Sc 32251
User Manual: 32251
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SANTA CRUZ BIOTECHNOLOGY, INC.
α-Actin (1A4): sc-32251
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
BACKGROUND
All eukaryotic cells express Actin, which often constitutes as much as 50%
of total cellular protein. Actin filaments can form both stable and labile struc-
tures and are crucial components of microvilli and the contractile apparatus
of muscle cells. While lower eukaryotes, such as yeast, have only one Actin
gene, higher eukaryotes have several isoforms encoded by a family of genes.
At least six types of Actin are present in mammalian tissues and fall into three
classes. α-Actin expression is limited to various types of muscle, whereas β-
and γ- are the principle constituents of filaments in other tissues. Members
of the small GTPase family regulate the organization of the Actin cytoskele-
ton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac
regulates Actin filament accumulation at the plasma membrane and Cdc42
stimulates formation of filopodia.
CHROMOSOMAL LOCATION
Genetic locus: ACTA2 (human) mapping to 10q23.31; Acta2 (mouse) mapping
to 19 C1.
SOURCE
α-Actin (1A4) is a mouse monoclonal antibody raised against amino acids
1-10 mapping at the N-terminus of smooth muscle α-Actin of human origin.
PRODUCT
Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1%
sodium azide and 0.1% gelatin.
α-Actin (1A4) is available conjugated to agarose (sc-32251 AC), 500 µg/
0.25 ml agarose in 1 ml, for IP; to HRP (sc-32251 HRP), 200 µg/ml, for
WB, IHC(P) and ELISA; to either phycoerythrin (sc-32251 PE), fluorescein
(sc-32251 FITC), Alexa Fluor®488 (sc-32251 AF488), Alexa Fluor®546
(sc-32251 AF546), Alexa Fluor®594 (sc-32251 AF594) or Alexa Fluor®647
(sc-32251 AF647), 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either
Alexa Fluor®680 (sc-32251 AF680) or Alexa Fluor®790 (sc-32251 AF790),
200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM.
Alexa Fluor®is a trademark of Molecular Probes, Inc., Oregon, USA
APPLICATIONS
α-Actin (1A4) is recommended for detection of smooth muscle α-Actin of
mouse, rat and human origin by Western Blotting (starting dilution 1:200,
dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of
total protein (1 ml of cell lysate)], immunofluorescence (starting dilution
1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin-
embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and
solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000); non
cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium
(α-myocardial), and striated muscle (α-sarcomeric).
Suitable for use as control antibody for ACTA2 siRNA (h): sc-43590, ACTA2
siRNA (m): sc-43591, ACTA2 shRNA Plasmid (h): sc-43590-SH, ACTA2
shRNA Plasmid (m): sc-43591-SH, ACTA2 shRNA (h) Lentiviral Particles:
sc-43590-V and ACTA2 shRNA (m) Lentiviral Particles: sc-43591-V.
Molecular Weight of α-Actin: 43 kDa.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
DATA
SELECT PRODUCT CITATIONS
1. Jacque, J.M., et al. 2006. The inner-nuclear-envelope protein emerin
regulates HIV-1 infectivity. Nature 441: 641-645.
2. Lanuti, P., et al. 2006. Parallel regulation of PKC-αand PKC-δcharacter-
izes the occurrence of erythroid differentiation from human primary
hematopoietic progenitors. Exp. Hematol. 34: 1624-1634.
3. Yuecheng, Y., et al. 2006. Clinical evaluation of E-cadherin expression and
its regulation mechanism in epithelial ovarian cancer. Clin. Exp. Metastasis
23: 65-74.
4. Cheng, G.S., et al. 2017. Bone marrow-derived mesenchymal stem cells
modified with IGFBP-3 inhibit the proliferation of pulmonary artery
smooth muscle cells. Int. J. Mol. Med. 39: 223-230.
5. Di Gregorio, J., et al. 2017. Role of glycogen synthase kinase-3βand
PPAR-γon epithelial-to-mesenchymal transition in DSS-induced colorectal
fibrosis. PLoS ONE 12: e0171093.
6. Da Ros, M., et al. 2017. FYCO1 and autophagy control the integrity
of the haploid male germ cell-specific RNP granules. Autophagy 13:
302-321.
7. Pan, X., et al. 2017. Mice, double deficient in lysosomal serine carboxypep-
tidases Scpep1 and Cathepsin A develop the hyperproliferative vesicular
corneal dystrophy and hypertrophic skin thickenings. PLoS ONE
12: e0172854.
8. Li, R., et al. 2017. Self-assembled N-cadherin mimetic peptide hydrogels
promote the chondrogenesis of mesenchymal stem cells through inhibi-
tion of canonical Wnt/β-catenin signaling. Biomaterials 145: 33-43.
9. Feng, D., et al. 2017. Expression and alteration of BKCa channels in the
sphincter of Oddi’s from rabbits with hypercholesterolemia. Channels.
E-published.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
α-Actin (1A4) HRP: sc-32251 HRP. Direct western blot
analysis of α-Actin expression in C2C12 (A), A-10 (B),
NIH/3T3 (C), BC3H1 (D) and Sol8 (E) whole cell lysates
and human heart tissue extract (F).
107 K –
77K–
48K–
ABC DE F
<α-Actin
α-Actin (1A4): sc-32251. Immunoperoxidase staining
of formalin fixed, paraffin-embedded human smooth
muscle tissue showing cytoplasmic and cytoskeletal
staining of smooth muscle cells.
