Leica Microscope And Magnifier M125 B Users Manual

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Living up to Life

Leica M125 B

Leica M165 C B

User Manual

Leica M series User Manual 2

All rights to this documentation are held by

Leica Microsystems CMS GmbH. Reproduction

of text or illustrations (in whole or in part) by

print, photocopy, microfilm or other method

(including electronic systems) is not allowed

without express written permission from Leica

Microsystems CMS GmbH.

The instructions contained in the following

documentation reflect state-of-the-art tech-

nology. We have compiled the texts and illus-

trations as accurately as possible. Still, we are

always grateful for comments and suggestions

regarding potential mistakes within this docu-

mentation.

The information included in this manual may

be changed without prior notice.

Copyright

Revision 1.0, published March 14, 2013 by:

Leica Microsystems Wetzlar GmbH

Ernst Leitz-Straße 17-37

D-35578 Wetzlar (Germany)

http://www.leica-microsystems.com

Responsible for contents:

Marketing CMS

Leica M series User Manual 3

Function of the Microscopes

The Leica M125 B and Leica M165 C B micro-

scopes, for which this User Manual has been

written, are designed for routine examinations

of cell and tissue cultures, liquids, and sedi-

ments. This includes examining specimens

taken from the human body for the purpose

of gaining information about physiological or

pathological conditions or inborn anomalies, or

testing for safety and compatibility for poten-

tial recipients, or for monitoring therapeutic

measures.

IVD

The above-named microscopes comply with

the Council Directive 98/79/EC concerning in

vitro diagnostics.

•The manufacturer assumes no liabil-

ity for damage caused by, or any

risks arising from, using the microscope for

other purposes than those for which they are

intended or not using them within the speci-

fications of Leica Microsystems CMS GmbH.

In such cases, the Declaration of Conformity

shall be invalid.

•These (IVD) instruments are not

intended for use in the patient environ-

ment defined by DIN VDE 0100-710. Nor are

they designed to be combined with medical

Leica M125 B nameplate

instruments in accordance with EN 60601-1. If a

microscope is electrically connected to a medi-

cal instrument in accordance with 60601-1, the

requirements listed in EN 60601-1-1 shall apply.

Not suitable for examining potentially infec-

tious specimens. This type of instrument may

be operated by trained laboratory personnel

only.

Leica M165 C B nameplate

Leica M series User Manual 4

General Notes

Use in clean rooms

The Leica M series can be used in clean rooms

without any problems.

Cleaning

★Do not use any unsuitable cleaning agents,

chemicals or techniques for cleaning.

★Never use chemicals to clean colored

surfaces or accessories with rubberized

parts. This could damage the surfaces,

and products could be contaminated by

abraded particles.

★In most cases, we can provide special solu-

tions on request. Some products can be

modified, and we can offer other accesso-

ries for use in clean rooms.

★The cleaning of glass surfaces and objec-

tives in particular should be carried out

exclusively as outlined in the brochure

"Cleaning of Microscope Optics". The infor-

mation can be downloaded at:

http://www.leica-microsystems.com/prod-

ucts/

. Select your product and go to the "Down-

load" page.

★For additional information, refer to page

56.

Servicing

★Repairs may only be carried out by Leica

Microsystems-trained service technicians.

Only original Leica Microsystems spare

parts may be used.

Responsibilities of person in charge of

instrument

★Ensure that the Leica stereomicroscope

is operated, maintained and repaired by

authorized and trained personnel only.

Leica M series User Manual 5

Important Safety Notes

User Manual

This User Manual describes the special func-

tions of the individual modules of the Leica M

stereomicroscopy series and contains impor-

tant instructions for their operational safety,

maintenance, and accessories.

You can combine individual system articles

with articles from external suppliers (e.g. cold

light sources, etc.). Please read the User Manual

and the safety instructions from the supplier.

Before installing, operating or using the instru-

ments, read the user manuals listed above. In

particular, please follow all safety instructions.

To maintain the unit in its original condition

and to ensure safe operation, the user must

follow the instructions and warnings contained

in these user manuals.

We guarantee the quality of our products.

Our guarantee covers all faults in materials

and manufacture. It does not, however, cover

damage resulting from careless or improper

handling.

Leica M series User Manual 6

Symbols Used

Warning! Safety hazard!

•This symbol indicates especially impor-

tant information that is mandatory to

read and observe.

Failure to comply can cause the following:

★Hazards to personnel

★Functional disturbances or damaged

instruments

Warning of hazardous electrical voltage

This symbol indicates especially impor-

tant information that is mandatory to

read and observe.

Failure to comply can cause the following:

★Hazards to personnel

★Functional disturbances or damaged

instruments

Danger due to hot surface.

This symbol warns against touching hot

surfaces, e.g. those of light bulbs.

Important information

This symbol indicates additional infor-

mation or explanations that are intended

to provide clarity.

Explanatory notes

★This symbol within the text stands for addi-

tional information and explanations.

Figures

(1) Numbers in parentheses within the

descriptions relate to the figures and the

items within those figures.

Disposal

Notes on how to dispose on the micro-

scope, its components and expend-

ables.

China RoHS 50 year EFUP (Environ-

mentally friendly use period)

IVD labeling

IVD Instrument for in vitro diagnostics.

MM/YYYY

IVD manufacturing date,

for example 11 / 2011 for

November 2011.

Leica M series User Manual 7

Safety Instructions

Description

★The individual modules fulfill the highest

requirements for observation and docu-

mentation of Leica stereomicroscopes of

the M series.

Intended use of instrument

★Leica Microsystems microscopes are opti-

cal instruments for improving the visibility

of objects or specimens through magnifi-

cation. Accessories such as optical acces-

sories, stands, illumination, cameras etc.

supplement the equipment configuration.

Non-intended use

★Using the instrument in any way contrary

to the specifications in the User Manual can

lead to bodily harm and damage to objects.

Never use microscopes for in vivo examina-

tions or eye surgery if they are not expressly

intended for such use. Never install any

other plug or unscrew optical systems

and mechanical parts unless expressly

instructed to do so in the instructions.

The instruments and accessories described in

this User Manual have been tested for safety and

potential hazards. The responsible Leica affiliate

must be consulted whenever the instrument is

altered, modified or used in conjunction with

non-Leica components that are outside of the

scope of this manual!

Unauthorized alterations to the instrument or

noncompliant use shall void all rights to any

warranty claims as well as product liability and

the Declaration of Conformity.

Place of use

★Only use the instruments in closed, dust

free rooms and between +10 °C and

+40 °C. Protect the devices from oil, chemi-

cals and extreme humidity. If using the

devices outdoors, protect them from dust

and moisture. Never use electrical devices

outdoors.

★Electrical components must be placed at

least 10 cm away from the wall and away

from flammable substances.

★Avoid large temperature fluctuations,

direct sunlight and vibrations. These condi-

tions can distort micrographic images, for

example.

★In warm and warm-damp climatic zones,

the individual components require special

care in order to prevent the build-up of

fungus.

Leica M series User Manual 8

Safety Instructions (continued)

Responsibilities of person in charge of

instrument

★These Safety Instructions must be available

at the workplace.

Ensure that:

★The M series stereomicroscopes and

accessories are operated, maintained and

repaired by authorized and trained person-

nel only.

★All operators have read, understood and

observe this User Manual, and particularly

the safety regulations.

Repairs, service work

★Repairs may only be carried out by Leica

Microsystems-trained service technicians.

★Only original Leica Microsystems spare

parts may be used.

★Before opening the instruments, switch off

the power and unplug the power cable.

★Avoid contact with powered electrical

circuits, which can lead to injury.

Transport

★Use the original packaging for shipping or

transporting the individual modules of the

Leica M stereomicroscopy series and the

accessory components.

★In order to prevent damage from vibrations,

disassemble all moving parts that (accord-

ing to the user manual) can be assembled

and disassembled by the customer and

pack them separately.

Integration in third-party products

★When installing Leica products into third-

party products, the manufacturer of the

complete system or its dealer is responsible

for following all applicable safety instruc-

tions, laws and guidelines.

Disposal

★Once the product has reached the end of its

service life, please contact Leica Service or

Sales about disposal.

★Please observe and comply with the

national and federal laws and regulations

that are equivalent to EC directives such as

WEEE.

Like all electronic devices, the micro-

scope, its accessory components and

consumables must never be disposed of with

general household waste.

Leica M series User Manual 9

Safety Instructions (continued)

Legal regulations

★Observe the generally applicable statu-

tory and country-specific regulations for

accident prevention and environmental

protection.

EC Declaration of Conformity

★Electrically operated accessories are

constructed based on the state of the art

of technology and are provided with an EC

Declaration of Conformity. See page 55

Health risks

Workplaces with stereomicroscopes facilitate

and improve the viewing task, but they also

impose high demands on the eyes and holding

muscles of the user. Depending on the dura-

tion of uninterrupted work, asthenopia and

musculoskeletal problems may occur. For this

reason, appropriate measures for reduction of

the workload must be taken:

Optimal arrangement of workplace, work

assignments and work flow (changing tasks

frequently). Thorough training of the person-

nel, giving consideration to ergonomic and

organizational aspects.

The ergonomic design and construction of the

Leica M stereomicroscopy series are intended to

reduce the exertion of the user to a minimum.

Direct contact with eyepieces can be a potential

transmission path for bacterial and viral infec-

tions of the eye.

The risk can be kept to a minimum by using

personal eyepieces for each individual or

detachable eyecups.

Careful handling

★Exercise particular care when setting up

the instruments. If it is specified that two or

more people are required for setup, compli-

ance with this is mandatory.

★Never spill any liquids on electrical instru-

ments. This could cause the stereomicro-

scope and other equipment to become

electrically live and damage people and

instruments.

★Never clean instruments using corro-

sive cleaning agents or those containing

acetone. For detailed information about

care, refer to the User Manual for the instru-

ment.

★Check the power cables regularly. Defective

power cables can cause injuries.

★Wait for bulbs to cool off before changing

them. Touching hot bulbs can cause burns.

Leica M series User Manual 10

Safety Instructions (continued)

Light sources: safety regulations

★Light sources pose a potential risk from

glare. Therefore, lamps have to be operated

in closed housings and in installed condi-

tion.

★Never look directly into the beam path

(blinding hazard).

★Do not select a white, strongly reflective

background for the specimen.

External power supply for the focusing

column

(Leica M165 C B)

Permitted power supply:

SINPRO SPU46-110

Specifications:

Input: 100-240 V AC

47-63 Hz

1.2 A

Output: 33 V DC

1.51 A

For indoor use only.

External power supply for TL5000 Ergo

Permitted power supply:

SINPRO SPU130-110

Specifications:

Input: 100-240 V AC

47-63 Hz

3.2 A

Output: 33 V DC

3.93 A

max. 130 W

For indoor use only.

•Use only the power supply specified

above. Other power supplies must not

be used. If the original power supply fails or

is damaged, it must be replaced. Repair is not

permitted. Original power supplies are avail-

able from your Leica branch office or Leica

dealer.

Leica M series User Manual 11

Contents

Quick Start Guide

The Fastest Route to Success 29

Overview of an M Series Microscope 30

The correct interpupillary distance 31

Using the Eyepieces 32

Focusing 33

Adjusting the Resistance of the Focus Drive 34

Changing the Magnification (Zoom) 35

Click Stops and Magnifications 36

Parfocality: More Comfort and Convenience for Your Work 37

Iris Diaphragm 38

Eyepieces

Magnification Factor of the Eyepieces 40

Health Notes 41

Dioptric Correction 42

Dioptric Correction and Parfocality 43

Graticules 44

Objectives and

Copyright 2

Function of the Microscopes 3

General Notes 4

Important Safety Notes 5

Symbols Used 6

Safety Instructions 7

The Leica M Series

Congratulations! 14

A Step Towards Infinity 15

The Electronics: Comfort, Convenience and Safety for your Experiments

16

Maximum Compatibility 17

On We Go 18

Assembly

Base and Focusing Column 20

Optics carrier 21

Tube 22

Eyepieces 23

Objective 24

TL5000 Ergo transmitted light base 25

Wiring: Connections (Leica M165 C B) 26

Cables: Cable Duct 27

Leica M series User Manual 12

Optical Accessories

The Different Types of Objectives 46

Bases

Leica TL5000 Ergo Transmitted Light Base: Controls 48

Leica TL5000 Ergo Transmitted Light Base: Operation 49

Technical Data

Leica TL5000 Ergo Transmitted Light Base 52

Appendix

Calculating the Total Magnification and Field of View Diameter 54

Care, Maintenance, Contact Persons 55

Leica M series User Manual 13

The Leica M Series

Leica M series User Manual 14

Congratulations!

Congratulations on obtaining your new Leica M series stereomicroscope.

We are convinced it will exceed your expectations, as never before have

we applied our decades of experience in the areas of optics, mechanical

engineering and ergonomics in such an uncompromising manner.

The Leica M series embodies all the qualities you associate with the name

Leica Microsystems: excellent objectives, high-quality engineering, and

reliability. Furthermore, the modular design ensures that the M series

adapts perfectly to your needs—no matter which accessories you require

for your tasks.

Though the reliability and robustness of Leica stereomicroscopes is

legendary, like any high-tech product, the Leica M series requires a certain

degree of care and attention. Therefore, we recommend that you read

this manual. It contains all the information you need regarding operation,

safety and maintenance. Simply observing a few guidelines will ensure

that even after years of intensive use, your stereomicroscope will continue

to work as smoothly and reliably as on the very first day.

We wish you the best of success in your work— after all, you are now

equipped with the best tool!

Leica M series User Manual 15

A Step Towards Infinity

Ever since their introduction by Horatio S. Gree-

nough, stereomicroscopes have worked accord-

ing to the optical principles based primarily

on Ernst Abbe's research. For over a century,

ingenious optics designers and engineers have

worked to push magnification, resolution and

image fidelity to the limit permitted by optics.

In doing so, they have always been constrained

by the interrelation between three factors: the

higher a microscope's resolution, the lower the

available working distance. If one increases the

distance of the optical axes, the three-dimen-

sional image seen by the observer becomes

distorted— a sphere becomes an ellipse, a flat

surface curves towards the observer.

Leica M series User Manual 16

The Electronics: Comfort, Convenience and Safety for your Experiments

Never before have electronics been used as

extensively in a Leica series as in the new M

series lineup. Optics carrier, tripod, base and

illuminator are all connected using electrical

contacts—which provides a number of advan-

tages.

Fewer cables

A large part of the cables have been routed in

the interior of the column. Data are also trans-

ferred via the interface between the column,

the optics carrier and the carrier. The direct

result is that you need fewer cables—this not

only makes your workstation neater and more

comfortable, it even makes it appear larger.

Contacts not only transmit data, but also supply

the power.

Leica M series User Manual 17

Leica engineers were careful to ensure that the

new Leica M series—like its predecessors—

remains compatible with existing series. This

means that objectives, bases, tubes and so on

can be reused.

Objectives

All new objectives of the Leica M series are

parfocal.

If you prefer, you can continue to use the previ-

ous Leica objective series. In this case, parfocal-

ity is no longer guaranteed.

Tubes

The interface between the optics carrier and

the tube has remained the same, so exist-

ing tubes fit the new M series. The new tubes

are designed for eyepieces with field number

23, while the predecessor models were only

designed for field number 21, resulting in a

smaller object field.

Eyepieces

The new M series eyepieces have an audible

and tangible click to provide immediate feed-

back in case of accidental adjustment.

Maximum Compatibility

Leica M series User Manual 18

On We Go

If your new Leica microscope has already

been assembled and commissioned by your

Leica consultant, click here to skip through

the installation instructions and go directly

to the Quick Start Guide on page 28.

If, on the other hand, you are assembling the

microscope yourself, continue with the "Assem-

bly" chapter, which begins on page 19.

Leica M series User Manual 19

Assembly

Leica M series User Manual 20

The first step is to connect the focusing column

of the M series to the corresponding base.

Tools used

★Allen key, 3 mm

Assembling the column adapter

1. Securely install the column adapter on the

column using the four included screws.

Assembling the focusing drive

2. Securely screw the focusing column to the

base using the six included screws.

Base and Focusing Column

Leica M series User Manual 21

Tools used

★Allen key, 4 mm

Assembling the Optics Carrier

1. Place the optics carrier on the focus-

ing column so that the screw fits into the

thread provided and the lug fits into the

groove.

2. Press the optics carrier backwards to the

focusing column and screw it in place using

your other hand.

Optics carrier

Leica M series User Manual 22

Tube

All intermediate tubes that fit between the

optics carrier and the binocular tube are fitted

in the same manner.

Tools used

★No tools required.

Preparations

1. Unscrew the positioning screw and remove

the protective cover.

Assembling the tube

2. Push the tube (for example, the inclined

binocular tube) into the dovetail ring and

rotate it slightly in both directions until the

positioning screw meshes with the guide

groove.

3. While holding the tube only slightly, care-

fully tighten the positioning screw. It is

automatically brought to the correct posi-

tion.

Leica M series User Manual 23

Eyepieces

Tools used

★No tools required.

Magnification range

You can extend the overall magnification range

using available 10×, 16×, 25× and 40× wide-

field eyepieces for persons wearing glasses.

Preparation

1. If you want to use an optional graticule,

insert it now (page 44).

2. Remove the plastic tube guard.

Inserting the eyepieces

3. Push the eyepieces into the tubes as far as

they will go and check to ensure that they

fit tightly and accurately.

4. Securely tighten the clamping screws.

Leica M series User Manual 24

Objective

Tools used

★No tools required.

Preparation

1. Remove the protective cap on the optics

carrier by turning it.

Attaching the objective

•Hold the objective firmly during assem-

bly and disassembly so that it does not

fall onto the stage plate. This applies particu-

larly to the 2x planapochromatic objective,

which is very heavy. Remove all specimens from

the stage plate first.

2. Screw the objective clockwise into the

optics carrier.

Leica M series User Manual 25

TL5000 Ergo transmitted light base

Unpacking the base

The base is delivered with the adapter plate

installed. Make sure the instruments are

unpacked on a flat, sufficiently dimensioned,

and non-slip surface.

Refer also to the separately provided

User Manual for the TL5000 Ergo trans-

mitted light base.

Focusing drive and column

1. Unscrew the extension plate from the base

using the Allen key provided.

2. Attach your focusing drive column to the

bottom using the 4 Allen screws.

3. Reattach the adapter plate to its original

position using the 6 Allen screws.

Leica M series User Manual 26

The new Leica M series features extensive

encoding with which various microscope data

and settings can be read out and reproduced

later.

★The interface to the optics carrier is on the

column.

The terminals The connection to other instruments is made

using the terminals on the rear side of the

column:

1. 2×CTL2 terminals for auxiliary equipment

such as the ring illuminator, the base and

other accessories from the Leica product

range.

2. Terminal for the 50-watt power supply

provided.

Wiring: Connections (Leica M165 C B)

1

2

Leica M series User Manual 27

The integrated cable duct in the column

enables a neat cable layout around the micro-

scope. For example, the USB or FireWire cables

of the camera can be stowed in the cable duct.

Feeding the cables

1. Unscrew the three screws on the cable

duct.

2. Remove the cover of the cable duct.

3. Place the cables in the cable duct and screw

the cover on tightly.

Tip: Estimate the length of the cable ends you

will need before screwing on the cover.

For thick cables, it is difficult to change the

length retroactively.

Cables: Cable Duct

Leica M series User Manual 28

Quick Start Guide

Leica M series User Manual 29

The Fastest Route to Success

Your Leica stereomicroscope has been delivered in completely assembled

condition by your Leica partner, and naturally you want to get right to

work. Therefore, your next step should be to study the Quick Start Guide,

which outlines the most important steps at a glance.

This manual will then familiarize you with the finer details of your micro-

scope. The following pages contain important, practical information that

makes using it every day easier.

Take time to read it – it's worth it!

The M series Quick Start Guide (included with instrument).

Leica M series User Manual 30

Overview of an M Series Microscope

1 Switch click stops on/off

2 Magnification changer (zoom)

3 Coarse/fine focusing

4 Transmitted light base

5 Interchangeable objective

6 Built-in iris diaphragm

7 Fastening screw for the binocular tube (or accessories)

8 Adjustable eyepiece tubes

9 Fastening screws for the eyepieces

10 Eyepieces for spectacle wearers with dioptric correction

and eyecups

11 Trinocular tube

10

7

8

9

6

5

3

1

2

4

11

Leica M series User Manual 31

The correct interpupillary distance

The interpupillary distance is correctly set if you

see a single circular image field when looking

at a specimen.

If you are still a novice microscope user, you

may need a short time to become accustomed

to this. Not to worry—after a little while, it will

become automatic.

Reference values

The distance between eye and eyepiece

measures approx. 22 mm for 10/23B widefield

eyepieces for persons wearing glasses.

Adjusting the interpupillary distance

1. Look into the eyepieces.

2. Hold the eyepieces with both hands. Push

the eyepieces together or separate them

until you see a circular image.

3. Slowly approach the eyepieces with your

eyes until you can see the complete image

field without corner cutting.

✓

✗

Leica M series User Manual 32

If you wear glasses:

1. Hold the eyepiece firmly and rotate the

eyecups counterclockwise towards the rear,

as otherwise the viewing distance is too

great.

2. If an eyepiece is equipped with the inte-

grated dioptric correction, turn the value to

the "0" mark.

By the way, one benefit of viewing with

eyeglasses is a drastically lower risk of

bacterial transmission (see page 41).

The soft material of the eyecup also ensures

that your glasses will not be scratched, even if

they contact the eyepiece.

Using the Eyepieces

If you do not wear glasses:

1. Hold the eyepiece firmly and rotate the

eyecups forwards counterclockwise.

2. If an eyepiece is equipped with the inte-

grated dioptric correction, turn the value to

the "0" mark.

The eyepieces form the connection between

the tube and the eye of the observer. Simply

push them into the tube and they are ready to

use.

Each eyepiece offers a certain magnification

factor that has a determinative effect on the

total magnification. Furthermore, all Leica

eyepieces can be equipped with practical grati-

cules that enable measuring and quantifying of

specimens.

Dioptric Correction

A built-in dioptric correction is available for

eyeglass wearers. For more information, refer to

page 42

Leica M series User Manual 33

Focusing

Focusing raises or lowers the stereomicroscope

using the focusing drive. The specimen detail is

brought into sharp focus as soon as it is in the

focal point of the objective.

★The focusing drive can be operated either

left- or right-handed.

Focusing

★The inner, coarse adjustment is used for

covering great distances.

★The outer, fine adjustment is used for fine

focusing.

Coarse/fine adjustment

The sharpness is adjusted using the coarse/fine

adjustment.

•2. The coarse/fine adjustment carries a

load of up to 15 kg.

★The resolution of the coarse/fine adjust-

ment is 1 µm.

Leica M series User Manual 34

Adjusting the Resistance of the Focus Drive

Adjusting the resistance

Is the focusing drive too loose or too tight?

Does the equipment tend to slide downwards?

The resistance can be adjusted individually

depending on the equipment weight and

personal preferences as follows:

1. Grip the outer drive knobs with both hands

and turn them towards each other until the

desired resistance is reached during focus-

ing.

Leica M series User Manual 35

Changing the Magnification (Zoom)

All M series microscopes have an integrated

zoom. The name indicates the zoom range

covered:

★Leica M125 B = 12.5:1

★Leica M165 C B = 16.5:1

The rotary knob for the zoom can be used either

left or right-handed.

Zooming

1. Look into the eyepieces.

2. Focus on the specimen.

3. Rotate the magnification changer until the

desired magnification is configured.

Leica M series User Manual 36

The zoom button can optionally be operated

either with or without ratchet steps. Continu-

ous zoom is possible when the click stops are

disabled, which many users find convenient.

On the other hand, when the click stops are

enabled, photographs, measurement results

etc. can be reproduced more accurately.

Enabling and disabling click stops

1. Push the top button downwards to disable

the ratchet steps.

2. Push the bottom button upwards to enable

the ratchet steps.

Magnifications and fields of view

The formula on page 54 provides additional

information about the magnifications and field

of view diameters, with consideration given to

the position of the magnification changer and

the eyepiece and objective combination used.

Click Stops and Magnifications

Leica M series User Manual 37

Parfocality: More Comfort and Convenience for Your Work

All Leica stereomicroscopes are parfocally

matched, meaning that you can view a focused

specimen from the lowest to the highest

magnification without having to refocus. The

focus needs to be readjusted only if you want

to view a specimen detail that is located higher

or lower.

Requirements for parfocal work

★If you are using an eyepiece with dioptric

correction, the procedure differs from this

description. For more information, refer to

page 43

Parfocality

1. Enlarge the view to the maximum level.

2. Focus on the specimen.

You are done! Even if you select a smaller work-

ing distance, the specimen remains pin-sharp.

The parfocality is maintained until you focus on

another level of the specimen.

Leica M series User Manual 38

The iris diaphragm in the optics carrier of your

M series microscope has the same purpose as

those in a camera shutter: it regulates the avail-

able light, which changes the depth of field. The

"depth of field" (or "depth of focus") is the area

of a specimen that is brought into sharp focus.

Closing the iris diaphragm

★Close the iris diaphragm by turning the

knob to the left. The subject appears darker

and the depth of field increases.

Opening the Iris Diaphragm

★Open the iris diaphragm by turning the

knob to the right. The subject now appears

brighter, but the depth of field decreases.

Iris Diaphragm

Leica M series User Manual 39

Eyepieces

Leica M series User Manual 40

Magnification Factor of the Eyepieces

An eyepiece not only makes it possible to look passively into the micro-

scope, but also has a critical effect on the maximum magnification. The

magnification factor is between 10x and 40x.

The following eyepieces are available for the M series:

Magnification Dioptric Correction Order number

10× ± 5 diopter settings 10 450 023

16× ± 5 diopter settings 10 450 024

25× ± 5 diopter settings 10 450 025

40× ± 5 diopter settings 10 450 026

Leica M series User Manual 41

Health Notes

Potential sources of infection

•Direct contact with eyepieces is a

potential transmission method for

bacterial and viral infections of the eye. The risk

can be kept to a minimum by using individual

eyepieces or detachable eyecups. Eyecups can

be ordered separately. Please contact your

Leica partner.

Separate eyecups are an effective way of prevent-

ing infections.

Leica M series User Manual 42

All Leica eyepieces are also available with built-

in dioptric correction, allowing the microscope

to be used without glasses even by those with

vision problems. The correction comprises ±5

diopter settings.

Using the Dioptric Correction

1. Set the dioptric correction of both

eyepieces to the mid position ("0" diopter

settings).

2. While wearing your glasses, look through

the eyepieces and focus on the specimen.

3. Rotate both eyepieces to the maximum

value of "+5".

4. Hold one eye closed and rotate the other

eyepiece in the "-" direction until the speci-

men appears sharp.

5. Then, open the other eye and correct the

diopter settings until the image is uniformly

sharp.

Note that when using dioptric correction,

the advantage of parfocality is lost - thus

you have to manually refocus each time you

change the zoom level. To also use parfocality

with dioptric correction, refer to the instruc-

tions on page 43

Dioptric Correction

Leica M series User Manual 43

Adjusting

1. Set the dioptric correction for both

eyepieces to "0".

2. Select the lowest magnification and focus

on a flat specimen.

3. Select the highest magnification and read-

just the sharpness.

4. Select the lowest magnification again, but

do not look into the eyepieces.

6. Rotate the eyepieces counterclockwise in

the "+" direction as far as they will go (+5

diopter settings).

Leica stereomicroscopes are parfocally

matched. The prerequisite for this is the correct

setting of the diopters and the parfocality. The

following adjustments have to be carried out

only once by each user.

Preparations

★Move the lever of the video/phototube to

the "observation" position and open the

diaphragm.

7. Look into the eyepieces.

8. Slowly rotate each eyepiece individually

in the "–" direction until each eye sees the

object sharply imaged.

9. Select the highest magnification and refo-

cus if necessary.

Now, if you adjust the magnification from the

lowest to the highest level, the specimen is

always brought into sharp focus. If not, repeat

the process.

Dioptric Correction and Parfocality

Leica M series User Manual 44

Graticules

2. Clamp the graticule on the insert, applying

moderate pressure. Ensure that the grati-

cule fits tightly.

3. Screw the insert and graticule firmly into

place and replace the eyepiece in the tube.

4. You can now align the graticule by rotating

the eyepiece in the tube and then tighten-

ing it using the clamping screw.

Use

Leica graticules make counting easier, particu-

larly for workstations that are not equipped

with a digital camera.

The Leica graticules for length measurements

and numbering are fitted in mounts and are

inserted into the eyepieces.

1. Screw the insert off of the eyepiece.

Leica M series User Manual 45

Objectives and

Optical Accessories

Leica M series User Manual 46

The Different Types of Objectives

To meet the various requirements regarding

imaging properties, there is a choice of high-

quality interchangeable planachromatic and

planapochromatic objectives and also lower-

priced interchangeable achromatic objectives.

★Achromatic objectives are particularly

suited for specimens with high-contrast

structures.

★Flat-field (planachromatic) objectives are

particularly well suited for studying flat

objects such as wafers and thin sections.

★With planapochromatic objectives, the

finest structures are visible with high

contrast. The sophisticated apochromatic

correction allows these objectives to attain

the highest color brilliance and fidelity.

Achromatic objectives

The 0.32×, 0.5×, 0.63×, 0.8×, 1×, 1.5×, 2×

achromatic objectives offer countless variants

for selecting the object field diameter, magnifi-

cation ranges and working distances (see page

54).

Planachromatic objective 1×

For the highest requirements for overall image

quality, we recommend equipping the micro-

scope with the 1× plan (flat-field) objective,

which returns sharp, contrast-rich object fields

all the way to the border.

Achromatic objectives with a long focal

length

For special applications, achromatic objectives

with long working distances and focal lengths

of f=100 mm to 400 mm are available.

Leica M series User Manual 47

Bases

Leica M series User Manual 48

Leica TL5000 Ergo Transmitted Light Base: Controls

Refer also to the separately provided

User Manual for the TL5000 Ergo trans-

mitted light base.

1 Power switch for the base

2 Power supply connection 1 On/Off switch for the light source / Press and

hold for 5 seconds to reset the base to factory

default settings

2 Controls for controlling the brightness inten-

sity

Rear side of the TL5000 Ergo Left side of the TL5000 Ergo Right side of the TL5000 Ergo

1 LED display for opening and positioning

the aperture or for balance in DF mode.

2 Control for the size of the aperture/

balance

3 "BF" button for bright field (press and

hold for 2 seconds to (de)activate the

automatic aperture)

4 "RC" button for Rottermann Contrast / in

the RC mode, press and hold the button

to define the aperture size

5 "DF" button for dark field

1 12 23 4 5

12

Leica M series User Manual 49

2. Press the on/off switch on the right side

once to turn on the illumination.

Switching the transmitted light base on

and off

1. Switch on the transmitted light base with

the power switch on the rear side.

Leica TL5000 Ergo Transmitted Light Base: Operation

The transmitted light base must only be

connected to a grounded socket with a

faultless power cable! Failure to observe these

warnings may result in serious personal injury

or even death!

•The LED illumination can be very bright!

Check and adjust the intensity of the

illumination to a suitable brightness before

looking through the eyepieces.

Continued on next page.

Leica M series User Manual 50

4 Press the on/off switch on the right side

once again to turn off the illumination on

the base.

Leica TL5000 Ergo Transmitted Light Base: Operation (Continued)

3. Look through the eyepiece and adjust the

light intensity using the control on the

right side. The intensity of the illumination

is visualized by the LED scale.

The following assumes that the power

switch on the rear side of the device

is always switched on. This switch is not

mentioned in the remaining part of this User

Manual.

Leica M series User Manual 51

Technical Data

Leica M series User Manual 52

Leica TL5000 Ergo Transmitted Light Base

Light source

Light source LED

Illuminated field: Bright field ∅65 mm

Illuminated field: Dark field ∅40 mm

Relief Contrast System (RC™) Yes

Internal shutter/lamp control Yes

Integrated filter holder Yes

Matching of high num. aperture Yes

Remote control options Yes

AntiShock™ Pads Yes

Dimensions (W×H×D) 412×341×46 mm

Power supply

Input 100–240 VAC 47 – 63 Hz 3.2 A

Output 33 VDC 3.93 A 130 W MAX

Connections

Power supply 1

Leica M series User Manual 53

Appendix

Leica M series User Manual 54

Calculating the Total Magnification and Field of View Diameter

Parameter

MO Magnification of objective

ME Magnification of eyepiece

zMagnification changer position

qTube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45°

ErgoTube™

rFactor 1.25× if the planachromatic and planapochromatic

objectives of the MZ125/MZ16 are used on the MS5, MZ6,

MZ75 or MZ95

NFOV Field number of the eyepiece. Field numbers are printed on the

eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.

Example

MO 1× objective

ME 25×/9.5 eyepiece

zZoom position 4

qCoaxial incident light 1.5×, tube factor

rFactor 1.25×

Calculation example: magnification in the binocular tube

MTOT VIS = MO × ME × z × q × r

or

1 × 25 × 4 × 1.5 × 1.25 = 187.5×

Calculation example: field of view diameter in the specimen

∅ OF:

NFOV

MO × z ×q ×r

Leica M series User Manual 55

Care, Maintenance, Contact Persons

We hope you enjoy using your high-perfor-

mance microscope. Leica microscopes are

renowned for their robustness and long service

life. Observing the following care and clean-

ing tips will ensure that even after years and

decades, your Leica microscope will continue to

work as well as it did on the very first day.

Warranty benefits

The guarantee covers all faults in materials

and manufacture. It does not, however, cover

damage resulting from careless or improper

handling.

EC Declaration of Conformity

To download the EC Declaration of Conformity,

use this link

http://www.leica-microsystems.com/

products/stereo-microscopes-macroscopes/

research-manual/

Select the microscope type and go to the

"Download" page.

Care

★Protect your microscope from moisture,

fumes and acids and from alkaline, caustic

and corrosive materials and keep chemicals

away from the instruments.

★Plugs, optical systems and mechanical parts

must not be disassembled or replaced,

unless doing so is specifically permitted

and described in this manual.

★Protect your microscope from oil and

grease.

★Do not grease guide surfaces or mechani-

cal parts.

Leica M series User Manual 56

Care, Maintenance, Contact Persons (continued)

Protection from dirt

Dust and dirt will affect the quality of your

results.

★Put a dust cover over the microscope when

it will not be used for a long time.

★Use dust caps to protect tube openings,

tubes without eyepieces, and eyepieces.

★Keep accessories in a dust-free place when

not in use.

Cleaning polymer components

Some components are made of polymer or are

polymer-coated. They are, therefore, pleasant

and convenient to handle. The use of unsuit-

able cleaning agents and techniques can

damage polymers.

Permitted measures

★Clean the microscope (or parts of it)

using warm soapy water, then wipe using

distilled water.

★For stubborn dirt, you can also use ethanol

(industrial alcohol) or isopropanol. When

doing so, follow the corresponding safety

regulations.

★Remove dust with a bellows and a soft

paintbrush.

★Clean eyepieces and objectives with special

optics cleaning cloths and pure alcohol.

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