LABORATORY RESEARCH SERVICES Skeletal Muscle Cells & Media Human muscle derived cells isolated and cultured using Cook MyoSite's process were stained with a Mouse Anti-Human Desmin antibody Clone D33 (Dako M0760) at 1:250. Secondary antibody was Sheep anti-mouse::FITC (Sigma F2883) at 1:250. Nuclei were stained with DAPI. Invest in high-quality primary cells and media to make your research more reliable, consistent, and efficient. Cook MyoSite® offers single-donor primary human skeletal muscle cells (skMDC) characterized by age, gender, race, and BMI with specially formulated media that is optimized to make cell culture growth, differentiation, and cryopreservation as reliable as possible in your lab. We offer healthy and diseased cells from a range of donors, and we can generate gene expression profiles for each lot of cells to further characterize expression levels of myogenic gene targets. When you purchase cells or media, you're purchasing our expertise, too. At Cook MyoSite, our services are backed by nearly two decades of clinical experience in muscle-related research. skMDC and media are processed using only the highest quality materials, and our highly experienced Laboratory Research Services team provides expert customer service and technical support to ensure that the cells perform as expected. www.cookmyosite.com skMDC Human Skeletal Muscle Cells Our skMDC are myoblast-like, non-differentiated cells for Research Use Only (RUO). Each lot must pass an assortment of quality tests to be approved for shipment including the ability to differentiate unless the donor has been clinically diagnosed with a condition that might affect this ability. We expect 8-12 population doublings from 1 Standard Donor vial or potentially more if flask confluence is kept per our IFU specifications. 1 vial contains 500,000 cells. Upon request, we can provide gene expression profiles, and in some cases we may be able to provide information regarding diabetes history, physical activity levels, and other such information. Contact Cook MyoSite for a complete listing of available lots and pricing Catalog # SK-1111 SK-2222 Description skMDC Human Skeletal Muscle Cells Standard Donors skMDC Human Skeletal Muscle Cells Rare & Neuromuscular Diseased Donors Volume MSRP 1mL Varies 1mL Varies MyoTonicTM Family of Culture Media Our MyoTonic Family of Culture Media is optimized for use with our skMDC cell samples and provides your lab with a range of options for cell growth, differentiation, and custom applications. Basal Medium Catalog # Description MB-2222 MyoTonic Basal Medium ML-6666 MyoTonic Insulin-Free Basal Medium Volume MSRP 450mL $98.18 450mL $102.51 Supplements Use our Growth Supplements to expand experimentally relevant quantities while maintaining culture phenotype. Catalog # MS-3333 MS-8888 Description MyoTonic Growth Supplement MyoTonic Serum-Free Growth Supplement Volume MSRP 50mL $185.13 3mL $133.88 Differentiation Our Differentiation Media to achieve consistent and controlled differentiation. Catalog # MD-5555 MD-9999 Description MyoTonic Differentiation Media MyoTonic Serum-Free Differentiation Media Volume MSRP 250mL $103.79 250mL $98.89 CryoTonicTM Cryostorage Medium Preserve your cells with our CryoTonic Cryostorage Medium, carefully formulated for mamallian cells to maintain high viability post-thaw. Catalog # CR-9999 Description CryoTonic Cryostorage Medium Volume MSRP 50mL $141.53 www.cookmyosite.com Publications Our skMDC cell samples and media have been featured in a number of publications around the world. We encourage you to look through these studies and see how our offerings have helped researchers in a variety of fields produce results worth publishing. Discounts are available for researchers who mention our products in their publications. Meltzer M, Long K, Nie Y, Gupta M, Yang J, Montano M. The RNA Editor Gene ADAR1 is Induced in Myoblasts by Inflammatory Ligands and Buffers Stress Response. Clinical and Translational Science. 2010;3(3):73-80 Kang Z, Chen JJ, Yu Y, Li B, Sun SY, et al. Drozitumab, a human antibody to death receptor 5, has potent antitumor activity against rhabdomyosarcoma with the expression of caspase-8 predictive of response. Clinical Cancer Research. 2011;17(10): 3181-92 Jankowski R. A comparison of commercially-available human skeletal muscle cells and media for research applications. Nature Methods/Application Notes 2011 Owens J, Moreira K, Bain G. Characterization of primary human skeletal muscle cells from multiple commercial sources. In Vitro Cellular & Developmental Biology Animal 2013;49(9): 695-705 Lee TY, Meszaros LB, Tobita K, Kumar AR. 3D Constructs of Human Skeletal Muscle-Derived Cells as a Model of Bone Formation: Osteogenic Differentiation in Response to BMP2. Plastic and Reconstructive Surgery 2013;132: 147-48 Lach-Trifilieff E, Minetti GC, Sheppard K, Ibebunjo C, Feige JN, et al. An antibody blocking activin type II receptors induces strong skeletal muscle hypertrophy and protects from atrophy. Molecular and Cellular Biology 2013;34(4): 606-18 Ye X, Lu L, Kolewe ME, Park H, Larson BL, et al. A biodegradable microvessel scaffold as a framework to enable vascular support of engineered tissues. Biomaterials 2013;34(38): 10007-15 Tchao J, Kim JJ, Lin B, Salama G, Lo CW, et al. Engineered Human Muscle Tissue from Skeletal Muscle Derived Stem Cells and Induced Pluripotent Stem Cell Derived Cardiac Cells. International Journal of Tissue Engineering 2013;2013: 198762 Tchao J, Han L, Lin B, Yang L, Tobita K. Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells. Scientific Reports 2014;4(1): 6614 Sousa-Victor P, Gutarra S, Garcia-Prat L, Rodriguez-Ubreva J, Ortet L, et al. Geriatric muscle stem cells switch reversible quiescence into senescence. Nature 2014;506: 316-21 Pessina P, Kharraz Y, Jardi M, Fukada S, Serrano AL, et al. Fibrogenic Cell Plasticity Blunts Tissue Regeneration and Aggravates Muscular Dystrophy. Stem Cell Reports 2015;4(6): 1046-60 Egerman MA, Cadena SM, Gilbert JA, Meyer A, Nelson HN, et al. GDF11 Increases with Age and Inhibits Skeletal Muscle Regeneration. Cell Metabolism. 2015;22(1): 164-74 Das S, Morvan F, Jourde B, Meier V, Kahle P, et al. ATP citrate lyase improves mitochondrial function in skeletal muscle. Cell Metabolism. 2015;21(6): 868-76 Duffy RM, Sun Y, Feinberg AW. Understanding the Role of ECM Protein Composition and Geometric Micropatterning for Engineering Human Skeletal Muscle. Annals of Biomedical Engineering 2016;44(6): 2076-2089 Summermatter S, Bouzan A, Pierrel E, et al. Blockade of Metallothioneins 1 and 2 Increases Skeletal Muscle Mass and Strength. Molecular and Cellular Biology. 2016;37(5) Brzeszczyska J, Meyer A, Mcgregor R, et al. Alterations in the in vitro and in vivo regulation of muscle regeneration in healthy ageing and the influence of sarcopenia. Journal of Cachexia, Sarcopenia and Muscle. 2017;9(1):93-105 Laternser S, Keller H, Leupin O, Rausch M, Graf-Hausner U, Rimann M. A Novel Microplate 3D Bioprinting Platform for the Engineering of Muscle and Tendon Tissues. SLAS Technol. 2018;23(6):599-613. doi:10.1177/2472630318776594 Vila OF, Uzel SGM, Ma SP, et al. Quantification of human neuromuscular function through optogenetics. Theranostics. 2019;9(5):12321246. doi:10.7150/thno.25735 Liqing L, Sherry CM, Jascha P, et al. HDAC4 Controls Muscle Homeostasis through Deacetylation of Myosin Heavy Chain, PGC-1, and Hsc70. Cell Reports. 2019;29(3):749-763.e12. doi:10.1016/j.celrep.2019.09.023. www.cookmyosite.com Product Use and Guidelines Cook MyoSite products are distributed for research use only. The use of these products is not approved for human or veterinary use. Do not use Cook MyoSite products with in vitro diagnostic assays. Products contain human source material. Treat as potentially infectious. Handle at Biological Safety Level 2 to minimize exposure to potentially infectious agents. Each donor tissue, prior to cell isolation, is tested for the presence of HIV, Hepatitis B, and Hepatitis C. Testing cannot offer complete assurance that tested viruses are absent. Buyer has no rights to transfer products, components or materials made using these products or use these products for commercial purposes. The term "commercial purposes" includes, but is not limited to, any of the following: use of products or their components in manufacturing; use of the product or their data components to provide a service, information, or data; intentional reproduction of cell products or their components indefinitely via immortalization; intentional use of products or their data components to intentionally identify individuals associated with applicable research samples; or use of products or their components for therapeutic or diagnostic purposes or resale of products or their components. Shipping Information Cook MyoSite ships products globally to areas including but not limited to Canada, Europe, the United Kingdom, China, Japan, South Korea, and Australia. We typically ship domestic orders within 5-7 business days and international orders within 7-14 days. Disclaimer Pricing may vary based on material sourcing and availability. Although the information in this skMDC Inventory List is presented in good faith and believed to be correct at the time of distribution, Cook MyoSite makes no representations or warranties as to the completeness or accuracy of the information. Cook MyoSite has no liability for any errors or omissions and maintains no obligation to update this listing. For more information please see our Terms and Conditions of Sale and Delivery or contact us. Cook MyoSite, Inc. 105 Delta Drive, Pittsburgh, PA 15238 Ph. 855.867.9368 | Fax. 888.456.4488 researchsupport@cookmyosite.com | www.cookmyosite.com ©2020 COOK. All Rights Reserved.