Fujifilm Las 3000 Users Manual

LAS-3000 to the manual 8eebdba3-11f9-40d8-bd4a-46becd8d4f9d

2015-02-02

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FUJINON LENS
SUPER CCD
COMPACT BODY

Compact, but packed with Proprietary
Equipped with a high-quality,
cooled CCD camera and a compact
image-analysis system

◎ A digital system dispensing with dark rooms, films, and chemicals
◎ High-quality images resulting from high sensitivity and smoothing function
◎ Reduced exposure time due to multiple binning modes
◎ Easy operation improved by Image Reader software

Super CCD chip affords up to 6.3 million pixels
By rotating pixels 45 degrees to form an interwoven layout, the Super CCD's pixel
pitch in the horizontal and vertical directions is narrower than in the diagonal direction,
achieving higher horizontal and vertical resolution.

Super CCD chip

Octagonal pixel-interwoven layout

Equipped with a newly designed large-aperture F0.85 lens
The analyzer incorporates a FUJINON, a


 


strikingly bright lens with an F-number of
0.85. This lens has been especially designed to make full use of the advantages
of Fujifilm's proprietary Super CCD chip,
and is excellent for capturing images from
distances as short as several tens of centimeters. In its design, optical expertise
developed through professional applications such as broadcasting TV cameras is
fully exploited.

	 	

!

FUJINON LENS VRF43LMD F0.85

High-grade performance in a compact body
The same grade of camera and lens as in LAS-3000, the highest-grade model of
the LAS series, are used.

By limiting the applications to the specific areas of

chemiluminescence and bioluminescence, a compact body is made possible while
still retaining the high resolution and high performance of LAS-3000. Free from the
necessity for dark rooms and chemicals, the analyzer can be placed on the top or
side of an ordinary desk, and requires no special expertise for operation.

 	
 

 	 



 


LAS-3000mini Image Reader running on a PC can be used to specify image capture
parameters. You can specify image capture parameters, such as the exposure method,
exposure time, sensitivity, resolution, etc., according to instructions given on the screen.
You can specify the settings of the detection method and diaphragm in accordance with
the recommendations on the screen of Method / Tray position. Manual setting is not
needed for any of the image corrections required for quantitative analysis, which is
carried out automatically.

Screen of Method/Tray position

Screen of Exposure Increment

Expertise in Bioscience
Dedicated to luminescence applications,
and particularly suitable for Western blotting applications
A compact image analysis system dedicated to chemiluminescence/bioluminescence applications,
the perfect standard system for Western blotting applications.

■ Applicable reagents
● Chemiluminescence:CDP-Star ® , ECL PlusTM , ECLTM , SuperSignal® , ImmunoStar, CSPD® , etc.
● Bioluminescence :Luciferase, etc.

■ Sample images

 * '  *      !

■ Calcium-binding protein expressed in the rat small
  intestine, detected by Western blotting

■ AtlasTM Plastic Mouse 5K Microarray SpotLightTM
  Chemiluminescent Hybridization & Detection kit

◎  	  "# $
◎
 
 	 %&

◎
 
 	 %&

■ Mouse IgG detected by Western blotting

■ Human IgG on a titer plate, detected by ELISA

※#  '  '   (
 )*   +

◎  	 

 
◎
 
 	 

 	 


	       !
◎

Optional Products
■ LAS-3000mini UV-Bay set

■ Non-parallax (NP) tray

Combination usage of a UV-Bay set and any
additional UV transilluminator enables the
LAS-3000mini to capture UV transilluminated
images.

A non-parallax (NP) tray is used to eliminate the parallax
effect when imaging chemiluminescence in the 96-well
plate. Up to two plates can be placed.

Includes;
・housing box
・605DF40 optical filter for EtBr detection
*Requires additional UV transilluminator.

LAS-3000mini Basic Specifications

●Chemiluminescence

 Number of pixels     : 3.2 million pixels

●Bioluminescence

 Pixel size         : Approx. 11 μm

Operating conditions

 Cooling temperature    : Down to -30゜
C(When room temperature is below 28℃.)
 Dynamic range      : Four orders of magnitude
 Focusing and diaphragm : Manual
 Gradation        : 16 bits
 Exposure mode      : Automatic / manual (normal / incremental / repetitive)
 Exposure time      : Automatic / manual (1 / 100 seconds to 30 hours)

TM

TM

SuperSignal ® , ImmunoStar, CSPD® , etc.

 CCD chip        : Fujifilm Super CCD Area Type chip

 Cooling         : Two-stage thermoelectric module with air circulation

Analyzing Unit(separate product)
: CDP-Star ® , ECL Plus , ECL ,
: Luciferase

●Line frequency 50 / 60 Hz
●Guaranteed operating conditions
 Temperature

: 15 - 28゜
C,

 Humidity

: 30 - 70% (no condensation)

●Power Consumption

: Approx. 0.2 kVA

Main body dimensions and mass

 Pixel correction      : Dark-frame correction, flat-frame correction,

●Dimensions

              distortion correction, etc.

 Camera head and dark box : 380 (W) × 660 (H) × 370 (D) mm
 Controller

 Image quality correction : Binning and smoothing

●Weight

 Read pixel size      : Down to 35 μm

 Camera head and dark box : Approx. 20.2 kg

 Maximum sample size   : 18 × 12 cm

 Controller

Optional Products

●Intelligent Dark Box VI

: Windows® XP / Mac OS X

 OS

TM

●Analysis software ScienceLab

< Image capturing unit >
380
Camera head and
dark box

80

Controller

: 80 (W) × 386 (H) × 335 (D) mm

 Image size       : Up to 12 MB (formats : FUJI and TIFF)

 Interface         : USB 1.1

●Computer

386

Applicable reagents

●Camera head and Controller

170

Image Capturing Unit(Requires additional Analyzing Unit)

490

■ Specifications and Configuration

: Approx. 5 kg

Desk is not included.
unit (mm)

< Analyzing unit >

●Non-parallax tray

●Dedicated tray

: For chemiluminescence

●Lens

: High-sensitivity lens :
FUJINON VRF43LMD

●UV-DIA tray
F0.85

Remarks
●Gel documentation is available

Windows ¨ XP or MacTM OS X

■ No license is granted for fluorescent dye pre-labeled gel electrophoresis with UV transilluminator.
■ No license is granted for use of AttoPhos TM to detect nucleic acid on a nylon membrane.

Fuji Photo Film Co., Ltd. 26-30, Nishiazabu 2-Chome, Minato-ku, Tokyo 106-8620, Japan, Tel: + 81-3-3406-2201, Fax : +81-3-3406-2158・E-mail : sginfo@fujifilm.co.jp
Fujifilm Medical Systems U.S.A., Inc. 419 West Avenue, Stamford, CT 06902, U.S.A. Tel: +1-203-324-2000 ext. 6112 (1-800-431-1850 ext. 6112 in the U.S.) Fax : +1-203-351-4713・E-mail : SSG@fujimed.com
Fuji Photo Film (Europe) GmbH, Heesenstr. 31, 40549 Dusseldorf, Germany, Tel: +49-211-5089-174, Fax : +49-211-5089-139・E-mail : lifescience@fujifilmeurope.de
Fuji Photo Film (U.K.) Ltd. Unit 12 St Martins Way, St Martins Business Centre, Bedford, MK42 OLF, U.K , Tel: +44-1234-245291, Fax : +44-1234-245293・E-mail : lifesciences@fuji.co.uk
Fuji Photo Film (China) Investment Co., Ltd. 31st floor, Hong Kong New World Tower, No.300 Huai Hai Zhong Road, Shanghai, P.R China, Tel: +86-21-3302-4655 ext.363, Fax : +86-21-6384-3322・E-mail : wgxiang@fujifilm.com.cn

http: // lifescience.fujifilm.com
Specifications and system configuration subject to change for improvement without notice. All other product names mentioned herein are the trademarks of their respective owners.

宣−05・12−
(F1099)
・5−1(F1099) 宣伝物コード6007 1580



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