 % acc. CLP (GHS): H225, Flam. Liq. 2, H319, Eye Irrit. 2, H335, STOT SE 3, H351, Carc. 2 Page: 5/15 Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 15 mL Paraffin Dissolver (blau) Chemical: paraffin dissolver (, Aromates <0.03%) Classification: H304, Asp. Tox. 1 Formula: C15 -C20 , isomere aliphates Pseudonym: distillates (petroleum), hydrotreated middle TSCA Inventory: listed REACH Reg. No.: 01-2119827000-58-XXXX EC No.: 934-956-3 Indice No.: KE No.: KE-12554 Concentration: 90 - <100 % acc. CLP (GHS): H304, Asp. Tox. 1 Chemical: colour dye(s) Classification: No criteria for classification or naming of chemical not required. TSCA Inventory: all listed, <1% Concentration: < 0.10 % acc. CLP (GHS): The criteria for classification are not fulfilled. CAS No.: 64742-46-7 649-275-00-4 CAS No.: - Page: 6/15 200 U, Size D, rDNase Chemical: rDNase Classification: H334, Resp. Sens. 1 Formula: Enzyme Comm. No. 3.1.21.1,origin: cloned Pseudonym: Nuclease, deoxyribo- TSCA Inventory: listed EC No.: 232-667-0 RTECS: RF0750000 KE No.: KE-09612 Concentration: 90 - <100 % acc. CLP (GHS): H334, Resp. Sens. 1 CAS No.: 9003-98-9 13 mL RNase-free H2 O Chemical: water Classification: Formula: TSCA Inventory: REACH Reg. No.: EC No.: RTECS: KE No.: Concentration: acc. CLP (GHS): No criteria for classification or naming of chemical not required. H2 O listed exempt, Annex IV 231-791-2 ZC0110000 KE-35400 90 - <100 % The criteria for classification are not fulfilled. CAS No.: 7732-18-5 3.3 Remarks When not listed, mixtures are added with water [CAS No. 7732-18-5] to 100%. List of H and P phrases: see section 16.1 SECTION 4: First aid measures 4.1 4.1.1 4.1.2 4.1.3 Description of first aid measures Place insured person out of danger zone to fresh air immediately. Ensure quiet, warmth, and provide resuscitation if necessary. If necessary contact medical advice. Take to a doctor, in a raised position if there are breathing difficulties. After SKIN Contact Remove contaminated clothing. Rinse the affected skin or mucous membrane thoroughly under running water. (If possible) use soap. After EYE Contact After contact with the eyes rinse thoroughly under running water with the eyelid wide open with eye washing bottle, eye douche or running water (protect intact eye). After INHALATION of vapours After inhalation of foam or vapour fresh air should be inhaled. Keep airways free. Administer a Dexamethasone spray as soon as possible. Ensure quiet, warmth, and provide resuscitation if necessary. In the event of respiratory distress ensure that the patient inhales oxygen. Secure the breathing, heart and circulatory function. --- Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Page: 7/15 4.1.4 4.2 4.3 After ORAL Intake After oral intake lots of water should be drunk after it has been ingested. --- Most important symptoms and effects, both acute and delayed Chronic Effects: May cause sensitization by skin contact, also in repeated contact of small amounts. May cause allergy or asthma symptoms or breathing difficulties if inhaled. CMR Effects: Suspected of causing cancer. --- Indication of any immediate medical attention and special treatment needed Inform patient respectively further measures and the possibility of long-term damages. --- SECTION 5: Firefighting measures 5.1 Extinguishing media Fire extinguishers appropriate to the fire classification, and, if applicable, a fire blanket must be available in a prominent location in the work area. All extinguishers like FOAM, WATER SPRAY, DRY POWDER, CARBON DIOXIDE can be used. 5.2 Special hazards arising from the substance or mixture DANGER: Highly flammable (GHS regulation). Forms explosive vapour-air mixtures. Formation of hazardous and caustic vapour-air mixtures possible. --- 5.3 Advice for firefighters No, for listed product. Product package burns like paper or plastic. 5.4 Additional information --- SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Do not breathe vapours. Wear suitable protective gloves (see 8.2.2). Regular staff training is necessary, indicating hazards and precautions on the basis of operating instructions. Restrictions on activity must be observed. 6.2 Environmental precautions not necessary 6.3 Methods and material for containment and cleaning up Bind any escaping liquid with inert absorbent. And dispose in accordance to local regulations for the disposal of hazardous chemicals. Clean any contaminated equipment and floors with plenty of water. Collect small amounts of leaked liquid and flush with water into drains. Not for organic solvents (see section 13). 6.4 Reference to other sections --- SECTION 7: Handling and storage 7.1 7.2 7.2.1 7.3 Precautions for safe handling Handling in accordance with the test instruction, that comes with the product. Use only in well-ventilated working areas. Conditions for safe storage, including any incompatibilities The original product package of MACHEREY-NAGEL allows a safe storage. Storage class (VCI): 3 Water hazard class (DE): 2 Requirements for stock rooms and containers Keep original product packages tightly closed during handling and storage. Specific end use(s) Product for analytical use. SECTION 8: Exposure controls/personal protection 8.1 Control parameters 7 mL DRB (OEM) Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Chemical: chemicals/mixture < 2% CAS No.: - 600 �L Liquid Proteinase K Chemical: proteinase K, liquid (origin: tritirachium album) SUVA(CH) MAK value: 0,0000615min mg/m� CAS No.: 39450-01-6l Chemical: glycerole DNEL: [inh] 56 mg/m� DNEL = Derived No-Effect Level (for workers) CAS No.: 56-81-5 PNEC(fresh water) : 0.885 mg/L PNEC = Predicted No Effected Concentration TRGS 900 (DE): 200 E mg/m� E/e respirable Short-term exposure factor: 2 (I), Y skin resorptive (H), respiratory sensitizable (Sa), skin sensitizable (Sh), teratogenic (Z) not securely excluded / (Y) certainly excluded SUVA(CH) MAK value: 50 e* mg/m� 1 mL MKA Chemical: acetate buffer solution CAS No.: - 10 mL MLF Chemical: tris(hydroxymethyl)aminomethane DNEL: [derm] 166.7 mg/kg bw/day; [inh] 117.5 mg/m� DNEL = Derived No-Effect Level (for workers) CAS No.: 77-86-1 PNEC(fresh water) : no data mg/L PNEC = Predicted No Effected Concentration NIOSH: not listed [TWA] Time-weighted average to a reference period of 8 hours, [STEL] Short-term exposure limit related to a 15-minute period OSHA: not listed 6 mL MW2 Chemical: chemicals/mixture < 1% CAS No.: - 13 mL MX Chemical: 1,4-dioxane DNEL: 73 mg/m� DNEL = Derived No-Effect Level (for workers) CAS No.: 123-91-1 PNEC(fresh water) : 10 mg/L PNEC = Predicted No Effected Concentration EU value: TRGS 900 (DE): 20 ppm / 73 mg/m� 20 ppm / 73 mg/m� E/e respirable Short-term exposure factor: 2 (I), H, Y skin resorptive (H), respiratory sensitizable (Sa), skin sensitizable (Sh), teratogenic (Z) not securely excluded / (Y) certainly excluded SUVA(CH) MAK value: TRGS 903 (DE): 20 ppm / 72 mg/m� 2-Hydroxyethoxyessigs�ure U/b Kreatinin 400 mg/g B blood, U urine, a no limitation, b end of exposition or shift NIOSH: Occupational Carcinogen List Yes; TWA30min 1 ppm / 3.6 mg/m� [TWA] Time-weighted average to a reference period of 8 hours, [STEL] Short-term exposure limit related to a 15-minute period OSHA: [skin] TWA 100 ppm / 360 mg/m� 15 mL Paraffin Dissolver (blau) Chemical: paraffin dissolver TRGS 900 (DE): 600 mg/m� E/e respirable CAS No.: 64742-46-7 Short-term exposure factor: 2 (II) skin resorptive (H), respiratory sensitizable (Sa), skin sensitizable (Sh), teratogenic (Z) not securely excluded / (Y) certainly excluded NIOSH STEL: OSHA: 10 mg/m� 5 mg/m� Chemical: colour dye(s) CAS No.: - 200 U, Size D, rDNase Page: 8/15 Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Chemical: rDNase CAS No.: 9003-98-9 Page: 9/15 13 mL RNase-free H2 O Chemical: water CAS No.: 7732-18-5 8.2 8.2.1 8.2.2 8.2.3 8.2.4 8.2.5 Exposure controls Good ventilation and extraction system in the room, floor resistant to chemicals with floor drainage and washing facilities. The highest level of cleanliness must be maintained at the workplace. Respiratory protection Use for open access of these substances for example a protection filter, class A/AX. No additional recommendations. Hand protection Yes, gloves according EN 374 (permeation time >30 min - level 2), consist of PVC, natural latex, Neopren, or Nitril (f.ex. from Ansell or KCL). Use for short times chemical resistant latex gloves with code EN 374-3 level 1. Eye protection Yes, safety glasses according EN 166 with integrated side shields or wrap-around protection. Skin protection Recommended to avoid contamination with these hazards. Personal hygiene Eating, drinking, smoking, taking snuff and storage of food in work areas and at outdoor workplaces is prohibited. Avoid contact with the skin, eyes and clothing. Rinse any clothing on which the substance has been spilled, and soak it in water. Wash hands thoroughly with soap and water when stopping work and before eating, and then apply protective skin cream. SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties 7 mL DRB (OEM) Appearance: liquid pH: Specific gravity: Colour: colourless 6.5-7.5 1.01 g/cm� Odor: odorless 600 �L Liquid Proteinase K Appearance: liquid pH: Specific gravity: Solubility in water: Colour: colourless 7-8 1.1 g/cm� 0-100 % Odor: odorless 1 mL MKA Appearance: liquid pH: Specific gravity: Colour: colourless 5-6 1.19 g/cm� Odor: acetic 10 mL MLF Appearance: liquid Solubility in water: Colour: colourless 0-100 % Odor: odorless 6 mL MW2 Appearance: liquid pH: Specific gravity: Colour: colourless 7-8 1.00 g/cm� Odor: odorless 13 mL MX Appearance: liquid Odor limit: pH: Melting point: Boiling point: Flash point: Evaporation rate(ether=1) : Explosion limits: Vapour pressure (20�C): Vapour density(air=1) : Specific gravity: Colour: colourless 10-620 mg/m� 6-8 12 �C 101.5 �C 11 �C 7,3 1.9-22.5 Vol% 41 hPa 3,04 1.01-1.03 g/cm� Odor: odorless Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Page: 10/15 Solubility in water: Flashing temperature: Volatiles by volume: < 2 % 375 �C 149 g/m� 15 mL Paraffin Dissolver (blau) Appearance: liquid pH: Melting point: Boiling point: Flash point: Evaporation rate(ether=1) : Explosion limits: Vapour pressure (20�C): Specific gravity: Solubility in water: Dispersion coefficient(octanol-water) : Flashing temperature: Colour: blue -18pourpoint �C 245-330 �C >115 �C >1000 1-6 Vol% < 0.003 hPa 0.82 g/cm� <0,001 % 7-8.7 >230 �C Odor: organic 200 U, Size D, rDNase Appearance: solid (lyoph.) Colour: white Odor: odorless 13 mL RNase-free H2 O Appearance: liquid pH: Specific gravity: Colour: colourless 6-8 1.0 g/cm� Odor: odorless 9.2 Other information Data for the other parameters of the mixtures are not available, because no registration and no chemical safety report is required. Relevant Properties of Substance Group Substances are very volantile and form flammable vapour-air mixtures. --- SECTION 10: Stability and reactivity 10.1 10.2 10.3 10.4 10.5 10.6 Reactivity no further data available. Chemical stability No known instability. Possibility of hazardous reactions No further data available. Conditions to avoid Not necessary. --- Incompatible materials Avoid contact with strong acids or alkalines. Hazardous decomposition products In the original package all parts/all reagents are safety and separated stored. Decompositions are not observed during the expiration period under recommended conditions. SECTION 11: Toxicological information 11.1 Information on toxicological effects Following information is valid for pure substances. Quantitative data on the toxicity of this product are not available. 7 mL DRB (OEM) Chemical: chemicals/mixture < 2% TSCA Inventory: all listed, <2% Korea Exist.Chem.Inventory: listed CAS No.: - Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 600 �L Liquid Proteinase K Chemical: proteinase K, liquid (origin: tritirachium album) TSCA Inventory: listed (CAS 102925-54-2) Japan CSCL/PRTR: not listed Japan ISHL: not listed Korea Exist.Chem.Inventory: not listed CAS No.: 39450-01-6l Chemical: glycerole CAS No.: 56-81-5 TSCA Inventory: listed (1,2,3-Propanetriol) Exposure Routes: inhalation, skin and/or eye contact Target Organs: Eyes, skin, respiratory system, kidneys Symptoms: irritation eyes, skin, respiratory system; headache, nausea, vomiting; kidney injury Japan CSCL/PRTR: not listed, Japan PDSCL: not listed Japan ISHL: not listed Korea Exist.Chem.Inventory: KE-29297 LD50orl rat : 12.6 g/kg LD50drm rbt : >18.7 g/kg TRGS 905 (DE): RF C 1 mL MKA Chemical: acetate buffer solution TSCA Inventory: all listed Korea Exist.Chem.Inventory: listed CAS No.: - Page: 11/15 10 mL MLF Chemical: tris(hydroxymethyl)aminomethane CAS No.: 77-86-1 TSCA Inventory: listed California Proposition 65 List: not listed Australia NICNAS: not listed Canada CEPA 1999: DSL yes Japan CSCL/PRTR: not listed, Japan PDSCL: not listed Japan ISHL: not listed South Korea TCCA: not listed Korea Exist.Chem.Inventory: KE-01403 LD50orl rat : 5000 mg/kg 6 mL MW2 Chemical: chemicals/mixture < 1% TSCA Inventory: all listed, <1% Korea Exist.Chem.Inventory: listed CAS No.: - 13 mL MX Chemical: 1,4-dioxane CAS No.: 123-91-1 TSCA Inventory: listed California Proposition 65 List: listed cancer Exposure Routes: inhalation, skin absorption, ingestion, skin and/or eye contact Target Organs: Eyes, skin, respiratory system, liver, kidneys; [in animals: lung, liver & nasal cavity tumors] Symptoms: irritation eyes, skin, nose, throat; drowsiness, headache; nausea, vomiting; liver damage; kidney failure; [potential occupational carcinogen] Australia NICNAS: Yes (PEC/7) Canada CEPA 1999: DSL Yes Japan CSCL/PRTR: PAC Yes, PRTR - Class I Designated Chemical SubstanceYes, Japan PDSCL: PRTR: 1,0% class I Japan ISHL: listed 1,0%/1,0%, Article 57-1+2 (Labelling&SDS required) South Korea TCCA: not listed Korea Exist.Chem.Inventory: KE-10463 LD50orl rat : 5150 mg/kg LC50ihl rat : [LC0, 60min] 155 mg/L Acute Effects: Cause after inhalation of vapours/dust, impairments of health when ingested in small quantities. Carcinogenic Effects: Suspected of causing cancer. EU carcinogen: Carcinogenicity cat. 2 TRGS 905 (DE): K4, RF C Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 15 mL Paraffin Dissolver (blau) Chemical: paraffin dissolver TSCA Inventory: listed Japan CSCL/PRTR: not listed, Japan PDSCL: not listed Japan ISHL: not listed Korea Exist.Chem.Inventory: KE-12554 LD50orl rat : >5000 mg/kg LC50inh rat : >52664h mg/m� LD50drm rbt : >3160 mg/kg CAS No.: 64742-46-7 Chemical: TSCA Inventory: colour dye(s) all listed, <1% CAS No.: - Page: 12/15 200 U, Size D, rDNase Chemical: rDNase CAS No.: 9003-98-9 TSCA Inventory: listed Japan CSCL/PRTR: not listed Japan ISHL: not listed Korea Exist.Chem.Inventory: KE-09612 Acute Effects: Cause after impairments of health when ingested in small quantities. Chronic Effects: May cause sensitization by skin contact, also in repeated contact of small amounts. May cause allergy or asthma symptoms or breathing difficulties if inhaled. 13 mL RNase-free H2 O Chemical: water TSCA Inventory: listed Korea Exist.Chem.Inventory: KE-35400 CAS No.: 7732-18-5 SECTION 12: Ecological information 12.1 Toxicity Following information is valid for pure substances. 7 mL DRB (OEM) Chemical: chemicals/mixture < 2% Water hazard class (DE): 1 Storage class (VCI): 12-13 600 �L Liquid Proteinase K Chemical: proteinase K, liquid (origin: tritirachium album) Water hazard class (DE): 1 Storage class (VCI): 13 Chemical: glycerole PNEC(fresh water) : 0.885 mg/L PNEC = Predicted No Effected Concentration LC50fish/96h : EC50daphnia/48h : IC50scenedesmus quadricauda/72h : EC10pseudomonas putita/16h : Water hazard class (DE): Dispersion coefficient(octanol-water) : Storage class (VCI): >500024h mg/L >1024h g/L IC57d >10 g/L EC5: >10 g/L 0 -1.76 10 1 mL MKA Chemical: acetate buffer solution Storage class (VCI): 12 10 mL MLF CAS No.: CAS No.: 39450-01-6l CAS No.: 56-81-5 CAS No.: - Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Chemical: tris(hydroxymethyl)aminomethane PNEC(fresh water) : no data mg/L PNEC = Predicted No Effected Concentration LC50fish/96h : LD0 (4d): 1-10 g/L EC50pseudokirchneriella subcapitata/72h : 397;48h: 473 mg/L EC10pseudomonas putita/16h : 13h g/L Water hazard class (DE): 2 Dispersion coefficient(octanol-water) : -1.56 Storage class (VCI): 12-13 CAS No.: 77-86-1 Page: 13/15 6 mL MW2 Chemical: chemicals/mixture < 1% Water hazard class (DE): 1 Storage class (VCI): 12-13 CAS No.: - 13 mL MX Chemical: 1,4-dioxane PNEC(fresh water) : 10 mg/L PNEC = Predicted No Effected Concentration Bio Toxicity: LC50fish/96h : EC50daphnia/48h : IC50scenedesmus quadricauda/72h : Water hazard class (DE): Dispersion coefficient(octanol-water) : Storage class (VCI): 1/2.1/2.6 [21d] 100 mg/L 1 g/L [72h] 1 g/L 2 WGK No.: 0086 -0.42 3 CAS No.: 123-91-1 15 mL Paraffin Dissolver (blau) Chemical: paraffin dissolver LC50fish/96h : >1028 mg/L Water hazard class (DE): 1 WGK No.: 0027 Dispersion coefficient(octanol-water) : 7-8.7 Storage class (VCI): 12 Chemical: colour dye(s) CAS No.: 64742-46-7 CAS No.: - 200 U, Size D, rDNase Chemical: rDNase Water hazard class (DE): Storage class (VCI): 1 WGK No.: n.n. 13 CAS No.: 9003-98-9 13 mL RNase-free H2 O Chemical: water CAS No.: 7732-18-5 12.2 12.3 12.4 12.5 12.6 Persistence and degradability not necessary Bioaccumulative potential not necessary Mobility in soil not necessary Results of PBT and vPvB assessment no data available Other adverse effects no additional data available Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Page: 14/15 SECTION 13: Disposal considerations Please observe local regulations for collection and disposal of hazardous waste and contact waste disposal company, where you will obtain information on laboratory waste disposal (waste code number 16 05 06). Or collect in solvent waste (waste code number 07 07 04). 13.1 Waste treatment methods Normally it is possible to empty small amounts (diluted!) into drains. SECTION 14: Transport information UN 1993 class 3 II, Excepted Quantities (30 mL/500 mL) = ADR/ IATA E2 or 14.1 UN number: 1993 14.2 UN proper shipping name: Flammable liquid, n.o.s. (1,4-dioxane mixture) 14.3 Class: 3 14.4 Packing group: II Road transport Classification code: F1 Limited Quantity: 1 L Tunnel restriction code: E Excepted Quantity: E 2 Special instructions: 640C Air transport PAX: 353 max. weight PAX: 5 L CAO: 364 max. weight CAO: 60 L Maritime transport EmS: F-E, S-E Storage category: B 14.5 14.6 14.7 Environmental hazards none, contains only small quantities of hazardous substances Special precautions for user not necessary Transport in bulk according to Annex II of MARPOL and the IBC Code not applicable SECTION 15: Regulatory information 15.1 15.2 Safety, health and environmental regulations/legislation specific for the substance or mixture German act governing protection from hazardous substances (Chemicals Act / Chemikaliengesetz- ChemG), revised on August 2013 German order governing protection from hazardous substances (Ordinance on Hazardous Substances / Gefahrstoffverordnung GefStoffV), revised on November 2010, according to Directive 98/24/EC TRGS 200, German engineering rules governing the classification and labelling of hazardous substances, preparations and products, updated October 2011 MN Leaflet/User manual, also see www.mn-net.com Look for your country-specific regulations. Chemical safety assessment not necessary for these small amounts --- SECTION 16: Other information 16.1 16.1.1 16.1.2 List of H and P phrases List of relevant H phrases H225 Highly flammable liquid and vapour. H304 May be fatal if swallowed and enters airways. H319 Causes serious eye irritation. H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled. H335 May cause respiratory irritation. H351 Suspected of causing cancer. List of relevant P phrases P201 Obtain special instructions before use. P210 Keep away from heat/sparks/open flames/hot surfaces. No smoking. P233 Keep container tightly closed. P261sh Avoid breathing dust/vapours. P280sh Wear protective gloves/eye protection. P301+310 IF SWALLOWED: Immediately call a POISON CENTER/ doctor. Version: M V 4.10.1 Safety Data Sheet according to Regulations 1907/2006/EC (REACh) and 2015/830/EU REF: 740982.10 Printing date: 28.05.2020 NucleoSpin totalRNA FFPE (10) Date of issue: 24.06.2019 Page: 15/15 16.2 16.3 16.4 16.5 P304+340 P331 P342+311 P403+233 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Do NOT induce vomiting. If experiencing respiratory symptoms: Call a POISON CENTER/doctor. Store in a well-ventilated place. Keep container tightly closed. Training advice Multiple safety training of staffs about danger and protection by using hazards in working area. Additionally training and introduction of staffs for using these products. Recommended restriction on use Only for professional user. Look about employee restrictions for young people (f. ex. 94/33/EC or DE � 22 JArbSchG)! Look about employee restrictions for pregnant women and nursing women (f.ex. 92/85/EEC or for DE �� 11-13 MuSchG 2017)! An individual package of this product or test kit has a moderate hazardous potential. Further information MACHEREY-NAGEL GmbH & Co. KG provides the information contained herein in good faith being up-to-date of own realizations at revision time. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Individuals receiving the information must exercise their independent judgement in determining its appropriateness for a particular purpose. MACHEREY-NAGEL GmbH & Co. KG makes NO REPRESENTATIONS or WARRANTIES, either expressed or implied, including without limitation any warranties of merchantability, fitness for a particular purpose with respect to the information set forth herein or the product to which the information refers. Accordingly MACHEREY-NAGEL GmbH & Co. KG will not be responsible for damages resulting from use of or reliance upon this information. See terms and conditions at the end of our price lists for additional information. Sources of key data Regulation 453/2010/EU REACH - REQUIREMENTS FOR THE COMPILATION OF SAFETY DATA SHEETS Regulation 487/2013/EU, 4th adaptation of CLP regulation to technical and scientific progress Regulation 669/2018/EU, 4th adaptation of CLP regulation to technical and scientific progress Regulation 1480/2018/EU, 4th adaptation of CLP regulation to technical and scientific progress TRGS 900, German engineering rules governing limits in air at work, updated 03/2019 SUVA .CH, Limits in air at work 2009, revised on 01.2009 TRGS 907, German engineering rules governing listing of substances and causes of sensitizations, updated November 2011 K�HN, BIRETT Merkbl�tter Gef�hrliche Arbeitsstoffe (Data Sheets of Hazardous Substances) Revisions/Updates Reason for Revision: 2016-03 Adaptation of regulation 1221/2015/EU Version: M V 4.10.1