G1072 Agilent GC LC MS Catalog

User Manual: G1072

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GC AND GC/MS

250

GC AND GC/MS

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LC AND LC/MS

710

LC AND LC/MS

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In this Chapter
252

GC and GC/MS Maintenance Schedule

Parts and Supplies

254
280
318
337

GC General Supplies
GC Instrument Parts and Supplies
GC/MS Parts and Supplies
GC Parts and Supplies for Varian Instruments

GC and GC/MS Applications
568
617
641
671
686

Environmental Applications
Foods, Flavors and Fragrance Applications
Industrial Chemical Applications
Life Science Applications
Petroleum Applications

Agilent J&W GC and GC/MS Columns
359
362
364
392
430
442
461
524
531
532
559

Ultra Inert Columns
Guard Columns
Low-bleed GC/MS Columns
Polysiloxane Polymers Columns
Polyethylene Glycol (PEG) Columns
PLOT Columns
Special Application Columns
Packed GC Columns
Test Standards
Fused Silica Tubing
GC Column Troubleshooting and Maintenance

GC AND GC/MS

251

In this Chapter
Parts and Supplies
712
716
739
752
758
762
772
775
787

Bio-inert Supplies
General Supplies
Pump Supplies
Autosampler Supplies
Fraction Collector Supplies
Valve Supplies
TCC Supplies
Detector Supplies
LC/MS Supplies

LC and LC/MS Columns
796
806
818
891
916
945
996

HPLC Column Selection
Cartridge Column Systems
Columns for Analytical HPLC
Columns for Special Applications
Preparative HPLC Columns and Flash Chromatography
GPC/SEC Columns and Calibrants
Biosolutions and Columns for Biologic Characterization

LC and LC/MS Troubleshooting
1052 HPLC Troubleshooting
1056 LC/MS Troubleshooting

LC and LC/MS Applications
1059
1076
1079
1088
1102

BioPharmaceutical Applications
Chemical/Industrial Applications
Environmental Applications
Food and Consumer Product Applications
Pharmaceutical Applications

LC AND LC/MS

711

GC/MS Parts and Supplies

GC/MS Parts and Supplies
Your mass spectrometer is a sensitive, specialized device that delivers a higher level
of functionality than other GC detectors. To continue achieving optimal results, it is critical
to maintain your system properly by performing the essential tasks within this section.
Some of the benefits of maintaining your GC/MSD include:

• Less downtime for repairs
• Longer lifetime for your MSD system
• Reduction in overall operating costs
It is advisable to keep a log book of system performance, Autotune, and maintenance operations
performed. This makes it easier to identify variations from normal performance and to take
corrective action.

Maintenance Schedule
Task
Every week Every 6 months Every year As needed
Tune the MSD
]
Change injection port liners
]
Check the foreline pump oil level
]
Gas ballast the foreline pump
]
Check the calibration vial
]
Replace the foreline pump oil
]
Check the diffusion pump fluid
]
Replace the diffusion pump fluid
]
Replace the dry pump
]
diaphragm seals (MVP55)
Replace the dry pump tip seals (IDP3)
]
Replace the traps and filters
]
Clean the ion source
]
Change the carrier gas trap(s)
]
and purifier
Replace worn out parts
]
Lubricate seals (where appropriate)
]
Replace column
]

For in-depth information about maintaining your GC/MS, request "Maintaining Your Agilent GC
and GC/MS Systems" from your Agilent Representative (publication number 5990-5451EN).

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MSD Contamination

MSD Contamination
Contamination is usually identified by excessive background in the mass spectra, which can come
from the GC or MSD. The source of contamination can sometimes be determined by identifying
the contaminants. Some contaminants are much more likely to originate in the GC, while others
are likely to originate in the MSD.

MSD Contamination Identification
The following table lists some of the more common contaminants, the ion characteristics of those
contaminants, and the likely sources of those contaminants.

Tips & Tools
A crude sign of a "leak-free" MS
system is when the ion ratio of m/z
28 (nitrogen) over m/z 32 (oxygen)
is approximately two or greater.

Common Contaminants
Ions (m/z)
13, 14, 15, 16
18, 28, 32, 44 or 14, 16

Compound
Methane
H20, N2, O2, CO2, CO2 or N, O

31, 51, 69, 100, 119, 131,
169, 181, 214, 219, 264,
376, 414, 426, 464, 502,
576, 614
31
43, 58
78
91, 92
105, 106
151, 153
69

PFTBA and related ions

Methanol
Acetone
Benzene
Toluene or Xylene
Xylene
Trichloroethane
Foreline pump fluid or PFTBA

73, 147, 207, 221, 281, 295, Dimethylpolysiloxane
355, 429
77, 94, 115, 141, 168, 170, Diffusion pump fluid
262, 354, 446
149
Plasticizer (phthalates)

Peaks spaced 14 amu apart

Hydrocarbons

Possible Source
CI gas
Residual air and water,
air leaks, outgassing from
Vespel ferrules
PFTBA (tuning compound)

Cleaning solvent
Cleaning solvent
Cleaning solvent
Cleaning solvent
Cleaning solvent
Cleaning solvent
Foreline pump oil vapor
or calibration valve leak
Septum bleed or methyl silicone
column coating
Diffusion pump fluid
and related ions
Vacuum seals (O-rings)
damaged by high temperatures,
use of vinyl or plastic gloves
Fingerprints, foreline pump oil

PARTS AND SUPPLIES

319

MSD Contamination

Cleaning and Maintenance Supplies
Description
One Year Maintenance Kit (for diffusion pump systems)
Includes Big Universal Trap for He (1/8 in.), abrasive sheets (5/pk),
lint-free cloths (15/pk), cotton swabs (100/pk), SantoVac Ultra, 18.5 mL (2 each),
rough pump oil (1 L), filament assembly, octafluoronapthalene (OFN)
Nylon gloves, lint-free, large, 1 pair
Nylon gloves, lint-free, small, 1 pair
Lint-free industrial wipes, 100% cotton, 9 x 9 in., 300/pk
Ion source cleaning kit
Includes lint-free cloths (15/pk), abrasive sheets (5/pk), cotton swabs (100/pk),
lint-free nylon gloves, abrasive Alumina powder
Cloths, lint-free, 15/pk
Cotton swabs, 100/pk
Abrasive sheets, aluminum oxide green lapping paper, 600 mesh, 5/pk
Alumina powder, abrasive, 1 kg
PFTBA sample, certified, 10 g, 5.32 mL
Replacement glass bulb for PFTBA and PFDTD test sample
Replacement glass vial for PFTBA and PFDTD test sample
Activated alumina, absorbent pellets for Edwards rough pump traps,
non-LC/MS, 1 lb can
MSD Tool Kit, 5975/5973
Includes source hold tool, lint-free cloth, cotton swabs, lint-free nylon gloves,
abrasive sheets, wrenches and driving tools
MSD Tool Kit, 5972/5971
Includes small cleaning rod, large cleaning rod, source hold tool, cotton swabs,
lint-free nylon gloves, abrasive sheets, wrenches and driving tools

Part No.
5183-2096

8650-0030
8650-0029
9310-4828
5181-8863

05980-60051
5080-5400
5061-5896
8660-0791
8500-0656
G3170-80002
05980-20018
8500-1233
G1099-60566

05971-60561

MS Interface Supplies
MS interface column nut, 05988-20066

MS interface column nut, female
Inlet column nut for long or long two-hole ferrules
Universal column nut, 2/pk
MS interface column installation tool for 5973 and 5975
Column installation tool for 5975T

05988-20066
05921-21170
5181-8830
G1099-20030
G3880-20030
(Continued)

Universal column nut, 5181-8830

Column installation tool, G1099-20030

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MSD Contamination

Cleaning and Maintenance Supplies
Description

Part No.

Tools

Screwdriver, 3 in. Pozidriv shaft No. 1 pt, fits no. 2-4 screws
Screwdriver, 4 in. Pozidriv shaft No. 2 pt, fits no. 5-10 screws
Open end wrench, 1/4 and 5/16 in.
Hex nut driver, 5.5 mm
Screwdriver, Torx T20
Screwdriver, Torx T15
Screwdriver, Torx T10

8710-0899
8710-0900
8710-0510
8710-1220
8710-1615
8710-1622
5182-3466

Ferrules

Vespel/Graphite ferrules, 5181-3323

0.4 mm Vespel/Graphite ferrule for 200/250 µm columns, 10/pk
0.5 mm Vespel/Graphite ferrule for 320 µm columns, 10/pk
250 µm Vespel/Graphite ferrule, 10/pk
SilTite metal ferrules for 1/16 in. OD tubing, 10/pk
Includes 2 column nuts
SilTite metal ferrules, 1/16 in. x 0.4 mm ID, 10/pk
Includes 2 column nuts
SilTite metal ferrules, 1/16 in. x 0.5 mm ID, 10/pk
Includes 2 column nuts
Ferrule pre-swaging tool
Plug for microfluidic manifold or unions

5062-3508
5062-3506
5181-3323
5184-3571
5184-3569
5184-3570
G2855-60200
G2855-60570

Tips & Tools
Even preconditioned ferrules can shrink slightly at very high
temperatures. If leak problems persist upon a new column
installation, check this fitting first.

PARTS AND SUPPLIES

321

Ion Source

Ion Source

Electron Impact (EI) Ion Source

The ion source operates by electron ionization (EI) or chemical ionization (CI). The sample
enters the ion source from the GC/MSD interface. Electrons emitted by a filament enter
the ionization chamber, guided by a magnetic field. The high-energy electrons interact with
the sample molecules, ionizing and fragmenting them. The positive voltage on the repeller
pushes the positive ions into the lens stack, where they pass through several electrostatic lenses.
These lenses concentrate the ions into a tight beam, which is directed into the mass filter.

Electron Impact (EI) Ion Source
The recommended cleaning material for the EI ion source is abrasive, aluminum oxide powder.
Do not immerse filaments or lens insulators in solvent. If insulators are dirty, clean them with
a cotton swab dampened with reagent-grade methanol. If that does not clean the insulators,
replace them.

5975/5973 MSD Electron Impact Ion Source Parts (EI)
Item Description
1
Ion source body
2
Drawout plate, 3 mm
Drawout plate, 6 mm
3
Drawout cylinder
4
Ion focus lens
5
Lens insulator
6
Entrance lens
7
Cap screw, gold plated
8
High temperature filament
9
Transfer line socket
10
Repeller insulator
11
Repeller

Part No.
G1099-20130
05971-20134
G3163-20530
G1072-20008
05971-20143
G3170-20530
G3170-20126
G1999-20021
G2590-60053
G1099-20136
G1099-20133
G1099-20132

6
5

9

4
3

11

2

10

1

7

8

5975/5973 MSD Electron Impact (EI) ion source assembly

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Inert
Part No.
G2589-20043
G2589-20100
G2589-20045
G1072-20008
05971-20143
G3170-20530
G3170-20126
G1999-20021
G2590-60053
G1099-20136
G1099-20133
G2589-20044

Ion Source

Tips & Tools
It is good practice to replace
scratched lenses and other
ion source parts regularly.
Scratched source parts lead
to poor performance.

5972/5971/GCD MSD Ion Source Parts (EI)
Description
Entrance lens
Lens insulator
Ion focus lens
Drawout cylinder
Drawout plate, 3 mm
Set screw
Repeller assembly
Screw for filament on the source
Transfer line tip, gold plated

Part No.
05971-20126
G3170-20530
05971-20143
G1072-20008
05971-20134
0515-1446
05971-60170
0515-1046
05971-20305

Warnings & Caution
Important: Do not abrasively or ultrasonically clean
the insulators.
Abrasively clean the surfaces that contact the sample or
ion beam. Use an abrasive slurry of alumina powder and
reagent-grade methanol on a cotton swab. Use enough
force to remove all discoloration. Polishing the parts is not
necessary; small scratches will not harm performance.
Abrasively clean discoloration where electrons from
filaments enter the source body.
Take care to avoid contaminating cleaned and dried parts.
Put on new, clean gloves before handling the parts. Do not
put the cleaned parts on a dirty surface. Place them only on
clean, lint-free cloths.

PARTS AND SUPPLIES

323

Ion Source

Chemical Ionization (CI) Ion Source

Tips & Tools
Visual appearance is not an
accurate guide to cleanliness of
the CI ion source. The CI ion source
can show little or no discoloration,
yet still need cleaning.

Because the CI ion source operates at much higher pressures than the EI ion source,
it will probably require more frequent cleaning than the EI ion source.
The source should be cleaned whenever there are performance anomalies that are associated
with a dirty ion source. Let analytical performance be your guide.
When cleaning the CI ion source, concentrate on the CI repeller, ion source body, and drawout
plate. Be sure to clean the 0.5 mm diameter holes in the ion source body and drawout plate.
Cleaning the ion source is very similar to cleaning the EI ion source. Use the same EI cleaning
procedure with the following exceptions:

• The CI ion source may not look dirty, but deposits left by chemical ionization are very difficult
to remove. Clean the CI ion source thoroughly.

• Use a round wooden toothpick to gently clean out the electron entrance hole in the source
body and the ion exit hole in the drawout plate.

• Do not use halogenated solvents. Use hexane for the final rinse.

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Ion Source

5975/5973 MSD Chemical Ionization Ion Source Parts (CI)
Item
1
2
3
5
4
6
7
8
9
10
11

Description
Source body
Drawout plate
Drawout cylinder
Ion focus lens
Lens insulator
Entrance lens
Repeller insulator
Repeller
High temperature filament
Cap screw, gold plated
Interface tip seal/spring

Part No.
G1999-20430
G1999-20446
G1999-20444
G1999-20443
G3170-20540
G3170-20126
G1999-20433
G1999-20432
G1099-80053
G1099-20021
G1999-60412

6
11

5
4
3

2
9

1
8
7

10

5975/5973 MSD Chemical Ionization (CI) ion source assembly

PARTS AND SUPPLIES

325

QuickSwap MS Interface Restrictors

QuickSwap MS Interface Restrictors
Agilent's QuickSwap Capillary Flow Technology module and pre-swaged fused silica tubing
restrictors can increase the productivity of your Agilent 5973N and 5975 Inert MSD Systems,
allowing you to change columns without venting the MSD. QuickSwap not included.
These restrictors are prefabricated for convenience and ease-of-use. For applications requiring
other restrictor sizes, Agilent offers a wide variety of deactivated fused silica tubing, SilTite ferrules
and swaging tools.

QuickSwap MS Interface Restrictors
Description
ID (mm)
QuickSwap restrictor
0.092
QuickSwap restrictor
0.100
QuickSwap restrictor
0.110
QuickSwap restrictor
0.120
QuickSwap restrictor variety pack, 2 each of the above ID restrictors

Unit
4/pk
4/pk
4/pk
4/pk

Part No.
G3185-60361
G3185-60362
G3185-60363
G3185-60364
G3185-60300

SilTite Metal Ferrules
Description
For use with 0.25 mm ID capillary columns
For use with 0.32 mm ID capillary columns
For use with 1/16 in. OD stainless steel tubing
Includes 2 column nuts
For use with 0.53 mm ID capillary columns

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Unit
10/pk
10/pk
10/pk

Part No.
5188-5361
5188-5362
5184-3571

10/pk

5188-5363

MSD Filaments

MSD Filaments
Like the filaments in an incandescent light bulb, the ion source filaments will eventually burn out.
Certain practices will reduce the chance of early failure.
Filament assembly (EI), G3170-60050

• When setting up data acquisition parameters, set the solvent delay so that the analyzer
will not turn on while the solvent peak is eluting
• When the software prompts 'Override solvent delay at the beginning of a run'
always select 'No'
• Higher emission current will reduce filament life
• If you control your MSD from the Edit Parameters screen, always select 'MS Off'
before changing any of the filament parameters

MSD Filaments
Description
Filament assembly (EI)
Filament assembly (CI)
Micro ion vacuum gauge
Triode gauge tube for measuring vacuum
Ion gauge controller
Ion gauge tube

5975 Series
G3170-60050
G1099-80053
G3170-80001

5975T Series

5973 Series
G3170-60050
G1099-80053

5972 Series
G3170-60050

5971 Series
05971-60140

0960-0897
G3880-80010
G3880-80011

Tips & Tools
It is very useful to switch from one filament to the other
every three months so that when a filament fails, you know
the other will fail soon. This will allow you to change both
filaments at the same time. Since the GC/MS system is
already vented, it's a good idea to replace other supplies
in the flowpath at the same time as the filaments.

PARTS AND SUPPLIES

327

MSD Electron Multipliers and Replacement Horn

MSD Electron Multipliers
and Replacement Horn
Electron multiplier replacement horn

The lifetime of an electron multiplier is directly related to the current that flows through it
and the extent of contamination or condensation that it experiences. Replace the electron
multiplier or replacement horn when voltage is over 2500 V. To maximize electron multiplier life:

• Maintain the best possible vacuum, especially in the analyzer manifold
• Use extreme caution and be conservative with venting, pumpdown, and all vacuum system
procedures to keep pump fluid background to a minimum
• After venting, allow four hours for pumpdown and thermal equilibration before scanning
• Actively look for background contamination and leaks and repair them immediately
• Don't tune excessively – PFTBA can result in higher background over an extended
period of time
• Replace the electron multiplier if vacuum is poor or voltage is over 2500 V

MSD Electron Multipliers and Replacement Horn
Description
Electron multiplier replacement horn
Use with electron multipliers with
"straight" horns
Triple axis detector assembly*
Triple axis electron multiplier
EM signal wire, low noise detector
High energy dynode
Electron multiplier

5975 Series
05971-80103

5973 Series
05971-80103

G3170-80100
G3170-80103
G3170-80008
G1099-80001

*Included on 5975 triple axis detector systems

Tips & Tools
The Agilent multipliers and horns listed are recommended for your
MSD. Other manufacturers' products may be incompatible with
Agilent instruments and can result in reduced sensitivity, lifetime,
and noise problems.

328

GC AND GC/MS

5972/5971 Series
05971-80103

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05971-80102

Vacuum Systems and Pumps

Vacuum Systems and Pumps
Diffusion Pump
It is not necessary to change the diffusion pump fluid more than once a year, unless you observe
symptoms that suggest a problem with the fluid. The MSD must be vented in order to check
the diffusion pump fluid (except for the 5975/5973). Therefore, the best time to check the fluid
is when the instrument is already vented for other maintenance.
Foreline Pump

Foreline Pump
The oil in the foreline or rough pump should be replaced on average once every six months,
but can vary depending upon applications. If a foreline trap is present, the molecular sieves
should also be replaced after an oil change.
Avoid contact with the pump oil. The residue from some samples may be toxic.
Dispense of used oil properly.

Pump Oils
Description
Foreline pump oil, Inland 45, 1 L
High vacuum grease, 25 g
Diffusion pump fluid, 18.5 mL
IDP Series tip seal kit for 5975T

Part No.
6040-0834
6040-0289
6040-0809*
IDP3TS

*2 required for 5975 and 5973 Series

PARTS AND SUPPLIES

329

7000A Triple Quadropole GC/MS Series

7000A Triple Quadropole GC/MS
Parts and Supplies
Engineered from the ground up for ease-of-use and routine high performance operation,
the 7000A Triple Quadrapole GC/MS delivers advanced high-speed GC/MS/MS quantitation
for ultra-trace analysis of even the most complex samples. Combined with the Agilent 7890 GC,
the result is an optimally robust GC/MS/MS system.

Gas Filters
Description
Chemical Ionization Gas Purifier
Big Universal Trap, 1/8 in. fittings, Helium (Ar/Me)
Big universal trap, 1/8 in. fittings, Nitrogen
Mounting clip

Part No.
G1999-80410
RMSH-2
RMSN-2
UMC-2

Foreline Pump Supplies
Description
Diffusion pump fluid, 18.5 mL
Foreline pump oil, P3, 0.5 L
Rough pump inlet flange
Oil return kit
Pump oil drip pan
Oil mist exhaust filter
Oil mist filter for RV5 pump

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Part No.
6040-0809
6040-0621
0905-1463
3162-1057
G1946-00034
G1099-80039
G6600-80043

7000A Triple Quadropole GC/MS Series

Maintenance Supplies

Low noise EM horn, G3170-80103

Description
Abrasive sheets
Alumina powder, abrasive, 1 kg
Cloths, lint-free
Lint-free industrial wipes, 100% cotton
Cotton swabs
Nylon gloves, lint-free, large
Nylon gloves, lint-free, small
High vacuum grease, 25 g
Electron multiplier replacement horn
Low noise EM horn
Filament assembly, high temperature (EI)
Filament assembly (CI)
Micro ion vacuum gauge
Replacement glass bulb for PFTBA and PFDTD test sample

Part No.
5061-5896
8660-0791
05980-60051
9310-4828
5080-5400
8650-0030
8650-0029
6040-0289
05971-80103
G3170-80103
G3170-60050
G1099-80053
G3170-80001
G3170-80002

Filament assembly (EI), G3170-60050

PARTS AND SUPPLIES

331

240-MS Ion Trap

240-MS Ion Trap Parts and Supplies
The Agilent 240-MS Ion Trap delivers unparalleled capabilities for both research and routine
applications. Advanced ionization, including positive and negative chemical ionization, improves
selectivity and limits of detection. Enhanced scanning techniques ensure compound confirmation.
The MS/MS and MSn reduce matrix influences and provide more detailed structural information.
The software comes with a full complement of productivity, reporting, and regulatory
compliance tools.

• Accurate identification and quantification of trace analytes
• Unsurpassed sensitivity (200 femtogram OFN full scan)
• Choice of internal or external ionization configurations
• Powerful MS/MS and CI options
• Low maintenance and high reliability
• Intuitive software for increased productivity
240-MS Ion Trap Parts and Supplies
Description
Manifold O-ring
Transfer line inner O-ring
Transfer line outer O-ring
Internal filaments (2 filaments on one disk)
Internal transfer line tip
External filament (single filament)
Electrode, end cap, Silchrom
Electrode set kit, Silchrom, DFC (inert) tested
Includes 2 end cap electrodes, 1 RF electrode, cleaning instructions
Electrode, RF, Silchrom
Spacer, RF, Silco-quartz
Electron multiplier
Transfer line assembly upgrade field kit
Contains a complete transfer line and side-mounted block for vacuum manifold
EPA volatile kit for EPA methods 524.2 & 8260B
ChromatoProbe microvials, 100/pk

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Part No.
393010924
393010920
393010918
392017401
393171201
393161001
393164493
9300003590
393167593
393053502
393175101
393101291
393082491
392567111

240-MS Ion Trap

240-MS Ion Trap Parts and Supplies
Description

Part No.

GC/MS Standards

Evaluation standard (Internal EI & CI) 2 pg/µL OFN, 5 pg/µL
Test standard for external EI (5 pg/µL OFN)
Benzophenone CI sensitivity standard 50 pg/mL
Test standard for external NCI (1 pg /µL DFB)
Tuning calibration compound PFTBA (FC-43)
GC/MS column test mix

393112601
393112702
392030500
393113001
392035300
392027300

Vacuum Supplies

Oil mist exhaust filter, DS42
Oil mist eliminator
Replacement cartridge for oil exhaust filter, 2/pk
Foreline (roughing) pump oil, 1 L
Premium foreline (roughing) pump oil, 1 L
IDP-3 dry scroll pump tip seal maintenance kit
IDP-3 dry scroll replacement module

393847701
2735000500
2710100200
8829951700
8829953800
2710100400
2710100500

PARTS AND SUPPLIES

333

220-MS

220-MS Parts and Supplies
The 220-MS is a high sensitivity, flexible gas chromatograph/mass spectrometer that delivers
outstanding qualitative and quantitative data in a range of applications. This simple and robust
system is easy to operate and maintain.

• Accurately identify and quantify trace analytes
• Take advantage of powerful CI and MS/MS upgrades for advanced applications
• Spend less time on maintenance and more time on analysis
220-MS Parts and Supplies
Description
Electron multiplier assembly
Exit end cap electrode, chrome
Exit end cap electrode, SilChrom
Filament end cap electrode, chrome
Filament end cap electrode, SilChrom
RF ring electrode, chrome
RF ring electrode, SilChrom
Complete set of SilChrom electrodes and Silco-quartz spacers
Spacer, RF, quartz
Spacer, RF, Silco-quartz
Filament disk assembly with wire connectors
Filament disk assembly
User must solder on 3 wire connectors
Thermocouple vacuum gauge
Mass spectrometer expendable supplies kit for 2x0MS
Includes PFTBA calibration compound, cal-gas glass chamber, capillary injector nut,
O-rings, cotton tipped applicators, end cap insulator, vacuum pump oil

Part No.
393031501
393050292
393050293
393050392
393050393
393050492
393050493
393001991
393053501
393053502
393060191
392043700
2722990700
393011391

GC/MS Standards

Hexachlorobenzene EI sensitivity standard 100 pg/mL
Benzophenone CI sensitivity standard 50 pg/mL
Tuning calibration compound PFTBA (FC-43)
Hexachlorobenzene EI sensitivity standard 2 pg/mL
GC/MS column test mix

334

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l 01357 522 961

392027500
392030500
392035300
392047100
392027300

GC/MS Standards

GC/MS Standards
GC/MS Analyzer Kit Standards
MS standards

Description
GC/MS semivolatiles analyzer checkout mixture
GC/MS pesticide analyzer internal standard
Pesticide analyzer checkout solution
Pesticide checkout standard, 100 µg/L, 3 x 1 mL
GC/MS toxicology checkout mixture
Residual solvent revised method 467, class C
Residual solvent revised method 467, class 1
Butanetriol internal standard #1 for biodiesel
Tricaprin internal standard #2 for biodiesel

Part No.
5190-0473
5190-0472
5190-0468
5190-0494
5190-0471
5190-0493
5190-0490
5982-0024
5982-0025

PARTS AND SUPPLIES

335

GC/MS Standards

MS Test and Performance Samples
Description

Part No.

5975
Series

5973
Series

5972
Series

5971
Series

]
]
]

]
]
]

]

]

]

]

GCD

7000
Series

Tuning Samples

EI Tune
CI Tune

PFTBA sample, certified, 10 g, 5.32 mL 8500-0656
PFTBA MS Sample Kit, 0.942 g, 0.5 mL 05971-60571
PFDTD calibrant
8500-8510

]
]

]
]
]

]

]

Performance Verification Samples

EI

OFN, 1 pg/µL
Hexachlorobenzene 10 pg/µL,
1 ng/µL
Methyl stearate (in methanol);
1 ng/µL, 2 ea
Sample A, 10 ng/µL
Negative Mode CI OFN, 100 fg/µL
Positive Mode CI Benzophenone, 100 pg/µL

5188-5348
8500-5808

]
]

05990-60075
05970-60045
5188-5347
8500-5440

]
]

]

]

]

5188-5357
8500-5995
8500-5851
05970-60045

]
]
]
]

]
]
]

]
]
]

]
]
]

]

Checkout Samples

HighMass
PHFT, 100 pg/µL
Semi-Volatile
GC/MS tuning standard, DFTPP
Volatile
p-Bromofluorobenzene (BFB), 25 µg/mL
Evaluation sample Solution of dodecane, biphenyl,
p-cholorodiphenyl, and Methyl
palmitate in isooctane.
Six 1.0 mL ampoules: 4 at 10 ng/µL,
1 at 100 ng/µL, 1 at 100 pg/µL.

Tips & Tools
Each GC/MS has a specific test and performance sample. Refer to the chart above for the exact
sample. All volumes are approximately 0.5-1 mL unless otherwise specified.

336

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l 01357 522 961

]
]

GC Parts and Supplies for Varian Instruments

GC Parts and Supplies
for Varian Instruments
Agilent now provides replacement parts and supplies for GC instruments formerly
manufactured by Varian. The following section includes ordering information for GC injectors,
detectors and GC/MS systems.

Injector Replacement Parts and Supplies
1177 Split/Splitless Injector
1
2
3
4

6
5
7
8

9

10
11

Item Description
1
Injector nut
Injector nut wrench
2
Knob
3
Automatic start switch
4
Septum, 9 mm
BTO
Long Life
Advanced Green
Septum pick
5
Septum purge head
EFC21 (stainless steel)
EFC21 (UltiMetal)
EFC25 or Manual Pneumatics
6
Purge head screw
7
Graphite liner O-ring, splitless, 6.5 mm
Viton liner O-ring, 6.3 mm
8
Glass liner
9
Injector body
Stainless steel
UltiMetal
Manual
10
For replacement ferrules, see Agilent's new line of CrossLab Supplies
11
Bottom nut

Part No.
392597501
390842300
392597101
390820601
8010-0217
8010-0233
8010-0201
7200008400
392597301
392597303
392597302
391866308
8004-0202
8004-0201
8004-0165
392599401
392599411
392599501
8004-0311

PARTS AND SUPPLIES

337

GC Parts and Supplies for Varian Instruments

1079 Large Volume Injector (LVI)
1a

1b

2

3
4
5

6

7

Item Description
1a
Injector nut
1b
Injector nut
Injector nut wrench
2
Automatic start switch
3
Septum, 11.5 mm
BTO
Long Life
Advanced Green
Septum pick
4
Septum support
5
Insert ferrule
6
Glass liner
7
Injector body, EFC type
Stainless steel
UltiMetal
8
For replacement ferrules, see Agilent's new line of CrossLab Supplies
9
Bottom nut

8
9

To learn more about Agilent CrossLab and to request your
copy of the Agilent CrossLab product catalog, visit
www.agilent.com/chem/CrossLab

338

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l 01357 522 961

Part No.
394966601
394966601
390842300
390820601
8010-0225
8010-0241
8010-0209
7200008400
391867600
8004-0204
8004-0164
392544001
392544011
8004-0311

GC Parts and Supplies for Varian Instruments

1093 Cool On-Column (COC) Injector

1
2

3
4

5

6

7

Item Description
1
Injector nut
Injector nut wrench
2
Automatic start switch
3
Septum, 11.5 mm
BTO
Long Life
Advanced Green
Septum pick
4
Septum support
5
Glass liner
Default
High performance
On-column
6
Screw
7
Graphite/Vespel ferrule
Graphite ferrule
8
Bottom nut
Brass
Stainless steel

Part No.
394966601
390842300
390820601
8010-0225
8010-0241
8010-0209
7200008400
391821100
8004-0162
8004-0167
8004-0166
391866306
8004-0217
8010-0305
8004-0311
8004-0312

8

PARTS AND SUPPLIES

339

GC Parts and Supplies for Varian Instruments

1a

1b
2

3

4

5

1061 Packed/530 µm Capillary Column Injector
Item Description
1a
Injector nut
1b
Injector nut
Injector nut wrench
2
Septum, 9.5 mm
BTO
Long Life
Advanced Green
Septum pick
3
Automatic start switch
4
Glass liner
5
Injector body, EFC23
6
Graphite/Vespel ferrule
Graphite ferrule
7
For replacement ferrules, see Agilent's new line of CrossLab Supplies
8
Bottom nut

6

7
8

340

GC AND GC/MS

www.agilent.com/chem/GC
www.crawfordscientific.com
l 01357 522 961

Part No.
390812700
392595501
390842300
8010-0219
8010-0235
8010-0203
7200008400
390820601
8004-0168
392548301
8004-0217
8010-0305
8004-0311

GC Parts and Supplies for Varian Instruments

1a

1b

3

2

4

1041 Packed/Wide Bore On-Column (PWOC) Injector
Item Description
1a
Injector nut
1b
Injector nut
Injector nut wrench
2
Septum, 9.5 mm
BTO
Long Life
Advanced Green
Septum pick
3
Automatic start switch
4
Injector body, EFC type
5
Graphite/Vespel ferrule
Graphite ferrule
6
Injector insert, stainless steel
7
For replacement ferrules, see Agilent's new line of CrossLab Supplies
8
Bottom nut

Part No.
390812700
392595501
390842300
8010-0219
8010-0235
8010-0203
7200008400
390820601
392548201
8004-0217
8010-0305
392543101
8004-0311

5
6

8

7

PARTS AND SUPPLIES

341

GC Parts and Supplies for Varian Instruments

Detector Replacement Parts and Supplies
Thermal Conductivity Detector (TCD)
Description
Adapter TCD/DEFC capillary make-up
Adapter TCD/DEFC reference gas kit
Adapter TCD capillary make-up, MPC, 3800
TCD DEFC 14 (Non-H2), 2 channels

Part No.
392585291
392585292
392560591
392561290

Flame Ionization Detector (FID)
Description
Tube collector
Lower FID insulator 17311
FID flame tip jet, 0.010 in.
FID flame tip jet with nut, 0.020 in.
Crunch washer, 25/pk

Tips & Tools
To learn more about Agilent's complete portfolio of services,
please visit www.agilent.com/chem/services

342

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Part No.
394958700
2100003200
200187500
200193800
1500334701

GC Parts and Supplies for Varian Instruments

Pulsed Flame Photometric Detector (PFPD)
Description
Photomultiplier tube (PFPD) #R647-08
O-Ring, silicone, 0.53 in. ID, PFPD
PFPD light pipe
Sapphire window assembly
Sapphire window washer
Wrench, PFPD combustor support
Seal, combustor support
Combustor holder (2 mm)
Combustor Sulfur (2 mm), cleaned
Holder, combustor, 3 mm, cleaned
Combustor Phosphorus, 3 mm, cleaned

Part No.
392517100
2740292400
392515500
392514500
392514300
392519200
392513800
392517800
392517600
392517901
392517700

PFPD Filter Assemblies
Description
Arsenic (As)
Manganese (Mn)
Nitrogen (N)
Sulfur and Phosphorus (S and P)
Phosphorus (P)
Sulfur (S)
Tin (Sn)

Part No.
392515105
392544391
392511901
392515104
392515102
392515101
392515103

PARTS AND SUPPLIES

343

GC Parts and Supplies for Varian Instruments

PFPD Nitrogen Mode Maintenance
Description
Photomultiplier tube, Nitrogen R-5070A
O-Ring, 0.987 in. ID
PFPD filter assembly, Nitrogen
PFPD light pipe
Sapphire window assembly
Sapphire window washer

Part No.
392512800
2740236100
392511901
392515500
392514500
392514300

Thermionic Specific Detector (TSD)
Description
TSD bead probe, unconditioned and untested
TSD bead probe, conditioned and tested
Upper TSD insulator #17310 TSD
O-Ring, 30/pk
TSD collector assembly
Lower FID insulator #17311
Crunch washer, 25/pk
FID flame tip jet with nut, 0.020 in.
Flow tube assembly

344

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l 01357 522 961

Part No.
390607400
390607401
2100003100
2740928202
390607900
2100003200
1500334701
200193800
200187600

GC Parts and Supplies for Varian Instruments

GC/MS System Replacement Parts and Supplies
210/220/225 GC/MS Systems
Description
Electron multiplier assembly
Exit end cap electrode, chrome
Exit end cap electrode, SilChrom
Filament end cap electrode, chrome
Filament end cap electrode, SilChrom
RF ring electrode, chrome
RF ring electrode, SilChrom
Complete set of SilChrom electrodes and Silco-quartz spacers
Spacer, RF, quartz
Spacer, RF, Silco-quartz
Filament disk assembly with wire connectors
Filament disk assembly
User must solder on 3 wire connectors
Thermocouple vacuum gauge
Mass spectrometer expendable supplies kit for 2x0MS
Includes PFTBA calibration compound, cal-gas glass chamber, capillary injector nut,
O-rings, cotton tipped applicators, end cap insulator, vacuum pump oil

Part No.
393031501
393050292
393050293
393050392
393050393
393050492
393050493
393001991
393053501
393053502
393060191
392043700
2722990700
393011391

GC/MS Standards

Hexachlorobenzene EI sensitivity standard 100 pg/mL
Benzophenone CI sensitivity standard 50 pg/mL
Tuning calibration compound PFTBA (FC-43)
Hexachlorobenzene EI sensitivity standard 2 pg/mL
GC/MS column test mix

392027500
392030500
392035300
392047100
392027300

PARTS AND SUPPLIES

345

GC Parts and Supplies for Varian Instruments

240 GC/MS and 4000 GC/MS Systems
Description
Manifold O-ring
Transfer line inner O-ring
Transfer line outer O-ring
Internal filaments (2 filaments on one disk)
Internal transfer line tip
External filament (single filament)
Electrode, end cap, SilChrom
Electrode set kit, SilChrom, DFC (inert) tested
Includes 2 end cap electrodes, 1 RF electrode, cleaning instructions
Electrode, RF, SilChrom
Spacer, RF, Silco-quartz
Electron multiplier
Transfer line assembly upgrade field kit
Contains a complete transfer line and side-mounted block for vacuum manifold
EPA volatile kit for EPA methods 524.2 & 8260B
ChromatoProbe microvials, 100/pk

Part No.
393010924
393010920
393010918
392017401
393171201
393161001
393164493
9300003590
393167593
393053502
393175101
393101291
393082491
392567111

GC/MS Standards

Evaluation standard (Internal EI & CI) 2 pg/µL OFN, 5 pg/µL
Test standard for external EI (5 pg/µL OFN)
Benzophenone CI sensitivity standard 50 pg/mL
Test standard for external NCI (1 pg /µL DFB)
Tuning calibration compound PFTBA (FC-43)
GC/MS column test mix

393112601
393112702
392030500
393113001
392035300
392027300

Vacuum Supplies

Oil mist exhaust filter, DS42
Oil mist eliminator
Replacement cartridge for oil exhaust filter, 2/pk
Foreline (roughing) pump oil, 1 L
Premium foreline (roughing) pump oil, 1 L
IDP-3 dry scroll pump tip seal maintenance kit
IDP-3 dry scroll replacement module

346

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l 01357 522 961

393847701
2735000500
2710100200
8829951700
8829953800
2710100400
2710100500

GC Parts and Supplies for Varian Instruments

Saturn 2000 Series MS Systems
Description
Mass spectrometer expendable supplies kit for 2x0MS
Includes PFTBA calibration compound, cal-gas glass chamber, capillary injector nut,
O-rings, cotton tipped applicators, end cap insulator, vacuum pump oil
Electron multiplier assembly
Filament disk assembly with wire connectors
Filament disk assembly
User must solder on 3 wire connectors
Exit end cap electrode, SilChrom
Filament end cap electrode, SilChrom
RF ring electrode, SilChrom
Exit end cap electrode, chrome
Filament end cap electrode, chrome
RF ring electrode, chrome
Spacer, RF, quartz
Spacer, RF, Silco-quartz
Complete set of SilChrom electrodes and Silco-quartz spacers

Part No.
393011391

393031501
393060191
392043700
393050293
393050393
393050493
393050292
393050392
393050492
393053501
393053502
393001991

GC/MS Standards

Tuning calibration compound PFTBA (FC-43)
Benzophenone CI sensitivity standard 50 pg/mL
Hexachlorobenzene EI sensitivity standard 2 pg/mL
Hexachlorobenzene EI sensitivity standard 100 pg/mL
GC/MS column test mix

392035300
392030500
392047100
392027500
392027300

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

PARTS AND SUPPLIES

347

Agilent J&W GC and GC/MS Columns

AGILENT J&W GC
AND GC/MS COLUMNS
A legacy of confidence:
The continuing story
of Agilent J&W GC Columns
In 2000, Agilent Technologies, the inventor of low-bleed HP-5ms columns, merged with J&W
Scientific, the creator of DB-5ms – the first GC stationary phase to use arylene technology for
lower column bleed.
Thanks to this partnership, you can find both the renowned HP and DB column families under the
Agilent J&W name. And they’re all brought to you by Agilent Technologies – a company with
over 40 years of gas chromatography experience.

In 2010, a new chapter began
with the addition of Varian, Inc.
This key acquisition enabled Agilent to expand its GC column portfolio to include three highly
respected column families:

• FactorFour GC columns employ advanced proprietary manufacturing techniques to ensure
low background and high signal-to-noise values for your routine or trace analysis.

• Select GC columns offer guaranteed performance for specific methods and applications.
• PoraBOND GC columns are highly retentive Porous Layer Open Tubular (PLOT) columns.
Because they do not shed any particles, they can improve the quality of your data for
volatile compounds.

348

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Agilent J&W GC and GC/MS Columns

The Agilent J&W column difference
Low bleed and high inertness
for sensitivity and performance
Column bleed can decrease data integrity, reduce uptime, and shorten column life, while column
activity contributes to severe peak tailing, compound loss, and degradation of active compounds,
all of which raise detection limits and reduce method linearity.
Agilent J&W columns have the widest range of GC/MS and Ultra Inert stationary phases proven
to deliver consistent column inertness, exceptionally low column bleed, and high upper
temperature limits to ensure accurate peak identification and quantification, improved sensitivity,
and extended linearity.

Better precision for better results
and greater productivity
Every Agilent J&W GC column adheres to tight retention factor (k) specifications, promoting
consistent retention and separation. They also feature narrow retention indexes and a high
number of theoretical plates per meter, both of which provide narrow peaks, improve the
resolution of closely eluting peaks, and simplify data interpretation.

The industry's tightest
quality control specifications
Agilent's stringent testing ensures reliable qualitative and quantitative results – even for your
most challenging compounds. For example, we measure peak height ratios for acids and bases
to achieve top performance for the widest range of analytes.
In 2008, Agilent also ushered in a new era of column inertness QC testing with the industry's
most rigorous test probe mixture.

AGILENT J&W GC AND GC/MS COLUMNS

349

Agilent J&W GC and GC/MS Columns

Tighter GC Column Performance Criteria
Feature
Narrow Retention Index
and Retention Factor
Window

Advantage

Benefit

� Highest level of column-to-column reproducibility

� Confidence in analytical results

� Minimal method adjustment when changing columns

� Improved sample throughput, reduced downtime

� Specify J&W chemistry for intra-company
methodologies
Increased Plates per Meter � Highest level of "resolving power"
� Improved sample throughput
Lowest Bleed
� Increased analytical sensitivity for all detectors

Highest Degree of
Inertness

350

GC AND GC/MS

� Confidence in method transfer and intra-company
results
� Accurate quantification
� Potentially shorter run times
� Improved detection limits
� Reliable compound identification

� Fast baseline stabilization
� Faster column exchange

� Minimized conditioning time
� Increased sample throughput

� Excellent thermal stability

� Increased column lifetime, reduced downtime
� Increased sample throughput

� Better peak shape for active compounds
� Minimum compound adsorption

� Improved detection limit, more accurate quantification,
and more instrument uptime
� More accurate quantification for trace samples and
unknown sample screening

www.agilent.com/chem/gccolumns l 01357 522 961
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Agilent J&W GC and GC/MS Columns

Agilent J&W Ultra Inert GC Capillary Columns
The only GC columns that deliver on
the promises of consistent column inertness
and exceptionally low column bleed
Agilent J&W Ultra Inert GC columns give you outstanding sensitivity and peak shape, allowing
you to confidently perform trace-level analysis of acids, bases, and other active compounds.
Like all Agilent columns, Ultra Inert GC columns undergo tight QC testing procedures. However,
Ultra Inert columns must also pass through a more difficult set of test parameters, including:

• A demanding test probe mixture that contains compounds with low molecular weights, low
boiling points, and no steric shielding of active functional groups, which prevents masking
effects and reveals true column quality.
• Testing at lower isothermal temperatures (65°C vs. 120°C for GC/MS columns). Lowertemperature testing decreases the kinetic energy of probes in the mobile phase, preventing
molecules from sweeping past active sites on the column. This allows a true evaluation of
column surface activity and ensures consistent column inertness.
Together, these conditions enhance the opportunity for solute/column interactions, and expose
column deficiencies that traditional GC/MS testing might not detect.

AGILENT J&W GC AND GC/MS COLUMNS

351

Agilent J&W GC and GC/MS Columns

Agilent J&W High Efficiency GC Capillary Columns
High efficiency, high throughput,
and high resolution without the high costs
This leading-edge column technology is ideal for applications that require faster run times, such
as high-throughput screening, fast process monitoring, and fast method development. In fact,
Agilent High Efficiency columns can reduce your sample run time by 50% or more without
compromising resolution.
Unlike other manufacturers' 0.1 mm ID columns, Agilent High Efficiency Capillary GC columns are
compatible with all standard-pressure capillary GC and GC/MS instruments – without expensive
high-pressure modifications. They also give you:

• The flexibility to choose between helium and hydrogen carrier gases. You can stay with

a helium carrier if you wish to simplify method development, or switch to a hydrogen carrier
to further reduce your analysis time.
• The ability to separate samples using less carrier gas, which can lead to longer intervals
between cylinder changes, increased uptime, and a lower cost per sample.
In addition, these flexible columns easily adapt to a wide variety of environmental, petrochemical,
flavor/fragrance, clinical toxicology, and pharmaceutical sample matrices.

352

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Agilent J&W GC and GC/MS Columns

Agilent J&W FactorFour GC Columns
Low-bleed performance for all areas
of GC and GC/MS analysis
With Agilent J&W FactorFour columns, you get the throughput you need to analyze more
compounds in less time – and the analytical accuracy you need to get the right results every
time. They are manufactured from detailed specifications and high-quality materials to ensure
the reproducibility, low bleed, and high inertness that today’s applications demand.
FactorFour columns are available in a variety of general and application-specific phases –
including VF-WAXms, the only GC/MS-compatible Wax column – to meet your lab’s
every requirement.

Double your sample throughput and reduce your cost
per analysis with FactorFour 0.15 mm ID columns
In addition to standard dimensions, FactorFour columns are available in an extended range
of 0.15 mm ID choices specifically designed to reduce run times and increase throughput.

AGILENT J&W GC AND GC/MS COLUMNS

353

Agilent J&W GC and GC/MS Columns

Agilent J&W LTM Column Modules
Shorten analytical cycle times and boost
your high speed gas chromatography capabilities
Agilent J&W LTM column modules combine a high quality fused silica capillary column with
heating and temperature sensing components for a low thermal mass column assembly. The LTM
column module contains a patented design which heats and cools the column very efficiently for
significantly shorter analytical cycle times compared to conventional air-bath GC oven techniques,
while simultaneously using less power.
Agilent offers LTM technology for our popular 7890 and 6890 Series GC systems, as well as the
new 5875T GC/MS.
All LTM column modules are packaged with:

• Two 1 m guard columns (one each for the inlet and detector) made from deactivated fused
silica tubing of the same ID as the analytical column
• Five non-reusable ferrules that fit the dimensions of the analytical and guard columns

Tips & Tools
Agilent LTM column module technology is compatible with metal
capillary columns. However, LTM modules are generally not
recommended for fast GC applications because of their poor
cooling performance compared to fused silica capillaries.

354

GC AND GC/MS

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Agilent J&W GC and GC/MS Columns

Agilent J&W LTM Column Modules
for 7890 and 6890 GC Systems
This groundbreaking column technology is designed specifically for Agilent 7890A and 6890
series gas chromatographs, and delivers:

• The capacity to run up to four column modules simultaneously – with four different
temperature programs – to maximize your productivity

• Rapid temperature programming rates of up to 1800°C/min for higher analysis speeds
• Faster cooling times – as low as one minute or less – to decrease idling and downtime
• Shorter analytical cycle times than conventional air-bath GC oven techniques
• Excellent retention time repeatability and performance – comparable to conventional GC
• Strong synergy with Agilent Capillary Flow Technology, which can enhance your ability to
perform multi-dimensional and comprehensive GC

• The ability to use the same 6890/7890 GC injectors and detectors with little change to your
existing methods

Most Agilent J&W Capillary GC columns – including Wall Coated Open Tubular (WCOT)
and Porous Layer Open Tubular (PLOT) columns – can be used for LTM column modules.
Module assemblies are available in two sizes that deliver equal chromatographic quality:

• 5 inch diameter (standard): features faster cooling speeds, and is recommended for most

applications. The 5 inch format allows two column modules to be installed per Agilent LTM
system, and can be used with any capillary GC column up to 30 m long.
• 3 inch diameter (small): enables multidimensional GC applications by allowing up to four
column modules to be installed per Agilent LTM system. The 3 inch format is limited to
capillary GC columns with a 0.32 mm or smaller ID, and is not recommended for fragile
capillary columns or PLOT columns.
Module sizes can also be mixed; for example, you can use one 5 inch module with either one or
two 3 inch modules.

Tips & Tools
LTM column modules should never be programmed beyond the GC
column temperature limits recommended by Agilent. For very fast
ramping applications (e.g. 600°C/min), limiting the maximum
temperatures to 10-20°C below the GC column temperature limits
can increase the lifetime of the column modules.

AGILENT J&W GC AND GC/MS COLUMNS

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Agilent J&W GC and GC/MS Columns

NEW!

Agilent J&W LTM Column Modules for 5975T
Transportable GC/MS Systems
This groundbreaking column technology is designed specifically for Agilent 5975T GC/MS
systems. These modules include integrated 3 inch LTM capillary column toroid assembly with
heated transfer lines, cooling fan assembly and sheet metal enclosure. Replacement column
toroid assemblies are also available. Benefits of the LTM column modules include:

• Rapid temperature programming rates of up to 1200°C/min
• Faster heating and cooling times – as low as one minute or less – for more rapid analytical
cycle times than standard air-bath GC oven techniques

• Excellent retention time repeatability and performance comparable to conventional GC
• Less power consumption for longer in-field operation
• Integrated module design to facilitate easy column module change in the field
The entire assembly is leak tested and ready for installation into your Agilent 5975T instrument.
LTM column modules for the 5975T include:

• 2 heated transfer lines
• Transferline base assembly
• 3 inch LTM column toroid assembly
• 2 ultimate unions
• Cooling fan assembly
• Sheet metal enclosure
For more information, visit www.agilent.com/chem/LTMcol

356

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Agilent J&W GC and GC/MS Columns

Agilent J&W LTM Column Modules for 5975T Transportable GC/MS Systems
Phase
DB-5ms Ultra Inert

ID (mm)
0.18
0.25

HP-5ms Ultra Inert

0.18
0.25

DB-1
DB-1ms

0.25
0.18
0.25

DB-1ht

0.25

HP-1ms

0.18
0.25

DB-5ms

0.18
0.25

DB-5ht

0.25

HP-5ms
DB-35ms

0.25
0.18
0.25

DB-17ms

0.18
0.25

DB-225ms

0.25

DB-1701
DB-WAX

0.25
0.25

HP-INNOWax

0.18
0.25
0.25

DB-FFAP
DB-608
DB-VRX
DB-624
HP-VOC

0.18
0.18
0.25
0.18
0.25
0.20

Length (m)
20
15
30
20
15
30
30
20
15
30
15
30
20
30
15
20
15
30
15
30
30
20
15
30
20
15
30
15
30
30
15
30
20
30
15
30
20
20
30
20
30
30

Film (µm)
0.18
0.25
0.25
0.18
0.25
0.25
0.25
0.18
0.25
0.25
0.10
0.10
0.18
0.10
0.25
0.18
0.25
0.25
0.10
0.10
0.25
0.18
0.25
0.25
0.18
0.25
0.25
0.25
0.25
0.25
0.50
0.50
0.18
0.25
0.25
0.25
0.18
1.00
1.40
1.00
1.40
1.12

Toroid
Assembly
221-5522UILTM
222-5512UILTM
222-5532UILTM
29091S-577UILTM
29091S-431UILTM
29091S-433UILTM
222-1032LTM
221-0122LTM
222-0112LTM
222-0132LTM
222-1111LTM
222-1131LTM
29091S-677LTM
29091S-833LTM
29091S-931LTM
221-5522LTM
222-5512LTM
222-5532LTM
222-5731LTM
222-5711LTM
29091S-433LTM
221-3822LTM
222-3812LTM
222-3832LTM
221-4722LTM
222-4712LTM
222-4732LTM
222-2912LTM
222-2932LTM
222-0732LTM
222-7013LTM
222-7033LTM
29091N-577LTM
29091N-133LTM
222-3212LTM
222-3232LTM
221-6822LTM
221-1524LTM
222-1534LTM
221-1324LTM
222-1334LTM
29091R-303LTM

Column
Module
G3900-63014
G3900-63031
G3900-63005
G3900-63039
G3900-63038
G3900-63001
G3900-63002
G3900-63009
G3900-63016
G3900-63017
G3900-63018
G3900-63019
G3900-63040
G3900-63041
G3900-63042
G3900-63013
G3900-63030
G3900-63004
G3900-63033
G3900-63032
G3900-63007
G3900-63011
G3900-63026
G3900-63027
G3900-63012
G3900-63028
G3900-63029
G3900-63022
G3900-63023
G3900-63003
G3900-63034
G3900-63035
G3900-63036
G3900-63008
G3900-63024
G3900-63025
G3900-63015
G3900-63006
G3900-63021
G3900-63010
G3900-63020
G3900-63037

AGILENT J&W GC AND GC/MS COLUMNS

357

Agilent J&W GC and GC/MS Columns

Choosing a Capillary GC Column
The first step should always be to refer to what has already been done. Our chemists have put
together a variety of resources to help you find the right column for your analysis.

• Chromatograms – Find some of the more common chromatograms with column

recommendations and method parameters for your reference in this catalog. For a more
extensive chromatogram library and a compound search function, go to
www.agilent.com/chem, then click Library.

• Method Guides – We've evaluated the most common Environmental/EPA methods, ASTM
methods, USP methods and general compound analyses and compiled simple guides which
specify the best column recommendation for these methods.

• Retention Data Lists – We've analyzed hundreds of compounds on several different
phases to help you determine which column will be the best choice for your list of
compounds.

• Column Selection Guide – Our Agilent J&W GC Column Selection Guide gives you helpful
hints for choosing a stationary phase, selecting the right column dimensions, developing
temperature programs and determining the right inlet and detector for the application.
To order this guide, use publication number 5989-6159EN.

Our Technical Support Chemists have more than 100 years of combined experience running
samples and developing methods. We are the gas chromatography column experts and we
are at your disposal.
Send in questions via the Technical Support form on our website www.agilent.com/chem,
via email at gc-column-support@agilent.com, or contact your local Agilent office or Authorized
Agilent Distributor.

358

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Ultra Inert Capillary GC Columns

Tips & Tools
Agilent Ultra Inert Liners are the
perfect companion to Agilent J&W
Ultra Inert GC Columns, providing
reproducible inertness liner after liner,
maintained through a sequence of
samples, and for a range of analytes.
Turn to page 261.

Agilent J&W Ultra Inert
Capillary GC Columns
• Individually tested with a unique, demanding test probe mixture
• Consistent column inertness performance
• Exceptionally low column bleed
• Great peak shapes for challenging active analytes
• Excellent signal-to-noise ratios
• Minimum compound adsorption or degradation
• Support of 0.18 mm ID column configuration for higher sample throughput
Ultra Inert Chromatograms
Environmental
Trace Level Polycyclic Aromatic Hydrocarbon (PAH) Analyses
US EPA Method 8270 Short Mix
US EPA Method 551.1

Page 589
Page 589
Page 590

Life Sciences
Benzodiazepines I

Page 671

Food, Flavors and Fragrances
Lavender Oil Characterization

Page 622

DB-1ms Ultra Inert
ID (mm)
0.18
0.25

0.32

Length (m)
20
15
30
60
15
30

Film (µm)
0.18
0.25
0.25
0.25
0.25
0.25

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

7 in. Cage
121-0122UI
122-0112UI
122-0132UI
122-0162UI
123-0112UI
123-0132UI

5 in. Cage
122-0112UIE
122-0132UIE

AGILENT J&W GC AND GC/MS COLUMNS

359

Ultra Inert Capillary GC Columns

HP-1ms Ultra Inert
ID (mm)
0.18
0.25

Length (m)
20
15
30

0.32

15
25
30

Film (µm)
0.18
0.25
0.25
0.50
1.00
0.25
0.52
0.25
1.00

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

7 in. Cage
19091S-677UI
19091S-931UI
19091S-933UI
19091S-633UI
19091S-733UI
19091S-911UI
19091S-612UI
19091S-913UI
19091S-713UI

5 in. Cage
19091S-677UIE
19091S-931UIE
19091S-933UIE

Film (µm)
0.18
0.25
0.25
0.25
0.25

Temp Limits (°C)
50 to 340/360
50 to 340/360
50 to 340/360
50 to 340/360
50 to 340/360

19091S-733UIE

19091S-913UIE

DB-35ms Ultra Inert
ID (mm)
0.18
0.25
0.32

360

GC AND GC/MS

Length (m)
20
15
30
15
30

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7 in. Cage
121-3822UI
122-3812UI
122-3832UI
123-3812UI
123-3832UI

Ultra Inert Capillary GC Columns

DB-5ms Ultra Inert
ID (mm)
0.18

Length (m)
20

0.25

40
15
25
30

50
60
0.32

30

60

Film (µm)
0.18
0.36
0.18
0.25
1.00
0.25
0.25
0.50
1.00
0.25
0.25
1.00
0.25
0.50
1.00
1.00

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

7 in. Cage
121-5522UI
121-5523UI
121-5542UI
122-5512UI
122-5513UI
122-5522UI
122-5532UI
122-5536UI
122-5533UI
122-5552UI
122-5562UI
122-5563UI
123-5532UI
123-5536UI
123-5533UI
123-5563UI

5 in. Cage

122-5532UIE
122-5533UIE

123-5532UIE

7890/6890
LTM Module
121-5522UILTM
121-5523UILTM

5975T
LTM Toroid
221-5522UILTM

122-5512UILTM
122-5513UILTM
122-5522UILTM
122-5532UILTM
122-5536UILTM
122-5533UILTM

222-5512UILTM

222-5532UILTM

123-5532UILTM
123-5536UILTM
123-5533UILTM

HP-5ms Ultra Inert
ID (mm)

Length (m) Film (µm)

Temp Limits (°C)

7 in. Cage

-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

19091S-577UI
19091S-431UI
19091S-433UI
19091S-133UI
19091S-233UI
19091S-436UI
19091S-413UI
19091S-213UI

5 in. Cage

7890/6890
LTM Module

5975T
LTM Toroid

19091S-577UILTM
19091S-431UILTM
19091S-433UILTM
19091S-133UILTM
19091S-233UILTM

29091S-577UILTM
29091S-431UILTM
29091S-433UILTM

HP-5ms Ultra Inert

0.18
0.25

0.32

20
15
30

60
30

0.18
0.25
0.25
0.50
1.00
0.25
0.25
1.00

19091S-433UIE
19091S-233UIE

19091S-413UILTM
19091S-213UILTM

AGILENT J&W GC AND GC/MS COLUMNS

361

Guard Columns

Guard Columns

Tips & Tools
Column contamination from sample
matrix components is the number one
cause of column failure. Use Agilent
DuraGuard GC columns with built-in
guard if you do not want to use
column connectors.

• Columns with "built-in" guard columns, no press-fit connectors
• Minimize front-end contamination and increase column lifetime
• Aid in focusing sample onto the front of the column for better peak shape
• Minimize MSD contamination originating from the column (when used in transfer line)
Guard columns (or retention gaps) are often added to the front of the analytical column to
protect against contamination, or to act as a band-focusing device for liquid samples introduced
by on-column and splitless injection techniques.
When resolution or response in a chromatogram diminishes, remove a coil from the guard
column so that peak shapes will improve. By removing a coil, the column length is shortened
and peaks will elute somewhat faster. For best results, check the integration time windows
of your data system.

DuraGuard
DuraGuard columns of different phases and dimensions are available through Agilent
Technologies' custom column shop. Any DB polysiloxane or GC/MS phase can be made as a
DuraGuard column with 0.18 mm ID or larger fused silica tubing. Ask for a custom column quote
using part number 100-2000. Specify the phase, ID, length, and film thickness of analytical
column, and desired length of DuraGuard.

DuraGuard
Phase
DB-1
DB-XLB
DB-5ms

DB-5.625
DB-1701
DB-624

362

GC AND GC/MS

ID (mm)
0.25
0.25
0.25

Length (m)
30
30
30

0.32
0.53
0.18
0.25
0.53
0.53

60
30
30
20
30
30
30

Film (µm)
0.25
0.25
0.25
0.50
1.00
0.25
1.00
0.50
0.36
0.25
1.00
3.00

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Guard Length (m)
10
10
10
10
10
10
10
10
5
5
10
5

Part No.
122-1032G
122-1232G
122-5532G
122-5536G
122-5533G
122-5562G
123-5533G
125-5537G
121-5622G5
122-5631G5
125-0732G
125-1334G5

Guard Columns

EZ-Guard
EZ-Guard columns combine a FactorFour column with a built-in guard column. The first five or
ten meter section of the EZ-Guard column (guard length depends on the column you select) is
not coated with stationary phase, but has been deactivated. The lack of a column connection
between the guard and analytical section results in a 100% leak-free column.
Every EZ-Guard column features a unique uncoated and deactivated outlet section,
approximately 100 cm long, which acts as an integrated transfer line. This provides
a shorter stabilization time with all types of detectors. The absence of a stationary phase
in the last part of the column significantly reduces background noise. The impact of water,
oxygen or other polar or aggressive components that move through the end of the column
at high temperature will also be greatly reduced.

EZ-Guard
Phase
VF-1ms

ID (mm)
0.20
0.25

Length (m)
12
30

VF-5ms

0.25

15
30

VF-Xms

0.53
0.25

60
30
30

VF-17ms

0.25

30

VF-1701ms

0.25

30

VF-35ms

0.25

30

Film (µm)
0.33
0.25
0.25
0.25
0.25
0.25
0.50
0.50
0.25
0.25
0.10
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25

Guard Length (m)
5
5
10
5
5
10
5
10
5
10
10
5
10
5
10
5
10
5
10

Part No.
CP9023
CP9010
CP9011
CP9021
CP9012
CP9013
CP9014
CP9015
CP9016
CP9020
CP9022
CP9018
CP9019
CP9024
CP9025
CP9176
CP9177
CP9026
CP9027

AGILENT J&W GC AND GC/MS COLUMNS

363

Low-bleed GC/MS Columns

Low-bleed GC/MS Columns
Tips & Tools
Agilent MS Certified Liners are
lot-tested with MSD and FID for
superior acid/base deactivation,
response linearity and peak symmetry.
Turn to page 262.

There is a rapidly increasing population of benchtop GC/MS instruments in analytical laboratories
that analyze a widening range of trace level, higher temperature samples. These samples require
increasingly inert, lower bleed, higher temperature columns. In response to this growing need,
Agilent Technologies designed several "ms" columns to chromatograph a broader range of low
level samples and generate lower bleed even at higher temperatures.

What makes an Agilent J&W low bleed column unique?
What makes an Agilent J&W low bleed column exceptional? Unique polymer chemistry and
proprietary surface deactivation, both of which have contributed to columns that adhere to the
tightest quality control specifications in the industry for bleed, inertness, selectivity and efficiency.
Agilent J&W "ms" columns utilize special surface deactivation and siloxane chemistries which
enhance the chromatographic performance of siloxane polymers.
While some of the GC/MS phases utilize different polymer chemistries, their selectivity mimics
the standard polysiloxane phases and offers the advantages of low column bleed and, in some
cases, extended temperature ranges.
The mass spectrum of septum bleed can look very much like GC column bleed, so the two are
often confused. An easy way to tell the two apart: column bleed will be a rise in the baseline, not
peaks. If you see bleed peaks, these generally come from lower quality septa or septa being
used beyond their operating limits. To minimize septa contributions to background bleed, use
quality Agilent BTO, Long Life, or Advanced Green septa.

364

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Low-bleed GC/MS Columns

DB-1ms

CH3
O

Si
CH3

100%

Structure of DB-1ms

• 100% Dimethylpolysiloxane
• Identical selectivity to DB-1
• Non-polar
• Very low bleed characteristics, ideal for GC/MS
• Improved acid performance compared to standard 100% Dimethylpolysiloxane columns
• Improved signal-to-noise ratio for better sensitivity and mass spectral integrity
• 340/360°C upper temperature limit
• Excellent general purpose column
• Bonded and cross-linked
• Solvent rinsable
DB-1ms Chromatograms
Life Sciences
Drug Screen

Page 673

DB-1ms
ID (mm)
0.10

Length (m)
10
20

0.18
0.20
0.25

0.32

20
12
25
15
30
60
15
30
60

Film (µm)
0.10
0.40
0.10
0.40
0.18
0.33
0.33
0.25
0.10
0.25
0.25
0.25
0.10
0.25
0.25

Temp Limits (°C)
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/350
-60 to 340/350
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/360

7 in. Cage
127-0112
127-0113
127-0122
127-0123
121-0122
128-0112
128-0122
122-0112
122-0131
122-0132
122-0162
123-0112
123-0131
123-0132
123-0162

5 in. Cage

121-0122E
128-0122E
122-0112E
122-0132E

7890/6890
LTM Module
127-0112LTM
127-0113LTM
127-0122LTM
127-0123LTM
121-0122LTM
128-0112LTM
128-0122LTM
122-0112LTM
122-0131LTM
122-0132LTM

5975T
LTM Toroid

221-0122LTM

222-0112LTM
222-0132LTM

123-0112LTM
123-0131LTM
123-0132LTM

AGILENT J&W GC AND GC/MS COLUMNS

365

Low-bleed GC/MS Columns

HP-1ms
CH3
O

• 100% Dimethylpolysiloxane
• Identical selectivity to HP-1
• Non-polar
• Low bleed characteristics
• Excellent general purpose column
• Improved signal-to-noise ratio for better sensitivity and mass spectral integrity
• Bonded and cross-linked
• Solvent rinsable

Si
CH3

100%

Structure of HP-1ms

HP-1ms Chromatograms
Environmental
Nitrogen Containing Herbicides (EPA Method 507)

Page 586

HP-1ms
ID (mm)
0.18
0.20
0.25

0.32

Length (m)
20
25
15
30

60
15
25
30
60

366

GC AND GC/MS

Film (µm)
0.18
0.33
0.25
0.10
0.25
0.50
1.00
0.25
0.25
0.52
0.25
1.00
0.25

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

7 in. Cage
19091S-677
19091S-602
19091S-931
19091S-833
19091S-933
19091S-633
19091S-733
19091S-936
19091S-911
19091S-612
19091S-913
19091S-713
19091S-916

5 in. Cage
19091S-677E
19091S-602E
19091S-931E
19091S-933E
19091S-733E
19091S-936E

19091S-913E

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7890/6890
LTM Module
19091S-677LTM
19091S-602LTM
19091S-931LTM
19091S-833LTM
19091S-933LTM
19091S-633LTM
19091S-733LTM
19091S-911LTM
19091S-612LTM
19091S-913LTM
19091S-713LTM

5975T
LTM Toroid
29091S-677LTM
29091S-931LTM
29091S-833LTM
29091S-433LTM

Low-bleed GC/MS Columns

VF-1ms

CH3
O

Si
CH3

100%

Structure of VF-1ms

• Lowest guaranteed bleed specification for trace analysis with MS
• Wide range of applications ensures near universal applicability
• Highly inert for accurate analysis, even at trace levels
VF-1ms is a highly inert, non-polar, low bleed GC column providing increased sensitivity over a
broad array of applications. The 100% dimethylpolysiloxane phase delivers a guaranteed bleed
specification of 1 pA @ 325°C (30 m, 0.25 mm, 0.25 µm).
The VF-1ms comes with an EZ-GRIP to simplify installation, coupling and operation of capillary
columns. For guaranteed performance, the retention index, efficiency, selectivity and bleed is
measured and specified on the test report supplied with every column.
VF-1ms is also available with 0.15 mm ID for fast GC and GC/MS that can double sample
throughput when compared to 0.25 and 0.32 mm ID columns.

VF-1ms Chromatograms
Food, Flavors and Fragrances
Triglycerides C28-C54
Separation of TMS-derivatized sugars

Page 639
Page 630

Column shown with EZ-GRIP

VF-1ms
ID (mm)
0.10

Length (m)
10
20

0.15

10
15
20

Film (µm)
0.10
0.40
0.10
0.40
0.15
0.15
0.15
0.60

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

7 in. Cage
CP8900
CP8901
CP8902
CP8903
CP9030
CP5881
CP9031
CP9032

5 in. Cage

CP9032I5
(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

367

Low-bleed GC/MS Columns

VF-1ms
ID (mm)
0.20
0.25

Length (m)
12
25
15

25
30

50
60
0.32

15

25
30

50
60
0.53

15
30

368

GC AND GC/MS

Film (µm)
0.33
0.33
0.10
0.25
1.00
0.25
0.40
0.10
0.25
1.00
0.25
0.40
0.25
1.00
0.10
0.25
1.00
0.25
0.40
0.10
0.25
0.50
1.00
0.25
0.40
0.25
1.00
0.50
1.50
0.50
1.00
1.50

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 310/335

7 in. Cage
CP8904
CP8905
CP8906
CP8907
CP8908
CP8909
CP8910
CP8911
CP8912
CP8913
CP8914
CP8915
CP8916
CP8917
CP8918
CP8919
CP8920
CP8921
CP8922
CP8923
CP8924
CP8925
CP8926
CP8927
CP8928
CP8929
CP8930
CP8965
CP8967
CP8968
CP8969
CP8970

5 in. Cage

CP8906I5
CP8907I5
CP8908I5

CP8911I5
CP8912I5
CP8913I5

CP8916I5
CP8917I5
CP8918I5
CP8920I5

Low-bleed GC/MS Columns

DB-5ms
O

CH3

CH3

Si

Si

CH3

CH3

CH3
O
m

Structure of DB-5ms

Si
CH3

n

• Phenyl Arylene polymer virtually equivalent to a (5%-Phenyl)-methylpolysiloxane
• Non-polar
• Very low bleed characteristics, ideal for GC/MS
• Excellent inertness for active compounds
• Improved signal-to-noise ratio for better sensitivity and mass spectral integrity
• Bonded and cross-linked
• Solvent rinsable
• MSD testing and certification available
• Exact replacement of HP-5TA
• Close equivalent to USP Phase G27
• Test mix available
DB-5ms Chromatograms
Environmental
Diesel Fuel
EPA Air Analysis Method TO-15 (1 ppbV Standard)
EPA Method 525.2
EPA Method 8061 (Phthalate Esters)
Formaldehyde, 50ppb
Organochlorine Pesticides II EPA Method 8081A
Organophosphorous Pesticides I, EPA Method 8141A
Phenols
Sulfur in Air

Page 570
Page 612
Page 598
Page 599
Page 613
Page 575
Page 583
Page 601
Page 613

Industrial Chemicals
Amines and Nitriles
Phenols II
Polyethyleneamines
Substituted Anilines

Page 646
Page 666
Page 646
Page 665

Life Sciences
Narcotics

Page 679

AGILENT J&W GC AND GC/MS COLUMNS

369

Low-bleed GC/MS Columns

DB-5ms
ID (mm)
0.18

0.20

0.25

Length (m)
20
40
12
25
50
15

25
30

50
60

0.32

15

25
30

60

0.53

370

15
30

GC AND GC/MS

Film (µm)
0.18
0.36
0.18
0.33
0.33
0.33
0.10
0.25
0.50
1.00
0.25
0.40
0.10
0.25
0.50
1.00
0.25
0.10
0.25
1.00
0.10
0.25
1.00
0.52
0.10
0.25
0.50
1.00
0.10
0.25
0.50
1.00
1.50
0.50
1.00
1.50

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 300/320
-60 to 300/320
-60 to 300/320
-60 to 300/320

7 in. Cage
121-5522
121-5523
121-5542
128-5512
128-5522
128-5552
122-5511
122-5512
122-5516
122-5513
122-5522
122-552a
122-552A
122-5532
122-5536
122-5533
122-5552
122-5561
122-5562
122-5563
123-5511
123-5512
123-5513
123-5526
123-5531
123-5532
123-5536
123-5533
123-5561
123-5562
123-5566
123-5563
125-5512
125-5537
125-553J
125-5532

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

5 in. Cage
121-5522E

7890/6890
LTM Module
121-5522LTM
121-5523LTM

5975T
LTM Toroid
221-5522LTM

128-5512LTM
128-5522LTM

122-5532E
122-5536E
122-5533E

122-5511LTM
122-5512LTM
122-5516LTM
122-5513LTM
122-5522LTM
122-552aLTM
122-552ALTM
122-5532LTM
122-5536LTM
122-5533LTM

122-5562E

123-5513E

123-5532E

123-5511LTM
123-5512LTM
123-5513LTM
123-5526LTM
123-5531LTM
123-5532LTM
123-5536LTM
123-5533LTM

125-5512LTM
125-5537LTM
125-553JLTM
125-5532LTM

222-5512LTM

222-5532LTM

Low-bleed GC/MS Columns

HP-5ms
CH6H5

CH3
O

Si
CH3

O
95%

Si
CH6H5

Structure of HP-5ms

5%

• (5%-Phenyl)-methylpolysiloxane
• Identical selectivity to HP-5
• Non-polar
• Very low bleed characteristics, ideal for GC/MS
• Excellent inertness for active compounds including acidic and basic compounds
• Improved signal-to-noise ratio for better sensitivity and mass spectral integrity
• Bonded and cross-linked
• Solvent rinsable
• Equivalent to USP Phase G27
HP-5ms Chromatograms
Environmental
Chlorinated Pesticides, EPA Method 508
Nitrogen/Phosphorus Containing Pesticides, EPA Method 507
Organohalide Pesticides in Water, EPA Method 505
Semivolatile Compounds, EPA Method 8270

Page 578
Page 582
Page 578
Page 597

Food, Flavors and Fragrances
Fragrance Allergens

Page 618

Industrial Chemicals
Trace Active Amines, 10 ng on-column
Phenols I

Page 645
Page 666

AGILENT J&W GC AND GC/MS COLUMNS

371

Low-bleed GC/MS Columns

HP-5ms
ID (mm)
0.18
0.20

0.25

Length (m)
20
12
25
50
15

30

60
0.32

25
30

60

372

GC AND GC/MS

Film (µm)
0.18
0.33
0.33
0.33
0.10
0.25
1.00
0.10
0.25
0.50
1.00
0.10
0.25
0.52
0.10
0.25
0.50
1.00
0.25

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

7 in. Cage
19091S-577
19091S-101
19091S-102
19091S-105
19091S-331
19091S-431
19091S-231
19091S-333
19091S-433
19091S-133
19091S-233
19091S-336
19091S-436
19091S-112
19091S-313
19091S-413
19091S-113
19091S-213
19091S-416

5 in. Cage

19091S-102E

19091S-433E
19091S-233E
19091S-436E
19091S-112E
19091S-413E

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7890/6890
LTM Module
19091S-577LTM
19091S-101LTM
19091S-102LTM
19091S-331LTM
19091S-431LTM
19091S-231LTM
19091S-333LTM
19091S-433LTM
19091S-133LTM
19091S-233LTM

19091S-112LTM
19091S-313LTM
19091S-413LTM
19091S-113LTM
19091S-213LTM

5975T
LTM Toroid

29091S-433LTM

Low-bleed GC/MS Columns

O

CH3

CH3

Si

Si

CH3

CH3

VF-5ms

CH3
O
m

Structure of VF-5ms

Si
CH3

n

• Excellent selectivity for aromatic compounds
• Minimal column bleed improves sensitivity
• Individual test certificates guarantee performance
VF-5ms is a highly inert 5% phenyl-methyl column for increased sensitivity, accuracy
and instrument uptime. The columns have the lowest guaranteed bleed specification of
1 pA @ 325°C (30 m, 0.25 mm, 0.25 µm). VF-5ms has a slightly higher polarity than VF-1ms,
resulting in a better selectivity for aromatic compounds. This selectivity, combined with superior
inertness, also makes these columns applicable for a wide range of semi-polar and even polar
components, such as phenols.
VF-5ms is also available with 0.15 mm ID for fast GC and GC/MS that can at least double sample
throughput when compared to 0.25 and 0.32 mm ID columns.

VF-5ms Chromatograms
Environmental
High resolution phenol analysis by GC/MS

Page 602

Food, Flavors and Fragrances
Pesticides in sunflower oil

Page 640

VF-5ms
ID (mm)
0.10
0.15

Length (m)
10
20
10
15
20

40
0.20

12
25
50

Film (µm)
0.40
0.40
0.15
0.15
0.15
0.30
0.60
0.15
0.60
0.33
0.33
0.33

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

7 in. Cage
CP8934
CP8933
CP9034
CP9035
CP9036
CP9037
CP9038
CP9039
CP9040
CP8935
CP8936
CP8937

5 in. Cage

CP9034I5
CP9036I5

CP9039I5
CP8935I5
CP8936I5

(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

373

Low-bleed GC/MS Columns

VF-5ms
ID (mm)
0.25

Length (m)
15

25
30

50
60

0.32

15

25
30

50
60
0.53

15

30

374

GC AND GC/MS

Film (µm)
0.10
0.25
0.50
1.00
0.25
0.40
0.10
0.25
0.50
1.00
0.25
0.10
0.25
1.00
0.10
0.25
1.00
0.52
0.10
0.25
0.50
1.00
0.25
0.40
0.25
1.00
0.50
1.00
1.50
0.50
1.00
1.50

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 310/335

7 in. Cage
CP8938
CP8939
CP8963
CP8940
CP8941
CP8942
CP8943
CP8944
CP8945
CP8946
CP8947
CP8948
CP8960
CP8949
CP8950
CP8951
CP8952
CP8953
CP8954
CP8955
CP8956
CP8957
CP8958
CP8959
CP8961
CP8962
CP8971
CP8972
CP8973
CP8974
CP8975
CP8976

5 in. Cage

CP8941I5
CP8943I5
CP8944I5

CP8948I5
CP8960I5

CP8954I5
CP8955I5
CP8957I5

CP8961I5

Low-bleed GC/MS Columns

DB-XLB
• EXceptionally Low Bleed
• Low polarity
• Extended temperature limit of 340/360°C
• Unique selectivity
• Excellent inertness for active compounds
• Ideal for confirmational analyses
• Excellent for pesticides, herbicides, PCBs and PAHs
• Ideal for GC/MS
• MSD testing and certification available
• Bonded and cross-linked
• Solvent rinsable
Note: "DB-XLB is designed for inhibiting column bleed at high temperatures.
It also appears to have inadvertently inherited an exceptional ability for separating many
PCB congeners when used with MS detection. This stellar performance was maximized
after careful optimization of the column dimensions, temperature programs, and carrier
gas flow conditions."
(Frame, G. Analytical Chemistry News & Features, Aug. 1, 1997, 468A-475A)

DB-XLB Chromatograms
Environmental
Aroclors 1016-1268 (without 1221)
CLP Pesticides
CLP Pesticide Analysis
Congeners in DIN Method PCBs
EPA Method 552.2
Herbicides I
PBDEs
PCBs by EPA Method 8082
Pesticides, EPA 508.1
Phenols
Phenoxy Acid Herbicides – Methyl Derivatives, EPA 8151A

Page 592
Page 574
Page 573
Page 593
Page 603
Page 585
Page 592
Page 594
Page 577
Page 601
Page 584

Food, Flavors and Fragrances
Ylang Ylang Oil

Page 626

AGILENT J&W GC AND GC/MS COLUMNS

375

Low-bleed GC/MS Columns

DB-XLB
ID (mm)
0.18
0.20
0.25

0.32

0.53

376

GC AND GC/MS

Length (m) Film (µm)
20
0.18
30
0.18
12
0.33
25
0.33
15
0.10
0.25
30
0.10
0.25
0.50
1.00
60
0.25
30
0.25
0.50
60
0.25
15
1.50
30
1.50

Temp Limits (°C)
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 320/340
30 to 320/340

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7 in. Cage
121-1222
121-1232
128-1212
128-1222
122-1211
122-1212
122-1231
122-1232
122-1236
122-1233
122-1262
123-1232
123-1236
123-1262
125-1212
125-1232

7890/6890
5 in. Cage LTM Module
121-1222E 121-1222LTM
121-1232LTM
128-1212E 128-1212LTM
128-1222LTM
122-1211LTM
122-1212LTM
122-1231LTM
122-1232LTM
122-1236LTM
122-1233LTM
122-1262E
123-1232LTM
123-1236LTM
125-1212LTM
125-1232LTM

Low-bleed GC/MS Columns

VF-Xms
CH3
O Si
CH3

CH3
Si
CH3

CH3
O Si
CH3
X

Structure of VF-Xms

Y

• High arylene modified phase for accurate results
• Isothermal applications up to 340°C for a broad application range
• Ideal for confirmational analysis for ultimate confidence
The VF-Xms has the lowest bleed of all FactorFour columns. VF-Xms delivers the ultimate in
sensitivity and signal-to-noise ratio, and is the low bleed, more polar alternative to the VF-5ms.
Compared to non-polar "ms" type phases, VF-Xms provides exceptionally high selectivity for
pesticides and delivers high resolution in the shortest analysis time.
The VF-Xms comes with an EZ-GRIP, simplifying installation, coupling and operation of capillary
columns. For guaranteed performance, the retention index, efficiency, selectivity and bleed is
measured and specified on the test report supplied with each column.

VF-Xms Chromatograms
Environmental
Analysis of Polycyclic Aromatic Hydrocarbons
Column shown with EZ-GRIP

Page 570

VF-Xms
ID (mm)
0.15
0.20
0.25

Length (m)
20
12
25
15
30

0.32

60
15
30

0.53

60
15
30

Film (µm)
0.15
0.33
0.33
0.10
0.25
0.10
0.25
0.50
1.00
0.25
0.25
1.00
0.10
0.25
0.50
1.00
0.25
1.50
1.50

Temp Limits (°C)
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 325/340
30 to 325/340

7 in. Cage
CP9041
CP8800
CP8801
CP8802
CP8803
CP8805
CP8806
CP8807
CP8808
CP8809
CP8810
CP8811
CP8812
CP8813
CP8814
CP8815
CP8816
CP8817
CP8818

5 in. Cage

CP8806I5

AGILENT J&W GC AND GC/MS COLUMNS

377

Low-bleed GC/MS Columns

DB-35ms
O

CH3

CH3

Si

Si

CH3

CH3

CH3
O

Si
CH3

m

Structure of DB-35ms

n

• Virtually equivalent to a (35%-Phenyl)-methylpolysiloxane
• Mid-polarity
• Very low bleed characteristics, ideal for GC/MS
• Extended temperature limit of 340/360°C
• Excellent inertness for active compounds
• Ideal for confirmational analyses
• Bonded and cross-linked
• Solvent rinsable
• Replaces HP-35ms
• Close equivalent to USP Phase G42
DB-35ms Chromatograms

378

GC AND GC/MS

Environmental
CLP Pesticides
EPA Method 552.2
Organochlorine Pesticides I EPA Method 8081A
Organophosphorous Pesticides I, EPA Method 8141A
PCBs by EPA Method 8082
Pesticides, EPA 508.1
Phenoxy Acid Herbicides – Methyl Derivatives, EPA 8151A

Page 574
Page 603
Page 575
Page 583
Page 594
Page 577
Page 584

Industrial Chemicals
Anilines

Page 665

Life Sciences
Barbiturates
Benzodiazepines II

Page 677
Page 677

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Low-bleed GC/MS Columns

DB-35ms
ID (mm)
0.18
0.20
0.25

0.32
0.53

Length (m)
20
15
25
15
30
60
15
30
30

Film (µm)
0.18
0.33
0.33
0.25
0.15
0.25
0.25
0.25
0.25
0.50
1.00

Temp Limits (°C)
50 to 340/360
50 to 340/360
50 to 340/360
50 to 340/360
50 to 340/360
50 to 340/360
50 to 340/360
50 to 340/360
50 to 340/360
50 to 320/340
50 to 320/340

7 in. Cage
121-3822
128-3812
128-3822
122-3812
122-3831
122-3832
122-3862
123-3812
123-3832
125-3837
125-3832

5 in. Cage
121-3822E

122-3832E

123-3832E

7890/6890
LTM Module
121-3822LTM
128-3812LTM
128-3822LTM
122-3812LTM
122-3831LTM
122-3832LTM

5975T
LTM Toroid
221-3822LTM

222-3812LTM
222-3832LTM

123-3812LTM
123-3832LTM
125-3837LTM
125-3832LTM

AGILENT J&W GC AND GC/MS COLUMNS

379

Low-bleed GC/MS Columns

VF-35ms
O Si

CH3
O Si
CH3

CH3
Si
CH3

35%

Structure of VF-35ms

CH3
O Si
CH3
X

Y

• Ideal for dual column confirmational analysis for ultimate confidence
• High maximum temperature for broad applicability
• Stabilized arylene-modified equivalent of a 35% phenylmethyl phase for longevity
The VF-35ms is a medium polarity column, which is the ideal choice for trace environmental and
chemical analyses, and as a confirmation column. The VF-35ms uses FactorFour technology to
produce a low bleed, highly stable column with a programmable maximum temperature of
360°C.
VF-35ms is also available with 0.15 mm ID for fast GC and GC/MS that can double sample
throughput when compared to 0.25 and 0.32 mm ID columns.

VF-35ms Chromatograms
Environmental
Organochlorine pesticides to EPA 625 via GC/MS

Page 588

VF-35ms
ID (mm)
0.15

0.20
0.25

0.32

0.53

380

GC AND GC/MS

Length (m)
10
15
20
15
25
15
30

60
15
30

60
15
30

Film (µm)
0.15
0.15
0.15
0.33
0.33
0.25
0.10
0.15
0.25
0.50
1.00
0.25
0.25
0.25
0.50
1.00
0.25
1.00
0.50
1.00

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 325/350
40 to 325/350
40 to 325/350

7 in. Cage
CP5887
CP5888
CP5889
CP8872
CP8873
CP8874
CP8875
CP8876
CP8877
CP8878
CP8879
CP8880
CP8881
CP8882
CP8883
CP8884
CP8885
CP8886
CP8887
CP8888

5 in. Cage

CP8877I5
CP8878I5

CP8883I5

Low-bleed GC/MS Columns

DB-17ms
O

CH 3

CH 3

Si

Si

CH 3

CH 3

CH 3
O
m

Structure of DB-17ms

Si
CH 3

n

• Virtually equivalent to (50%-Phenyl)-methylpolysiloxane
• 320/340°C upper temperature limit
• Very low bleed mid-polarity column, ideal for GC/MS
• Excellent inertness for active compounds
• Enhanced mass spectral integrity
• Bonded and cross-linked
• Solvent rinsable
• Best column for CLP pesticides
DB-17ms Chromatograms
Environmental
PAHs

Page 600

Life Sciences
Hallucinogens
Tocopherols

Page 678
Page 678

DB-17ms
ID (mm)
0.18
0.25

Length (m)
20
15
30

0.32

60
15
30

Film (µm)
0.18
0.15
0.25
0.15
0.25
0.25
0.25
0.25

Temp Limits (°C)
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340

7 in. Cage
121-4722
122-4711
122-4712
122-4731
122-4732
122-4762
123-4712
123-4732

5 in. Cage
121-4722E

122-4732E

7890/6890
LTM Module
121-4722LTM
122-4711LTM
122-4712LTM
122-4731LTM
122-4732LTM

5975T
LTM Toroid
221-4722LTM
222-4712LTM
222-4732LTM

123-4712LTM
123-4732LTM

AGILENT J&W GC AND GC/MS COLUMNS

381

Low-bleed GC/MS Columns

VF-17ms
CH3
O Si

O Si
CH3
X

Structure of VF-17ms

Y

• Deactivation technology improves data quality
• Ideal EPA confirmation column for ultimate confidence
• Bonded and cross-linked to allow solvent rinsing, reducing replacement costs
VF-17ms is a 50% phenyl, 50% dimethylpolysiloxane, medium polarity, low bleed column for
increased sensitivity, accuracy and instrument uptime. VF-17ms is often referenced in
environmental and clinical methods. The use of new deactivation technology improves column
stability, resulting in improved repeatability and column lifetimes. VF-17ms has a very low bleed
specification at 2 pA @ 325°C (0.25 mm x 30 m x 0.25 µm).
VF-17ms is also available with 0.15 mm ID for fast GC and GC/MS that can double sample
throughput when compared to 0.25 and 0.32 mm ID columns.

VF-17ms Chromatograms
Environmental
Organochlorine pesticides

Page 582

VF-17ms
ID (mm)
0.10
0.15

0.25

0.32

Length (m)
10
10
15
20
15
15
30

60
15
30

0.53

15

30

382

GC AND GC/MS

Film (µm)
0.20
0.15
0.15
0.15
0.25
0.50
0.15
0.25
0.50
0.25
0.15
0.25
0.25
0.50
0.25
1.00
1.50
0.50
1.00
1.50

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 330/360
40 to 310/340
40 to 330/360
40 to 310/340
40 to 310/340

7 in. Cage
CP8977
CP5882
CP5883
CP5884
CP8979
CP8980
CP8981
CP8982
CP8983
CP8984
CP8986
CP8987
CP8990
CP8991
CP8994
CP8996
CP8998
CP9000
CP9001
CP9002

5 in. Cage

CP8982I5

CP8990I5

Low-bleed GC/MS Columns

C N
(CH2)3
O Si
(CH2)3
C N

VF-23ms
CH3
O Si
CH3

CH3
Si
CH3
Y

X

Structure of VF-23ms

• 100% bonded phase permits column rinsing to enhance column lifetime
• Fast run times improve productivity
• Operating temperature up to 260°C expands the application range
The VF-23ms column has a high polarity and highly substituted cyanopropyl low bleed phase.
VF-23ms features a unique combination of high polarity and low bleed to enable more accurate
analysis of very polar analytes. The enhanced stabilization of the VF-23ms permits splitless
injection, column rinsing and temperatures up to 260°C to be used. Compared to other 23ms
type phases, this expands the range of possible applications by enabling the analysis of higher
molecular weight compounds.
VF-23ms is also available with 0.15 mm ID for fast GC and GC/MS that can double sample
throughput when compared to 0.25 and 0.32 mm ID columns.

VF-23ms Chromatograms
Food, Flavors and Fragrances
Fast screening of FAME isomers in butter

Page 639

VF-23ms
ID (mm)
0.10
0.15

0.25

Length (m)
10
15
20
40
15
30
60

0.32

15
30
60

0.53

15
30

Film (µm)
0.10
0.15
0.15
0.15
0.25
0.15
0.25
0.15
0.25
0.25
0.15
0.25
0.15
0.25
0.50
0.50

Temp Limits (°C)
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 260/260
40 to 245/245
40 to 245/245

7 in. Cage
CP8819
CP5886
CP9042
CP5885
CP8820
CP8821
CP8822
CP8823
CP8824
CP8825
CP8826
CP8827
CP8828
CP8829
CP8830
CP8831

5 in. Cage

CP8820I5
CP8821I5
CP8822I5
CP8824I5

AGILENT J&W GC AND GC/MS COLUMNS

383

Low-bleed GC/MS Columns

VF-200ms

CF3
CH2
CH2

CH3

O Si
CH3

O Si
CH3

• Superior deactivation delivers symmetrical peaks to improve data accuracy
• Ultra-low background noise for trace analysis maximizes sensitivity
• Ideal for sensitive and selective detector systems for enhanced productivity

X

Y

Structure of VF-200ms

The VF-200ms is designed with a unique selectivity for compounds rich in dipole-dipole
interactions, resulting from the electrophilic nature of the trifluoropropyl stationary phase.
VF-200ms is especially suited for electron rich, high dipole moment compounds like ketones,
aldehydes, nitro- or chloro-containing compounds, PAHs, unsaturated compounds, silanes
and CFCs. VF-200ms, as with all FactorFour columns, offers superior surface deactivation
and thereby symmetrical peak shapes. The high inertness of the VF-200ms leads to more
accurate peak identification and reliable analysis. The VF-200ms trifluoropropyl phase has
very high temperature stability and can be used routinely up to 350°C.
VF-200ms is also available with 0.15 mm ID for fast GC and GC/MS that can double sample
throughput when compared to 0.25 and 0.32 mm ID columns.

VF-200ms Chromatograms
Industrial Chemicals
Fast separation of silanes

Page 670

VF-200ms
ID (mm)
0.15

Length (m)
10
20

0.25

15
30

0.32

0.53

384

GC AND GC/MS

60
15
30

15
30

Film (µm)
0.15
0.15
0.60
0.25
0.50
0.10
0.25
0.50
1.00
0.25
0.25
0.25
0.50
1.00
1.00
0.50
1.00

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 325/350
0 to 300/325
0 to 300/325
0 to 300/325

7 in. Cage
CP5893
CP5891
CP5892
CP8855
CP8856
CP8857
CP8858
CP8859
CP8860
CP8861
CP8862
CP8863
CP8864
CP8865
CP8866
CP8867
CP8868

5 in. Cage

CP8855I5

CP8859I5
CP8860I5

CP8865I5

CP8868I5

Low-bleed GC/MS Columns

DB-225ms
• Virtually equivalent to (50%-Cyanopropylphenyl)-methylpolysiloxane
• Mid/high polarity
• Excellent for separations of cis- and trans-fatty acid methyl esters (FAMEs)
• Low bleed
• Bonded and cross-linked
• Solvent rinsable
• Close equivalent to USP Phase G7
DB-225ms Chromatograms
Environmental
Tetrachlorodibenzo-p-furans

Page 591

Food, Flavors and Fragrances
FAMEs II

Page 633

DB-225ms
ID (mm)
0.25

0.32

Length (m)
15
30
60
30

Film (µm)
0.25
0.25
0.25
0.25

Temp Limits (°C)
40 to 240
40 to 240
40 to 240
40 to 240

7 in. Cage
122-2912
122-2932
122-2962
123-2932

5 in. Cage
122-2932E

7890/6890
LTM Module
122-2912LTM
122-2932LTM

5975T
LTM Toroid
222-2912LTM
222-2932LTM

123-2932LTM

AGILENT J&W GC AND GC/MS COLUMNS

385

Low-bleed GC/MS Columns

VF-WAXms
O CH2

CH2

OH
n

Structure of VF-WAXms

• Specially designed for MS for more accurate results with polar compounds
• Operating temperature range of 20°C to 250°C for maximum flexibility
• Better signal-to-noise ratio for trace analyses improves productivity
The VF-WAXms is a high performance column for applications in the food, flavors and fragrances
markets, and especially where trace analyses are required. These applications often require
higher temperatures to analyze polar compounds, and therefore need an ultra-stable wax as
a stationary phase. The very low bleed of VF-WAXms provides increased sensitivity, extended
column lifetime and greater accuracy, even at higher temperatures.
Advanced coating technology means that VF-WAXms columns are highly inert. Such inertness
gives better chromatograms, enhancing critical pair separation. With the introduction of the
VF-WAXms column, wax applications such as food, flavors and fragrances can now benefit from
the use of GC/MS detectors. Impurities can easily be identified using an MS detector when a
wax column is required for separation. Significantly improved performance is achieved with
VF-WAXms columns, yet the typical selectivity of PEG is unchanged.
VF-WAXms is also available with 0.15 mm ID for fast GC and GC/MS that can double sample
throughput when compared to 0.25 and 0.32 mm ID columns.

VF-WAXms Chromatograms
Food, Flavors and Fragrances
Acids

386

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Page 631

Low-bleed GC/MS Columns

Tips & Tools
As a special MS-type phase,
the VF-WAXms column generates
less bleed, and therefore less noise
and higher signal-to-noise ratios
for critical components.

VF-WAXms
ID (mm)
0.10

0.15

0.25

Length (m)
10
20
10
15
20
30
15
25
30

60
0.32

15

30

60

0.53

15
30
60

Film (µm)
0.10
0.20
0.10
0.15
0.15
0.15
0.15
0.25
0.50
0.20
0.25
0.50
1.00
0.25
0.50
0.25
0.50
1.00
0.25
0.50
1.00
0.25
0.50
1.00
1.00
2.00
1.00
2.00
1.00
2.00

Temp Limits (°C)
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 240
20 to 250/260
20 to 240
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 240
20 to 250/260
20 to 240
20 to 230
20 to 250/260
20 to 240
20 to 240
20 to 230
20 to 230
20 to 220

7 in. Cage
CP9219
CP9218
CP9229
CP9200
CP9201
CP9220
CP9202
CP9203
CP9221
CP9204
CP9205
CP9222
CP9206
CP9207
CP9223
CP9209
CP9224
CP9208
CP9212
CP9210
CP9211
CP9214
CP9225
CP9213
CP9226
CP9227
CP9215
CP9216
CP9228
CP9217

5 in. Cage

CP9229I5

CP9205I5

CP9212I5

CP9225I5
CP9226I5

AGILENT J&W GC AND GC/MS COLUMNS

387

Low-bleed GC/MS Columns

VF-624ms and VF-1301ms
C N
(CH2)3
O Si
CH3
X

CH3
O Si
CH3

CH3
O Si
CH3

CH3
Si
CH3

Y

Z

Structure of VF-624ms and VF-1301ms

• Improved signal-to-noise ratio for more accurate trace analysis
• Eliminate ghost peaks and unstable baselines for best data accuracy
• Enhanced selectivity eliminating co-eluters such as benzene and 1,2-dichloroethane for
improved productivity

The VF-624ms and VF-1301ms are the world's first ultra-low bleed 6% cyanopropyl/phenyl,
94% PDMS GC columns. VF-624ms columns set a new standard for the analysis of volatile
organic compounds. Improved phase technology reduces bleed, thereby increasing signal-tonoise ratios. These columns are especially suited for analyzing solvents according to EPA
Methods 524, 624 and 8260, as well as USP 467.
The ultra low bleed, thin filmed, VF-1301ms column has a similar selectivity and is suitable for
semi-volatile organic solvents, as well as PCBs and pesticides.
VF-624ms and VF-1301ms are also available with 0.15 mm ID for fast GC and GC/MS that can
boost sample throughput when compared to 0.25 and 0.32 mm ID columns.

VF-624ms and VF-1301ms Chromatograms
Environmental
FactorFour cyano columns eliminate unstable baselines

Page 610

VF-624ms
ID (mm)
0.15

0.25
0.32
0.53

388

GC AND GC/MS

Length (m)
15
20
30
40
30
60
30
60
30
60
75

Film (µm)
0.84
0.84
0.84
0.84
1.40
1.40
1.80
1.80
3.00
3.00
3.00

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 265/280
-40 to 265/280

7 in. Cage
CP9101
CP9100
CP9109
CP9110
CP9102
CP9103
CP9104
CP9105
CP9106
CP9107
CP9108

5 in. Cage
CP9101I5

CP9102I5
CP9103I5
CP9104I5
CP9105I5
CP9106I5

Low-bleed GC/MS Columns

VF-1301ms
ID (mm)
0.10
0.15
0.25

Length (m)
10
15
20
15
30
60

0.32

15
30

0.53

60
15
30

Film (µm)
1.00
0.15
0.15
1.00
0.25
1.00
0.25
1.00
0.25
1.00
0.25
1.00
1.00
1.00
1.00
1.50

Temp Limits (°C)
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300
-40 to 280/300

7 in.
Cage
CP9066
CP9050
CP9051
CP9052
CP9053
CP9054
CP9055
CP9056
CP9057
CP9058
CP9059
CP9060
CP9061
CP9062
CP9063
CP9064

5 in.
Cage

CP9060I5

AGILENT J&W GC AND GC/MS COLUMNS

389

Low-bleed GC/MS Columns

VF-1701ms
C N
(CH2)3
O Si
CH3
X

CH3
O Si
CH3

CH3
O Si
CH3

CH3
Si
CH3

Y

Z

Structure of VF-1701ms

• Highly inert for difficult analytes such as p,p’-DDT to improve productivity
• Column deactivation for more accurate trace analysis
• Eliminate ghost peaks and unstable baselines for more reliable data
The VF-1701ms is the world’s first ultra-low bleed 14% cyanopropyl/phenyl, 86% PDMS
GC column for pesticides, PCBs and semi-volatile organic compounds. Improved phase
technology delivers increased inertness and reduced bleed, resulting in more accurate trace
analysis. The bleed specification is 2 pA @ 280°C for a 0.25 mm x 60 m x 0.25 µm ID column.
VF-1701ms is also available with 0.15 mm ID for fast GC and GC/MS that can double sample
throughput when compared to 0.25 and 0.32 mm ID columns.

VF-1701ms Chromatograms
Environmental
Analysis of pesticides using EPA 8081 with ECD

390

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Page 576

Low-bleed GC/MS Columns

VF-1701ms
ID (mm)
0.10

Length (m)
10

0.15

20
15

20
0.25

15

30

60

0.32

15

30

60

0.53

15
30

60

Film (µm)
0.20
0.40
0.10
0.10
0.15
0.60
0.15
0.60
0.15
0.25
1.00
0.15
0.25
1.00
0.15
0.25
0.50
1.00
0.15
0.25
1.00
0.10
0.15
0.25
1.00
0.15
0.25
1.00
1.00
0.10
0.25
0.50
1.00
1.50
1.00
1.50

Temp Limits (°C)
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 280/300
-20 to 265/280

7 in. Cage
CP9140
CP9141
CP9142
CP9175
CP9143
CP9144
CP9145
CP9146
CP9147
CP9148
CP9149
CP9150
CP9151
CP9152
CP9153
CP9154
CP9155
CP9156
CP9157
CP9158
CP9159
CP9160
CP9161
CP9162
CP9163
CP9164
CP9165
CP9166
CP9167
CP9168
CP9169
CP9170
CP9171
CP9172
CP9173
CP9174

5 in. Cage

CP9149I5
CP9151I5
CP9152I5
CP9154I5
CP9155I5

CP9166I5

AGILENT J&W GC AND GC/MS COLUMNS

391

Polysiloxane Polymers Columns

Polysiloxane Polymers Columns
Polysiloxanes are the most common stationary phases. They are available in the greatest variety
and are stable, robust and versatile. Standard polysiloxanes are characterized by the repeating
siloxane backbone. Each silicon atom contains two functional groups. The type and amount of
the groups distinguish each stationary phase and its properties.
With the merger of Agilent and J&W Scientific there were many similar columns with the same
type of polymer. In some cases the manufacturing and QC processes were exactly the same. In
these cases the DB version was kept. In the cases where the HP and the DB columns had any
manufacturing or QC differences, we opted to keep both phases available, as in the case of DB-1
and HP-1. Each of these columns is a high-quality product made to meet exacting quality control
testing. However, there may be some subtle performance differences. For example the DB-35
and the HP-35 have slightly different selectivities. Therefore, we are still offering both DB and HP
versions for our customers who have methods already developed on these columns.

DB-1
CH3
O

Si
CH3

100%

Structure of DB-1

392

GC AND GC/MS

• 100% Dimethylpolysiloxane
• Non-polar
• Excellent general purpose column
• Wide range of applications
• Low bleed
• High temperature limit
• Bonded and cross-linked
• Solvent rinsable
• Wide range of column dimensions available
• Equivalent to USP Phase G2

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Polysiloxane Polymers Columns

DB-1 Chromatograms
Environmental
EPA Air Analysis Compendium Method TO-14 Standard
EPA Method 551
Pyrethrins

Page 611
Page 609
Page 595

Food, Flavors and Fragrances
Fragrance Reference Standard I
Spearmint Oil

Page 620
Page 617

Industrial Chemicals
Aldehydes and Ketones
Aromatics in Finished Gasoline – ASTM Method 5769
Esters I
PFBHA Derivative
Glycols III
Halogenated Hydrocarbons II
Nitrogen Based Solvents I
Triethylene Glycol and Impurities
Volatile Amines

Page 648
Page 702
Page 653
Page 649
Page 656
Page 658
Page 662
Page 656
Page 645

Life Sciences
Anabolic Steroids
Anticonvulsants

Page 681
Page 675

Petroleum
Aromatics in Finished Gasoline – ASTM Method 5769
DNPH Derivative
Polyethylene
Regular Unleaded Gasoline (California Phase 1) – "Normal" GC Run II
Volatile Sulfur Compounds

Page 702
Page 649
Page 706
Page 704
Page 694

AGILENT J&W GC AND GC/MS COLUMNS

393

Polysiloxane Polymers Columns

DB-1
ID (mm)
0.05

0.10

0.15
0.18

0.20

0.25

Length (m) Film (µm)
10
0.05
0.05
0.20
5
0.12
10
0.10
0.40
20
0.10
0.40
40
0.20
0.40
10
1.20
10
0.18
0.20
0.40
20
0.18
0.40
40
0.40
12
0.33
25
0.33
30
0.8
50
0.33
15
0.10
0.25
1.00
25
0.25
30
0.10
0.25
0.50
1.00
50
0.25
60
0.10
0.25
0.50
1.00
100
0.50
150
1.00

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

7 in.
Cage
126-1012
126-10SP
126-1013
127-100a
127-100A
127-1013
127-101A
127-1023
127-1046
127-1043
12A-1015
121-1012
121-101A
121-1013
121-1022
121-1023
121-1043
128-1012
128-1022
128-1034
128-1052
122-1011
122-1012
122-1013
122-1022
122-1031
122-1032
122-103E
122-1033
122-1052
122-1061
122-1062
122-106E
122-1063
122-10AE
122-10G3

5 in.
Cage

127-1012E
127-1013E
127-1022E
127-1023E
127-1046E

7890/6890
LTM Module
126-1012LTM
126-1013LTM
127-100aLTM
127-100ALTM
127-1013LTM
127-1022LTM
127-1023LTM

12a-1015LTM
121-1012E 12A-1015LTM
121-101aLTM
121-1013E 121-1013LTM
121-1022E 121-101ALTM
121-1023LTM
121-1043E
128-1012LTM
128-1022LTM
128-1034LTM
122-1011LTM
122-1012LTM
122-1013LTM
122-1022LTM
122-1031LTM
122-1032E 122-1032LTM*
122-103ELTM
122-1033E 122-1033LTM

*Also available as LTM column toroid assembly for Agilent 5975T, 0.25 mm x 30 m, 0.25 µm, P/N 222-1032LTM

(Continued)

394

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Polysiloxane Polymers Columns

DB-1
ID (mm)
0.32

Length (m) Film (µm)
10
0.5
15
0.10
0.25
1.00
3.00
5.00
25
0.12
0.25
0.52
1.05
30
0.10
0.25
0.50
1.00
1.50
3.00
5.00
50
0.25
0.52
1.05
1.20
5.00
60
0.10
0.25
0.50
1.00
1.50
2.00
3.00
5.00

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 280/300
-60 to 280/300
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 300/320
-60 to 280/300
-60 to 280/300
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 280/300
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 300/320
-60 to 280/300
-60 to 280/300
-60 to 280/300

7 in.
Cage
123-100E
123-1011
123-1012
123-1013
123-1014
123-1015
123-1027
123-1022
123-1026
123-102F
123-1031
123-1032
123-103E
123-1033
123-103B
123-1034
123-1035
123-1052
123-1056
123-105F
123-105C
123-1055
123-1061
123-1062
123-106E
123-1063
123-106B
123-106G
123-1064
123-1065

5 in.
Cage

7890/6890
LTM Module
123-100ELTM
123-1011LTM
123-1012LTM
123-1013LTM
123-1014LTM
123-1015LTM
123-1027LTM
123-1022LTM
123-1026LTM
123-102FLTM
123-1031LTM
123-1032LTM
123-103ELTM
123-1033E 123-1033LTM
123-103BLTM
123-1034LTM
123-1035LTM

123-1062E
123-1063E
123-106BE
123-1064E
123-1065E
(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

395

Polysiloxane Polymers Columns

DB-1
ID (mm)
0.45
0.53

396

GC AND GC/MS

Length (m) Film (µm)
30
1.27
2.55
5
0.88
2.65
5.00
7.5
1.50
10
2.65
5.00
15
0.15
0.25
0.50
1.00
1.50
3.00
5.00
25
1.00
5.00
30
0.10
0.25
0.50
1.00
1.50
2.65
3.00
5.00
50
5.00
60
1.00
1.50
3.00
5.00
105
5.00

Temp Limits (°C)
-60 to 325/350
-60 to 260/280
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 260/280
-60 to 260/280
-60 to 340/360
-60 to 320/340
-60 to 300/320
-60 to 300/320
-60 to 300/320
-60 to 260/280
-60 to 260/280
-60 to 300/320
-60 to 260/280
-60 to 340/360
-60 to 320/340
-60 to 300/320
-60 to 300/320
-60 to 300/320
-60 to 260/280
-60 to 260/280
-60 to 260/280
-60 to 260/280
-60 to 300/320
-60 to 300/320
-60 to 260/280
-60 to 260/280
-60 to 260/280

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7 in.
Cage
124-1032
124-1034
125-100a
125-100A
125-1005
125-1002
125-10HB
125-10H5
125-1011
125-101K
125-1017
125-101J
125-1012
125-1014
125-1015
125-102J
125-1025
125-1039
125-103K
125-1037
125-103J
125-1032
125-103B
125-1034
125-1035
125-1055
125-106J
125-1062
125-1064
125-1065
125-10B5

5 in.
Cage

125-10HBE
125-1011E

125-1012E

125-103KE

125-1034E
125-1035E
125-106JE
125-1062E
125-1065E

7890/6890
LTM Module
124-1032LTM
124-1034LTM
125-100aLTM
125-100ALTM
125-1005LTM
125-1002LTM
125-10HBLTM
125-10H5LTM
125-1011LTM
125-101KLTM
125-1017LTM
125-101JLTM
125-1012LTM
125-1014LTM
125-1015LTM
125-102JLTM
125-1025LTM
125-1039LTM
125-103KLTM
125-1037LTM
125-103JLTM
125-1032LTM
125-103BLTM
125-1034LTM
125-1035LTM

Polysiloxane Polymers Columns

HP-1
CH3
O

Si
CH3

100%

Structure of HP-1

• 100% Dimethylpolysiloxane
• Non-polar
• Excellent general purpose column – "Industry Standard"
• Wide range of applications
• Superior performance for low molecular weight alcohols (40°C reduces operating costs
• Temperature stability up to 300°C allows short regeneration times and improves efficiency
• One injector, one detector simplifies operation
Select Permanent Gases/CO2 is a set of two parallel columns that combine CP-Molsieve 5Å
for permanent gas analysis and CP-PoraBOND Q for CO2 analysis. The selection of column
dimensions accords with your need for fast separation, lowest level analysis, and quantification
of argon/oxygen. The Select Permanent Gases column separates permanent gases and CO2
in a single run, and columns are coupled, tested, and securely mounted on the EZ-GRIP
column mounting system.
The CP7429 Select Permanent Gases/CO2 column is designed for fast analysis of permanent
gases and CO2. For resolution of the difficult-to-separate argon/oxygen and helium/neon pairs,
use CP7430 Select Permanent Gases/HR (High Resolution) column.

Select Permanent Gases Chromatograms
Environmental
Fast analysis of permanent gases and CO2

Page 616

Select Permanent Gases
ID (mm)
Select Permanent Gases/CO2
Select Permanent Gases/HR

Temp Limits (°C)
300/325
300/325

7 in. Cage
CP7429
CP7430

AGILENT J&W GC AND GC/MS COLUMNS

499

Petroleum Columns

CP-Volamine
• Excellent stability for samples containing water expands the application range
• Maximum temperature of 265°C for enhanced productivity
• Highly inert providing sharp amine peaks for accurate results
CP-Volamine is optimized for the separation of volatile amines. The column is coated with a nonpolar stationary phase and produces symmetrical peaks due to MPD (Multi-Purpose
Deactivation) technology. CP-Volamine is the most stable column for analyzing volatile amines
even when the sample contains high percentages of water.
The CP-Volamine column is the best choice for analyzing volatile amines like MMA, DMA and
TMA (monomethyl, dimethyl and trimethyl amine). On this column other components of interest
such as alcohols, water, and ammonia also elute as sharp peaks. CP-Volamine is highly inert,
elutes a wide range of compounds, and delivers excellent performance and unique stability for
water. Both 15 m and 30 m columns are available to ensure the shortest run times for amine
samples that do not require the resolution of the 60 m column.

CP-Volamine Chromatograms
Industrial Chemicals
Amines and alcohols

Page 643

CP-Volamine
ID (mm)
0.32

Length (m)
15
30
60

Temp Limits (°C)
265/300
265/300
265/275

Tips & Tools
For fast and easy GC pressure and flow calculations at
your fingertips with Agilent's GC Calculator Application,
visit www.agilent.com/chem/GCapp

500

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7 in. Cage
CP7446
CP7447
CP7448

5 in. Cage
CP7447I5
CP7448I5

Petroleum Columns

CP-Sil 8 CB for Amines
• Good inertness towards basic compounds for best accuracy
• Guaranteed for the analysis of a broad range of amines for reliable results
• Available in non-polar and polar phases for broad application range
CP-Sil 8 CB for Amines is a base-deactivated 5% phenyl polydimethylsiloxane column that can
be used for a wide range of amines. Due to a thermal stability up to 350°C, it analyzes a broad
range of amines up to C20, as well as alkanolamines.

CP-Sil 8 CB for Amines Chromatograms
Industrial Chemicals
Analysis of ethanolamines

Page 643

CP-Sil 8 CB for Amines
ID (mm)
0.15
0.25
0.32
0.53

Length (m)
25
30
30
30
30

Film (µm)
2.00
0.25
0.50
1.00
1.00

Temp Limits (°C)
325/350
325/350
325/350
325/350
325/350

7 in. Cage
CP7599
CP7598
CP7595
CP7596
CP7597

5 in. Cage

Temp Limits (°C)
220/220
220/220

7 in. Cage
CP7422
CP7424

5 in. Cage
CP7422I5

Temp Limits (°C)
60 to 260/275

7 in. Cage
CP7405

5 in. Cage
CP7405I5

CP7598I5
CP7595I5
CP7596I5
CP7597I5

CP-Wax for Volatile Amines and Diamines
CP-Wax for Volatile Amines and Diamines
ID (mm)
0.32
0.53

Length (m)
25
25

Film (µm)
1.20
2.00

CP-Wax 51 for Amines
CP-Wax 51 for Amines
ID (mm)
0.25

Length (m)
25

Film (µm)
0.20

AGILENT J&W GC AND GC/MS COLUMNS

501

Petroleum Columns

CP-Lowox
• Unique selectivity for a wide range of oxygenates maximizes flexibility
• No particle loss preserves detector performance
• Suitable for process applications
CP-Lowox offers a unique solution to the chemical and petrochemical industries. It is now
possible to analyze trace level oxygenate impurities in gas and liquid hydrocarbon streams.
This high polarity makes the column ideal for the measurement of oxygenated compounds.
CP-Lowox can be used for the prevention of catalyst contamination by oxygenates,
process/on-line applications or portable GC applications (ASTM D 7059).

CP-Lowox Chromatograms
Petroleum
Analysis of oxygenates in a C1 to C5 hydrocarbon mix

Page 707

CP-Lowox
ID (mm)
0.53

Length (m)
10

Film (µm)
10.00

Temp Limits (°C)
0 to 350/350

7 in. Cage
CP8587

5 in. Cage
CP8587I5

GS-OxyPLOT
• Accurate analysis of ppm/ppb level oxygenates in C1 to C10 hydrocarbons
• Strong selectivity for a wide range of oxygenates (ethers, alcohols, aldehydes, and ketones)
in complex matrices such as gaseous hydrocarbons, motor fuels, and crude oil

• Suitable for ASTM methods for oxygenates
• Very high column stability (upper temperature limit of 350°C) with no column bleed
• Stable phase coating virtually eliminates particle generation and detector spiking
• Excellent for low concentration, quantitative GC analysis
• Ideal for selective heart-cutting applications
GS-OxyPLOT Chromatograms
Petroleum
Selected Oxygenates
Trace Oxygenates in Light Hydrocarbon Matrices

Page 686
Page 686

GS-OxyPLOT
ID (mm)
0.53

502

GC AND GC/MS

Length (m)
10

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
350

7 in. Cage
115-4912

5 in. Cage
115-4912E

Petroleum Columns

CP-Sil 5 CB for Formaldehyde
CP-Sil 5 CB for Formaldehyde
ID (mm)
0.32

Length (m)
60

Film (µm)
8.00

Temp Limits (°C)
-60 to 300/325

7 in. Cage
CP7475

5 in. Cage
CP7475I5

Film (µm)
0.20

Temp Limits (°C)
-15 to 90/95

7 in. Cage
CP7520

5 in. Cage
CP7520I5

Temp Limits (°C)
-60 to 300/325

7 in. Cage
CP7475

CP-Squalane
CP-Squalane
ID (mm)
0.25

Length (m)
100

CP-Propox
CP-Propox
ID (mm)
0.32

Length (m)
60

Film (µm)
8.00

Tips & Tools
View up-to-date educational resources such as posters,
Application Notes, training tools, seminars, product information
and more at www.agilent.com/chem/mygccolumns

AGILENT J&W GC AND GC/MS COLUMNS

503

Semivolatiles Columns

Semivolatiles Columns
Recommended Columns for Semivolatiles
• HP-5ms, DB-5.625, DB-5ms Ultra Inert, HP-5ms Ultra Inert for EPA methods 8270 and 525
• DB-XLB for PCB congeners
• HP-5ms, DB-5ms Ultra Inert, HP-5ms Ultra Inert or DB-35ms for PAHs
• DB-5ms, DB-5ms Ultra Inert or DB-XLB for phenols
Semivolatiles are usually extracted from soil samples or other environmental matrices. GC
columns with precise retention time reproducibility and good mass spectrometer performance are
key enablers for these often demanding analyses.

DB-Dioxin
• Specifically engineered for the analysis of polychlorinated dibenzodioxins (PCDDs) and
dibenzofurans (PCDFs)

• Resolves 2,3,7,8-TCDD and 2,3,7,8-TCDF from all other isomers in one run
• Low bleed
• Bonded and cross-linked
• Solvent rinsable
Note: 100 psi regulator required to reach optimum carrier gas velocity

DB-Dioxin

504

GC AND GC/MS

ID (mm)
0.25

Length (m)
60

0.32

60

Film (µm)
0.15
0.25
0.15
0.25

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
40 to 250/270
40 to 250/270
40 to 250/270
40 to 250/270

7 in. Cage
122-2461
122-2462
123-2461
123-2462

5 in. Cage
122-2461E

Semivolatiles Columns

CP-Sil 88 for Dioxins
• Integrated retention gap eliminates leaks and extends column lifetime
• 2,3,7,8-TCDD can be determined at low concentrations for ease-of-use
• Guaranteed analysis of dioxin isomers for complete confidence in results
The CP-Sil 88 column has a very high polarity and a specific selectivity for dioxins and
dibenzofuran separations. The column is supplied with an integrated retention gap to avoid
problems with solvent condensation, thus allowing repeated splitless injections without phase
deterioration, extending column life. In addition, because of the integrated retention gap, data
quality is considerably improved. For the shortest analysis times, a series of thin-film coated
columns is available that allow applications up to 270°C in temperature programmed mode.

CP-Sil 88 for Dioxins Chromatograms
Environmental
Dioxins and dibenzofurans

Page 571

CP-Sil 88 for Dioxins
ID (mm)
0.25

0.32

Length (m)
30
50
60
60

Film (µm)
0.10
0.20
0.10
0.13

Temp Limits (°C)
50 to 250/270
50 to 225/240
50 to 250/270
50 to 250/270

7 in. Cage
CP7497
CP7588
CP7498
CP7499

AGILENT J&W GC AND GC/MS COLUMNS

505

Semivolatiles Columns

DB-5.625
• Close equivalent to a (5%-Phenyl)-methylpolysiloxane
• Non-polar
• Specially processed to exhibit excellent inertness for EPA Semivolatiles Methods 625, 1625,
8270 and CLP protocols*

• Surpasses EPA performance criteria for semivolatiles
• Inert for base, neutral and acidic compounds
• High temperature limit with excellent thermal stability and low bleed
• Bonded and cross-linked
• Solvent rinsable
*Pentachlorophenol, 2,4-dinitrophenol, carbazole, and N-nitrosodiphenylamine used to test
response factors.

DB-5.625 Chromatograms
Environmental
Analysis of Semivolatiles
European Red List Volatiles

Page 591
Page 609

DB-5.625

506

GC AND GC/MS

ID (mm)
0.18

Length (m)
20

0.25

30

0.32

60
30

Film (µm)
0.18
0.36
0.25
0.50
1.00
0.25
0.25
0.50

Temp Limits (°C)
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7 in. Cage
121-5621
121-5622
122-5631
122-5632
122-5633
122-5661
123-5631
123-5632

7890/6890
LTM Module
121-5621LTM
121-5622LTM
122-5631LTM
122-5632LTM
122-5633LTM
123-5631LTM
123-5632LTM

Semivolatiles Columns

HP-5ms Semivolatile
• (5%-Phenyl)-methylpolysiloxane, identical selectivity to HP-5
• Non-polar
• Very low bleed characteristics, ideal for GC/MS
• Specifically tested for inertness for active compounds including acidic and basic compounds
• Improved signal-to-noise ratio for better sensitivity and mass spectral integrity
• Bonded and cross-linked
• Solvent rinsable
• Equivalent to USP Phase G27
HP-5ms Semivolatile
ID (mm)
0.25

Length (m) Film (µm)
30
0.50

Temp Limits (°C)
-60 to 325/350

7 in. Cage
19091S-139

7890/6890
LTM Module
19091S-139LTM

CP-Sil 5/C18 CB for PCB
• Guaranteed for very high resolution PCB analysis for ultimate confidence
• 100 m column separates critical isomer pairs for accurate results
• Use with high sensitivity ECD detection for enhanced productivity
CP-Sil 5/C18 CB for PCB has a lower polarity than 100% polydimethylsiloxane due to its C18
substitutions. Due to the absence of cyano groups it provides high signal-to-noise ratios for ECD
detectors. The 100 m column separates critical isomer pairs: 28/31, 56/60, 149/118,
105/153/132 and 170/190.

CP-Sil 5/C18 CB for PCB
ID (mm)
0.25
0.32

Length (m)
50
100
100

Film (µm)
0.10
0.10
0.10

Temp Limits (°C)
275/300
275/300
275/300

7 in. Cage
CP7477
CP7476
CP7478

AGILENT J&W GC AND GC/MS COLUMNS

507

Semivolatiles Columns

CP-Sil 8 CB for PCB
• Guaranteed for the analysis of PCBs according to DIN Method 51527
for confidence in results

• Suitable for high sensitivity ECD detection for low detection limits
• High temperature stability provides extended lifetime and enhanced productivity
CP-Sil 8 CB has high temperature stability and is resistant to continuous splitless injections.
Due to the absence of cyano groups the column provides high signal-to-noise ratios on ECD.

CP-Sil 8 CB for PCB
ID (mm)
0.25

Length (m)
50

Film (µm)
0.25

Temp Limits (°C)
300/325

7 in. Cage
CP7482

Film (µm)
0.25

Temp Limits (°C)
300/325

7 in. Cage
CP7482

Select 28/31
Select 28/31
ID (mm)
0.25

508

GC AND GC/MS

Length (m)
50

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Volatiles Columns

Volatiles Columns
Recommended Columns for Volatiles
• DB-VRX and DB-624 for EPA Methods 502.2 and 8021
• DB-VRX (60 m, 0.25 mm ID) for GC/MS volatiles methods
• DB-VRX (20 m, 0.18 mm ID) for fast GC/MS volatiles analysis using a 5973 MSD
(Not recommended for Ion Trap MS or older MSDs)

• DB-MTBE for extended EPA Method 8020
• DB-TPH for the analysis of BTEX and gasoline total petroleum hydrocarbons
• DB-624 (20 m, 0.18 mm ID) for fast GC/MS volatiles analysis
Agilent offers a selection of advanced polymer chemistries for increasingly demanding volatiles
applications. Whether for a primary analytical column or as a complementary confirmation
column, Agilent J&W capillaries are chromatographers' first choice.

Tips & Tools
Find all the tools you need for column installation in
Agilent's Column Installation Kit, P/N 430-2000.

AGILENT J&W GC AND GC/MS COLUMNS

509

Volatiles Columns

DB-VRX
• Unique selectivity engineered for optimum resolution of volatiles analysis:
U.S. EPA Methods 502.2, 524.2 and 8260

• 0.45 mm ID columns provide more plates per meter compared to 0.53 mm ID columns
for the fewest coelutions for GC method (an industry first)*

• No subambient cooling required to resolve the six "gases"
• Fast run time:
<30 minutes for optimum sample throughput
<8 minutes with 0.18 mm ID
• Low polarity
• Excellent peak shape
• Bonded and cross-linked
• Solvent rinsable

*Two coelutions: 1) m- and p-xylene, for which U.S. EPA does not require separation,
and 2) 1,1,2,2-tetrachloroethane and o-xylene which are separated by detectors PID
and ELCD, respectively. Note to GC/MS analysts: These coeluting compounds have
different primary characteristic ions of 83 and 106, respectively.

DB-VRX Chromatograms
Environmental
EPA Volatiles by GC/MS (Split Injector)
High Speed VOC, EPA Method 8260
Unleaded Gasoline
Extended Analyte List for EPA Method 8021

Page 604
Page 606
Page 569
Page 607

DB-VRX
ID (mm)
0.18
0.25
0.32
0.45

510

Length (m)
20
40
30
60
30
60
30
75

GC AND GC/MS

Film (µm)
1.00
1.00
1.40
1.40
1.80
1.80
2.55
2.55

Temp Limits (°C)
-10 to 260
-10 to 260
-10 to 260
-10 to 260
-10 to 260
-10 to 260
-10 to 260
-10 to 260

7 in. Cage
121-1524
121-1544
122-1534
122-1564
123-1534
123-1564
124-1534
124-1574

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

5 in. Cage
121-1544E

7890/6890
LTM Module
121-1524LTM
121-1544LTM
122-1534LTM

122-1564E
123-1534LTM
123-1564E
124-1534LTM

5975T
LTM Toroid
221-1524LTM
222-1534LTM

Volatiles Columns

DB-624
• Specifically designed for the analysis of volatile priority pollutants and residual solvents
• No cryogenics needed for U.S. EPA Method 502.2
• Excellent for U.S. EPA Methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020,
8240, 8260, and USP 467

• Excellent inertness for active compounds
• Bonded and cross-linked
• Solvent rinsable
• Exact replacement of HP-624
• Equivalent to USP Phase G43
DB-624 Chromatograms
Environmental
EPA Volatiles by GC/MS II (Split Injector)
European Red List Volatiles
Extended Analyte List for EPA Method 8021
Fast VOC Analysis

Page 605
Page 609
Page 607
Page 608

Food, Flavors and Fragrances
Fusel Oil Standard & Brandy Sample

Page 619

Industrial Chemicals
Alcohols I
Esters II
Ethers
Glycols II
Halogenated Hydrocarbons I
Nitrogen Based Solvents II

Page 641
Page 653
Page 654
Page 655
Page 658
Page 663

Life Sciences
Residual Solvents, DMI Diluent
Residual Solvents, USP 467

Page 684
Page 684

Petroleum
1,3-Butadiene

Page 690

AGILENT J&W GC AND GC/MS COLUMNS

511

Volatiles Columns

DB-624
ID (mm)
0.18
0.20
0.25
0.32
0.45
0.53

Length (m)
20
25
30
60
30
60
30
75
15
30
60
75

Film (µm)
1.00
1.12
1.40
1.40
1.80
1.80
2.55
2.55
3.00
3.00
3.00
3.00

Temp Limits (°C)
-20 to 280
-20 to 260
-20 to 260
-20 to 260
-20 to 260
-20 to 260
-20 to 260
-20 to 260
-20 to 260
-20 to 260
-20 to 260
-20 to 260

7 in. Cage
121-1324
128-1324
122-1334
122-1364
123-1334
123-1364
124-1334
124-1374
125-1314
125-1334
125-1364
125-1374

5 in. Cage
121-1324E
128-1324E
122-1334E
122-1364E
123-1334E
123-1364E

7890/6890
LTM Module
121-1324LTM
128-1324LTM
122-1334LTM

5975T
LTM Toroid
221-1324LTM
222-1334LTM

123-1334LTM
124-1334LTM

125-1334E
125-1364E
125-1374E

125-1334LTM

CP-Select 624 CB
• Guaranteed for EPA volatiles with methods 524.2, 624 and 8015 for maximum confidence
• Excellent peak shape for polar and basic compounds for accurate results
• Specified by Pharmacopoeia Method V.3.3.9 for residual solvent analysis,
providing compliancy

The CP-Select 624 CB is a highly reproducible version of the popular 624 phase and has 2-3
times lower bleed (bleed specification for a 30 m, 0.53 mm, df = 3.00 µm is <9 pA) than
conventional columns. The Select 624 CB, a 6% cyanopropylphenyl, 94% dimethylsiloxane
phase is synthesized and fully characterized to ensure maximum column-to-column
reproducibility. For an even lower bleed performance we recommend the VF-624ms.

CP-Select 624 CB
ID (mm)
0.15
0.25
0.32
0.53

512

GC AND GC/MS

Length (m)
25
30
60
30
60
30
75
105

Film (µm)
0.84
1.40
1.40
1.80
1.80
3.00
3.00
3.00

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
265/280
265/280
265/280
265/280
265/280
265/280
265/280
265/280

7 in. Cage
CP7411
CP7412
CP7413
CP7414
CP7415
CP7416
CP7417
CP7418

5 in. Cage
CP7411I5
CP7413I5
CP7414I5
CP7415I5
CP7416I5

Volatiles Columns

HP-VOC
• Selectivity engineered for U.S. EPA Methods 502.2, 524.2 and 8260
• Low polarity – slightly more polar than DB-VRX
• Excellent peak shape
• Bonded and cross-linked
• Solvent rinsable
HP-VOC
ID (mm)
0.20
0.32
0.53

Length (m)
30
60
60
90
90
105

Film (µm)
1.10
1.10
1.80
1.80
3.00
3.00

Temp Limits (°C)
-60 to 280/290
-60 to 280/290
-60 to 280/290
-60 to 280/290
-60 to 280/290
-60 to 280/290

7 in. Cage
19091R-303
19091R-306
19091R-316
19091R-319
19095R-429
19095R-420

7890/6890
LTM Module
19091R-303LTM

5975T
LTM Toroid
29091R-303LTM

DB-502.2
• Available in 105 m for volatiles analyses
• Excellent peak shape
• Bonded and cross-linked
• Solvent rinsable
DB-502.2
ID (mm)
0.25
0.32
0.45
0.53

Length (m)
60
60
75
105
105

Film (µm)
1.40
1.80
2.55
2.55
3.00

Temp Limits (°C)
0 to 260/280
0 to 260/280
0 to 260/280
0 to 260/280
0 to 260/280

7 in. Cage
122-1464
123-1464
124-1474
124-14a4
124-14A4

AGILENT J&W GC AND GC/MS COLUMNS

513

Volatiles Columns

DB-MTBE
• Low polarity stationary phase
• Resolves MTBE from 2-methylpentane and 3-methylpentane for better quantitation
• Engineered for purge and trap injection without the need for cryofocusing
• Bonded and cross-linked
• Solvent rinsable
DB-MTBE Chromatograms
Environmental
Methyl Tert-Butyl Ether (MTBE) FID, Extended 8020 Analysis

Page 569

DB-MTBE
ID
(mm)
0.45
0.53

Length
(m)
30
30

Film
(µm)
2.55
3.00

Temp Limits (°C)
35 to 260/280
35 to 260/280

7 in. Cage
125-14A4
125-0034

5 in. Cage
125-0034E

7890/6890
LTM Module
124-0034LTM
125-0034LTM

CP-Select CB for MTBE
• Guaranteed analysis of MTBE in reformulated gasolines for reproducible results
• Unique selectivity for ease-of-use with MTBE
• Broad dynamic range for quantification of MTBE for the highest productivity
CP-Select CB for MTBE
ID (mm)
0.25

514

GC AND GC/MS

Length (m)
50

Film (µm)
0.25

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
200/200

7 in. Cage
CP7528

Volatiles Columns

DB-TPH
• Specifically designed for the analysis of total petroleum hydrocarbons (TPHs), soil analysis,
and LUFT

• Three analyses in one injection – gas range organics, diesel range organics and motor oil
• Fast run time
• Bonded and cross-linked
• Solvent rinsable
DB-TPH
ID (mm)
0.32
0.45

Length (m)
30
30

Film (µm)
0.25
1.00

Temp Limits (°C)
-10 to 320
-10 to 290

7 in. Cage
123-1632
124-1632

7890/6890
LTM Module
123-1632LTM

Tips & Tools
For a precision cut on your capillary column, use Agilent's
GC column cutting tool (P/N 5183-4620).

AGILENT J&W GC AND GC/MS COLUMNS

515

Volatiles Columns

Select Mineral Oil
• Optimized selectivity for more reliable results
• Low bleed for better accuracy
• Available in UltiMetal for ultimate longevity
Total petroleum hydrocarbon (TPH) analysis is a routine technique used in environmental
laboratories screening many samples. A simple and reliable method that provides the shortest
analysis time is required. Select Mineral Oil is designed to meet this need, with a stabilized,
non-polar bonded phase specifically for fast mineral oil analysis. The column is temperature
stable up to 375/400°C and provides speedy analyses according to DIN H53 and DIN-EN-ISO
9377-2 methods. Thanks to the temperature stability of Select Mineral Oil, your C4 to C40
hydrocarbons can be analyzed in less then ten minutes. The high temperature stability
of the column permits faster bakeout. For optimal injection performance be sure to use the
special 4 m retention gap. Select Mineral Oils are available in economical three- and six-packs.

Select Mineral Oil
ID (mm)
0.32

Length (m)
15
15
15

Film (µm)
0.10
0.10
0.10

Temp Limits (°C)
-60 to 390/400
-60 to 390/400
-60 to 390/400

Unit
1/pk
3/pk
6/pk

7 in. Cage
CP7491
CP749103
CP749106

-60 to 325/350

3/pk

CP8015

5 in. Cage
CP7491I5

Retention gap

0.53

4

Select Mineral Oil UltiMetal
ID (mm)
0.32

516

GC AND GC/MS

Length (m)
15

Film (µm)
0.10

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
-60 to 390/400

7 in. Cage
CP7493

Metal Columns

Metal Columns
Recommended Metal Columns
• CP-Sil 13 CB UltiMetal
• CP-Sil 5 CB UltiMetal
• CP-SIL 8 CB UltiMetal
• CP-WAX 52 CB UltiMetal
• FactorFour VF-5HT UltiMetal
• CP-AL2O3/KCL UltiMetal
• CP-AL2O3/NA2SO4 UltiMetal
• CP-PoraPLOT Q UltiMetal
• PLOT Molesieve 5Å UltiMetal

DB-ProSteel
• Excellent inertness
• Virtually unbreakable
• Available in a wide variety of stationary phases
• Bonded and cross-linked
• Ideal for high temperature analysis and process applications
Our easy-to-handle DB-ProSteel metal columns are deactivated with a new formula (this is not
glass lined steel) to provide inertness that truly rivals fused silica. DB-ProSteel metal columns can
be custom wound upon request for small GC ovens. Several of our most popular bonded phases
are available in metal.
DB-ProSteel GC columns have the same outer diameter as standard Megabore (0.53 mm ID),
so no special ferrules are required.

AGILENT J&W GC AND GC/MS COLUMNS

517

Metal Columns

DB-ProSteel
Phase
DB-PS1

ID (mm)
0.53

Length (m)
15

30

DB-PS624
DB-PSWAX
DB-PS2887

0.53
0.53
0.53

30
30
10

Film (µm)
0.15
0.50
1.50
0.15
0.50
3.00
5.00
3.00
1.00
3.00

Tips & Tools
For a comprehensive listing of chromatograms searchable
by compound name, visit our online Chromatogram Library
at www.agilent.com/chem/library

518

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Temp Limits (°C)
-60 to 340/360
-60 to 300/320
-60 to 300/320
-60 to 340/360
-60 to 300/320
-60 to 260/280
-60 to 260/280
-20 to 260
20 to 230/240
-60 to 350

7 in. Cage
145-1011
145-1017
145-1012
145-1031
145-1037
145-1034
145-1035
145-1334
145-7032
145-2814

Non-Bonded Stationary Phases

Non-Bonded Stationary Phases
Whenever possible Agilent recommends the use of bonded and cross-linked polymers.
Bonded polymers are more rugged, will have longer lifetimes and can be solvent rinsed.
However, Agilent recognizes that some methods have been developed on non-bonded
phases and therefore maintains these columns to support established methods.

HP-101
• 100% Dimethylpolysiloxane
Because HP-101 columns are not bonded or cross-linked, we do not recommend
solvent rinsing.

HP-101
ID (mm)
0.20

0.32

Length (m)
10
25
50
12
25
50

Film (µm)
0.20
0.20
0.20
0.25
0.30
0.30

Temp Limits (°C)
-60 to 280
-60 to 280
-60 to 280
-60 to 280
-60 to 280
-60 to 280

7890/6890
7 in. Cage
5 in. Cage
LTM Module
19091Y-101
19091Y-102
19091Y-102LTM
19091Y-105
19091-60010 19091-60010E
19091Y-012 19091Y-012E 19091Y-012LTM
19091Y-015

AGILENT J&W GC AND GC/MS COLUMNS

519

Non-Bonded Stationary Phases

HP-17
• 50% Phenyl and 50% Methyl siloxane
Because the HP-17 is not bonded or cross-linked, we do not recommend solvent rinsing.

HP-17
ID (mm)
0.53

Length (m) Film (µm)
10
2.00

Temp Limits (°C)
25 to 260/280

7 in. Cage
19095L-121

7890/6890
LTM Module
19095L-121LTM

CAM
• Base deactivated polyethylene glycol
• Specifically designed for amine analysis
• Excellent peak shape for primary amines
• Replaces HP-Basicwax
Because the CAM is not bonded or cross-linked, we do not recommend solvent rinsing.

CAM Chromatograms
Industrial Chemicals
Amines in Water
Primary Amines

Page 647
Page 645

CAM
ID (mm)
0.25

520

Length (m)
15
30

0.32

60
30

0.53

30

GC AND GC/MS

Film (µm)
0.25
0.25
0.50
0.25
0.25
0.50
1.00

Temp Limits (°C)
60 to 220/240
60 to 220/240
60 to 220/240
60 to 220/240
60 to 220/240
60 to 220/240
60 to 200/220

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7 in. Cage
112-2112
112-2132
112-2133
112-2162
113-2132
113-2133
115-2132

5 in. Cage

113-2132E
113-2133E

7890/6890
LTM Module
112-2112LTM
112-2132LTM
112-2133LTM
113-2132LTM
113-2133LTM
115-2132LTM

Non-Bonded Stationary Phases

Carbowax 20M and HP-20M
• Polyethylene glycol, MW 20,000
• Equivalent to USP Phase G16
Because the Carbowax 20M and the HP-20M are not bonded or cross-linked, we do not
recommend solvent rinsing. DB-WAX is the recommended bonded alternate for the HP-20M.

Carbowax 20M
ID (mm)
0.25
0.32

Length (m)
30
30
60

Film (µm)
0.25
0.25
0.25

Temp Limits (°C)
60 to 220/240
60 to 220/240
60 to 220/240

7 in. Cage
112-2032
113-2032
113-2062

7890/6890
LTM Module
112-2032LTM
113-2032LTM

HP-20M
ID (mm)
0.20
0.32
0.53

Length (m)
25
50
25
50
10
30

Film (µm)
0.10
0.10
0.30
0.30
1.33
1.33

Temp Limits (°C)
60 to 220
60 to 220
60 to 220
60 to 220
60 to 220
60 to 220

7 in. Cage
5 in. Cage
19091W-102
19091W-105
19091W-012 19091W-012E
19091W-015 19091W-015E
19095W-121
19095W-123

7890/6890
LTM Module
19091W-102LTM
19091W-012LTM
19095W-121LTM
19095W-123LTM

AGILENT J&W GC AND GC/MS COLUMNS

521

Non-Bonded Stationary Phases

DX-1 and DX-4
• DX-1: 90% Dimethylpolysiloxane 10% Polyethylene Glycol
• DX-4: 15% Dimethylpolysiloxane 85% Polyethylene Glycol
Because DX series GC columns are not bonded and cross-linked, we do not recommend
solvent rinsing.

DX-1
ID (mm)
0.25
0.32

Length (m)
30
30

Film (µm)
1.00
1.00

Temp Limits (°C)
50 to 250/270
50 to 250/270

7 in. Cage
122-6133
123-6133

Length (m)
30
60
15
30
60

Film (µm)
0.25
0.25
0.25
0.25
0.25

Temp Limits (°C)
50 to 250/270
50 to 250/270
50 to 250/270
50 to 250/270
50 to 250/270

7 in. Cage
122-6432
122-6462
123-6412
123-6432
123-6462

7890/6890
LTM Module
123-6133LTM

DX-4
ID (mm)
0.25
0.32

522

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

7890/6890
LTM Module
122-6432LTM
123-6412LTM
123-6432LTM

Non-Bonded Stationary Phases

SE-30 and SE-54
• SE-30: 100% Dimethylpolysiloxane
• SE-54: (5%-Phenyl)(1%-Vinyl)-methylpolysiloxane
Because SE series GC columns are not bonded or cross-linked, we do not recommend
solvent rinsing.

SE-30
ID (mm)
0.32

Length (m)
30

Film (µm)
0.25

Temp Limits (°C)
0 to 325/350

7 in. Cage
113-3032

Length (m)
30
60
30

Film (µm)
0.25
0.25
0.25

Temp Limits (°C)
0 to 325/350
0 to 325/350
0 to 325/350

7 in. Cage
112-5432
112-5462
113-5432

7890/6890
LTM Module
113-3032LTM

SE-54
ID (mm)
0.25
0.32

7890/6890
LTM Module
112-5432LTM
113-5432LTM

AGILENT J&W GC AND GC/MS COLUMNS

523

Packed GC Columns

Packed GC Columns
Stationary phases are available for the production of packed columns and coated packings.

Stationary Phases for Packed GC Columns

524

A
Antarox CO 630
Antarox CO 880
Antarox CO 990
Apiezon H
Apiezon J
Apiezon K
Apiezon L
Apiezon M
Apiezon N
Armeen SD
B
Bentone 34
Benzylcyanide-silver nitrate
Bis(2-butoxyetylphthalate)
Benzylpyridine

C
CP-Sil 34
CP-Sil 5
CP-Sil 58
CP-Sil 76
CP-Sil 8
CP-Sil 84
CP-Sil 88
CP-Wax 4000 M
CP-Wax 600 M
Cyano B

Bis(2-ethoxyethyladipate)
Bis(2-cyanoethyl)formamide N,NBis(2-ethoxyethylsebacate)
Bis(2-methoxyethyl)adipate
Butanediol succinate
Bis(p-butoxybenziline)-α,α-bi-p-toluidine N,N-

Decaglycerol
Decane nDexsil 300 GC polymethylcarborane
Dexsil 400 GC polymethylcarborane
Dexsil 410 GC polymethylcarborane
Di isodecyl-phthalate, DIDP

Bis(p-methoxybenzilidine)-α,α-bi-p-toluidine N,N-

Di isooctyl-adipate

C
Carbowax 1000
Carbowax 10000
Carbowax 1500
Carbowax 1540
Carbowax 200
Carbowax 20M
Carbowax 20M TPA
Carbowax 300
Carbowax 400
Carbowax 4000
Carbowax 4000 TPA
Carbowax 550
Carbowax 600
Carbowax 6000
Carbowax 750
Castorwax
Celanese ester

Di isooctyl-phthalate
Di isooctyl-sebacate, DEHS
Di-n-decyl phthalate, DDP
Di-n-octyl adipate
Di-n-propyl phthalate
Di-n-propyl tetrachlorophthalate
Dioctoil
Dioctyl phthalate
Dioctyl sebacate (Octoil s)
Dibenzyl ether
Dibutyl maleate
Dibutyl phthalate, DBP
Dibutyl tetrachlorophthalate, DBTP
Diethylene glycol
Diethylene glycol adipate, DEGA, cross linked
Diethylene glycol adipate, DEGA
Diethylene glycol sebacate, DEGSe
Diethylene glycol succinate, DEGS

GC AND GC/MS

Cyanoethyl sucrose
Cyanoethyl sucrose, CES
Cyclo N
Cyclohexane dimethanol succinate, CDS
D

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Packed GC Columns

Stationary Phases for Packed GC Columns
D
Diglycerol
Dimer acid
Dimethylformamide, DMF
Dimethyl sulfolane, DMS 2,4Dimethanol cyclohexane succinate, CDS
Dimethyl sulfoxide, DMSO
Dinonyl phthalate
Dinonyl sebacate
DOW corning 705
Dow fax 9 N 40
Dowfax 9N9

H
Hexadecene
Hexadecanol
Hexakis(2-cyanoethoxy)cyclohexane 1,2,3,4,5,6Hexamethyl phosphoramide, HMPA
HI-EFF 1 AP
HI-EFF 1 BP
HI-EFF 3 AP
HI-EFF 3 B
HI-EFF 8 BP
Hyprose SP-80, (octakis-(2-hydroxypropyl)sucrose)
I

E
EGSS-X
Emulphor ON-870
Epon 1001, epoxy resin
Ethofat 60/25
Ethylbenzene
Ethylene glycol adipate, EGA
Ethylene glycol isophthalate
Ethylene glycol isophthalate EGIP
Ethylene glycol phthalate
Ethylene glycol sebacate
Ethylene glycol succinate
Ethylene glycol tetrachlorophthalate
F

Isoquinoline
K

FFAP
Fluorad FC 431
Fluorene
Fluorolube GR-362
Fyrquel 220
G
Glycerol
H
H3PO4 (Phosphoric acid)
Hallcomid M-18, dimethylstearamide
Hallcomid M-18-OL, dimethyloleamide
Halocarbon K-352
Halocarbon oil 10.25
Halocarbon wax
Hexadecane

Kel F grease
Kel F oil no. 10
Kel F oil no. 3
Kel F wax
KOH (potassium hydroxide)
L
LAC 1 R 296
LAC 10 R 744
LAC 12 R 796
LAC 17-R-770
LAC 22 R 863
Lexan (polycarbonate resin)
M
Mannitol
Montan wax
N
Neopentyl glycol adipate
Neopentyl glycol sebacate
Neopentyl glycol succinate
Nitrobenzene
Nujol (paraffin oil)
O
Octadecane nOctadecene nOctoil
Olive oil
Oronite NIW

AGILENT J&W GC AND GC/MS COLUMNS

525

Packed GC Columns

Stationary Phases for Packed GC Columns
O
Oronite polybutene 128
Oronite polybutene 32
OS-124 (PMPE 5 ring)
OS-138 (PMPE 6 ring)
P
Palladium
Paraffin oil
Paraffin wax
Pentanediolsuccinate; 1,5Phenyl diethanolamine
Phenyl diethanolamine succinate
Pluronic P84
Poly-A 101A (polyamide)
Poly-A 103 (polyamide)
Poly-A 135 (polyamide)
Poly-L 110 (polyamide)
Poly-m-phenyl ether 5 ring
Poly-m-phenoxylene, PPE-21
Poly-m-phenyl ether 6 ring
Poly-S 179
Polyethylene glycol 2000
Polyethylene glycol 600, Jefferson
Polyethylene imine
Polypropylene glycol 2000
Polypropylene glycol 3500
Polypropylene glycol 4000
Polypropylene imine
Polyvinylpyrrolidone
Q
Quadrol
R
Reoplex 4000
S
Sebaconitrile
Silar 10 C
Silar 5 CP highly polar
Silar 7 C
Silar 9 C
Silicone AN-600 (50% cyanoethyl)
Silicone DC 111 grease

526

GC AND GC/MS

S
Silicone DC 200
Silicone DC 410
Silicone DC 550
Silicone DC 560
Silicone DC 702
Silicone DC-704
Silicone DC 710
Silicone fluid MS 550
Silicone OV-1
Silicone OV-101
Silicone OV-105
Silicone OV-11
Silicone OV-17
Silicone OV-1701
Silicone OV-202
Silicone OV-210
Silicone OV-215
Silicone OV-22
Silicone OV-225
Silicone OV-25
Silicone OV-275
Silicone OV-3
Silicone OV-330
Silicone OV-351
Silicone OV-61
Silicone OV-7
Silicone OV-73
Silicone QF-1
Silicone SE-30
Silicone SE-30 GC Grade
Silicone SE-52
Silicone SE-54
Silicone SF-96
Silicone UC W-98
Silicone UC W-982
Silver nitrate
Sorbitol
SP-1000
SP-1200
SP-2100

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Packed GC Columns

Stationary Phases for Packed GC Columns
S
SP-2250
SP-2300
SP-2310
SP-2330
SP-2340
SP-2401
SP-300
Span-80
Squalane
Squalene
Sucrose acetate isobutyrate
Sucrose-octa acetate
Supelco SP-216 PS
Surfonic N-300
T
Terephthalic acid
Tergitol NP-35
Tergitol NPX
Tetra ethylene glycol
Tetracyanoethyl pentaerythritol
Tetraethylene glycol dimethyl ether
Tetraethylene pentamine
Tetrahydroxyethylenediamine
Thiodipropionitrile β,β-

U
UC-L-45
UCON 50 HB 2000
UCON 50 HB 280X
UCON 50 HB 5100
UCON 75 H 90000
UCON LB 1200X
UCON LB 1715
UCON LB 1800X
UCON LB 550X
UCW-98
UC W-982
V
Versamid 900 (polyamide resin)

Tri(tetra hydrofuryl)phosphate
Triacetin
Tributylphosphate
Triethanolamine
Trimer acid
Trimethylol pelargonate
Triton X-100
Tris(2-cyanoethoxy)propane 1,2,3Tritolyl phosphate
Triton X-305
Tween-80

AGILENT J&W GC AND GC/MS COLUMNS

527

Packed GC Columns

Supports for Packed GC Columns
Supports are available for the production of packed columns and coated packings.

Supports for Packed GC Columns
Description
Activated charcoal
Activated charcoal
Activated charcoal
Alumina GC
Alumina GC
Alumina GC
Carbopack B
Carbopack B
Carbopack C
Carbopack C
Carbosieve G
Carbosieve G
Carbosieve S II
Carbosieve S II
Carbosieve S III
Carbosieve S III
Carbosphere
Carbosphere
Chromosorb 101
Chromosorb 101
Chromosorb 101
Chromosorb 102
Chromosorb 102
Chromosorb 102
Chromosorb 102
Chromosorb 103
Chromosorb 103
Chromosorb 105
Chromosorb 105
Chromosorb 106
Chromosorb 106
Chromosorb 106
Chromosorb 107
Chromosorb 107
Chromosorb 108
Chromosorb 108
Chromosorb 750
Chromosorb G AW
Chromosorb G AW
Chromosorb G AW
Chromosorb G AW DMCS

528

GC AND GC/MS

Mesh Size
40-60
60-80
80-100
40-60
60-80
80-100
60-80
80-100
60-80
80-100
60-80
80-100
60-80
80-100
60-80
80-100
60-80
80-100
60-80
80-100
100-120
20-40
60-80
80-100
100-120
80-100
100-120
80-100
100-120
60-80
80-100
100-120
80-100
100-120
80-100
100-120
80-100
60-80
80-100
100-120
60-80

Description
Chromosorb G AW DMCS
Chromosorb G AW DMCS
Chromosorb G HP
Chromosorb G HP
Chromosorb G HP
Chromosorb G HP
Chromosorb G NAW
Chromosorb G NAW
Chromosorb G NAW
Chromosorb G NAW
Chromosorb P
Chromosorb P
Chromosorb P
Chromosorb P
Chromosorb P
Chromosorb P AW
Chromosorb P AW
Chromosorb P AW
Chromosorb P AW
Chromosorb P AW DMCS
Chromosorb P AW DMCS
Chromosorb P AW DMCS
Chromosorb P AW DMCS
Chromosorb P NAW
Chromosorb P NAW
Chromosorb P NAW
Chromosorb P NAW
Chromosorb T
Chromosorb T
Chromosorb W AW
Chromosorb W AW
Chromosorb W AW
Chromosorb W AW
Chromosorb W AW DMCS
Chromosorb W AW DMCS
Chromosorb W AW DMCS
Chromosorb W AW DMCS
Chromosorb W HMDS
Chromosorb W HMDS
Chromosorb W HMDS
Chromosorb W HMDS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Mesh Size
80-100
100-120
45-60
60-80
80-100
100-120
45-60
60-80
80-100
100-120
20-40
40-60
60-80
80-100
100-120
45-60
60-80
80-100
100-120
45-60
60-80
80-100
100-120
45-60
60-80
80-100
100-120
30-60
40-60
40-60
60-80
80-100
100-120
45-60
60-80
80-100
100-120
45-60
60-80
80-100
100-120

Packed GC Columns

Supports for Packed GC Columns
Description
Chromosorb W HP
Chromosorb W HP
Chromosorb W HP
Chromosorb W NAW
Chromosorb W NAW
Chromosorb W NAW
Glass beads regular
Glass beads regular
Glass beads regular
Glass beads regular
Hayesep A
Hayesep A
Hayesep A
Hayesep B
Hayesep B
Hayesep B
Hayesep C
Hayesep C
Hayesep C
Hayesep N
Hayesep N
Hayesep N
Hayesep P
Hayesep P
Hayesep P
Hayesep Q
Hayesep Q
Hayesep Q
Hayesep R
Hayesep R
Hayesep R
Hayesep S
Hayesep S
Hayesep S
Hayesep T
Hayesep T
Hayesep T
Molecular sieve 5Å
Molecular sieve 5Å
Molecular sieve 5Å
Molecular sieve 5Å
Molecular sieve 13X
Molecular sieve 13X
Molecular sieve 13X

Mesh Size
60-80
80-100
100-120
60-80
80-100
100-120
45-60
60-80
80-100
100-120
60-80
80-100
100-120
60-80
80-100
100-120
60-80
80-100
100-120
60-80
80-100
100-120
60-80
80-100
100-120
60-80
80-100
100-120
60-80
80-100
100-120
60-80
80-100
100-120
50-80
80-100
100-120
45-60
60-80
80-100
100-120
60-80
80-100
100-120

Description
Porapak N
Porapak N
Porapak N
Porapak P
Porapak P
Porapak P
Porapak PS
Porapak PS
Porapak Q
Porapak Q
Porapak Q
Porapak QS
Porapak QS
Porapak QS
Porapak R
Porapak R
Porapak R
Porapak S
Porapak S
Porapak S
Porapak T
Porapak T
Porapak T
Porasil B
Porasil C
Silica gel GC grade
Silica gel GC grade
Silica gel GC grade
Silocel
Silocel
Silocel
Silocel
Spherosil XOB 75
Tenax GR
Tenax GR
Tenax GR
Tenax TA
Tenax TA
Tenax TA
Tenax TA
W KOH washed
W KOH washed
W KOH washed
W KOH washed

Mesh Size
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
50-80
80-100
100-120
80-100
80-100
30-40
45-60
60-80
45-60
60-80
80-100
100-120
100-120
35-60
60-80
80-100
20-35
35-60
60-80
80-100
45-60
60-80
80-100
100-120

AGILENT J&W GC AND GC/MS COLUMNS

529

Custom GC Columns

Custom GC Column Ordering
Even though we offer over a thousand readily available columns, Agilent recognizes that
sometimes you need something a little out of the ordinary. That's why we developed our Custom
Column Shop. If you can't find what you're looking for in our standard order guides, we will
design, build, and test capillary GC columns to meet your needs.

• We can create columns with non-standard lengths or unusual film thickness.
• We can connect columns together in series or as dual columns.
• We recognize that sometimes customers have specific column performance requirements for
their applications that might not be met with standard test mixes. As a result we can also
custom-test your columns with your desired test mixture and test conditions to meet your
specific performance requirements.

• We can create DuraGuard columns with an integrated guard column. Most phases can be

manufactured with a built-in guard column which means you get the advantages of a guard
column without the union. Only available in DB-phases.

Custom columns are ordered using P/N 100-2000. Be sure to provide the details of your desired
custom service or column including phase, length, ID, and film thickness.
Contact your local Agilent office or Authorized Agilent Distributor to receive a quote for your
custom column needs. You can find order forms in the back of the catalog.
Customers in the United States, Canada, and Puerto Rico can request a custom column quote
online at www.agilent.com/chem/CustomColumn

530

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Agilent J&W GC Column Test Standards

Tech Support
Need assistance selecting a column
for your method? Contact our
chromatography technical specialists at
www.agilent.com/chem/TechRep

Agilent J&W GC Column Test Standards
Compare your column's performance to the test chromatogram shipped with your J&W column
from Agilent. The column test standard contains components that test the column for resolution
characteristics, efficiency, and inertness. The test mixes are supplied at a concentration of 250
ng/µL in 2 mL vials. Match the phase and column diameter in the chart below to find the test mix
for your column.

Agilent J&W GC Column Test Standards
Column
Description
OV-351
DB-1ht
DB-1
DB-5
DB-5ht
DB-5ms
DB-624
DB-2887
DB-WAX
DB-WAXetr
SE-30
SE-52
SE-54
HP-1
HP-5
HP-FFAP
GS-OxyPLOT

Microbore
(0.05 & 0.10 mm ID)
Part No.

200-0010
200-0010

200-0070

8500-6813

Capillary
(0.18 & 0.32 mm ID)
Part No.
200-0032
200-0010
200-0310
200-0310
200-0010
200-0185
200-0113
200-0370
200-0370
200-0010
200-0010
200-0010
5080-8858
5080-8858
8500-6813

Megabore
(0.45 & 0.53 mm ID)
Part No.

200-0110
200-0110
200-0185
200-0113
200-0110
200-0070
200-0070

200-0010
8500-6812
8500-6812
8500-6813
5188-5379

AGILENT J&W GC AND GC/MS COLUMNS

531

Fused Silica Tubing

Fused Silica Tubing
Deactivated Tubing
Deactivated tubing can be used as retention gaps, guard columns, or transfer lines. Our standard
deactivation process is a phenyl methyl deactivation – the preferred choice for most applications
due to its inertness and robustness.

Deactivated Fused Silica
ID (mm)
0.05

OD (mm)
0.36

0.10

0.19

0.36

532

GC AND GC/MS

0.15

0.36

0.18

0.34

0.20

0.36

0.25

0.36

0.32

0.43

0.45

0.67

0.53

0.67

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

Length (m)
1
5
10
1
5
10
1
5
5
10
1
5
10
1
5
10
1
5
10
1
5
10
30
1
5
10
30
1
5
10
1
5
10
30

Part No.
160-2655-1
160-2655-5
160-2655-10
160-1010-1
160-1010-5
160-1010-10
160-2635-1
160-2635-5
19091-60620E
160-2635-10
160-2625-1
160-2625-5
160-2625-10
160-2615-1
160-2615-5
160-2615-10
160-2205-1
160-2205-5
160-2205-10
160-2255-1
160-2255-5
160-2255-10
160-2255-30
160-2325-1
160-2325-5
160-2325-10
160-2325-30
160-2455-1
160-2455-5
160-2455-10
160-2535-1
160-2535-5
160-2535-10
160-2535-30

Fused Silica Tubing

Deactivated Fused Silica High Temperature (400°C)
ID (mm)
0.05
0.10
0.25

OD (mm)
0.36
0.36
0.35

0.32

0.43

0.53

0.67

Length (m)
5
5
5
10
5
10
5
10

Part No.
160-2815-5
160-2825-5
160-2845-5
160-2845-10
160-2855-5
160-2855-10
160-2865-5
160-2865-10

ProSteel Deactivated Fused Silica
ID (mm)
0.53

OD (mm)
0.67

Length (m)
5

Part No.
160-4535-5

Undeactivated Fused Silica
Undeactivated tubing or bare fused silica is commonly used for capillary electrophoresis. It can
also be used for transfer lines and other applications where inertness is not critical.

Undeactivated Fused Silica
ID (mm)
0.02
0.05

OD (mm)
0.36
0.36

0.075

0.36

0.10

0.36

0.18

0.34

0.20

0.36

0.25

0.36

0.32

0.43

0.45

0.67

0.53

0.67

Length (m)
5
5
10
5
10
5
10
5
10
5
10
50
5
10
5
10
50
5
10
5
10

Part No.
160-2660-5
160-2650-5
160-2650-10
160-2644-5
160-2644-10
160-2634-5
160-2634-10
160-2610-5
160-2610-10
160-2200-5
160-2200-10
19091-20050
160-2250-5
160-2250-10
160-2320-5
160-2320-10
19091-21050
160-2450-5
160-2450-10
160-2530-5
160-2530-10

AGILENT J&W GC AND GC/MS COLUMNS

533

GC Column Application and Method Guides

GC Column Application and Method Guides
Application

Specific Application

Agilent Phase

Biodiesel

EN14105 Free/Total Glycerin
ASTM D6584 Free/Total Glycerin
EN14103 FAME Analysis
EN14110 Residual Methanol
EN14106 Free Glycerol
Chiral γ-lactones and terpenes

Biodiesel, Select Biodiesel
Biodiesel, Select Biodiesel
Biodiesel, Select Biodiesel
Biodiesel, Select Biodiesel
Select Biodiesel
CycloSil-B

Optical isomers of acids, alcohols, amino acids,
aromatic hydrocarbons, diols, flavors, aromas, ketones,
organic acids and phenols
Chiral compounds using a nitrogen selective detector

Cyclodex-B

Optical isomers of acids, alcohols, amino acids, aromatic,
diols, flavor, aromas, ketones, organic acids and phenols
Amino acids, optical isomers

CP-Chirasil-Dex CB, CP-Cyclodextrin-β-2,3,6-M-19

FAME up to C26, cis, trans, fast resolution FAME
Best separation for cis, trans FAME up to 260°C
Volatiles
Unsaturated triglycerides
Flavors, aromas, free fatty acids C1-C26
Glycols, diols, alcohols
Blood alcohol analysis
Blood alcohol analysis
Drugs of abuse confirmation
USP solvents, common solvents
Drugs of abuse confirmation
Organochlorine pesticides
Organochlorine pesticides
Chlorinated pesticides and PCBs
Trace levels of pesticides in food
and environmental samples
Chlorinated, nitrogen, phosphorus pesticides and PCBs
Chlorinated, nitrogen, phosphorus pesticides
Chlorinated, nitrogen, phosphorus pesticides,
trace level DDT and Endrin

Select FAME
CP-Sil 88 for FAME
CP-Carbowax 400 for Volatiles in Alcohol
CP-TAP CB for Triglycerides
CP-FFAP CB
CP-Wax 57 CB for Glycols and Alcohols
DB-ALC1 and DB-ALC2
HP-Blood Alcohol
DB-5ms EVDX
HP-Fast Residual Solvent
VF-DA
HP-PAS5
DB-1701P
DB-608
VF-5 Pesticides and VF-1701 Pesticides

Chiral

Foods, Flavors and Fragrances

Life Sciences

Pesticides

534

GC AND GC/MS

HP-Chiral β

CP-Chirasil-Dex CB, CP-Cyclodextrin-β-2,3,6-M-19

Rapid-MS
CP-Sil 8 CB for Pesticides
CP-Sil 19 CB for Pesticides

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

GC Column Application and Method Guides

Application

Specific Application

Agilent Phase

Polycyclic Aromatic Hydrocarbons EU regulated PAHs
PAHs in environmental and food samples
C5-C80, PAH and polar compounds
EU and EPA regulated PAHs
Petroleum
Simulated distillation using ASTM Method D2887
C6-C110+
C5-C100 simulated distallation
C5-C120 simulated distallation
PONA and PIANO analysis
Paraffins, aromatics, naphthenes and olefins C4-C20
ASTM D 5134
PONA and PIANO analysis
High temperature simulated distallation
C1-C10 hydrocarbons
C1-C6 alcohols, aromatic C6-C10
Hydrogen, sulfide, carbonyl sulfide, methanethiol,
ethanethiol and thiophenes in LPG
Polar and non-polar volatile compounds, especially
chlorosilanes with different substituents such as alkyl
groups, or groups with ether, hydroxy and nitrile bonds
C1-C6 amines, alcohols, NH3, water, solvents,
ethanol amines
C3-C20 amines, alkanol amines
C3-C8 amines and diamines
C4-C10 amines, diamines and aromatic amines
Oxygenates in C1-C10 hydrocarbons
C1-C10 hydrocarbons
Methanol, formaldehyde and formic acid in water
C1-C12 hydrocarbons
Volatile oxygenates and halogenated hydrocarbons
Semivolatiles
Polychlorinated dibenzodioxins (PCDDs)
and dibenzofurans (PCDFs)
Dioxins and dibenzo furan
EPA Semivolatiles Methods 625, 1625, 8270
and CLP protocols
EPA Semivolatiles Methods 625, 1625, 8270
and CLP protocols
PCB, detailed analysis
PCB

DB-EUPAH
Select PAH
CP-Sil PAH CB UltiMetal
VF-17ms for PAH
DB-2887
DB-HT SimDis
CP-SimDist
CP-SimDist UltiMetal
HP-PONA
CP-Sil PONA CB
CP-Sil PONA for ASTM D 5134
DB-Petro
HP-1 Aluminum Clad
Select Al2O3 MAPD
CP-TCEP for Alcohols in Gasoline
CP-Sil 5 CB for Sulfur
Select Silanes

CP-Volamine
CP-Sil 8 CB for Amines
CP-Wax for Volatile Amines and Diamines
CP-Wax 51 for Amines
CP-Lowox
GS-OxyPLOT
CP-Sil 5 CB for Formaldehyde
CP-Squalane
CP-Propox
DB-Dioxin
CP-Sil 88 for Dioxins
DB-5.625
HP-5ms Semivolatile
CP-Sil 5/C18 CB for PCB
CP-Sil 8 CB for PCB

AGILENT J&W GC AND GC/MS COLUMNS

535

GC Column Application and Method Guides

Application

Specific Application

Agilent Phase

Volatiles

EPA Methods 502.2, 524.2 and 8260
Volatile priority pollutants and residual solvents
Halogenated hydrocarbons and solvents
EPA Methods 502.2, 524.2 and 8260
EPA Method 502.2
MTBE in soil and water
Oxygenates and solvents
Total petroleum hydrocarbons (TPHs), soil analysis,
and LUFT
C5-C40 hydrocarbons
High temperature analysis and process applications
Amino acid derivatives, essential oils
Drugs, glycols, pesticides, steroids
Amines, basic compounds
Alcohols, free acids, essential oils, ethers, glycols, solvents
Generic

DB-VRX
DB-624
CP-Select 624 CB
HP-VOC
DB-502.2
DB-MTBE
CP-Select CB for MTBE
DB-TPH

Metal
Non-Bonded

Select Mineral Oil
DB-ProSteel
HP-101
HP-17
CAM
Carbowax 20M and HP-20M
SE-30 and SE-54

Agilent Phase

Composition

Application

Ultra Inert Columns
DB-1ms Ultra Inert
HP-1ms Ultra Inert
DB-5ms Ultra Inert
HP-5ms Ultra Inert
DB-35ms Ultra Inert

100% Dimethylpolysiloxane
100% Dimethylpolysiloxane
5% Phenyl 95% dimethyl arylene siloxane
5% Phenyl 95% dimethylpolysiloxane
5% Phenyl 65% dimethylpolysiloxane

Semivolatiles, halogenated compounds, pesticides, herbicides,
drugs of abuse, amines, unknown sample screening

General Application Columns

DB-1ms
HP-1ms
VF-1ms
DB-5ms
HP-5ms
VF-5ms
DB-XLB
VF-Xms
DB-35ms
VF-35ms

100% Dimethylpolysiloxane

Amines, hydrocarbons, pesticides, PCBs, phenols, sulfur
compounds, flavors and fragrances

5% Phenyl 95% dimethyl arylene siloxane
5% Phenyl 95% dimethylpolysiloxane

Semivolatiles, alkaloids, drugs, FAMEs, halogenated
compounds, pesticides, herbicides

High arylene modified
5% Phenyl 65% dimethylpolysiloxane

Pesticides, herbicides, PCBs and PAHs
Pesticides, herbicides, PCBs and PAHs
Aromatic compounds, pesticides and herbicides, sterols and
other substituted aromatic compounds

(Continued)

536

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

GC Column Application and Method Guides

Agilent Phase

Composition

Application

General Application Columns

DB-17ms
VF-17ms
VF-23ms
VF-200ms

50% Phenyl 50% dimethylpolysiloxane

Antidepressants, herbicides and pesticides

High cyanopropyl modified
Trifluoropropyl methyl

DB-225ms
VF-624ms

50% Cyanopropylphenyl 50% dimethylpolysiloxane
6% Cyanopropyl-phenyl 94% dimethylpolysiloxane

VF-1301ms

6% Cyanopropyl-phenyl 94% dimethylpolysiloxane

VF-WAXms
VF-1701ms

Polyethylene glycol
14% Cyanopropyl-phenyl 86% dimethylpolysiloxane

DB-1
HP-1
CP-Sil 5 CB
Ultra 1
Ultra 2
DB-5
HP-5
CP-Sil 8 CB
CP-Sil 13 CB

100% Dimethylpolysiloxane

FAME, solvents, sugars
Ketones, aldehydes, nitro- or chloro-containing compounds,
PAHs, unsaturated compounds, silanes and CFCs
FAMEs, alditol acetates, neutral sterols
Purgeable organic volatiles and semi-volatiles, aromatics,
halocarbons, solvents
Thin-film version of the VF-624ms suitable for volatile solvents,
pesticides, PCBs and other organic compounds requiring thin
films
Trace analysis of polar substances
Organic compounds in drinking water, base/neutrals and acids,
PCBs and chlorinated pesticides, organophosphorus pesticides
and herbicides
Amines, hydrocarbons, pesticides, PCBs, phenols, sulfur
compounds, flavors and fragrances

DB-35
HP-35
DB-17
HP-50+
CP-Sil 24 CB
DB-23
HP-88
CP-Sil 88

100% Dimethylpolysiloxane
100% Dimethylpolysiloxane
5% Phenyl 95% dimethylpolysiloxane

14% Phenyl 86% dimethylpolysiloxane

Semivolatiles, alkaloids, drugs, FAMEs, halogenated
compounds, pesticides, herbicides

35% Phenyl 65% dimethylpolysiloxane

Analysis of medium-polarity compounds where
halocarbon-sensitive detectors are used (e.g. ECD)
Amines, aromatic hydrocarbons, EPA methods, fungicides,
halogenated compounds, herbicides, pesticides, PCBs, phenols,
phthalate esters, steroids, sugars and tranquilizers
CLP-pesticides, aroclors, pharmaceuticals, drugs of abuse

50% Phenyl 50% dimethylpolysiloxane

Drugs, glycols, pesticides, steroids

50% Cyanopropyl 50% methylpolysiloxane
88% Vyanopropyl 12% aryl-polysiloxane
Highly substituted, stabilized cyanopropyl

Antidepressants, herbicides and pesticides
FAMEs
FAMEs
Dioxins, FAME, PCBs, PCDFs, pyridines and sugars
(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

537

GC Column Application and Method Guides

Agilent Phase

Composition

Application

General Application Columns

DB-200
DB-210
DB-225
CP-Sil 43 CB
DB-1301
CP-1301
DB-1701
CP-Sil 19 CB

35% Trifluoropropyl 65% dimethylpolysiloxane
50% Trifluoropropyl 50% dimethylpolysiloxane
50% Cyanopropylphenyl 50% dimethylpolysiloxane
25% Cyanopropyl 25% phenyl 50% dimethylpolysiloxane
6% Cyanopropylphenyl 94% dimethylpolysiloxane
14% Cyanopropylphenyl 86% dimethylpolysiloxane

Residual solvents, pesticides, herbicides
EPA Methods 8140 and 609
FAMEs, alditol acetates, neutral sterols
FAME, halogenated compounds, phenols and pyridines
Aroclors, alcohols, pesticides, VOCs
Herbicides, pesticides and many pharmaceutical products
Pesticides, herbicides, TMS sugars, aroclors
Trace levels of pesticide residues in food
and environmental samples

Polyethylene Glycol (PEG) Columns

DB-WAX
HP-INNOWax

Polyethylene glycol

CP-Wax 52 CB

DB-FFAP
HP-FFAP
CP-Wax 58 FFAP CB

Polyethylene glycol-acid modified

CP-Wax 57 CB

Polyethylene glycol

Solvents, glycols, alcohols
Alcohols, free organic acids, solvents, essential oils,
flavors and fragrances
Alcohols, aldehydes, anesthetics, antidepressants, aromatic
hydrocarbons, EPA methods, esters, FAME, flavors and aromas,
glycols, halogenated components, ketones, nitro compounds,
PAHs, phenols, solvents and sulfur compounds
Organic acids, alcohols, aldehydes, ketones, acrylates
FAME, flavors and aromas, free fatty acids, organic acids
and phenols
Alcohols, aromatic hydrocarbons, esters, FAME, flavors
and aromas, free fatty acids, glycols, halogenated compounds,
ketones, organic acids and solvents

PLOT Columns

CP-PoraBOND Q

Styrene-divinylbenzene copolymer

CP-PoraBOND U

Styrene-glycol methacrylate copolymer

CP-PoraPLOT Q

Styrene-divinylbenzene copolymer

CP-PoraPLOT Q-HT

Styrene-divinylbenzene copolymer

Alcohols, free fatty acids, gases, glycols, halogenated
compounds, hydrocarbons, C1-C9, ketones, solvents,
sulfur compounds
Alcohols, free fatty acids, gases, glycols, halogenated
compounds, hydrocarbons, C1-C9, ketones, solvents,
sulfur compounds
Alcohols, free fatty acids, gases, glycols, halogenated
compounds, hydrocarbons, C1-C9, ketones, solvents,
sulfur compounds
Halogenated compounds, hydrocarbons C1-C9, ketones,
oxygenated hydrocarbons, permanent gases, solvents
(Continued)

538

GC AND GC/MS

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GC Column Application and Method Guides

Agilent Phase

Composition

Application

PLOT Columns

HP-PLOT Q
GS-Q
CP-PoraPLOT U
CP-PoraPLOT S
HP-PLOT U
HP-PLOT Al2O3 KCl
GS-Alumina KCl
CP-Al2O3/KCl
CP-Al2O3/Na2SO4
HP-PLOT Al2O3 S
GS-Alumina
HP-PLOT Al2O3 M
GS-GasPro

CP-SilicaPLOT
CP-CarboBOND
CP-CarboPLOT P7
GS-CarbonPLOT
HP-PLOT Molesieve
CP-Molsieve 5Å
CP-PoraPLOT Amines

Polystyrenedivinylbenzene

Hydrocarbons including isomers, CO2, methane, air/CO, water,
polar solvents, sulfur compounds
Porous divinylbenzene homopolymer
Hydrocarbons, halogenated hydrocarbons, sulfides except
for SO2
Ethylene glycol dimethacrylate-divinylbenzene copolymer Halogenated compounds, hydrocarbons C1-C6, ketones,
oxygenated hydrocarbons, permanent gases and solvents
Vinylpyridine-divinylbenzene copolymer
Hydrocarbons, ketones
Divinylbenzene/ethylene glycol dimethacrylate
C1-C7 hydrocarbons, CO2, methane, air/CO, water, oxygenates,
amines, solvents, alcohols, ketones, aldehydes
Aluminum oxide KCl deactivated
C1-C6 hydrocarbons in natural gas, refinery gas, fuel gas,
synthetic gas, dienes
Aluminum oxide KCl deactivated
C1-C6 hydrocarbons in natural gas, refinery gas, fuel gas,
synthetic gas, dienes
Aluminium oxide
Hydrocarbons C1-C10 and impurities in hydrocarbon
mainstreams, benzene and toluene
Aluminium oxide
Hydrocarbons C1-C10 and impurities in hydrocarbon
mainstreams, benzene and toluene
Aluminum oxide "Sodium Sulfate" deactivated
C1-C6 hydrocarbons in natural gas, refinery gas, fuel gas,
synthetic gas, dienes
Aluminum oxide with proprietary deactivation
C1-C6 hydrocarbons in natural gas, refinery gas, fuel gas,
synthetic gas, dienes
Aluminium oxide
C1-C6 hydrocarbons in natural gas, refinery gas, fuel gas,
synthetic gas, dienes
Proprietary, bonded silica-based
C1-C12 hydrocarbons, CO2, trace-level sulfurs, hydride gases,
inorganic gases, halocarbons, SF6, oxygen/nitrogen separation
at -80°C
Proprietary, bonded silica-based
COS in ethylene, freons/CFCs, hydrocarbons, propylene
and sulfur gases
Active carbon
Hydrocarbons in ethylene and traces CO and CO2 in ethylene
and propylene
Active carbon
He, Xe, CO, Ne, CH4, CO2, O2/Ar, C2H6, N2, C2H4, Kr, and C2H2
Bonded monolithic carbon layer
C1-C5 hydrocarbons, CO2, air/CO, trace acetylene
in ethylene, methane
5Å molecular sieve zeolite
Permanent and noble gases. Argon and oxygen separation
at 35°C
Molecular sieve
He, H2, O2, CO, Ne, HD, N2, NO, Ar, D2, CH4, KrHT, Xe, DT,
CD4, Rn, T2
Styrene-divinylbenzene copolymer base deactivated
Amines C1-C6

AGILENT J&W GC AND GC/MS COLUMNS

539

GC Column Application and Method Guides

EPA Method
Drinking Water
EPA Method Application

501, 501.3

502.2

503.1
504.1

505

506

507

508
508.1

Recommended Column

Measurement of trihalomethanes in drinking water
GC/MS and selected ion monitoring

VF-624ms, 30 m x 0.53 mm, 3.00 µm
VF-624ms, 30 m x 0.25 mm, 1.40 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
Volatile organic compounds in water by purge and trap VF-624ms, 75 m x 0.53 mm, 3.00 µm
capillary column GC with photoionization and
VF-624ms, 30 m x 0.53 mm, 3.00 µm
electrolytic conductivity detectors in series
VF-624ms, 30 m x 0.25 mm, 1.40 µm
DB-VRX, 75 m x 0.45 mm, 2.55 µm
DB-624, 75 m x 0.45 mm, 2.55 µm
Volatile aromatic and unsaturated organic compounds DB-VRX, 30 m x 0.45 mm, 2.55 µm
in water by purge and trap gas chromatography
DB-624, 30 m x 0.45 mm, 2.55 µm
1,2-Dibromoethane (EDB) and 1,2-dibromo-3VF-1ms, 30 m x 0.32 mm, 1.00 µm
chloropropane (DB CP), GC, microextraction
VF-1701ms, 30 m x 0.32 mm, 1.00 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
Analysis of organohalide pesticides and commercial
VF-1ms, 30 m x 0.32 mm, 1.00 µm
polychlorinated biphenyl (PCB) products in water by
VF-17ms, 30 m x 0.32 mm, 0.50 µm
microextraction and GC
Determination of phthalate and adipate esters in
VF-5ms, 30 m x 0.32 mm, 0.25 µm
drinking water by liquid-liquid extraction or liquid-solid VF-1ms, 30 m x 0.32 mm, 0.25 µm
extraction and GC with photoionization detection
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
DB-608, 30 m x 0.53 mm, 0.50 µm
Determination of nitrogen and phosphorus-containing VF-5 Pesticides, 30 m x 0.25 mm, 0.25 µm
pesticides in water by GC with a nitrogen-phosphorus VF-1701 Pesticides, 30 m x 0.25 mm, 0.25 µm
detector
DB-35ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
Determination of chlorinated pesticides in water GC
VF-5 Pesticides, 30 m x 0.25 mm, 0.25 µm
with an electron capture detector
VF-1701 Pesticides, 30 m x 0.25 mm, 0.25 µm
Determination of chlorinated pesticides, herbicides, and VF-5 Pesticides, 30 m x 0.25 mm, 0.25 µm
organohalides by liquid-solid extraction and electron
DB-35ms, 30 m x 0.32 mm, 0.25 µm
capture GC
DB-XLB, 30 m x 0.32 mm, 0.50 µm

Part No.

CP9106
CP9102
124-1534
124-1334
CP9108
CP9106
CP9102
124-1574
124-1374
124-1534
124-1334
CP8926
CP9163
124-1534
124-1334
CP8926
CP8991
CP8955
CP8924
122-5532
125-5532
125-6837
CP9074
CP9070
122-3832
122-5532
CP9074
CP9070
CP9074
123-3832
123-1236
(Continued)

540

GC AND GC/MS

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GC Column Application and Method Guides

Drinking Water
EPA Method Application

Recommended Column

Part No.

515

DB-35ms, 30 m x 0.32 mm, 0.25 µm

123-3832

VF-1701ms, 30 m x 0.25 mm, 0.25 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm

CP9151
CP8944

VF-1701ms, 30 m x 0.25 mm, 0.25 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm

CP9151
CP8944

VF-5ms, 30 m x 0.25 mm, 1.00 µm

CP8946

VF-624ms, 30 m x 0.53 mm, 3.00 µm
VF-624ms, 75 m x 0.53 mm, 3.00 µm
VF-5ms, 30 m x 0.32 mm, 1.00 µm
DB-VRX, 60 m x 0.25 mm, 1.40 µm
DB-624, 60 m x 0.25 mm, 1.4 µm
HP-VOC, 60 m x 0.20 mm, 1.10 µm
DB-VRX, 20 m x 0.18 mm, 1.00 µm
DB-624, 20 m x 0.18 mm, 1.00 µm
VF-5 Pesticides, 30 m x 0.25 mm, 0.25 µm
HP-5ms, 30 m x 0.25 mm, 0.50 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm

CP9106
CP9108
CP8957
122-1564
122-1364
19091R-306
121-1524
121-1324
CP9074
19091S-133
CP8944

VF-5ms, 30 m x 0.25 mm, 0.25 µm

CP8944

VF-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-XLB, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
VF-5ms, 15 m x 0.25 mm, 0.25 µm

CP8944
122-5532
122-1232
125-5532
CP8939

515.3

515.4

521

524.2

Determination of chlorinated herbicides in drinking
water
Determination of chlorinated acids in drinking water by
liquid-liquid extraction, derivatization and GC with
electron capture detection
Determination of chlorinated acids in drinking water by
liquid-liquid microextraction, derivatization, and fast GC
with electron capture detection
Determination of nitrosamines in drinking water by solid
phase extraction and capillary column gas
chromatography with large volume injection and
chemical ionization tandem mass spectrometry
(MS/MS)
Measurement of purgeable organic compounds in
water by capillary GC/MS

525, 525.2

Determination of organic compounds in drinking water
by liquid-solid extraction and capillary column GC/MS

526

Determination of selected semivolatile organic
compounds in drinking water by solid phase extraction
and capillary column GC/MS
Determination of selected pesticides and flame
retardants in drinking water by solid phase extraction
and capillary column GC/MS
Determination of phenols in drinking water by solid
phase extraction and capillary column GC/MS

527

528

529

Determination of explosives and related compounds in
drinking water by solid phase extraction and capillary
column GC/MS

(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

541

GC Column Application and Method Guides

Drinking Water
EPA Method Application

Recommended Column

Part No.

551

VF-1301ms, 30 m x 0.25 mm, 1.00 µm
DB-5ms, 30 m x 0.25 mm, 1.00 µm
DB-1, 30 m x 0.25 mm, 1.00 µm

CP9054
122-5533
122-1033

VF-1ms, 30 m x 0.25 mm, 1.00 µm
VF-1301ms, 30 m x 0.25 mm, 1.00 µm
DB-5ms, 30 m x 0.25 mm, 1.00 µm
DB-1, 30 m x 0.25 mm, 1.00 µm
Determination of Haloacetic Acids in Drinking Water by VF-5ms, 30 m x 0.25 mm, 0.25 µm
Liquid-Liquid Extraction, Derivatization, and Gas
DB-35ms, 30 m x 0.32 mm, 0.25 µm
Chromatography with Electron Capture Detection
DB-XLB, 30 m x 0.32 mm, 0.50 µm
Determination of Haloacetic Acids and Dalapon in
DB-35ms, 30 m x 0.32 mm, 0.25 µm
Drinking Water by Ion Exchange Liquid-Solid Extraction DB-XLB, 30 m x 0.32 mm, 0.50 µm
and Gas Chromatography with an Electron Capture
Detector
Determination of haloacetic acids and dalapon in
VF-1701ms, 30 m x 0.25 mm, 0.25 µm
drinking water by liquid-liquid extraction, derivatization VF-5ms, 30 m x 0.25 mm, 0.25 µm
GC with electron capture detection
DB-35ms, 30 m x 0.32 mm, 0.25 µm
DB-XLB, 30 m x 0.32 mm, 0.50 µm
Determination of haloacetic acids and dalapon in
VF-1701ms, 30 m x 0.25 mm, 0.25 µm
drinking water by liquid-liquid microextraction,
VF-5ms, 30 m x 0.25 mm, 0.25 µm
derivatization, and GC with electron capture detection
Determination of carbonyl compounds in drinking water VF-1701ms, 30 m x 0.25 mm, 0.25 µm
by pentafluorobenzylhydroxylamine derivatization and
VF-5ms, 30 m x 0.25 mm, 0.25 µm
capillary GC with electron capture detection

CP8913
CP9054
122-5533
122-1033
CP8944
123-3832
123-1236
123-3832
123-1236

551.1

552

552.1

552.2

552.3

556

542

Determination of Chlorination Disinfection Byproducts
and Chlorinated Solvents in Drinking Water by LiquidLiquid Extraction and Gas Chromatography with
Electron Capture Detection
Determination of chlorination disinfection byproducts,
chlorinated solvents, and halogenated
pesticides/herbicides in drinking water by liquid-liquid
extraction and GC with electron-capture detection

GC AND GC/MS

www.agilent.com/chem/gccolumns l 01357 522 961
www.crawfordscientific.com

CP9151
CP8944
123-3832
123-1236
CP9151
CP8944
CP9151
CP8944

GC Column Application and Method Guides

Waste Water
EPA Method Application

Column

Part No.

601

Purgeable Halocarbons

602

Purgeable aromatics

603

Acrolein and Acrylonitrile

604

Phenols

605

Benzidines

606

Phthalate esters

607

Nitrosamines

608

Organochlorine pesticides and PCBs

VF-624ms, 75 m x 0.53 mm, 3.00 µm
VF-624ms, 60 m x 0.32 mm, 1.80 µm
VF-624ms, 30 m x 0.25 mm, 1.40 µm
DB-VRX, 75 m x 0.45 mm, 2.55 µm
DB-624, 75 m x 0.45 mm, 2.55 µm
VF-624ms, 75 m x 0.53 mm, 3.00 µm
VF-624ms, 30 m x 0.25 mm, 1.40 µm
VF-624ms, 30 m x 0.25 mm, 1.40 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
VF-WAXms, 30 m x 0.25 mm, 1.00 µm
VF-624ms, 30 m x 0.25 mm, 1.40 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
VF-624ms, 60 m x 0.32 mm, 1.80 µm
VF-624ms, 60 m x 0.25 mm, 1.40 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-XLB, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
DB-608, 30 m x 0.53 mm, 0.50 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
DB-608, 30 m x 0.53 mm, 0.50 µm
CP-Sil 8 CB for Amines, 30 m x 0.32 mm, 1.00 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
VF-5 Pesticides, 30 m x 0.25 mm, 0.25 µm
VF-1701 Pesticides, 30 m x 0.25 mm, 0.25 µm
VF-17ms, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.32 mm, 0.25 µm
DB-XLB, 30 m x 0.32 mm, 0.50 µm

CP9108
CP9105
CP9102
124-1574
124-1374
CP9108
CP9102
CP9102
124-1534
124-1334
CP9206
CP9102
124-1534
124-1334
CP9105
CP9103
122-5532
122-1232
125-5532
122-5532
125-5532
125-6837
CP8944
122-5532
125-5532
125-6837
CP7596
122-5532
125-5532
CP9074
CP9070
CP8982
123-3832
123-1236
(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

543

GC Column Application and Method Guides

Waste Water
EPA Method Application

Column

Part No.

609

Nitroaromatics and Isophorone

610

Polynuclear Aromatic Hydrocarbons

611

Haloethers

612

Chlorinated Hydrocarbons

613

2,3,7,8-Tetrachlorodibenzo-p-dioxin

614

The Determination of Organophosphorus Pesticides
in Municipal and Industrial Wastewater

615

Chlorinated herbicides

619

Triazine pesticides

622

The Determination of Organophosphorus Pesticides
in Municipal and Industrial Wastewater

VF-5ms, 30 m x 0.53 mm, 1.50 µm
VF-5ms, 30 m x 0.25 mm, 0.50 µm
HP-5ms, 30 m x 0.25 mm, 0.50 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
DB-608, 30 m x 0.53 mm, 0.50 µm
VF-17ms, 30 m x 0.25 mm, 0.25 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.32 mm, 0.25 µm
DB-1ms, 30 m x 0.25 mm, 0.25 µm
VF-5ms, 30 m x 0.53 mm, 1.50 µm
VF-5ms, 30 m x 0.25 mm, 0.50 µm
VF-5ms, 30 m x 0.25 mm, 0.10 µm
VF-35ms, 30 m x 0.25 mm, 0.25 µm
VF-200ms, 30 m x 0.25 mm, 1.00 µm
DB-5ms, 30 m x 0.32 mm, 0.50 µm
HP-5ms, 30 m x 0.32 mm, 0.50 µm
DB-1, 30 m x 0.32 mm, 0.50 µm
CP-Sil 88 for Dioxins, 50 m x 0.25 mm, 0.20 µm
VF-5ms, 60 m x 0.25 mm, 0.10 µm
DB-35ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
VF-1701 Pesticides, 30 m x 0.25 mm, 0.25 µm
VF-5 Pesticides, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.32 mm, 0.25 µm
VF-17ms, 30 m x 0.25 mm, 0.50 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm

CP8976
CP8945
19091S-133
125-5532
125-6837
CP8982
CP8944
122-5532
123-5532
122-0132
CP8976
CP8945
CP8943
CP8877
CP8860
123-5536
19091S-113
123-103E
CP7588
CP8948
122-3832
122-5532
CP9070
CP9074
123-3832
CP8983
CP8944
122-3832
122-5532
122-3832
122-5532
(Continued)

544

GC AND GC/MS

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GC Column Application and Method Guides

Waste Water
EPA Method Application

Column

Part No.

624

Purgeables

625

Base/neutrals and acids

1613

Tetra- through octa-chlorinated dioxins and furans
by isotope dilution HRGC/HRMS

1624
1625

Volatile organic compounds by isotope dilution GC/MS
Semivolatile organic compounds by isotope dilution
GC/MS
Volatile Halogenated Organic Compounds List by
EPA Method 8021

VF-624ms, 75 m x 0.53 mm, 3.00 µm
VF-624ms, 60 m x 0.32 mm, 1.80 µm
VF-624ms, 30 m x 0.25 mm, 1.40 µm
DB-VRX, 60 m x 0.25 mm, 1.40 µm
DB-624, 60 m x 0.25 mm, 1.4 µm
HP-VOC, 60 m x 0.20 mm, 1.10 µm
DB-VRX, 20 m x 0.18 mm, 1.00 µm
DB-624, 20 m x 0.18 mm, 1.00 µm
VF-5 Pesticides, 30 m x 0.25 mm, 0.25 µm
VF-1701 Pesticides, 30 m x 0.25 mm, 0.25 µm
VF-200ms, 30 m x 0.25 mm, 0.25 µm
HP-5ms, 30 m x 0.25 mm, 0.50 µm
VF-5ms, 60 m x 0.25 mm, 0.25 µm
CP-Sil 88 for Dioxins, 50 m x 0.25 mm, 0.20 µm
VF-624ms, 60 m x 0.25 mm, 1.40 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm

CP9108
CP9105
CP9102
122-1564
122-1364
19091R-306
121-1524
121-1324
CP9074
CP9070
CP8858
19091S-133
CP8960
CP7588
CP9103
CP8944

DB-VRX, 75 m x 0.45 mm, 2.55 µm
DB-624, 75 m x 0.45 mm, 2.55 µm
DB-VRX, 75 m x 0.45 mm, 2.55 µm
DB-624, 75 m x 0.45 mm, 2.55 µm

124-1574
124-1374
124-1574
124-1374

8010
8021

Volatile Halogenated & Aromatic Organic Compounds

AGILENT J&W GC AND GC/MS COLUMNS

545

GC Column Application and Method Guides

Solid Waste
EPA Method

Application

Column

Part No.

8010

Volatile Halogenated Organic Compounds List by
EPA Method 8021

8011

1,2-Dibromoethane and 1,2-dibromo-3chloropropane by microextraction and GC

8015

Nonhalogenated organics by GC

8015c

Nonhalogenated organics by GC

8020

Volatile Aromatic Organic Compounds List by EPA
Method 8021

DB-VRX, 75 m x 0.45 mm, 2.55 µm
DB-624, 75 m x 0.45 mm, 2.55 µm
VF-1ms, 30 m x 0.32 mm, 0.25 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
VF-WAXms, 30 m x 0.53 mm, 1.00 µm
CP-Sil 8 CB, 30 m x 0.53 mm, 1.50 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
DB-VRX, 75 m x 0.45 mm, 2.55 µm
DB-624, 75 m x 0.45 mm, 2.55 µm
VF-624ms, 60 m x 0.53 mm, 3.00 µm
VF-624ms, 60 m x 0.25 mm, 1.40 µm
CP-PoraBOND Q, 25 m x 0.53 mm, 10.00 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
CP-Wax 58 FFAP CB, 25 m x 0.53 mm, 2.00 µm
VF-WAXms, 15 m x 0.53 mm, 1.00 µm

124-1574
124-1374
CP8924
124-1534
124-1334
124-1534
124-1334
CP9215
CP8736
124-1534
124-1334
124-1574
124-1374
CP9107
CP9103
CP7354
124-1534
124-1334
CP7654
CP9226

DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-XLB, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
VF-5ms, 30 m x 0.53 mm, 1.50 µm
VF-1701ms, 30 m x 0.53 mm, 1.00 µm
VF-17ms, 30 m x 0.53 mm, 1.00 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
DB-608, 30 m x 0.53 mm, 0.50 µm

122-5532
122-1232
125-5532
CP8976
CP9171
CP9001
122-5532
125-5532
125-6837

8021, CLP Volamines Volatile Halogenated & Aromatic Organic
Compounds
8021b

Aromatic and halogenated volatiles by GC

8031

Acrylonitrile by GC

8032
8033
8040, 8041

Acrylamide by GC
Acetonitrile by GC with
nitrogen-phosphorus detection
Phenols by Gas Chromatography

8041a

Phenols by GC

8060

Phthalate esters

(Continued)

546

GC AND GC/MS

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GC Column Application and Method Guides

Solid Waste
EPA Method

Application

Column

Part No.

8061

Phthalate esters by GC with electron capture
detection (GC/ECD)

8070

Nitrosamines by Gas Chromatography

8070a

Nitrosamines by GC

8081

Organochlorine pesticides by GC

8081a

Organochlorine Pesticides by Gas Chromatography

VF-5ms, 30 m x 0.53 mm, 1.50 µm
VF-1701ms, 30 m x 0.53 mm, 1.00 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
DB-608, 30 m x 0.53 mm, 0.50 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
CP-Sil 8 CB for Amines, 30 m x 0.53 mm, 1.00 µm
VF-17ms, 30 m x 0.53 mm, 1.50 µm
VF-5ms, 30 m x 0.25 mm, 1.00 µm
VF-35ms, 30 m x 0.25 mm, 1.00 µm
VF-35ms, 30 m x 0.53 mm, 0.50 µm
VF-1701ms, 30 m x 0.53 mm, 1.00 µm
VF-5ms, 30 m x 0.53 mm, 1.50 µm
DB-35ms, 30 m x 0.32 mm, 0.25 µm
DB-XLB, 30 m x 0.32 mm, 0.50 µm
DB-35ms, 30 m x 0.32 mm, 0.25 µm
DB-XLB, 30 m x 0.32 mm, 0.50 µm
VF-5ms, 30 m x 0.25 mm, 1.00 µm
VF-35ms, 30 m x 0.25 mm, 1.00 µm
VF-35ms, 30 m x 0.53 mm, 0.50 µm
VF-1701ms, 30 m x 0.53 mm, 1.00 µm
VF-5ms, 30 m x 0.53 mm, 1.50 µm
HP-5ms, 30 m x 0.25 mm, 0.50 µm
DB-5ms, 30 m x 0.53 mm, 1.50 µm
DB-608, 30 m x 0.53 mm, 0.50 µm
VF-5ms, 30 m x 0.53 mm, 1.50 µm
VF-1701ms, 30 m x 0.53 mm, 1.00 µm
VF-5ms, 15 m x 0.53 mm, 1.50 µm
VF-1ms, 15 m x 0.53 mm, 1.50 µm
VF-200ms, 15 m x 0.53 mm, 1.00 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.32 mm, 0.25 µm
DB-1ms, 30 m x 0.25 mm, 0.25 µm

CP8976
CP9171
122-5532
125-5532
125-6837
122-5532
125-5532
CP7597
CP9002
CP8946
CP8879
CP8887
CP9171
CP8976
123-3832
123-1236
123-3832
123-1236
CP8946
CP8879
CP8887
CP9171
CP8976
19091S-133
125-5532
125-6837
CP8976
CP9171
CP8973
CP8967
CP8866
CP8944
122-5532
123-5532
122-0132

8082, CLP Pesticides Polychlorinated Biphenyls (PCBs)
by Gas Chromatography
8082a

Polychlorinated biphenyls (PCBs) by GC

8090

Nitroaromatics and Isophorone

8091

Nitroaromatics and cyclic ketones by GC

8095

Explosives by GC

8100

Polynuclear aromatic hydrocarbons

(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

547

GC Column Application and Method Guides

Solid Waste
EPA Method

Application

Column

Part No.

8111

Haloethers by GC

8120

Chlorinated hydrocarbons by Gas Chromatography

8121

Chlorinated hydrocarbons by GC:
capillary column technique

8131

Aniline and selected derivatives by GC

8140

Organophosphorus Pesticides by GC-NPD

8141a

Organophosphorus compounds by gas
chromatography: capillary column technique

8141b

Organophosphorus compounds by GC

8150
8151

Chlorinated herbicides
Chlorinated herbicides by GC using methylation
or pentafluorobenzylation derivatization:
capillary column technique
Chlorinated herbicides by GC using methylation
or pentafluorobenzylation derivatization

VF-5ms, 15 m x 0.53 mm, 1.50 µm
VF-1701ms, 30 m x 0.53 mm, 1.00 µm
DB-5ms, 30 m x 0.32 mm, 0.50 µm
HP-5ms, 30 m x 0.32 mm, 0.50 µm
DB-1, 30 m x 0.32 mm, 0.50 µm
VF-200ms, 30 m x 0.53 mm, 1.00 µm
VF-WAXms, 30 m x 0.53 mm, 1.00 µm
VF-5ms, 30 m x 0.53 mm, 1.50 µm
VF-1701ms, 30 m x 0.53 mm, 1.00 µm
DB-5ms, 30 m x 0.32 mm, 0.50 µm
HP-5ms, 30 m x 0.32 mm, 0.50 µm
DB-1, 30 m x 0.32 mm, 0.50 µm
VF-5ms, 30 m x 0.25 mm, 0.25 µm
CP-Sil 8 CB for Amines, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.25 mm, 0.25 µm
DB-5ms, 30 m x 0.25 mm, 0.25 µm
VF-200ms, 30 m x 0.53 mm, 1.00 µm
VF-35ms, 30 m x 0.53 mm, 1.00 µm
VF-5ms, 30 m x 0.53 mm, 1.00 µm
VF-1ms, 30 m x 0.53 mm, 1.00 µm
DB-35ms, 30 m x 0.32 mm, 0.25 µm
DB-35ms, 30 m x 0.32 mm, 0.25 µm

CP8973
CP9171
123-5536
19091S-113
123-103E
CP8868
CP9215
CP8976
CP9171
123-5536
19091S-113
123-103E
CP8944
CP7598
122-3832
122-5532
122-3832
122-5532
CP8868
CP8888
CP8975
CP8969
123-3832
123-3832

VF-5 Pesticides, 30 m x 0.25 mm, 0.25 µm
VF-5ms, 30 m x 0.32 mm, 1.00 µm
VF-35ms, 30 m x 0.25 mm, 0.25 µm
VF-1701 Pesticides, 30 m x 0.25 mm, 0.25 µm
VF-35ms, 30 m x 0.53 mm, 1.00 µm
VF-1701ms, 30 m x 0.53 mm, 1.00 µm
DB-VRX, 20 m x 0.18 mm, 1.00 µm
DB-624, 20 m x 0.18 mm, 1.00 µm
DB-VRX, 60 m x 0.25 mm, 1.40 µm
DB-624, 60 m x 0.25 mm, 1.4 µm
HP-VOC, 60 m x 0.20 mm, 1.10 µm

CP9074
CP8957
CP8877
CP9070
CP8888
CP9171
121-1524
121-1324
122-1564
122-1364
19091R-306

8151b

8240

Volatile Chlorinated and Aromatic Hydrocarbons

(Continued)

548

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GC Column Application and Method Guides

Solid Waste
EPA Method

Application

Column

Part No.

8260/CLP-VOCs

Volatile Organic Compounds by Gas
Chromatography/Mass Spectroscopy (GC/MS):
Capillary Column Technique Method

8260b

Volatile organic compounds by GC/MS

8261

Volatile organic compounds by vacuum distillation
in combination with GC/MS spectrometry
(VD/GC/MS)
Semivolatile Organic Compounds by Gas
Chromatography/Mass Spectrometry (GC/MS)
Semivolatile organic compounds by GC/MS

DB-VRX, 60 m x 0.25 mm, 1.40 µm
DB-624, 60 m x 0.25 mm, 1.4 µm
HP-VOC, 60 m x 0.20 mm, 1.10 µm
DB-VRX, 20 m x 0.18 mm, 1.00 µm
DB-624, 20 m x 0.18 mm, 1.00 µm
VF-624ms, 75 m x 0.53 mm, 3.00 µm
VF-5ms, 30 m x 0.25 mm, 1.00 µm
VF-624ms, 60 m x 0.32 mm, 1.80 µm
VF-624ms, 60 m x 0.53 mm, 3.00 µm
VF-624ms, 60 m x 0.25 mm, 1.40 µm

122-1564
122-1364
19091R-306
121-1524
121-1324
CP9108
CP8946
CP9105
CP9107
CP9103

HP-5ms, 30 m x 0.25 mm, 0.50 µm

19091S-133

8270
8270d

8275a

8280b

8290b

8410

8430

VF-5ms, 30 m x 0.25 mm, 0.25 µm
VF-5ms, 30 m x 0.25 mm, 0.50 µm
VF-5ms, 30 m x 0.25 mm, 1.00 µm
Semivolatile organic compounds (PAHs and PCBs) VF-5ms, 30 m x 0.25 mm, 0.25 µm
in soils/sludges and solid wastes using thermal
VF-5ms, 30 m x 0.25 mm, 0.50 µm
extraction/gas chromatography/mass spectrometry
VF-5ms, 30 m x 0.25 mm, 1.00 µm
(TE/GC/MS)
Polychlorinated dibenzo-p-dioxins (PCDDs) and
CP-Sil 8 CB, 30 m x 0.25 mm, 0.25 µm
polychlorinated dibenzofurans (PCDfs) by high
resolution gas chromatography/low resolution
mass spectrometry (HRGC/LRMS)
Polychlorinated dibenzo-p-dioxins (PCDDs) and
CP-Sil 8 CB, 30 m x 0.25 mm, 0.25 µm
polychlorinated dibenzofurans (PCDfs) by high
CP-Sil 88 for Dioxins, 50 m x 0.25 mm, 0.20 µm
resolution gas chromatography/high resolution
mass spectrometry (HRGC/HRMS)
Gas chromatography/Fourier transform infrared
VF-5ms, 30 m x 0.32 mm, 0.25 µm
(GC/FT-IR) spectrometry for semivolatile organics:
capillary column
Analysis of bis(2-chloroethyl) ether and hydrolysis VF-WAXms, 30 m x 0.53 mm, 1.00 µm
products by direct aqueous injection (GC/FT-IR)

CP8944
CP8945
CP8946
CP8944
CP8945
CP8946
CP8751

CP8751
CP7588
CP8955

CP9215

AGILENT J&W GC AND GC/MS COLUMNS

549

GC Column Application and Method Guides

United States Pharmacopoeia (USP) GC Phases
USP Phase Composition
G1 Dimethylpolysiloxane oil
G2

Dimethylpolysiloxane gum

G3

50% Phenyl 50% methylpolysiloxane

G5
G6
G7
G8
G14
G15
G16
G17

3-cyanopropyl polysiloxane
Trifluoropropylmethylpolysilicone
50% 3-cyanopropyl 50% phenylmethylsilicone
80% Bis(3-cyanopropyl) 20% 3-cyanopropylphenylpolysiloxane
or 90% 3-cyanopropyl 10% phenylmethylsiloxane
Polyethylene glycol (average molecular weight of 950-1,050)
Polyethylene glycol (average molecular weight of 3,000-3,700)
Polyethylene glycol (average molecular weight of 15,000)
75% Phenyl 25% methylpolysiloxane

G19
G20
G25
G27

25% Phenyl 25% cyanopropylmethylsilicone
Polyethylene glycol (average molecular weight of 380-420)
Polyethylene glycol TPA (Carbowax 20M terephthalic acid)
5% Phenyl 95% methylpolysiloxane

G28
G32
G35
G36

25% Phenyl 75% methylpolysiloxane
20% Phenylmethyl 80% dimethylpolysiloxane
Polyethylene glycol & diepoxide esterified with nitroterepthalic acid
1% Vinyl 5% phenylmethylpolysiloxane

G38 Phase G1 plus a tailing inhibitor
G39 Polyethylene glycol (average molecular weight of 1,500)
G41 Phenylmethyldimethylsilicone (10% phenyl substituted)
G42 35% Phenyl 65% dimethylvinylsiloxane
G43 6% Cyanopropylphenyl 94% dimethylpolysiloxane
G45 Divinylbenzene-ethylene glycol-dimethacrylate
G46 14% Cyanopropylphenyl 86% methylpolysiloxane

Agilent Phase Recommendation
HP-1*, DB-1*, HP-1ms*, DB-1ms*, VF-1ms, CP-Sil 5 CB,
CP-Sil 5 CB Low Bleed/MS
HP-1*, DB-1*, HP-1ms*, DB-1ms*, VF-1ms, CP-Sil 5 CB,
CP-Sil 5 CB Low Bleed/MS, CP-SimDist
DB-17*, HP-50+*, VF-17ms, CP-Sil 24 CB,
CP-Sil 24 CB Low Bleed/MS
DB-23, VF-23ms, Select for FAME, CP-Sil 88
DB-200, DB-210, VF-200ms
DB-225, DB-225ms, CP-Sil 43 CB
HP-88, VF-23ms
DB-WAX, VF-WAXms, CP-Wax 52 CB
DB-WAX, VF-WAXms, CP-Wax 52 CB
DB-WAX*, VF-WAXms, CP-Wax 52 CB
DB-17, HP-50+, VF-17ms, CP-Sil 24 CB,
CP-Sil 24 CB Low Bleed/MS
DB-225*, DB-225ms, CP-Sil 43 CB
DB-WAX, VF-WAXms, CP-Wax 52 CB
DB-FFAP*, HP-FFAP*, CP-Wax 58 (FFAP) CB, CP-FFAP CB
DB-5*, HP-5*, HP-5ms*, DB-5ms, VF-5ms, VF-5ht, CP-Sil 8 CB,
CP-Sil 8 CB Low Bleed/MS
DB-35, HP-35, DB-35ms, VF-35ms
DB-35, HP-35, DB-35ms, VF-35ms
DB-FFAP*, HP-FFAP*, CP-Wax 58 (FFAP) CB, CP-FFAP CB
DB-5, HP-5, HP-5ms, DB-5ms, VF-5ms, VF-5ht, CP-Sil 8 CB,
CP-Sil 8 CB Low Bleed/MS
DB-1, HP-1, HP-1ms, DB-1ms, VF-1ms, CP-Sil 5 CB,
CP-Sil 5 CB Low Bleed/MS
DB-WAX, VF-WAXms, CP-Wax 52 CB
DB-5, HP-5, HP-5ms, DB-5ms, VF-5ms, VF-5ht, CP-Sil 8 CB,
CP-Sil 8 CB Low Bleed/MS
DB-35*, HP-35*, DB-35ms, VF-35ms
DB-624*, DB-1301, VF-624ms, VF-1301ms, CP-1301,
CP-Select 624 CB
HP-PLOT U*, CP-PoraBOND U, CP-PoraPLOT U
DB-1701*, VF-1701ms, CP-Sil 19 CB, CP-Sil 19 CB Low Bleed/MS

*Indicates an exact equivalent

Tips & Tools
Gain extra confidence to meet high standards with Agilent's solution
for the revised USP <467>. Visit www.agilent.com/chem/usp467an

550

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GC Column Application and Method Guides

ASTM Methods
Method

Title

Recommended Agilent Column

Part No.

D 1945

Standard Test Method for the Analysis
of Natural Gas by GC

D 1946

Standard Test Method for the Analysis
of Reformed Gas by GC

D 1983

Standard Test Method for Fatty Acid Composition
by Gas-Liquid Chromatography of Methyl Esters
Standard Test Method for the Analysis of Liquified
Petroleum (LP) Gases and Propene Concentrates
by GC
Standard Test Methods for Pentaerythritol
Standard Test Method for Analysis of High-Purity
n-Heptane and Isooctane by Capillary GC
Standard Test Method for C8
Aromatic Hydrocarbons by GC
Standard Test Method for Trace Impurities
in Monocyclic Aromatic Hydrocarbons by GC
Standard Test Method for Butadiene Dimer
and Styrene in Butadiene Concentrates by GC

HP PLOT, 15 m x 0.53 mm, 50 µm
HP PLOT Q, 15 m x 0.53 mm, 40 µm
CP-Molsieve 5Å, 10 m x 0.53 mm, 50.00 µm
CP-PoraPLOT Q-HT, 10 m x 0.53 mm, 20.00 µm
HP PLOT, 15 m x 0.53 mm, 50 µm
HP PLOT Q, 15 m x 0.53 mm, 40 µm
CP-Molsieve 5Å, 10 m x 0.53 mm, 50.00 µm
CP-Molsieve 5Å, 25 m x 0.25 mm, 30.00 µm
DB-WAX, 30 m x 0.25 mm, 0.25 µm

19095P-MS9
19095P-QO3
CP7537
CP7558
19095P-MS9
19095P-QO3
CP7537
CP7533
122-7032

HP PLOT Al2O3 "KCl", 30 m x 0.53 mm, 15 µm
HP PLOT Al2O3 "S", 30 m x 0.53 mm, 15 µm

19095P-K23
19095P-S23

CP-Sil 5 CB, 30 m x 0.53 mm, 1.50 µm
DB-1, 60 m x 0.25 mm, 0.50 µm

CP8735
122-106E

HP-INNOWax, 60 m x 0.25 mm, 0.25 µm

19091N-136

HP-INNOWax, 60 m x 0.32 mm, 0.25 µm

19091N-116

DB-1, 30 m x 0.53 mm, 5.00 µm
CP-Sil 5 CB, 30 m x 0.53 mm, 1.50 µm
DB-1, 30 m x 0.53 mm, 5.00 µm
GS-Alumina, 30 m x 0.53 mm,
CP-Al2O3/KCl, 50 m x 0.53 mm, 10.00 µm
CP-Sil 88 for FAME, 50 m x 0.25 mm, 0.20 µm

125-1035
CP8735
125-1035
115-3532
CP7518
CP7488

HP PLOT, 30 m x 0.53 mm, 50 µm
CP-CarboBOND, 25 m x 0.53 mm, 10.00 µm
GS-GasPro, 60 m x 0.32 mm,

19095P-MS0
CP7374
113-4362

CP-Sil 8 CB, 25 m x 0.32 mm, 0.40 µm
CP-FFAP CB, 25 m x 0.53 mm, 1.00 µm

CP5850
CP7486

D 2163

D 2195
D 2268
D 2306
D 2360
D 2426
D 2427

Standard Test Method for Determination of
C2 through C5 Hydrocarbons in Gasoline by GC

D 2245

Standard Test Method for Identification of Oils
and Oil Acids in Solvent-Reducible Paints
Standard Test Method for Noncondensable Gases
in C2 and Lighter Hydrocarbon Products by GC

D 2504
D 2505

D 2580

Standard Test Method for Ethylene, Other
Hydrocarbons, and Carbon Dioxide in High-Purity
Ethylene by GC
Standard Test Method for Phenols in Water
by Gas-Liquid Chromatography

(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

551

GC Column Application and Method Guides

ASTM Methods
Method

Title

Recommended Agilent Column

Part No.

D 2593

Standard Test Method for Butadiene Purity and
Hydrocarbon Impurities by GC

D 2712

Standard Test Method for for Hydrocarbon Traces
in Propylene Concentrates by GC
Standard Practices for Uniformity of Traffic Paint
Vehicle Solids by Spectroscopy and Gas
Chromatography
Standard Test Method for Purity of Methyl Ethyl
Ketone by GC

GS-Alumina, 30 m x 0.53 mm
CP-Al2O3/KCl, 25 m x 0.32 mm, 5.00 µm
CP-Al2O3/KCl, 50 m x 0.53 mm, 10.00 µm
GS-Alumina, 50 m x 0.53 mm

115-3532
CP7515
CP7518
115-3552

CP-Sil 88 for FAME, 50 m x 0.25 mm, 0.20 µm

CP7488

DB-WAX, 30 m x 0.53 mm, 1.00 µm
DB-210, 15 m x 0.53 mm, 1 µm
CP-WAX 52 CB, 30 m x 0.32 mm, 0.50 µm
CP-WAX 52 CB, 30 m x 0.53 mm, 1.00 µm
DB-2887, 10 m x 0.53 mm, 3.00 µm
CP-SimDist UltiMetal, 5 m x 0.53 mm, 0.88 µm
CP-SimDist UltiMetal, 10 m x 0.53 mm, 2.65 µm
CP-SimDist UltiMetal, 5 m x 0.53 mm, 0.17 µm
HP-1, 10 m x 0.53 mm, 0.88 µm
HP-1, 5 m x 0.53 mm, 0.88 µm
CP-Select 624 CB, 30 m x 0.32 mm, 1.80 µm
CP-Select 624 CB, 75 m x 0.53 mm, 3.00 µm
CP-WAX 52 CB, 30 m x 0.32 mm, 0.50 µm
CP-WAX 52 CB, 30 m x 0.53 mm, 1.00 µm
DB-1, 60 m x 0.32 mm, 0.50 µm

125-7032
125-0212
CP8763
CP8738
125-2814
CP7570
CP7582
CP7532
19095Z-021
19095Z-020
CP7414
CP7417
CP8763
CP8738
123-106E

CP-Sil 5 CB, 30 m x 0.32 mm, 1.00 µm
CP-Sil 5 CB, 30 m x 0.53 mm, 1.50 µm
DB-624, 30 m x 0.53 mm, 3.00 µm

CP8760
CP8735
125-1334

CP-PoraPLOT Q, 25 m x 0.53 mm, 20.00 µm
CP-WAX 52 CB, 30 m x 0.53 mm, 1.00 µm

CP7554
CP8738

CP-Sil 5 CB, 30 m x 0.32 mm, 3.00 µm
CP-Sil 5 CB, 30 m x 0.53 mm, 3.00 µm
DB-WAX, 30 m x 0.53 mm, 1.00 µm
DB-624, 30 m x 0.45 mm, 2.55 µm
CP-WAX 52 CB, 60 m x 0.53 mm, 1.00 µm
HP-1ms, 30 m x 0.32 mm, 1.00 µm

CP8687
CP8677
125-7032
124-1334
CP8798
19091S-713

D 2743

D 2804

D 2887

Standard Test Method for Boiling Range Distribution
of Petroleum Fractions by GC

Extended D 2887 Standard Test Method for Boiling Range Distribution
of Petroleum Fractions by GC, to C60
D 2908

Standard Practice for Measuring Volatile Organic
Matter in Water by Aqueous-Injection GC

D 3054

Standard Test Method for Analysis
of Cyclohexane by GC
Standard Practice for Qualitative Identification
of Polymers in Emulsion Paints

D 3168
D 3257
D 3271

D 3328

Standard Test Method for Aromatics in Mineral
Spirits by GC
Standard Practice for Direct Injection of
Solvent-Reducible Paints into a Gas Chromatograph
for Solvent Analysis
Standard Test Methods for Comparison of
Waterborne Petroleum Oils by Gas Chromatography

D 3329

Standard Test Method for Purity of Methyl Isobutyl
Ketone by GC

D 3432

Standard Test Method for Unreacted Toluene
Diisocyanates in Urethane Prepolymers
and Coating Solutions by GC

(Continued)

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GC Column Application and Method Guides

ASTM Methods
Method

Title

Recommended Agilent Column

Part No.

D 3447

Standard Test Method for Purity of Halogentated
Organic Solvents
Standard Practice for Rubber – Identification
by Pyrolysis-Gas Chromatography
Standard Test Method for Purity of Monomeric
Plasticizers by Gas Chromatography

DB-624, 30 m x 0.53 mm, 3.00 µm

125-1334

CP-Sil 5 CB, 30 m x 0.53 mm, 1.50 µm

CP8735

D 3452
D 3465
D 3524
D 3545
D 3606

D 3687

D 3695
D 3710
D 3749

D 3760

D 3792
D 3797
D 3798
D 3871
D 3876

CP-Sil 5 CB, 25 m x 0.32 mm, 0.52 µm
CP-Sil 5 CB, 30 m x 0.53 mm, 1.50 µm
Standard Test Method for Diesel Fuel Diluent in Used CP-SimDist UltiMetal, 10 m x 0.53 mm, 0.53 µm
Diesel Engine Oils by Gas Chromatography
Standard Test Method for Alcohol Content and Purity DB-624, 30 m x 0.53 mm, 3.00 µm
of Acetate Esters by GC
Standard Test Method for Determination of Benzene VF-1ms, 15 m x 0.25 mm, 0.10 µm
and Toluene in Finished Motor and Aviation Gasoline CP-TCEP for Alcohols in Gasoline, 50 m x 0.25 mm,
by Gas Chromatography
0.40 µm
Standard Test Method for Analysis of Organic Vapors DB-WAX, 30 m x 0.53 mm, 1.00 µm
Collected by the Activated Charcoal Tube Adsorption DB-WAX, 30 m x 0.45 mm, 0.85 µm
Method
CP-WAX 52 CB, 30 m x 0.32 mm, 0.50 µm
CP-WAX 52 CB, 30 m x 0.53 mm, 1.00 µm
Standard Test Method for Volatile Alcohols in Water DB-WAX, 30 m x 0.53 mm, 1.00 µm
by Direct Aqueous-Injection GC
CP-SimDist UltiMetal, 10 m x 0.53 mm, 0.53 µm
Standard Test Method for Boiling Range Distribution DB-2887, 10 m x 0.53 mm, 3.00 µm
of Gasoline and Gasoline Fractions by GC
Standard Test Method for Residual Vinyl Chloride
CP-PoraBOND Q, 10 m x 0.32 mm, 5.00 µm
Monomer in Poly(Vinyl Chloride) Resins by Gas
CP-PoraBOND Q, 10 m x 0.53 mm, 10.00 µm
Chromatographic Headspace Technique
Standard Test Method for Analysis of
DB-WAX, 60 m x 0.32 mm, 0.25 µm
Isopropylbenzene (Cumene) by GC
HP-1, 50 m x 0.32 mm, 0.52 µm
CP-Xylenes, 50 m x 0.53 mm,
Standard Test Method for Water Content of Coatings CP-PoraBOND Q, 25 m x 0.32 mm, 5.00 µm
by Direct Injection Into a Gas Chromatograph
CP-PoraBOND Q, 25 m x 0.53 mm, 10.00 µm
Standard Test Method for Analysis of o-Xylene by GC HP-INNOWax, 60 m x 0.32 mm, 0.50 µm
CP-Xylenes, 50 m x 0.53 mm
Standard Test Method for Analysis of p-Xylene by GC HP-INNOWax, 60 m x 0.32 mm, 0.50 µm
CP-Xylenes, 50 m x 0.53 mm
Standard Test Method for Purgeable Organic
DB-VRX, 75 m x 0.45 mm, 2.55 µm
Compounds in Water Using Headspace Sampling
Standard Test Method for Methoxyl and
CP-Sil 5 CB, 30 m x 0.32 mm, 1.00 µm
Hydroxypropyl Substitution in Cellulose Ether
CP-Sil 5 CB, 30 m x 0.53 mm, 1.50 µm
Products by Gas Chromatography

CP8430
CP8735
CP7592
125-1334
CP8906
CP7525
125-7032
124-7032
CP8763
CP8738
125-7032
CP7592
125-2814
CP7350
CP7353
123-7062
19091Z-115
CP7428
CP7351
CP7354
19091N-216
CP7428
19091N-216
CP7428
124-1574
CP8760
CP8735
(Continued)

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553

GC Column Application and Method Guides

ASTM Methods
Method

Title

Recommended Agilent Column

Part No.

D 3893

Standard Test Method for Purity of Methyl Amyl
Ketone and Methyl Isoamyl Ketone by GC
Standard Test Method for Low-Molecular Weight
Halogenated Hydrocarbons in Water
Standard Test Method for Analysis
of Polychlorinated Biphenyls in Insulating Liquids
by Gas Chromatography
Standard Test Method for Determination of
Butylated Hydroxy Toluene (BHT) in Polymers
of Ethylene and Ethylene – Vinyl Acetate (EVA)
Copolymers By Gas Chromatography
Standard Test Method for Residual Acrylonitrile
Monomer Styrene-Acrylonitrile Copolymers and
Nitrile Rubber by Headspace Gas Chromatography
Standard Test Method for Benzene in Hydrocarbon
Solvents by Gas Chromatography

DB-VRX, 30 m x 0.45 mm, 2.55 µm

124-1534

DB-VRX, 30 m x 0.45 mm, 2.55 µm

124-1534

CP-Sil 8 CB for PCB, 50 m x 0.25 mm, 0.25 µm

CP7482

CP-Sil 5 CB, 30 m x 0.32 mm, 3.00 µm
CP-Sil 5 CB, 30 m x 0.53 mm, 3.00 µm

CP8687
CP8677

CP-PoraBOND Q, 25 m x 0.53 mm, 10.00 µm

CP7354

VF-1ms, 15 m x 0.25 mm, 0.10 µm
CP-TCEP for Alcohols in Gasoline, 50 m x 0.25 mm,
0.40 µm
DB-FFAP, 30 m x 0.32 mm, 0.25 µm

CP8906
CP7525

HP PLOT Al2O3 S, 50 m x 0.53 mm, 15 µm
CP-Al2O3/Na2SO4, 25 m x 0.53 mm, 10.00 µm
DB-VRX, 30 m x 0.45 mm, 2.55 µm

19095P-S25
CP7567
124-1534

CP-TCEP for Alcohols in Gasoline, 50 m x 0.25 mm,
0.40 µm
CP-PoraBOND Q, 25 m x 0.32 mm, 5.00 µm
CP-PoraBOND Q, 25 m x 0.53 mm, 10.00 µm

CP7525

CP-TCEP for Alcohols in Gasoline, 50 m x 0.25 mm,
0.40 µm
DB-FFAP, 30 m x 0.45 mm, 0.85 µm
CP-Wax 58 FFAP CB, 25 m x 0.53 mm, 1.00 µm
CP-Wax 58 FFAP CB, 25 m x 0.53 mm, 1.00 µm

CP7525
124-3232
CP7614
CP7614

DB-WAX, 30 m x 0.45 mm, 0.85 µm

124-7032

D 3973
D 4059

D 4275

D 4322

D 4367

D 4415
D 4424
D 4443

D 4492
D 4509

D 4534
D 4735
D 4768

D 4864

Standard Test Method for Determination of Dimer
in Acrylic Acid
Standard Test Method for Butylene Analysis by GC
Standard Test Method for Residual Vinyl Chloride
Monomer Content in PPB Range in Vinyl Chloride
Homo- and Co-Polymers by Headspace GC
Standard Test Method for Analysis of Benzene
by Gas Chromatography
Standard Test Methods for Determining the 24-Hour
Gas (AIR) Space Acetaldehyde Content of Freshly
Blown PET Bottles
Test Method for Benzene Content of Cyclic Products
by Gas Chromatography
Standard Test Method for Determination of Trace
Thiophene in Refined Benzene by GC
Standard Test Method for Analysis of 2,6-DitertiaryButyl Para-Cresol and 2,6-Ditertiary- Butyl Phenol
in Insulating Liquids by Gas Chromatography
Standard Test Method for Determination of Traces
of Methanol in Propylene Concentrates by GC

123-3232

CP7351
CP7354

(Continued)

554

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GC Column Application and Method Guides

ASTM Methods
Method

Title

Recommended Agilent Column

Part No.

D 4947

Standard Test Method for Chlordane and Heptachlor
Residues in Indoor Air

D 4961

Standard Test Method for GC Analysis of Major
Organic Impurities in Phenol Produced by the
Cumene Process
Standard Test Method for Cyclohexylamine
Morpholine and Diethylaminoethanol in Water and
Condensed Steam by Direct Aqueous Injection GC
Standard Test Method for Ethyl Methyl Pentonal
Content and Purity Value of 2-Ethylhexanol by GC

DB-5, 30 m x 0.53 mm, 1.50 µm
DB-608, 30 m x 0.53 mm, 0.83 µm
DB-FFAP, 30 m x 0.45 mm, 0.85 µm
HP PLOT Q, 15 m x 0.53 mm, 40 µm

125-5032
125-1730
124-3232
19095P-QO3

HP-5ms, 30 m x 0.32 mm, 1.00 µm
CAM, 30 m x 0.53 mm, 1 µm

19091S-213
115-2132

HP-1, 15 m x 0.53 mm, 5.00 µm
HP-INNOWax, 30 m x 0.32 mm, 0.25 µm
HP-INNOWax, 60 m x 0.32 mm, 0.50 µm
CP-WAX 52 CB, 60 m x 0.32 mm, 0.50 µm
DB-5, 30 m x 0.53 mm, 1.50 µm
DB-5, 30 m x 0.32 mm, 1.00 µm
HP-PONA, 50 m x 0.20 mm, 0.50 µm
CP-Sil PONA for ASTM D 5134, 50 m x 0.21 mm,
0.50 µm
HP-INNOWax, 60 m x 0.32 mm, 0.50 µm
CP-WAX 52 CB, 60 m x 0.32 mm, 0.50 µm
DB-1, 30 m x 0.32 mm, 1.00 µm
DB-608, 30 m x 0.32 mm, 0.5 µm
DB-XLB, 30 m x 0.25 mm, 0.25 µm
GS-GasPro, 30 m x 0.32 mm
HP PLOT Q, 30 m x 0.53 mm, 40 µm
HP-1, 7.5 m x 0.53 mm, 5.00 µm

19095Z-621
19091N-113
19091N-216
CP8773
125-5032
123-5033
19091S-001
CP7531

HP-5ms, 30 m x 0.25 mm, 0.25 µm
DB-225ms, 30 m x 0.25 mm, 0.25 µm
HP-1ms, 30 m x 0.32 mm, 1.00 µm
DB-624, 30 m x 0.45 mm, 2.55 µm

19091S-433
122-2932
19091S-713
124-1334

HP-5ms, 30 m x 0.25 mm, 0.25 µm
DB-1701P, 30 m x 0.25 mm, 0.25 µm
DB-XLB, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.25 mm, 0.25 µm

19091S-433
122-7732
122-1232
122-3832

D 4983

D 5008
D 5060

Standard Test Method for Determining Impurities
in High-Purity Ethylbenzene by GC

D 5075

Standard Test Method for Nicotine in Indoor Air

D 5134

Standard Test Method for Detailed Analysis
of Petroleum Naphthas Through n-Nonane
by Capillary GC

D 5135

Standard Test Method for Analysis of Styrene
by Capillary GC

D 5175

Standard Test Method for Organohalide Pesticides
and Polychlorinated Biphenyls in Water by
Microextraction and GC

D 5303

Standard Test Method for Trace Carbonyl Sulfide
in Propylene by GC

D 5307

Standard Test Method for Determination of Boiling
Range Distribution of Crude Petroleum by GC
Standard Test Method for Tar Acid Composition by
Capillary GC

D 5310
D 5316

D 5317

Standard Test Method for 1, 2-Dibromoethane
and 1, 2-Dibromo-3-Chloropropane in Water by
Microextraction and GC
Standard Test Method for Determination of
Chlorinated Organic Acid Compounds in Water
by GC with Electron Capture Detector

19091N-216
CP8773
123-1033
123-1730
122-1232
113-4332
19095P-QO4
19095Z-627

(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

555

GC Column Application and Method Guides

ASTM Methods
Method

Title

Recommended Agilent Column

Part No.

D 5320

Standard Test Method for Determination of
1, 1-Trichloroethane and Methylene Chloride in
Stabilized Trichloroethylene and Tetrachloroethylene
Standard Test Method for Boiling Point Distribution
of Hydrocarbon Solvents by GC
Standard Test Method for Analysis of
Methyl Tert-Butyl Ether (MTBD) by GC

DB-1, 30 m x 0.53 mm, 3.00 µm
DB-VRX, 30 m x 0.32 mm, 1.80 µm

125-1034
123-1534

DB-2887, 10 m x 0.53 mm, 3.00 µm

125-2814

HP-PONA, 50 m x 0.20 mm, 0.50 µm
DB-Petro, 100 m x 0.25 mm, 0.50 µm
DB-1, 25 m x 0.32 mm, 0.25 µm
DB-5, 15 m x 0.25 mm, 0.25 µm
HP-5ms, 30 m x 0.25 mm, 0.25 µm
DB-1701P, 30 m x 0.25 mm, 0.25 µm
DB-XLB, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.25 mm, 0.25 µm
DB-PS1, 15 m x 0.53 mm, 0.15 µm
HP-1, 100 m x 0.25 mm, 0.50 µm

19091S-001
122-10A6
123-1022
122-5012
19091S-433
122-7732
122-1232
122-3832
145-1011
19091Z-530

CP-Sil 5 CB for Sulfur, 30 m x 0.32 mm, 4.00 µm

CP7529

HP PLOT Q, 15 m x 0.53 mm, 40 µm
HP PLOT U, 30 m x 0.53 mm, 20 µm

19095P-QO3
19095P-UO4

HP PLOT Q, 30 m x 0.53 mm, 40 µm

19095P-QO4

DB-1, 30 m x 0.53 mm, 5.00 µm
CP-TCEP for Alcohols in Gasoline, 50 m x 0.25 mm,
0.40 µm
CP-Sil 5 CB, 30 m x 0.53 mm, 5.00 µm
VF-1ms, 15 m x 0.25 mm, 0.10 µm
DB-5, 30 m x 0.25 mm, 0.25 µm

125-1035
CP7525

D 5399
D 5441
D 5442

Standard Test Method for Analysis of Petroleum
Waxes by GC

D 5475

Standard Test Method for Nitrogen- and
Phosphorus-Containing Pesticides in Water
by GC with a Nitrogen Phosphorus Detector

D 5480
D 5501

Standard Test Method for Engine Oil Volatility by GC
Standard Test Method for Determination of Ethanol
Content of Denatured Fuel Ethanol by GC
Standard Test Method for Determination of Sulfur
Compounds in Natural Gas and Gaseous Fuels
by Gas Chromatography and Chemiluminescence
Standard Test Method for Determination of Trace
Organic Impurities in Monomer Grade Vinyl Chloride
by Capillary Column/Multi-dimensional GC
Standard Test Method for Determination of Residual
Acrylonitrile Monomer in Styrene-Acyrlonitrile
Co-polymer Resins and Nitrile-Butadiene Rubber
by Headspace Capillary GC
Standard Test Method for Determination of Benzene,
Toluene, Ethylbenzene, p/m-Xylene, C9 and Heavier
Aromatics, and Total Aromatics in Finished Gasoline
by GC

D 5504

D 5507

D 5508

D 5580

D 5599

Standard Test Method for Determination of
Oxygenates in Gasoline by GC and Oxygen Selective
Flame Ionization Detection

CP8775
CP8906
122-5032

(Continued)

556

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GC Column Application and Method Guides

ASTM Methods
Method

Title

Recommended Agilent Column

Part No.

D 5623

Standard Test Method for Sulfur Compounds
in Light Petroleum Liquids by GC and Sulfur
Selective Detection
Standard Test Method for Analysis of High Purity
Benzene for Cyclohexane Feedstock by Capillary GC
Standard Practice for Oil Spill Source Identification
by GC and Positive Ion Electron Impact Low
Resolution Mass Spectrometry
Standard Test Method for Determination of Benzene,
Toluene, and Total Aromatics in Finished Gasoline
by GC/MS
Standard Test Method for Measurement
of Purgeable Organic Compounds in Water
by Capillary Column GC/MS

HP-1, 30 m x 0.32 mm, 4.00 µm

19091Z-613

DB-Petro, 50 m x 0.20 mm, 0.5 µm

122-10A6E

DB-5, 30 m x 0.25 mm, 0.25 µm
DB-TPH, 30 m x 0.32 mm, 0.25 µm

122-5032
123-1632

HP-1, 60 m x 0.25 mm, 1.00 µm

19091Z-236

DB-VRX, 60 m x 0.25 mm, 1.40 µm
DB-VRX, 20 m x 0.18 mm, 1.00 µm
DB-624, 60 m x 0.25 mm, 1.4 µm
DB-624, 20 m x 0.18 mm, 1.00 µm
HP-5MS, 30 m x 0.25 mm, 0.25 µm
DB-1701P, 30 m x 0.25 mm, 0.25 µm
DB-XLB, 30 m x 0.25 mm, 0.25 µm
DB-35ms, 30 m x 0.25 mm, 0.25 µm
HP-INNOWax, 60 m x 0.32 mm, 0.25 µm

122-1564
121-1524
122-1364
121-1324
19091S-433
122-7732
122-1232
122-3832
19091N-116

DB-23, 60 m x 0.25 mm, 0.25 µm

122-2362

HP-1, 60 m x 0.53 mm, 5.00 µm

19095Z-626

HP-1, 60 m x 0.25 mm, 1.00 µm

19091Z-236

HP PLOT Al2O3 "KCl", 50 m x 0.53 mm, 15 µm
GS-Alumina, 50 m x 0.53 mm
DB-1, 30 m x 0.53 mm, 5.00 µm
HP-5MS, 30 m x 0.32 mm, 0.25 µm
DB-XLB, 30 m x 0.25 mm, 0.25 µm
DB-HT SimDis, 5 m x 0.53 mm, 0.15 µm

19095P-K25
115-3552
125-1035
19091S-413
122-1232
145-1001

D 5713
D 5739

D 5769

D 5790

D 5812

Standard Test Method for Determination
of Organochlorine Pesticides in Water
by Capillary Column GC

D 5917

Standard Test Method for Trace Impurities
in Monocyclic Aromatic Hydrocarbons by GC
and External Calibration
Standard Test Method for Fatty and Rosin Acids
in Tall Oil Fraction Products by Capillary GC
Standard Test Method for Determination of
Oxygenates, Benzene, Toluene, C8-C12 Aromatics
and Total Aromatics in Finished Gasoline by GC/FTIR
Standard Test Method for Trace Impurities in
Alpha-Methylstyrene by Capillary GC
Standard Test Method for Determination of
Hydrocarbon Impurities in Ethylene by GC

D 5974
D 5986

D 6144
D 6159

D 6160

Standard Test Method for Determination of PCBs
in Waste Materials by GC

D 6352

Standard Test Method for Boiling Range Distribution
of Petroleum Distillates in Boiling Range from
174 to 700 by GC

(Continued)

AGILENT J&W GC AND GC/MS COLUMNS

557

GC Column Application and Method Guides

ASTM Methods
Method

Title

Recommended Agilent Column

Part No.

D 6387

Standard Test Methods for Composition of Turpentine
and Related Terpene Products by Capillary Gas
Chromatography
Standard Test Method for Estimation of Engine Oil
Volatility by Capillary GC
Standard Test Method for Determination of Total
Monoglyceride, Total Diglyceride, Total Triglyceride,
and Free and Total Glycerin in B-100 Biodiesel
Methyl Esters by Gas Chromatography
Standard Practice for Analysis of Halogenated
Organic Solvents and Their Admixtures
by Gas Chromatography
Standard Test Method for Analysis of Acetic
Anhydride Using GC
Standard Test Method for Analysis of Acrylonitrile
by GC
Standard Test Method for Analysis of Ethylene
Glycols and Propylene Glycols

CP-WAX 52 CB, 30 m x 0.32 mm, 0.50 µm
CP-WAX 52 CB, 30 m x 0.53 mm, 1.00 µm

CP8763
CP8738

DB-HT SimDis, 5 m x 0.53 mm, 0.15 µm

145-1001

Select Biodiesel, 15 m x 0.32 mm, 0.10 µm

CP9078

CP-Sil 5 CB, 50 m x 0.53 mm, 5.00 µm

CP7685

HP-1, 50 m x 0.32 mm, 0.52 µm

19091Z-115

DB-WAXetr, 60 m x 0.32 mm, 1.00 µm

123-7364

DB-624, 30 m x 0.53 mm, 3.00 µm
CP-Wax 57 CB for Gycols and Alcohols, 50 m x 0.25
mm, 0.25 µm
HP-5, 30 m x 0.53 mm, 5.00 µm

125-1334
CP7615

D 6417
D 6584

D 6806

E 1616
E 1863
E 0202

E 0475

Standard Test Method for Assay of Di-tert-Butyl
Peroxide Using GC

Tips & Tools
GC Method Translation Software allows you to port a current
GC method to another while ensuring that relative retention order
is maintained so peaks elute in the same order. Download at
www.agilent.com/chem/gcmethodtranslation

558

GC AND GC/MS

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19095J-623

GC Column Troubleshooting and Maintenance

GC Column Troubleshooting
and Maintenance
These at-a-glance troubleshooting tables will help you pinpoint and fix the most common
GC column problems.
Please consult Agilent's J&W GC Column Installation Guide (publication number 830-0120)
and the Agilent J&W GC Column Selection Guide (publication number 5989-6159EN)
for more in-depth information, including:

• Comprehensive column selection principles
• Maintenance procedures, including column installation, conditioning, testing, and storage
• Strategies for increasing your productivity
• The most current method development procedures

To request your copy of the Agilent J&W GC Column Selection Guide, visit
www.agilent.com/chem/guides
The Agilent J&W GC Column Installation Guide comes standard with every Agilent
J&W GC column. You can also request a copy by contacting your local Agilent
Representative or Agilent Authorized Distributor.

AGILENT J&W GC AND GC/MS COLUMNS

559

GC Column Troubleshooting and Maintenance

Excessive Baseline Noise
Possible Cause
Injector contamination

Solution
Clean the injector; replace liner, gold seal

Column contamination

Bake-out the column
Solvent rinse the column

Detector contamination

Clean the detector

Contaminated or low quality gases

Use better grade gases; also check for expired
gas traps or leaks
Reinstall the column

Column inserted too far into the detector
Incorrect detector gas flow rates
Leak when using an MS, ECD, or TCD
Old detector filament, lamp
or electron multiplier
Septum degradation

560

GC AND GC/MS

Adjust the flow rates to the
recommended values
Find and eliminate the leak
Replace appropriate part
Replace septum

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Comments
Try a condensation test; gas lines may also
need cleaning
Limit the bake-out to 1-2 hours
Only for bonded and cross-linked phases
Check for inlet contamination
Usually the noise increases over time
and not suddenly
Usually occurs after changing a gas cylinder
Consult GC manual for proper
insertion distance
Consult GC manual for proper flow rates
Usually at the column fittings or injector

For high temperature applications use
an appropriate septum

GC Column Troubleshooting and Maintenance

Baseline Instability or Disturbances
Possible Cause
Injector contamination

Solution
Clean the injector

Unequilibrated detector

Allow the detector to stabilize

Incompletely conditioned column
Change in carrier gas flow rate during
the temperature program
Column contamination

Fully condition the column
Normal in many cases
Trim the column
Solvent rinse the column

Column activity
Solvent-phase polarity mismatch

Irreversible. Replace the column
Change sample solvent to a single solvent
Use a retention gap
Decrease the initial column temperature

Solvent effect violation for splitless or
on-column injections
Too low of a split ratio

Increase the split ratio

Poor column installation
Some active compounds always tail

Reinstall the column
None

Comments
Try a condensation test; gas lines may also
need cleaning
Some detectors may require up to 24 hours
to fully stabilize
More critical for trace level analyses
MS, TCD and ECD respond to changes in carrier
gas flow rate
Remove 0.5-1 m from the front of the column
Only for bonded and cross-linked phases
Check for inlet contamination
Only affects active compounds
More tailing for the early eluting peaks or those
closest to the solvent front
3-5 m retention gap is sufficient
Peak tailing decreases with retention
Flow from split vent should be 20 mL/min
or higher
More tailing for early eluting peaks
Most common for amines and carboxylic acids

Split Peaks
Possible Cause
Injection technique

Solution
Change technique

Mixed sample solvent

Change sample solvent to a single solvent

Poor column installation
Sample degradation in the injector

Reinstall the column
Reduce the injector temperature

Poor sample focusing

Change to an on-column injection
Use a retention gap

Comments
Usually related to erratic plunger depression
or having sample in the syringe needle.
Use an auto injector.
Worse for solvents with large differences in
polarity or boiling points
Usually a large error in the insertion distance
Peak broadening or tailing may occur if
the temperature is too low
Requires an on-column injector
For splitless and on-column injection

AGILENT J&W GC AND GC/MS COLUMNS

561

GC Column Troubleshooting and Maintenance

Retention Time Shift
Possible Cause
Change in carrier gas velocity

Solution
Check the carrier gas velocity

Change in column temperature
Change in column dimension
Large change in compound concentration

Check the column temperature
Verify column identity
Try a different sample concentration

Leak in the injector
Blockage in a gas line

Leak check the injector
Clean or replace the plugged line

Septum leak
Sample solvent incompatibility

Replace septum
Change sample solvent to a single solvent
Use a retention gap

Comments
All peaks will shift in the same direction by
approximately the same amount
Not all peaks will shift by the same amount
May also affect adjacent peaks. Sample
overloading is corrected with an increase in
split ratio or sample dilution.
A change in peak size usually occurs
More common for the split line; also check
flow controllers and solenoids
Check for needle barb
For splitless injection

Change in Peak Size
Possible Cause
Change in detector response

Solution
Check gas flows, temperatures and settings
Check background level or noise

Change in the split ratio
Change in the purge activation time
Change in injection volume
Change in sample concentration

Check split ratio
Check the purge activation line
Check the injection technique
Check and verify sample concentration

Leak in the syringe

Use a different syringe

Column contamination

Trim the column

Column activity
Coelution
Change in injector discrimination
Sample flashback
Decomposition from inlet contamination

562

GC AND GC/MS

Solvent rinse the column
Irreversible
Change column temperature
or stationary phase
Maintain the same injector parameters
Inject less, use a larger liner, reduce the
inlet temperature
Clean the injector; replace liner, gold seal

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Comments
All peaks may not be equally affected
May be caused by system contamination
and not the detector
All peaks may not be equally affected
For splitless injection
Injection volumes are not linear
Changes may also be caused by degradation,
evaporation, or variances in sample
temperature or pH
Sample leaks past the plunger or around the
needle; leaks are not often readily visible
Remove 0.5-1 m from the front of the column
Only for bonded and cross-linked phases
Only affects active compounds
Decrease column temperature and check
for the appearance of a peak shoulder or tail
Most severe for split injections
Less solvent and higher flow rates are
most helpful
Only use deactivated liners and glass wool
in the inlet

GC Column Troubleshooting and Maintenance

Loss of Resolution
Possible Cause

Solution

Comments

Check the column temperature
Verify column identity
Change column temperature

Differences in other peaks will be visible
Differences in other peaks will be visible
Decrease column temperature and check
for the appearance of a peak shoulder or tail

Change in carrier gas velocity
Column contamination

Check the carrier gas velocity
Trim the column

A change in the retention time also occurs
Remove 0.5-1 m from the front of the column

Change in the injector

Solvent rinse the column
Check the injector settings

Only for bonded and cross-linked phases
Typical areas: split ratio, liner, temperature,
injection volume
Peak widths increase at higher concentrations
For splitless injection

Decrease in separation

Different column temperature
Different column dimensions or phase
Coelution with another peak
Increase in peak width

Change in sample concentration
Improper solvent effect, lack of focusing

Try a different sample concentration
Lower oven temperature, better solvent,
sample phase polarity match, use a
retention gap

Tips & Tools
Watch Agilent's in-depth video series cover common
chromatographic problems, causes, and corresponding
solutions presented by two of Agilent's seasoned GC experts
at www.agilent.com/chem/gctroubleshooting

AGILENT J&W GC AND GC/MS COLUMNS

563

LC AND LC/MS

710

LC AND LC/MS

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l 01357 522 961

In this Chapter
Parts and Supplies
712
716
739
752
758
762
772
775
787

Bio-inert Supplies
General Supplies
Pump Supplies
Autosampler Supplies
Fraction Collector Supplies
Valve Supplies
TCC Supplies
Detector Supplies
LC/MS Supplies

LC and LC/MS Columns
796
806
818
891
916
945
996

HPLC Column Selection
Cartridge Column Systems
Columns for Analytical HPLC
Columns for Special Applications
Preparative HPLC Columns and Flash Chromatography
GPC/SEC Columns and Calibrants
Biosolutions and Columns for Biologic Characterization

LC and LC/MS Troubleshooting
1052 HPLC Troubleshooting
1056 LC/MS Troubleshooting

LC and LC/MS Applications
1059
1076
1079
1088
1102

BioPharmaceutical Applications
Chemical/Industrial Applications
Environmental Applications
Food and Consumer Product Applications
Pharmaceutical Applications

LC AND LC/MS

711

LC/MS Supplies

LC/MS Supplies
Combined with Agilent's industry-leading LC systems, our single quadrupole, ion trap,
triple quadrupole, TOF and Q-TOF LC/MS solutions combine world-class performance
with legendary reliability and ease-of-use.
This section contains all of the mass spectrometry supplies you need to keep your
LC mass spectrometer running at peak performance.

LC/MS Maintenance Schedule
Procedure
Flush the nebulizer
Clean the electrospray
spray chamber
Replace the electrospray
nebulizer needle
Clean the APCI spray
chamber
Replace the APCI
nebulizer needle
Clean the multimode source

Check calibrant levels

When to Perform
Daily or at the end of each shift to flush traces of samples
and buffers out of the tubing, valves, and nebulizer.
Daily or anytime you suspect carryover contamination from
one sample or analysis to another.
When the needle is plugged. Common symptoms of a plugged
needle are increased LC back pressure, off-axis spraying,
or dripping from the nebulizer.
Daily or anytime you suspect carryover contamination from
one sample or analysis to another.
When the needle is plugged. Common symptoms of a plugged
needle are increased LC back pressure or off-axis spray from
the nebulizer.
Daily or anytime you suspect carryover contamination from one
sample or analysis to another, or when you must access the end
cap and capillary cap for cleaning and inspection.
Monthly or weekly if you tune the LC/MS frequently.

PARTS AND SUPPLIES

787

LC/MS Supplies

LC/MS Preventive Maintenance Kit
For your convenience, the LC/MS Preventive Maintenance Kit has the recommended
common supplies needed for most Agilent LC/MS systems. Unique source parts should
be ordered separately.

LC/MS Preventive Maintenance kit
Description
LC/MS Preventive Maintenance kit
Foreline pump oil, Inland 45, 1 L, for E1M18/E2M28
Oil mist filter element for E1M18/E2M28
Filter element, 5 µm, 5/pk
Spring, canted coil, 4/pk
Big hydrocarbon trap, 1/4 in. fittings
Rotor seal, Vespel, pH 0 to 10

Tips & Tools
Save ordering time and money with the LC/MS PM Kit!
It contains the common supplies specified in Agilent service
engineer preventive maintenance procedure for LC/MS platforms.

788

LC AND LC/MS

www.agilent.com/chem/LC
www.crawfordscientific.com

l 01357 522 961

Part No.
5190-1443
6040-0834
1535-4970
0100-2051
1460-2571
BHT-4
0100-1855

LC/MS Supplies

LC/MS Supplies

Description
ES nebulizer assembly, original
ES nebulizer needle (original) replacement kit
ES nebulizer assembly, new
ES nebulizer needle (new) replacement kit
APCI nebulizer assembly
APCI nebulizer needle replacement kit
Needle assembly APCI/Multimode
Corona needle APCI/Multimode
Capillary cap, high temperature, 3.0 mm
Capillary, 0.5 mm ID, dielectric*
Capillary, 0.6 mm ID, dielectric*
Capillary, 0.6 mm ID, resistive, fast polarity switching*
Spring, canted coil, 0.25 in. ID, 0.53 mm
1/6 in. tee, low dead volume, stainless steel

6100 Series
Single
Quadropole
LC/MS
]
]
]
]
]
]
]
]
]
]
]

6200
Series
TOF
LC/MS
]
]
]
]
]
]
]
]
]

]

]
]

]

6300
Series
Ion Trap
LC/MS
]
]
]
]
]
]
]
]
]
]
]
]

6400 Series 6500 Series
Triple
AccurateQuadropole Mass Q-TOF
LC/MS
LC/MS
Part No.
]
]
G1946-60098
]
]
G2427A
]
]
G1958-60098
]
]
G1958-60136
]
]
G1946-60037
]
]
G2428A
]
G1947-60103
]
]
G1947-20029
]
]
G1946-20301
]
]
]

]
]
]

G1946-80009
59987-20040
G1960-80060
1460-2571
0100-0969

*Dielectric capillary supports standard polarity switching only. Resistive capillary supports fast polarity switching

(Continued)

Tips & Tools
ES nebulizer (original) is compatible with the following ion sources:
Corona needle APCI, G1947-20029

• ESI G1948A with Serial Number < US91801994
• ESI G1948B with Serial Number < US91201787
• Multimode G1978A with Serial Number < US90800804
• Multimode G1978B with Serial Number < US90700787
• Dual ESI G3251A with Serial Number < US91200355
• Dual ESI G3251B with Serial Number < US91200355

PARTS AND SUPPLIES

789

LC/MS Supplies

LC/MS Supplies

Description
Syringe adapter
Syringe pump
1/16 in. finger-tight PEEK fitting
Female luer to female 10/32 adapter
PEEK tubing
Gas-tight syringe, PTFE luer lock
High-throughput skimmer, 2 mm
Skimmer 1 (G1956A/B)
HED assembly
HED assembly (G6140A, G6460A, G6530A)
HED assembly
Electron multiplier replacement horn
Replacement horn and dynode

6100 Series
Single
Quadropole
LC/MS

6200
Series
TOF
LC/MS

]
]
]
]
]

6300
Series
Ion Trap
LC/MS
]
]
]
]
]
]

]
]

]

]
]

]

]

*Dielectric capillary supports standard polarity switching only. Resistive capillary supports fast polarity switching

05971-80103

790

LC AND LC/MS

www.agilent.com/chem/LC
www.crawfordscientific.com

l 01357 522 961

6400 Series 6500 Series
Triple
AccurateQuadropole Mass Q-TOF
LC/MS
LC/MS
Part No.
9301-1291
3162-0178
]
0100-1516
]
0100-2304
]
0890-1915
]
5182-9710
]
]
G1969-20302
G1956-20302
G1946-80019
]
G2571-80103
G1956-80000
]
05971-80103
]
G2441-80010

LC/MS Supplies

LC/MS Foreline Pump Supplies
Description
Oil mist filter kit for E1M18/E2M28
Oil mist cartridge filter for MS40+
Oil return kit
Foreline pump oil, Inland 45, 1 L, for E1M18/E2M28
Rotary pump oil, 4 L, for E1M18/E2M28
Foreline exhaust adapter
Hose clamp
Oil mist filter element for E1M18/E2M28
KF25 clamp, stainless steel
KF25 coseal (inside clamp)
Exhaust tubing
Pump oil drip pan

Part No.
3162-1056
G1960-80039
3162-1057
6040-0834
6040-0798
59980-20134
1400-0563
1535-4970
0100-0549
0100-1597
0890-1727
G1946-00034

LC/MS Chemicals

Description
ES/APCI positive ion performance standard,
5 x 1 mL ampoules
Electrospray LC demo sample (Sulfamix)
ESI+APCI LC demo sample
ES negative ion performance standard,
5 x 1 mL ampoules
APCI negative ion performance standard,
5 x 1 mL ampoules
ES/APCI positive ion performance standard
Multiple-charge compound performance evaluation
sample (horse heart myoglobin)
Caffeine standards kit for LC/MS OQ/PV
ES/APCI positive ion performance standard,
5 x 1 mL ampoules
ES-TOF reference mix, 6 x 2 mL ampoules
ES-TOF biopolymer reference standard kit
Flushing solvent
High purity water, 4 L
Methyl alcohol, 1 L
Ammonium formate
Formic acid, 5 mL
Acetonitrile, 1 L

6100 Series
6400 Series 6500 Series
Single
6300 Series
Triple
AccurateQuadropole 6200 Series Ion Trap Quadropole Mass Q-TOF
LC/MS TOF LC/MS LC/MS
LC/MS
LC/MS
Part No.
]

]

]

]

G2423A

]
]

]*

59987-20033
G1978-85000

]

]

G2424A

]

G2425A

]

G1946-85004

]

G2426A

]

8500-6917

]

G2423A

]
]
]
]
]
]

]
]
]
]
]
]
]
]

]
]
]
]
]
]
]

]
]
]
]
]
]

]
]
]
]
]
]

G1969-85001
G1969-85003
G1969-85026
8500-2236
8500-1867
G1946-85021
G2453-85060
G2453-85050

*Recommended item for familiarization

PARTS AND SUPPLIES

791

LC/MS Supplies

LC/MS Common Supplies*
Description

Part No.

Common Parts

Filter element, 5 µm, 5/pk
Rotor seal, Tefzel, pH 0 to 14
Rotor seal, Vespel, pH 0 to 10
Inlet filter assembly
SSV long drain tubing assembly
Spring, canted coil

0100-2051
0100-1854
0100-1855
G1946-60180
G1969-60086
1460-2571

Cleaning Supplies

Abrasive mesh, 4000 grit
Capillary cleaning wire for dip tube
Cleaning powder, dielectric capillary, Alconox
Cloths, lint-free
Cotton swabs, 100/pk

8660-0827
G1946-80054
5190-1401
05980-60051
5080-5400

Gas Purifiers

Big hydrocarbon trap, 1/4 in. fittings
Big moisture trap, 1/4 in. fittings
Big universal trap, 1/4 in. fittings
Big universal trap, 1/8 in. fittings, Nitrogen

BHT-4
BMT-4
RMSN-4
RMSN-2

Tools

LC/MS tool kit
Nebulizer adjustment fixture
Nebulizer 25X magnifier
Plastic tubing cutter
Screwdriver, Torx T15
Screwdriver, Torx T20
Open end wrench, 1/4 and 5/16 in.
Wrench, 1/2 and 7/16 in.
Needle nose pliers, pointed serrated jaws
3 mm wrench for nebulizer needle adjustment
*These parts are common to all LC/MS systems

Tips & Tools
More information is a click away. We have a variety
of educational primers, application notes, maintenance
guides, and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

792

LC AND LC/MS

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www.crawfordscientific.com

l 01357 522 961

G1946-60157
G1946-20215
G1946-80049
8710-1930
8710-1622
8710-1615
8710-0510
8710-0806
8710-0004
8710-2699

LC/MS Supplies

Quiet Cover
Agilent has a solution to the frequent maintenance and inherent noise of LC/MS rough pumps.
The Quiet Cover II was designed for easy movement, maintenance, and better living with rough
pumps used with Agilent and other LC/MS systems.

• Locking castors to move heavy pump for maintenance
• No tools necessary to remove sectioned cover for easy access to pump
• Built in lift-and-tilt lever raises end of pump to drain oil
• Removable drip pan with well and hand holds to collect and transport oil
• Sound absorbing cabinet with resistant foam insulation to reduce pump noise
• Pump mounted on cushioned grommets to minimize vibration
• 2 Integrated fans maintain temperature inside cover
• LEDs and audible alarm if temperature exceeds 35°C limit
• Maximum ambient temperature of 35°C when airflow is neither restricted nor recycled
• Standard one-year warranty; installation and familiarization included with new LC/MS orders
The Quiet Cover II is compatible with these Agilent LC/MS Systems that use Edwards pumps:

• 6300 Traps: G2440DA, G2451AA, G4533AA, G2474SS
• 6410 QQQ: G6410AA
• 6210 TOF: G3250AA, G3252A
• 6510AA Q-TOF: G6510AA
• Any analytical system using BOC Edwards pumps (lbs/kg): E2M28, E2M18, E1M18
Quiet Cover

Please confirm rough pump used in your Agilent LC/MS system to ensure compatibility.

Quiet Cover II for Agilent LC/MS Systems
Description
Quiet Cover II for Agilent LC/MS Systems
12.5 W x 17.3 H x 33.5 L

Part No.
G3199B

PARTS AND SUPPLIES

793

LC/MS Supplies

LC/MS Standards Kits
LC/MS Standards Kits
Description
Caffeine standards kit for LC/MS OQ/PV
Includes 5 ampoules, 5 mL each: 0.5, 1.0, 5.0, 25.0, and 50.0 µg/mL in water
Caffeine standards kit for LC/MS-Trap OQ/PV
Includes 5 ampoules, 5 mL each: 0.1, 0.5, 1.0, 5.0, 10.0, µg/mL caffeine in water
Sulfa drug standards kit for LC/MS OQ/PV
5 x 2 mL ampoules with 4 sulfa drugs in water/methanol 70:30

Part No.
8500-6917
5065-9908
5188-6523

LC/MS Analyzer Kit Standards
Description
LC/MS toxicology calibration mixture, 3 x 1 mL ampoules
Method EN12916/IP391 LC standard calibration kit, 4 x 1 mL ampoules
Method EN12916/IP391 system calibration standard kit, 2 x 1 mL ampoules
LC TOF/QTOF/QQQ pesticide test mixture, 2 solutions, 3 x 1 mL ampoules of each

Part No.
5190-0470
5190-0484
5190-0485
5190-0469

LC/MS Calibrant Mixes
Description
ESI tuning mix, 100 mL
APCI/APPI calibrant solution, 100 mL
ESI tuning mix for ion trap, 100 mL
ES-TOF tuning mix, 100 mL
APCI-L low concentration tuning mix, 100 mL
MMI-L low concentration tuning mix, 100 mL

Tips & Tools
Agilent offers a variety of e-Seminars and on-site training to help
you learn how to be a more effective chromatographer.
For more information, visit www.agilent.com/chem/education

794

LC AND LC/MS

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www.crawfordscientific.com

l 01357 522 961

Part No.
G2421A
G2432A
G2431A
G1969-85000
G1969-85010
G1969-85020

LC/MS Supplies

LC/MS Calibrant Mix and Source Compatibility Matrix

Source
ESI
APCI
APPI
MMI
NanoESI
HPLC chip cube

6100 Series
Single
Quadropole
LC/MS*
G2421A
G2432A
G2432A
G2432A

6140A/6150B
Single
Quadropole
LC/MS
G1969-85000
G1969-85010
G2432A
G1969-85000
G1969-850002

6200 Series
TOF
LC/MS
G1969-85000
G1969-85010
G1969-85010
G1969-85020
G1969-850004
G1969-850004

6300 Series
Ion Trap
LC/MS
G2431A
G2432A
G2432A
G2432A
G2431A
G2431A1

6400 Series Triple
Quadropole
LC/MS
G1969-85000
G1969-850102
G2432A2
G1969-85020
G1969-850003

6500 Series
Accurate-Mass
Q-TOF LC/MS
G1969-85000
G1969-850102
G1969-850102
G1969-85020
G1969-850004
G1969-850004

*G6110A, G6120A/B, G6130A/B
15X

dilution suggested

2No

autotune

3ESI

positive tune only

4Calibration

only

PARTS AND SUPPLIES

795

HPLC Column Selection Flow Chart

LC and LC/MS Columns
HPLC Column Selection
To use the column selection guide diagram below, simply follow the path for your analyte and mobile phase. At the far right, follow your final
column selection to the pages indicated.

Hexane Soluble

Organic Solvent
Soluble

MeOH and MeOH:
H2O or ACN
and ACN:
H2O Soluble

THF Soluble

Normal-Phase Mode with Bare Silica

see pages
873-874

Normal-Phase Mode with Bonded Phase

see pages
873-874

Reversed-Phase Mode with Bonded Phase

Gel-Permeation (Small Molecule)

see page 827

see pages
947-948

Molecular Weight
<2000
Non-Ionic

Water Soluble

Reversed-Phase Mode with Bonded Phase

see page 827

Reversed-Phase Mode with Bonded Phase
Using Ionization Control

see page 819

Reversed-Phase Mode with Bonded Phase
Using Ion-Pair Agent

see page 819

Reversed-Phase Mode with Bare Silica
(HILIC)

see page 877

Ion-Exchange Mode

see page 879

Ionic

Sample

Organic Solvent
Soluble
Molecular Weight
>2000

Water Soluble

Gel-Permeation Chromatography

Gel-Filtration Chromatography

see page 997

Ion-Exchange Mode
with Wide-Pore Material

see page 1013

Reversed-Phase Mode
with Wide-Pore Material

see page 1016

Adapted with permission from "Practical HPLC Methodology and Applications," Brian A. Bidlingmeyer, John Wiley & Sons, Inc., New York, p. 109

796

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

see pages
947-948

l 01357 522 961

LC and LC/MS Columns

Column and Mobile Phase Guidelines:
Reversed Phase
HPLC columns consist of two parts: the column chemistry and hardware. For the proper column
chemistry, consult the catalog section for each type of bonded phase. For choosing column
hardware and particle sizes, consult the section on column sizes and rapid separations, including
Agilent ZORBAX Rapid Resolution HT, Solvent Saver, Capillary and PrepHT columns.

Pore Size Selection
Choose a column packing with small pore (60-120Å) if the solute molecular weight is less than
about 5000. Otherwise, use column packing with the 300Å pore size.

Particle Size Selection
The typical particle size for HPLC columns is 5 µm with 3.5 µm and smaller now common in
method development. If high-speed analyses or higher resolution analyses are required, packing
with 1.8 µm and 2-3 µm particles can be used. Shorter columns with these particles can produce
faster high-resolution separations, with the 1.8 µm particle size providing the highest efficiency
and 2.7 µm superficially porous providing similar results. With 1.8, 2.7, 3.5 and 5 µm particle
sizes to choose from, start with the smallest particle size for your HPLC or UHPLC – 400 bar, 600
bar, or 1200 bar – to achieve the best results.

Column Configuration
Choosing the best column size for method development has changed dramatically in the past few
years. Smaller 3.0 mm ID or 2.1 mm ID columns are now used more than 4.6 mm ID to lower
solvent use and achieve compatibility with MS detectors. And shorter 50, 75 and 100 mm long
columns can be a great starting choice, with longer columns used only when more resolution is
needed or when 3.5 and 5 µm particle sizes are used.

LC AND LC/MS COLUMNS

797

LC and LC/MS Columns

Silica Type and Bonded Phase
Base Material
The base material for an LC column is most often high purity silica material with totally porous
particles such as that used in most Agilent ZORBAX columns. However, more choices are
available, including polymer material with high pH stability used in PLRP-S columns and
superficially porous silica particles such as those used in Poroshell 120 columns. The high purity
Type B silicas, including the ZORBAX Rx-Sil used in ZORBAX Eclipse Plus, and superficially porous
Poroshell 120, are an excellent first choice for most methods. Type A silicas, such as ZORBAX
SIL, used in Original ZORBAX columns, are still manufactured and used in many methods.

Bonded Phase
A good first choice for bonded phase is C18 or C8, and the recommended starting column
choices are Eclipse Plus C18 or Poroshell 120 EC-C18. These two choices provide excellent peak
shape and can be used over the pH range 2-9, accommodating most typical LC and LC/MS
mobile phases. If the sample solutes of interest are not adequately separated on these columns,
CN and Phenyl columns – including Phenyl, Phenyl-Hexyl and Diphenyl – may offer significant
differences in selectivity from straight-chain alkyl phases to effect the separation.

pH and Mobile Phase
The choice of mobile phase for a reversed-phase system starts with selecting the organic
modifier. Acetonitrile is the most commonly used organic modifier. However, selectivity
differences and sample retention will vary significantly among mobile phases containing
acetonitrile, methanol, and tetrahydrofuran (THF). Sample solubility is likely to differ in such
solvents and dictate use of a specific solvent or solvents. UV detection at certain wavelengths is
not possible with certain modifiers (e.g., methanol at 200 nm).

Tips & Tools
Agilent offers a variety of e-Seminars and on-site training
to help you learn how to be a more effective chromatographer.
For more information, visit www.agilent.com/chem/education

798

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l 01357 522 961

LC and LC/MS Columns

HPLC Column

Stationary
Phase

Type of
Surface

Pore Size

Column
Dimensions

Particle
Size

Inner
Diameter

Length

Chemical Properties

Physical Properties

Chemical Lifetime/Selectivity
Retention Factor

Efficiency
Sensitivity
Speed

Column Choice Relative to Application Objective
Application
Very high sensitivity, LC/MS, peptides and proteins
Very high sensitivity, limited sample, LC/MS, peptides and proteins
High sensitivity, limited sample, LC/MS
Save solvent; special low-volume instrumentation is available
Special detectors, e.g., mass spec
High sensitivity, limited sample
Save solvent; standard HPLC equipment available, LC/MS
Standard separations
Small-scale (mg) preparative separations
Large-scale preparative separations (100 mg-gram)
Large-scale preparative separations (up to 100 mg-gram)

Objective Column
Diameter (mm)
0.1, 0.075
0.3, 0.5
1.0
2.1
2.1
2.1
3.0
4.6
9.4
21.2
30, 50

Consult the Column Hardware section for guard column configurations

LC AND LC/MS COLUMNS

799

Method Development

Recommended Column Choices for Method Development

Small Molecules

Large Molecules

MW < 2000

MW > 2000

Packing Pore Size

80-120Å

300Å

The first choice of a packing pore size is based on the size of molecules to be analyzed. Typical small molecules can diffuse easily in and out of standard
80-120Å pore packings, but peptides and proteins may not. For this reason, use 300Å pore packings (300SB) for isocratic or gradient separations
of peptides and proteins.

Starting Column Bonded Phase

Eclipse Plus C18

StableBond 300SB-C18

C18 is recommended as the starting column bonded phase for most samples since it maximizes retention for moderately polar to non-polar compounds.
Shorter chain phases should be considered if resolution cannot be optimized with a C18 phase or if you are analyzing larger proteins.

Starting Column Choices
Small Molecules

Large Molecules

Standard Analysis

Fast Analysis

Standard Analysis

Fast Analysis

Eclipse Plus C18

Poroshell 120 EC-C18

ZORBAX 300SB-C18

ZORBAX 300SB-C18

4.6 x 150 mm, 3.5 µm

4.6 x 100 mm, 2.7 µm

4.6 x 150 mm, 5 µm

4.6 x 50 mm, 3.5 µm

959963-902

695975-902

883995-902

865973-902

Poroshell 120 EC-C18

Rapid Resolution HD Eclipse
Plus C18, 1200 bar

4.6 x 75 mm, 2.7 µm

Poroshell 300SB-C18
2.1 x 75 mm, 5 µm

2.1 x 50 mm, 1.8 µm
697975-902

660750-902
959757-902

800

LC AND LC/MS

www.agilent.com/chem/lccolumns
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l 01357 522 961

USP Designations

USP Designations
The US Pharmacopeia (USP) is a standard source for many pharmaceutical methods that specifies columns by packing materials rather than
by manufacturer. Listed below are the recommended Agilent Technologies HPLC columns suitable for most LC methods listed with the USP.

USP Method

USP Packing Materials

Column

Particle Size (µm) Page No.

L1

Octadecyl silane chemically bonded to porous silica
or ceramic micro-particles, 1.5 to 10 µm in diameter

2.7
2.7
1.8, 3.5, 5
1.8, 3.5, 5, 7
1.8, 3.5, 5, 7
3.5, 5
1.8, 3.5, 5, 7
3.5, 5, 7
5
3, 5, 10
3, 5, 10
3, 5, 10
3, 5
10
10

822
822
827
831
838
854
850
870
870
862
860
867
867
928
928

L2

Octadecyl silane chemically bonded to porous silica gel
of a controlled surface porosity that has been bonded
to a solid spherical core, 30 to 50 µm in diameter
Porous silica particles, 5 to 10 µm in diameter

Poroshell 120 EC-C18
Poroshell 120 SB-C18
ZORBAX Eclipse Plus C18
ZORBAX Eclipse XDB-C18
ZORBAX SB-C18
ZORBAX Rx-C18
ZORBAX Extend-C18
ZORBAX ODS
ZORBAX ODS classic
Pursuit XRs C18
Pursuit C18
Polaris C18-A
Polaris C18-Ether
SepTech ST60 C18
SepTech ST150 C18
N/A

ZORBAX SIL
ZORBAX Rx-Sil
Pursuit XRs Si
Polaris Si-A
MicroSpher Si
Microsorb 100 Si

5
3.5, 5
3, 5, 10
5, 10
5
5

873
873
862
867

L3

L4
L5
L6

Silica gel of controlled surface porosity bonded to
a solid spherical core, 30 to 50 µm in diameter
Alumina of controlled surface porosity bonded to
a solid spherical core, 30 to 50 µm in diameter
Strong cation-exchange packing: sulfonated
fluorocarbon polymer coated on a solid spherical core,
30 to 50 µm in diameter

N/A
N/A

(Continued)

LC AND LC/MS COLUMNS

801

USP Designations

USP Method

USP Packing Materials

Column

Particle Size (µm) Page No.

L7

Octyl silane chemically bonded to totally porous
microsilica particles, 1.5 to 10 µm in diameter

An essentially monomolecular layer of aminopropylsilane
chemically bonded to totally porous silica gel support,
10 µm in diameter

L9

10 µm irregular, totally porous silica gel having
a chemically bonded, strongly acidic cation
exchange coating
Nitrile groups chemically bonded to porous silica
particles, 3 to 10 µm in diameter

2.7
1.8, 3.5, 5
1.8, 3.5, 5, 7
1.8, 3.5, 5, 7
1.8, 3.5, 5, 7
5
3, 5, 10
3, 5, 10
3, 5
3, 5
5
5
5
5
5 spherical

822
827
831
838
854
870
856
856
865
865

L8

Poroshell 120 EC-C8
ZORBAX Eclipse Plus C8
ZORBAX Eclipse XDB-C8
ZORBAX SB-C8
ZORBAX Rx-C8
ZORBAX C8
Pursuit XRs C8
Pursuit C8
Polaris C8-A
Polaris C8-Ether
Microsorb 100 C8
ZORBAX NH2
Polaris NH2
Microsorb 100 Amino
ZORBAX SCX

ZORBAX CN
ZORBAX SB-CN
ZORBAX Eclipse XDB-CN
Microsorb 100 Cyano
ZORBAX TMS

5
3.5, 5
3.5, 5
5
5

873
838
831

ZORBAX SAX
IonoSpher A

5

879

8

881

Hi-Plex Ca
Hi-Plex Ca (Duo)

8
8

881
881

LiChrospher Diol

5

L10

L13
L14

L15
L16
L17

L18
L19

L20

Trimethylsilane chemically bonded to porous silica
particles, 3 to 10 µm in diameter
Silica gel 10 µm in diameter with a chemically
bonded, strongly basic quaternary ammonium
anion exchange coating
Hexyl silane chemically bonded to totally porous silica
particles, 3 to 10 µm in diameter
Dimethyl silane chemically bonded to totally porous silica
particles, 3 to 10 µm in diameter
Strong cation exchange resin consisting of sulfonated
cross-linked styrene-divinylbenzene copolymer in the
hydrogen form, 7 to 11 µm in diameter
Amino and cyano groups chemically bonded to porous
silica particles, 5 to 10 µm in diameter
Strong cation exchange resin consisting of sulfonated
cross-linked styrene-divinylbenzene copolymer in the
calcium form, 9 µm in diameter
Dihydroxypropane groups chemically bonded to porous
silica particles, 3 to 10 µm in diameter

873
865
879

MetaSil C6
N/A
Hi-Plex H

N/A

(Continued)

802

LC AND LC/MS

www.agilent.com/chem/lccolumns
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l 01357 522 961

USP Designations

USP Method

USP Packing Materials

Column

Particle Size (µm) Page No.

L21

A rigid, spherical styrene-divinylbenzene copolymer,
5 to 10 µm in diameter

L22

A cation exchange resin made of porous polystyrene gel
with sulfonic acid groups, about 10 µm in size
An ion exchange resin made of porous polymethacrylate
or polyacrylate gel with quaternary ammonium groups,
about 10 µm in size
A semi-rigid hydrophilic gel consisting of vinyl polymers
with numerous hydroxyl groups on the matrix surface,
32 to 63 µm in diameter
Packing having the capacity to separate compounds
with a MW range from 1,000 to 5,000 da
(as determined by the polyethylene oxide), applied
to neutral, ionic and cationic water-soluble polymers
Butyl silane chemically bonded to totally porous silica
particles, 5 to 10 µm in diameter
Porous silica particles, 30 to 50 µm in diameter
A multifunctional support, which consists of a high
purity, 100Å, spherical silica substrate that has been
bonded with anionic (amine) functionality in addition
to conventional reversed-phase C8 functionality
Gamma alumina, reversed phase, low carbon
percentage by weight, alumina-based polybutadiene
spherical particles, 5 µm diameter with a pore diameter
of 80Å
Ethyl silane chemically bonded to a totally porous silica
particle, 3 to 10 µm in diameter
A strong anion-exchange resin-quaternary amine
bonded on latex particles attached to a core of 8.5 µm
macroporous particles having a pore size of 2000Å
and consisting of ethylvinylbenzene cross-linked with
55% divinylbenzene
A chiral ligand-exchange packing L-proline copper
complex covalently bonded to irregularly shaped silica
particles, 5 to 10 µm in diameter
Packing having the capacity to separate proteins by
molecular size over a range of 4,000 to 400,000 da.
It is spherical, silica-based, and processed to provide
pH stability

PLgel
PLRP-S 100Å
PLRP-S 300Å
PLRP-S 1000Å
Hi-Plex H

3, 5, 10, 20
3, 5, 8
3, 5, 8
5, 8
8

947
1027
1027
1027
881

PL aquagel-OH

5, 8

974

MicroSorb C4

5

L23

L24

L25

L26
L27
L28

L29

L30
L31

L32

L33

N/A

N/A

Bondesil Silica
N/A

204

N/A

N/A
N/A

N/A

ZORBAX GF-250
Bio SEC-3
Bio SEC-5

4
3
5

1001
997
999
(Continued)

LC AND LC/MS COLUMNS

803

USP Designations

USP Method

USP Packing Materials

Column

Particle Size (µm) Page No.

L34

Strong cation exchange resin consisting of sulfonated
cross-linked styrene-divinylbenzene copolymer in the
lead form, 9 µm in diameter
A zirconium-stabilized spherical silica packing
with a hydrophilic (diol-type) molecular monolayer
bonded phase
L-Phenylgylcine-3,5-dinitrobenzoyl on 5 µm amino
propyl silica
Polymethacrylate gel packing having the capacity
to separate proteins by molecular size over a range
of 2,000 to 4,000 da MW
Methacrylate-based size exclusion packing
for water solubles
Hydrophilic polyhydroxymethacrylate gel of totally
porous spherical resin
Cellulose tris-3,5-dimethylphenylcarbamate coated
porous silica particles, 5 to 20 µm in diameter
Immobilized alpha-acid glyco-protein on spherical silica
particles, 5 µm in diameter
Octylsilane and octadecylsilane groups chemically
bonded to porous silica particles
Pentafluorophenyl groups chemically bonded to silica
particles 5 to 10 µm in diameter
A multifunctional support, which consists of a high
purity, 60Å spherical silica substrate, that has been
bonded with a cationic exchanger, sulfonic acid
functionality in addition to a conventional reversed
phase C8 functionality
Beta cyclodextrin bonded to porous silica particles,
5 to 10 µm in diameter
Polystyrene/divinylbenzene substrate agglomerated
with quaternary amine functionalized latex beads,
10 µm in diameter
High capacity anion exchange microporous substrate,
fully functionalized with a trimethyl-amine group,
8 µm in diameter
Sulfonated, cross-linked polystyrene with an outer layer
of submicron, porous, anion-exchange microbeads,
15 µm in diameter
Amylose tris-3,5-dimethylphenyl-carabamate-coated,
porous, spherical, silica particles, 5 to 10 µm in diameter

Hi-Plex Pb

8

881

ZORBAX GF-250
ZORBAX GF-450

4
6

1001
1001

3, 5

856

5

915

L35

L36
L37

L38
L39
L40
L41
L42
L43
L44

L45
L46

L47

L48

L49

N/A
N/A

N/A
N/A
N/A
N/A
N/A
Pursuit PFP
N/A

ChiraDex Chiral
N/A

N/A

N/A

N/A

(Continued)

804

LC AND LC/MS

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USP Designations

USP Method

USP Packing Materials

Column

Particle Size (µm) Page No.

L50

A strong cation exchange resin made of porous silica
with sulfopropyl groups, 5 to 10 µm in diameter
A reversed-phase packing made by coating a thin layer
of polybutadiene on to spherical porous zirconia
particles, 3 to 10 µm in diameter
Multifunction resin with reversed-phase retention
and strong anion-exchange functionalities. The resin
consists of ethylvinyl-benzene, 55% cross-linked
with divinylbenzene copolymer, 3 to 15 µm in diameter,
and a surface area of not less than 350m2/g, substrate
is coated with quaternary ammonium functionalized
latex particles consisting of styrene cross-linked
with divinylbenzene.
An anion-exchange resin consisting of rigid, spherical
styrene-divinylbenzene copolymer with
trimethylammonium groups at a loading of about 2 meq
per g, 3 to 29 µm in diameter
Strong cation-exchange resin consisting of sulfonated
cross-linked styrene-divinylbenzene copolymer in
the sodium form, about 7 to 11 µm diameter
Weak cation-exchange resin consisting of
ethylvinylbenzene, 55% cross-linked with divinylbenzene
copolymer, 3 to 15 µm diameter. Substrate is surface
grafted with carboxylic acid and/or phosphoric acid
functionalized monomers. Capacity not less than
500 µeq/column
Propyl silane chemically bonded to totally porous silica
particles, 3 to 10 µm in diameter
A chiral-recognition protein, ovomucoid, chemically
bonded to silica particles, about 5 µm in diameter,
with a pore size of 120 angstroms
Strong cation-exchange resin consisting of sulfonated
cross-linked styrene-divinylbenzene copolymer in the
sodium form, about 6 to 30 µm in diameter
Packing having the capacity to separate proteins by
molecular weight over the range of 5 to 7000 kDa. It is
spherical (5-10 µm), silica-based, and processed to
provide hydrophilic characteristics and pH stability
Spherical, porous silica gel, 10 µm or less in diameter,
the surface of which has been covalently modified
with alkyl amide groups and endcapped

ZORBAX 300SCX

5

879

3, 5, 10

1006

SB-C3

3, 5

838

Ultron ES-OVM

5

913

Hi-Plex Na
Hi-Plex Na (Octo)

10
8

881
881

1.8, 3.5, 5
3, 5

846
865

L51

L52

L53

L54

L55

L56
L57

L58

L59

L60

N/A

N/A

Bio SAX

N/A

N/A

N/A

Bonus-RP
Polaris Amide-C18

LC AND LC/MS COLUMNS

805

Cartridge Column Systems

CARTRIDGE COLUMN SYSTEMS
Cartridge Selection Guide
Icon*

Type of Cartridge
Agilent HPLC Cartridge

ZORBAX Guard Cartridge:
Standalone system

ZORBAX Rapid Resolution
and Rapid Resolution HT
Cartridge Columns:
3.5 µm and 1.8 µm packings,
standalone system
ZORBAX Semi-Preparative Guard
HPLC Hardware Kit:
Standalone system
ZORBAX and Agilent Prep
Preparative Cartridge Column
and Guard HPLC System:
Standalone and integral
hardware options
ChromSep Column Hardware:
Complete systems
and replacement cartridges

Features
Can reverse collets in the end fitting to add
guard cartridges

Cartridges have a unique filter and sieve
at each end
High efficiency, standalone,
Seals up to 400 bar
low dead volume cartridge
Polymeric cartridge designed for leak-tight seals No gaskets required
against metal surfaces
More solvent-resistant than PEEK
Reusable fittings
Adapt for connections to 1/16 in. LC fittings
For high throughput LC/MS, LC/MS/MS
and combinatorial separations
Packed with Eclipse XDB for pH use from 2-9 For all analyte types
Packed with StableBond for low pH use
Low bleed
Sold individually or as three-packs
Easy, low-dead-volume assembly
Seals up to 2000 psi (135 bar, 13.5 MPa)
Tubing (polyphenylene sulfone) designed
No gaskets required
for leak-tight seals against metal surfaces
Reusable fittings
Adapt for connections to 1/16 in. LC fittings
Easy, low-dead-volume assembly
Extends column lifetime
Reusable fittings
Permits rapid column changes
Hardware options for integral
Can use with 21.2 and 30 mm ID columns
and external guards
Easy, no-dead-volume assembly

*Look for these icons on subsequent pages to help you select the proper guard cartridges and columns.

806

LC AND LC/MS

Benefits
Inexpensive
Extends column lifetime
Permits rapid column changes
Can use 2, 3, 4 and 4.6 mm cartridges
Helps prevent blockage

www.agilent.com/chem/lccolumns
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l 01357 522 961

Economical format
No tools required
Modular flexibility

Cartridge Column Systems

Cartridge/Guard Cartridge Systems Compatibility Guide*
Icon

Column Type
Cartridge column cartridge holder
5021-1845

Guard Cartridge Holder
Guard cartridge (internal system) cartridge holder
5021-1845

ID (mm)
2.0
3.0
4.0
4.6

Phases
Asahipak
LiChrospher
Nucleosil
Purospher
Superspher
ZORBAX

Standard fitting

Column guard cartridge (standalone)
cartridge holder
820999-901

2.1
3.0
4.6

ZORBAX

Rapid Resolution cartridge holder
820555-901

No guard cartridge holder

4.6

ZORBAX

Semi-preparative column

Semi-prep guard cartridge (standalone)
cartridge holder
840140-901

9.4

ZORBAX

PrepHT

Guard cartridge
820444-901

21.2

ZORBAX
Agilent Prep

*Standalone guard cartridges fit all cartridge and standard fitting columns available from Agilent. All columns without icons are standard fitting columns.

LC AND LC/MS COLUMNS

807

Cartridge Column Systems

Cartridge Column Systems
Look for this icon identifying Agilent cartridge
columns in column ordering tables

Agilent offers a variety of popular HPLC packing materials in economical, easy-to-use
cartridge configurations.

Agilent Cartridge System
Agilent’s flexible cartridge system has been thoroughly tested to ensure that the design and
hardware meet Agilent’s quality standards. Finger-tight connections allow rapid column changes
without removing capillaries from end fittings. The same convenient, easy-to-use cartridge holder
accommodates 2, 3, 4 and 4.6 mm diameter cartridges of varying lengths. The cartridge columns
have a unique filter and sieve at each end that help prevent blockage.
By reversing the collets in the end fitting, an inexpensive guard cartridge can be added to further
extend column lifetime.
Guard cartridge installed

Hardware
Description
Cartridge holder for 2, 3, 4 and 4.6 mm ID cartridges
Replacement filters for 4 and 4.6 mm ID cartridges
Replacement filters for 2 and 3 mm ID cartridges
Mounting tool for replacement filters
Replacement collets

No guard cartridge installed

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

808

LC AND LC/MS

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l 01357 522 961

Unit
2/pk
10/pk
10/pk
2/pk

Part No.
5021-1845
5063-6574
5063-6519
5021-1846
5021-1849

Cartridge Column Systems

ZORBAX High Performance Guard Cartridge
This icon identifies standalone guard cartridges
for ZORBAX analytical columns

The ZORBAX High Performance Guard Cartridge series has been developed to provide
convenient, cost-effective protection for high performance analytical columns. The cartridge
components assemble quickly and easily to provide a high efficiency, low dead volume column
that seals, with hand tightening, up to 5000 psi (340 bar) or 3000 psi with a PEEK fitting.
The reusable guard column end fitting with integrated 1/16 in. OD tubing adapts the cartridge
guard column for direct connection to standard 1/16 in. LC fittings and provides a standalone
guard column system for 2.1 to 4.6 mm ID columns. There are two different end fitting options
to allow the use of other connecting tubing.
The polymeric guard cartridges used in this holder are specifically designed to make
leak-tight seals against metal surfaces without requiring gaskets. This polymeric material
(polyphenylenesulfone) is also more solvent resistant than PEEK.
Guard cartridges are available for almost every ZORBAX bonded phase and can be found in the
ordering information for each type of column.

Hardware
Description
Guard fittings kit
Includes low-volume guard holder, inlet end fitting (2), outlet end fitting with
integrated column connector, and PEEK fingertight fitting
Inlet end fitting, also used as alternate outlet end fitting
Exit end fitting with integrated column connector
1/16 in. finger-tight PEEK fitting, 2/pk
Perfluoro-Elastomer Seals, 2/pk

Part No.
820999-901

820340-001
820345-001
0100-1516
820370-901

LC AND LC/MS COLUMNS

809

Cartridge Column Systems

Rapid Resolution and Rapid Resolution HT
Cartridge Column System (400 bar)
Rapid Resolution and Rapid Resolution HT
Cartridge Columns are marked with this icon

For fast, clean high throughput LC/MS, LC/MS/MS and combinatorial separations,
we recommend ZORBAX Rapid Resolution (3.5 µm) and Rapid Resolution HT (1.8 µm)
Cartridge Columns. These cartridges are packed with ZORBAX Eclipse and StableBond
bonded phases that provide excellent separations.
Cartridge dimensions are 4.6 x 15 mm, 4.6 x 30 mm or 4.6 x 50 mm and 2.1 x 15 mm,
2.1 x 30 mm or 2.1 x 50 mm. All 15 and 30 mm cartridges are available in both Eclipse
and StableBond phases in both the 3.5 µm and the very high efficiency 1.8 µm particles.
The 1.8 µm particles are available as 50 mm cartridges and as 50 mm columns with fixed
endfittings. Choose the Eclipse XDB bonded phases for most methods and when using
LC/MS mobile phase additives such as formic acid or acetic acid. The StableBond phases
are ideal for different selectivity and for long lifetime with TFA-containing mobile phases.
Additional bonded phases can be packed upon request.
These economical and easy-to-use cartridge columns are offered individually and in
convenient three-packs.
One cartridge holder kit includes all components for use with Rapid Resolution
or Rapid Resolution HT columns.

Hardware
Description
Hardware Kit for RR and RRHT Cartridges
Includes cartridge holder 15 mm, cartridge holder 30 mm, cartridge holder 50 mm
(1 ea), and end fitting assemblies (2)
Cartridge holder, 15 mm
Cartridge holder, 30 mm
Cartridge holder, 50 mm
Perfluoro-Elastomer Seals, 2/pk
End fitting assembly, two required for one system

810

LC AND LC/MS

www.agilent.com/chem/lccolumns
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l 01357 522 961

Part No.
820555-901

820315-015
820330-030
820320-050
820370-901
820311-001

Cartridge Column Systems

ZORBAX Semi-Preparative Guard Column Hardware Kit
This icon identifies preparative guard columns

The ZORBAX Semi-Preparative Guard Column has been developed to provide convenient,
cost-effective protection for high-performance lab-scale semi-preparative columns. The cartridge
components assemble quickly and easily to provide a high-efficiency, low-dead-volume column
that seals at pressures up to 2000 psi (135 bar, 13.5 MPa).
The guard column housing made from polyphenylene sulfone is specifically engineered to make
leak-tight seals against metal surfaces, without requiring gaskets. The reusable guard-column
end fittings adapt the cartridge guard column for connection to standard 1/16 in. LC fittings
and provide a standalone guard column system. The ZORBAX materials used in preparative
cartridges are matched with chemistry chosen for compatibility with a wide range of applications.

Hardware
Description
Preparative guard column hardware kit*
Includes inlet fitting, outlet end fitting, column connector
Preparative guard system

Part No.
840140-901

*The semi-preparative guard column hardware is available only as a kit.

LC AND LC/MS COLUMNS

811

Cartridge Column Systems

ZORBAX PrepHT and Agilent Prep Preparative Cartridge
and Guard Column Hardware
This icon identifies prep preparative cartridge
and guard columns

Guard Cartridge, 820444-901

The ZORBAX PrepHT and Agilent Prep Preparative Cartridge and Guard Column hardware
kits have been developed to provide a convenient preparative 21.2 mm ID cartridge design.
The 21.2 mm ID preparative cartridge columns (actual ID 17 mm to fit into holder) are reusable
and allow rapid change of column lengths from 50 to 250 mm for optimizing sample loadability.
This easy-to-use cartridge hardware design is used for both ZORBAX PrepHT and Agilent Prep
materials and can be finger-tightened up to 5000 psi (350 bar).
The cartridge hardware can be used standalone or with an integral guard column. The integral
guard column holder is a stainless steel body and is used with a PTFE sealing gasket to ensure
a tight, leak-free and extremely low-dead-volume seal against the 21.2 mm ID cartridge body.
The external guard system seals finger-tight up to 2000 psi (135 bar). The reusable guard
holder is ready-to-use with standard 1/16 in. LC fittings. Both ZORBAX and Agilent Prep
guard cartridges are available to use with this holder and are selected to match
the preparative column used in the application.
The 21.2 mm ID guard columns can be used with 30 mm ID Agilent Prep columns.
For this application, select the external preparative guard column hardware kit.

Prep external guard hardware kit, assembled,
420420-901

PrepHT Columns are easy to use
Column Cartridge
Seal Holder and Seal Pusher
Guard Column End Fitting

Standard End Fitting

Guard Column
Polymeric Seal

Polymeric Seals

PrepHT cartridge columns have a unique design that makes them easy to install and seal
finger-tight up to 5000 psi. The cartridge design allows for an integral guard column to be
used, which prolongs the life of the purification column. This cartridge configuration is
economical to use since the column cartridge and/or the guard cartridges are replaced
independently. The end fittings are used many times.

812

LC AND LC/MS

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l 01357 522 961

Cartridge Column Systems

Hardware
Description
PrepHT cartridge column hardware
Includes cartridge column end fittings (2), polymeric seals (2)
PrepHT guard column hardware kit
Includes guard column end fitting, polymeric seal, and seal insertion tool
(seal holder and seal pusher)
Agilent Prep external guard hardware kit, Includes guard holder, guard column
end fitting, polymeric seal (2), seal insertion tool, and connector tubing
Replacement polymeric seals, 2/pk

Part No.
820400-901
820444-901

420420-901
820385-901

ChromSep HPLC Column Hardware
This icon identifies ChromSep column hardware

The ChromSep system combines simplicity with extraordinary flexibility and considerable savings
on column and operating costs. The ChromSep 316 stainless steel column-housing hardware
is a durable one-time investment. Once you have purchased the complete basic system of
a holder, analytical cartridge and guard column, you will only need to replace the cartridges
or replacement guard columns, both of which are available in economical packages: 3-pack
analytical column replacements and 5-pack guard column replacements for added value.
Unlike other modular column systems, ChromSep is extremely flexible. Column housings are
available in lengths of 10, 30, 50, 100, 150 and 250 mm, and cartridges are available in various
IDs ranging from 2 to 4.6 mm. You can use any combination of cartridge columns to match the
column length with the separation you need and minimize your analysis time.

Tips & Tools
Guard columns and filters help protect your column and
instrument from particulates that can cause blockages, which
increase system pressure and negatively impact performance.
Learn more about this common problem at
www.agilent.com/chem/lctroubleshooting

LC AND LC/MS COLUMNS

813

HPLC Column Protection

HPLC Column Protection
Column Protection
Guard columns and in-line filters are inexpensive and easy-to-use tools for column protection.
They can improve the accuracy of your results and improve analytical column lifetime while
enhancing reliability. Column protection is available for all sizes of columns with any particle
size packed into the column.

Guard Columns
Guard columns provide protection against contamination with minimal impact on column
efficiency. Prepacked ZORBAX cartridge columns are available for most types of ZORBAX
material. Guard cartridges are available in different internal diameters to provide high efficiency
protection to all types of columns. Guard columns are also available for many non-Zorbax
columns. See the respective column listings for available guard columns.

Low Volume In-line Filters
Low volume in-line filters are recommended for every column and provide column protection
from particulate materials. An in-line filter will increase analytical column lifetime by preventing
particulates (from unfiltered samples and/or eluents) from plugging the analytical column frit.
Using guard columns can compromise the efficiency of very low volume columns and/or columns
with very small particle sizes. For these columns, low volume in-line filters are strongly
recommended. A small, 0.5 µm frit should be used to maximize column efficiency.

Replacement Column Inlet Frits
If HPLC columns are used without a guard column on in-line precolumn filters, the analytical
column may become plugged. Due to the high efficiency packing processes used today,
replacing the column inlet frit is discouraged. Column efficiency may be compromised if
the frit is replaced. PEEK-encapsulated replacement frits are available for ZORBAX columns
packing in 2.1, 3.0, 4.6, and 9.4 mm standard column hardware.

Replacement Inlet Frits (PEEK Encapsulated)
for Standard Hardware Columns
Description
Narrow Bore
Solvent Saver
Analytical
Semi-Preparative

814

LC AND LC/MS

www.agilent.com/chem/lccolumns
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Diameter (mm)
2.1
3.0
4.6
9.4

l 01357 522 961

Unit
10/pk
1/ea
10/pk
1/ea

Part No.
280959-904
280959-006
280959-905
280959-001

Agilent ZORBAX Silica

Agilent ZORBAX Silica
ZORBAX Silica Manufacturing Process –
the Making of a Rugged, High-Purity Silica
All Agilent ZORBAX columns are built from porous silica microspheres (PSM) based on silica sols.
The silica particle is made of tiny, solid sol microparticles agglutinated in a patented
polymerization process, then fused together at very high temperatures to form the final particle
(Figure 1). These strong, durable silica particles are called ZORBAX Rx-SIL or ZORBAX SIL and
are the base silicas for ZORBAX columns.
The ZORBAX Rx-SIL process produces ultra-pure (99.995%) particles, with very low metal
content. The final silica particle is fully hydroxylated and of low acidity. The Rx-SIL process also
allows careful and reproducible control of pore size and particle size. These key features – purity
(low acidity), strength, and careful control of pore and particle size – are critical to excellent
chromatographic results and are the building blocks of superior ZORBAX bonded phases.
The table compares the processes used to make the ZORBAX Rx-SIL particles to a second
process – the Xerogel process – commonly used to make silica particles for HPLC columns.
To produce silica with the key features that maximizes chromatographic performance – purity,
strength, controlled pore and particle size, plus higher pH resistance – the Agilent ZORBAX
process is an excellent choice.

80Å or 300Å pore

SilicaSol
+
Urea (pH~2)
+
CH 2O

O2

1.8
3.5 µm
5, 7

Eclipse Plus
Eclipse XDB
StableBond SB, 300SB
Bonus-RP
Extend-C18, 300Extend-C18

Figure 1. Formation of ZORBAX porous silica particles

LC AND LC/MS COLUMNS

815

Agilent ZORBAX Silica

Characteristics of ZORBAX Rx-SIL and a Contrasting Type of Silica

ZORBAX Rx-SIL uniform sub particles

Structure
Purity
Strength
Pore Size, Particle Size Distribution
Pore Size/Surface Area
Porosity (%)
High pH Resistance

ZORBAX Rx-SIL (Sol-type)
High (99.995%)
High
Narrow
80Å/180 m2/g
60
Good

Xerogel (SIL-type)
Low to High
Moderate
Broad
100Å/300 m2/g
70
Poor

The Benefit of Silica Purity – Reduced Peak Tailing
Xerogel "sponge-like" polymeric network

Peak tailing of basic compounds can be a major chromatographic problem. Peak tailing reduces
chromatographic efficiency and the accuracy and precision of results. The major cause of peak
tailing is interactions between analytes and the silica surface (Figure 2). Typically the presence of
acidic silanol sites on the silica surface cause this type of peak tailing. Trace metals in silica
increase silanol acidity and peak asymmetry. These silanol interactions are reduced or eliminated
by choosing a less acidic, ultra pure (99.995%) silica, such as ZORBAX Rx-SIL. The improvement
in chromatography is dramatic. Figure 3 shows the reduction in peak tailing for a basic analyte
using ZORBAX Rx-SIL versus a more acidic silica.

Silica
Particle

Original ZORBAX SIL (1973)

SiO - Na + + R3NH +

Silica
Particle

SiO - NH +R 3 + Na +

1. Ionized silanols (SiO- ) will ion-exchange with protonated
bases (R 3NH +) which can cause tailing and method variability

CH 2-OH

.

NH 2

Silica
Particle -SiOH + RCOO -

CH 2CH 3

Silica
+
Particle -SiO - ...H ... - OOCR

2. Unprotonated acids can compete for H + with protonated silanols.

Figure 2. Potential secondary interactions with silica silanols and ionizable compounds

Highly Purified ZORBAX Rx-SIL

NH 2
CH 2CH 3

CH 2-OH

Mobile Phase:

5% Propanol in Heptane

Flow Rate:

2.0 mL/min

Figure 3. Chromatographic improvement using highly
purified ZORBAX Rx-SIL

816

LC AND LC/MS

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l 01357 522 961

Agilent ZORBAX Silica

The Benefits of Strong Particles –
Greater Efficiency and Durability
During the silica manufacturing process, the agglutinated sol particles are sintered for increased
strength. This improved mechanical stability allows ZORBAX columns to be packed at high
pressures when needed – up to 14,000-15,000 psi. This results in a packed column with an
exceptionally stable column bed that will not compress under normal or even high operating
pressures – up to 18,000 psi (1200 bar). This packed bed stability increases column lifetime
using ZORBAX 1.8, 3.5, 5 or 7 um particles. When ZORBAX Rapid Resolution HD or HT 1.8 µm
and Rapid Resolution 3.5 µm silica particles are used as the underlying support, high speed, high
efficiency chromatography is possible without compromising column lifetime.

The Benefits of Careful Pore Size and Particle Size Control –
High Efficiency and Better Reproducibility
with More Column Choices
Accurate and closely monitored particle and pore size control for ZORBAX Rx-SIL produces
reproducible retention behavior from column-to-column and lot-to-lot. The narrow, consistent
particle size distribution of ZORBAX Rx-SIL particles maximizes efficiency and column bed
stability. Column pressure is never unusually high due to "fines" – smaller particles at the low
end of the particle size distribution. Accurate and precise control of particle size allows specific
1.8, 3.5, 5 and 7 µm particles to be produced. The small 3.5 µm and 1.8 µm particle sizes are
the basis for the Rapid Resolution and Rapid Resolution HD and HT, high-speed analysis columns
designed to maximize resolution in shorter column lengths – ideal for LC/MS or any application
demanding shorter analysis times. The 5 µm particles are an industry standard and provide
high resolution in a wide variety of column dimensions. This particle size also provides high
efficiency in a short preparative configuration – the PrepHT column – because careful particle
size control means consistent pressure expectations within normal operating limits. The 7 µm
particle size provides the ideal balance between efficiency and operating pressure for longer
preparative columns.

ZORBAX Rx-SIL – The Foundation for Many Bonded Phases
With such strong performance characteristics, ZORBAX Rx-SIL particles have been developed
into many effective bonded phases for solving key analytical problems. These include columns
that can be used at extremes of pH, unmatched by any other silica-based columns. Because
silica-based columns have different limitations at low and high pH, specific bonded-phase
chemistries are required to provide longer column life over different pH ranges. As a result,
Agilent ZORBAX RP-HPLC bonded phases are designed to give extended column lifetime
and reproducibility in the pH ranges that provide optimum and long-lasting resolution,
all starting with high performance ZORBAX Rx-SIL.

LC AND LC/MS COLUMNS

817

Columns for Analytical HPLC

AGILENT COLUMNS
FOR ANALYTICAL HPLC
Achieve excellent peak shape and resolution –
and eliminate "false starts"
Good news for analysts who do not have time to "make columns work" for a particular
application: Agilent columns let you choose the right column based on your sample and mobile
phase – eliminating any guesswork.
Additionally, Agilent’s ZORBAX silica is manufactured by Agilent – not purchased from outside
suppliers. And that means we control every step of the manufacturing process, ensuring lot-to-lot
consistency, superior performance, and long-term, reliable results.
In this section, you will find a diverse range of columns designed for optimum resolution over a
wide pH range, including:

• Poroshell 120 HPLC Columns
• ZORBAX Reversed-Phase HPLC Columns
• Pursuit HPLC Columns
• Polaris HPLC Columns
• ZORBAX Normal-Phase HPLC Columns
• ZORBAX and Hi-Plex Ion Exchange HPLC Columns
• ZORBAX Method Development and Validation Kits

Tips & Tools
All ZORBAX and Poroshell conventional columns (non-cartridge) come with
a QC chromatogram. Run a standard sample of QC components or key
analytes from your lab on each new column before use, and keep this
chromatogram in your log book. Periodically re-run this test to see how your
column has aged. Learn how this can help with troubleshooting issues
by watching the videos at www.agilent.com/chem/lctroubleshooting

818

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

Agilent Reversed-Phase Columns
The following table summarizes the unique bonding chemistry of ZORBAX RP-HPLC columns. Each is designed for long column lifetime
and resolution that lasts.

Agilent RP-HPLC Column Chemistry

Bonus-RP
Extend-C18

B
B

Totally porous
Totally porous

Triple
Double

Rx-C18
Pursuit
Pursuit XRs
Polaris A
Polaris Ether
Polaris Amide

B
B
B
B
B
B

Totally porous
Totally porous
Totally porous
Totally porous
Totally porous
Totally porous

None
Single
Single
Single
Single
Single

Side Group Structure
on Silane
Dimethyl
Diisobutyl
Dimethyl
Dimethyl
Diisopropyl (C8, C3, CN,
phenyl), diisobutyl (C18)
Diisopropyl
Unique bidentate
structure
Dimethyl
Dimethyl
Dimethyl
Dimethyl
Dimethyl
Dimethyl

Original ZORBAX Columns**
ZORBAX
ZORBAX ODS Classic

A
A

Totally porous
Totally porous

Single
None

Dimethyl
Dimethyl

Modern Columns*
Poroshell 120 EC
Poroshell 120 SB
Eclipse Plus
Eclipse XDB
StableBond

Silica Type
B
B
B
B
B

Particle Type
Superficially porous
Superficially porous
Totally porous
Totally porous
Totally porous

Endcapping
Double
None
Double
Double
None

Polar Group
None
None
None
None
None

Page No.
822
822
827
831
838

Amide
None

846
850

None
None
None
Yes
Ether
Amide

854
856
862
865
865
925

None
None

870
870

*Type B silica: low acidity, low metal content; these bonded phases use ZORBAX Rx-SIL
**Type A silica: more acidic, higher metal content

LC AND LC/MS COLUMNS

819

Columns for Analytical HPLC

Quick Guide to Agilent Reversed-Phase Bonded Phases
Modern RP-HPLC
Columns
Poroshell 120

Recommended Uses and Applications
� Superficially porous particles for high efficiency at low pressure
� Sub-2 µm efficiency with a 2.7 µm particle
� Endcapped and non-endcapped C18 and C8 phases for selectivity optimization
� Compatible with 400 bar and 600 bar LC's

Page No.
822

Eclipse Plus

� Excellent first choice for method development
� Long life from pH 2-9 for reliable separations of basic, acidic and neutral compounds
� Superior peak shape with basic compounds
� High resolution and efficiency with 1.8, 3.5 and 5 µm columns
� Rigorous QA/QC testing for greater long-term reproducibility

827

Eclipse XDB

� Four selectivity choices for flexible method development
� High performance over a wide pH range (2-9)
� Good peak shape for acids, bases and neutrals
� Long lifetime with extra dense bonding and double endcapping
� Fast, ultra-fast, and high resolution separations using 1.8 and 3.5 µm columns
� Choices from capillary to prep

831

StableBond (SB)

� Basic, acidic, neutral compounds
� Exceptional stability at low pH (1-2)
� Use of high temperature (up to 90°C for C18, 80°C for C8, C3, Phenyl, CN, and Aq) and low pH as
an added selectivity tool
� Widest selection of bonded phases for different selectivity (C18, C8, C3, CN, Phenyl, Aq)
� Uses mobile phases for LC/MS with formic acid, acetic acid, or TFA
� Uses mobile phases with TFA for peptide and protein separation
� Rapid separations using 1.8 and 3.5 µm columns

838

Bonus-RP

� Separating basic compounds in higher aqueous mobile phases
� General separation of basic, neutral, acidic compounds at mid-range pH or low pH; especially stable
at low pH
� Separating peptides for different selectivity
� Rapid separations using 3.5 µm columns

846

(Continued)

820

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

Quick Guide to Agilent Reversed-Phase Bonded Phases
Modern RP-HPLC
Columns
Extend-C18

Recommended Uses and Applications
� Separating basic compounds above their pKa in free base form; separation of basic, acidic, neutral
compounds at high pH; up to pH 11.5
� Uses ammonium hydroxide as mobile phase additive with LC/MS with small molecules or peptides
� Separating at high, mid-range and low pH for selectivity changes
� Rapid separations using 3.5 µm columns

Page No.
850

ZORBAX Rx

� General separation of basic, acidic and neutral compounds at low pH with different selectivity
than SB columns
� Rx-C8 is the same as SB-C8

854

Pursuit

� Good separations of a wide range of analytes
� Diphenyl and Pentafluorophenyl bonded phases for unique selectivity
� 200Å pore size for separations of larger molecules

856

Pursuit XRs

� High carbon load for excellent retention and resolution
� Basic, acidic, and neutral compounds
� Unique diphenyl bonded phase for separations based on pi-pi selectivity

862

Polaris A

� Good for polar acids, polar bases and non-polar compounds
� High aqueous compatibility

865

Polaris Ether

� Additional selectivity for H-bond donors
� High aqueous compatibility
� No "phase collapse"

865

Recommended Uses and Applications

Page No.
870

Original ZORBAX
Columns
ZORBAX

� General separation of basic, acidic, neutral compounds at low pH with different selectivity than SB columns;
higher number of active silanols than SB
� "Mixed mode" separation at more neutral pH values

ZORBAX ODS Classic � General separation of basic, acidic, neutral compounds at mid-range to low pH with different selectivity
than SB or XDB columns
(non-endcapped)

870

LC AND LC/MS COLUMNS

821

Columns for Analytical HPLC

Poroshell 120
• Up to 90% of the efficiency of sub-2 µm
• 2X the efficiency of 3.5 µm
• Up to 50% less pressure than sub-2 µm columns
• Ideal for use up to 600 bar for HPLC and UHPLC
• Three bonded phases with excellent selectivity and peak shape
Agilent Poroshell 120 columns are a 2.7 µm particle with a 1.7 µm solid core and 0.5 µm porous
outer layer. This small particle size provides high efficiency, similar to sub-2 µm columns, but with
40-50% less pressure. These high efficiency, high resolution columns can be used on any type
of LC. The porous outer layer and solid core limit diffusion distance and improve separation
speed while the narrow particle size distribution improves efficiency and resolution. The solid
core limits diffusion distance and improves separation speed. The columns can support high
pressure and multiple columns can be used for the highest resolution and efficiency possible.
The same principles are used in Poroshell 300 columns, ideal for fast, high resolution
separations of biomolecules.

Column Specifications
Bonded Phase
SB-C18
EC-C18
EC-C8

Pore Size
120Å
120Å
120Å

Temp. Limits pH Range
90°C
1.0-8.0
60°C
2.0-8.0
60°C
2.0-8.0

Specifications represent typical values only.

Tips & Tools
Method transfer from a conventional 3.5 or 5 µm column
is easy, and often requires only minor adjustments to your
method and no revalidation. Learn more at
www.agilent.com/chem/poroshell120video

822

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Endcapped
No
Double
Double

Carbon
Load
8%
10%
5%

Columns for Analytical HPLC

Poroshell 120
Description
Analytical
Analytical
Analytical
Analytical
Analytical
Solvent Saver
Solvent Saver
Solvent Saver
Solvent Saver
Solvent Saver
Narrow Bore
Narrow Bore
Narrow Bore
Narrow Bore
Narrow Bore

Size (mm)
4.6 x 150
4.6 x 100
4.6 x 75
4.6 x 50
4.6 x 30
3.0 x 150
3.0 x 100
3.0 x 75
3.0 x 50
3.0 x 30
2.1 x 150
2.1 x 100
2.1 x 75
2.1 x 50
2.1 x 30

Particle
Size
(µm)
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7

SB-C18
USP L1
683975-902
685975-902
687975-902
689975-902
681975-902
683975-302
685975-302
687975-302
689975-302
681975-302
683775-902
685775-902
687775-902
689775-902
681775-902

EC-C18
USP L1
693975-902
695975-902
697975-902
699975-902
691975-902
693975-302
695975-302
697975-302
699975-302
691975-302
693775-902
695775-902
697775-902
699775-902
691775-902

EC-C8
USP L7
693975-906
695975-906
697975-906
699975-906
691975-906
693975-306
695975-306
697975-306
699975-306
691975-306
693775-906
695775-906
697775-906
699775-906
691775-906

Tips & Tools
Agilent offers a variety of e-Seminars and on-site training
to help you learn how to be a more effective chromatographer.
For more information, visit www.agilent.com/chem/education

LC AND LC/MS COLUMNS

823

Columns for Analytical HPLC

Superficially porous particles provide similar performance to sub-2 µm particles
This Van Deemter curve shows that Poroshell 120 –
a superficially porous, 2.7 µm particle column – can
deliver reduced plate heights similar to a 1.8 µm
column for similar efficiency.

10

9

Agilent Poroshell 120 EC-C18
3.0 x 100 mm, 2.7 µm
(USCFX01009)
P/N 695975-302

8

7

6

Agilent ZORBAX Eclipse Plus C18
3.0 x 100 mm, 1.8 µm
(USUYB01455)
P/N 959964-302

5

4

Agilent ZORBAX Eclipse Plus C18
3.0 x 100 mm, 3.5 µm
(USUXV01435)
P/N 959961-302

3

2

1

0

0

2

4

6

8

10

12

µ (mm/s)

UHPLC efficiency at HPLC pressures

2.223

0.826

LC AND LC/MS

9.753

6.273

4.132

2.816

1.518

2.016

1.148
0.646

2.705

2.5

824

5

7.5

10

B Agilent Eclipse Plus C18, 3.0 x 100 mm, 1.8 µm
PN 959964-302

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

N = 25053, Press = 182 bar

5

l 01357 522 961

7.5

13.041

8.21 8

5.281

3.494

2.418

For this sample of neutral alkylphenones,
the Poroshell 120 column delivered >90%
of the efficiency attained by the 1.8 µm column.
Also note that the pressure on the Poroshell 120
column is about 50% of the pressure on the
1.8 µm column.

N = 25053, Press = 182 bar

2.5

1.756

Sample:

60% Acetonitrile:40% Water
0.58 mL/min
60°C
4 µL
DAD Sig = 254,4 nm
Ref = 360,100 nm
(PN 5188-6529) spiked w/50 µL
2 mg/mL Thiourea in
water/acetonitrile (65:35)

0.865

Mobile Phase:
Flow Rate:
Temperature:
Injection Volume:
Detector:

A Agilent Poroshell 120 EC-C18, 3.0 x 100 mm, 2.7 µm
PN 695975-302

1.275

Column B:

Poroshell 120 EC-C18
695975-302
3 x 100 mm, 2.7 µm
Eclipse Plus C18
959964-302
3.0 x 100 mm, 1.8 µm

0.649

Column A:

10

12.5

min

Columns for Analytical HPLC

The simpler the manufacturing process, the more consistent the column
A single-step shell process creates a highly
reproducible column, as you can see in this
lot-to-lot comparison.

Agilent Poroshell 120 EC-C18
4.6 x 100 mm, 2.7 µm
P/N 695975-902 – from 4 Different Lots

B10014
0.5

1

1.5

2

2.5

3

0.5

1

1.5

2

2.5

3

0.5

1

1.5

2

2.5

3

0.5

1

1.5

2

2.5

3

min

B10015

B10016

B10018

Poroshell 120 EC-C8 is less retentive
for faster analysis of non-polar compounds
Column A:
Column B:

Mobile Phase:
Flow Rate:
Temperature:
Detector:
Sample:

Poroshell 120 EC-C18
699975-302
3 x 50 mm, 2.7 µm
Poroshell 120 EC-C8
699975-306
3.0 x 50 mm, 2.7 µm
60% CH3CN:40% H2O
0.85 mL/min
26°C
254 nm
2 µL of RRLC Checkout Sample
(PN 5188-6529), alkylphenones

A
mAU
300
250
200
150
100
50
0

0.5

1

1.5

2

2.5

3

3.5

min

0.5

1

1.5

2

2.5

3

3.5

min

B
mAU
300
250
200
150
100
50
0

LC AND LC/MS COLUMNS

825

Columns for Analytical HPLC

USP method for Naproxen tablets –
4.5X faster analysis on Agilent Poroshell 120
at HPLC pressures
Mobile Phase:
Flow Rate:

This Naproxen separation demonstrates how easy it can be to convert a method to Poroshell 120
columns without changing the flow rate or mobile phase.

50:49:1 MeCN:H2O
Acetic Acid
1.2 mL/min

4.6 x 150 mm (L1)
Agilent Eclipse Plus C18, 5 µm
P/N 959993-902
20 µL injection
Rs = 14.9
N=14,885

mAU
30

1. Naproxen
2. Butyrophenone

25

N=12,554

20
15
10
5
0

1

2

3

4

5

2X Faster
mAU
30

N=20,676

20

7

8

9

min

4.6 x 100 mm (L1)
Agilent Poroshell 120 EC-C18, 2.7 µm
P/N 695975-902
13.67 µL injection
Rs = 17.0
P = 238 bar

N=21,046

25

6

15
10
5
0

1

2

3

4

5

4.5X Faster
mAU
30

N=12,051

20

7

min

4.6 x 50 mm (L1)
Agilent Poroshell 120 EC-C18, 2.7 µm
P/N 699975-902
6.7 µL injection
Rs = 12.6
P = 133 bar

N=11,281

25

6

15
10
5
0

1

2

Agilent Poroshell 120 columns in series
deliver the highest efficiency at HPLC
and UHPLC pressures
Because low backpressure is one of the advantages of
Poroshell 120 columns, you can couple several columns
in series to achieve the highest separation power per unit
time. This enables better separation of complex samples.

3

4

5

6

7

min

3 Agilent Poroshell 120 EC-C18 columns in series
693975-902
4.6 x 150 mm, 2.7 µm

mAU
17.5
15

Flow Rate: 1 mL/min
N~83000 (peak 4)
Max Pressure: 316 bar

12.5
10
7.5
5
2.5

Peak #
2
3
4

Compound
Acetophenone
Benzene
Touene

Plates
114120
109931
114800

k’
0.29
0.46
0.65

0

0

1

2

3

4

5

6

min

2

3

4

5

6

min

mAU
17.5
15

Flow Rate: 1.5 mL/min
N~103000 (peak 4)
Max Pressure: 478 bar

12.5
10
7.5
5
2.5
0

0

1

mAU
17.5
15

Maximum efficiency
at 1.8 mL/min

Flow Rate: 1.8 mL/min
N~115000 (peak 4)
Max Pressure: 573 bar

12.5
10
7.5
5
2.5
0

0

1

2

3

4

5

6

min
LC2011_120

826

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

ZORBAX Eclipse Plus
• Excellent peak shape for basic compounds
• High level of performance – peak shape, efficiency, resolution, and lifetime –
with all sample types: acids, bases and neutrals
• Superior reproducibility with more rigorous QA/QC testing
• Improved, patented silica manufacturing with start-to-finish product control
• Available in 1.8, 3.5 and 5 µm particle sizes for all analytical, high resolution,
and fast LC analyses

Agilent ZORBAX Eclipse Plus columns provide the ultimate in performance for silica-based
columns. Peak shape is excellent for the most challenging basic compounds, improving efficiency
and resolution with these sample types. These results are achieved by improvements in the silica
manufacturing and bonding technology, which is completely controlled by Agilent.
Because of their high level of performance, Eclipse Plus columns are the ideal first choice for
method development of all samples. If you need to achieve fast method development and
superior productivity, then choose a column with high-resolution 1.8 µm particles. For standard
methods, conventional 5 µm and Rapid Resolution 3.5 µm columns are your best choice. With all
particle sizes, easy method transfer is possible.
With more rigorous QA and QC testing, column lot-to-lot reproducibility is also improved,
resulting in long-term reliable results for all analyses.

Column Specifications
Pore
Bonded Phase
Size
ZORBAX Eclipse Plus C18 95Å
ZORBAX Eclipse Plus C8 95Å
ZORBAX Eclipse PAH
95Å
ZORBAX Eclipse Plus
95Å
Phenyl-Hexyl

Surface
Area
160 m2/g
160 m2/g
160 m2/g
160 m2/g

Temp.
Limits
60°C
60°C
60°C
60°C

pH
Range*
2.0-9.0
2.0-9.0
2.0-8.0
2.0-8.0

Endcapped
Double
Double
No
Double

Carbon
Load
9%
7%
14%
9%

Specifications represent typical values only.
*Column lifetime will be reduced significantly at pH >7 and temperature >40°C. At pH 6-9, highest column stability for all silica
based columns is obtained by operating at temperatures <40°C and using lower buffer concentrations in range of 0.01-0.02 M,
especially with phosphate and carbonate buffers.

LC AND LC/MS COLUMNS

827

Columns for Analytical HPLC

ZORBAX Eclipse Plus: Best Peak Shape
in the Industry Without Tailing
Column:

Eclipse Plus C18
959996-902
4.6 x 100 mm, 5 µm

Mobile Phase:

A: 60% Water
B: 40% Acetonitrile
1.0 mL/min
Ambient
UV 254 nm
5989-4934EN
Pyridine, Phenol

Flow Rate:
Temperature:
Detector:
Publication:
Sample:

1

1. Pyridine
2. Phenol
2

Eclipse Plus C18
Ace 5 C18
SunFire C18
XBridge C18
Discovery HS C18
Luna C18 (2)
Gemini C18
XTerra MS C18
0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

Time (min)
LCEC001

Peak Shape and Efficiency
are Better with ZORBAX Eclipse Plus
Column A:
Column B:

XBridge C18, 4.6 x 150 mm, 5 µm
Eclipse Plus C18
959993-902
A
4.6 x 150 mm, 5 µm

Mobile Phase:

A: 0.1% formic acid
B: 0.1% formic acid in ACN
1.0 mL/min
0.0 min 10% B
15 min 30% B
40°C
UV 254 nm
5989-4934EN
Sulfonamides

Flow Rate:
Gradient:
Temperature:
Detector:
Publication:
Sample:

1

N = 14000
Tf = 1.25
3

2

0

2

B

1

828

LC AND LC/MS

2

5

4

4

6
Time (min)

4

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

1.
2.
3.
4.
5.
6.

6

8

10

N = 23000
Tf = 1.01
2

0

N = 41000
Tf = 1.13

12

N = 60000
Tf = 1.00
3

4

6
Time (min)

6

5

8

l 01357 522 961

10

12
LCEC003

Sulfanilamide
Sulfadiazine
Sulfathiazole
Sulfamerazine
Sulfmethazine
Sulfamethoxazole

Columns for Analytical HPLC

Eliminate Tailing and Maximize Resolution
with Eclipse Plus Columns
Column A:
Column B:

Eclipse Plus C18, 4.6 x 50 mm
Competitive C18, 4.6 x 50 mm

Mobile Phase:

65% ACN:35% 25 mM phosphate buffer
(pH 7.4)

1
Eclipse Plus C18, 4.6 x 50 mm

Tf = 1.20

1. Nortriptyline
2. Dipropylthalate

Competitive C18, 4.6 x 50 mm

1
Superior peak shape and better selectivity with
Eclipse Plus means more resolution, easier
quantitation and better results in your separations.

Tf = 4.86

2
2
0

0.5

1

1.5

2

min

Tips & Tools
To learn more about Agilent's complete portfolio of services,
please visit www.agilent.com/chem/services

LC AND LC/MS COLUMNS

829

Columns for Analytical HPLC

ZORBAX Eclipse Plus
Hardware Description
Analytical
Analytical
Analytical
Analytical
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Solvent Saver
Solvent Saver
Solvent Saver Plus
Solvent Saver Plus
Solvent Saver RRHD, 1200 bar
Solvent Saver RRHD, 1200 bar
Solvent Saver RRHD, 1200 bar
Solvent Saver HT, 600 bar
Solvent Saver HT, 600 bar
Narrow Bore
Narrow Bore
Narrow Bore
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RRHD, 1200 bar
Narrow Bore RRHD, 1200 bar
Narrow Bore RRHD, 1200 bar
Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
Guard Cartridges, 4/pk
Guard Cartridges, 4/pk
Guard Hardware Kit

830

LC AND LC/MS

Size (mm)
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 50
4.6 x 150
4.6 x 100
4.6 x 75
4.6 x 50
4.6 x 30
4.6 x 100
4.6 x 75
4.6 x 50
4.6 x 30
3.0 x 250
3.0 x 150
3.0 x 150
3.0 x 100
3.0 x 150
3.0 x 100
3.0 x 50
3.0 x 100
3.0 x 50
2.1 x 250
2.1 x 150
2.1 x 50
2.1 x 150
2.1 x 100
2.1 x 50
2.1 x 30
2.1 x 150
2.1 x 100
2.1 x 50
2.1 x 100
2.1 x 50
2.1 x 30
4.6 x 12.5
2.1 x 12.5

Particle Size
(µm)
5
5
5
5
3.5
3.5
3.5
3.5
3.5
1.8
1.8
1.8
1.8
5
5
3.5
3.5
1.8
1.8
1.8
1.8
1.8
5
5
5
3.5
3.5
3.5
3.5
1.8
1.8
1.8
1.8
1.8
1.8
5
5

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Eclipse
Plus C18
USP L1
959990-902
959993-902
959996-902
959946-902
959963-902
959961-902
959933-902
959943-902
959936-902
959964-902
959951-902
959941-902
959931-902

Eclipse
Plus C8
USP L7
959990-906
959993-906
959996-906
959946-906
959963-906
959961-906
959933-906
959943-906
959936-906
959964-906

Eclipse Plus
Phenyl-Hexyl
USP L11
959990-912
959993-912
959996-912

Eclipse
PAH
USP L1
959990-918
959993-918
959996-918

959963-912
959961-912
959933-912
959943-912
959936-912
959964-912

959963-918
959961-918

959941-906
959931-906

959941-912
959931-912

959941-918
959931-918
959990-318

959993-302
959963-302
959961-302
959759-302
959758-302
959757-302
959964-302
959941-302

959993-306
959963-306
959961-306
959759-306
959758-306
959757-306
959964-306
959941-306

959701-902
959746-902
959763-902
959793-902
959743-902
959733-902
959759-902
959758-902
959757-902
959764-902
959741-902
959731-902
820950-936
821125-936
820999-901

959701-906
959746-906
959763-906
959793-906
959743-906
959733-906
959759-906
959758-906
959757-906
959764-906
959741-906
959731-906
820950-937
821125-937
820999-901

l 01357 522 961

959943-918
959964-918

959963-312
959961-312

959964-312
959941-312
959701-912
959763-912
959793-912
959743-912
959733-912

959764-912
959741-912
959731-912
820950-938
821125-938
820999-901

959790-918
959701-918

959793-918

959764-918
959741-918
820950-939
821125-939
820999-901

Columns for Analytical HPLC

ZORBAX Eclipse XDB

CH3
O

Si
CH3
CH3
Si

O

CH3

CH3
CH3
O

Si
CH3
CH3

O

Si

CH3

CH3
CH3
O

• Four selectivity choices for method development optimization
• Good peak shape for basic, acidic and neutral compounds
• High performance over a wide pH range – pH 2-9
• Particle sizes from 1.8 µm to 7 µm
• Long lifetime with extra dense bonding and double endcapping

Si
CH3

eXtra Densely Bonded and Double Endcapped
Eclipse XDB Bonded Phase

Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase
choices for method development optimization. These columns provide good peak shape over a
wide pH range (2-9) for additional method development flexibility with one family of columns.
Eclipse XDB columns can be used for method development at low pH (2-3) and the same
column can be used for method development in the mid pH (6-8) region. In the mid pH region
residual silanols are more active and tailing interactions are more likely. To overcome these
interactions, Eclipse XDB columns are eXtra Densely Bonded and double endcapped through a
proprietary process to cover as many active silanols as possible. The result is superior peak shape
of basic compounds from pH 2-9. Eclipse XDB columns are available in 1.8, 3.5, 5 and 7 µm
particle sizes for high speed, high resolution, analytical and prep scale separations.

Column Specifications
Bonded Phase
ZORBAX Eclipse XDB-C18
ZORBAX Eclipse XDB-C8
ZORBAX Eclipse
XDB-Phenyl
ZORBAX Eclipse XDB-CN

Pore
Size
80Å
80Å
80Å
80Å

Temp.
pH
Carbon
Surface Area Limits Range* Endcapped Load
60°C 2.0-9.0
Double
10%
180 m2/g
60°C 2.0-9.0
Double
7.6%
180 m2/g
60°C 2.0-9.0
Double
7.2%
180 m2/g
180 m2/g

60°C

2.0-8.0

Double

4.3%

Specifications represent typical values only.
*Eclipse XDB columns are designed for operation over a wide pH range.At pH 6-9, highest columns stability for all silica based
columns is achieved by operating at temperatures <40°C and using low buffer concentrations in the range of 0.01-0.02 M.

LC AND LC/MS COLUMNS

831

Columns for Analytical HPLC

Good Peak Shape Over a Wide pH Range
with ZORBAX Eclipse XDB
Column:

Mobile Phase:

Flow Rate:
Temperature:

Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm

A

A: pH 3.0 75% 25 mM phosphate buffer
25% ACN
B: pH 7.0 90% 20 mM phosphate
10% ACN
1.5 mL/min
40°C

ZORBAX Eclipse XDB columns provide good peak
shape over a wide pH range and are an excellent
choice for method development from pH 2-9.

B

2

2

Sample:

3
1
3

4
5

B:
1. Procainamide
2. N-acetylprocainamide
3. N-propionylprocainamide

1

0

2.5

5.0
Time (min)

A:
1. Maleate
2. Doxylamine
3. Chlorpheniramine
4. Triprolidine
5. Diphenhydramine

7.5

0

2.5

5.0
Time (min)

7.5
LCEC004

Column Stability Testing at pH 3 and 60°C

Column:

Mobile Phase:

Flow Rate:
Temperature:
Sample:

ZORBAX SB-C8
883975-906
4.6 x 150 mm, 5 µm
Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
Purge Conditions:
70% 50 mM NaAc-HCl, pH 3.0
30% ACN
Retention Test Conditions:
65% Methanol
35% Water
1.0 mL/min
60°C
Tricyclic Antidepressants

7.0

6.5
K of Amitriptyline

Column:

6.0

ZORBAX SB-C8
5.5

ZORBAX Eclipse XDB-C8
Competitive C8

5.0
0

2

4
6
8
Column Volumes of Mobile Phase (X1000)

10

12
LCEC005

Eclipse XDB columns are stable over a wide pH range. At low pH an Eclipse endcapped column is extremely stable and shows equivalent stability to a nonendcapped column, SB-C8, at pH 3. The columns were purged with a pH 3 mobile phase at 60°C. Then they were tested with a strongly basic compound to
determine if the endcapping or bonded phase had been hydrolyzed from the silica surface. The Eclipse XDB column was very stable, as shown by the consistency
of the retention of amitriptyline over the 12,000 column volumes of the test. Another endcapped column shows less stability under these same conditions.

832

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

Column Stability Testing at pH 7.0
Column A:
Column B:

Mobile Phase:
Flow Rate:
Temperature:
Sample:

Competitive C8
SIL-type
After 1826 Column Volumes
Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
Sol-type
After 1843 Column Volumes
60% ACN
40% 250 mM Phosphate Buffer, pH 7.0
1.5 mL/min
60°C
Tricyclic Antidepressants

A

B

1
5

5

1

1.
2.
3.
4.
5.

2, 3

4

Uracil
Nortriptyline
Doxepin
Amitriptyline
Trimipramine

2

3
4

0

0 1 2 3 4 5 6 7 8 9 10 11 12
Time (min)

1

2

3

4
5
Time (min)

6

7

8
LCEC006

Double endcapping, dense bonding and the durable Rx-Sil particles (sol-type) combine to provide long lifetime at pH 7 when compared to single endcapped sil-gel
columns used here. The conditions used for this test – high temperature (60°C) and high salt concentration (250 mM), accelerate the dissolution of silica, causing
premature failure of the sil-gel type column.

Selectivity Changes for Basic Compounds
with Eclipse XDB and StableBond
Column A:
Column B:

Mobile Phase:
Flow Rate:
Temperature:

A

Eclipse XDB-C8
966967-906
4.6 x 75 mm, 3.5 µm
ZORBAX Rx/SB-C8
866953-906
4.6 x 75 mm, 3.5 µm

2

1. Barbital
2. Sulfamethoxazole
3. Caffeine

3

70% 25 mM NaH2PO4, pH 3.0
30% Methanol
1.0 mL/min
35°C

Eclipse XDB and StableBond columns are based on the same
silica but have different bonding and endcapping. Therefore,
they can have very different selectivity for the same sample
under the same conditions, as this example shows.

B

2

3

1

1

0

0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
Time (min)

0

0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
Time (min)
LCEC008

LC AND LC/MS COLUMNS

833

Columns for Analytical HPLC

Optimize Separations with Eclipse XDB
Selectivity Options: Analysis of Sunscreens
Column A:
Column B:
Column C:

Eclipse XDB-Phenyl
963967-912
4.6 x 150 mm, 3.5 µm
Eclipse XDB-C8
963967-906
4.6 x 150 mm, 3.5 µm
Eclipse XDB-C18
963967-902
4.6 x 150 mm, 3.5 µm

A

1

1. Oxybenzone
2. Padimate-O
3. Ethylhexylsalicylate

2
3

Mobile Phase:
Flow Rate:
Temperature:
Sample:

0
B

15% H2O:85% MeOH
1.0 mL/min
35°C
Sunscreens

2

4

6

8

10

12

14

16

10

12

14

16

1

2
3

0
C

This separation of sunscreens on all three Eclipse XDB bonded phases – C18, C8 and
Phenyl – shows that different bonded phases can be used to optimize a separation. While
all three bonded phases provide an adequate separation, the Eclipse XDB-Phenyl provides
a different peak elution order and a much shorter overall analysis time. All three bonded
phases also provide excellent peak shape with no mobile phase additives.

2

4

6

8

1

2
3

0

2

4

6

8
10
Time (min)

12

14

LCEC007

Selectivity for Urea Pesticides
Column A:
Column B:
Column C:

Mobile Phase:

Flow Rate:
Temperature:
Sample:

Eclipse XDB-C18
993967-902
4.6 x 150 mm, 5 µm
Eclipse XDB-CN
993967-905
4.6 x 150 mm, 5 µm
Eclipse XDB-C18
993967-902
4.6 x 150 mm, 5 µm
A. 60:40 MeOH:Water
B. 60:40 MeOH:Water
C. 77:23 MeOH:Water
1.0 mL/min
25°C
Urea pesticides

A

2
3

4

1

5
6

0

10

B

30
Time (min)

40

50

2
34

5

1

0

2

C

2

6

4
6
Time (min)
34

2

8

10

8

10

5

1

0

20

1.
2.
3.
4.
5.
6.

Fenuron
Monuron
MonoLinuron
Diuron
Linuron
Pencycuron

6

4
6
Time (min)

The Eclipse XDB-CN column reduces retention time and provides good selectivity for Urea pesticides when compared to an Eclipse XDB-C18 column.

834

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

16

LCEC010

Columns for Analytical HPLC

ZORBAX Eclipse XDB
Hardware Description

Size (mm) Particle Size (µm)

Eclipse
XDB-C18
USP L1

Eclipse
XDB-C8
USP L7

990967-202
990967-902
993967-902
946975-902
963967-902
961967-902
966967-902
935967-902
934967-902
932967-902
928975-902
927975-902
924975-902
926975-902
990967-302
993967-302
963954-302
961967-302
966954-302
981759-302
981758-302
981757-302
928975-302
927975-302
924975-302
926975-302
993700-902
960967-902
930990-902
961753-902
966735-902
971700-902
974700-902
972700-902
981759-902
981758-902
981757-902

990967-206
990967-906
993967-906
946975-906
963967-906
961967-906
966967-906
935967-906
934967-906
932967-906
928975-906
927975-906
924975-906
926975-906
990967-306
993967-306
963954-306
961967-306

Eclipse
XDB-Phenyl
USP L11

Eclipse
XDB-CN
USP L10

990967-912
993967-912

990967-905
993967-905

963967-912

963967-905
961967-905
966967-905

Standard Columns (no special hardware required)

Semi-Preparative
Analytical
Analytical
Analytical
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Solvent Saver
Solvent Saver
Solvent Saver Plus
Solvent Saver Plus
Solvent Saver Plus
Solvent Saver RRHD, 1200 bar
Solvent Saver RRHD, 1200 bar
Solvent Saver RRHD, 1200 bar
Solvent Saver HT, 600 bar
Solvent Saver HT, 600 bar
Solvent Saver HT, 600 bar
Solvent Saver HT, 600 bar
Narrow Bore
Narrow Bore
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RRHD, 1200 bar
Narrow Bore RRHD, 1200 bar
Narrow Bore RRHD, 1200 bar

9.4 x 250
4.6 x 250
4.6 x 150
4.6 x 50
4.6 x 150
4.6 x 100
4.6 x 75
4.6 x 50
4.6 x 30
4.6 x 20
4.6 x 100
4.6 x 50
4.6 x 30
4.6 x 20
3.0 x 250
3.0 x 150
3.0 x 150
3.0 x 100
3.0 x 75
3.0 x 150
3.0 x 100
3.0 x 50
3.0 x 100
3.0 x 50
3.0 x 30
3.0 x 20
2.1 x 150
2.1 x 50
2.1 x 150
2.1 x 100
2.1 x 75
2.1 x 50
2.1 x 30
2.1 x 20
2.1 x 150
2.1 x 100
2.1 x 50

5
5
5
5
3.5
3.5
3.5
3.5
3.5
3.5
1.8
1.8
1.8
1.8
5
5
3.5
3.5
3.5
1.8
1.8
1.8
1.8
1.8
1.8
1.8
5
5
3.5
3.5
3.5
3.5
3.5
3.5
1.8
1.8
1.8

928975-306
927975-306
924975-306
926975-306
993700-906
960967-906
930990-906
961753-906

966967-912
935967-912

990967-312
993967-312
963954-312
961967-312

990967-305
993967-305
963954-305

993700-912
960967-912

993700-905
960967-905
961753-905

971700-906
974700-906
972700-906

(Continued)

LC AND LC/MS COLUMNS

835

Columns for Analytical HPLC

ZORBAX Eclipse XDB
Hardware Description

Eclipse
XDB-C18
USP L1

Eclipse
XDB-C8
USP L7

Eclipse
XDB-Phenyl
USP L11

Eclipse
XDB-CN
USP L10

1.8
1.8
1.8
1.8
3.5
3.5
3.5
5
5
5

928700-902
927700-902
924700-902
926700-902
963600-902
965600-902
961600-902
5185-5921
820950-925
821125-926
820999-901

928700-906
927700-906
924700-906
926700-906
963600-906
965600-906
961600-906
5185-5921
820950-926
821125-926
820999-901

820950-927
821125-926
820999-901

820950-935
821125-935
820999-901

Size (mm) Particle Size (µm)

Standard Columns (no special hardware required)

Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
MicroBore RR
MicroBore RR
MicroBore RR
MicroBore Guard, 3/pk
Guard Cartridges, 4/pk
Guard Cartridges, 4/pk
Guard Hardware Kit

2.1 x 100
2.1 x 50
2.1 x 30
2.1 x 20
1.0 x 150
1.0 x 50
1.0 x 30
1.0 x 17
4.6 x 12.5
2.1 x 12.5

PrepHT Cartridge Columns (require endfittings kit 820400-901)

PrepHT Cartridge

21.2 x 250

7

977250-102

977250-106

PrepHT Cartridge

21.2 x 150

7

977150-102

977150-106

PrepHT Cartridge

21.2 x 150

5

970150-902

970150-906

PrepHT Cartridge

21.2 x 100

5

970100-902

970100-906

PrepHT Cartridge

21.2 x 50

5

970050-902

970050-906

PrepHT Guard Cartridge

17 x 7.5

5

820212-925

820212-926

Guard Cartridge Hardware

820444-901

820444-901

PrepHT endfittings, 2/pk

820400-901

820400-901

Unless indicated, column pressure limit is 400 bar.

ZORBAX Eclipse XDB
Size (mm) Particle Size (µm)

Eclipse
XDB-C18
USP L1

Eclipse
XDB-C8
USP L7

Analytical

4.6 x 250

5

7995118-585

7995108-585

Analytical

4.6 x 150

5

7995118-595

7995108-595

Rapid Resolution

4.6 x 75

3.5

7995118-344

7995108-344

Solvent Saver Plus

3.0 x 75

3.5

7995230-344

Guard Cartridges, 10/pk
Cartridge Holder, 5021-1845

4.0 x 4

5

7995118-504
5021-1845

Hardware Description
Agilent Cartridge Columns (require hardware kit 5021-1845)

7995118-504
5021-1845
(Continued)

836

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

ZORBAX Eclipse XDB
Size (mm) Particle Size (µm)

Eclipse
XDB-C18
USP L1

Eclipse
XDB-C8
USP L7

4.6 x 50
4.6 x 50
2.1 x 50
2.1 x 50

1.8
1.8
1.8
1.8

922975-902
922975-932
922700-902
922700-932

922975-906

Rapid Resolution Cartridge

4.6 x 30

3.5

933975-902

933975-906

Rapid Resolution Cartridge, 3/pk

4.6 x 30

3.5

933975-932

933975-936

Rapid Resolution Cartridge

4.6 x 15

3.5

931975-902

931975-906

Rapid Resolution Cartridge, 3/pk

4.6 x 15

3.5

931975-932

931975-936

Rapid Resolution Cartridge

2.1 x 30

3.5

973700-902

973700-906

Rapid Resolution Cartridge, 3/pk

2.1 x 30

3.5

973700-932

973700-936

Rapid Resolution Cartridge

2.1 x 15

3.5

975700-902

975700-906

Rapid Resolution Cartridge, 3/pk

2.1 x 15

3.5

975700-932

975700-936

Rapid Resolution HT Cartridge

4.6 x 50

1.8

925975-902

Rapid Resolution HT Cartridge, 3/pk

4.6 x 50

1.8

925975-932

Rapid Resolution HT Cartridge

4.6 x 30

1.8

923975-902

Rapid Resolution HT Cartridge, 3/pk

4.6 x 30

1.8

923975-932

Rapid Resolution HT Cartridge

4.6 x 15

1.8

921975-902

Rapid Resolution HT Cartridge, 3/pk

4.6 x 15

1.8

921975-932

Rapid Resolution HT Cartridge

2.1 x 50

1.8

925700-902

Rapid Resolution HT Cartridge, 3/pk

2.1 x 50

1.8

925700-932

Rapid Resolution HT Cartridge

2.1 x 30

1.8

923700-902

Rapid Resolution HT Cartridge, 3/pk

2.1 x 30

1.8

923700-932

Rapid Resolution HT Cartridge

2.1 x 15

1.8

921700-902

Rapid Resolution HT Cartridge, 3/pk

2.1 x 15

1.8

Hardware Description
Standard Columns (no special hardware required)

Rapid Resolution HT
Rapid Resolution HT, 3/pk
Narrow Bore RRHT
Narrow Bore RRHT, 3/pk
Rapid Resolution HT Cartridges (require hardware kit 820555-901)

Hardware Kit for RR and RRHT Cartridges

921700-932
820555-901

Capillary Glass-lined Columns

Capillary
Capillary
Capillary RR
Capillary RR
Capillary
Capillary
Capillary RR
Capillary
Capillary

0.5 x 250
0.5 x 150
0.5 x 150
0.5 x 35
0.3 x 250
0.3 x 150
0.3 x 150
0.5 x 35
0.3 x 35

5
5
3.5
3.5
5
5
3.5
5
5

5064-8286
5064-8287
5064-8288
5064-8298
5064-8269
5064-8291
5064-8271
5064-8296
5064-8297

LC AND LC/MS COLUMNS

837

Columns for Analytical HPLC

ZORBAX 80Å StableBond
R
O

Si

R1

without degradation
• Six different bonded phases provide broad selectivity – SB-C18, SB-C8, SB-CN, SB-Phenyl,
SB-C3, and SB-Aq
• High purity (Type B) silica for good peak shape

R
OH
R
O

Si

R1

R
OH
R
O

Si

• Longest column lifetime and best reproducibility for low pH separations – down to pH 1
• Patented stable column chemistry allows use at high temperature and low pH

R1

R

Agilent ZORBAX StableBond columns use patented, unique, nonfunctional silanes with bulky
diisobutyl (SB-C18) or diisopropyl (SB-C8, SB-C3, SB-Phenyl, SB-CN, and SB-Aq) side chain
groups that sterically protect the key siloxane bond to the silica surface from hydrolytic attack at
low pH. StableBond packing materials are not endcapped in order to provide exceptional stability
and to maximize lifetime and reproducibility under acidic mobile phase conditions. The high
purity, low acidity silica provides excellent peak shape with acidic, basic and neutral compounds
making StableBond columns an excellent choice for low pH method development. ZORBAX
StableBond columns are compatible with all common mobile phases, including very high
aqueous mobile phases.

Column Specifications

Sterically Protected StableBond Bonded Phase

Bonded Phase
ZORBAX SB-C18
ZORBAX SB-C8
ZORBAX SB-C3
ZORBAX SB-Phenyl
ZORBAX SB-CN
ZORBAX SB-Aq

Pore
Size
80Å
80Å
80Å
80Å
80Å
80Å

Surface
Area
180 m2/g
180 m2/g
180 m2/g
180 m2/g
180 m2/g
180 m2/g

Temp.
Limits*
90°C
80°C
80°C
80°C
80°C
80°C

pH Range* Endcapped
0.8-8.0
No
1.0-8.0
No
1.0-8.0
No
1.0-8.0
No
1.0-8.0
No
1.0-8.0
No

Carbon
Load
10%
5.5%
4%
5.5%
4%
proprietary

Specifications represent typical values only.
*StableBond columns are designed for optimal use at low pH. At pH 6-8, highest column stability for all silica-based columns is
obtained by operating at temperatures <40°C and using lower buffer concentrations in the range of 0.01-0.02 M. At mid-range
pH, Eclipse Plus, Eclipse XDB and Bonus-RP are recommended.

838

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

StableBond SB-C18 Shows Excellent
Stability at Low pH and High Temperature
(pH 0.8, 90°C)
Column:
Column:

Mobile Phase:

Temperature:

ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
ZORBAX Rx-C18
883967-902
4.6 x 150 mm, 5 µm

As an indicator of column breakdown, retention time of toluene was measured after purging the column
with mobile phase. Only the StableBond SB-C18 is unchanged after three working months of use under
these very low pH (0.8) and high temperature (90°C) conditions. ZORBAX Rx-C18 also provides a stable
matrix, and can be used as an alternative selectivity to StableBond SB-C18.

50% Methanol/50% Water
with 1.0% TFA
Test Solute: Toluene
90°C

StableBond SB-C18 (Diisobutyl-C18)

Relative Retention

1.0

ZORBAX Rx-C18 (Dimethyl-C18)

0.8

Competitor A-C18

0.6

Competitor B-C18
Competitor C-C18

0.4

Competitor D-C18
0.2

Competitor E-C18

0.0
0

5,000

10,000

15,000

20,000

25,000

30,000

Column Volumes of Mobile Phase Purge
LCSB001

Shorter Chain ZORBAX SB-CN is also Stable
at Low pH (pH 2.0, 50°C)
ZORBAX SB-CN
883975-905
4.6 x 150 mm, 5 µm

Mobile Phase:
Flow Rate:
Gradient:
Temperature:
Sample:

0.1% TFA, pH 2:ACN
1 mL/min
0-100% ACN
50°C
1-phenylheptane @ 50% AC/50% Water
with 0.1% TFA

SB-CN

100

80

% k Remaining

Column:

60

40
Si-(Me)2PrCN
20

ZORBAX StableBond SB-CN and other short chain
StableBond bonded phases are also exceptionally
stable at low pH. Conventional dimethyl CN and similar
bonded phases lack this stability.

0
0

1,000

2,000

3,000

4,000

5,000

6,000

Column Volumes
LCSB002

LC AND LC/MS COLUMNS

839

Columns for Analytical HPLC

SB-CN Optimizes Retention and Resolution
Column A:
Column B:

Mobile Phase:
Flow Rate:
Temperature:

ZORBAX SB-C18
866953-902
4.6 x 75 mm, 3.5 µm
ZORBAX SB-CN
866953-905
4.6 x 75 mm, 3.5 µm

1.
2.
3.
4.
5.

A
2

30% ACN
70% 25mM NaH2PO4, pH 2.5
1.0 mL/min
35°C

Estriol
Daidzen
Quercetin
Genistein
Diethylstilbestrol

B
5

1
4

2

3

4

5
1

The SB-CN column is used here to reduce analysis time by
50%. The retention of the most hydrophobic analyte is cut in
half. At the same time retention of the more polar, early
eluting peaks increases slightly.

0

5

10
15
Time (min)

20

25

0

3

2

4

6
8 10
Time (min)

12
LCSB003

Five Different Bonded Phases Provide
Selectivity Options
Column A:
Column B:
Column C:
Column D:
Column E:

Mobile Phase:

Flow Rate:
Temperature:
Detector:
Sample:

ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
ZORBAX SB-C8
883975-906
4.6 x 150 mm, 5 µm
ZORBAX SB-C3
883975-909
4.6 x 150 mm, 5 µm
ZORBAX SB-Phenyl
883975-912
4.6 x 150 mm, 5 µm
ZORBAX SB-CN
883975-905
4.6 x 150 mm, 5 µm
0-100% B in 18.8 min
A: 50 mM NaH2PO4,
pH 2.5 in 95% H2O / 5% ACN
B: 50 mM NaH2PO4,
pH 2.5 in 47% H2O / 53% ACN
1.0 mL/min
26°C
254 nm
1. Procaine
2. Lidocaine
3. d-Cinchonine
4. Butacaine
5. Tetracaine

SB-C3 is just one of the five different StableBond selectivity choices. In this
example, optimum resolution is obtained with SB-C3. All are based on the same
high purity Rx-SIL. Selectivity changes are therefore dependent only on the
bonded phases, making method development more reliable.

A

3

1

5
4
2

3

B
1

5
4
2

3

C

1
5
4
2

3

D
1

5
4
2

E

13
5
4

2

0

5

10

15

Time (min)
LCSB004

840

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

ZORBAX 80Å StableBond
Hardware Description

Size (mm)

Particle
Size SB-C18
(µm) USP L1

SB-C8
USP L7

SB-CN
USP L10

SB-C3
USP L56

SB-Phenyl
USP L11

SB-Aq

Standard Columns (no special hardware required)

Semi-Preparative
Semi-Preparative
Semi-Preparative
Semi-Preparative
Analytical
Analytical
Analytical
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution HT,
600 bar
Rapid Resolution HT,
600 bar
Rapid Resolution HT,
600 bar
Rapid Resolution HT,
600 bar
Rapid Resolution HT,
600 bar
Solvent Saver
Solvent Saver
Solvent Saver Plus
Solvent Saver Plus
Solvent Saver Plus

9.4 x 250
9.4 x 150
9.4 x 100
9.4 x 50
4.6 x 250
4.6 x 150
4.6 x 50
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 75
4.6 x 50
4.6 x 30
4.6 x 20
4.6 x 150

5
5
5
5
5
5
5
3.5
3.5
3.5
3.5
3.5
3.5
3.5
1.8

880975-202
883975-202
884975-202
846975-202
880975-902
883975-902
846975-902
884950-567
863953-902
861953-902
866953-902
835975-902
834975-902
832975-902
829975-902

4.6 x 100

880967-201 880975-205 880975-209 880975-212

880975-906 880975-905 880975-909 880975-912 880975-914
883975-906 883975-905 883975-909 883975-912 883975-914
846975-906
846975-914
863953-906
861953-906
866953-906
835975-906
834975-906
832975-906
829975-906

863953-905
861953-905
866953-905
835975-905

863953-912
861953-912
866953-912
835975-912

829975-905

829975-912 829975-914

1.8

828975-902 828975-906 828975-905

828975-912 828975-914

4.6 x 50

1.8

827975-901 827975-906 827975-905

827975-912 827975-914

4.6 x 30

1.8

824975-902 824975-906 824975-905

824975-912 824975-914

4.6 x 20

1.8

826975-902 826975-906

3.0 x 250
3.0 x 150
3.0 x 150
3.0 x 100
3.0 x 75

5
5
3.5
3.5
3.5

880975-302
883975-302
863954-302
861954-302
866953-302

880975-306
883975-306
863954-306
861954-306

863953-914
861953-914
866953-914
835975-914

880975-305 880975-309 880975-312 880975-314
883975-305 883975-309 883975-312 883975-314
863954-305
863954-312 863954-314
861954-305 861954-309 861954-312 861954-314

Unless indicated, column pressure limit is 400 bar.

(Continued)

LC AND LC/MS COLUMNS

841

Columns for Analytical HPLC

ZORBAX 80Å StableBond
Hardware Description

Size (mm)

Particle
Size SB-C18
(µm) USP L1

SB-C8
USP L7

SB-CN
USP L10

SB-C3
USP L56

SB-Phenyl
USP L11

SB-Aq

Standard Columns (no special hardware required)

Solvent Saver RRHD,
1200 bar
Solvent Saver RRHD,
1200 bar
Solvent Saver RRHD,
1200 bar
Solvent Saver HT,
600 bar
Solvent Saver HT,
600 bar
Solvent Saver HT,
600 bar
Solvent Saver HT,
600 bar
Solvent Saver HT,
600 bar
Narrow Bore
Narrow Bore
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RRHD,
1200 bar
Narrow Bore RRHD,
1200 bar
Narrow Bore RRHD,
1200 bar

3.0 x 150

1.8

859700-302 859700-306

3.0 x 100

1.8

858700-302 858700-306 858700-305

858700-312

3.0 x 50

1.8

857700-302 857700-306 857700-305

857700-312

3.0 x 150

1.8

829975-302 829975-306 829975-305

829975-312

3.0 x 100

1.8

828975-302 828975-306 828975-305 828975-309 828975-312 828975-314

3.0 x 50

1.8

827975-302 827975-306 827975-305

3.0 x 30

1.8

824975-302 824975-306 824975-305

3.0 x 20

1.8

826975-302 826975-306

2.1 x 150
2.1 x 50
2.1 x 150
2.1 x 100
2.1 x 75
2.1 x 50
2.1 x 30
2.1 x 20
2.1 x 150

5
5
3.5
3.5
3.5
3.5
3.5
3.5
1.8

883700-922
860975-902
830990-902
861753-902
866735-902
871700-902
874700-902
872700-902
859700-902

871700-906
874700-906
872700-906
859700-906 859700-905

859700-912

2.1 x 100

1.8

858700-902 858700-906 858700-905

858700-912

2.1 x 50

1.8

857700-902 857700-906 857700-905

857700-912

827975-312 827975-314

883700-906 883700-905 883700-909 883700-912
860975-906 860975-905 860975-909 860975-912 860975-914
830990-906
830990-914
861753-906 861753-905
861753-912 861753-914
871700-914

Unless indicated, column pressure limit is 400 bar.

(Continued)

842

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

ZORBAX 80Å StableBond
Hardware Description

Size (mm)

Particle
Size SB-C18
(µm) USP L1

SB-C8
USP L7

SB-CN
USP L10

SB-C3
USP L56

SB-Phenyl
USP L11

SB-Aq

Standard Columns (no special hardware required)

Narrow Bore RRHT,
600 bar
Narrow Bore RRHT,
600 bar
Narrow Bore RRHT,
600 bar
Narrow Bore RRHT,
600 bar
Narrow Bore RRHT,
600 bar
MicroBore RR
MicroBore RR
MicroBore RR
MicroBore Guard, 3/pk
Guard Cartridge, 2/pk
Guard Cartridge, 4/pk
Guard Cartridge, 4/pk
Guard Hardware Kit
Guard Hardware Kit

2.1 x 150

1.8

820700-902 820700-906 820700-905

820700-912

2.1 x 100

1.8

828700-902 828700-906 828700-905

828700-912 828700-914

2.1 x 50

1.8

827700-902 827700-906 827700-905

827700-912 827700-914

2.1 x 30

1.8

824700-902 824700-906 824700-905

824700-912 824700-914

2.1 x 20

1.8

826700-902 826700-906

1.0 x 150
1.0 x 50
1.0 x 30
1.0 x 17
9.4 x 15
4.6 x 12.5
2.1 x 12.5
9.4 x 15

3.5
3.5
3.5
5
7
5
5
0

863600-902
865600-902
861600-902
5185-5920
820675-115
820950-920
821125-915
840140-901
820999-901

863600-906
865600-906
861600-906
5185-5920
820675-115
820950-915
821125-915
840140-901
820999-901

863600-905

820675-124
820950-916
821125-924
840140-901
820999-901

820675-124
820950-922
821125-924
840140-901
820999-901

820675-115
820950-917 820950-933
821125-915 821125-933
840140-901
820999-901 820999-901

PrepHT Cartridge Columns (require endfittings kit 820400-901)

PrepHT Cartridge

21.2 x 250

7

877250-102 877250-106 877250-105

877250-112 877250-114

PrepHT Cartridge

21.2 x 150

7

877150-102 877150-106

877150-114

PrepHT Cartridge

21.2 x 150

5

870150-902 870150-906

870150-914

PrepHT Cartridge

21.2 x 100

5

870100-902 870100-906

870100-914

PrepHT Cartridge

21.2 x 50

5

870050-902 870050-906

870050-914

PrepHT Guard
Cartridge, 2/pk
Guard Cartridge
Hardware
PrepHT endfittings,
2/pk

17 x 7.5

5

820212-920 820212-915 820212-915

820212-915 820212-933

820444-901 820444-901 820444-901 820444-901 820444-901 820444-901
820400-901 820400-901 820400-901 820400-901 820400-901 820400-901

Unless indicated, column pressure limit is 400 bar.

LC AND LC/MS COLUMNS

843

Columns for Analytical HPLC

ZORBAX 80Å StableBond
Hardware Description

Size (mm) Particle Size (µm)

SB-C18
USP L1

SB-C8
USP L7

SB-Phenyl
USP L11

Agilent Cartridge Columns (require hardware kit 5021-1845)

Analytical

4.6 x 250

5

7995218-585

7995208-585

Analytical

4.6 x 150

5

7995218-595

7995208-595

Rapid Resolution

4.6 x 75

3.5

7995218-344

7995208-344

Guard Cartridges, 10/pk

4.0 x 4

5

7995118-504

7995118-504

5021-1845

5021-1845
822975-906

Cartridge Holder, 5021-1845
Standard Columns (no special hardware required)

Rapid Resolution HT

4.6 x 50

1.8

822975-902

Rapid Resolution HT, 3/pk

4.6 x 50

1.8

822975-932

Narrow Bore RRHT

2.1 x 50

1.8

822700-902

Narrow Bore RRHT, 3/pk

2.1 x 50

1.8

822700-932

Rapid Resolution Cartridges (require hardware kit 820555-901)

Rapid Resolution Cartridge

4.6 x 30

3.5

833975-902

833975-906

Rapid Resolution Cartridge, 3/pk

4.6 x 30

3.5

833975-932

833975-936

Rapid Resolution Cartridge

4.6 x 15

3.5

831975-902

831975-906

Rapid Resolution Cartridge, 3/pk

4.6 x 15

3.5

831975-932

831975-936

Rapid Resolution Cartridge

2.1 x 30

3.5

873700-902

873700-906

Rapid Resolution Cartridge, 3/pk

2.1 x 30

3.5

873700-932

873700-936

Rapid Resolution Cartridge

2.1 x 15

3.5

875700-902

875700-906

Rapid Resolution Cartridge, 3/pk

2.1 x 15

3.5

875700-932

875700-936

833975-912

(Continued)

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

844

LC AND LC/MS

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www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

ZORBAX 80Å StableBond
Hardware Description

Size (mm) Particle Size (µm)

SB-C18
USP L1

SB-C8
USP L7

SB-Phenyl
USP L11

Rapid Resolution HT Cartridges (require hardware kit 820555-901)

Rapid Resolution HT Cartridge

4.6 x 50

1.8

825975-902

Rapid Resolution HT Cartridge, 3/pk

4.6 x 50

1.8

825975-932

Rapid Resolution HT Cartridge

4.6 x 30

1.8

823975-902

Rapid Resolution HT Cartridge, 3/pk

4.6 x 30

1.8

823975-932

Rapid Resolution HT Cartridge

4.6 x 15

1.8

821975-902

Rapid Resolution HT Cartridge, 3/pk

4.6 x 15

1.8

821975-932

Rapid Resolution HT Cartridge

2.1 x 50

1.8

825700-902

Rapid Resolution HT Cartridge, 3/pk

2.1 x 50

1.8

825700-932

Rapid Resolution HT Cartridge

2.1 x 30

1.8

823700-902

Rapid Resolution HT Cartridge, 3/pk

2.1 x 30

1.8

823700-932

Rapid Resolution HT Cartridge

2.1 x 15

1.8

821700-902

Rapid Resolution HT Cartridge, 3/pk

2.1 x 15

1.8

821700-932

Hardware Kit for RR and RRHT Cartridges

820555-901

ZORBAX 80Å StableBond
Description

Size (mm)

Particle Size (µm)

SB-C18
USP L1

Capillary Glass-lined Columns

Capillary
Capillary
Capillary
Capillary RR
Capillary RR
Capillary
Capillary
Capillary
Capillary RR

0.5 x 250
0.5 x 150
0.5 x 35
0.5 x 150
0.5 x 35
0.3 x 250
0.3 x 150
0.3 x 35
0.3 x 150

5
5
5
3.5
3.5
5
5
5
3.5

5064-8258
5064-8256
5064-8254
5064-8262
5064-8260
5064-8257
5064-8255
5064-8253
5064-8261

LC AND LC/MS COLUMNS

845

Columns for Analytical HPLC

ZORBAX Bonus-RP

R1
O

PG

Si

R

R1
CH3
Si
CH3
O

CH3
R1
PG

Si

R

R1
CH3
Si

CH3

The Agilent ZORBAX Bonus-RP column has a polar amide group embedded in a long alkyl chain.
This novel bonding reduces interactions between basic compounds and the silica support,
improving peak shape for the most difficult basic compounds. Peak shape and column lifetime
are further improved by triple endcapping. In addition, diisopropyl side groups provide steric
protection against acid hydrolysis for good lifetime at low pH. The Bonus-RP column provides an
alternate selectivity to C18 and C8 alkyl bonded phases.

Column Specifications

CH3
R1
O

• Excellent peak shape for challenging basic compounds at low and mid pH
• Unique reversed-phase selectivity
• Novel bonding technology with embedded polar group and steric protection
• Usable in 100% aqueous mobile phases

Si

PG

R

Bonded Phase
ZORBAX Bonus-RP

Pore
Size
80Å

Surface
Area
180 m2/g

Temp.
Limits*
60°C

pH Range
2.0-9.0

Endcapped
Triple

Carbon
Load
9.5%

Specifications represent typical values only.

R1

*Temperature limits are 60°C up to pH 8, 40°C from pH 8-9.

Unique, Polar Alkyl Bonus-RP Bonded Phase

Improved Peak Shape of Basic Compounds
Using Bonus-RP
Column:
Mobile Phase:
Flow Rate:
Temperature:
Detector:

1

Alkyl-C8
4.6 x 150 mm, 5 µm

2

TF
1. 1.8
2. 1.7
3. 2.1

75% 25 mM NH4OAc, pH 5.5
25% ACN
1.5 mL/min
40°C
254 nm

0

Column:

ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm

Mobile Phase:

80% 25 mM NH4OAc, pH 5.5
20% ACN
1.5 mL/min
40°C
254 nm

Flow Rate:
Temperature:
Detector:

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

3

2

3

4 5 6
Time (min)

7

8

9

10

LCBR001

3

TF
1. 1.1
2. 1.1
3. 1.1

Bonus-RP eliminates peak tailing of these basic compounds in comparison to a typical
alkyl C8 bonded phase. In the mid-pH region, residual silanols can interact more strongly
with basic compounds to cause peak tailing. The polar group in the Bonus-RP bonded
phase eliminates peak tailing of these basic compounds by reducing interactions with
residual silanols.

846

1

1. Doxylamine
2. Chlorpheniramine
3. Triprolidine

0

1. Doxylamine
2. Chlorpheniramine
3. Triprolidine

1

2

1

2

l 01357 522 961

3
4
Time (min)

5

6
LCBR002

Columns for Analytical HPLC

Column:

ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm

Mobile Phase:

60% 25 mM
Phosphate Buffer,
pH 7.0:40% ACN
1.5 mL/min
23°C

Flow Rate:
Temperature:

0.0018
Toluene Plate Height, cm

ZORBAX Bonus-RP is Stable
at Low and Mid pH

Competitive alkyl/amide
Bonus-RP

0.0016

0.0014

0.0012

0.0010
0

5,000

10,000

15,000

20,000

25,000

30,000

35,000

Column Volume of Purge
LCBR003

Triple endcapping of Bonus-RP enhances stability at pH 7. Each 10,000 column volume is equivalent to approximately one working month.

Dimethyl-C18/amide, Bonus-RP
ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm

Mobile Phase:

Aging:
50% MeOH
50% 0.1% TFA
Test:
80% MeOH
20% H2O
1.0 mL/min
Aging:
60°C
Test:
23°C

Flow Rate:
Temperature:

0.0055

Dimethyl-C18/amide
Bonus-RP

0.0050
Toluene Plate Height, cm

Column:

0.0045
0.0040
0.0035
0.0030
0.0025
0.0020
0

10,000

20,000

30,000

40,000

Column Volume of Purge
LCBR004

Sterically protecting side groups provide good low pH stability and longer column lifetime than similar polar alkyl bonded phases.

Tips & Tools
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LC AND LC/MS COLUMNS

847

Columns for Analytical HPLC

ZORBAX Bonus-RP Provides Unique
Selectivity
Column A:
Column B:

Mobile Phase:
Flow Rate:
Temperature:
Detector:
Sample:

ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm
Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm

1.
2.
3.
4.

A
1

mAu
15
10

75% 25 mM Na Citrate, pH 6
25% MeOH
1.0 mL/min
Ambient
254 nm
3 µL
Cephalosporins

Cephalexin
Cephaclor
Cephuroxime
Cephoxitin

3

5

2

4

0
0

2

4

6

8

10

Time (min)
B
1

mAu

Peak elution order can change dramatically when using
Bonus-RP. In this example, the elution order of the first
three peaks changes.

15

3

10
4

5

2

0
0

2

4

6

8

10

Time (min)

LCBR005

ZORBAX Bonus-RP
Hardware Description

Particle
Size (mm) Size (µm)

Bonus-RP
USP L60

Standard Columns (no special hardware required)

Analytical
Analytical
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar

4.6 x 250
4.6 x 150
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 75
4.6 x 50
4.6 x 100
4.6 x 75
4.6 x 50

5
5
3.5
3.5
3.5
3.5
3.5
1.8
1.8
1.8

880668-901
883668-901
884950-577
863668-901
864668-901
866668-901
835668-901
828668-901
830668-901
827668-901

Unless indicated, column pressure limit is 400 bar.

(Continued)

848

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

ZORBAX Bonus-RP
Hardware Description

Particle
Size (mm) Size (µm)

Bonus-RP
USP L60

3.0 x 250
3.0 x 150
3.0 x 150
3.0 x 100
3.0 x 100
3.0 x 50
2.1 x 150
2.1 x 50
2.1 x 150
2.1 x 100
2.1 x 50
2.1 x 100
2.1 x 50
1.0 x 150
1.0 x 50
1.0 x 30
1.0 x 17
4.6 x 12.5
2.1 x 12.5

5
5
3.5
3.5
1.8
1.8
5
5
3.5
3.5
3.5
1.8
1.8
3.5
3.5
3.5
5
5
5

880668-301
883668-301
863668-301
864668-301
828668-301
827668-301
883725-901
861971-901
863700-901
861768-901
861700-901
828768-901
827768-901
863608-901
865608-901
861608-901
5185-5922
820950-928
821125-928
820999-901

Standard Columns (no special hardware required)

Solvent Saver
Solvent Saver
Solvent Saver Plus
Solvent Saver Plus
Solvent Saver HT, 600 bar
Solvent Saver HT, 600 bar
Narrow Bore
Narrow Bore
Narrow Bore RR
Narrow Bore RR
Narrow Bore RR
Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
MicroBore RR
MicroBore RR
MicroBore RR
MicroBore Guard, 3/pk
Guard Cartridge, 4/pk
Guard Cartridge, 4/pk
Guard Hardware Kit

PrepHT Cartridge Columns (require endfittings kit 820400-901)

PrepHT Cartridge

21.2 x 250

7

878250-101

PrepHT Cartridge

21.2 x 150

7

878150-101

PrepHT Cartridge

21.2 x 150

5

868150-901

PrepHT Cartridge

21.2 x 100

5

868100-901

PrepHT Cartridge

21.2 x 50

5

868050-901

17 x 7.5

5

PrepHT endfittings, 2/pk
PrepHT Guard Cartridge, 2/pk
Guard Cartridge Hardware

820400-901
820212-928
820444-901

Unless indicated, column pressure limit is 400 bar.

LC AND LC/MS COLUMNS

849

Columns for Analytical HPLC

ZORBAX 80Å Extend-C18
O

O

C18
Si

Si

C18

Silica Support

Novel Bidentate C18-C18 Bonding for Extend C-18
Bonded Phase

• High efficiency and long life at high pH – up to pH 11.5
• Unique bidentate bonding and double endcapping provides high pH stability
• More efficiency and better peak shape than polymer-based columns
• Improve retention, resolution and peak shape of basic compounds
• High sensitivity for LC/MS separations of peptides
The Agilent ZORBAX Extend-C18 column uses a novel bidentate C18-C18 bonding technology to
make it possible to develop high-resolution separations at high pH with a silica-based column. At
high pH, non-charged basic compounds will not interact with the underlying silica. The result is
high efficiency separations with superior peak shape and improved resolution. High pH
separations are also the best choice for compounds that are more stable or more soluble in high
pH solutions. Some of the mobile phase buffer options for high pH include triethylamine,
pyrrolidine, glycine, borate and ammonium hydroxide. Ammonium hydroxide at pH 10.5 is an
excellent mobile phase modifier for the LC/MS of peptides and small molecules with improved
sensitivity compared with TFA containing mobile phase at low pH. The Extend-C18 column is
stable from pH 2-11.5 with good peak shape for all types of compounds. Extend-C18 columns
also provide an additional selectivity choice at low pH.

Column Specifications
Bonded Phase
ZORBAX Extend-C18

Pore
Size
80Å

Surface
Area
180 m2/g

Temp.
Limits*
60°C

pH Range**
2.0-11.5

Endcapped
Double

Carbon
Load
12.5%

Specifications represent typical values only.
*Temperature limits are 60°C up to pH 8, 40°C from pH 8-11.5.
**Above pH 6 highest column stability for all silica based columns is obtained by reducing the operating temperature to 40°C or
below and using lower buffer concentrations (0.01-0.02 M) or organic buffers.

850

LC AND LC/MS

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www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

Basic Antihistamines on Extend-C18
at High pH
Column:

Mobile Phase:

Flow Rate:
Temperature:
Detector:
Sample:

pH 7

ZORBAX Extend-C18
773450-902
4.6 x 150 mm, 5 µm

7

4

1.
2.
3.
4.
5.
6.
7.

2, 3
1

pH 7:
30% 20 mM Na2HPO4 70% MeOH
pH 11:
30% 20 mM TEA 70% MeOH
1.0 mL/min
Ambient
254 nm
Antihistamines

5
6

0

Maleate
Scopolamine
Pseudoephedrine
Doxylamine
Chlorpheniramine
Triprolidine
Diphenhydramine

5
Time (min)
7

pH 11

4
3
5

1

Pseudoephedrine and scopolamine are difficult to retain at low
and mid pH. Pseudoephedrine is often analyzed by ion exchange
methods. The Extend-C18 column retains these compounds in
a noncharged form at high pH and improves resolution.

2

0

6

5

10
Time (min)

LC80001

Long Life at High pH with Extend-C18
ZORBAX Extend-C18
773450-902
4.6 x 150 mm, 5 µm

Mobile Phase:

20% Methanol
80% 0.1 M Carbonate Buffer, pH 10.0
1.0 mL/min
Ambient

Flow Rate:
Temperature:

At high pH, columns will fail due to silica dissolution.
The example here shows extended lifetime of ZORBAX
Extend-C18 at high pH in comparison to competitor W.
This was measured by the amount of dissolved silica.

30

ZORBAX Extend-C18
Competitor W
Amount of Dissolved Silica (mg)

Column:

20

10

0
0

3

5

9

12

15

Volume Eluent (L)
LC80002

LC AND LC/MS COLUMNS

851

Columns for Analytical HPLC

Extend-C18 Provides Good Peak Shape at
Low pH
Column:

ZORBAX Extend-C18
773450-902
4.6 x 150 mm, 5 µm

Mobile Phase:

80% 25 mM NaH2PO4, pH 3.0
20% Methanol
1.0 mL/min
35°C
254 nm
Basic Compounds

Flow Rate:
Temperature:
Detector:
Sample:

1

1.
2.
3.
4.

3

4

2

These basic compounds are separated on the Extend-C18
at low pH with excellent peak shape. The Extend-C18
column can be used at high and low pH.

0

1

2

3

Theobromine
Dimethylxanthine
Theophylline
Caffeine

4

5

6

Time (min)

7
LC80003

ZORBAX 80Å Extend-C18
Hardware Description

Extend-C18
Size (mm) Particle Size (µm) USP L1

Standard Columns (no special hardware required)

Analytical
Analytical
Analytical
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Solvent Saver
Solvent Saver
Solvent Saver Plus
Solvent Saver Plus
Solvent Saver Plus

4.6 x 250
4.6 x 150
4.6 x 50
4.6 x 150
4.6 x 100
4.6 x 75
4.6 x 50
4.6 x 100
4.6 x 50
4.6 x 30
4.6 x 20
3.0 x 250
3.0 x 150
3.0 x 150
3.0 x 100
3.0 x 50

5
5
5
3.5
3.5
3.5
3.5
1.8
1.8
1.8
1.8
5
5
3.5
3.5
3.5

770450-902
773450-902
746450-902
763953-902
764953-902
766953-902
735953-902
728975-902
727975-902
724975-902
726975-902
770450-302
773450-302
763954-302
764953-302
735954-302

Unless indicated, column pressure limit is 400 bar.

(Continued)

852

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

ZORBAX 80Å Extend-C18
Hardware Description

Extend-C18
Size (mm) Particle Size (µm) USP L1

Standard Columns (no special hardware required)

Solvent Saver RRHD, 1200 bar
Solvent Saver RRHD, 1200 bar
Solvent Saver HT, 600 bar
Solvent Saver HT, 600 bar
Solvent Saver HT, 600 bar
Solvent Saver HT, 600 bar
Narrow Bore
Narrow Bore
Narrow Bore RR
Narrow Bore RR
Narrow Bore RRHD, 1200 bar
Narrow Bore RRHD, 1200 bar
Narrow Bore RRHD, 1200 bar
Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
Narrow Bore RRHT, 600 bar
MicroBore RR
MicroBore RR
MicroBore RR
MicroBore Guard, 3/pk
Guard Cartridge, 4/pk
Guard Cartridge, 4/pk
Guard Hardware Kit

3.0 x 100
3.0 x 50
3.0 x 100
3.0 x 50
3.0 x 30
3.0 x 20
2.1 x 150
2.1 x 50
2.1 x 100
2.1 x 50
2.1 x 150
2.1 x 100
2.1 x 50
2.1 x 100
2.1 x 50
2.1 x 30
2.1 x 20
1.0 x 150
1.0 x 50
1.0 x 30
1.0 x 17
4.6 x 12.5
2.1 x 12.5

1.8
1.8
1.8
1.8
1.8
1.8
5
5
3.5
3.5
1.8
1.8
1.8
1.8
1.8
1.8
1.8
3.5
3.5
3.5
5
5
5

758700-302
757700-302
728975-302
727975-302
724975-302
726975-302
773700-902
760450-902
761753-902
735700-902
759700-902
758700-902
757700-902
728700-902
727700-902
724700-902
726700-902
763600-902
765600-902
761600-902
5185-5923
820950-930
821125-930
820999-901

PrepHT Cartridge Columns (require endfittings kit 820400-901)

PrepHT Cartridge

21.2 x 150

5

770150-902

PrepHT

21.2 x 100

5

770100-902

PrepHT

21.2 x 50

5

770050-902

17 x 7.5

5

PrepHT endfittings, 2/pk
PrepHT Guard Cartridge, 2/pk
Guard Cartridge Hardware

820400-901
820212-930
820444-901

Unless indicated, column pressure limit is 400 bar.

LC AND LC/MS COLUMNS

853

Columns for Analytical HPLC

ZORBAX Rx
• Recommended for alternate selectivity at low pH relative to Eclipse XDB-C18 and

StableBond SB-C18; for higher temperature applications, StableBond is recommended
• Higher carbon load than SB-C18 columns (12% vs. 10%).
• High stability and good peak shape for low pH applications (up to pH 8)
• Manufactured using dimethyloctadecylsilane and non-endcapped
• Same product as SB-C8

Column Specifications
Bonded Phase
ZORBAX Rx-C18
ZORBAX Rx-C8

Pore
Size
80Å
80Å

Surface
Area
180 m2/g
180 m2/g

Temp.
Limits
60°C
80°C

pH
Range*
2.0-8.0
1.0-8.0

Endcapped
No
No

Carbon
Load
12%
5.5%

Specifications represent typical values only.
*At pH 6-9 highest column stability for all silica based columns is obtained by operating at temperatures <40°C and using lower
buffer concentrations in the range of 0.01-0.02 M.

Analysis of Diazepam on Rx-C18
Column:

ZORBAX Rx-C18
880967-302
3.0 x 250 mm, 5 µm

Mobile Phase:
Flow Rate:

35% H2O:65% MeOH
0.5 mL/min

An Rx-C18 column is used for this USP analysis of diazepam and the internal
standard ethylparaben. The Solvent Saver 3.0 mm ID Rx-C18 column reduces
solvent usage by 60% over what would be used if the analysis was done
on a 4.6 x 250 mm column.

854

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

1
2

1. Ethylparaben
2. Diazepam

0.0

2.5
Time (min)

5.0

7.5
LCRX001

l 01357 522 961

Columns for Analytical HPLC

ZORBAX Rx
Hardware Description
Semi-Preparative
Analytical
Analytical
Rapid Resolution
Rapid Resolution
Rapid Resolution
Solvent Saver
Solvent Saver
Solvent Saver Plus
Solvent Saver Plus
Narrow Bore
Narrow Bore RR
Guard Cartridge, 2/pk
Guard Cartridge, 4/pk
Guard Cartridge, 4/pk
Guard Hardware Kit
Guard Hardware Kit

Size (mm)
9.4 x 250
4.6 x 250
4.6 x 150
4.6 x 150
4.6 x 100
4.6 x 75
3.0 x 250
3.0 x 150
3.0 x 150
3.0 x 100
2.1 x 150
2.1 x 100
9.4 x 15
4.6 x 12.5
2.1 x 12.5
9.4 x 15

Particle
Size (µm)
5
5
5
3.5
3.5
3.5
5
5
3.5
3.5
5
3.5
7
5
5

Rx-C18
USP L1
880967-202
880967-902
883967-902
863967-902
861967-902
866967-902
880967-302
883967-302
863967-302
861967-302
883700-902
861767-902
820675-115
820950-914
821125-915
840140-901
820999-901

Rx-C8
USP L7*
880967-201
880967-901
883967-901

820675-115
820950-913
821125-915
840140-901
820999-901

PrepHT Cartridge Columns (require endfittings kit 820400-901)

PrepHT Cartridge

21.2 x 250

7

877967-102 877250-106

PrepHT Cartridge

21.2 x 150

7

877150-106

PrepHT Cartridge

21.2 x 150

5

870150-906

PrepHT Cartridge

21.2 x 100

5

870100-906

PrepHT Cartridge

21.2 x 50

PrepHT Guard Cartridge, 2/pk

5

870050-906

5

820212-914 820212-915

Guard Cartridge Hardware

820444-901 820444-901

PrepHT endfittings, 2/pk

820400-901 820400-901

*Rx-C8 is the same product as SB-C8. For other sizes and configurations, see the ZORBAX StableBond section.
Turn to pages 841–848.

LC AND LC/MS COLUMNS

855

Columns for Analytical HPLC

Pursuit HPLC Columns
Beginning in drug discovery and drug metabolism, Pursuit columns are ideal for analyzing lead
compounds and biological samples. The column's performance is due to the unique combination
of advanced bonding chemistry and ultra-high purity silica. These factors combine to provide
rapid separations with excellent first time resolution and symmetrical peaks for polar compounds,
whether at pH 1.5 or 10. Additionally, the need for ion pairing agents such as TFA is often
eliminated, thus maximizing the performance of single and parallel multi-channel LC/MS systems.
Culminating in QC, Pursuit is ideal for implementing dependable trouble-free analysis of raw
materials and approved drugs. Rigorous control and validation of each step in the manufacturing
process ensures column reproducibility. With Pursuit your laboratory can spend its energy on
producing results.
Special selectivities such as Pursuit PFP (for very polar compounds) and Pursuit PAH
(environmental) give you the extra selectivities you need for your most challenging applications.

Pursuit
For LC/MS and high throughput applications. Built on the larger 200Å pore size silica, high ligand
density delivers up to 40% faster separations without sacrificing resolution. This is accomplished
by optimizing mass transfer with the larger pore size.

Pursuit XRs
For performance in analytical R&D, QC and preparative applications. Combining high ligand
density with a 100Å pore size, high surface area silica, Pursuit XRs columns are designed to
increase productivity, as they offer maximum loadability, excellent stability and easy scalability
while maintaining superior resolution.

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

856

LC AND LC/MS

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l 01357 522 961

Columns for Analytical HPLC

Pursuit XRsUltra 2.8
For the ultimate in speed and good resolution on any instrument, we designed the Pursuit
XRsUltra 2.8 around an optimized 2.8 µm particle and an advanced packing procedure.
Now you can decrease your run time while maintaining resolution. Lower backpressure
allows high flow rates to be used, and the 2.8 µm particles of ultra-pure silica delivers
10-15% higher efficiency than 3 µm columns.

Pursuit UPS2.4
For maximum efficiency, particularly in high viscosity solvent separations. With an optimized
2.4 µm particle, Pursuit UPS columns offer approximately 50% lower backpressure compared
to sub-2 µm columns, delivering higher speed and resolution without the need for ultra-high
pressure equipment.

Pursuit UPS1.9
Pursuit UPS1.9 columns deliver sub-2 µm efficiencies when sensitivity, resolution, and throughput
are critical. These columns excel under the high pressures and fast gradients demanded by
today's pharmaceutical industry, up to a pressure limit of 1000 bar.

Column Specifications
Bonded Phase
Pursuit C18
Pursuit C8
Pursuit Diphenyl
Pursuit PFP
Pursuit PAH
Pursuit XRs C18
Pursuit XRs C8
Pursuit XRs Diphenyl
Pursuit XRs Si
Pursuit XRsUltra 2.8 C18
Pursuit XRsUltra 2.8 C8
Pursuit XRsUltra 2.8
Diphenyl
Pursuit UPS2.4 C18

Pore Size
200Å
200Å
200Å
200Å
200Å
100Å
100Å
100Å
100Å
100Å
100Å
100Å

Surface Area
200 m2/g
200 m2/g
200 m2/g
200 m2/g
200 m2/g
440 m2/g
440 m2/g
440 m2/g
440 m2/g
440 m2/g
440 m2/g
440 m2/g

pH Range
1.5-10
1.5-10
1.5-8.0
1.5-10
1.5-10
1.5-10
1.5-10
1.5-8.0
1.5-10
1.5-10
1.5-10
1.5-8.0

Endcapped
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes

100Å

350 m2/g

1.5-10

100Å

350 m2/g

1.5-10

Pursuit UPS1.9 C18

Carbon
Load
12.9%
7.4%
7.3%
6.3%

23.2%
15%
14.6%

Pore Volume
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g
1.1 mL/g

Yes

21%

0.9 mL/g

2.5 µmol/m2

Yes

21%

0.9 mL/g

3.0 µmol/m2

22%
15%
14.6%

Ligand Coverage
3.5 µmol/m2
3.8 µmol/m2
2.8 µmol/m2
3.4 µmol/m2
2.9 µmol/m2
3.7 µmol/m2
2.6 µmol/m2
3.2 µmol/m2
3.7 µmol/m2
2.6 µmol/m2

Specifications represent typical values only.

LC AND LC/MS COLUMNS

857

Columns for Analytical HPLC

Tricyclic antidepressants and benzodiazepines
Column:

Pursuit XRs C18
A6000150X046
4.6 x 150 mm, 5 µm

Mobile Phase:

A: Water+0.1% HCOOH
B: MeCN+0.1% HCOOH
30-40% B in 15 min, hold at 40% B for 15 min
1.0 mL/min
Ambient
UV, 254 nm

24
1

2
3

mAU

Gradient:
Flow Rate:
Temperature:
Detector:

1.
2.
3.
4.
5.
6.
7.

5
4

67

8
9

8.
9.
10.
11.
12.
13.
14.

7-Aminoclonazepam
7-Aminoflunitrazepam
Nordoxepin
Doxepin
Desipramine
Imipramine
Nortriptyline

Amitriptyline
Trimipramine
Clomipramine
Nordiazepam
Clonazepam
Flunitrazepam
Diazepam

10
11

14

13

12

0

min

0

24
VLC0001

Mechanical stability of Pursuit XRs
Pursuit XRs C18
A6000050X020
2.0 x 50 mm, 5 µm

Sample:
Mobile Phase:

DMSO mix
A: MeOH:water, 10:90 + 0.1% HCOOH
B: MeOH:water, 90:10 + 0.1% HCOOH
0-100% B in 3 min, back to 0% B
in 0.5 min, hold at 0% B for 3.5 min
0.4 mL/min
Ambient
UV, 254 nm

Gradient:
Flow Rate:
Temperature:
Detector:

1. 4-Methoxybenzenesulfonamide
2. Methyl 3-aminothiophene-2-carboxylate
3. Trimipramine

Injection 1

1

2

3

400
mAU

Column:

0

0

min

Injection 5000

6

1

2
3

mAU

400

0

0

858

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

min

6

VLC0002

Columns for Analytical HPLC

Antifungals
Pursuit XRsUltra 2.8 Diphenyl
A7521050X020
2 x 50 mm, 2.8 µm

Mobile Phase:

Water+0.1% HCOOH:MeCN+0.1%
HCOOH, 80:20
0.4 mL/min
Ambient
UV, 254 nm

Flow Rate:
Temperature:
Detector:

1.
2.
3.
4.

2

240,000
1

4
3

µV

Column:

4-Aminobenzoic acid
Sorbic acid
Benzoic acid
Salicylic acid

0

0

min

6
VLC0003

Liquid chromatography phase test mixture
(LPTM) on Pursuit C8
Column:

Mobile Phase:
Flow Rate:
Detector:

2

A: 0.05% HCOOH in water
B: 0.05% HCOOH in MeCN
0.6 mL/min
UV, 220 nm

3

Aspartame
Cortisone
Reserpine
Dioctyl phthalate

1

2

min

5

Adrenocorticosteroids on Pursuit PFP and C18

300

1 2

3
4

mAU

Pursuit PFP

5

7

6

10

9

8

VLC0004

1.
2.
3.
4.
5.
6.
7.
8.
9.
10.

MeCN:water, 22.5:77.5
1.5 mL/min
Ambient
UV, 240 nm

Triamcinolone
Prednisolone
Cortisone
Methylprednisolone
Corticosterone
Beclomethasone
Prednisolone acetate
Triamcinolone acetonide
Cortisone acetate
Fluocinolone acetonide

0

0

Pursuit C18

300

min

1

mAU

Mobile Phase:
Flow Rate:
Temperature:
Detector:

1.
2.
3.
4.

4

Pursuit C8
A3031050X020
2.0 x 50 mm, 3 µm

2

25

3
4

10
5

6 7

8

9

0

0

min

25
VLC0005

LC AND LC/MS COLUMNS

859

Columns for Analytical HPLC

Pursuit HPLC Columns
Size (mm)
50 x 250
21.2 x 250
21.2 x 150
21.2 x 250
21.2 x 150
21.2 x 100
10 x 250
10 x 150
10 x 250
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 50
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 50
4.6 x 30
4.0 x 250
4.0 x 125
3.9 x 300
3.9 x 300
3.9 x 150
3.0 x 250
3.0 x 150
3.0 x 100
3.0 x 250
3.0 x 150
3.0 x 100
3.0 x 50
2.0 x 250
2.0 x 150

Particle Size (µm)
10
10
10
5
5
5
10
5
5
10
10
10
5
5
5
5
3
3
3
3
3
5
5
10
5
5
5
5
5
3
3
3
3
5
5

Pursuit C18
USP L1
A3002250X500
A3002250X212
A3002150X212
A3000250X212
A3000150X212

Pursuit C8
USP L7
Pursuit Diphenyl Pursuit PFP
A3032250X500
A3032250X212

A3002250X100
A3000150X100
A3000250X100
A3002250X046
A3002150X046

A3032250X100

Pursuit PAH
USP L1

A3050250X212
A3050150X212
A3040100X212

A3000250X046
A3000150X046
A3000100X046
A3000050X046
A3001250X046
A3001150X046
A3001100X046
A3001050X046
A3001030X046
A3000250X040
A3000125X040
A3002300X039
A3000300X039
A3000150X039
A3000250X030
A3000150X030
A3000100X030
A3001250X030
A3001150X030
A3001100X030
A3001050X030
A3000250X020
A3000150X020

A3050150X100
A3050250X100

A3030250X100
A3032250X046
A3032150X046
A3030250X046
A3030150X046
A3030100X046
A3030150X046
A3031250X046
A3031150X046
A3031100X046

A3040250X046
A3040150X046
A3040100X046
A3040050X046
A3041250X046
A3041150X046
A3041100X046
A3041050X046

A3040250X030
A3040150X030

A3041150X030
A3041100X030
A3041050X030

A3050250X046 A7000250X046
A3050150X046 A7000150X046
A3050100X046
A3050050X046
A3051250X046
A3051150X046
A3051100X046 A7001100X046
A3051050X046

A3050150X030
A3050100X030
A3051150X030
A3051100X030 A7001100X030
A3051050X030

A3030150X020 A3040150X020
(Continued)

860

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

Pursuit HPLC Columns
Size (mm)
2.0 x 100
2.0 x 50
2.0 x 30
2.0 x 20
2.0 x 250
2.0 x 200
2.0 x 150
2.0 x 100
2.0 x 50
2.0 x 30
2.0 x 20

Particle Size (µm)
5
5
5
5
3
3
3
3
3
3
3

Pursuit C18
USP L1
A3000100X020
A3000050X020
A3000030X020
A3000020X020
A3001250X020

Pursuit C8
USP L7
Pursuit Diphenyl
A3030100X020 A3040100X020
A3030050X020 A3040050X020
A3040030X020

A3001150X020
A3001100X020
A3001050X020
A3001030X020
A3001020X020

A3031150X020
A3031100X020
A3031050X020
A3031030X020

A3041250X020
A3041200X020
A3041150X020
A3041100X020
A3041050X020
A3041030X020
A3041020X020

Pursuit PAH
Pursuit PFP
USP L1
A3050100X020
A3050050X020
A3050030X020
A3050020X020

A3051150X020
A3051100X020 A7001100X020
A3051050X020
A3051030X020
A3051020X020

Pursuit ChromSep Complete Cartridge Systems
Hardware

Size (mm)
4.6 x 250
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 150
4.6 x 100
4.6 x 50
3.0 x 250
3.0 x 150
3.0 x 100
3.0 x 150
3.0 x 100
2.0 x 250
2.0 x 150
2.0 x 100
2.0 x 150
2.0 x 100
2.0 x 50

Particle Size (µm)
5
3
5
5
3
3
3
5
5
5
3
3
5
5
5
3
3
3

Pursuit C18
USP L1
A3000250C046
A3000150C046
A3000100C046
A3001150C046
A3001100C046
A3001050C046
A3000250C030
A3000150C030
A3000100C030
A3001150C030
A3001100C030
A3000250C020
A3000150C020
A3000100C020
A3001150C020
A3001100C020
A3001050C020

Pursuit C8
USP L7
A3030250C046
A3031250C046
A3030150C046
A3030100C046
A3031150C046
A3031100C046

Pursuit PAH
USP L1
A7000250C046
A7000150C046
A7001150C046
A7001100C046

A7000100C030

A3030150C020

LC AND LC/MS COLUMNS

861

Columns for Analytical HPLC

Pursuit ChromSep Replacement Cartridges
Hardware

Size (mm)
4.6 x 250

Particle Size (µm)
5

4.6 x 150

5

Unit

Pursuit C18
USP L1

Pursuit C8
USP L7

3/pk
3/pk
4.6 x 150

3

4.6 x 100

3

4.6 x 50

3

3/pk

A3000150R046 A3030150R046
A3000150T046 A3030150T046
A3031150R046
A3031150T046

3/pk
3/pk
3.0 x 150

5

3.0 x 100

5

3/pk
3/pk
3.0 x 150

3
3/pk

3.0 x 100

3
3/pk

2.0 x 50

A3001050R046
A3001050T046
A3000150R030
A3000150T030
A3000100R030
A3000100T030
A3001150R030
A3001150T030
A3001100R030
A3001100T030

3

Pursuit PAH
USP L1
A7000250R046
A7000250T046
A7000150R046
A7000150T046
A7001150R046
A7001150T046
A7001100R046
A7001100T046

A7000100R030
A7000100T030

A7001100R030
A7001100T030
A3031050R020
A3031050T020

3/pk

Pursuit XRs HPLC Columns
Size
Particle
(mm)
Size (µm)
50.0 x 250
10
30.0 x 250
5
30.0 x 150
5
30.0 x 100
5
30.0 x 50
5
21.2 x 250
10
21.2 x 250
5
21.2 x 150
5
21.2 x 100
5
21.2 x 50
5
21.2 x 30
5

Pursuit
XRs C18
USP L1
A6002250X500
A6000250X300
A6000150X300
A6000100X300
A6000050X300
A6002250X212
A6000250X212
A6000150X212
A6000100X212
A6000050X212
A6000030X212

Pursuit
XRs C8
USP L7

Pursuit
Pursuit
XRs Si
XRs Diphenyl USP L3
A6002250X500 A6004250X500
A6004250X300
A6020150X300

A6012250X212

A6004250X212
A6020250X212
A6020100X212

(Continued)

862

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

Pursuit XRs HPLC Columns
Size
Particle
(mm)
Size (µm)
10.0 x 250
10
10.0 x 250
5
10.0 x 150
5
10.0 x 50
5
10.0 x 150
3
4.6 x 250
10
4.6 x 50
10
4.6 x 250
5
4.6 x 150
5
4.6 x 100
5
4.6 x 50
5
4.6 x 250
3
4.6 x 150
3
4.6 x 100
3
4.6 x 50
3
4.6 x 30
3
4.0 x 250
5
4.0 x 150
5
3.0 x 250
5
3.0 x 150
5
3.0 x 100
5
3.0 x 150
3
3.0 x 100
3
3.0 x 50
3
3.0 x 30
3
2.1 x 100
5
2.0 x 250
5
2.0 x 150
5
2.0 x 100
5
2.0 x 50
5
2.0 x 30
5
2.0 x 250
3
2.0 x 150
3
2.0 x 100
3
2.0 x 50
3
2.0 x 30
3
2.0 x 20
3
1.0 x 150
3
1.0 x 100
3

Pursuit
XRs C18
USP L1
A6002250X100
A6000250X100
A6000150X100
A6000050X100

Pursuit
XRs C8
USP L7

Pursuit
XRs Diphenyl

Pursuit
XRs Si
USP L3
A6004250X100

A6020250X100

A6021150X100
A6002250X046
A6002050X046S
A6000250X046
A6000150X046
A6000100X046
A6000050X046
A6001250X046
A6001150X046
A6001100X046
A6001050X046
A6001030X046
A6000250X040
A6000150X040
A6000250X030
A6000150X030
A6000100X030
A6001150X030
A6001100X030
A6001050X030
A6001030X030

A6004250X046
A6010250X046 A6020250X046
A6010150X046 A6020150X046
A6010100X046 A6020100X046 A6006100X046
A6020050X046 A6006050X046
A6021250X046
A6010150X046 A6021150X046
A6011100X046 A6021100X046 A6005100X046
A6011050X046 A6021050X046 A6005050X046
A6021030X046
A6010250X040
A6010150X040

A6021150X030
A6021100X030
A6021050X030
A6006100X021

A6000250X020
A6000150X020
A6000100X020
A6000050X020
A6000030X020
A6001250X020
A6001150X020
A6001100X020
A6001050X020
A6001020X020
A6001150X010
A6001100X010

A6020250X020
A6010150X020 A6020150X020
A6010100X020
A6010050X020 A6020050X020
A6021250X020
A6011150X020 A6021150X020
A6011100X020 A6021100X020
A6011050X020 A6021050X020 A6005050X020
A6021030X020

A6021100X010

LC AND LC/MS COLUMNS

863

Columns for Analytical HPLC

Pursuit XRsUltra 2.8 HPLC Columns
Size (mm)
3.0 x 150
3.0 x 100
2.0 x 150
2.0 x 100
2.0 x 50
2.0 x 30

Particle Size
(µm)
2.8
2.8
2.8
2.8
2.8
2.8

Pursuit
XRsUltra 2.8 C18
A7501150X030
A7501100X030
A7501150X020
A7501100X020
A7501050X020
A7501030X020

Pursuit
XRsUltra 2.8 C8
A7511150X030

Pursuit XRsUltra 2.8
Diphenyl

A7511100X020
A7511050X020
A7511030X020

A7521100X020
A7521050X020
A7521030X020

Pursuit UPS2.4 HPLC Columns
Size (mm)
3.0 x 100
3.0 x 50
2.0 x 100
2.0 x 50
2.0 x 30

Particle Size (µm)
2.4
2.4
2.4
2.4
2.4

Pursuit UPS2.4
A8100100X030H
A8100050X030H
A8100100X020H
A8100050X020H
A8100030X020H

Pursuit UPS1.9 HPLC Columns
Size (mm)
3.0 x 100
3.0 x 50
2.0 x 100
2.0 x 50
2.0 x 30

Particle Size (µm)
1.9
1.9
1.9
1.9
1.9

Pursuit
UPS1.9 C18
A8000100X030H
A8000050X030H
A8000100X020H
A8000050X020H
A8000030X020H

Tips & Tools
Agilent offers a variety of e-Seminars and on-site training
to help you learn how to be a more effective chromatographer.
For more information, visit www.agilent.com/chem/education

864

LC AND LC/MS

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www.crawfordscientific.com

l 01357 522 961

Pursuit UPS1.9
Diphenyl
A8020100X030H
A8020050X030H
A8020100X020H
A8020050X020H
A8020030X020H

Columns for Analytical HPLC

Polaris HPLC Columns
In areas like drug discovery where target compounds are increasingly polar, it is critical to have
a reverse phase column that performs well under aqueous conditions. Retention is critical, but
cannot come with troublesome secondary interactions. Likewise, phase collapse and shifting
retention times need to be avoided. The answer is our Polaris line of polar-modified columns.
From the collapse-resistant pore structure of our base silica, to the "wettability" engineered
into the bonded phases, Polaris columns have been designed for high aqueous conditions.
The combination of high phase density bonding, ultra pure silica, and silanol shielding leads
to excellent peak shape among polar-modified columns.
As a family, Polaris offers a variety of polar modifications in both C18 and C8 chemistries.

Polaris C18-A
Polaris C18-A is the best starting place for separations where the benefits of polar-modified
columns are desired. The polar modifications of C18-A help it avoid poor peak shape and
retention issues in low organic conditions.

Polaris C8-A
Polaris C8-A offers an alternative selectivity to standard C8 phases and has a lower
hydrophobicity than Polaris C18-A, making it ideal for polar samples, or faster overall
analysis times.

Polaris C18-Ether
Polaris C18-Ether offers an alternative selectivity to Polaris C18-A and standard C18 phases,
and typically delivers increased retention of polar compounds away from the void volume.

Polaris C8-Ether
Polaris C8-Ether offers an alternative selectivity to Polaris C8-A with particular utility for hydrogen
bonding compounds.

LC AND LC/MS COLUMNS

865

Columns for Analytical HPLC

Column Specifications
Bonded Phase Pore Size
Polaris C18-A
180Å
Polaris C8-A
180Å
Polaris C18-Ether
180Å
Polaris C8-Ether
180Å

Surface Carbon
Area
Load
200 m2/g 13.8%
200 m2/g 7.4%
200 m2/g 12.1%
200 m2/g 7.1%

Pore
Volume
1.1 cm3/g
1.1 cm3/g
1.1 cm3/g
1.1 cm3/g

Endcapped
Yes
Yes
Yes
Yes

Ligand
Coverage
3.9 µmol/m2
4.8 µmol/m2
3.3 µmol/m2
4.5 µmol/m2

Specifications represent typical values only.

Selectivity text mix for Polaris columns
MeCN:water 70:30
1.0 mL/min
Ambient
UV, 254 nm

Polaris C8-A

1.
2.
3.
4.

5+8
6

400
mAU

Mobile Phase:
Flow Rate:
Temperature:
Detector:

12 3

4

7

Uracil
Caffeine
Phenol
Toluene

5.
6.
7.
8.

Butylbenzene
o-Terphenyl
Amylbenzene
Triphenylene

0

0

min

14

Polaris C18-A
400

7

5

mAU

1

8

6

4

2 3

0

0

min

14

VLC0007

LC/MS performance test mix for Polaris C8-A
Polaris C8-A
A2011030X030
3.0 x 30 mm, 3 µm

Mobile Phase:

A: Water+0.05% HCOOH
B: MeCN+0.05% HCOOH
5-90% B in 3 min and hold for 4 min
0.6 mL/min
Ambient
UV, 220 nm

Gradient:
Flow Rate:
Temperature:
Detector:

866

LC AND LC/MS

1.
2.
3.
4.

3
4
2

400

Aspartame
Cortisone
Reserpine
Dioctyl phthalate

1

mAU

Column:

0

0

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

min

l 01357 522 961

8

VLC0008

Columns for Analytical HPLC

Polaris HPLC Columns
Size (mm)
50 x 250
30 x 100
21.2 x 250
21.2 x 250
21.2 x 150
21.2 x 100
21.2 x 50
10 x 250
10 x 50
4.6 x 250
4.6 x 250
4.6 x 200
4.6 x 150
4.6 x 100
4.6 x 50
4.6 x 30
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 75
4.6 x 50
4.6 x 30
4.0 x 250
4.0 x 150
4.0 x 125
3.0 x 250
3.0 x 150
3.0 x 100
3.0 x 50
3.0 x 250
3.0 x 200
3.0 x 150
3.0 x 100
3.0 x 50
3.0 x 30

Particle
Size (µm)
10
5
10
5
5
5
5
5
3
10
5
5
5
5
5
5
3
3
3
3
3
3
5
5
5
5
5
5
5
3
3
3
3
3
3

Polaris
C18-A
A2002250X500
A2000100X300
A2002250X212
A2000250X212
A2000150X212
A2000100X212

Polaris
C8-A

Polaris
C18-Ether

Polaris
C8-Ether

Polaris
NH2

A2010250X212

A2020250X212

A2030250X212

A2013250X212

Polaris
Si-A
A2004250X500
A2004250X212
A2003250X212
A2003150X046
A2003050X212

A2000250X100
A2002250X046
A2000250X046
A2000200X046
A2000150X046
A2000100X046
A2000050X046
A2000030X046
A2001250X046
A2001150X046
A2001100X046
A2001075X046
A2001050X046
A2001030X046
A2000250X040
A2000150X040
A2000250X030
A2000150X030
A2000100X030
A2000050X030
A2001250X030
A2001200X030
A2001150X030
A2001100X030
A2001050X030
A2001030X030

A2020250X100
A2021050X100

A2030250X100

A2013250X100

A2010250X046

A2020250X046

A2030250X046

A2013250X046

A2010150X046
A2010100X046

A2020150X046

A2030150X046

A2013150X046
A2013100X046
A2013050X046

A2003150X046
A2003100X046
A2003050X046

A2021250X046

A2031250X046

A2014250X046
A2014150X046
A2014100X046

A2005250X046
A2005150X046
A2005100X046

A2021050X046

A2031050X046

A2014050X046

A2005050X046

A2013250X040
A2013150X040
A2013125X040
A2013250X030
A2013150X030
A2013100X030
A2014250X030

A2003250X040
A2003150X040
A2003125X040
A2005250X046
A2003150X030
A2003100X030
A2003050X030
A2003250X030

A2014150X030
A2014100X030
A2014050X030

A2005150X030
A2005100X030
A2005050X030

A2003250X046

A2020050X046

A2011150X046
A2011100X046
A2011075X046

A2020150X030

A2021150X030
A2021050X030

A2031050X030

A2011030X030
(Continued)

LC AND LC/MS COLUMNS

867

Columns for Analytical HPLC

Polaris HPLC Columns
Size (mm)
2.0 x 250
2.0 x 150
2.0 x 100
2.0 x 50
2.0 x 30
2.0 x 20
2.0 x 250
2.0 x 150
2.0 x 100
2.0 x 75
2.0 x 50
2.0 x 30
2.0 x 20

Particle
Size (µm)
5
5
5
5
5
5
3
3
3
3
3
3
3

Polaris
C18-A
A2000250X020
A2000150X020
A2000100X020
A2000050X020
A2000030X020
A2000020X020
A2001250X020
A2001150X020
A2001100X020

Polaris
C8-A
A2010150X020

Polaris
C18-Ether
A2020250X020
A2020150X020

Polaris
C8-Ether
A2030250X020
A2030150X020

A2010050X020

A2020050X020

A2030050X020

A2011250X020
A2011150X020

A2021250X020
A2021150X020
A2021100X020
A2021075X020
A2021050X020
A2021050X020

A2031250X020
A2031150X020
A2031100X020

A2001050X020
A2001030X020
A2001020X020

A2011050X020

A2031050X020

Tips & Tools
To learn more about Agilent's complete portfolio of services,
please visit www.agilent.com/chem/services

868

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Polaris
NH2
A2013250X020
A2013150X020
A2013100X020
A2013050X020
A2013030X020
A2013020X020
A2014250X020
A2014150X020
A2014100X020

Polaris
Si-A
A2003250X020
A2003150X020
A2003100X020
A2003050X020
A2003030X020
A2003020X020
A2005250X020
A2005150X020
A2005100X020

A2014050X020
A2014030X020
A2014020X020

A2005050X020
A2005030X020
A2005020X020

Columns for Analytical HPLC

Polaris ChromSep Complete Cartridge Systems
Hardware

Size (mm)
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 250
4.6 x 150
3.0 x 250
3.0 x 100
2.0 x 100
2.0 x 150
2.0 x 100
2.0 x 50

Particle Size (µm)
5
5
5
3
3
5
5
5
3
3
3

Polaris
C18-A
A2000250C046
A2000150C046
A2000100C046
A2001250C046
A2001150C046
A2000250C030
A2000100C030
A2000100C020
A2001150C020
A2001100C020
A2001050C020

Polaris ChromSep Replacement Cartridges
Hardware

Size (mm)
4.6 x 250

Particle Size (µm)
5

Unit
3/pk

4.6 x 150

5
3/pk

4.6 x 100

5

4.6 x 150

3

4.6 x 100

3

3/pk
3/pk
3/pk
3.0 x 150

5

3.0 x 100

5

3.0 x 100

3

2.0 x 150

3

2.0 x 50

3

3/pk
3/pk
3/pk
3/pk
3/pk

Polaris
C18-A
A2000250R046
A2000250T046
A2000150R046
A2000150T046
A2000100R046
A2000100T046
A2001150R046
A2001150T046
A2001100R046
A2001100T046
A2000150R030
A2000150T030
A2000100R030
A2000100T030
A2001100R030
A2001100T030
A2001150R020
A2001150T020
A2001050R020
A2001050T020

LC AND LC/MS COLUMNS

869

Columns for Analytical HPLC

ZORBAX Original Reversed-Phase Columns
Agilent Original ZORBAX columns are made with Type A silica and are useful for many applications of acidic or neutral compounds. These columns
have a higher activity level and are therefore useful for separating isomers (e.g. cis-trans, geometric) or other compounds where silanol activity
enhances selectivity. These columns are used in many established methods.

ZORBAX Original Reversed Phase Columns
Hardware Description

Size (mm)

Particle ODS (C18)
Size (µm) USP L1

C8
USP L7

Phenyl
USP L11

CN
USP L10

TMS
USP L13

Standard Columns (no special hardware required)

Semi-Preparative
Analytical (Endcapped)
Analytical (Non-endcapped)
Analytical
Solvent Saver
Solvent Saver

9.4 x 250
4.6 x 250
4.6 x 250
4.6 x 150
3.0 x 250
3.0 x 150

5
5
5
5
5
5

880952-202 880952-206
880952-702 880952-706 880952-712 884950-507 880952-710
884950-543
883952-702 883952-706 883952-712 884950-526 883952-710
880952-302
883952-302

9.4 x 15
4.6 x 12.5

7
5

820675-115
820950-902
840140-901
820999-901

7

877952-102 877952-106

877952-105

820400-901 820400-901

820400-901

Guard Columns (hardware required)

Guard Cartridge, 2/pk
Guard Cartridge, 4/pk
Guard Hardware Kit
Guard Hardware Kit

820675-115
820950-906
840140-901
820999-901

820675-115
820950-912
840140-901
820999-901

820675-124
820950-905 820950-924
840140-901 840140-901
820999-901 820999-901

PrepHT Cartridge Columns (require endfittings kit 820400-901)

PrepHT Cartridge

21.2 x 250

PrepHT endfittings, 2/pk

870

LC AND LC/MS

www.agilent.com/chem/lccolumns
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l 01357 522 961

Columns for Analytical HPLC

Agilent TC-C18(2) and HC-C18(2)
TC-C18(2)
Agilent TC-C18(2) is the ideal choice for complex natural product extract samples, traditional
medicines and environmental samples or any sample where you need to analyze mixtures
of polar and non-polar compounds, including strong basic compounds.

• Lower carbon load – 12%
• Ideal for polar compounds and gradient separations that start at low % organic or cover
a wide organic range

• Good choice for samples dissolved in water, or mostly water
• Use with most common mobile phases, including formic acid, acetic acid, trifluoroacetic acid
(TFA) and phosphate buffers with acetonitrile and methanol as the organic modifiers

• Excellent performance from pH 2-8
HC-C18(2)

Agilent HC-C18(2) is a more retentive C18 with a higher carbon load. An excellent value
alternative to other high carbon load columns, it also provides superior peak shape
for basic compounds.

• Higher carbon load – 17% – provides greater retention for moderately polar
and non-polar compounds

• Ideal for non-polar compounds and separations that start at mid-level % organic
(at least greater than 10% organic)

• Good choice for industrial samples or samples dissolved in organic/mostly organic solvents
• Stable over a very wide pH range (2-9) for maximum flexibility

LC AND LC/MS COLUMNS

871

Columns for Analytical HPLC

Column Specifications
Bonded
Phase
TC-C18 (2)
HC-C18 (2)

Pore
Size
170Å
170Å

Temp.
Surface Area Limits
60°C
290 m2/g
60°C
290 m2/g

Carbon
pH Range* Endcapped Load
2.0-8.0
Yes
12%
2.0-9.0
Yes
17%

Specifications represent typical values only.

Agilent HC-C18(2) and TC-C18(2)
Description
Agilent HC-C18(2)
Agilent HC-C18(2)
Agilent TC-C18(2)
Agilent TC-C18(2)
Agilent HC-C18(2) guards, 2/pk
Agilent TC-C18(2) guards, 2/pk
Guard Hardware Kit

Size (mm)
4.6 x 250
4.6 x 150
4.6 x 250
4.6 x 150
4.6 x 12.5
4.6 x 12.5

Particle Size (µm)
5
5
5
5
5
5

Tips & Tools
Don't forget, we have special offers throughout the year.
To learn more, visit www.agilent.com/chem/specialoffers

872

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Part No.
588905-902
588915-902
588925-902
588935-902
520518-904
520518-905
820999-901

Columns for Analytical HPLC

Normal-Phase Columns
ZORBAX Normal-Phase Columns
For normal-phase chromatography, the Agilent ZORBAX product line offers a choice of bonded
and non-bonded silica packings.

ZORBAX Rx-SIL
• Made from highly pure (>99.995%) porous silica microspheres (pore size is the space
between the solid silica microparticles)

• Available in 1.8 and 5 µm particle sizes
• Stronger than other silica types
• Less acidic than ZORBAX-SIL, lower metal content
• Low acidity and low metal content make ZORBAX Rx-SIL ideal for normal-phase separation
of polar compounds that exhibit poor peak symmetry on more acidic silica

• Useful for very hydrophilic compounds with high organic mobile phases in HILIC mode
ZORBAX Eclipse XDB-CN
• Made from highly pure Rx-SIL
• Excellent choice for normal-phase applications with basic compounds
• Equilibrates more rapidly than ZORBAX Rx-SIL and is used for many of the same normalphase applications

LC AND LC/MS COLUMNS

873

Columns for Analytical HPLC

Pursuit XRs Silica is another choice
for normal-phase chromatography.
For more information,
see page 862–863.

ZORBAX CN
• Cyanopropyldimethylsilane monolayer bonded to ZORBAX SIL
• Equilibrates more rapidly than ZORBAX SIL, and used for many of the same normal-phase
applications

• Less prone to fouling and less water sensitive than silica
ZORBAX NH2
• Amino-propyl silane phase bonded to ZORBAX SIL
• Used for normal-phase and weak anion-exchange, and reversed-phase HPLC
of polar compounds
• Vitamins A and D are separated in the normal-phase mode
• Carbohydrates and sugars are separated in the reversed-phase mode

Column Specifications
Bonded Phase
ZORBAX Rx-SIL
ZORBAX Eclipse
XDB-CN
ZORBAX SIL
ZORBAX CN
ZORBAX NH2

Pore Size
80Å
80Å

Surface Area
180 m2/g
180 m2/g

pH Range
0-8.0
2.0-8.0

Endcapped
No
Yes

70Å
70Å
70Å

300 m2/g
300 m2/g
300 m2/g

0-8.0
2.0-7.0
2.0-7.0

No
Yes
Yes

Carbon Load

7%
4%

High Resolution Normal-Phase Separation
of Octylphenoxy Ethanol Surfactant on ZORBAX CN
Column:

ZORBAX CN
880952-705
4.6 x 250 mm, 5 µm

Mobile Phase:

Primary: Heptane
Secondary: 2-Methoxyethanol/Isopropanol (50/50)
2 mL/min
2-20% Secondary in 10 min., Linear Hold at 20%
50°C
278 nm
Octylphenoxy (polyethylene oxy)
Ethanol Surfactant (n= 10)

Flow Rate:
Gradient:
Temperature:
Detector:
Sample:

0

874

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

5

10

l 01357 522 961

15
Time (min)

4.3%

20
LCNP001

Columns for Analytical HPLC

Normal-Phase Columns Based on ZORBAX Rx-SIL
Hardware Description

Size (mm)

Particle Size (µm)

Rx-SIL
USP L3

Eclipse
XDB-CN
USP L10

Standard Columns (no special hardware required)

Semi-Prep
Analytical
Analytical
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar
Narrow Bore
Rapid Resolution HT, 600 bar
Rapid Resolution HT, 600 bar

9.4 x 250
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 50
3.0 x 100
3.0 x 50
2.1 x 150
2.1 x 100
2.1 x 50

5
5
5
1.8
1.8
1.8
1.8
5
1.8
1.8

880975-201
880975-901
883975-901
828975-901
827975-901
828975-301
827975-301
883700-901
828700-901
827700-901

9.4 x 15
4.6 x 12.5
2.1 x 12.5
9.4 x 15

5
5
5
0

820675-119
820950-919
821125-919
840140-901
820999-901

990967-905*
993967-905*

993700-905*

Guard Columns (hardware required)

Guard Cartridge, 2/pk
Guard Cartridges, 4/pk
Guard Cartridge, 4/pk
Guard Hardware Kit
Guard Hardware Kit

820950-935
821125-935
820999-901

PrepHT Cartridge Columns (require endfittings kit 820400-901)

PrepHT Cartridge

21.2 x 250

7

PrepHT Cartridge

21.2 x 250

7

PrepHT endfittings, 2/pk
PrepHT Guard Cartridge, 2/pk
Guard Cartridge Hardware

877250-101
820400-901

17 x 7.5

5

820212-919
820444-901

*These columns ship containing reversed-phase solvents. Flush with isopropanol before using normal-phase solvents. These columns can also be used in HILIC mode.

LC AND LC/MS COLUMNS

875

Columns for Analytical HPLC

Normal-Phase Columns Based on ZORBAX Original SIL
Hardware Description

Size (mm)

Particle Size
(µm)

SIL
USP L3

CN
USP L10

NH2
USP L8

Carbohydrate
Analysis

840300-908
843300-908

Standard Columns (no special hardware required)

Semi-Prep
Analytical
Analytical
Narrow Bore

9.4 x 250
4.6 x 250
4.6 x 150
2.1 x 50

5
5
5
5

880952-201
880952-701
883952-701

880952-205
880952-705
883952-705

880952-208
880952-708
883952-708
860700-708

9.4 x 15
4.6 x 12.5
2.1 x 12.5
9.4 x 15

5
5
5

820675-119
820950-901

820675-111
820950-905

820675-111
820950-908

820950-908

840140-901
820999-901

840140-901
820999-901

840140-901
820999-901

820888-901

877952-105

877952-108

820400-901

820400-901

Guard Columns (hardware required)

Guard Cartridge, 2/pk
Guard Cartridges, 4/pk
Guard Cartridge, 4/pk
Guard Hardware Kit
Guard Hardware Kit

PrepHT Cartridge Columns (require endfittings kit 820400-901)

PrepHT Cartridge

21.2 x 250

7

PrepHT Cartridge

21.2 x 250

7

17 x 7.5

5

PrepHT endfittings, 2/pk
PrepHT Guard Cartridge, 2/pk

877952-101
820400-901

Guard Cartridge Hardware

876

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Columns for Analytical HPLC

ZORBAX HILIC Plus
• HILIC column for good retention of small, polar analytes
• Based on Eclipse Plus silica for excellent peak shape
• High sensitivity for LC/MS applications
• Recommended for EPA Method 1694
Agilent ZORBAX HILIC Plus columns are for use in hydrophilic interaction chromatography
(HILIC) applications, which are typically used for the retention and resolution of small polar
compounds. HILIC Plus columns are non-bonded silica columns based on the high performance
silica used in ZORBAX Eclipse Plus columns. This silica provides excellent peak shape, critical
for many polar, basic analytes. These columns ship prepared for use in HILIC mode – containing
acetonitrile:water – in order to reduce the extensive equilibration typically required for HILIC
separations. HILIC Plus columns are available in a 3.5 µm particle size for high resolution and
in 2.1 and 4.6 mm ID for compatibility with mass spectrometers or with standard UV detectors.

Column Specifications
Bonded Phase
Non-bonded silica

Pore Size

Surface Area

pH Range

95Å

160 m2/g

0-8.0

Specifications represent typical values only.

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

LC AND LC/MS COLUMNS

877

Columns for Analytical HPLC

Separation of Group 4 Analytes in EPA 1694
on ZORBAX HILIC Plus Column
Column:

ZORBAX HILIC Plus
959793-901
2.1 x 100 mm, 3.5 µm

Mobile Phase:
Flow Rate:
Gradient:

90% Acetontrile:10% Water
0.25 mL/min
Linear gradient to 55% acetonitrile
in 7 min
Held at 55%
25°C

Temperature:

Metformin

Cimetidine
x102
1.00
0.95
0.90
0.85
0.80
0.75
0.70
0.65
0.60
0.55
0.50
0.45
0.40
0.35
0.30
0.25
0.20
0.15
0.10
0.05
0

Albuterol
Ranitidine

x102

Duplicate runs for column USCJP0004;
10 min equilibration between two runs

1.00
0.95
0.90
0.85
0.80
0.75
0.70
0.65
0.60
0.55
0.50
0.45
0.40
0.35
0.30
0.25
0.20
0.15
0.10
0.05
0
0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

7.0

7.5

8.0

8.5

9.0

9.5

10.0 10.5 11.0 11.5 12.0 12.5 13.0 13.5 14.0 14.5 15.0 15.5 16.0 16.5 17.0 17.5 18.0 18.5 19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5

EPA1694

ZORBAX HILIC Plus
Description
Analytical
Analytical
Narrow Bore
Narrow Bore

878

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Size (mm)
4.6 x 100
4.6 x 50
2.1 x 100
2.1 x 50

l 01357 522 961

Particle Size (µm)
3.5
3.5
3.5
3.5

Part No.
959961-901
959943-901
959793-901
959743-901

Columns for Analytical HPLC

Ion Exchange Columns
ZORBAX Ion Exchange Columns – SAX and SCX
• ZORBAX SAX and 300SCX columns are based on rugged ZORBAX silica
• Stable from pH 2-7
• Provide high efficiency, rapid separations
• Compatible with organic mobile phase modifiers
Agilent ZORBAX Strong Ion Exchange columns are available as both Strong Anion Exchange
(SAX) and Strong Cation Exchange (300SCX) columns. Each column is packed with bonded,
5 µm, spherical silica particles for optimum efficiency.
ZORBAX SAX packing has a permanently bonded quaternary amine. A trifunctional organo-silane
reagent is used in producing this packing to maximize its stability with aqueous mobile phases.
This column is ideal for separation of water-soluble compounds such as aromatic and aliphatic
carboxylic acids and sulfonic acids.
ZORBAX SCX packing has 300Å pore size silica particles chemically bonded to an aromatic
sulfonic acid group. This column is used for separations of basic, water-soluble compounds and
bio-molecules.

Column Specifications
Bonded Phase
ZORBAX SAX

Pore
Size
70Å

Surface
Area
300 m2/g

pH Range
2.0-7.0

ZORBAX 300SCX

300Å

50 m2/g

2.0-7.0

Functionality
Quaternary
amine
Sulfonic acid

Max Pressure
350 bar
350 bar

Specifications represent typical values only.

LC AND LC/MS COLUMNS

879

Columns for Analytical HPLC

Cough/Cold Remedies on ZORBAX 300SCX
Column:

ZORBAX 300SCX
880952-704
4.6 x 250 mm, 5 µm

Mobile Phase:
Flow Rate:
Temperature:
Detector:
Sample:

100 mM NaH2PO4 (pH 6.5)
3 mL/min
20°C
210 nm
Cold remedies

1

1.
2.
3.
4.
5.

2
3

0

4

5

2
4
Time (min)

Pyrilamine
Theophylline
Glyceryl Guaicolate
Caffeine
Phenylephrine

LCIE001

ZORBAX Ion Exchange Columns – SAX and SCX
Description
Semi-preparative
Analytical
Analytical
Analytical
Solvent Saver
Narrow Bore
Narrow Bore
Guard Cartridge, 4/pk
Guard Hardware Kit

880

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Size (mm)
9.4 x 250
4.6 x 250
4.6 x 150
4.6 x 50
3.0 x 50
2.1 x 150
2.1 x 50
4.6 x 12.5

Particle Size (µm)
5
5
5
5
5
5
5
6

l 01357 522 961

SAX
880952-203
880952-703
883952-703

820950-903
820888-901

300SCX
880952-204
880952-704
883952-704
846952-704
860700-304
883700-704
860700-704
820950-904
820888-901

Columns for Analytical HPLC

Hi-Plex HPLC Columns
• Preferred separation mechanism for the analysis of carbohydrates and oligosaccharides
• Matched to the USP definitions of media types L17, L19, L34 and L58
• Ideal for isocratic separations using water or dilute acid as the eluent
Hi-Plex columns are ion exchange or ligand exchange columns used predominantly for the
separation of carbohydrates and organic acids. These columns are the preferred separation
mechanism for the analysis of simple sugars, alcohols, oligosaccharides and organic acids in
foodstuffs, but they can be used for the separation of other compounds as well.
The range comprises a 4% cross-linked resin for the analysis of oligosaccharides and an
8% cross-linked resin, with lower exclusion limit, for mono-, di- and tri-saccharide analysis.
For carbohydrate and alcohol investigations, Hi-Plex columns use isocratic conditions with water
as the eluent and temperature as the main variable for control of resolution. The exception is
the Hi-Plex Na (Octo), which is used with sodium hydroxide eluents when pulsed amperometric
detection (PAD) is employed.

Column Specifications
Bonded Phase
Hi-Plex Ca
Hi-Plex Ca USP L19
Hi-Plex Pb
Hi-Plex H for carbohydrates
Hi-Plex H for organic acids
Hi-Plex Ca (Duo)
Hi-Plex K
Hi-Plex Na (Octo)
Hi-Plex Na

Temperature
Range
80-90°C
80-90°C
70-90°C
60-70°C
40-60°C
80-90°C
80-90°C
80-90°C

Flow Rate
(mL/min)
0.6
0.3
0.6
0.6
0.6
0.6
0.6
0.6

80-90°C

0.3

Eluent
Water
Water
Water
Water
Dilute Acid
Water
Water
Water, Sodium
Hydroxide
Water

LC AND LC/MS COLUMNS

881

Columns for Analytical HPLC

Hi-Plex Column Selection
USP methods specify the type of HPLC media and column dimensions which should be used
for the analysis. The Hi-Plex product range has four materials that comply with USP definitions.

Media Type L17
Strong cation exchange resin consisting of sulfonated, cross-linked styrene-divinylbenzene
copolymer in the hydrogen form, 7 to 11 µm in diameter – Hi-Plex H.

Media Type L19
Strong cation exchange resin consisting of sulfonated, cross-linked styrene-divinylbenzene
copolymer in the calcium form, 9 µm in diameter – Hi-Plex Ca and Hi-Plex Ca (Duo).

Media Type L34
Strong cation exchange resin consisting of sulfonated, cross-linked styrene-divinylbenzene
copolymer in the lead form, about 9 µm in diameter – Hi-Plex Pb.

Media Type L58
Strong cation exchange resin consisting of sulfonated, cross-linked styrene-divinylbenzene
copolymer in the sodium form, 6 to 30 µm diameter – Hi-Plex Na and Hi-Plex Na (Octo).
In addition to the standard column sizes, the media is also packed in specific column dimensions
for different USP methods, including sugar alcohol analysis.
For some application areas there are several column options, and the choice of the most
appropriate Hi-Plex media will depend on sample matrix and exact carbohydrate composition.

882

LC AND LC/MS

www.agilent.com/chem/lccolumns
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l 01357 522 961

Columns for Analytical HPLC

Hi-Plex Column Selection
Application Area
USP Methods Specifying L17 Media
USP Methods Specifying L19 Media
USP Methods Specifying L34 Media
USP Methods Specifying L58 Media
Mono- and Disaccharides

Anomer Separations
Organic Acids
Alcohols

Adulteration of Food and Beverages
Food Additives
Dairy Products
Sweetened Dairy Products
Confectionery
Fruit Juice
Wine
Wood Pulp Hydrolysates (cellulose/hemi-cellulose)
Fermentation Monitoring
Oligosaccharides
Samples with High Salt Content (molasses)
Oligosaccharides 90% purity.
Separations up to 2000 mg are possible
depending on the complexity of separation.

0

1

2

3

4
Time (min)

5

6

7
MSD

Vial 2 – 343
Vial 1 – 347
8000000

Vial 3 – 389

1. Nifedipine
2. Nimodine
3. Nisoldipine

6000000
4000000
2000000
0

1

2

3

4
Time (min)

5

6

7
LCPHT01

Amount Nifedipin [mg] Amount Nifmodipin [mg] Amount Nifsoldipin [mg]
Fraction 1
Fraction 2
Fraction 3
Recovery [mg]
Recovery [%]

918

LC AND LC/MS

18.90
0.29
0.49
19.68
101.3

0.11
17.66
1.66
19.43
102.0

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

0.16
0.77
18.36
19.29
101.9

l 01357 522 961

Purity Nifedipin
Purity Nifmodipin
Purity Nifsoldipin

98.6%
94.4%
89.5%

Preparative HPLC Columns and Flash Chromatography

ZORBAX PrepHT columns are designed for rapid scale-up from analytical to preparative scale
without losing resolution. For complex separations for larger columns (21.2 mm ID and higher,
150 mm length and higher), Agilent has carefully chosen the 7 µm particle size to achieve a
balance between high efficiency and high loadability.

Scale-Up from Analytical to Prep ZORBAX SB-C18 Columns
Using the Same Pump
Flow
(mL/min)
100
18
3.5
0.85

Column Size
Column 1 50 x 150 mm
Column 2 21.2 x 150 mm
Column 3 9.4 x 150 mm
Column 4 4.6 x 150 mm

Injection
(µL)
2200
400
80
2.0

Detector Cell
0.3 mm quartz
0.3 mm quartz
0.3 mm quartz
3 mm SST

Part No.
Custom Column
877150-102
883975-202
883975-902

Using the same 1100 pump, a scale-up from 4.6 mm to 50 mm ID was possible without any loss of resolution. This increases
throughput by reducing the time required for redeveloping and adjusting the method.

Scale-Up to PrepHT
2

3500

3

Abundance (mAU)

3000

1. Theobromine
2. Theophylline
3. Caffeine

2500
2000

Column 1

1500

1
Column 2

1000

Column 3

500
Column 4

0
0

2.5

5.0

7.5

10.0
12.5
Time (min)

15.0

17.5

20.0
LCPHT_ScaleUp

LC AND LC/MS COLUMNS

919

Preparative HPLC Columns and Flash Chromatography

ZORBAX PrepHT 80ÅStableBond (require hardware 820400-901)
Particle Size SB-C18
SB-C8
SB-CN
SB-Phenyl
(µm)
USP L1
USP L7
SB-Aq
USP L10
USP L11
7
877250-102 877250-106 877250-114 877250-105 877250-112

Hardware Description
PrepHT Cartridge

Size (mm)
21.2 x 250

PrepHT Cartridge

21.2 x 150

7

877150-102 877150-106 877150-114

PrepHT Cartridge

21.2 x 150

5

870150-902 870150-906 870150-914

PrepHT Cartridge

21.2 x 100

5

870100-902 870100-906 870100-914

PrepHT Cartridge

21.2 x 50

5

870050-902 870050-906 870050-914

PrepHT Guard Cartridge, 2/pk

17 x 7.5

5

820212-920 820212-915 820212-933 820212-933 820212-915

ZORBAX PrepHT 300ÅStableBond (require hardware 820400-901)
Particle Size 300SB-C18
(µm)
USP L1
7
897250-102

300SB-C8
USP L7
897250-106

300SB-C3
USP L56
897250-109

897150-102

897150-106

897150-109

895150-902

895150-906

895150-909

5

895100-902

895100-906

895100-909

5

895050-902

895050-906

895050-909

Hardware Description
PrepHT Cartridge

Size (mm)
21.2 x 250

PrepHT Cartridge

21.2 x 150

7

PrepHT Cartridge

21.2 x 150

5

PrepHT Cartridge

21.2 x 100

PrepHT Cartridge

21.2 x 50

PrepHT Guard Cartridge, 2/pk

17 x 7.5

5

Guard Cartridge Hardware
Includes guard column end fitting, polymeric seal, and seal
insertion tool (seal holder and seal pusher)
PrepHT endfittings, 2/pk

300SB-CN
USP L10
897250-105

820212-921

820212-918

820212-924

820212-924

820444-901

820444-901

820444-901

820444-901

820400-901

820400-901

820400-901

820400-901

ZORBAX PrepHT Original (require hardware 820400-901)
Hardware Description
PrepHT Cartridge

Size (mm)
21.2 x 250

Particle Size ODS (C18) C8
CN
NH2
SIL
(µm)
USP L1
USP L7
USP L10
USP L8
USP L3
7
877952-102 877952-106 877952-105 877952-108 877952-101

PrepHT endfittings, 2/pk

920

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

820400-901 820400-901 820400-901 820400-901 820400-901

l 01357 522 961

Preparative HPLC Columns and Flash Chromatography

ZORBAX PrepHT Eclipse XDB (require hardware 820400-901)
Eclipse
XDB-C18
USP L1
977250-102

Eclipse
XDB-C8
USP L7
977250-106

Hardware Description
PrepHT Cartridge

Size (mm)
21.2 x 250

Particle Size
(µm)
7

PrepHT Cartridge

21.2 x 150

7

977150-102

977150-106

PrepHT Cartridge

21.2 x 150

5

970150-902

970150-906

PrepHT Cartridge

21.2 x 100

5

970100-902

970100-906

PrepHT Cartridge

21.2 x 50

5

970050-902

970050-906

PrepHT Guard Cartridge, 2/pk

17 x 7.5

5

Guard Cartridge Hardware
Includes guard column end fitting, polymeric seal, and seal insertion tool (seal holder and seal pusher)
PrepHT endfittings, 2/pk

820212-925

820212-926

820444-901

820444-901

820400-901

820400-901

Extend-C18
USP L1

ZORBAX PrepHT Bonus-RP and Extend-C18 (require hardware 820400-901)
Hardware Description
PrepHT Cartridge

Size (mm)
21.2 x 250

Particle Size
(µm)
7

Bonus-RP
USP L60
878250-101

PrepHT Cartridge

21.2 x 150

7

878150-101

PrepHT Cartridge

21.2 x 150

5

868150-901

770150-902

PrepHT Cartridge

21.2 x 100

5

868100-901

770100-902

PrepHT Cartridge

21.2 x 50

5

868050-901

770050-902

PrepHT Guard Cartridge, 2/pk

17 x 7.5

5

820212-928

820212-930

Guard Cartridge Hardware
820444-901
Includes guard column end fitting, polymeric seal, and seal insertion tool (seal holder and seal pusher)
PrepHT endfittings, 2/pk
820400-901

820444-901
820400-901

ZORBAX PrepHT Rx-SIL (require hardware 820400-901)
Hardware Description
PrepHT Cartridge

Size (mm)
21.2 x 250

Particle Size
(µm)
7

PrepHT Cartridge

21.2 x 250

7

PrepHT Guard Cartridge, 2/pk

17 x 7.5

5

Guard Cartridge Hardware
Includes guard column end fitting, polymeric seal, and seal insertion tool (seal holder and seal pusher)
PrepHT endfittings, 2/pk

Rx-SIL
USP L3
877250-101

Rx-C18
USP L1
877967-102

820212-919

820212-914

820444-901

820444-901

820400-901

820400-901

ZORBAX PrepHT Accessories
Hardware Description
Guard Cartridge Hardware

Part No.
820444-901

PrepHT endfittings, 2/pk

820400-901

Replacement Seals

820385-901

LC AND LC/MS COLUMNS

921

Preparative HPLC Columns and Flash Chromatography

Agilent Prep LC Columns
• High loadability for maximum sample purification
• Easy scalability from 4.6 up to 50 mm ID for rapid method development
• High throughput 21.2 mm ID cartridges for fast purification
• Exceptional column stability and loadability up to pH 10
Agilent Prep LC columns are designed for high loadability to purify milligram to gram quantities
of products. Preparative sized columns are available in 21.2, 30, and 50 mm internal diameters
with lengths ranging from 50-250 mm. Columns are available in 5 and 10 µm particle sizes
with very high efficiency in every dimension. These column choices accommodate almost
every preparative sample.
Agilent Prep 21.2 mm ID columns are available with Agilent’s Preparative Cartridge Hardware.
This reliable cartridge hardware makes it simple to use columns with different lengths to increase
sample load. Guard columns are easily integrated onto these columns, providing superior
protection of the analysis column. Analytical size 4.6 mm ID scalar columns are available for
method development and optimization prior to scaling up to larger columns. Bulk material
is also available.
Agilent Prep columns are available in a C18 bonded phase suitable for purification of a wide
variety of non-polar and polar compounds. Unbonded silica columns are also available.

Column Specifications
Bonded
Phase
C18
Silica

Pore Size
100Å
100Å

Surface
Area
400 m2/g
400 m2/g

Temp. Limits pH Range
60°C*
2.0-10.0
**
1.0-8.0

Specifications represent typical values only.
*Temperature limits are 60°C up to pH 8, 40°C from pH 8-10.
**Temperature limits for bare silica are determined by the pH of the mobile phase.

922

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Endcapped Carbon Load
Single
24%
N/A
N/A

Preparative HPLC Columns and Flash Chromatography

Superior Loadability on Agilent Prep C18
with Basic Compounds
Column:

Mobile Phase:
Flow Rate:
Sample:

Agilent Prep C18
443905-902
4.6 x 150 mm, 5 µm
50% 0.1%TFA:50% ACN
1 mL/min
10 µL
Doxepin/Amitriptyline
0.5-50 mg/mL

Agilent Prep C18

Competitor W-C18

mAU
mAU

4000

40
35
30
25
20
15
10
5
0

α = 1.6

3500
3000
2500
2000
1500
1000
500
0

Agilent Prep columns show better resolution and
loadability than competitor columns.

0

1

2

3

4 5 6
Time (min)

7

8

α = 1.57

0

9

1

2

3

4 5 6
Time (min)

7

8

9
LCPLC01

Steroids: Easy Scalability
Using Agilent Prep Columns
Column A:
Column B:
Column C:
Column D:
Mobile Phase:
Flow Rate:

Temperature:
Detector:
Sample:

Agilent Prep C18
443905-902
4.6 x 150 mm, 5 µm
443905-102
21.2 x 150 mm, 5 µm
413910-302
30.0 x 150 mm, 10 µm
413910-502
50.0 x 150 mm, 10 µm
55% Water:45% ACN
0.7 mL/min
14.87 mL/min
29.77 mL/min
85.37 mL/min
Ambient
240 nm
2 µL
42.4 µL
170 µL
488 µL

1. Hydrocortisone
2. Testosterone (in MeOH @ 1mg/mL)
A

0

1

2

B

4

2

6

8

10

12

1
2

0

2

C

4

6

8

10

12

1
2

0

2

D

4

6

8

10

12

1

Agilent Prep C18 shows excellent scalability, making method
transfer simple and predictable.

2

0

2

4

6
Time (min)

8

10

12
LCPLC02

LC AND LC/MS COLUMNS

923

Preparative HPLC Columns and Flash Chromatography

Agilent Prep LC Columns
Hardware Description

Size (mm)

Particle Size (µm) C18

Silica

Standard Columns (no special hardware required)

Scalar
Scalar
Scalar
Scalar
Scalar
Scalar
Scalar

4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 250
4.6 x 150
4.6 x 100
4.6 x 50

10
10
10
5
5
5
5

440910-902
443910-902
449910-902
440905-902
443905-902
449905-902
446905-902

440910-901
443910-901
440905-901
443905-901
449905-901
446905-901

PrepHT Cartridge Columns (require endfittings kit 820400-901)*

PrepHT

21.2 x 250

10

410910-102 410910-101

PrepHT

21.2 x 150

10

413910-102 413910-101

PrepHT

21.2 x 50

10

446910-102

PrepHT

21.2 x 150

5

443905-102 443905-101

PrepHT

21.2 x 100

5

449905-102 449905-101

PrepHT

21.2 x 50

5

446905-102 446905-101

PrepHT endfittings, 2/pk

820400-901 820400-901

Standard Columns (no special hardware required)

Prep 30
Prep 30
Prep 30
Prep 30
Prep 30
Prep 50
Prep 50
Prep 50
Prep 50

30.0 x 250
30.0 x 150
30.0 x 100
30.0 x 100
30.0 x 50
50.0 x 250
50.0 x 150
50.0 x 100
50.0 x 100

10
10
10
5
5
10
10
10
5

410910-302
413910-302
419910-302
449905-302
446905-302
410910-502
413910-502
419910-502
449905-502

410910-301
413910-301
419910-301
449905-301
446905-301
410910-501
413910-501
419910-501
449905-501

10

420212-902 420212-901

Guard Columns (hardware required)

PrepHT Guard
21.2 x 10
Cartridges, 2/pk
Guard Cartridge Hardware

820444-901 820444-901

PrepHT External Guard Hardware Kit
Bulk Packing (1kg)

420420-901 420420-901
10

420910-902 420910-901

*All PrepHT cartridge columns require hardware kit P/N 820400-901. If a guard column is desired for the 21.2 mm ID columns,
the PrepHT Guard Hardware Kit, P/N 820444-901, is also required. If the guard column is used on a 30 mm ID column then
the external guard column hardware kit, P/N 420420-901, is required.

924

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Preparative HPLC Columns and Flash Chromatography

High Efficiency Purification
for Small Molecule Separations
• Small column sizes for high-speed media selection, method development and purification
• Comprehensive range of selectivities
• Packed columns and bulk media
Agilent offers a range of high efficiency, small particle silica and polymeric HPLC materials.
These are pre-packed preparative columns and bulk media for reverse phase, normal phase
and ion exchange purification. A range of pore sizes is available, providing maximum capacity
for all applications, from small molecules to biological macromolecules.

Small Molecule Separations
Separation
Hydrophobic

Media Characterization
Highest Mass Loading
Hydrophobic Work Horse

Hydrophilic

Aromatic/Double Bonds
Polar Selectivity
H-bond Accepting
Reverse or Normal-Phase
H-bonding
Normal-Phase Organic Soluble

Extreme Conditions

pH Extremes/High Temperatures

Column
Pursuit XRs 100Å C18
Pursuit XRs 100Å C8
Pursuit 200Å C18
Pursuit 200Å C8
Pursuit 200Å Diphenyl
Polaris 200Å C18-A
Polaris 200Å C8-A
Polaris 200Å C18-Ether
Polaris 200Å C8-Ether
Polaris 200Å NH2
Polaris 200Å Amide-C8
Pursuit XRs Si
Pursuit XRs Diol
PLRP-S 100Å, 8 µm

LC AND LC/MS COLUMNS

925

Preparative HPLC Columns and Flash Chromatography

Pursuit High Efficiency XRs Columns for Small Molecule Separations
Size (mm)
21.2 x 250
21.2 x 250
21.2 x 100
10.0 x 250
10.0 x 250

Particle Size (µm)
10
5
5
10
5

XRs C18
A6002250X212
A6000250X212

XRs C8

XRs Diphenyl

XRs Diol

A6020250X212

A3040250X212

XRs Si
A6004250X100

A6010100X212
A6002250X100
A6000250X100

A6020250X100

High Efficiency Bulk Media

100 g

10

A6002100G

A6012100G

A6004100G

Pursuit High Efficiency Columns for Small Molecule Separations
Size (mm)
21.2 x 250
21.2 x 250
10.0 x 250
10.0 x 250

Particle Size (µm)
10
5
10
5

C18
A6002250X212
A3000250X212
A6002250X100
A3000250X100

C8
A3032250X212
A3030250X212
A3032250X100
A3030250X100

Diphenyl
A3042250X212
A3040250X212
A3042250X100
A3040250X100

PFP
A3050250X212
A3050250X100

Polaris High Efficiency Columns for Small Molecule Separations
Size
(mm)
21.2 x 250
21.2 x 250
10.0 x 250
10.0 x 250

926

Particle
Size
(µm)
10
5
10
5

C18-A
C18-Ether
Amide C18
C8-A
C8-Ether
NH2
Si-A
A2002250X212
A2008250X212
A2004250X212
A2000250X212 A2030250X212 A2006250X212 A2010250X212 A2030250X212 A2013250X212 A2003250X212
A2008250X100
A2000250X100 A2020250X100 A2006250X100 A2010250X100 A2030250X100 A2013250X100

LC AND LC/MS

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l 01357 522 961

Preparative HPLC Columns and Flash Chromatography

Dynamax Preparative HPLC Columns
• Modular design with reusable end fittings reduces hardware costs
• Three internal diameters – 10, 21.4 and 41.4 mm – for easy scale-up
• Integral guard column option for longer column lifetimes with complex samples
The Dynamax preparative column hardware utilizes a patented dynamic axial compression (DAC)
design and is the ideal format for the development and optimization of a high throughput or high
yield purification. The DAC principle of operation maintains packed bed integrity and improves
column performance over an extended period of time with a reduction in operating costs.
Agilent offers Dynamax columns as compression modules (cartridges) onto which separate axial
compression end fittings are fitted. This provides a means of eliminating voids that may form
at the column inlet during use and also enables the end fittings to be reused. When changing
the column it is only necessary to replace the compression module with one of a similar
internal diameter.
There are three options when configuring a Dynamax column. To simplify choice, end fittings
kits are available for each of the configurations. Kit #1 contains the end fittings for using the
Dynamax column without a guard module. Kit #2 contains all the parts needed to operate
with a protective guard module. There is also a guard coupling assembly parts kit to upgrade
Kit #1 to Kit #2. When the guard column is used as a short preparative column only the
standalone guard holder is needed.
SepTech ST60 10-C18 and SepTech ST150 10-C18 media designed for high performance
separations at high capacity are available in the Dynamax format for rapid method development
and small-scale separations.

Dynamax Column Hardware Kits
Description
End fittings kit #1

End fittings kit #2

Guard coupling assembly
Upgrades kit #1 to kit #2
Standalone guard holder

ID (mm)
10
21.4
41.4
10
21.4
41.4
10
21.4
41.4
10
21.4
41.4

Part No.
R000083810
R000083820
R000083840
R000083812
R000083822
R000083842
R000083811
R000083821
R000083841
R000083814
R000083824
R000083844

LC AND LC/MS COLUMNS

927

Preparative HPLC Columns and Flash Chromatography

SepTech C18 Reverse Phase Media
• Symmetrical peaks improve yield of high purity product
• High capacity delivers maximum throughput
• Narrow particle size distribution improves packed bed stability
SepTech media has been developed specifically for prep to process HPLC, from the definition of
the base silica particle, pore sizes, pore volume, specific surface area, mechanical strength and
particle size distribution through to the bonding chemistry, ligand density and end capping. The
result is two products: SepTech ST60 10-C18 – optimized for small molecule purifications, and
SepTech ST150 10-C18 – the preferred option for larger, natural molecules and biomolecules.
The high level of batch-to-batch reproducibility and particle integrity give consistent performance
and ease of column packing, which are essential for minimizing production downtime. SepTech
media helps you meet the demands of a robust and economical process by purifying the
maximum amount of product at the required purity in the shortest period of time.

Column Specifications
Characteristics
Nominal Particle Size
Nominal Pore Size
Nominal Distribution
Shape
Silica Purity
Chemistry
End Capping
Carbon Load
Ligand Coverage
Working pH Range

928

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

SepTech ST60 10-C18
10 µm
60Å
<2 d90/d10
Spherical
99.999%
Octadecyl
Yes
25%
3.5 µmol/m2
1.5-10

l 01357 522 961

SepTech ST150 10-C18
10 µm
150Å
<2 d90/d10
Spherical
99.999%
Octadecyl
Yes
15%
3.8 µmol/m2
1.5-10

Preparative HPLC Columns and Flash Chromatography

SepTech ST60 10-C18
Description
Method Development Column
Dynamax Packed Cartridge Module

Bulk media

Size (mm)
4.6 x 250
10 x 50
10 x 250
21.2 x 50
21.2 x 250
41.4 x 50
41.4 x 250
100 g
1 kg

Part No.
A8060250X046
A8060050DG100
A8060250DM100
A8060050DG214
A8060250DM214
A8060050DG414
A8060250DM414
A80600100G
A8060001KG

Size (mm)
4.6 x 250
10 x 50
10 x 250
21.2 x 50
21.2 x 250
41.4 x 50
41.4 x 250
100 g
1 kg

Part No.
A8150250X046
A8150050DG100
A8150250DM100
A8150050DG214
A8150250DM214
A8150050DG414
A8150250DM414
A81500100G
A8150001KG

SepTech ST150 10-C18
Description
Method Development Column
Dynamax Packed Cartridge Module

Bulk media

Tips & Tools
Don't forget, we have special offers throughout the year.
To learn more, visit www.agilent.com/chem/specialoffers

LC AND LC/MS COLUMNS

929

Preparative HPLC Columns and Flash Chromatography

FlowTrap
• Reduced dry-down times improve productivity
• Desalting in situ preserves compound integrity
• Retentive sorbent handles a wide range of sample pH and pKa
FlowTrap columns contain ultra-retentive, high capacity, and hydrophobic polymeric material
that captures and concentrates small molecules. Once trapped, the desired analyte can be back
eluted using a small volume of volatile organic solvent, affording simple compound isolation.
FlowTrap columns give you excellent retention and easy elution. The efficiency of the packed
bed delivers superior reproducibility and can be used for up to 500 flow-trapping cycles when
run under optimized conditions.
With FlowTrap you can solvent switch from a high volume of water-based HPLC eluent
to a low volume of volatile solvent, dramatically reducing the evaporation times needed
for compound isolation. Ion pairing reagents such as TFA can be removed from the compound
during trapping, allowing the isolation of free-base compounds and reducing the risk of potential
compound hydrolysis.
FlowTrap is available in standard HPLC column hardware covering a range of column sizes that
handle seamless scale-up as compound batch sizes increase. Using FlowTrap columns will help
you dramatically reduce dry-down times, increasing throughput for compound recovery.

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

930

LC AND LC/MS

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l 01357 522 961

Preparative HPLC Columns and Flash Chromatography

FlowTrap
Size (mm)
4.6 x 150
7.5 x 150
10.0 x 150
21.2 x 150

Capacity Range (mg)*
50
50-150
150-200
200-400

Part No.
PL1560-3M07
PL1160-3M07
PL1060-3M07
PL1E60-3M07

*Recommendation only, based on representative loading studies. Capacity will vary according to compound type and eluent
constitution.

Metronidazole TFA removal
Column:

FlowTrap
PL1560-3M07
4.6 x 150 mm, µm

Mobile Phase:

Metronidazole (12 mL) @ 2 mg/mL
in water + 0.1% TFA
Caffeine @ 2 mg/mL in water + 0.1% TFA
Detector:
UV, 280 nm
Wash Conditions: Method A: R.O. water flow rate: 4 mL/min for 4 min
Method B: 2 M NH3, flow rate: (4 mL/min) for 2 min then
R.O. water, flow rate: (4 mL/min) for 4 min
Elution:
100% CH3CN over 5 min flow rate:
(4 mL/min)

Method A
Method B

0

min

35
VLC0013

LC AND LC/MS COLUMNS

931

Preparative HPLC Columns and Flash Chromatography

Load & Lock Preparative HPLC Systems
Agilent offers a complete range of Load & Lock column systems for laboratory and process
preparative LC. They are designed to enable you to easily and quickly pack your own preparative
high efficiency columns. This is the right solution for applications ranging in scale from discovery
(milligrams) to production (multi-kilos) of pharmaceutical compounds, peptides, and natural
products. Our Load & Lock columns have a unique fluid/sample distribution system to
maximize productivity. It is the only system that provides dynamic axial compression (DAC)
and static "locked" axial compression (SAC) and is designed for easy operation to deliver
greater convenience.

Laboratory Load & Lock Columns
• Mobile packing station supports three different column sizes
• Runs on compressed air with no need for a power supply
• Quick and easy packing and unpacking within minutes
Agilent's laboratory scale Load & Lock columns combine excellent packed-bed stability with
enhanced flow distribution to deliver the highest quality purification possible with maximum
speed, flexibility and ease of operation. Three different column sizes are supported: 1 in., 2 in.
and 3 in. ID. Because the station is powered by compressed air, it is the perfect solution for
hazardous environments. The quick-release single bolt clamp offers speedy and easy packing
and unpacking within minutes.

Load & Lock Preparative HPLC Systems
Description
Load & Lock 4001 Column

Load & Lock 4002 Column

Load & Lock 4003 Column

Water Jacket
No
Yes
Spare parts kit
No
Yes
Spare parts kit
No
Yes
Spare parts kit

Mobile packing station (air driven hydraulic)

932

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Size (mm)
25 x 500
25 x 500
50 x 500
50 x 500
75 x 500
75 x 500

Part No.
PCG93LL500X25
PCG93LL500X25WJ
PCG931AAKIT
PCG93LL500X50
PCG93LL500X50WJ
PCG932AAKIT
PCG93LL500X75
PCG93LL500X75WJ
PCG933AAKIT
PCG93LLSTAND123

Preparative HPLC Columns and Flash Chromatography

Flash Chromatography
• Isolate compounds from synthesis mixtures quickly and easily
• Maximize compound purity and recovery with superior purification columns
• Enhance gradient accuracy with solid loading system
Flash chromatography purifies reaction products to isolate the target compound. Flash columns
are designed for purification. Every element has been thought out, custom designed and carefully
manufactured for excellent purification performance, time after time.

SuperFlash Purification Columns
• Sixteen standard sizes plus customized sizes for a wide application range
• Short, wide columns for speed and long, thin columns for resolution
• Flat packaging for stock room organization and supply visibility to maintain inventory
Each element of the SuperFlash compound purification column, with our patented and patentpending technologies, delivers optimal performance, offering maximum recovery of high purity
compounds time after time. Our columns, available in Si 50, Si 35, C18, PLRP-S and SCX for
normal and reverse phase separations, and a variety of other sorbents, eliminate the common
problems of leaking, size limitations, complicated connections and poor compound separation.
Instead, you receive a cost-effective, high performance disposable column specifically designed
for delivering convenient, efficient separations.

LC AND LC/MS COLUMNS

933

Preparative HPLC Columns and Flash Chromatography

Media selection
Normal phase (NP)
Low or medium polarity
Acidic

Acidic alumina (NP)
Reverse phase (RP)
Reverse phase (RP)

High polarity
Acid sensitive

Neutral alumina (NP)

Sample
Low or medium polarity
Neutral
High polarity

Normal phase (NP)
Neutral alumina (NP)
Reverse phase (RP)
Normal phase (NP)

Low or medium polarity

Amine (NP/RP)
Basic alumina (NP)

Basic

Reverse phase (RP)

High polarity

Strong Cation Exchange (SCX)

Standard Female Luer Lock
Easily connects to any system or to a DASi injection cartridge.
Unitized Construction
Patent pending unitized construction hermetically seals the column into a single unit
to eliminate leaking up to 200 psi.
Identification Stripe
Solvent proof (laser etched/no ink) red stripe includes data on column specifications and helpful information for
reordering. Normal-phase columns have a red stripe, while reversed-phase columns have a blue stripe.
Solvent Resistant Column Body
The column body and cap design use a material that does not swell,
warp or crack under prolonged solvent contact.
Dispersion Channels
Load sample evenly onto the column.
Standard Male Luer Lock
Male luer outlet attaches to any system.

934

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Preparative HPLC Columns and Flash Chromatography

Solvent Polarity
Polarity Index at 20°C
Non-polar

Polar

0.0
0.0
0.0
0.2
1.0
1.6
2.8
3.1
3.9
4.0
4.0
4.1
5.1
5.1
5.2
5.8
9.0

Solvent
Heptane
Hexane
Pentane
Cyclohexane
Trichloroethylene
Carbon tetrachloride
di-Ethyl ether
Dichloromethane
Propan-2-ol
Propan-1-ol
Tetrahydrofuran
Chloroform
Acetone
Methanol
Ethanol
Acetonitrile
Water

LC AND LC/MS COLUMNS

935

Preparative HPLC Columns and Flash Chromatography

SuperFlash Notes
This information applies to the following SuperFlash ordering tables.

• Maximum pressure for all columns is 14 bar (200 psi).
• Obey pressure maximum limits marked on every column. Confirm the instrument has been
set to the appropriate maximum pressure before attaching column.

• Dimensions are for sorbent bed diameter x overall column length.
• Flow rates up to 40% higher than the recommended normal operating flow rates may
be used to reduce equilibration times.

• Sample loading values are suggested. Results may vary with specific samples.

Normal Phase (NP)
SuperFlash Si 50
Model
SF10 - 4 g
SF10 - 8 g
SF15 - 12 g
SF15 - 24 g
SF25 - 40 g
SF25 - 60 g
SF25 - 80 g
SF25 - 120 g
SF25 - 160 g
SF40 - 80 g
SF40 - 120 g
SF40 - 150 g
SF40 - 240 g
SF65 - 200 g
SF65 - 400 g
SF65 - 600 g

936

LC AND LC/MS

Diameter x
Length (mm)
14.2 x 95
14.2 x 136
20.8 x 112
20.8 x 175
28.2 x 164
28.2 x 214
28.2 x 280
28.2 x 388
28.2 x 507
40.6 x 158
40.6 x 202
40.6 x 257
40.6 x 371
66 x 156
66 x 256
66 x 365

Particle Size
(µm)
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Flow Rate
(mL/min)
18
18
30
30
40
40
40
40
40
85
85
85
85
100
100
100

l 01357 522 961

Sample Load
40 - 400 mg
80 - 800 mg
120 mg - 1.2 g
240 mg - 2.4 g
400 mg - 4 g
600 mg - 6 g
800 mg - 8 g
1.2 - 12 g
1.6 - 16 g
800 mg - 8 g
1.2 - 11.5 g
1.5 - 15 g
2.4 - 24 g
2 - 20 g
4 - 40 g
6 - 60 g

Unit
8/pk
8/pk
7/pk
7/pk
6/pk
6/pk
6/pk
6/pk
6/pk
4/pk
4/pk
4/pk
4/pk
3/pk
3/pk
3/pk

Part No.
AX1368-8
AX1403-8
AX1369-7
AX1404-7
AX1281-6
AX1212-6
AX1213-6
AX1214-6
AX1215-6
AX1356-4
AX1216-4
AX1217-4
AX1218-4
AX1357-3
AX1219-3
AX1220-3

Preparative HPLC Columns and Flash Chromatography

SuperFlash Si 35
Model
SF10 - 4 g
SF10 - 8 g
SF15 - 12 g
SF15 - 24 g
SF25 - 40 g
SF25 - 60 g
SF25 - 80 g
SF25 - 120 g
SF25 -160 g
SF40 - 80 g
SF40 - 115 g
SF40 - 150 g
SF40 - 240 g
SF65 - 200 g
SF65 - 400 g
SF65 - 600 g

Diameter x
Length (mm)
14.2 x 95
14.2 x 136
20.8 x 112
20.8 x 175
28.2 x 164
28.2 x 215
28.2 x 280
28.2 x 388
40.6 x 507
40.6 x 158
40.6 x 202
40.6 x 257
40.6 x 371
66 x 156
66 x 256
66 x 365

Particle Size
(µm)
35
35
35
35
35
35
35
35
35
35
35
35
35
35
35
35

Flow Rate
(mL/min)
18
18
30
30
40
40
40
40
40
85
85
85
85
100
100
100

Sample Load
40 - 400 mg
80 - 800 mg
120 mg - 1.2 g
240 mg - 2.4 g
400 mg - 4 g
600 mg - 6 g
800 mg - 8 g
1.2 - 12 g
1.6 - 16 g
800 mg - 8 g
1.2 - 11.5 g
1.5 - 15 g
2.4 - 24 g
2 - 20 g
4 - 40 g
6 - 60 g

Unit
8/pk
8/pk
7/pk
7/pk
6/pk
6/pk
6/pk
6/pk
6/pk
4/pk
4/pk
4/pk
4/pk
3/pk
3/pk
3/pk

Part No.
AX1370-8
AX1407-8
AX1371-7
AX1408-7
AX1393-6
AX1292-6
AX1293-6
AX1294-6
AX1295-6
AX1405-4
AX1296-4
AX1297-4
AX1298-4
AX1406-3
AX1299-3
AX1300-3

SuperFlash Aminopropyl – NH2
Model
SF10 - 5 g
SF10 - 10 g
SF15 - 15 g
SF15 - 30 g
SF25 - 50 g
SF25 - 75 g
SF25 - 100 g
SF25 - 150 g
SF25 -200 g
SF40 - 100 g
SF40 - 150 g
SF40 - 200 g
SF40 - 300 g
SF65 - 250 g
SF65 - 500 g
SF65 - 750 g

Diameter x
Length (mm)
14.2 x 96
14.2 x 136
20.8 x 113
20.8 x 174
28.2 x 163
28.2 x 220
28.2 x 277
28.2 x 391
28.2 x 506
40.6 x 159
40.6 x 207
40.6 x 255
40.6 x 379
66 x 157
66 x 262
66 x 365

Particle Size
(µm)
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40

Flow Rate
(mL/min)
18
18
30
30
40
40
40
40
40
85
85
85
85
100
100
100

Unit
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk

Part No.
AX1374-1
AX1511-1
AX1375-1
AX1512-1
AX1311-1
AX1376-1
AX1377-1
AX1378-1
AX1379-1
AX1380-1
AX1316-1
AX1317-1
AX1381-1
AX1382-1
AX1319-1
AX1383-1

LC AND LC/MS COLUMNS

937

Preparative HPLC Columns and Flash Chromatography

Strong Cation Exchange (SCX)
SuperFlash SCX
Model
SF10 - 5 g
SF10 - 8 g
SF15 - 14 g
SF15 - 25 g
SF25 - 45 g
SF25 - 70 g
SF25 - 80 g
SF25 - 120 g
SF25 - 160 g
SF40 - 80 g
SF40 - 125 g
SF40 - 160 g
SF40 - 245 g
SF65 - 250 g
SF65 - 440 g
SF65 - 650 g

938

LC AND LC/MS

Diameter x
Length (mm)
14.2 x 49
14.2 x 81
20.8 x 63
20.8 x 114
28.2 x 114
28.2 x 186
28.2 x 206
28.2 x 308
28.2 x 414
40.6 x 99
40.6 x 153
40.6 x 208
40.6 x 299
66 x 118
66 x 204
66 x 302

Particle Size
(µm)
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Flow Rate
(mL/min)
18
18
30
30
40
40
40
40
40
85
85
85
85
100
100
100

l 01357 522 961

Unit
8/pk
8/pk
7/pk
7/pk
6/pk
6/pk
6/pk
6/pk
6/pk
4/pk
4/pk
4/pk
4/pk
3/pk
3/pk
3/pk

Part No.
AX2130-8
AX2135-8
AX2140-7
AX2145-7
AX2150-6
AX2155-6
AX2160-6
AX2165-6
AX2170-6
AX2175-4
AX2180-4
AX2185-4
AX2190-4
AX2195-3
AX2200-3
AX2205-3

Preparative HPLC Columns and Flash Chromatography

Reversed Phase (RP)
SuperFlash PLRP-S
Model
SF10 - 2.5 g
SF10 - 4 g
SF15 - 7 g
SF15 - 13 g
SF25 - 24 g
SF25 - 38 g
SF25 - 42 g
SF25 - 63 g
SF25 - 85 g
SF40 - 42 g
SF40 - 65 g
SF40 - 90 g
SF40 - 130 g
SF65 - 133 g
SF65 - 230 g
SF65 - 340 g

Diameter x
Length (mm)
14.2 x 95
14.2 x 127
20.8 x 112
20.8 x 163
28.2 x 163
28.2 x 235
28.2 x 255
28.2 x 357
28.2 x 463
40.6 x 148
40.6 x 202
40.6 x 257
40.6 x 348
66 x 170
66 x 256
66 x 354

Particle Size
(µm)
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50

Flow Rate
(mL/min)
12
12
20
20
30
30
30
30
30
50
50
50
50
65
65
65

Sample Load
15 - 60 mg
25 - 100 mg
41 - 167 mg
75 - 300 mg
138 - 500 mg
188 - 750 mg
250 mg - 1 g
375 mg - 1.5 g
500 mg - 2 g
250 mg - 1 g
375 mg - 1.5 g
500 mg - 2 g
750 mg - 3 g
750 mg - 3 g
1.4 - 5.4 g
2-8g

Unit
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk

Part No.
AX2250-1
AX2255-1
AX2260-1
AX2265-1
AX2270-1
AX2275-1
AX2280-1
AX2285-1
AX2290-1
AX2295-1
AX2300-1
AX2305-1
AX2310-1
AX2315-1
AX2320-1
AX2325-1

Diameter x
Length (mm)
14.2 x 95
14.2 x 127
20.8 x 112
20.8 x 163
28.2 x 163
28.2 x 235
28.2 x 255
28.2 x 357
28.2 x 463
40.6 x 148
40.6 x 202
40.6 x 257
40.6 x 348
66 x 172
66 x 256
66 x 354

Particle Size
(µm)
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50

Flow Rate
(mL/min)
12
12
20
20
30
30
30
30
30
50
50
50
50
65
65
65

Sample Load
15 - 60 mg
25 - 100 mg
41 - 167 mg
75 - 300 mg
138 - 500 mg
188 - 750 mg
250 mg - 1 g
375 mg - 1.5 g
500 mg - 2 g
250 mg - 1 g
375 mg - 1.5 g
500 mg - 2 g
750 mg - 3 g
750 mg - 3 g
1.4 - 5.4 g
2-8g

Unit
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk
1/pk

Part No.
AX1372-1
AX1409-1
AX1373-1
AX1410-1
AX1394-1
AX1302-1
AX1303-1
AX1304-1
AX1305-1
AX1411-1
AX1306-1
AX1307-1
AX1308-1
AX1412-1
AX1309-1
AX1310-1

SuperFlash C18
Model
SF10 - 5 g
SF10 - 10 g
SF15 - 16 g
SF15 - 30 g
SF25 - 55 g
SF25 - 75 g
SF25 - 100 g
SF25 - 150 g
SF25 - 200 g
SF40 - 100 g
SF40 - 150 g
SF40 - 205 g
SF40 - 300 g
SF65 - 300 g
SF65 - 540 g
SF65 - 800 g

LC AND LC/MS COLUMNS

939

Preparative HPLC Columns and Flash Chromatography

Normal Phase (NP) Alumina
SuperFlash Alumina
Model
SF10 - 8 g
SF10 - 16 g
SF15 - 24 g
SF15 - 48 g
SF25 - 80 g
SF25 - 120 g
SF25 - 160 g
SF25 - 240 g
SF25 - 320 g
SF40 - 160 g
SF40 - 230 g
SF40 - 300 g
SF40 - 480 g
SF65 - 400 g
SF65 - 800 g
SF65 - 1200 g

Diameter x Particle Size
Length (mm)
(µm)
14.2 x 95
125
14.2 x 136
125
20.8 x 112
125
20.8 x 175
125
28.2 x 163
125
28.2 x 215
125
28.2 x 280
125
28.2 x 388
125
28.2 x 507
125
40.6 x 158
125
40.6 x 214
125
40.6 x 256
125
40.6 x 388
125
66 x 157
125
66 x 262
125
66 x 365
125

Sample Load
80 - 400 mg
150 - 750 mg
230 mg - 1.2 g
450 mg - 2.2 g
750 mg - 2.2 g
1.1 - 5.5 g
1.5 - 7.5 g
2.2 - 11 g
3 - 15 g
1.5 - 7.5 g
2.2 - 11 g
2.8 - 10 g
4.5 - 22.5 g
3.7 - 18.5 g
7.5 - 37.5 g
11.2 - 56 g

Unit
8/pk
8/pk
7/pk
7/pk
6/pk
6/pk
6/pk
6/pk
6/pk
4/pk
4/pk
4/pk
4/pk
3/pk
3/pk
3/pk

Alumina Neutral
AX1448-8
AX1477-8
AX1466-7
AX1468-7
AX1449-6
AX1481-6
AX1483-6
AX1462-6
AX1485-6
AX1487-4
AX1489-4
AX1438-4
AX1473-4
AX1463-3
AX1491-3
AX1493-3

Alumina Acidic
AX1474-8
AX1494-8
AX1495-7
AX1496-7
AX1497-6
AX1498-6
AX1499-6
AX1500-6
AX1501-6
AX1502-4
AX1503-4
AX1504-4
AX1505-4
AX1506-3
AX1507-3
AX1508-3

Tips & Tools
Agilent offers a variety of e-Seminars and on-site training
to help you learn how to be a more effective chromatographer.
For more information, visit www.agilent.com/chem/education

940

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Alumina Basic
AX1450-8
AX1476-8
AX1467-7
AX1469-7
AX1478-6
AX1480-6
AX1482-6
AX1464-6
AX1484-6
AX1486-4
AX1488-4
AX1437-4
AX1479-4
AX1465-3
AX1490-3
AX1492-3

Preparative HPLC Columns and Flash Chromatography

Flash F75/F150 Cartridges
• Available in a variety of sizes for development systems
• Convenient sorbents to meet your needs
• Consistent packing for less channelling and fraction dilution
If you regularly purify more than a few grams of compound, Flash F75/F150 cartridges deliver
the convenience and compatibility you need. The cartridges are packed with silica for normal phase
separations and silica C18 for reverse phase purifications. For development scale they are available
in different bed diameters and bed masses to provide solutions for a range of sample sizes.

Flash F75 Cartridges
Model
F75S - 200 g
F75S - 200 g
F75S - 200 g
F75S - 200 g
F75S - 300 g
F75M - 400 g
F75M - 400 g
F75M - 400 g
F75M - 400 g
F75M - 600 g
F75L - 800 g
F75L - 800 g
F75L - 800 g
F75L - 800 g
F75L - 1.2 kg
F75XL - 1.6 kg

Sorbent
Si 50
Si 50
Si 35
Si 35
C18
Si 50
Si 35
Si 35
Si 35
C18
Si 50
Si 50
Si 35
Si 35
C18
Si 50

Unit
2/pk
10/pk
2/pk
10/pk
1/pk
2/pk
10/pk
2/pk
10/pk
1/pk
2/pk
10/pk
2/pk
10/pk
1/pk
2/pk

Part No.
AX0346-2
AX0346-10
AX1363-2
AX1363-10
AX0349-1
AX0347-2
AX0347-10
AX1364-2
AX1364-10
AX0350-1
AX0348-2
AX0348-10
AX1352-2
AX1352-10
AX0351-1
AX1178-2

Unit
2/pk
10/pk
2/pk
10/pk
1/pk
2/pk
10/pk
2/pk
10/pk
1/pk

Part No.
AX0355-2
AX0355-10
AX1360-2
AX1360-10
AX0357-1
AX0356-2
AX0356-10
AX1361-2
AX1361-10
AX0414-1

Flash F150 Cartridges
Model
F150M - 2.5 kg
F150M - 2.5 kg
F150M - 2.5 kg
F150M - 2.5 kg
F150M - 3.9 kg
F150L - 5 kg
F150L - 5 kg
F150L - 5 kg
F150L - 5 kg
F150L - 9 kg

Sorbent
Si 50
Si 50
Si 35
Si 35
C18
Si 50
Si 50
Si 35
Si 35
C18

LC AND LC/MS COLUMNS

941

Preparative HPLC Columns and Flash Chromatography

DASi Sample Loading Module
• For even loading of low solubility and high viscosity compounds
• Modules are available in three sizes to match your sample needs
• Adjustable plunger eliminates dead volume and maintains gradient accuracy
• Provides security as a guard column for high-cost, specialty-sorbent columns
DASi Module Kits
Description
DASi 12 module kit
Includes five empty cartridges and two Si 50, 5 g packed cartridges
DASi 35 module kit
Includes five empty cartridges, two Si 50, 5 g packed cartridges and two Si 50,
10 g packed cartridges
DASi 65 module kit
Includes five empty cartridges, two Si 50, 5 g packed cartridges, two Si 50,
15 g packed cartridges and two Si 50, 25 g packed cartridges
DASi 12, 35 and 65 module kit

Part No.
AX1238-1
AX1237-1

AX1236-1

AX1239-1

Each module kit contains plunger assembly and appropriate DASi Si Cartridge Sampler Kit

DASi Si Cartridge Sampler Pack
Description
DASi 12 cartridge sampler pack
DASi 35 cartridge sampler pack
DASi 65 cartridge sampler pack

DASi Module (showing one pre-packed cartridge)
Standard Female Luer Lock
Easily connect the DASi to any system with a female Luer lock top fitting.
Patent Pending Locking Mechanism
Easily push piston down. Assembly will remain in position until released.
Adjustable Plunger Head
Eliminates dead volume to maintain the superior gradient accuracy of the 971-FP instrument
(especially important for DCM/methanol solvent combination).
Sample Cartridge
Solvent compatible cartridge body does not swell, warp, or crack under prolonged solvent contact.
Dispersion Channels
Distribute solvent evenly on the sample bed for tight, thin separation bands.
Standard Male Luer Lock
Male Luer outlet attaches to any system or to the top of a SuperFlash column.

942

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Part No.
AX1266-1
AX1263-1
AX1252-1

Preparative HPLC Columns and Flash Chromatography

Flash Purification
• Excellent purification performance of UV-active compounds at different wavelengths
• Eliminates uncontrolled sample loss to ensure sample security and retention
• Method-guiding functionality optimizes solvent, column and gradient options to increase
efficiency and flexibility
• Ready-to-Run technology reduces downtime

The 971-FP system enhances productivity through its ready-to-run technology that eliminates
warmup time, performs self diagnostics to ensure proper operation, supplies helpful navigation
run start software and introduces walk-away start features like system auto-prime and sample
auto-inject. The instrument incorporates the latest compound separation innovations, and
contains new sample security and retention technology.

Solutions
Description
971-FP multiple wavelength UV flash chromatography workstation
Includes advanced features pack (AFP)
971-FP single wavelength UV flash chromatography workstation

Part No.
AX1600-1
AX1605-1

LC AND LC/MS COLUMNS

943

Preparative HPLC Columns and Flash Chromatography

Instrument Supplies
Agilent offers several accessories to support the 971-FP, including a Multi-column Controller
to connect additional stations for uninterrupted column operation. The Advanced Feature
Package (AFP) offers uninterrupted solvent supply, waste level monitoring and feedback,
Guide Me functionality and dynamic run queues for multi-column control capacity. A high-speed
processor and advanced operating software are integral to the AFP. The integral fume enclosure
traps solvent fumes for use in areas without hoods (requires a 4 in. or greater exhaust ventilation
connection), and the solvent bottle safety tray provides additional support of storing 4 L
solvent bottles.

Instrument Supplies
Description
MCC2 – Multi-column controller
Advanced feature package (AFP)
Integral fume enclosure
Solvent bottle safety tray

Part No.
AX1426-1
AX1440-1
AX1429-1
AX1441-1

Accessory Racks
A variety of accessory racks for the 971-FP, all with radio frequency identification (RFID),
is available.

Accessory Racks
Description
13 x 100 mm rack, holds 90 tubes
16 x 100 mm rack, holds 60 tubes
16 x 150 mm rack, holds 60 tubes
18 x 150 mm rack, holds 40 tubes
25 x 150 mm rack, holds 24 tubes

944

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Part No.
AX1442-1
AX1443-1
AX1444-1
AX1446-1
AX1447-1

GPC/SEC Columns and Calibrants

GPC/SEC COLUMNS AND CALIBRANTS
Agilent delivers leading solutions for characterizing and separating polymers by GPC/SEC.
We manufacture all components for accurate polymer analysis, including columns and standards.
With the addition of Varian in 2010, Agilent greatly expanded its GPC/SEC portfolio to include
the highly respected PLgel, PolarGel, PlusPore, and PL aquagel-OH column families, as well as
an extensive line of polymer standards for GPC/SEC.
If you're currently using one of these part numbers for GPC/SEC columns or standards,
reorder using the new part number listed below:

Cross Reference Guide for GPC/SEC Columns & Standards
If you're using...
Part No.

Reorder this...

Description

Size (mm)

New
Part No.

PLgel 10 µm guard
PLgel 5 µm guard
PLgel 10 µm MIXED-B
PLgel 10 µm 50Å
PLgel 10 µm 100Å
PLgel 10 µm 500Å
PLgel 10 µm 103Å
PLgel 10 µm 104Å
PLgel 10 µm 105Å
PLgel 10 µm 106Å
PLgel 20 µm MIXED-A
PLgel 3 µm MIXED-E
PLgel 5 µm MIXED-C
PLgel 5 µm MIXED-D
PLgel 5 µm 50Å
PLgel 5 µm 100Å
PLgel 5 µm 500Å
PLgel 5 µm 103Å
PLgel 5 µm 104Å
PLgel 5 µm 105Å

7.5 x 50
7.5 x 50
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300

PL1110-1120
PL1110-1520
PL1110-6100
PL1110-6115
PL1110-6120
PL1110-6125
PL1110-6130
PL1110-6140
PL1110-6150
PL1110-6160
PL1110-6200
PL1110-6300
PL1110-6500
PL1110-6504
PL1110-6515
PL1110-6520
PL1110-6525
PL1110-6530
PL1110-6540
PL1110-6550

Organic GPC

79911GP-110
79911GP-510
79911GP-MXB
79911GP-100
79911GP-101
79911GP-102
79911GP-103
79911GP-104
79911GP-105
79911GP-106
79911GP-MXA
79911GP-MXE
79911GP-MXC
79911GP-MXD
79911GP-500
79911GP-501
79911GP-502
79911GP-503
79911GP-504
79911GP-505

(Continued)

LC AND LC/MS COLUMNS

945

GPC/SEC Columns and Calibrants

Cross Reference Guide for GPC/SEC Columns & Standards
If you're using...
Part No.

Reorder this...

Description

Size (mm)

New
Part No.

7.5 x 300
7.5 x 50
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300

PL1120-6830
PL1149-1840
PL1149-6800
PL1149-6840
PL1149-6850
PL1149-6860

Aqueous SEC of Polymers

79911GF-083
79911GF-080
79911GF-MXA
79911GF-084
79911GF-085
79911GF-086

PL aquagel-OH 30 8 µm
PL aquagel-OH 8 µm guard
PL aquagel-OH MIXED-H 8 µm
PL aquagel-OH 40 8 µm
PL aquagel-OH 50 8 µm
PL aquagel-OH 60 8 µm

Polymer Standards for GPC/SEC

79911-60500
79911-60501
79911-60502
5064-8281
1535-4545
5064-8280
1535-4546

946

LC AND LC/MS

S-L-10 polystyrene calibration kit, 10 x 0.5 g
S-M-10 polystyrene calibration kit, 10 x 0.5 g
S-H-10 polystyrene calibration kit, 10 x 0.5 g
EasiVial PS-H, pre-weighted calibration kit
Polyethylene glycol/oxide calibration kits,
PEG-10, 10 x 0.5 g
EasiVial PEG/PEO, pre-weighted calibration kit
Polyacrylic acid - Na salt calibration kit,
PAA-10, 10 x 0.2 g

www.agilent.com/chem/lccolumns
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l 01357 522 961

PL2010-0101
PL2010-0100
PL2010-0103
PL2010-0201
PL2070-0100
PL2080-0201
PL2140-0100

GPC/SEC Columns and Calibrants

Organic GPC
PLgel GPC Columns
• Robust performance under the most exacting conditions
• Temperature stability up to 220°C
• Solvent compatibility allows easy and rapid transfer between solvents of varying polarity
PLgel materials have high pore volume and high efficiency to maximize resolution. Their
unequalled solvent compatibility makes for easy transfer between polar and non-polar eluents,
and outstanding physical rigidity provides extended lifetimes that minimize downtime.
The key to successful GPC separations is the correct choice of columns. The comprehensive
range of PLgel products has been designed to cover virtually all organic solvent-based polymer
analysis application areas, and to make selection of the correct column, solvent and calibration
standard fast and reliable.
PLgel is a highly cross-linked, porous polystyrene/divinylbenzene matrix, which is recognized as a
market leader in GPC column technology. PLgel is manufactured to ISO 9001:2000 and benefits
from comprehensive QC/QA for total reproducibility, batch-to-batch and column-to-column.

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

LC AND LC/MS COLUMNS

947

GPC/SEC Columns and Calibrants

Solvent Compatibility
Solvent Polarity
6.0
7.3
8.2
8.9
9.1
9.1
9.3
9.3
9.7
9.8
9.9
10.0
10.0
10.2
10.2
10.7
10.8
11.3
12.0
12.1

Solvent
Perfluoroalkane
Hexane
Cyclohexane
Toluene
Ethyl acetate
Tetrahydrofuran (THF)
Chloroform
Methyl ethyl ketone (MEK)
Dichloromethane
Dichloroethene
Acetone
o-Dichlorobenzene (o-DCB)
Trichlorobenzene (TCB)
m-Cresol
o-Chlorophenol (o-CP)
Pyridine
Dimethyl acetamide (DMAc)
n-Methyl pyrolidone (NMP)
Dimethyl sulfoxide (DMSO)
Dimethyl formamide (DMF)

Tips & Tools
Don't forget, we have special offers throughout the year.
To learn more, visit www.agilent.com/chem/specialoffers

948

LC AND LC/MS

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l 01357 522 961

GPC/SEC Columns and Calibrants

PLgel MIXED Columns
The PLgel MIXED range greatly simplifies column selection for easy decision making. Using these mixed columns you can eliminate mismatched
column sets and spurious peaks for more reliable results. Every column contains a mixture of individual pore size materials, accurately blended to
cover a specified broad range of molecular weight with a linear calibration to eliminate column mismatch. Simply add extra columns for even
greater resolution.

Column Specifications

Column
MIXED-A

Linear MW
Operating Range
(g/mol)
2,000-40,000,000

Guaranteed
Column
Efficiency
> 17,000 p/m

MIXED-B

500-10,000,000

> 35,000 p/m

MIXED-C

200-2,000,000

> 50,000 p/m

MIXED-D

200-400,000

> 50,000 p/m

MIXED-E

up to 30,0000

7.5 x 300 mm:
> 80,000 p/m
4.6 x 250 mm:
> 70,000 p/m

Typical
Pressure
1 mL/min (7.5 mm ID):
≈ 3 bar (44 psi) per 300 mm
0.3 mL/min (4.6 mm ID):
≈ 2.4 bar (35 psi) per 250 mm
(THF @ 20°C, TCB @ 140°C)
1 mL/min (7.5 mm ID):
≈ 10 bar (145 psi) per 300 mm
0.3 mL/min (4.6 mm ID):
≈ 8 bar (116 psi) per 250 mm
(THF @ 20°C, TCB @ 140°C)
1 mL/min (7.5 mm ID):
≈ 30 bar (435 psi) per 300 mm
0.3 mL/min (4.6 mm ID):
≈ 24 bar (348 psi) per 250 mm
(THF @ 20°C, TCB @ 140°C)
1 mL/min (7.5 mm ID):
≈ 30 bar (435 psi) per 300 mm
0.3 mL/min (4.6 mm ID):
≈ 24 bar (348 psi) per 250 mm
(THF @ 20°C, TCB @ 140°C)
1 mL/min (7.5 mm ID):
≈ 50 bar (725 psi) per 300 mm
0.3 mL/min (4.6 mm ID):
≈ 42 bar (609 psi) per 250 mm
(THF @ 20°C)

Maximum
Flow Rate
7.5 mm ID:
1.5 mL/min
4.6 mm ID:
0.5 mL/min

Maximum
Pressure
150 bar
(2175 psi)

Maximum
Temperature
220°C

7.5 mm ID:
1.5 mL/min
4.6 mm ID:
0.5 mL/min

150 bar
(2175 psi)

220°C

7.5 mm ID:
1.5 mL/min
4.6 mm ID:
0.5 mL/min

150 bar
(2175 psi)

150°C

7.5 mm ID:
1.5 mL/min
4.6 mm ID:
0.5 mL/min

150 bar
(2175 psi)

150°C

7.5 mm ID:
1.5 mL/min
4.6 mm ID:
0.5 mL/min

180 bar
(2611 psi)

110°C

LC AND LC/MS COLUMNS

949

GPC/SEC Columns and Calibrants

PLgel MIXED Column Selection Guide
UHMW polymer distributions

PLgel MIXED-A, 20 µm

High MW polymers, demanding eluents

PLgel MIXED-B, 10 µm

Mid range MW polymers, high resolution

PLgel MIXED-C, 5 µm

Resins, condensation polymers

PLgel MIXED-D, 5 µm

Low MW resins, prepolymers

PLgel MIXED-E, 3 µm
102

107

MW

PLgel MIXED Gel Calibration Curves
MIXED gel calibration curves are designed to be linear over a specified molecular weight range, ensuring that the same degree of resolution is
achieved across the full operating range of the column. The particle size of the packing and porosity of a particular MIXED gel column are carefully
matched to the MW range and application, thus optimizing performance and eliminating the effects of shear degradation. Resolution in GPC is
controlled by the slope of the calibration curve and the particle size of the packing material. Agilent has scientifically determined the minimum
number of MIXED gel columns required to perform accurate MWD determinations based on specific resolution (Rsp). Thus you can have complete
confidence in the accuracy and precision of the calculated data.

Key
MIXED-A, 20 µm

10 M

Polystyrene Molecular Weight

MIXED-B, 10 µm
MIXED-C, 5 µm
MIXED-D, 5 µm
MIXED-E, 3 µm

1K

4

Elution Volume (mL)

10
VLC0014

Reference: Meehan, E. (1998) Size exclusion chromatography columns from Polymer Laboratories. In:
Chi-San Wu (Ed.) Column Handbook for Size Exclusion Chromatography. Academic Press, New York, USA.

950

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

GPC/SEC Columns and Calibrants

PLgel MIXED Columns
Description
PLgel 20 µm MIXED-A
PLgel 10 µm MIXED-B
PLgel 5 µm MIXED-C
PLgel 5 µm MIXED-D
PLgel 3 µm MIXED-E

Size (mm)
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300

Part No.
PL1110-6200
PL1110-6100
PL1110-6500
PL1110-6504
PL1110-6300

Particle Size (µm)
20
10
5
3

Part No.
PL1110-1220
PL1110-1120
PL1110-1520
PL1110-1320

PLgel MIXED Guards
Size (mm)
7.5 x 50
7.5 x 50
7.5 x 50
7.5 x 50

LC AND LC/MS COLUMNS

951

GPC/SEC Columns and Calibrants

PLgel MIXED-LS Columns
• Obtain an instant improvement in data quality
• No need for conditioning, saving time and solvent costs
• Maximize the potential of light scattering detectors
The PLgel MIXED-LS series is a PS/DVB packing using an innovative proprietary suspension
polymerization technique to virtually eliminate nano-particle leakage. A startling improvement is
achieved immediately in the quality of light scattering data obtained with PLgel MIXED-LS
columns in place of conventional GPC columns. The light scattering chromatograms shown here
were obtained after flushing the columns for one hour in THF at 1 mL/min. A polystyrene
standard (Mp 210,000) was injected at 1 mg/mL in order to illustrate the dramatic improvement
in signal-to-noise with the PLgel MIXED-LS column.
The performance of PLgel MIXED-LS columns has been matched to PLgel 20 µm MIXED-A and
PLgel 10 µm MIXED-B columns in terms of calibration, column efficiency, wide solvent
compatibility and operating temperature. MIXED-LS are also ideal for online viscosity detection,
minimizing the risk of capillary blockage, and can be used with regular PLgel guard columns that
are packed with rigid low pore size gels with no particle bleed.

PLgel MIXED-LS Columns
Description
PLgel 10 µm MIXED-B LS
PLgel 10 µm guard
PLgel 20 µm MIXED-A LS
PLgel guard 20 µm

952

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Size (mm)
7.5 x 300
7.5 x 50
7.5 x 300
7.5 x 50

Linear MW
Guaranteed
Operating Range Efficiency
(g/mol) (PS)
(p/m)
500-10,000,000
> 35,000
2,000-40,000,000

l 01357 522 961

> 17,000

Part No.
PL1110-6100LS
PL1110-1120
PL1110-6200LS
PL1110-1220

GPC/SEC Columns and Calibrants

Conventional GPC column
Column:

Conventional GPC column

Mobile Phase:
Flow Rate:
Detector:

THF
1.0 mL/min
LS

60

min

60

min

70

PLgel LS column
Column:

PLgel 10 µm MIXED-B LS
PL1110-6100LS
7.5 x 300 mm, 10 µm

Mobile Phase:
Flow Rate:
Detector:

THF
1.0 mL/min
LS

70
VLC0015

LC AND LC/MS COLUMNS

953

GPC/SEC Columns and Calibrants

PLgel MiniMIX Columns
• Use about 70% less solvent and save money
• Store less solvent and increase operator safety
• High performance comparable to Agilent's conventional ID columns
For reduced solvent cost and consumption, use industry standard PLgel MiniMIX mixed gel
columns in 250 x 4.6 mm narrow bore dimensions. These narrow bore columns offer high
performance, excellent solvent compatibility and mechanical stability. PLgel MiniMIX columns
can be used with conventional GPC equipment.
To maintain the same linear velocity through the column, the volumetric flow rate must be
reduced to 0.3 mL/min in line with the column cross sectional area, resulting in significantly
lower solvent consumption. Sample loading should also be scaled down in line with reduced
column volume, and system dead volume should be minimized to avoid excessive band
broadening.

PLgel MiniMIX Columns
Description
PLgel 20 µm MiniMIX-A
PLgel 20 µm MiniMIX-A guard
PLgel 10 µm MiniMIX-B
Plgel 10 µm MiniMIX-B guard
PLgel 5 µm MiniMIX-C
PLgel 5 µm MiniMIX-C guard
PLgel 5 µm MiniMIX-D
PLgel 5 µm MiniMIX-D guard
PLgel 3 µm MiniMIX-E
PLgel 3 µm MiniMIX-E guard

954

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Size (mm)
4.6 x 250
4.6 x 50
4.6 x 250
4.6 x 50
4.6 x 250
4.6 x 50
4.6 x 250
4.6 x 50
4.6 x 250
4.6 x 50

Linear MW
Guaranteed
Operating Range Efficiency
(g/mol) (PS)
(p/m)
2,000-40,000,000
> 17,000

l 01357 522 961

500-10,000,000

> 35,000

200-2,000,000

> 50,000

200-400,000

> 50,000

up to 30,000

> 70,000

Part No.
PL1510-5200
PL1510-1200
PL1510-5100
PL1510-1100
PL1510-5500
PL1510-1500
PL1510-5504
PL1510-1504
PL1510-5300
PL1510-1300

GPC/SEC Columns and Calibrants

PLgel Individual Pore Size Columns
• Very high efficiency improves productivity
• Choose the optimum column for a perfect match of performance and application
• Fast analysis with fewer columns saves time and money
Individual pore size GPC columns offer high resolution over a specific molecular weight range.
The linear portion of the calibration curve, where the slope is at its shallowest, defines the MW
region over which optimum resolution will be achieved.

PLgel Individual Pore Size Columns
Size (mm)
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300

Calibration curves

Linear MW
Operating Range
(g/mol) (PS)
up to 4,000
up to 2,000
up to 4,000
500-30,000
500-60,000
10,000-600,000
60,000-2,000,000
up to 2,000
500-30,000
500-30,000
500-60,000
500-60,000
10,000-600,000
60,000-2,000,000

Pore Size
(Å)
100
50
100
500
103
104
105
50
100
500
103
104
105
106

Guaranteed
Efficiency
(p/m)
> 100,000
> 60,000
> 60,000
> 60,000
> 50,000
> 50,000
> 50,000
> 35,000
> 35,000
> 35,000
> 35,000
> 35,000
> 35,000
> 35,000

Part No.
PL1110-6320
PL1110-6515
PL1110-6520
PL1110-6525
PL1110-6530
PL1110-6540
PL1110-6550
PL1110-6115
PL1110-6120
PL1110-6125
PL1110-6130
PL1110-6140
PL1110-6150
PL1110-6160

10 M

Polystyrene
THF
1.0 mL/min
106 Å

Polystyrene Molecular Weight

Calibrant:
Mobile Phase:
Flow Rate:

Particle
Size
(µm)
3
5
5
5
5
5
5
10
10
10
10
10
10
10

105 Å
104

Å

103 Å
500 Å
100 Å
50 Å

1K
4

Elution Volume (mL)

10
VLC0016

LC AND LC/MS COLUMNS

955

GPC/SEC Columns and Calibrants

PLgel Preparative Columns
• Excellent column efficiency provides optimum resolution
• High loading can isolate mg amounts for further study
• Over 10 times scale up permits efficient quantification
Preparative GPC is generally employed to fractionate polymers, isolate components in a polymer
formulation or simplify mixtures of relatively small molecules in complex matrices. Mixtures of
materials are easily separated on the basis of size, preferably in a low boiling organic solvent.
They are then collected as a series of discrete fractions and isolated by simple evaporation
of the solvent.
PLgel preparative columns are packed with the same rigid, high performance media as the
analytical columns. The 10 µm particle provides high column efficiency (> 25,000 p/m) for
optimum resolution and loading characteristics. PLgel 25 mm ID preparative columns offer over
10 times scale-up compared to the 7.5 mm analytical columns. The increased ID and column
volume permit even higher loading. With low molecular weight materials, sample concentration
can also be significantly increased, enabling production of milligram quantities of very pure
material. The actual loading is ultimately controlled by the sample and its molecular weight.

PLgel Preparative Columns
Size (mm)
25 x 300
25 x 300
25 x 300
25 x 300
25 x 300
25 x 300
25 x 300
MIXED-B
25 x 300
MIXED-D
25 x 300
Prep guard
25 x 25

956

LC AND LC/MS

Particle Size (µm)
10
10
10
10
10
10
10
10

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Pore Size
(Å)
50
10
500
103
104
105
106

10

Linear MW
Operating Range
(g/mol) (PS)
up to 2,000
up to 4,000
500-30,000
500-60,000
10,000-600,000
60,000-2,000,000
600,000-10,000,000
500-10,000,000

Part No.
PL1210-6115
PL1210-6120
PL1210-6125
PL1210-6130
PL1210-6140
PL1210-6150
PL1210-6160
PL1210-6100

200-400,000

PL1210-6104
PL1210-1120

l 01357 522 961

GPC/SEC Columns and Calibrants

Columns for Special
GPC/SEC Applications
EnviroPrep
• High sample loading ensures effective trace analysis
• Simple clean-up procedure saves sample preparation costs
• Optimized particle size distribution provides high resolution
EnviroPrep columns permit a simple, one stage clean-up as part of a methodology to determine
pesticides in many organic matrices. The higher molecular weight fractions such as lipids,
polymers, natural resins and dispersed high molecular weight components are easily eliminated
in the GPC analysis.
Preparative GPC for soil extract clean-up is described in EPA Method 3640A using 300 x 25 mm
and 150 x 25 mm columns to give higher sample loading and fraction yields, which is particularly
useful for low levels of pollutants. Low pore size EnviroPrep columns are ideal for this method.
The columns have 10 µm particles with 100Å pore sizes for high resolution, with an exclusion
limit of 4000 MW. The preparative columns offer good resolution and high loading through
optimization of the particle size distribution.

EnviroPrep
Size (mm)
21.2 x 150
25 x 150
21.2 x 300
25 x 300

Part No.
PL1E10-3120EPA
PL1210-3120EPA
PL1E10-6120EPA
PL1210-6120EPA

Columns for sample clean-up
Column:
Column:
Mobile Phase:
Flow Rate:
Detector:

1

EnviroPrep
PL1210-6120EPA
25 x 300
PL1210-3120EPA
25 x 150

3

DCM
10 mL/min
UV, 254 nm

1.
2.
3.
4.
5.

Corn oil, 25,000 mg/L
Bis(2-ethylhexyl) phthalate, 1,000 mg/L
Methoxychlor, 200 mg/L
Perylene, 20 mg/L
Sulfur, 80 mg/L

2
4

0

min

5

22
VLC0017

LC AND LC/MS COLUMNS

957

GPC/SEC Columns and Calibrants

PLgel Olexis
• Optimized design for polyolefin analysis
• High temperature capability
• High resolution with no damage from sample shear provides clean separations
PLgel Olexis is designed for the analysis of very high molecular weight polymers, specifically
polyolefins. The column resolves up to 100,000,000 g/mol (polystyrene in THF), and is packed
with 13 µm particles to optimize efficiency and resolution without the risk of sample shear
degradation during analysis. The packing of PLgel Olexis has the mechanical stability and
robustness expected from a PLgel column, and so it is able to operate up to 220°C for the
analysis of highly crystalline materials.

PLgel Olexis
Description
PLgel Olexis
PLgel Olexis guard

Column:

3 x PLgel Olexis, 7.5 x 300 mm
PL1110-6400

Mobile Phase:
Flow Rate:
Injection Volume:
Temperature:
Detector:

Trichlorobenzene + 0.0125% BHT
1.0 mL/min
200 µL
160°C
PL-GPC 220 (RI)

Part No.
PL1110-6400
PL1110-1400

1

dw/dlog M

PLgel Olexis reveals true modalities
across the range of polyolefins

Size (mm)
7.5 x 300
7.5 x 50

0
3

Log M

7
VLC0018

958

LC AND LC/MS

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l 01357 522 961

GPC/SEC Columns and Calibrants

PL HFIPgel
• Optimized separation range delivers high performance with no artifacts
• Highly durable packing prolongs column lifetime
• Low operating pressure reduces system wear and unnecessary downtimes
Hexafluoroisopropanol (HFIP) is used as a solvent in GPC for the analysis of important industrial
polymers such as polyesters, polyamides and polylactide/glycolide copolymers. For greatly
improved performance in extremely polar solvents such as HFIP and trifluoroethanol, we have
developed novel "multipore" technology to produce PL HFIPgel, a PS/DVB packing featuring
a monodisperse particle size, high pore volume and high resolution.
Using PL HFIPgel avoids issues associated with conventional packing and HFIP, such as excessive
curvature of calibration curves, dislocations/shoulders on peaks for polydisperse samples and
poor resolution in the low MW region.
Column efficiency is guaranteed > 30,000 p/m and the columns are very durable, with a
maximum operating pressure of 145 bar (2030 psi). They are packed and tested in methanol
but shipped ready-to-use in HFIP.
PL HFIPgel columns with 7.5 mm ID normally operate at 1 mL/min. However, the 4.6 mm ID
columns run at 0.3 mL/min, providing a 70% reduction in solvent consumption with consequent
savings in the cost of buying and disposing of solvents.

LC AND LC/MS COLUMNS

959

GPC/SEC Columns and Calibrants

PL HFIPgel
Description
PL HFIPgel
PL HFIPgel
PL HFIPgel guard
PL HFIPgel guard

Size (mm)
4.6 x 250
7.5 x 300
7.5 x 50
4.6 x 50

Part No.
PL1514-5900HFIP
PL1114-6900HFIP
PL1114-1900HFIP
PL1514-1900HFIP

Polyamides
Column:

2 x PL HFIPgel, 7.5 x 300 mm
PL1110-6400

Mobile Phase:
Flow Rate:
Temperature:
Detector:

HFIP + 20mM NaTFAc
1.0 mL/min
40°C
PL-GPC 50 Plus (RI)

Nylon 66

Nylon 6

PBT

5

min

22
VLC0019

960

LC AND LC/MS

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l 01357 522 961

GPC/SEC Columns and Calibrants

PL Rapide
• Analysis in less than ten minutes saves time
• Significantly increased sample throughput improves efficiency
• Reduced solvent consumption and disposal costs save money
• Available in L, M and H versions for low, medium and high molecular weights;
available in F version for flow injection analysis

Rapid GPC is an excellent tool for screening polymer MWD for trend analysis. Short PL Rapide
columns reduce analysis times while maintaining the excellent solvent compatibility and
mechanical stability of all GPC columns from Agilent.
PL Rapide columns are ideal for high speed applications such as high throughput screening,
process monitoring, or tracking changes in MW distributions, where time is the most critical
factor in the analysis. Packed with high quality gels, these columns cover the complete
spectrum of molecular weights and are available for the analysis of both organic and water
soluble polymers. Key features include high pore volume and high resolution packing materials,
no special system requirements, choice of molecular weight resolving range, wide solvent
compatibility, and excellent mechanical stability.

Tips & Tools
Agilent offers a variety of e-Seminars and on-site training
to help you learn how to be a more effective chromatographer.
For more information, visit www.agilent.com/chem/education

LC AND LC/MS COLUMNS

961

GPC/SEC Columns and Calibrants

PL Rapide
MW Range
Guaranteed
Size (mm)
(g/mol)
Efficiency (p/m)
7.5 x 150 500-10,000,000
> 35,000
10 x 100

Description
PL Rapide H

Part No.
PL1113-3100
PL1013-2100

PL Rapide M

7.5 x 150
10 x 100

200-2,000,000

> 60,000

PL1113-3500
PL1013-2500

PL Rapide L

7.5 x 150
10 x 100

200-400,000

> 80,000

PL1113-3300
PL1013-2300

PL Rapide F

7.5 x 150
10 x 100

up to 4,500
up to 4,000

> 55,000
> 40,000

PL1113-3120
PL1013-2120

PL Rapide Aqua H

7.5 x 150
10 x 100

100-10,000,000

> 35,000

PL1149-3800
PL1049-2800

PL Rapide Aqua L

7.5 x 150
10 x 100

100-30,000

> 35,000

PL1120-3830
PL1020-2830

Resin analysis by rapid GPC
Column:

PL Rapide L
PL1013-2300
10 x 100 mm

Sample:
Mobile Phase:
Flow Rate:
Detector:

Epoxy resin
THF
1.0 , 2.0 and 3.0 mL/min
UV, 254 nm

1.0 mL/min

2.0 mL/min

3.0 mL/min
0

min

6
VLC0020

962

LC AND LC/MS

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l 01357 522 961

GPC/SEC Columns and Calibrants

PolarGel
• Medium polarity surface and high mechanical stability
• Operate in a wide range of solvents and solvent combinations
• Available in two resolving ranges, PolarGel-L and PolarGel-M
The PolarGel range is ideal for use with polar solvents, such as dimethyl formamide (DMF)
and dimethyl sulfoxide (DMSO), and for solvent combinations such as tetrahydrofuran with
water. These eluents are very useful in GPC/SEC to separate polar materials, such as polar
resins, modified polysaccharides or complex polar polymers that are difficult to analyze in
traditional SEC solvents, such as tetrahydrofuran alone. PolarGel-L is used for low molecular
weight polar polymers and PolarGel-M for high MW polar polymers.
With polar polymers, highly polar groups can lead to non-specific interactions and secondary
separation mechanisms when using polar solvents and traditional non-polar
styrene/divinylbenzene columns. Additives and/or column conditioning are normally required to
reduce these interactions. PolarGel has no need for these interventions, and also avoids the
interactions and secondary effects that produce chromatogram distortions.
These PolarGel "mixed bed" columns have a medium polarity surface and high mechanical
stability. They are capable of operating in a wide range of solvents and solvent combinations,
greatly enhancing your ability to analyze polar polymers that are not necessarily water soluble.
PolarGel is available in two resolving ranges to meet your precise requirements.

PolarGel
Description
PolarGel-L
PolarGel-L guard
PolarGel-L repair gel
PolarGel-M
PolarGel-M guard
PolarGel-M repair gel

Size (mm)
7.5 x 300
7.5 x 50
7.5 x 300
7.5 x 50

Part No.
PL1117-6830
PL1117-1830
PL1417-0830
PL1117-6800
PL1117-1800
PL1417-0800

LC AND LC/MS COLUMNS

963

GPC/SEC Columns and Calibrants

Two samples of melamine resin
analyzed by PolarGel-L
Column:

2 x PolarGel-L, 300 x 7.5 mm
PL1117-6830

Mobile Phase:
Flow Rate:
Injection Volume:
Detector:

Dimethylacetamide + 0.1% LiBr
1.0 mL/min
100 µL
Agilent PL-GPC 220 (RI)

0

min

22
VLC0021

Excellent separation of two phenol
formaldehyde resins with PolarGel-M
Column:

2 x PolarGel-M, 300 x 7.5 mm
PL1117-6800

Mobile Phase:

0.2% (w/v) DMF & 0.1% LiBr to reduce
sample aggregation
Flow Rate:
1.0 mL/min
Injection Volume: 100 µL
Detector:
Agilent PL-GPC 50 (RI)

0

min

22
VLC0022

964

LC AND LC/MS

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www.crawfordscientific.com

l 01357 522 961

GPC/SEC Columns and Calibrants

PlusPore
The PlusPore range has an increased pore volume that provides high resolution for specific
applications. The high stability media permits the use of a wide range of organic solvents
with accuracy and precision so that there is no distortion of the MW distribution shape.
The PlusPore series of columns has been specifically designed for high resolution GPC,
and represents the very latest in GPC column technology. These novel packing materials
are based on the industry standard, highly cross-linked polystyrene/divinylbenzene (PS/DVB),
for the widest applicability and solvent compatibility. Each is made using a novel polymerization
process to produce particles that exhibit a specific, controlled pore structure for optimum
GPC performance. Typical applications include resins, condensation polymers, prepolymers,
and oligomers.
For high resolution polymer analysis, the PolyPore, ResiPore, MesoPore and OligoPore columns
of the PlusPore product series exhibit a wide pore size distribution with near linear calibration
curves covering an extended molecular weight range. These so-called "multipore" structures
have increased pore volume compared to regular PS/DVB packing materials. This results in very
high resolution GPC columns designed for specific application areas. The highly cross-linked
porous particles provide excellent chemical and physical stability and permit easy transfer across
the full range of organic solvents with little change in the shape of the calibration curve or the
efficiency of the columns. As this multipore column technology does not require the combination
of individual pore size packing materials, the result is high accuracy and precision without any
artifacts in the shape of the molecular weight distribution.

LC AND LC/MS COLUMNS

965

GPC/SEC Columns and Calibrants

PlusPore calibration curves
Key

6.5

PolyPore
ResiPore

Log MW

MesoPore
OligoPore

2

Retention time/min

4

10
VLC0023

PlusPore Selection Guide
Column
PolyPore
ResiPore
MesoPore
OligoPore

966

MW Range
(g/mol) (PS)
200-2,000,000
200-400,000
up to 25,000
up to 4,500

LC AND LC/MS

Nominal
Particle Size (µm)
5
3
3
6

Typical Efficiency
(p/m)
> 60,000
> 80,000
> 80,000
> 55,000

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Recommended Calibrants
EasiCal PS-1or EasiVial PS-H
EasiCal PS-2 or EasiVial PS-M
Polystyrene S-L-10 Kit
Polystyrene S-L2-10 Kit

l 01357 522 961

Frit Porosity (µm)
2
2
2
2

GPC/SEC Columns and Calibrants

PolyPore
• Routine polymer analysis with very high resolution
• Wide operating range simplifies column choice
• Low particle size extracts maximum information from the analyte
PolyPore columns have been specifically developed to give unrivaled resolution for the analysis of
polymers with broad molecular weight distributions. With a wide operating range covering many
decades of molecular weight, PolyPore columns combine a low 5 µm particle size with extremely
high pore volume to give the highest possible resolution, ensuring the most detailed information
possible from your analysis.

PolyPore
Description
PolyPore
PolyPore guard

Size (mm)
7.5 x 300
7.5 x 50

Part No.
PL1113-6500
PL1113-1500

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

LC AND LC/MS COLUMNS

967

GPC/SEC Columns and Calibrants

Comparison of PolyPore with conventional
individual pore size GPC columns
Column:

2 x PolyPore, 300 x 7.5 mm
PL1113-6500

Sample:
Mobile Phase:
Flow Rate:
Injection Volume:
Detector:

High MW Resin
THF
1.0 mL/min
100 µL
Agilent PL-GPC 50 (RI)

Individual Pore Size Columns

PolyPore

5

Retention time/min

20
VLC0024

Polymethylmethacrylate in DMF
Column:

2 x PolyPore, 7.5 x 300 mm
PL1113-6500

Sample:
Mobile Phase:
Flow Rate:
Temperature:
Injection Volume:
Detector:

Commercial PMMA
DMF + 0.1% LiBr
1.0 mL/min
80°C
100 µL
Agilent PL-GPC 50 (RI)

0

Retention time/min

24
VLC0025

968

LC AND LC/MS

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l 01357 522 961

GPC/SEC Columns and Calibrants

ResiPore
• Efficient separation of complex molecular weight distributions
• Reveals oligomer content to provide a true representation of the sample
• High pore volume extracts maximum information from the analyte
ResiPore columns are the ideal choice for the analysis of resins and condensation polymers with
complex molecular weight distributions that include oligomer content. By combining a low 3 µm
particle size and high pore volume, high efficiency ResiPore columns offer maximum resolution
of these intermediate molecular weight polymers.

ResiPore
Description
ResiPore
ResiPore guard

Size (mm)
7.5 x 300
7.5 x 50

Part No.
PL1113-6300
PL1113-1300

LC AND LC/MS COLUMNS

969

GPC/SEC Columns and Calibrants

Alkyd resin
Column:

2 x ResiPore, 7.5 x 300 mm
PL1113-6500

Mobile Phase:
Flow Rate:
Injection Volume:
Detector:

THF
1.0 mL/min
20 µL
UV, 254 nm

5

Retention time/min

20
VLC0026

Polyester
Column:

2 x ResiPore, 7.5 x 300 mm
PL1113-6500

Mobile Phase:
Flow Rate:
Injection Volume:
Detector:

THF
1.0 mL/min
20 µL
UV, 254 nm

5

Retention time/min

20
VLC0027

970

LC AND LC/MS

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l 01357 522 961

GPC/SEC Columns and Calibrants

MesoPore
• Full solvent compatibility with no detrimental effect on efficiency
• Low particle size extracts maximum information from the analyte
• No MWD dislocations so the distribution is an accurate representation of the sample
MesoPore columns have been specifically designed to provide optimum results in the analysis of
prepolymers, i.e. polymeric materials with a large oligomeric component. By combining a 3 µm
particle size with high pore volume, MesoPore columns give the highest resolution separations
for the analysis of low molecular weight polymers, such as prepolymers, resins, polyols and
siloxanes.

MesoPore
Description
MesoPore
MesoPore guard

Size (mm)
7.5 x 300
7.5 x 50

Part No.
PL1113-6325
PL1113-1325

Polyurethanes
Column:

2 x MesoPore, 7.5 x 300 mm
PL1113-6500

Mobile Phase:
Flow Rate:
Injection Volume:
Detector:

THF
1.0 mL/min
20 µL
Agilent PL-GPC 50 (RI)

5

Retention time/min

22
VLC0028

Polyesterimide
Column:

2 x MesoPore, 7.5 x 300 mm
PL1113-6500

Mobile Phase:
Flow Rate:
Injection Volume:
Detector:

THF
1.0 mL/min
20 µL
Agilent PL-GPC 50 (RI)

5

Retention time/min

17
VLC0029

LC AND LC/MS COLUMNS

971

GPC/SEC Columns and Calibrants

OligoPore
• Near linear calibration curve for best accuracy and precision
• Very stable media allows for a wide choice of solvents
• Isolation of individual fractions reveals more information from whole samples
OligoPore columns have been developed from an innovative new media that exhibits significantly
increased pore volumes compared to conventional low pore size GPC columns. The outcome is
higher resolution in the oligomeric region. The 300 x 25 mm preparative column offers high
resolution at greatly increased loading for effective isolation of individual components. Oligomer
fractions collected from the OligoPore preparative column can then be re-injected on analytical
columns to check for the purity of the fractions and for comparison with the whole sample.

OligoPore
Description
OligoPore
OligoPore
OligoPore guard

Size (mm)
25 x 300
7.5 x 300
7.5 x 50

Tips & Tools
Don't forget, we have special offers throughout the year.
To learn more, visit www.agilent.com/chem/specialoffers

972

LC AND LC/MS

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l 01357 522 961

Part No.
PL1213-6520
PL1113-6520
PL1113-1320

GPC/SEC Columns and Calibrants

Analysis of low molecular weight
polystyrene and oligomer fractions collected
from OligoPore preparative columns
Column:

2 x OligoPore, 7.5 x 300 mm
PL1113-6500

Mobile Phase:
Flow Rate:
Detector:

THF
1.0 mL/min
UV

Whole polymer
F9
F8
F7
F6
F5
F4
F3
F2
F1
10

Retention time/min

20
VLC0030

Analytical separation of
low molecular weight polystyrene
Column:

2 x OligoPore, 7.5 x 300 mm
PL1113-6500

Mobile Phase:
Flow Rate:
Loading:
Detector:

THF
1.0 mL/min
0.2%, 100 mL
UV

5

Retention time/min

5

Retention time/min

20

Preparative separation of
low molecular weight polystyrene
Column:

2 x OligoPore, 25 x 300 mm
PL1113-6500

Mobile Phase:
Flow Rate:
Loading:
Detector:

THF
10.0 mL/min
2.0%, 2 mL
UV

20
VLC0031

LC AND LC/MS COLUMNS

973

GPC/SEC Columns and Calibrants

Aqueous SEC of Polymers
PL aquagel-OH SEC
Aqueous size exclusion chromatography (SEC) is widely used for the determination of
molecular weight distributions of a variety of synthetic and naturally occurring water-soluble
polymers, and separations of oligomers and small molecules. The requirement to eliminate ionic
and hydrophobic effects makes aqueous SEC very demanding.
The PL aquagel-OH series provides a chemically and physically stable matrix for reliable aqueous
SEC separations. The columns are packed with macroporous copolymer beads with an extremely
hydrophilic polyhydroxyl functionality. The "neutral" surface and the capability to operate across a
wide range of eluent conditions provide for high performance analyses of compounds with
neutral, ionic and hydrophobic moieties, alone or in combination. PL aquagel-OH is available for
analytical and preparative applications.

Optimizing Conditions for Aqueous SEC
with PL aquagel-OH Columns
Due to the complex nature of water-soluble polymers, it is often necessary to modify the eluent
in order to avoid sample-to-sample and sample-to-column interactions which can result in poor
aqueous SEC separations. The excellent stability of the PL aquagel-OH packing material allows
the eluent to be tailored to suit the polymer, while retaining the high column efficiency. For ionic
interactions, the eluent can be modified by the addition of salt and/or the adjustment of pH.
For water soluble polymers with a hydrophobic character, only the addition of a weak organic
solvent (methanol) is required to inhibit hydrophobic interactions.

974

LC AND LC/MS

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l 01357 522 961

GPC/SEC Columns and Calibrants

Guide to eluent selection for PL aquagel-OH applications
Water soluble polymer

Polymer Chemistry

Suggested Eluent

Hydrophobic Polymers

Neutral

Anionic

Cationic

Pure Water

0.1-0.3 M NaNO3
pH 7-9
0.01 M NaH2P04

0.2-0.8 M NaNO3
pH 2-7
0.01 M NaH2P04

Addition of up to 50% methanol

PL aquagel-OH Column Selection Guide
Sample Type
Low MW polymers and oligomers

Typical Applications
Surfactants, oligosaccharides, PEGs,
lignosulfonates, polyacrylates

Polydisperse synthetic or naturally occurring polymers Polysaccharides, PVA, cellulose derivatives,
PEO, polyacrylic acid
Very high MW polymers

Polyacrylamides, hyaluronic acids, CMC,
starches, gums

Recommended Column Sets
2 or 3 30, 20
PL aquagel-OH 8 µm, or
PL aquagel-OH 20 5 µm, or
PL aquagel-OH MIXED-M 8 µm
2 or 3
PL aquagel-OH MIXED-H 8 µm, or
PL aquagel-OH 60/50/40 8 µm
PL aquagel-OH 60/50/40 15 µm in series

LC AND LC/MS COLUMNS

975

GPC/SEC Columns and Calibrants

PL aquagel-OH Analytical

Tips & Tools
Buffers in a stored column may
crystallize and cause damage.
Flush the column with water
containing a small amount
of sodium azide to prevent
biological growth.

• Highly stable matrix ensures reliable separations, even with modified eluents
• MIXED columns cover a wide range of molecular weights, simplifying column selection
• Highly versatile for neutral, polar, anionic and cationic samples
The PL aquagel-OH analytical series has a pH range of 2-10, compatibility with organic
solvent (up to 50% methanol), mechanical stability up to 140 bar (2030 psi) and low column
operating pressures.

PL aquagel-OH Analytical
Description
PL aquagel-OH 20 5 µm
PL aquagel-OH 20 8 µm
PL aquagel-OH 30 8 µm
PL aquagel-OH 40 8 µm
PL aquagel-OH 40 15 µm
PL aquagel-OH 50 8 µm
PL aquagel-OH 50 15 µm
PL aquagel-OH 60 8 µm
PL aquagel-OH 60 15 µm
PL aquagel-OH
MIXED-H 8 µm
PL aquagel-OH
MIXED-M 8 µm
PL aquagel-OH
10 µm guard
PL aquagel-OH
5 µm guard
PL aquagel-OH
8 µm guard

976

LC AND LC/MS

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Size (mm)
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300
7.5 x 300

MW Range
(g/mol) (PEG/PEO)
100-20,000
100-20,000
100-30,000
10,000-200,000
10,000-200,000
50,000-1,000,000
50,000-1,000,000
200,000 - > 10,000,000
200,000 - > 10,000,000
100-10,000,000

Guaranteed
Efficiency
(p/m)
> 55,000
> 35,000
> 35,000
> 35,000
> 15,000
> 35,000
> 15,000
> 35,000
> 15,000
> 35,000

Part No.
PL1120-6520
PL1149-6820
PL1120-6830
PL1149-6840
PL1149-6240
PL1149-6850
PL1149-6250
PL1149-6860
PL1149-6260
PL1149-6800

100-10,000,000

> 35,000

PL1149-6801

25 x 25

PL1249-1120

7.5 x 50

PL1149-1530

7.5 x 50

PL1149-1840

l 01357 522 961

GPC/SEC Columns and Calibrants

Polyvinyl alcohol
Column:

3 x PL aquagel-OH MIXED
PL1149-6800
7.5 x 300 mm, 8 µm

Mobile Phase:
Flow Rate:
Detector:

0.2 M NaNO3, 0.01 M NaH2PO4, pH 7
1.0 mL/min
Agilent PL-GPC 50 (RI)

10

Retention time/min

33
VLC0032

Heparin
Column:

2 x PL aquagel-OH 30
PL1120-6830
7.5 x 300 mm, 8 µm

Mobile Phase:
Flow Rate:
Detector:

0.2 M NaNO3, 0.01 M NaH2PO4, pH 7
1.0 mL/min
Agilent PL-GPC 50 (RI)

5

Retention time/min

22
VLC0033

LC AND LC/MS COLUMNS

977

GPC/SEC Columns and Calibrants

Hyaluronic acid
Column:
Column:

Mobile Phase:
Flow Rate:
Detector:

PL aquagel-OH 60 15 µm
PL1149-6260
7.5 x 300 mm, 15 µm
PL aquagel-OH 40 15 µm
PL1149-6240
7.5 x 300 mm, 15 µm

Chromatogram

0.2 M NaNO3, 0.01 M NaH2PO4, pH 7
1.0 mL/min
Agilent PL-GPC 50 (RI)

5

min

18

dw/dlog M

1

Mw 1,800,00 g/mol

0
5

Log M

7
VLC0034

2 x PL aquagel-OH 20
PL1120-6520
7.5 x 300 mm, 5 µm

Mobile Phase:
Flow Rate:
Injection Volume:
Software:
Detector:

0.25 M ammonium formate in water
1.0 mL/min
20 µL
Cirrus GPC module for Galaxie CDS
ELS (neb=30°C, evap=30°C, gas=1.4
SLM)

1.0

Cum.Ht

Column:

8.0

dw/Log M

Differences in composition of two alkyl
naphthalene sulfonates

0.0

0.0
1.0

Log M

3.5
VLC0035

978

LC AND LC/MS

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l 01357 522 961

GPC/SEC Columns and Calibrants

PL aquagel-OH Preparative
• Up to 10 times scale-up maximizes yield
• High loading maximizes sample throughput
• Carefully chosen particle size provides optimum resolution
Preparative SEC is used for the fractionation of a wide variety of water-soluble samples based
on their size in solution. The technique is applied to the fractionation of disperse polymers or
to isolate components in a polymer formulation.
Preparative PL aquagel-OH columns and associated guard columns enable rapid and convenient
scale-up from analytical separations. The 25 mm ID prep column offers at least a 10 times
scale-up in loading from the 7.5 mm ID analytical columns. Typically, a 10 mL/min flow rate
results in a separation time of ten minutes with a 300 mm column. The columns are packed
with the same robust macroporous particles as the analytical column range. The 8 µm
article size provides optimum resolution and loading characteristics with column efficiency
> 20,000 plates/m.

PL aquagel-OH Preparative
Description
PL aquagel-OH 30 8 µm
PL aquagel-OH 40 8 µm
PL aquagel-OH 50 8 µm
PL aquagel-OH MIXED 8 µm
PL aquagel-OH 10 µm guard

Size (mm)
25 x 300
25 x 300
25 x 300
25 x 300
25 x 25

MW Range
(g/mol) (PEG/PEO)
100-30,000
10,000-200,000
50,000-1,000,000
100-10,000,000

Part No.
PL1220-6130
PL1249-6140
PL1249-6150
PL1249-6100
PL1249-1120

LC AND LC/MS COLUMNS

979

GPC/SEC Columns and Calibrants

Polyvinyl alcohol
Column:

PL aquagel-OH 40 8 µm
PL1249-6140
25 x 300 mm, 8 µm

Mobile Phase:
Flow Rate:
Loading:
Detector:

0.2 M NaNO3, 0.01 M NaH2PO4, pH 7
10.0 mL/min
10 mg/mL, 2 mL
Agilent PL-GPC 50 (RI)

Retention time/min

0

15
VLC0036

GPC Column Accessories
Description
Frit removal tool for threaded columns only
2 µm frit kit for threaded columns, 7.5 mm ID
5 µm frit kit for threaded columns, 7.5 mm ID
10 µm frit kit for threaded columns, 7.5 mm ID
PLgel column repair gel, 10 µm
PLgel column repair gel, 5 µm
Column connecting nuts, 1/16 in. tube
Tubing ferrules, 1/16 in. tube
Connecting tubing, 10 cm length, 0.01 in. ID
LDV intercolumn stainless steel connector

980

LC AND LC/MS

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l 01357 522 961

Unit
1/pk
5/pk
5/pk
5/pk
1/pk
1/pk
5/pk
5/pk
10/pk
1/pk

Part No.
PL1310-0001
PL1310-0002
PL1310-0012
PL1310-0036
PL1410-0101
PL1410-0501
PL1310-0007
PL1310-0008
PL1310-0048
PL1310-0005

GPC/SEC Columns and Calibrants

Polymer Standards for GPC/SEC
Polymer standards from Agilent are the ideal reference materials for generating accurate, reliable
GPC/SEC column calibrations, with the assurance of the ISO 9001:2000 quality standard.
Additional applications for our highly characterized homopolymers and copolymers exhibiting
unique characteristics are as model polymers for research and analytical method development.
Agilent manufactures the highest quality polymer standards with extremely narrow polydispersity
and the widest molecular weight range commercially available. These quality polymer standards
are supplied with extensive characterization data utilizing a variety of independent techniques
(e.g. light scattering and viscometry) and high performance GPC to verify polydispersity and
assign that all important peak molecular weight (Mp).
Our comprehensive range of EasiVial, EasiCal and traditional calibration kits has been specifically
designed to cover all molecular weight ranges for organic and aqueous GPC/SEC applications.
We provide you with the widest choice to maximize your specific characterization needs. In
addition, we supply other polymers as individual molecular weights, and broad distribution
polymers for system validation or broad standard calibration procedures.

LC AND LC/MS COLUMNS

981

GPC/SEC Columns and Calibrants

Calibration Kits
Agilent offers a wide range of polymer standards kits for conventional GPC/SEC column
calibration or for calibrating light scattering and viscometry detectors. The kits are in boxed sets
of ten different polymer standards covering a particular molecular weight range, to be used with
organic and aqueous, medium polarity and polar solvents. Every individual polymer has its own
Certificate of Analysis of the analytical conditions and values, such as Mp needed for
constructing a calibration plot. The polymers are chosen to give equidistant calibration points on
a logarithmic MW scale, providing a more uniform calibration curve.

Individual Polymer Molecular Weights
We design our individual standards to have the narrowest molecular weight distribution
commercially available. Additionally, they cover the widest molecular weight range, from
162-15 million MW. The current polystyrene nominal molecular weight of 15 million MW
has a polydispersity ≤1.10. These standards are generally available in 1, 5 and 10 g
quantities, and each comes with its own Certificate of Analysis detailing analysis conditions
and relevant data.

GPC/SEC Standards Selection Guide
Polymer Type
Polystyrene
Polymethylmethacrylate
Polyethylene
Polyethylene glycol (PEG)
Polyethylene oxide (PEO)
Pullulan polysaccharide
Polyacrylic acid Na salt

982

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Individual Calibration
MW
Kits
EasiCal EasiVial Type of GPC/SEC
]
]
]
]
Organic
]
]
]
Organic
]
]
Organic
]
]
]
Organic/Aqueous
]
]
]
Organic/Aqueous
]
]
Organic/Aqueous
]
]
Aqueous

l 01357 522 961

GPC/SEC Columns and Calibrants

EasiVial
• Eliminates tedious weighing procedures to improve calibration accuracy
• Reduces solvent dispensing to limit risks associated with handling solvents
• For conventional and multi-detector GPC to maximize applicability
For organic and aqueous GPC/SEC column calibration, this premier product is the quickest
and most convenient method to deliver an accurate 12-point column calibration.
The key to achieving baseline separation from polymer mixtures, therefore eliminating doubt
and errors, is in selecting only the narrowest polydispersity polymers. This is where Agilent
polymer standards excel and deliver, as shown in the chromatograms.
The EasiVial standards kit is a pre-prepared, time saving product for rapid and reliable GPC
column calibration. EasiVial kits contain three vials, each with a mixture of four accurately
pre-weighed polymer standards, providing a 12-point GPC calibration in just three injections.
The mass of each polymer in the vial is accurately known, so that upon addition of a fixed
volume of eluent, the solution is prepared at a precise concentration. EasiVial is ideal for both
conventional and multi-detector GPC calibration. Simply prepare and manually inject, or transfer
to autosampler vials, or place directly into a compatible autosampler.
Every EasiVial kit contains 30 vials (ten of each type) that are color-coded for easy identification
and are available in 4 or 2 mL vials making them suitable for most autosamplers. The kits are
available for polystyrene (PS), polymethylmethacrylate (PMMA), polyethylene glycol/oxide
(PEG/PEO) and polyethylene glycol (PEG). For added value, a Tri-Pack (90 vials) is offered,
extending reproducibility.

Polystyrene calibration generated with EasiVials

LogM

1.0e7

1.0e2
15

Retention time/min

29
VLC0044

LC AND LC/MS COLUMNS

983

GPC/SEC Columns and Calibrants

EasiVial Pre-weighed Calibration Kits
Range of Nominal Mp
(g/mol)
100-1,200,000
100-1,200,000
106-35,000
106-35,000
600-2,000,000
600-2,000,000
162-6,000,000
162-6,000,000
162-400,000
162-400,000
162-40,000
162-40,000

Description
EasiVial PEG/PEO
EasiVial PEG/PEO
EasiVial PEG
EasiVial PEG
EasiVial PM
EasiVial PM
EasiVial PS-H
EasiVial PS-H
EasiVial PS-M
EasiVial PS-M
EasiVial PS-L
EasiVial PS-L
PEG/PEO Tri-Pack
PEG/PEO Tri-Pack
PEG Tri-Pack
PEG Tri-Pack
PMMA Tri-Pack
PMMA Tri-Pack
PS-H Tri-Pack
PS-H Tri-Pack
PS-L Tri-Pack
PS-L Tri-Pack

Vial Volume
(mL)
2
4
2
4
2
4
2
4
2
4
2
4
2
4
2
4
2
4
2
4
3
4

Unit
30/pk
30/pk
30/pk
30/pk
30/pk
30/pk
30/pk
30/pk
30/pk
30/pk
30/pk
30/pk
90/pk
90/pk
90/pk
90/pk
90/pk
90/pk
90/pk
90/pk
90/pk
90/pk

Part No.
PL2080-0201
PL2080-0200
PL2070-0201
PL2070-0200
PL2020-0201
PL2020-0200
PL2010-0201
PL2010-0200
PL2010-0301
PL2010-0300
PL2010-0401
PL2010-0400
PL2080-0202
PL2080-0203
PL2070-0202
PL2070-0203
PL2020-0202
PL2020-0203
PL2010-0202
PL2010-0203
PL2010-0402
PL2010-0403

EasiVial PS-H
Column:

3 x PLgel MIXED-B, 10 µm
PL1110-6100
7.5 x 300 mm, 10 µm

Mobile Phase:
Flow Rate:
Temperature:
Detector:

THF
1.0 mL/min
40°C
PL-GPC 220 (RI)

1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

4

3
2
1

7

6

8

5

10

11

12

9

10

min

33
VLC0037

984

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

6,035,000
483,000
19,720
1,260
3,053,000
84,900
8,450
580
915,000
60,450
3,370
162

GPC/SEC Columns and Calibrants

EasiCal
• Easy three-step process with no fuss
• Cost-effective format saves money
• Only two injections for improved productivity
The EasiCal system for organic solvents consists of two different combs, each with ten
detachable spatulas, supporting a mixture of five polymer standards. The thin film of polymer
(approximately 5 mg) on the tip of the PTFE spatulas rapidly dissolves when immersed in eluent
to provide two GPC/SEC calibration solutions. A single pack provides ten spatulas of each type,
with MWs selected to provide equidistant calibration points for greater accuracy.

EasiCal Pre-prepared Polystyrene Kits
Description
Polystyrene PS-1

Range of Nominal Mp (g/mol)
580-7,500,000

Polystyrene PS-2

580-400,000

Unit
1/pk
5/pk
1/pk
5/pk

Part No.
PL2010-0501
PL2010-0505
PL2010-0601
PL2010-0605

Column calibration for GPC/SEC is as easy as 1, 2, 3...

Log MW

7

1. Place one spatula of each type into appropriate volume of solvent.
3
13

Retention time/min

25
VLC0038

3. Generate a 10-point calibration
5

Retention time/min

30

5

Retention time/min

30

2. Chromatograph each solution; only two injections required

LC AND LC/MS COLUMNS

985

GPC/SEC Columns and Calibrants

Polystyrene
• Compatible with most organic solvents
• Certificate of Analysis meets international protocols
• Calibration capability for virtually all applications
Polystyrene standards are the first choice for many organic solvents, either for conventional GPC
column calibration or for calibrating light scattering and viscosity detectors. Our organic polymers
cover a range from 162-15 million MW, with MWs selected to provide equidistant calibration
points for greater accuracy. Every kit contains 0.5 g of ten different molecular weight standards.

Calibration Kits
Description
S-H-10, 10 x 0.5 g
S-H2-10, 10 x 0.5 g
S-M-10, 10 x 0.5 g
S-M2-10, 10 x 0.5 g
S-L-10, 10 x 0.5 g
S-L2-10, 10 x 0.5 g

Range of Nominal Mp (g/mol)
300,000-15,000,000
1,000-15,000,000
580-3,000,000
580-300,000
162-20,000
162-4,500

Tips & Tools
More information is a click away. We have a variety of
educational primers, application notes, maintenance guides,
and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

986

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

Part No.
PL2010-0103
PL2010-0104
PL2010-0100
PL2010-0102
PL2010-0101
PL2010-0105

GPC/SEC Columns and Calibrants

Individual Polymer Molecular Weights
Polymer Nominal Mp
(g/mol)
162
370
580
1,000
1,300
2,000
3,000
5,000
7,000
10,000
20,000
30,000
50,000
70,000
100,000
130,000
200,000
300,000
500,000
700,000
1,000,000
1,500,000
2,000,000
2,500,000
4,000,000
7,000,000
10,000,000
15,000,000

1g
Part No.
PL2012-1001
PL2012-0001
PL2012-2001
PL2012-3001
PL2012-4001
PL2012-5001
PL2012-6001
PL2012-7001
PL2012-8001
PL2012-9001
PL2013-1001
PL2013-2001
PL2013-3001
PL2013-4001
PL2013-5001
PL2013-6001
PL2013-7001
PL2013-8001
PL2013-9001
PL2014-0001
PL2014-1001
PL2014-2001
PL2014-3001
PL2014-4001
PL2014-6001
PL2014-7001
PL2014-8001
PL2014-9001

Nominal Mw/Mn
1.00
1.11
1.11
1.09
1.07
1.05
1.04
1.03
1.04
1.02
1.02
1.02
1.03
1.03
1.02
1.01
1.05
1.03
1.03
1.03
1.05
1.04
1.04
1.05
1.04
1.04
1.06
1.06

5g
Part No.
PL2012-1005
PL2012-0005
PL2012-2005
PL2012-3005
PL2012-4005
PL2012-5005
PL2012-6005
PL2012-7005
PL2012-8005
PL2012-9005
PL2013-1005
PL2013-2005
PL2013-3005
PL2013-4005
PL2013-5005
PL2013-6005
PL2013-7005
PL2013-8005
PL2013-9005
PL2014-0005
PL2014-1005
PL2014-2005
PL2014-3005
PL2014-4005
PL2014-6005
PL2014-7005
PL2014-8005
PL2014-9005

10 g
Part No.
PL2012-1010
PL2012-0010
PL2012-2010
PL2012-3010
PL2012-4010
PL2012-5010
PL2012-6010
PL2012-7010
PL2012-8010
PL2012-9010
PL2013-1010
PL2013-2010
PL2013-3010
PL2013-4010
PL2013-5010
PL2013-6010
PL2013-7010
PL2013-8010
PL2013-9010
PL2014-0010
PL2014-1010
PL2014-2010
PL2014-3010
PL2014-4010
PL2014-6010
PL2014-7010
PL2014-8010
PL2014-9010

Polystyrene standards
Column:

2 x OligoPore
PL1113-6520
7.5 x 300

Mobile Phase:
Flow Rate:
Detector:

THF
1.0 mL/min
Agilent PL-GPC 50 (RI)

1,270

580

0

min

20
VLC0039

LC AND LC/MS COLUMNS

987

GPC/SEC Columns and Calibrants

Polymethylmethacrylate
• Many solvent options increase applicability
• Stringent quality control improves performance
• Proprietary manufacturing methods ensure consistent supply
Polymethylmethacrylate (PMMA) standards are extremely versatile as they can be used for
organic GPC with a wide range of medium polarity eluents, such as tetrahydrofuran, toluene,
methyl ethyl ketone, and ethyl acetate. They also work well with more polar organic eluents, for
example dimethylformamide, dimethylacetamide, and hexafluoroisopropanol. The MWs are
selected to provide equidistant calibration points for greater accuracy, covering from 600-1.5
million MW. Every kit contains 0.5 g of ten different molecular weight standards.

Calibration Kits
Description
M-L-10, 10 x 0.5 g
M-M-10, 10 x 0.5 g

988

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Range of Nominal Mp (g/mol)
600-50,000
1,000-1,500,000

l 01357 522 961

Part No.
PL2020-0100
PL2020-0101

GPC/SEC Columns and Calibrants

Individual Polymer Molecular Weights
Polymer Nominal Mp
(g/mol)
500
1,000
2,000
3,000
5,000
7,000
10,000
13,000
20,000
30,000
50,000
70,000
100,000
130,000
200,000
300,000
500,000
700,000
1,000,000
15,000,000

Nominal
Mw/Mn
1.19
1.26
1.08
1.08
1.09
1.08
1.03
1.03
1.03
1.02
1.02
1.02
1.02
1.05
1.02
1.02
1.06
1.03
1.09
1.09

1g
Part No.
PL2022-2001
PL2022-3001
PL2022-5001
PL2022-6001
PL2022-7001
PL2022-8001
PL2022-9001
PL2023-0001
PL2023-1001
PL2023-2001
PL2023-3001
PL2023-4001
PL2023-5001
PL2023-6001
PL2023-7001
PL2023-8001
PL2023-9001
PL2024-0001
PL2024-1001
PL2024-2001

5g
Part No.
PL2022-2005
PL2022-3005
PL2022-5005
PL2022-6005
PL2022-7005
PL2022-8005
PL2022-9005
PL2023-0005
PL2023-1005
PL2023-2005
PL2023-3005
PL2023-4005
PL2023-5005
PL2023-6005
PL2023-7005
PL2023-8005
PL2023-9005
PL2024-0005
PL2024-1005
PL2024-2005

10 g
Part No.
PL2022-2010
PL2022-3010
PL2022-5010
PL2022-6010
PL2022-7010
PL2022-8010
PL2022-9010
PL2023-0010
PL2023-1010
PL2023-2010
PL2023-3010
PL2023-4010
PL2023-5010
PL2023-6010
PL2023-7010
PL2023-8010
PL2023-9010
PL2024-0010
PL2024-1010
PL2024-2010

Polymethylmethacrylate standards
Column:

Mobile Phase:
Flow Rate:
Temperature:
Detector:

2 x PL HFIPgel
PL1114-6900HFIP
7.5 x 300

3

1

HFIP + 20 mM NaTFAc
1.0 mL/min
40°C
Agilent PL-GPC 50 (RI)

1.
2.
3.
4.
5.

4
2
5

5

Retention time/min

790,000
144,000
28,900
5,720
1,020

22
VLC0040

LC AND LC/MS COLUMNS

989

GPC/SEC Columns and Calibrants

Polyethylene Glycol/Oxide
• Simple-to-use kit form
• Combines glycols and oxides to extend the MW range and cover more applications
• MWs selected to provide equidistant calibration points for greater accuracy
These hydrophilic polymers are suitable for both aqueous SEC and organic GPC using the
majority of polar organic solvents. The oxides are available in high molecular weights, while
the glycols cover the lower molecular weight range. The two types are chemically similar so
they can be used together across a wider molecular weight range, with aqueous and organic
polymers from 106-1 million MW. Every kit contains 0.2 g or 0.5 g of ten different molecular
weight standards.

Calibration Kits
Description
PEG-10, 10 x 0.5 g
PEO-10, 10 x 0.5 g

990

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Range of Nominal Mp (g/mol)
106-20,000
20,000-1,000,000

l 01357 522 961

Part No.
PL2070-0100
PL2080-0101

GPC/SEC Columns and Calibrants

Individual Polymer Molecular Weights
Polymer Nominal Mp
(g/mol)

1g
Part No.

5g
Part No.

10 g
Part No.

1.00
1.00
1.00
1.00
1.09
1.06
1.04
1.04
1.03
1.04
1.05
1.07
1.07

PL2070-1001
PL2070-2001
PL2071-2001
PL2071-3001
PL2070-3001
PL2070-4001
PL2070-5001
PL2070-6001
PL2070-7001
PL2070-8001
PL2070-9001
PL2071-0001
PL2071-1001

PL2070-1005
PL2070-2005
PL2071-2005
PL2071-3005
PL2070-3005
PL2070-4005
PL2070-5005
PL2070-6005
PL2070-7005
PL2070-8005
PL2070-9005
PL2071-0005
PL2071-1005

PL2070-1010
PL2070-2010
PL2071-2010
PL2071-3010
PL2070-3010
PL2070-4010
PL2070-5010
PL2070-6010
PL2070-7010
PL2070-8010
PL2070-9010
PL2071-0010
PL2071-1010

1.05
1.07
1.05
1.05
1.06
1.07
1.07
1.07
1.06
1.07
1.12
1.13

PL2083-1001
PL2083-2001
PL2083-3001
PL2083-4001
PL2083-5001
PL2083-6001
PL2083-7001
PL2083-8001
PL2083-9001
PL2084-0001
PL2084-1001
PL2084-2001

PL2083-1005
PL2083-2005
PL2083-3005
PL2083-4005
PL2083-5005
PL2083-6005
PL2083-7005
PL2083-8005
PL2083-9005
PL2084-0005
PL2084-1005
PL2084-2005

PL2083-1010
PL2083-2010
PL2083-3010
PL2083-4010
PL2083-5010
PL2083-6010
PL2083-7010
PL2083-8010
PL2083-9010
PL2084-0010
PL2084-1010
PL2084-2010

Nominal Mw/Mn

Polyethylene Glycol

106
194
238
282
420
600
1,000
1,500
4,000
7,000
10,000
13,000
20,000
Polyethylene Oxide

20,000
30,000
50,000
70,000
100,000
130,000
200,000
300,000
500,000
700,000
1,000,000
1,500,000

Polyethylene Glycol/Oxide standards
Column:

Mobile Phase:
Flow Rate:
Detector:

PL aquagel-OH MIXED-H 8 µm
PL1149-6800
7.5 x 300 mm, 8 µm

1.
2.
3.
4.
5.

3
4

Water
1.0 mL/min
Agilent PL-GPC 50 (RI)

2
1

1

Retention time/min

5

1,702,000
120,000
12,600
1,470
106

11
VLC0041

LC AND LC/MS COLUMNS

991

GPC/SEC Columns and Calibrants

Polysaccharides
• Comprehensive format provides full MW range in one handy kit
• Also available as individual standards
The pullulan polysaccharides kit consists of several simple sugars with relatively narrow
polydispersity linear macromolecules of maltotriose units.

Calibration Kits
Description
SAC-10, 10 x 0.2 g

Range of Nominal Mp (g/mol)
180-700,000

Part No.
PL2090-0100

Individual Polymer Molecular Weights
Polymer Nominal Mp (g/mol)
1,500
2,000
3,000
5,000
20,000
50,000
100,000
200,000
700,000
1,660,000

Unit
0.2 g
0.2 g
0.2 g
0.5 g
0.5 g
0.5 g
0.5 g
0.5 g
0.5 g
0.2 g

Part No.
PL2091-2000
PL2091-3000
PL2091-4000
PL2090-1000
PL2090-3000
PL2090-4000
PL2090-5000
PL2090-6000
PL2090-8000
PL2091-1000

Pullulan polysaccharide standards
Column:

Mobile Phase:
Flow Rate:
Detector:

4

3 x PL aquagel-OH MIXED
PL1149-6800
7.5 x 300 mm, 8 µm

1

2

3

5

1.
2.
3.
4.
5.

0.2 M NaNO3, 0.01 M NaH2PO4, pH 7
1.0 mL/min
Agilent PL-GPC 50 (RI)

8

Retention time/min

30
VLC0042

992

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961

788,000
212,000
47,300
11,800
667

GPC/SEC Columns and Calibrants

Polyethylene
• Robust particles provide reliable high temperature calibrations
• Two linear molecular weight ranges maximize choice
• Short chain branching kit, for FT-IR calibration and TREF/CRYSTAF reference
Linear polyethylene standards with low polydispersities (1.01 to 1.9) deliver accurate
GPC/SEC calibration curves, from 170-1.5 million MW. The E-MW-10 kit is recommended
for polyolefins, and is designed for direct column calibration in solvents such as trichlorobenzene
and o-dichlorobenzene from 135-180°C. Every kit contains 0.1 or 0.2 g of ten different molecular
weight standards.

Short chain branching standards
Determination of short chain branching (SCB) as a function of MWD in polyethylene is now
possible using high temperature GPC coupled with FT-IR. This series of well-characterized
polyethylene SCB standards is a valuable reference set for temperature rising elution
fractionation/crystallization analysis fractionation (TREF/CRYSTAF).

Calibration Kits
Description
E-M-10, 10 x 0.2 g
E-MW-10, 10 x 0.1 g

Range of Nominal Mp (g/mol)
170-120,000
5,000-1,500,000
Polyethylene Short Chain Branching Range of Polmyer Nominal Methyl/
1000 Total Carbons (NMR)
Calibration Kit

Part No.
PL2650-0101
PL2650-0102

E-SCB, 10 x 0.1 g

PL2650-0103

1.27-62.50

Part No.

LC AND LC/MS COLUMNS

993

GPC/SEC Columns and Calibrants

Individual Polymer Molecular Weights
Polymer Nominal Mp (g/mol)
170
282
394
540
750
1,100
2,155
14,000
32,000
120,000

Nominal Mw/Mn
1.00
1.00
1.00
1.09
1.18
1.09
1.14
1.2
1.11
1.19

Unit
1g
1g
1g
1g
1g
1g
1g
0.2 g
0.2 g
0.2 g

Part No.
PL2650-8001
PL2650-9001
PL2650-0001
PL2650-4001
PL2650-1001
PL2650-2001
PL2650-3001
PL2650-5000
PL2650-6000
PL2650-7000

1g

PL2660-7001

0.2 g
0.2 g

PL2660-8001
PL2660-9001

Polyethylene Broad MWD Individual Molecular Weights

250,000

9.50

Polyethylene Broad MWD/SCB Individual Molecular Weights

35,000
400,000

5.0
5.0

Polyacrylic Acid
• Compatible with all aqueous columns for wide applicability
• Aqueous polymers 1,000-1 million MW
• Well-characterized Mp values ensure wide utility
Calibration Kits
Description
PAA-10, 10 x 0.2 g

994

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

Range of Nominal Mp (g/mol)
1,000-1,000,000

l 01357 522 961

Part No.
PL2140-0100

GPC/SEC Columns and Calibrants

Individual Polymer Molecular Weights
0.2 g
Part No.
PL2142-3000
PL2142-5000
PL2142-6000
PL2142-7000
PL2142-8000
PL2143-0000
PL2143-2000
PL2143-3000
PL2143-4000
PL2143-5000
PL2143-6000
PL2143-7000
PL2143-8000
PL2143-9000
PL2144-0000
PL2144-1000
PL2144-2000
PL2144-3000

Polymer Nominal Mp (g/mol)
1,000
2,000
3,000
5,000
7,000
13,000
30,000
50,000
70,000
100,000
130,000
200,000
300,000
500,000
700,000
1,000,000
1,500,000
2,000,000

1g
Part No.
PL2142-3001
PL2142-5001
PL2142-6001
PL2142-7001
PL2142-8001
PL2143-0101
PL2143-2001
PL2143-3001
PL2143-4001
PL2143-5001
PL2143-6001
PL2143-7001
PL2143-8001
PL2143-9001
PL2144-0101
PL2144-1001
PL2144-2001
PL2144-3001

Methoxy Polyethylene Glycols (MPEGs)
Agilent offers a range of highly characterized, very narrow polydispersity methoxy polyethylene
glycols (MPEGs). These very pure polymers are ideal as molecular weight reference materials
or for further modification where cross-linking should be avoided.

Methoxy Polyethylene Glycols (MPEGs)
Polymer Nominal Mp (g/mol)
5,000
10,000
20000
30,000
40,000
50,000

Nominal Mw/Mn
1.03
1.05
1.05
1.06
1.06
1.06

Part No.
PL2570-5001
PL2571-0001
PL2572-0001
PL2573-0001
PL2574-0001
PL2575-0001

LC AND LC/MS COLUMNS

995

Biosolutions and Columns for Biologic Characterization

AGILENT BIOSOLUTIONS AND COLUMNS
FOR BIOLOGIC CHARACTERIZATION
Is your lab ready for the ever-increasing
number and variety of HPLC applications
for biocharacterization and analysis
of biomolecules?
Basic peptide separations. High-sensitivity, high-resolution amino acid analyses. Fast size
exclusion separations of antibodies. The number of bio-HPLC applications continues to grow at
an unprecedented rate. Agilent's durable and reproducible line of silica and polymeric bio-HPLC
columns can help meet your lab's evolving needs for performance and speed.
On the following pages, you will find key facts and specifications for the following columns
for bio-HPLC:

• Size Exclusion Columns
• Ion Exchange Columns
• Reversed-Phase Columns for Proteins
• Capillary, Nano and MicroBore Columns
• Preparative HPLC Columns
• Oligo Solutions

996

LC AND LC/MS

www.agilent.com/chem/lccolumns
www.crawfordscientific.com

l 01357 522 961



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Metadata Date                   : 2013:05:30 13:36:51-04:00
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Producer                        : Adobe PDF Library 8.0
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Page Count                      : 462

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