Seahorse XF Cell Mito Stress Kit User Guide

• Contents
• Introduction
  • Assay Background
  • Glossary
• Kit Information
  • Kit Contents
  • Kit Shipping and Storage
  • Additional Required Items
• Assay Workflow
  • Day Prior to Assay
  • Day of Assay
    • Prepare assay medium
    • Removing reagent caps
    • Prepare stock compounds
    • Prepare compounds for loading in sensor cartridge
    • Load sensor cartridge
    • Prepare Agilent Seahorse XF Cell Culture Microplate for assay
  • Running the Assay
  • Data Analysis