Microwave Instructions For Agarose Preparation Gsbbwan

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Microwave Instructions for Agarose Preparation (for
Denville® Agarose LE)
Denville Scientific

Abstract
Denville LE Agarose is an all purpose agarose for routine nucleic acid electrophoresis of
fragments between 500bp-23,000 bp.

Denville LE Agarose has no detectable DNase or RNase activity.
Citation: Denville Scientific Microwave Instructions for Agarose Preparation (for Denville® Agarose LE). protocols.io
dx.doi.org/10.17504/protocols.io.gsbbwan
Published: 16 Dec 2016

Guidelines
Introduction
Denville LE Agarose is an all purpose agarose for routine nucleic acid electrophoresis of fragments
between 500bp-23,000 bp. Denville LE Agarose has no detectable DNase or RNase activity.

Analytical Specifications
Gelling temperature (1.5%)

36°C ±1.5°C

Melting temperature (1.5%)
Gel strength (1%)

>90°C
>1,200 g/cm²

Applications

Analytical electrophoresis of DNA and RNA >1,000 bp
Blotting of DNA and RNA

Suggested Agarose Concentrations
1

Published: 16 Dec 2016

This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are credited

Dye Mobility Table
Migration of double-stranded DNA in relation to Bromophenol Blue (BPB) and Xylene Cyanol (XC) in
Denville LE Agarose Gels.

Materials
Agarose GR140-500 by Denville Scientific Inc.

Protocol
Microwave Instructions for Agarose Preparation
Step 1.
2

Published: 16 Dec 2016

This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are credited

Choose a beaker that is 2-4 times the volume of the solution
Microwave Instructions for Agarose Preparation
Step 2.
Add room temperature 1X or 0.5X electrophoresis buffer and a stir bar to the beaker.
Microwave Instructions for Agarose Preparation
Step 3.
Slowly sprinkle in the agarose powder while the solution is rapidly stirred.
Microwave Instructions for Agarose Preparation
Step 4.
Remove the stir bar if not Teflon® coated.
Microwave Instructions for Agarose Preparation
Step 5.
Weigh the beaker and solution before heating.
Microwave Instructions for Agarose Preparation
Step 6.
Cover the beaker with plastic wrap.
Microwave Instructions for Agarose Preparation
Step 7.
Pierce a small hole in the plastic wrap for ventilation.
Microwave Instructions for Agarose Preparation
Step 8.
Heat the beaker in the microwave oven on High power until bubbles appear.
Microwave Instructions for Agarose Preparation
Step 9.
Remove the beaker from the microwave oven. Caution: Any microwaved solution may become
superheated and foam over when agitated.
Microwave Instructions for Agarose Preparation
Step 10.
GENTLY swirl the beaker to resuspend any settled powder and gel pieces.
Microwave Instructions for Agarose Preparation
3

Published: 16 Dec 2016

This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are credited

Step 11.
Reheat the beaker on HIGH power until the solution comes to a boil.
Microwave Instructions for Agarose Preparation
Step 12.
Hold at boiling point for 1 minute or until all of the particles are dissolved.
DURATION
00:01:00
Microwave Instructions for Agarose Preparation
Step 13.
Remove the beaker from the microwave oven.
Microwave Instructions for Agarose Preparation
Step 14.
GENTLY swirl the beaker to thoroughly mix the agarose solution.
Microwave Instructions for Agarose Preparation
Step 15.
After dissolution, add sufficient hot distilled water to obtain the initial weight.
Microwave Instructions for Agarose Preparation
Step 16.
Mix thoroughly.
Microwave Instructions for Agarose Preparation
Step 17.
Cool the solution to 50°C-60°C prior to casting.

Warnings
Always wear eye protection when dissolving agarose and guard yourself and others against scalding
solutions.

Refer to Material Safety Data Sheet for additional safety and handling information.

4

Published: 16 Dec 2016

This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are credited
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