Installation Instructions Softwares For RNAseq Session March 2019 Windows

User Manual:

Open the PDF directly: View PDF .
Page Count: 18

Steps to download softwares for

windows for RNAseq workshop

Mohak Sharda

Nitish Malhotra

Installing MobaXterm

Type in google search bar

Click on the link.

Download the compressed file

Click on the link

on the right only.

Open the .zip file and extract to a

desired folder

Open the MobaXterm setup from the

folder where you extracted your .zip file

A shortcut of MobaXterm will be created

after finishing the setup

(all default settings)

Installing R

If you do not have R installed on your windows, please

follow the instructions ahead

Type in google search bar

Click on the

link

Open this hyperlink.

Download the hyperlink. Follow the instructions with

default settings to install R as previously done for

MobaXterm installation.

Installing Rstudio

In order for Rstudio to work, you need to have R already

installed on your system. Please go back to the previous

section and install R before proceeding.

Click on the link highlighted

Click on the non-green fill “Download” option

Click on the first link to download

Rstudio package on your system

Follow the instructions with default settings to complete the installation.

Installation of certain packages in R using Rstudio

Type the commands in blue and then press Ctrl+Enter in the Console. Wait for the commands to

complete, before the next “>” appears on console.

Note: Just pressing Enter alone will not execute the command. Press “Ctrl” and “Enter” at the

same time to execute the command.

Type commands in the console

1

2

3

4

Similarly, install statmod using console.

> install.packages(“statmod”)

The following packages will be installed in R for carrying out the

di"erential gene expression analysis:

1) edgeR

2) gplots

3) ape

4) statmod

See you all at the workshop!