MECHANICAL ADJUSTMENTS Quick Service Manual V2.2

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1SERVICE PROGRAM

SERVICE MANUAL ver 2.2

Hospitex Diagnostics 1

Quick Reference Manual

Firmware release 3.4 Software release 3.6

SERVICE MANUAL ver 2.2

1 SERVICE PROGRAM

INTRODUCTION

The following paragraphs describe how to use Service program. Service functions allow to

set all calibrations for instrument (offsets), to check photometer unit, to control pumps and

hydraulic circuit, to set barcode reader, to monitor temperature level and to create your

printing format.

When you set a new calibration, all offsets and permanent data are stored in EEPROM

memory on main CPU board.

Eeprom

memory

At start up, eprom data are uploaded in CPU RAM memory so that instrument can work

properly.

1 ADJUST LAMP

Lamp adjustment has been done at Hospitex Diagnostics factory. The correct lamp position

is achieved by producer. Only when the lamp has been defective, it’s necessary to replace it

with a new lamp, in this occasion it is very important to test lamp filament before to insert

the new lamp.

+12V

Optical fibers

Light beam

Lamp

Pull out

Figure 1 Optical group

Hospitex Diagnostics 2

SERVICE MANUAL ver 2.2

1.1 NEEDLE ARM ADJUSTMENT

Needle adjustment has been done at Hospitex Diagnostics factory. After needle

disassembly, you have to control vertical and horizontal needle positions.

Needle arm must be adjusted in such a way that the needle position is in the centre of

washing well, see Figure 2:

1. Shut down instrument;

2. To loosen screw A and B, see figure 2, in such a way you can move by hand needle

arm can freely ;

washing well

Figure 2: needle arm

3. Moving by hand, adjust needle arm length so that the needle is in the centre of the

wash position;

4. Tighten screw A and B;

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SERVICE MANUAL ver 2.2

1.1.1 Small vertical arm adjustment

Check if the needle vertical axis is set perpendicularly to the plate below, Figure 3:

1. Loosen screw C and D, see Figure 3, in such a way that the needle can be moved

freely;

5. Figure 3: needle settings

6. Turn the needle by little movement and set it in perpendicular position;

7. Tighten screw C and D;

Hospitex Diagnostics 4

90°

washing well

CD A

90°

SERVICE MANUAL ver 2.2

1.2 Service program

Figure 4 maintenance menu

To enter in Service program, from “Option” sub menu select “Mainten.” button and then

press “Test” button, see Figure 4.

Service program is divided in 11 menus listed here below:

Item Description

Calibration By this menu you can set all the mechanical calibrations for the instrument

Eprom By this menu you can display Eeprom memory configurations

Dispenser By this menu you can run preparations

Washing station By this menu you can check washing station unit

Pumps By this menu you can check washing well pumps

Temperature By this menu you can set and monitor temperature level on instrument

Reader By this menu you can check photometer unit

Cans Only for producer

Barcode By this menu you can calibrate barcode unit

Functions By this menu you can change language interface

Printer By this menu you can edit and customize your printer setup

To come back in main program press “Exit” button. Note that after Exit instrument will

reset itself.

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SERVICE MANUAL ver 2.2

1.2.1Calibration menu

Using calibration menu is possible to set or modify mechanical calibrations.

The calibration procedure means: to find the offset for tray first position under needle and

the offsets for vertical and radial needle arm positions in order to found a correct aspiring

and dispensing action.

Tray home position

Needle home

position

Tray offset,

Needle arm

radial offset,

Needle arm

vertical offset,

Tray offset

Nee dle radial

offset

Needle vertical

offset

l: steps to have tray

position 1 under needle

m: steps to turn needle

arm over tray position 1

n: steps to found

tube/bottle bottom

Figure 5: mechanical calibration

Every offset are expressed in steps and are not a physical measure. Calibration menu has

the following graphical interface, see figure Figure 6:

Figure 6 calibration menu

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SERVICE MANUAL ver 2.2

By “Previous”/”Next” buttons you can select calibration for all the parts, Figure 6 shows

calibration for reaction tray.

The upper button in “Selection Motor” sub menu select tray motor, “Needle” button select

needle arm motors.

In “Command section” pressing “Left/Right” buttons you can choose direction movement

and how many steps to move the selected motor. The same is for “Up/Down” buttons, they

are used for up and down needle arm movement.

Note that the actual motor selected is yellow highlighted.

“Send” button dispatches the command to the instrument. “Home” button recover the home

position for the selected motor.

Instrument has 3 main trays to calibrate, however consider that serum and reagent trays

have two different position to calibrate one for the external ring and the other one for inner

ring, see Figure 7, this means that instrument needs 5 logical calibration points for trays +

1 for washing well.

Serum tray 1

Serum tray 2

Reagent tray 1

Reagent tray 2

Washing well

Reaction tray

Figure 7 trays map

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SERVICE MANUAL ver 2.2

How to calibrate

The first tray to calibrate is Serum tray 1 (inner ring). You can skip to another calibration

using “Next” and “Previous” buttons. The procedure to perform are the following.

Serum and reagent trays calibration

1. Move serum tray so that the first position is under needle arm: press “Serum” button

for selecting the tray motor and move it by “Left/Right” and “Send” buttons, see

Figure 8;

2. Turn needle arm to match the centre of first serum tray position: press “Needle”

button for selecting the arm motor and move it by “Left/Right” and “Send” buttons;

Serum tray 1

Needle arm

start point

Figure 8 sample tray calibration

3. Move needle inside the first serum position up to find the bottom. To acting in this

way press “Needle” button for selecting the motor and move it by “Up/Down” and

“Send” buttons. Note that needle height inside the serum tube must be 1 mm over

bottom level.

4. Press “Save” button to save offsets configuration in eeprom memory, (see Figure 6).

5. Press “Home” to recover needle in home position.

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SERVICE MANUAL ver 2.2

After Serum tray calibration press “Next” to skip the next calibration. Reagent tray 1

(external) and 2 (inner) and Serum tray 2 calibration procedure are similar to Serum tray 1

calibration.

Note that when you move the needle inside the reagent bottle the maximum distance

between needle edge and bottle bottom should be 2-3 mm, see Figure 9:

Figure 9 needle height

Needle calibration on washing well

1. Turn the needle arm on washing well and bring the needle in the centre of washing

well: press “Needle” button for selecting the motor and move it by “Left/Right” and

“Send” buttons;

2. By “Up” and “Send” button move down the needle to find the height as in the

following drawing:

Needle

washing well

calibration

height

ring hole

Figure 10 washing well needle height

Hospitex Diagnostics 9

2 mm

reagent bottle

Needle

SERVICE MANUAL ver 2.2

3. Press “Save” button to freeze needle offset in eeprom memory.

4. Press “Home” to recover needle home position.

Reaction tray calibration

Figure 11 reaction tray calibration

1. Press “Reaction”, using “Left/Right” and “Send” buttons move reaction tray

bringing cuvette position number 10 under dry needle, see Figure 11.

2. Press “Needle”, using “Left/Right” and “Send” buttons turn needle arm to adjust

needle position over cuvette number 1 centre as in Figure 11.

3. With cuvette number 10 under dry needle, control that led D78 on instrument CPU

board is lighted ON. If not, move step by step reaction tray in order to have led D78

lighted ON, Figure 12

P82

D78

P48

CPU Board

Figure 12 CPU Board

4. Using “Up” and “Send” buttons move down the arm to find the needle height, see

Figure 13

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Reaction tray

Needle

arm

Washing station

Dry needle

SERVICE MANUAL ver 2.2

Needle

Cuvette

Reaction tray

surface

Figure 13 needle height inside cuvette

5. Press “Save” button to freeze needle offset in eeprom memory.

6. Press “Home” to recover needle home position.

When you have finished all calibrations or changed some of them press “Reset”. In this

way the new offsets stored in eeprom memory will be charged in micro controller RAM

memory.

1.2.2 Photometer calibration

Photometer unit is composed by 4 independents reading channels. This means that a set of

4 cuvette are read at the same time. After reaction tray calibration it needs to calibrate

cuvette reading position aligned with photo battery cells. Photometer calibration, means to

find the best reaction tray position to obtain the highest energy level for cuvette reading.

Figure 14 photometer calibration

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SERVICE MANUAL ver 2.2

Calibration procedure is fully automatic and it is composed by the following steps:

1. Fill with water cuvette number 1, 60, 59, 58.

2. Select “Reaction” button and align reaction tray so that the filled cuvettes are

behind photometer channels. To obtain this, bring reaction tray cuvette position

number 25 as shown in Figure 15, half covered by tray cover.

Figure 15 photometer calibration

3. Press “Start Setting” as shown in Figure 14. The automatic procedure will start

displaying a message. At the end the found offset is stored in eeprom.

4. Press “Reset”.

The photometer calibration is completed. Note that if you change reaction tray calibration

(only physical tray position) you have to recalibrate photometer reading point also.

1.3 Eprom

During calibration procedures all information are stored in a permanent memory on CPU

board: eeprom.“Eprom” menu window is shown in Figure 16. Using Eprom menu you can:

1. Write instrument serial number using “Write S/N” button

2. Store actual eeprom information (offsets) in a local PC file

(C:\EOSBF\FILE\LastEprom.PEP), using “Save Eprom on File” button

3. Download PC offsets file (LastEprom.PEP) in eeprom memory to recover the last

eeprom memory configuration, using “Load Eprom from File” button

4. To display actual eeprom offsets , using “Eprom configuration” button

5. To display a complete instrument report, using “Calibration report” button

6. Erase eeprom information by “Clear Eprom” button

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SERVICE MANUAL ver 2.2

7. Erase AD channels offset to disable reaction tray temperature control by

environment temperature sensor (Clear AD Offset)

Figure 16 Eprom menu

1.4 Dispenser

Figure 17 dispenser

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B Serum tray 2

sensitivity

controls

A Serum tray 1

sensitivity

controls

Sensitivity

threshold, n

Deeping

threshold, d

Method selection,

SERVICE MANUAL ver 2.2

Using Dispenser menu you can perform one or more preparations. During preparation the

needle aspires reagent and serum volumes from their own trays positions and dispenses

them in reaction cuvette. As shown in Figure 17, from “Sample” combo box you can select

sample position from serum tray. Selecting “Tray1” button, sample position is on the inner

ring. With “Tray2”, sample position is on external ring.

To define how many sample volume to aspire, write it in “Volume” edit box.

During the same preparation you can add up to 3 different reagents, choose reagent

positions from combo box “Reagent 1”, “Reagent 2”, “Reagent 3” and define their volumes

in “Volume” edit box (Figure 17, right side).

Selecting “Tray1” button, reagent position is on external ring, with “Tray2” is on inner

ring.

To select the cuvette to dispense in, use “Start cuvette” combo box. To choose how many

preparations to perform use “Number Preparations” combo box.

As shown in Figure 17, there are 3 buttons:

1. “Dilute”, to perform dilutions. During dilution, needle will dispense in serum tray

external ring. To select cup position, use “Start cuvette” combo box.

2. “Fill water”, to fill with water reaction cuvette. To select the first cuvette to fill use

“Start cuvette” combo box, to select how many cuvettes to fill use and “Number

Preparations” combo box.

3. “Prepare” to execute a preparation.

4. “ISE” to push samples inside ISE module position

Needle sensor thresholds

From firmware version 1.7 it is implemented a new function that allows to adjust needle

sensor sensitivity threshold for one specific reagent when the needle are detecting the

sample on serum tray 1 or serum tray 2 positions.

In this way it will be possible to set a specific sensitivity threshold for every different type

of reagent. If adjustment sensitivity is not needed, the instrument works with default

parameters.

In Dispenser menu, see Figure 17, there are A controls to set and modify threshold on

serum tray 1 and B controls to set and modify sensitivity threshold on serum tray 2.

To modify sensitivity threshold for serum tray follow this steps:

1. From combo box m select the method (reagent) that needs threshold adjustment;

2. From combo box n, select sensitivity threshold level for relevant method indicated

at point 1. The available threshold range is [70 –100], with threshold = 100 needle

sensor has the highest sensitivity;

3. From combo box d, select Deeping threshold level in the range [70%-99%].

Percentages is referred to the fMax frequency, see Figure 19. The default value is

92%. If in your test the level sensor doesn't sense the sample level, sinking deeply

into the test tube, increase the deeping threshold value.

4. Use “Load Threshold” button to activate the new threshold level indicated at point

2. Use Dispenser controls to run preparations and test threshold sensitivity level.

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SERVICE MANUAL ver 2.2

5. Use “Save Method Threshold” button to save the new threshold level selected from

combo box n for method indicated in combo box m.

6. Use “Save All Methods Threshold” to set every method with sensitivity threshold

level shown in combo box n.

7. Use “All Methods Default Threshold” button to recover all methods with default

threshold sensitivity level. Default sensitivity level is 90 for serum tray 1 and 94 for

serum tray 2.

How to adjust thresholds

When the level sensor doesn't sense correctly the sample in serum tray 1 you have to adjust

the following parameters: Deeping threshold and sensitivity threshold. The first parameter

defines the percentage of maximum frequency fMax from which the detection starts

(maximum frequency is on needle home position, see Figure 19), sensitivity threshold is

linked with the frequency variation from air to liquid.

If the level sensor fault condition is like in the case 1 of Figure 18 it means that the deeping

threshold value is under the fMin and you need to increase it, see Figure 19. When you

modify deeping threshold move by 5% step units across the range.

If the level sensor fault condition is like in the case 2 of Figure 18 you have to make the

following :

Hospitex Diagnostics 15

1 2

sample level

Figure 18

SERVICE MANUAL ver 2.2

1. Decrease the deeping threshold until the needle goes down in the sample (case1) it

means you have found the fMin frequency. Then, increase the deeping threshold

value fDTh, it must be in the range of fMax and fMin.

2. After that you have fixed the deeping threshold decrease the sensitivity threshold

(see point 2 page 14)

Rem 1: fMax, fMin parameters are connected with the reagent that you are using and have not

absolute values. You can only estimate fMax and fMin by test.

Rem 2: Let be THs the sensitivity threshold value, THs depends by reagent type Rx that

you are using: THs(Rx). The level sensor stops the needle when:

where f is the frequency and s is the space

To modify sensitivity threshold level for serum tray 2 use B controls, the way to follow are

same exposed for serum tray 1 ( A controls). Deeping threshold are not available for serum

tray 2.

Hospitex Diagnostics 16

s am ple probe

Max

Frequency

Liquid interface

space

deeping Th

ins ide the liquidout the liquid

Min

DTh

Figure 19:

 f/ sTHsRx

SERVICE MANUAL ver 2.2

2 Washing station

Figure 20 washing station menu

Use washing station menu to check all the hydraulic pumps. In “Pumps” sub menu (A)

there are 10 buttons to select the washing station needles pump, see Figure 20.

A pump check

To switch on one of the pumps:

1. press one button from A section to choose the pump;

2. press “Pump ON” to activate the pump

To stop the pump, press “Pump OFF” button (it is “Pump ON” toggled button). The

relation between buttons in A (Figure 20) section and washing tower needles is shown in

Figure 21

Hospitex Diagnostics 17

A selection buttons

for pumps

B washing

parameters

C washing test

D Washing steps

evaluation

SERVICE MANUAL ver 2.2

Aspir.1

A.Sol

Aspir.2

B.Sol

Aspir.3 Aspir.4 Aspir.5

Drywater 1 water 2

Figure 21 washing tower

B washing parameters

To test one washing cycle:

1. select the cuvette to bring under Aspr1 needle from “N. cuvette number under

Aspir. 1” combo box

2. in “A sol”, “B sol”, “water1”, “water2” combo boxes set the liquid volume for

washing (remark: the default values are fixed by producer, it is recommended don

not change them)

3. from A section button select the pumps that you want to test

4. press “Start Washing” button to start washing cycle

After this, reaction tray will bring under Aspr 1 needle the selected cuvette and the washing

station moves down with selected pumps opened.

C washing test

Using “Washing” sub menu you can test a washing cuvettes:

1. From “Start Cuvette” combo box select the first cuvette to wash;

2. From “Cuvette N.” combo box select how many cuvettes to wash;

3. From “Type” combo box select washing type, washing type is shown in Table 1.

4. To start washing press “Wash” button.

Press “Filling” button to fill with water needle hydraulic circuit or to wash needle.

Hospitex Diagnostics 18

SERVICE MANUAL ver 2.2

Washing

Type

Description

Neutral Only with H2O

A Solution A sol + H2O

B Solution B sol + H2O

Double A sol + B sol + H2O

Table 1

D washing steps evaluation

“Test” command allows to evaluate each single washing steps

The whole washing cycle can be divided in 6 steps (see Figure19) :

1. Aspir.1 + A.Sol + Aspir.2

2. Aspir.2 + Water 1 + Aspir.3

3. Aspir.3 + B.Sol + Aspir.4

4. Aspir.4 + Water 2 + Aspir.5

5. Aspir.4 + Water 2 + Aspir.5 + Dry

6. Dry

Steps 1,2,3,4,5 are evaluated by calculating the ABS of remaining coloured drops , left by

washing step

Step 6 is evaluated by calculating the effect of water drops , left by dryer , towards ABS of

a coloured solution

In order to use this procedure you have to :

Fill position 1 of serum tray 1 with concentrate potassium bichromate (K2Cr2O7)

Fill position 1 of reagent tray with a bottle full of water

Insert on position 1 of serum tray 2 an empty cup

After automatic procedure results are shown in the following form:

An error on results is shown by colouring red the cell with wrong result .

Hospitex Diagnostics 19

SERVICE MANUAL ver 2.2

Moreover form can be saved on file”.bmp” by pressing F1 on keyboard ( optional: user will

be able to input a note in the file name), all files will be saved in “C:\EosBF\File” folder.

2.1 Pumps

Figure 22 pumps menu

Using “Pumps” menu, shown in Figure 22, you can check all the washing well pumps and

refrigerator pump:

1. Press “ON” button (toggle) in “Pumps Well washing” sub menu to turn on all the

well pumps.

2. Press “ON” button (toggle) in “Pump Freezer” sub menu to turn on the refrigerator

pump connected with reagent tray.

Washing well is connected with 3 peristaltic pumps: one to wash needle, the other ones to

get out waste.

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SERVICE MANUAL ver 2.2

Figure 23 washing well

2.2 Temperature

Figure 24 temperature menu

Using Temperature menu, Figure 24, you can monitor and calibrate temperature levels on

reaction tray and needle arm preheater. “Temperature” sub menu controls reaction tray

temperature. “Needle Temperature” sub menu controls preheater needles arm, see Figure

25.

Hospitex Diagnostics 21

A Reaction tray

temperature controls

B Needle preheater

temperature controls

C Auto

Calibration

D Sinker Test

E Temperature

Factor

SERVICE MANUAL ver 2.2

Preheater

Figure 25 preheater

A Reaction tray temperature controls

To display actual reaction tray temperature press “T.MAX” button, software will show

temperature digital level in “Digital level” edit box. To calibrate reaction tray temperature

you have to define a digital level Target. To change “Target” level use “Up” button (to

increase) “Down” (to reduce) and “Send” to modify the displayed target value. Actual

“Digital Level” and “Target” values are expressed in digital units and are not physical

degrees (°C).

Note : When environment temperature sensor is set up on equipment (see 1.5 )

When user press “Save” button , equipment will also store environment temperature and

Reaction tray temperature target will be automatically modified according to that

environment .During temperature calibration , by pressing F1 on keyboard , environment

temperature , sinker temperature(if present) and adjusted target can be shown .

The automatic correction of target temperature is controlled by a temperature factor

(default: 65 m°C/°C) that makes target decrease when environment temperature increase.

The automatic correction uses a linear model , so, in order to perform good corrections,

it’s strongly recommended to calibrate reaction tray temperature with an

environment temperature from 22°C to 26.5°C .

That’s because the linear model is working good only with small variation (positive or

negative) around a medium point , so it’s recommended to fix this medium point not with

too low temperature and not with too high temperature

Hospitex Diagnostics 22

SERVICE MANUAL ver 2.2

Moreover in order to assure a right working of temperature control sinker temperature

must be around 30°C/40°C.

In order to disable reaction tray temperature control by environment temperature ,

command “Clear AD Offset” on “Eprom” menu has to be used.

B Needle preheater temperature controls

To find the target value corresponding at 37,5 °C, fill with water a cuvette and insert inside

a thermometer probe. You have to wait that reaction tray temperature reaches the target

value, after this, control the Celsius degrees on tray with thermometer. If temperature is not

37,5°C you have to modify target value using “Up/Down” buttons.

When you have found the right target value save it in eeprom memory pressing “Save”

button.

37°

Reaction tray

Figure 26 reaction tray temperature test

Press “Start Read” to monitor actual digital level on preheater and target value. About

calibration you have to follow this procedure:

1. turn on reagent tray refrigerator and wait for a low temperature.

2. Wait for 37,5°C on reaction tray.

3. Using “Dispenser” menu, you have to run a preparation in which needle aspires

400 ul of water from reagent tray and 4 ul from serum tray and dispense it in a

reaction tray cuvette. Repeat this operation 3 times.

4. Insert inside the last dispensed cuvette a thermometer probe.

5. For a good preheater target, water should reach 37 °C in 3 minutes

6. Using “Up” and “Down” buttons, modify target value if is not as point 5

7. Press “Save” button to save new target value.

Hospitex Diagnostics 23

SERVICE MANUAL ver 2.2

Figure 27 temperature rate

To exit from “Temperature” menu, press “Stop” button.

C Temperature auto calibration

In order to perform this procedure it’s necessary to use tester “Lafayette MAS-345” with

communication serial port RS232 connected with an NTC sensor.

Fill a cuvette with 500uL of water

Put this cuvette on reaction tray position under photometer mask

Switch tester on Ohm mode

Connect NTC with tester probes

Connect Tester on PC Com1

Push “Auto” on “Temperature” menu

Wait for end of procedure ( it can last from 40 min to 60 min)

During procedure, calibration can be stopped by pressing ESC on keyboard , note that in

this case current level and environment temperature will be saved.

Moreover environment temperature and sinker temperature can be shown by pressing F1 on

keyboard and current cuvette temperature is shown on a message.

Hospitex Diagnostics 24

Temperature [°C]

Time

3 min

37°

30°

SERVICE MANUAL ver 2.2

If temperature calibration end with no error software will show :

TEMPERATURE ON CUVETTE + SAVED DIGITAL TARGET

If temperature calibration end with error software will show an error code :

TEMPERATURE CAN NOT BE CONTROLLED : after first correction

temperature is not reaching for target

TEMPERATURE CAN NOT REACH PLATEAU : after a correction temperature

doesn’t become stable but keeps on drift

TEMPERATURE CAN NOT REACH TARGET : after maximum number of

corrections target is not reached

TEMPERATURE CAN NOT STAND BY ON TARGET: after target is reached

temperature keeps on changing around target

D Sinker test

Sinker efficacy can be controlled with an automatic procedure.

Some temperature cycles are performed on sinker and the rising temperature time is

monitored.

This procedure may last several minutes( from 40 to 60) and it’s recommended to perform

it when equipment has been just switched on.

Procedure can be stopped by pressing ESC on keyboard.

If sinker temperature test end with no error software will show :

RISING TEMPERATURE SPEED+ LAST TEMPERATURE

If sinker temperature end with error software will show an error code :

FREEZE ERROR+ LAST TEMPERATURE : sinker can not get cold

WARM ERROR+ LAST TEMPERATURE : sinker can not get hot

RISING TEMPERATURE SPEED+ LAST TEMPERATURE : the rising speed is

too low

E Temperature factor

Temperature factor (m°C/°C) is the variation of reaction tray temperature (m°C) according

to a 1°C variation of environment temperature.

The default factory value is 65 m°C/°C

Hospitex Diagnostics 25

SERVICE MANUAL ver 2.2

It’s possible to change temperature factor on software by command on menu temperature

If command is pressed when text box is cleared , current factor will be shown

If command is pressed when text box is filled , written factor will be saved on

board

It’s strongly recommended to not change this factor .

This factor may be changed only in some particular cases :

Special environment

Special working conditions

Special equipment configurations

………………..

Hospitex Diagnostics 26

SERVICE MANUAL ver 2.2

2.3 Reader

Using “Reader” menu you can check photometer channels and all the filters.

Figure 28 reader menu

In the “Set” sub menu you have the following input boxes and buttons:

1. “Channel N.” to select the channel [1 ÷ 4] .

2. “Cuvette N.” to select the cuvette [1 ÷ 60]. Reaction try will bring the cuvette

aligned with selected channel (point 1).

3. “Filter” to select the actual reading filter (340 nm, 405 nm, 492 nm, 505 nm, 546

nm, 578 nm, 630 nm, 700 nm).

4. Select “Tension” button to display reading results in voltage levels (mV) or “Abs”

button to display absorbance reading results

5. Press “Reading” (toggle mode) button to run reading operation and start monitoring.

6. Press “Stop” to end reading operation.

If you select “Channel N” = 1 and “Cuvette N.” = 1, after press “Reading” button

(“Reading” toggle “Stop”), reaction tray position will be as in Figure 29. For a

coherent reading operation cuvettes must be filled with water. Photometer unit reads 4

cuvettes at the same time, this means that you have to fill cuvette numbers 1, 60, 59, 58, see

Figure 29.

During reading operation channels results will display in “Read” sub menu edit boxes.

Hospitex Diagnostics 27

SERVICE MANUAL ver 2.2

Figure 29 reading position alignment

If you select “Abs” button, software will display absorbance reading results. Selecting

“Abs” mode, if you press “Zero” button and then “Graphic”, software will open a graphical

display to control photometer channels reading rate, Figure 30.

Figure 30 reading graphical display

Hospitex Diagnostics 28

158

Photo battery

Ch1 Ch2 Ch3 Ch4

Reaction tray

washe r

Photometer

Optical fibers

SERVICE MANUAL ver 2.2

2.3.1 Replacement function

In order to test replacement error effect on photometer performance , an automatic

procedure can be performed.

This procedure needs diluted 1/100 potassium bichromate K2Cr2O7 (ABS around 1.700)

Fill bottle on reagent tray position 1 with diluted 1/100 potassium bichromate

Start procedure with “Replacement” command

Wait for end procedure

At the end of procedure results are shown in a form :

By pressing buttons 1, 2, 3... and so on a graphic of reading can be shown:

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SERVICE MANUAL ver 2.2

Each of 12 cuvette are filled with solution and they are read 5 times after moving reaction

tray.

The procedure can estimate:

Replacement reading error between each reading of a cuvette: if some cuvette

reading are too different the label under Error column becomes red

Accuracy error between each channel of photometer : if then mean reading of

each channel is too different in comparison with the other the results labels

becomes red

Drift rate between each reading of a cuvette : if cuvette reading drift is too high

the label under Drift column becomes yellow

Moreover form can be saved on file”.bmp” by pressing F1 on keyboard ( optional: user will

be able to input a note in the file name), all files will be saved in “C:\EosBF\File” folder.

2.3.2 Photometer adjustment

In order to realize a photometer adjustment it is necessary that the photometric calibration

has been executed first as described before.

Photometer check is as follows:

1. Ensure that photometric calibration is OK, then read the voltage level;

8. At 340 nm wavelength the average voltage level must be in the range of [400 ÷

1000] mV;

9. Maximum variance for each channel from average energy level must be ± 20%;

10. For all the other filters, maximum energy level must be under 4100 mV, consider

that 5000 mV energy level is the photometer saturation;

To change channel energy levels :

Open instrument on lateral side, below photometer unit there are 4 screw

for gain trimming, Figure 32 .

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SERVICE MANUAL ver 2.2

Select the channel out of range and turn the screw to adjust the gain so

that channel voltage level will be in the optimal range (for this operation

you have to run photometer reading with filled cuvette and control

channels monitor), Figure 31.

ADC

CH1

CH2

CH3

CH4

Trimmer gain

Figure 31 trimming

Ch1 screw hole

Ch4 screw hole

connectors

Photometer unit lower view

Reaction tray

Photo battery

Hospitex Diagnostics 31

SERVICE MANUAL ver 2.2

Figure 32: photometer below layer

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SERVICE MANUAL ver 2.2

2.4 Barcode

Figure 33 barcode menu

Figure 33 shows Barcode calibration menu. In right side, you have some general buttons:

1. “On” button to start barcode reading.

2. “Off” button to stop barcode reading.

3. “Reagent Tray” button to move one position in reagent tray.

4. “Serum Tray” button to move one position in serum tray.

5. “Home Tray” button to recover reagent and serum tray home positions.

Reference label is on reagent tray, before barcode reader. With empty reagent tray, if you

press “On” button barcode reads reference label and a message with the read value should

appears (reference label value is “0000”). If the message doesn’t appear, it means that

barcode has some reading problems and you have to adjust its physical position, see Figure

34:

Hospitex Diagnostics 33

Serum controls

sub menu

Reagent controls

sub menu

General control

buttons

Display window

Time parameters

Trays speeds

SERVICE MANUAL ver 2.2

Reagent reading window

R35

Serum reading window

Barcode reader

Reference label

"0000"

radial offset

Home positions

Home Barcode steps

offsets for reagent and

serum trays

Figure 34 barcode reader

To calibrate barcode, open lateral and front cover.

Barcode calibration procedure:

1. Insert offsets values (steps) in “Home Barcode Steps” edit boxes (Figure 33) to have

windows reading position aligned with barcode reader, see Figure 34. Then, press

“Home Barcode” button to test the new offsets: reagent and serum trays will move

them self to bring reading windows in front of barcode. Repeat this operation to find

the best offsets, thus press “Save” button.

2. “Serum Offset” and “Reagent Offset” are steps distances to have sample and

reagent first positions in front of barcode reader, see Figure 35. To test offsets, first

insert tube and bottle in their relevant position with barcode label, then press

“Offsets” button: reagent tray will bring the bottle in reading position. If you have

chosen a good offset a message will appear to display the read code, then press

“OK” message button. The same operation is for serum tray. Repeat this operation

to find the best offsets, thus press “Save” button.

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SERVICE MANUAL ver 2.2

Reading windows

R35

Barcode reader

Offsets

Figure 35 offsets calibration

3. Tray speed edit box: you can select tray speed in the range [190 – 90] rpm, the

default values is 150 rpm, see Figure 33.

4. Time parameters: “Back-On”, “On-Off” and “Off-On” values, to calculate them

press “Reagent Calibrate” button for reagent tray and “Serum Calibrate” for serum

tray.

A fully automatic procedure will start to compute time parameters. It’s necessary to

have serum tube and reagent bottle with calibration code. Calibration code is

defined in Main program: press “Functions” button, then open “Barcode” menu,

here you can edit or modify calibration code. Physical meaning of time parameters:

start

Off-On

Back-On

Second position

reading time

First position

reading time

On-Off

+ T

Off-On

= K

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SERVICE MANUAL ver 2.2

TBack-On: time to wait first label in reading position from starting point

TOn-Off: time to read one label

TOff-On: delay for the next reading

If you change some computed values, remember that TOn-Off and TOff-On sum

must be a constant

5. Insert some labelled bottles in reagent tray, then press “ReagentCheck” button for a

complete barcode scan. In Display window “Reag” will appear scan results, thus

control them with physical dispositions. If the test is not OK, repeated reagent

calibration as previous point 4 or modify time parameters. Otherwise press “Save”

button.

6. Insert some labelled tubes in serum tray, then press “SerumCheck” button for a

complete barcode scan. In Display window “Ser” will appear scan results, thus

control them with physical dispositions. If the test is not OK, repeated serum

calibration at previous point 4 or modify time parameters. Otherwise press “Save”

button.

2.5 Functions

Figure 36 functions menu

Use “Functions” menu

To select interface language in “Language” sub menu.

To pilot ISE module in “ISE” sub menu.

To move dilutor in “Dilute” sub menu.

To set start up temperature in “Reaction Temperature” sub menu

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SERVICE MANUAL ver 2.2

To perform stress procedure in “Special Op.” sub menu

To set cover type of equipment in “Cover.” sub menu

2.5.1 Testing Tubes with Dilutor

Using Dilute menu on “Functions” menu It’s possible to test heater Teflon tubes and needle

volumes.

Needle Test

Perform filling on “Washing Station” menu until hydraulic is

completely filled

On submenu “Dilute” select “Down” and input 450 on text box ,

select “Needle” and press “Send”

Verify that water column is nearly outside needle.

If not, needle has not right volume

Press “Home”

Preheater

Heater Teflon hydraulic Test

Hospitex Diagnostics 37

Testing

Tubes

SERVICE MANUAL ver 2.2

Perform filling on “Washing Station” menu until hydraulic is

completely filled

On submenu “Dilute” select “Down” and input 1900 on text box ,

select “Needle” and press “Send”

Select “Water”, select “Up” and press ”Send”

Select “Needle”, select “Down” and press ”Send”

Selezionare NEEDLE, selezionare DOWN e premere SEND.

Verify that water column is nearly outside heater.

If not, heater Teflon hydraulic has not right volume

Press “Home”

Preheater

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SERVICE MANUAL ver 2.2

2.6 Printer

You can edit your printing format using Printer menu, see Figure 37. Before to enable

printer option, install your printer on PC instrument. In default option, printer is not

enabled.

After printer general enable, you can add logo to your format by “Image” selection and a

printing header by “Header” function.

Common printing functions are available in Options area.

In patient data you can select different features.

Figure 37 printer menu

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General enable

Options

SERVICE MANUAL ver 2.2

3 PUMP SUBSTITUTION

To replace one of the peristaltic pumps on the side of instrument pull out the pump head

and unscrew the two tubes, see the below figure:

x x

HEAD

TUBE

Figure 38: Peristaltic pump

Note: do not intertwine the lateral tubes when you reinsert the pump.

4 AD lines

Equipment main board has 4 AD lines :

1. Reaction Tray temperature……………………………………P42

2. Needle Heater temperature……………………………….P43

3. Environment temperature……………………………P44

4. Sinker temperature…………………………P45

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SERVICE MANUAL ver 2.2

Lines 1 and 2 are used to control temperature , that’s because they have a gain(around

10) that makes reading very accurate.

Lines 3 and 4 are used just to monitor temperature(environment and sinker) , so they

have no gain. In order to have good reading on these lines it’s necessary to calculate

offset. Software uses an automatic procedure to evaluate offset on line 3 and 4

Be sure that sensor is connected on P44

Be sure that sensor is connected on P45

 Switch on equipment

Use Service program on menu “Temperature”

Push “TMAX”button

Press F1 on keyboard (environment and sinker temperature will appear)

Disconnect sensor on P44 and wait for environment temperature monitor

becomes zero

Connect sensor on P44 again and wait temperature monitor reaches real value

starting from zero

 Disconnect sensor on P45 and wait for sinker temperature monitor becomes

zero

Connect sensor on P45 again and wait temperature monitor reaches real value

starting from zero

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SERVICE MANUAL ver 2.2

Notes :

Reaction tray temperature control according to environment temperature is

disabled if this procedure is not properly performed .

In order to clear current offsets use “Clear AD Offset” button on “Eprom”

4.1 Hidden Keys

ESC : Reaction tray temperature auto calibration and sinker test can be suddenly

stop , by pressing ESC key ( see 1.3.7 section ) . Note that during auto calibration

current level and environment temperature will be saved.

F1 : During reaction tray temperature auto calibration and manual calibration ,

environment and sinker test can be shown by pressing F1 key ( see 1.3.7 section).

F11 : Service software allows user to monitor equipment working time by

pressing F11 key on each menu

F12 : Service software allows user to “reset & start again program” time by

pressing F12 key on each menu . This function is very important to unlock software

any time it’s necessary.

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MECHANICAL ADJUSTMENTS Quick Service Manual V2.2

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