CG000168 Chromium Single Cell ATAC Reagent Kits User Guide Rev A

• Introduction
  • Chromium Single Cell ATAC Reagent Kits
  • Chromium Accessories
  • Recommended Thermal Cyclers
  • Additional Kits, Reagents & Equipment
  • Protocol Steps & Timing
  • Stepwise Objectives
  • Tips &Best Practices
  • Step 1
  • 1.0 Transposition
  • Nuclei ConcentrationGuidelines
  • 1.1 PrepareTransposition Mix
  • 1.2 Isothermal Incubation
  • Step 2
  • 2.0 GEM Generation & Barcoding
  • 2.1Prepare Master Mix
  • 2.2Load Chromium Chip E
  • 2.3Run the ChromiumController
  • 2.4Transfer GEMs
  • 2.5GEM Incubation
  • Step 3
  • 3.0Post GEM Incubation Cleanup & QC
  • 3.2Post GEM Incubation Cleanup – SPRIselect
  • Step 4
  • 4.0Library Construction
  • 4.1Sample Index PCR
  • 4.2Post Sample Index Double Sided Size Selection – SPRIselect
  • 4.3Post Library Construction QC
  • 4.4Post Library Construction Quantification
  • Sequencing
  • Troubleshooting
  • 6.1 GEMs
  • 6.2 Chromium Controller Errors