Defiant FROG 5000 Chemical Analysis System User’s Manual

User Manual: Manual

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FROG-5000TM
Chemical Analysis System

User’s Manual
Defiant Technologies, Inc.
2018 Rev.3

How to assemble a ferrule.
This problem has arisen so often and caused so many
problems, that we have added it as a preface to the manual. There is a Teflon ferrule where the air sampler and
glass sparge bottle mounts to the FROG-5000TM. At some
point, the ferrule will probably be disassembled and if it
is re-assembled incorrectly, there will be a leak in the system. The photograph below shows the proper orientation of the ferrule components:

We hope that this helps you
achieve the full degree of enjoyment our instrument can
provide.
Sincerely,

Defiant Tech

FROG-5000TM
Chemical Analysis System
User’s Manual

Defiant Technologies, Inc.
2018 Rev. 3
www.defiant-tech.com

FROG-5000TM User’s Manual

Sparge Bottle

FROG-5000TM User’s Manual

HANDLING INSTRUCTIONS
FROG-5000TM is a robust, portable instrument, which can be
operated in a lab setting or in the field. This instrument can be
damaged if the unit is mishandled. The following precautions are
given to ensure correct handling of the instrument.

PRECAUTIONS
•

DO NOT invert with water in the sparge bottle.
System can NOT analyze a water or soil sample when
inverted!
Damage to instrument is possible!
(If this does occur, the system may require maintenance. Refer to
Section 8, Trouble Shooting)

•

DO NOT handle or carry system when water sample is being
analyzed. For the best results, limit movement to before or
after sample analysis.

•

DO NOT transport or store the instrument with liquid in the
sparge bottle.

When there is Water in the Sparge Bottle
Do NOT invert 90° Angle

Do NOT invert 180° Angle

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FROG-5000TM User’s Manual

TABLE OF CONTENTS
Handling Instructions ............................................................................. 5
Commonly Used Acronyms .................................................................... 9
Section 1: Getting to Know the FROG-5000TM........................................ 11
1.1 Manual Overview ......................................................................... 11
1.2 Operations Overview ................................................................... 12
Section 2: Components and Systems ..................................................... 15
2.1 Instrument Components ............................................................... 16
2.2 Purge and Trap System ................................................................ 20
2.2.1 Load/Analyze Valve .............................................................. 22
2.2.2 Sparge Bottle Positions ........................................................ 23
2.3 Gas Phase Analysis System ........................................................... 24
2.4 Gas Sample Analysis Flow Diagrams ............................................ 27
2.4.1 Sample Loading .................................................................... 27
2.4.2 Sparge and Collection ........................................................... 28
2.4.3 Release, Separation and Analysis ......................................... 29

2.5 Gas Sampling Analysis Flow Diagrams Air Sampler………………..…..30
2.5.1 Sample Collection………………………………………………….………….30
2.5.2 Release, Separation and Analysis…………………….………………..31
Section 3: Overview of EllvinTM Chromatography Software ................... 32
Section 4: Field and Lab Analysis Procedures ........................................ 33
4.1 Powering On/Charging the Battery .............................................. 37
4.2 Connecting to Computer/EllvinTM ................................................. 41

4.3 Instrument Settings ...................................................................... 45
4.4 Instrument Preparation ................................................................ 49
4.4.1 Rinsing the Instrument ......................................................... 49
4.4.2 Creating a Clean Baseline ..................................................... 50
4.4.3 Connecting the Air Sample Adaptor ..................................... 53
4.5 Loading a Sample .......................................................................... 57
4.6 Running a Sample ......................................................................... 61
4.7 Importing Data to EllvinTM ............................................................ 67
7

FROG-5000TM User’s Manual

TABLE OF CONTENTS CONTINUED
Section 5: EllvinTM Chromatography Software ..................................... 73
5.1 Features of EllvinTM ..................................................................... 74
5.2 Applying EllvinTM Features .......................................................... 80
5.2.1 OPEN Button ...................................................................... 80
5.2.2 Magnifying Glass ................................................................ 82
5.2.3 Integrator ........................................................................... 84
Section 6: Calibration ........................................................................... 91

6.1 Calibration Standards ................................................................. 92
6.1.1 Standards for Liquid or Soil Samples .................................. 92
6.1.2 Standards for Air Samples .................................................. 94
6.2 EllvinTM Calibration Window Features ........................................ 97
6.2.1 List of Analytes ................................................................... 97
6.2.2 Correlation Chart and Equations ........................................ 99
6.3 Calibration Procedure ................................................................ 100
Section 7: Maintenance ...................................................................... 109

7.1 Routine Cleaning ................................................................... 109
7.2 Cleaning the Scrubber ........................................................... 110
Section 8: Trouble Shooting ................................................................. 115
8.1 Inverting the FROG with
Water in the Sparge Bottle ........................................................ 115
8.2 Degrading Chromatograph:
Scrubber is NOT secured properly ............................................. 119
8.3 Cannot Achieve a Clean Baseline ............................................... 120
8.4 Cool Features (Continuous Operation) ..................................... 122
8.5 Really Useful Features …………………………………………………………….123
9.1 Care for the Li-Po Battery ……………………………………………………….124
10.1 Repositioning the sparge nut cover ……………………………………...125
Appendix I: Accessories/Spare Parts ………………………………….……………..127
Appendix II: Detectable Chemicals ....................................................... 133

FROG-5000TM User’s Manual

COMMONLY USED ACRONYMS
PC: Preconcentrator
PID: Photo Ionization Detector
GC: Gas Chromatography
VOCs: Volatile Organic Compounds
BTEX: Benzene, Toluene, Ethylbenzene, Xylenes
EllvinTM: Defiant’s Chromatography Software

FROG-5000TM

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FROG-5000TM User’s Manual

SECTION 1: GETTING TO KNOW THE FROG-5000 T M
1.1 MANUAL OVERVIEW
The FROG-5000TM uses a practical approach to instrument set-up
and operations. This manual includes the following:
• An overview of the FROG-5000

operational systems,

components, and features.
• Diagrams illustrating the flow of a sample during loading,

sparge, collection and analysis.
TM

• Instructions for using the chromatography software (Ellvin ).
TM

• Detailed procedures for using the FROG-5000 .
• Instructions for calibrating the instrument.
• Routine maintenance information.
• Troubleshooting information.

FROG-5000TM User’s Manual

1.2 OPERATIONS OVERVIEW
The FROG-5000TM instrument analyzes air, water or soil to identify
volatile organic compounds (VOCs) present in a sample and
determines their concentrations.
The instrument is designed to operate either in the field or in a
laboratory, and either connected or not connected to a computer
running the EllvinTM Software. Data is stored on the user’s hard
drive when the FROG is attached to a computer. Data is also
stored on the FROG’s internal memory card and can be imported
to a computer later.
The FROG-5000TM has a 5 mL sparge bottle that can be used for
water or solid samples. It is good practice to keep the total
volume in the sparge bottle at a constant 5 mL. For instance, if
the user wishes to dilute a water sample 10 to 1, a sample
volume of 0.5 mL would be diluted with 4.5 mL of clean water,
resulting in a total volume of 5 mL.
If analyzing soil samples, the user must add water to the sparger
to ensure that the sample is appropriately agitated.
The basic steps for operating the system are:
1. Adjust/apply the settings as needed.
2. Ensure that the instrument displays a clean baseline.
3. Load a 5 mL sample.
4. Run an analysis, either connected or not connected to
EllvinTM.
5. If not connected to Ellvin during the analysis cycle, read
analysis data using Ellvin at a later time.
NOTE: A working knowledge of EllvinTM is necessary to correctly
operate the instrument. Users should be familiar with the
software before attempting to analyze samples.
12

FROG-5000TM User’s Manual

The FROG-5000TM is designed for use in the field.

The FROG-5000TM is designed for use in the laboratory. Connect to
EllvinTM Software for more detailed analysis results.

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FROG-5000TM User’s Manual

SECTION 2: COMPENENTS AND SYSTEMS
The FROG-5000TM instrument has two main operational systems:
The Purge System
This system manages the loading of a sample, sparging the
sample, if liquid, to release VOCs, and the introduction of gas
phase compounds to the Gas Phase Analysis System. All
components of the Purge System are visible on the instrument
exterior.
The Gas Phase Analysis System
The Gas Phase Analysis System receives the VOCs from the Purge
System and carries them through all stages of sample analysis,
Collection Injection Separation Detection
The instrument’s components are briefly described on the
following pages.

FROG-5000TM User’s Manual

Section 2: Components and Systems Continued

2.1. INSTRUMENT COMPONENTS
Components Reference for FROG-5000TM:
A. Scrubber
B. Load/Analyze Valve Handle
C. Display Screen
D. Sparge-Air Line
E. Sparge-Valve
F. Flow Selection-Valve
G. Sparge Manifold
H. Pump Vent Orifice
I.

Pump Air Supply Inlet

Sample Inlet

K. Sparge Bottle with PEEK Tube (i.e., sparge needle)
L. Sparge Bottle Nut
M. 5 mL Syringe to load water sample

FROG-5000TM User’s Manual

E
D
A

I
F
L

FROG-5000TM User’s Manual

2.1. Instrument Components Continued
N. micro-SD Card for stored chromatograms
O. Run Start and Data Toggle Switch
P. Port for Serial Data Plug
Q. Port for 12VDC Power Plug
R. Port for Pump Intake for Carrier/Sparge Gas
S. Battery Cover

T. Power Port for Air Sampler Adapter
U. Exhaust Port from Gas Analysis Module
V. Power Switch
W. Battery Charging Light
X. Battery Charge Level Indicator

FROG-5000TM User’s Manual

S
O

FROG-5000TM User’s Manual

Section 2: Systems and Components Continued

2.2. PURGE SYSTEM
Purge System Components and their Functions
Figures 2.2-1 and 2.2-2.
A) Scrubber: Activated Carbon and Molecular Sieves
Function: Cleans the ambient air to prevent external VOCs
from interfering in analyses.
*See NOTE on following pages for replacement supplies.
B) Load/Analyze Valve:
Function: Opens and closes the sample inlet for sample
loading. The valve has three positions, LOAD WATER, RUN
WATER and RUN AIR.
E) Sparge-Valve:
Function: Directs pumped air from purging water to analysis.
G) Sparge Manifold:
Function: Routes gas to the proper ports for sparging and
analysis functions.
J) Water Sample Inlet:
Function: Attachment for syringe to load a sample.
K) Sparge Bottle with PEEK Tube: Needle type sparge bottle,
0.5” x 5mL.
Function: Holds sample to purge VOCs. The Sparge bottle has
two positions, UP and DOWN.
(See Section 2.2.2 for operating instructions)
L) Sparge Bottle Nut:
Function: Holds Sparge bottle in place and provides gas tight
seal.
M) Syringe: 5mL, with Luer Lock.
Function: Loads a fixed sample volume to the sparger.
Attaches to sample inlet (J).

FROG-5000TM User’s Manual

Figure 2.2-1: Purge and Trap System
B
A

J
G

E
L
K

Figure 2.2-2: Purge System

J
M

FROG-5000TM User’s Manual

2.2 Purge and Trap System Continued

2.2.1. LOAD/ANALYZE VALVE
• Turn the load/analyze valve to

the LOAD WATER position to
load a water sample.

LOAD WATER
POSITION
•

After loading a water
sample, turn the handle
counter clockwise to the
RUN WATER position before
starting a water analysis
cycle.

•

When running air samples,
leave the load/analyze valve
in the RUN AIR position

COLLECT/ANALYZE
AIR POSITION

FROG-5000TM User’s Manual

2.2.2. SPARGE BOTTLE POSITIONS
The sparge bottle moves UP and DOWN and is secured with a
sparge bottle nut.
•

The bottle must be in the DOWN position to rinse the
instrument or to remove the sparge bottle.

•

The bottle must be in the UP position to analyze a sample.

•

The bottle may be in the UP or DOWN position for sample

loading. We will present the DOWN position for consistency
in this manual.

Sparge bottle
in the UP position.

Sparge bottle
in the DOWN position.

NOTE: When the sparge bottle is fully UP, the sparge tube is
near the bottom of the sparge bottle. A partially lowered
sparge bottle will cause analyte peaks to shift right.

FROG-5000TM User’s Manual

Section 2: Components and Systems Continued

2.3. GAS PHASE ANALYSIS SYSTEM
Gas Phase Analysis System Components and their Functions
Figures 2.3-1 and 2.3-2:
A-Internal) Pump: Diaphragm pump
Function: Circulates carrier gas through system.

B-Internal) Preconcentrator (PC):
Function: Collects and injects VOCs into GC column.
C-Internal) Micro Gas Chromatography Column: (GC)
Function: Separates VOCs.
D-Internal) Photo Ionization Detector (PID): 10.6eV lamp
Function: Detects VOCs.
E) Sparge-Valve:
Function: Directs pump flow into sparge bottle to purge
VOCs from sample.
F -Internal) Bypass-Valve:
Function: Allows the VOCs to pass over the PC and bypass
the GC and PID.
G) Pump Vent Orifice:
Function: Provides a small leak in pump flow for improved
flow control.
H-Internal) Injection Split Vent:
Function: Provides a small leak to prevent rebound injection.

FROG-5000TM User’s Manual

A
F

D
C
B

H
E

Figure 2.3-1 Gas Phase Analysis System components.

PID

Preconcentrator

Bypass-Valve

micro GC Column
Figure 2.3-2 Gas Phase Analysis System internal components.

FROG-5000TM User’s Manual

2.3. Gas Phase Analysis System Continued
Gas Phase Analysis System Components and their Functions
Figure 2.3-3
Gas/Purge System, An Internal View. Analytes that are
sparged from water samples exit through a port at the top
of the sparge bottle and move through the transfer line to
the Gas Analysis System

SPARGE
VALVE
SPARGE
BOTTLE
EXIT

AIR BLEED
ORIFICE

GAS
TRANSFER
LINE

BYPASS
LINE
BYPASS
VALVE

PID

SPLIT
VENT

PUMP

Figure 2.3-3 Gas Phase Analysis System internal and external
components

FROG-5000TM User’s Manual

Section 2: Systems and Components Continued

2.4. GAS FLOW DIAGRAMS
This section describes and illustrates the flow of a sample from
loading through the components of the Gas Phase Analysis
System (Section 2.2).

2.4.1. SAMPLE LOADING
(Diagram 2.4-1) The sparge bottle is in the DOWN position for
loading a liquid sample. The load/analyze valve is in the LOAD
position. A sample is loaded into the sample inlet with a 5mL
syringe.

LOAD/ANALYZE VALVE

Diagram 2.4-1: Sample Loading

FROG-5000TM User’s Manual

2.4.2. SPARGE AND COLLECTION
(Diagram 2.4-2) After the sample has been loaded, the load/
analyze-valve is placed in the RUN WATER position, and the
sparge bottle is moved into the UP position. The start button is
pressed, and the pump flow starts. The carrier gas moves
through: 1) the activated charcoal/molecular sieve scrubbers
then to the 2) sparge valve. If the sample is a liquid, scrubbed
air sparges the liquid to release the VOCs into the headspace of
the sparge bottle, through the gas transfer line and onto the
preconcentrator (PC). The scrubbed air continues past the PC
and out of the system exhaust.

Diagram 2.3-2: Sparge and Collection

FROG-5000TM User’s Manual

2.4.3. INJECTION (RELEASE), SEPARATION, AND ANALYSIS
(Diagram 2.4-3) Once the VOCs are collected onto the PC, the PC
is heated to release the VOCs and inject them into the Micro-Gas
Chromatograph Column (GC). The VOCs separate through the
column and then continue to the Photo Ionization Detector
(PID). The PID signal is analyzed and displayed through the
software. The sample then exits through the exhaust.

Diagram 2.4-3: Injection, Separation and Analysis

FROG-5000TM User’s Manual

Section 2: Systems and Components Continued

2.5. GAS FLOW DIAGRAMS THROUGH AIR SAMPLER
This section describes the flow of an air sample when it is
loading into the Gas Phase Analysis System using the air sampler (see Section 4.4.3).

2.5.1. SAMPLE COLLECTION
(Diagram 2.5-1) The air sampler is connected to the FROG5000. The start button is pressed and sample collection starts.
Sample gas moves through: 1) an inert valve in the air sampler
2) over the preconcentrator, and 3) out of the gas module. A
pump in the air sample adapter provides vacuum for pulling
the air sample over the preconcentrator.

Diagram 2.5-1: Sample Collection
30

FROG-5000TM User’s Manual

2.5. Gas Flow Diagram Continued

2.5.2. INJECTION (RELEASE), SEPARATION, AND ANALYSIS
(Diagram 2.5-2) Once the VOCs are collected onto the PC, the
PC is heated to release the VOCs and inject them into the Micro
-Gas Chromatograph Column (GC). The VOCs separate through
the column and then continue to the Photo Ionization Detector

(PID). The PID signal is measured and displayed through the
software. The sample exits through the exhaust.

Diagram 2.5-2: Injection, Separation and Analysis

FROG-5000TM User’s Manual

SECTION 3: OVERVIEW OF ELLVIN™ SOFTWARE
The EllvinTM Software has four windows that are accessed with
tabs when the software is open.

Window Tabs in EllvinTM
T

• Live Data Window: This is the default window for Ellvin M. In

Live Data Window, EllvinTM creates a chromatogram from
sample analysis data. The data source may be:
a. From a sample loaded and simultaneously analyzed
by Ellvin™.
b. From sample data previously recorded on the
instrument’s SD card, then imported to Ellvin™.
• Analyze Window: In this window, Ellvin™ retrieves analysis
data that was previously stored on a computer and creates a
chromatogram. In the Analyze Window, the user can
examine chromatograms closely, integrate chromatogram
peaks, and export data to Excel.
• Calibration Window: In this window, Ellvin™ uses analysis

data from chemical standards to create a FROG calibration
file.
• Settings Window: In this window, Ellvin™ manages the

settings for operation of the FROG. The settings define
temperature and duration parameters for various phases of
the instrument’s collection and analysis cycle.
There are several general features of Ellvin™ as well as a few
features specific to Analyze Window that are described in
Section 5. The user should be familiar with all the features
before starting a sample analysis.

FROG-5000TM User’s Manual

SECTION 4: FIELD AND LAB ANALYSIS PROCEDURES
The simplest way to use the FROG is to load samples and run
analyses while the instrument is connected to a computer and
Ellvin™. The software analyzes sample data and displays a live
chromatogram during analyses. The length of the cycle depends
on the parameters set for each analysis. Data is stored both on
the FROG’s internal SD card and on the computer.

The FROG can also analyze samples when not connected to
Ellvin™. An analysis cycle takes the same amount of time as it
does when it’s connected, and an estimate of the results is
displayed on the instrument’s screen. Data is stored on the
FROG’s internal SD card and can be imported to Ellvin™ and
analyzed at a later time if desired. Section 4.7. gives instructions
for importing data from the FROG.
Instrument Display

Continued next page.
33

FROG-5000TM User’s Manual

Section 4: Field and Lab Analysis Procedures Continued
This section provides detailed instruction for various procedures
that are essential to operating the FROG-5000TM.
Procedures include:

4.1: Powering On/Charging Battery
Description of components and connections.
4.2: Connecting to Computer/EllvinTM
Instructions/description for components and connections.

4.3: Instrument Settings
Set parameters to optimize instrument function.

4.4: Instrument Preparation
Remove interfering contaminants/ensure a clean instrument.

4.5: Loading a Sample
Air, liquid, and soil sample preparation and loading instructions.
4.6: Running A Sample
Instructions/description for an analysis cycle.
4.7: Importing Data to Ellvin™
Import stored data from FROG to Ellvin for analysis.
34

FROG-5000TM User’s Manual

Complete the following procedures to run an analysis.
NOTE: The user should be familiar with EllvinTM before loading
and running an analysis. To help meet this need, some handy
Quick Start guides are provided with the system.

4.1: Powering On/Charging Battery

4.4: Instrument Preparation

4.5: Loading a Sample

4.6: Running A Sample

If not connected to Ellvin,
5.

4.7: Importing Data to Ellvin™

6. Clean the instrument when all samples have been completed
by repeating Step 2.
4.4: Instrument Preparation

Exception: Samples of the same, known analyte may be run
consecutively from low concentration to high without rinsing the
instrument and creating a clean baseline.

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FROG-5000TM User’s Manual

Section 4: Field and Lab Analysis Procedures Continued

4.1: Powering On/Charging Battery
Powering On
•

Connect power cable to power port (R) 12V POWER, or use
battery power. (Figure 4.1-1)

•

Turn power switch (O) to ON (fully up) position. The
instrument display screen will be visible.

To R, 12V POWER

R, 12V Power

To 100240VAC
Outlet

Figure 4.1-1: Power port, power cable and power switch.

•

When the FROG-5000TM display screen shows READY, the
instrument is ready to perform an analysis.

FROG-5000TM User’s Manual

4.1. Powering On/Charging Battery Continued

Charging the Battery
1. Plug 12 VDC power supply into 12VDC POWER port.
2. The battery recharges while the unit is plugged into external
power.

3. Blue light shows power is supplied.
4. Flashing green light indicates battery is charging.
5. Unit may be operated while charging.

FROG-5000TM User’s Manual

To R, 12V POWER

R, 12V Power

To 100240VAC
Outlet

Blue Light—Power Connected
Green Flash—Battery Charging

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FROG-5000TM User’s Manual

Section 4: Field and Lab Analysis Procedures Continued

4.2: Connecting to Computer/Ellvin™
There are two possible connections between the FROG and a
computer.
To receive live data and communicate with the FROG through
EllvinTM, connect a computer to the serial data port (Q) on the
FROG with the USB-to-Serial-Data Cable.

To import data from the instrument’s micro-SD card (N), press in
to eject the card. An adapter is provided to plug the card into a
USB port on a computer.

Q, Data Port

To Laptop USB

To Q for
Serial Data
USB to Serial Data-Cable

FROG-5000TM User’s Manual

4.2. Connecting to Computer/Ellvin Continued

Serial data-cable Connection
1. Connect the serial data-cable from the FROG’s serial data
port (Q) to a USB port on a computer.
2. Set the instrument power switch to the ON position.
3. If necessary, install Ellvin™ onto laptop or
PC.
4. Open Ellvin™ by double clicking on the
Ellvin™ icon.

USB

To Laptop USB

To Q
Serial Data-Cable

FROG-5000TM User’s Manual

After Ellvin™ has opened, the default screen that appears is the
chromatogram display screen in Live Data Window.
(Figure 4.2-1)
Figure 4.2-2 shows details of the display screen

Figure 4.2-1: EllvinTM default

Software Features

Tabs

Software still not communicating with FROG-5000TM

Figure 4.2-2: Ellvin default screen details.

FROG-5000TM User’s Manual

4.2. Connecting to Computer/Ellvin™ Continued
5. Click “Port” on the display screen, then select a COM#.

6. The connection status indicator in the lower left corner
should now read CONNECTED indicating that the FROG
and Ellvin™ are connected. The user may now manage
settings and run analyses from Ellvin™.
7. If Ellvin™ ever disconnects with the FROG, a red flag will
show in the lower left corner.

FROG-5000TM User’s Manual

Section 4: Field and Lab Analysis Procedures Continued

4.3: Instrument Settings
The FROG-5000TM comes with general default settings for VOCs,
which are shown in Figure 4.3-2 on the following page.
The settings determine the duration of different analysis phases,
as well as the desired temperature of the GC column. The settings
are defined and loaded to the instrument using Ellvin™. Table 4.31 on the following page describes the various settings.
The FROG-5000TM default settings are best for the analysis of
Benzene, Toluene, Ethylbenzene and Xylenes (BTEX), but the
settings can be altered for analyzing a wide variety of compounds
and concentrations.
(See Appendix I for a list of detectable chemicals).
The current operational settings are stored in the FROG-5000TM
memory and do not revert to the default settings when the
instrument is disconnected from the software or power. They are
retrieved and displayed under the Settings Window when the
instrument is connected to a computer through Ellvin™.
Instructions for adjusting the settings begin on the following
pages.

Connect to Ellvin™ to manage the settings.

FROG-5000TM User’s Manual

4.3. Instrument Settings Continued
Table 4.3.1 Description of Settings
Settings

Description

Hold time at lower GC temperature (seconds)

Ramp time from GC cold to GC hot temperature (oC)

Hold time at hot GC temperature (oC)

Initial cold GC temperature (oC)
NOTE: If used outdoors, the user must consider
ambient conditions.

Final hot GC temperature (oC)

COLLECT

Collection time of VOCs onto PC (seconds)
This corresponds to the sparge time.

CLEAN

Time cleaning PC by heating (seconds)

PRESETTLE

Time PC cools after cleaning (seconds)

SETTLE

Time allowed for pressure to stabilize before
PC FIRE (seconds) for GC analysis

FIRE

Time PC is Heated to Release VOCs (seconds)

Figure 4.3-1: Description of GC Temperature Settings.

FROG-5000TM User’s Manual

Defining and applying the settings.
1. Connect to Ellvin™. Refer to:
4.2: Connecting to Computer/Ellvin
2. Click on the Ellvin Settings Tab.

3. Type in a non-zero integer then click SET to apply the settings
to the instrument. Changes to the settings are stored on the
instrument. Settings DO NOT revert to the default parameters when power is removed or computer is disconnected.
The minimum and maximum values are listed next to the
Figure 4.3-2.
Parameter Permitted Range Current Settings Text Box for New

Figure 4.3-2: Setting Window showing Default Settings

FROG-5000TM User’s Manual

Section 4: Field and Lab Analysis Procedures Continued

Figure 4.4-1 Rinsing the Instrument Steps 1-3

FROG-5000TM User’s Manual

4.4: Instrument Preparation
Before running analyses, the instrument should be rinsed and
display a clean baseline.

4.4.1. RINSING THE INSTRUMENT

1. Place the load/analyze valve in the
LOAD WATER position.
(See Section 2.2.1)

LOAD

2. Place sparge bottle in the DOWN
Position.
(See Section 2.2.2)

3. Attach syringe with 5 mL deionized
water to FROG-5000TM.
(Figure 4.4-1, opposite page)

Figure 4.4-2: Load/
Analyze Valve

4. Load deionized water into FROG-5000TM.
5. Remove sparge bottle and empty the water into waste container.
6. Repeat steps 3-5 twice more for a total of three rinses.
7. Remove and rinse sparge bottle with deionized water.
8. Replace the sparge bottle in the DOWN position.
Proceed to demonstrate a clean baseline (following pages).

FROG-5000TM User’s Manual

4.4. Instrument Preparation Continued

4.4.2. CREATING A CLEAN BASELINE
A Clean Baseline should be achieved before analyzing a sample.
This procedure ensures that there are no contaminants in the
instrument that could effect the analysis results.
If the instrument is connected to a computer and Ellvin, the start
button and display screen are in the software. If the instrument is
not connected to Ellvin, the start button and display screen are
on the instrument.
Rinse the instrument as described in Section 4.4.1 before
attempting to achieve a clean baseline
Procedure:
1. Place load/analyze valve in the LOAD WATER position.
(See Section 2.2.1)
2. Place rinsed sparge bottle in the DOWN position.
(See Section 2.2.2)
3. Attach syringe with 5 mL deionized water to FROG-5000TM.
4. Load deionized water into FROG-5000TM
5. Place the load/analyze valve in the RUN WATER position.
6. Move the sparge bottle to the fully UP position and tighten
the sparge nut in the clockwise direction until lightly snug.
7. Remove the empty syringe

Continued following pages.
50

FROG-5000TM User’s Manual

1
LOAD
POSITION

5
4

FROG-5000TM User’s Manual

4.4.2. CREATING A CLEAN BASELINE CONTINUED
7. Begin an analysis cycle using deionized water. If connected to
Ellvin™, click the start button in the software. If not connected to
Ellvin™, press in the top of the black toggle button on the
instrument. When the analysis is finished, a clean baseline should
appear. A clean baseline is basically smooth with no significant
peaks. A small peak at the beginning of the chromatogram and
small waves in the clean baseline are acceptable.

Start Button PRESS
AT TOP
Start Button in Ellvin™

Clean Baseline on Ellvin™ Screen

A small peak at the beginning of the chromatogram is
normal.

If Peaks do appear, repeat the instrument preparation procedures
(4.4.1, Rinsing the Instrument and 4.4.2, Creating a Clean Baseline) until a clean baseline is achieved.

FROG-5000TM User’s Manual

4.4. Instrument Preparation Continued

4.4.3. CONNECTING THE AIR SAMPLE ADAPTOR
If analyzing air samples, the user must first connect the air
sample adaptor to the FROG.

Exhaust Port Link
Power/Control
Connector

Wand Connection

Air Sampling Bag Adapter
Charcoal Trap Air Scrubber
Sample Wand

The air sample kit includes a sample wand and an air sample
bag adapter. The air sample bag adapter enables direct
connection to an air sample bag.

FROG-5000TM User’s Manual

4.4.3. CONNECTING THE AIR SAMPLE ADAPTOR CONTINUED
1. Attach the air sampler to
the FROG sparge block. Be
sure the load/analyze
valve is pointed to RUN
AIR.
2. Connect the air sampler’s
power/control connector
to the air sampler power
port. This connection
supplies power to the air
sampler.
3. Attach
the
exhaust
connector to the FROG
exhaust with a twisting
motion.

If the Sparge Bottle Nut was completely
removed, make sure that the ferrule is
properly assembled before it is installed.
54

FROG-5000TM User’s Manual

4. A properly installed Air
Sampler will look like the
picture above.
5. The sampler can be used
with air sample bags using
the 1/16” to 3/16” adapter.
6. The sample bag adapter can
be replaced with the wand
for direct sample collection.
7. Connect the air sample wand
to the air sampler as
illustrated to the right.
8. It is advisable to install a
syringe filter on the end of
the
wand
in
dusty
environments.

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FROG-5000TM User’s Manual

Section 4: Field and Lab Analysis Procedures Continued

4.5: Loading a Sample
NOTE: The instrument should have a clean baseline to begin
sample loading and analysis. Refer to:
4.4: Instrument Preparation

To ensure optimal sample analysis, adhere to the following
sampling and cleaning procedures :


Liquid Sample:
a. Pull 5mL of the sample liquid into a syringe.
b. Transfer sample liquid to the sparge bottle.
c. Rinse sparge bottle and syringe between test runs.



Soil Sample:
a. Remove the sparge bottle from the instrument.
b. Add 1 g of soil to the sparge bottle with then add
5 mL of clean water. (See our website for an
application note on methanol extraction.)
c. Rinse the sparge bottle thoroughly between samples.
 Air Sample: Connect the air sampler adaptor and
wand to the FROG (Section 4.4.3).
When running an analysis (Section
4.6), hold the wand in the area
desired for sample collection.
If heavy concentrations are
detected, run a sample of clean
air to clear the system.

Detailed instruction begin on the following page.
57

FROG-5000TM User’s Manual

4.5. Loading a Sample Continued
•

If concentrations are known, during calibration for
example, a series of samples of the same analyte may be
run consecutively without rinsing the instrument. They
must be run in order from low to high concentrations to
prevent cross contamination.

LOAD
Position

Procedure:
1. Place load/analyze valve in the
WATER LOAD position (open).
(See Section 2.2.1)

2. Load sample into sparge bottle
 Liquid

Samples: Place sparge

bottle in DOWN position. Load 5

mL of sample through Sample
Inlet
 Silty Liquid Samples: Load 5 mL of

sample directly into the top of the
sparge bottle. Place sparge bottle
in DOWN position.
 Soil: Load 1 g sample directly into

the top of the sparge bottle. Fill
5mL syringe with deionized water.
Transfer the 5mL of deionized
water into the sparge bottle while
rinsing soil to the bottom. Place
sparge bottle in DOWN position.
58

DOWN

FROG-5000TM User’s Manual

3. Immediately place the load/analyze-valve in the RUN
WATER position (closed). The syringe may now be
removed.

ANALYZE Water
Position

4. Return sparge bottle to
the UP position. Rotate
the sparge bottle nut
counter clockwise until
finger tight to secure the
sparge bottle. The sparge
bottle should not spin in
the sparge bottle nut.

UP
NOTE: When the sparge bottle
is fully UP, the green sparge
tube is near the bottom of the
sparge

bottle.

partially

lowered sparge bottle will
cause analyte peaks to shift
right.

Sparge bottle
in the UP position.

FROG-5000TM User’s Manual

4.5. Loading a Sample Continued
The instrument display screen shows READY throughout
Steps 1-4 of Loading a Sample. The Log # is automatically
generated and assigned to the data file produced by the current
analysis cycle.

LOG#

FROG-5000TM User’s Manual

Section 4: Field and Lab Analysis Procedures Continued

4.6: Running A Sample
This section describes the procedure for running an analysis and
the instrument’s stages during an analysis cycle.
Procedure:
Record notes for future reference:
• If not connected to Ellvin™, the user should record the LOG
#’s and other relevant information for future reference. The
instrument records this number in the file name on the
internal SD card.
• If connected to Ellvin™, the user can record relevant
information for sample identification in the Ellvin™ Notes
section. Ellvin™ automatically stores the analysis data file in a
folder labeled with the date. The file itself is labeled with the
time it was created.

After the sample loading is complete (Section 4.5), the user may
begin sample analysis by pressing the start button. If connected to
Ellvin™, click the start button in the software. If not connected to
Ellvin™, press the black toggle button on the instrument.

Start Button PRESS
AT TOP
Start Button in EllvinTM

Continued next page.
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FROG-5000TM User’s Manual

4.6. Running a Sample Continued
During an analysis cycle, the FROG screen displays the analysis
steps in sequence as illustrated below and on the following
pages. This information is only visible on the instrument screen
(not the Ellvin™ screen).
Each sequence has a clock counting down the duration of the
current analysis step. The duration of the analysis step is

determined by the instrument settings.
(See Section 4.3)
The GC TEMP may vary slightly from the set point.

Analysis Sequence
1. Cleaning Prior to Sparging:
The PC is heated to drive off
contaminants (analytes from
previous runs).
2. Settle:
The PC cools before sparge
begins.

3. Collect:
VOCs are sparged and
collected on the PC.

Continued next page.
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FROG-5000TM User’s Manual

4. Release and Analyze, Settle:
The sample flow is switched to
the analysis channel.
The
pressure is allowed to stabilize
before the PC fires.

5. PC Fire:
The PC is heated and analytes
are injected (released) into
the GC column and continue
through to the PID.
6. Analyzing:
The PID measures analytes
as they emerge sequentially
from the GC.

7. Results:
If the analysis was started by
pressing the black toggle
button on the FROG, the
results will be provided on
the screen.

Continued next page.
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FROG-5000TM User’s Manual

4.6. Running a Sample Continued

Analysis Sequence Continued
8. Instrument Estimation:
When the instrument is not
connected to Ellvin™, it
makes an estimate of the
analytes’
concentrations
based on calibration data.

Press in top of Black Toggle
Button to see analyte results

Click at the top of the black
toggle button to scroll
through results. Press bottom
of toggle to go backwards.
The two estimates shown in
this example are 15 ppb
Toluene and 53 ppb o-xylene.
The GC retention time for
Toluene is displayed as 137 s.
NOTE: The instrument estimates are only available immediately
after the corresponding analysis. They are NOT stored on the
instrument’s SD card. Complete analysis chromatogram data are
stored on the SD card and can be imported to Ellvin for further
analysis.
9. Log File/WAIT:
The screen returns to the
original display and shows
the Log File # for the NEXT
analysis. WAIT indicates that
the GC temperature is above
the lower GC set point.
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FROG-5000TM User’s Manual

The FROG is ready for another sample loading and analysis once
the GC Temperature indicator appears green and READY is
displayed on the FROG screen.

Samples of the same analyte may be run consecutively from low
concentration to high without rinsing the instrument and
creating a clean baseline.

NOTE: If the user has run a high-concentration sample, it is
advisable to complete the following procedures:
 Liquid or Air Sample, perform:

4.4: Instrument Preparation

 Soil Sample: Remove sparge bottle from instrument and fill it

with deionized water. Rinse it thoroughly to remove all dirt
from bottle. Then, perform:

4.4: Instrument Preparation

Continued next page.

FROG-5000TM User’s Manual

Final Step: If desired, use Ellvin™ for detailed results analysis
after completing the analysis run.
• If the sample was run while connected to Ellvin™, open the

data file in Analyze Window and use Integrator 2.
(See Sections 5.2.1 and 5.2.3)
• If the sample was not run while connected to Ellvin, import
the data to Ellvin™ (Section 4.7), then open the file in
Analyze Window and use Integrator 2.

FROG-5000TM User’s Manual

Section 4: Field and Lab Analysis Procedures Continued

4.7. Importing Data to Ellvin
Ellvin™ allows the user to view live results, change operating
parameters, re-plot stored data and overlay data for comparison.
It also has tools for integrating peak areas, measuring retention
times , and calibrating the instrument.
The FROG-5000TM stores data from every analysis on a micro-SD
card. The following steps describe the process for extracting data
from FROG-5000TM for analysis with Ellvin™.
1. Locate the micro-SD slot in the head of the FROG.
2. Press in on the card and release to eject the card.
3. Use a micro-SD adapter to plug the card into a USB or SD
slot on a computer.

4. The micro-SD card performs as a thumb drive, and any file
management program (such as Windows Explorer) can be
used to move files.
Micro-SD Card Slot

FROG-5000TM User’s Manual

4.7. Importing Data Continued
3. The computer screen will display an option to “Open folder
and view files”. Open the folder.
4. The computer screen now displays a list of sequential files
named “LOG_XXX”. The file names are created and assigned
by the instrument. They appear on the instrument screen at
the beginning of each analysis cycle as shown in
Figure 4.7-1.

Figure 4.7-1: FROG-5000 display
screen at the beginning of a sample run.

NOTE: If the instrument is NOT connected to Ellvin™ when
running analyses, the user must record the LOG file numbers of
sample runs intended for future analysis. The LOG file numbers,
as well as the instrument estimates, are only available on the
instrument’s screen at the time the sample is run.
5. Select the LOG file of interest and drag/copy it to the
computer desktop or other location of user’s choice.

6. Open Ellvin™.
7. Select Live Data Tab.

8. Click on the OPEN button.

FROG-5000TM User’s Manual

9. Browse to the location where the LOG file of interest was
dragged/copied.
10. At the bottom of the “Select Data File” window, change
“Files of Type” to LOG Files.

11. Select the LOG file of interest and open it.
12. The computer screen now displays a chromatogram of the
file data as it is imported into Ellvin™. At this time, Ellvin™
also converts the data to an XML format and stores it. The
location path of the stored data is displayed at the top of
the chromatogram in the Live Data Window (Figure 4.7-2).
The data can now be opened in the Analyze Window.

Data Location Path

Figure 4.7-2: Live Data Window with
chromatogram and data location path.

FROG-5000TM User’s Manual

4.7. Exporting Data Continued
12. Click on the OPEN icon.
13. Follow the location path displayed in the Live Data Window to
the folder that contains the data file of indicated.
14. Location Path Example:

C:\Users\.....\......\EllvinData\20170426\1734_LOG_xxx.xml

16. The highlighted numbers correspond to the date the storage
folder and data file were created by Ellvin™. In this example,
April 26, 2017.
The folder contains a list of files. The file names are derived
from the time Ellvin™ converted the data. In the example in
Figure 4.7-3, the file named “1734” was converted at 5:34 PM
on 4/26/2017.

Folder name is the Date

File name is the Time and Log#

Figure 4.7-3: Data files named and stored by Ellvin™.

FROG-5000TM User’s Manual

Figure 4.7-4: File Selection Screen

17. A preview chromatogram of the selected file appears to the
right of the file list (Figure 4.7-4).
18. Click “Select” to open the file of interest. Ellvin™ will
automatically transfer to the Analyze tab and display the
data. Proceed to analyze the data with Ellvin™ Integrator 1
or 2. (See Section 5.2.3)

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FROG-5000TM User’s Manual

SECTION 5: ELLVIN TM SOFTWARE
The chromatogram software, EllvinTM, is primarily used to
perform detailed analyses of sample data. Ellvin™ uses
calibration data to:
• Assign analytes to chromatogram peaks based on GC
retention times.
• Report the analytes’ concentrations based on peak heights
and integrated peak areas.
Ellvin™ also manages the instrument settings and loads
calibrations to the instrument.
Several general features of Ellvin™, as well as a few features
specific to Analyze Window, are described in the following pages.
The user should be familiar with all the features to use the system
proficiently and look knowledgeable.

Software Features

Figure 5-1: Ellvin™ default screen details.

Tabs
To start using Ellvin™, refer to:
4.2: Connecting to Computer/Ellvin™
Continued next page.
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FROG-5000TM User’s Manual

Section 5. Ellvin™ Software Continued

5.1. FEATURES OF ELLVIN™
WINDOWS
The Ellvin Software has four windows that are accessed with
tabs when the software is open.

Window Tabs in Ellvin™
• Live Data Window: This is the default window for Ellvin™. In

Live Data Window, Ellvin™ creates a chromatogram from
sample analysis data. The data source may be:
a. From a sample run on the FROG while connected to
Ellvin™ and simultaneously imported to Ellvin™.
b. From sample data previously recorded on the
instruments SD card, then imported to Ellvin™.
• Analyze Window: As in Live Data Window, Ellvin™ creates a
chromatogram from sample analysis data. In this window,
Ellvin™ retrieves analysis data that was previously stored on
a computer. In Analyze Window the user can examine
chromatograms closely, integrate chromatogram peaks, and
export data to Excel.
• Calibration Window: In this window, Ellvin™ uses analysis
data from chemical standards to calibrate the FROG.
• Settings Window: In this window, Ellvin™ manages the
settings for the FROG. The settings define temperature and
duration parameters for various phases of the instrument’s
analysis cycle. Refer to:
4.3: Instrument Settings

FROG-5000TM User’s Manual

PORT
Use this feature to select the correct data port connection from
the FROG-5000TM. The user selects a COM# to connect to the
FROG.

Connection Port
Based on the selection of COM, the Connection Port will notify
the user if the FROG is CONNECTED or DISCONNECTED, and if the
parameters have been updated. (Bottom left corner of the
software display screen).

GC Column Temperature Indicator
This feature monitors and displays the GC temperature.
The icon is GREEN when instrument is ready for Loading.
It turns GRAY during sample analysis, and RED when the GC is
cooling.

FROG-5000TM User’s Manual

5.1. Features of Ellvin™ Continued

Start/Stop Button
Clicking on this button causes the FROG-5000TM to start an
analysis cycle. Once the analysis has begun, the red stop sign
appears. Clicking the stop button will stop the analysis and save
the chromatogram to the point that the run was halted.

OPEN Button
Click on this button to open saved files. The files can be from
data stored on a computer or on the instrument’s internal microSD card.

EXPORT
Export only works when Ellvin™ is in Analyze Window. The user
can export an opened file into Microsoft Excel program for further
analysis.

FROG-5000TM User’s Manual

Chromatogram Display Screen
The software display screen shows sample analysis data.
• The X-axis is time in seconds.
• The Y-axis shows the instrument’s PID response and GC

temperature (oC).
• The peaks correspond to separated analytes.

Sample Plot #2
GC Temperature

Sample Plot #1

Time (seconds)

Plots of other run variables can be toggled on and off by checking boxes in the
panel on the right portion of the screen.
Information such as the analysis pump
pressure and internal case temperature
are stored for every run.

GC Temperature (C)

Instrument PID Response

PC Fire

Analyte window
(based on calibration)

FROG-5000TM User’s Manual

5.1. Features of Ellvin™ Continued
Notes
This tool allows the user to records notes about samples while
in Live Data or Analyze Window. The notes are saved when the
sample analysis is completed. It is important to record the date
and time that data files are created in order to locate and
retrieve them for further analysis.

Magnifying Glass
Use this tool to zoom into a chromatogram while data is being
collected in Live Data Window, or while in Analyze Window to
help with integration.
Detailed instructions for using Magnifying Glass follow in
Section 5.2.2.

MOVE
Use this tool to move the chromatogram up and down the
Y-axis.

FROG-5000TM User’s Manual

Integrator 1
This feature only operates while in
Analyze Window. The user creates a
baseline and integrates peaks, then
calculates the peak height and area. The
baseline integration limits are set by
clicking the left mouse button.
Integrator 2
This feature only operates while in
Analyze Window. Ellvin™

creates a

baseline by snapping vertically from the
left mouse click position to the data line.
Ellvin™ then calculates the peak height
and area. Integrator 2 is recommended
for the most consistent analysis results.
Detailed instructions for using Integrator
2 follow in Section 5.2.3.
Data Grid
Use with Integrator 1 and 2. The Data Grid shows:
•

Analyte (Toluene) Based on calibration retention times.

•

Peak Reference Number (1)

•

Retention Time (138.08s)

•

Peak Height (732529)

•

Peak Area (7960892)

•

Calculated concentration (14.1037) Based on calibration
concentrations.

FROG-5000TM User’s Manual

Section 5: Ellvin™ Software Continued

5.2. APPLYING ELLVIN™ FEATURES
5.2.1. OPEN BUTTON
To open stored files in Analyze Window:
•

Click on the Analyze Tab.

•

Click the OPEN button.

•

A file selection screen appears.
(Figures 5.2.1-1 and 5.2.1-2)

NOTE: Ellvin™ automatically labels folders and files as they are
created.
 Folders of data files are labeled by the DATE they were

created.
 Data files are labeled by the TIME they were created and

the LOG number.
In the example shown in Figure 5.2.1-1, the data file was created at 10:01 AM on April 26, 2017.
•

To locate the data file of interest, browse to the folder
labeled with the date the sample was ran, or the date the
data file was imported from the FROG to Ellvin™. Double
click to open the folder.

•

Click on a data file of interest. A preview of the data
chromatogram appears to the right of the list.

•

Click “Select” to open the file for analysis with Integrator 2.
80

FROG-5000TM User’s Manual

Folder name is the Date

File name is the Time

Figure 5.2.1-1 Data files

Figure 5.2.1-2: File Selection Screen

FROG-5000TM User’s Manual

5.2. Applying Ellvin Features Continued

5.2.2. MAGNIFYING GLASS
The Magnifying Glass can be used in Live Data Window during
data import, or while in Analyze Window.
Click on the magnifying glass icon to start.

To Magnify:
• Left click and hold
• Drag
mouse
over
desired
magnified
area.
(Figure 5.2.2-1)
• Release the mouse button.
• The area being magnified is highlighted light blue.
• The display screen then readjusts to show the magnified area.
(Figure 5.2.2-2).
To De-Magnify:
• Click the circle on the scroll bars.
• OR double click the magnifying glass icon.

FROG-5000TM User’s Manual

Figure 5.2.2-1: Desired Magnified Area

Figure 5.2.2-2: Magnified Area

FROG-5000TM User’s Manual

5.2. Applying Ellvin™ Features Continued

5.2.3. INTEGRATOR 2
Integrator 2 can be used only in Analyze Window. This tool calculates the area under a peak and the peak height. The area or
peak height helps determine the concentration of the analyte.
Click on the Integrator 2 button to start.

Technique to Integrate:
(Refer to Diagrams 5.2.3-1 and 5.2.3-2)


Begin at the lowest point, Valley 1.



Drag the mouse to draw an integration line, past the peak,
to the second lowest point, Valley 2.



The integration line should NOT cross the data line. Avoiding this ensures the correct calculation for the peak area.
(Figure 5.2.3-2).

FROG-5000TM User’s Manual

Peak

Integration Line
Valley 1

Valley 2

Diagram 5.2.3-1: Valleys and Peaks

Peak

Valley 1

Integration
Line

Valley 2

Diagram 5.2.3-2: Crossing Data Line

FROG-5000TM User’s Manual

5.2.3. Integrator 2 Continued

Integrating a Single Peak
Using the correct technique illustrated on the previous page,
select the desired peak for integration.


Select Integrator 2.

2. Left click the mouse and hold at Valley 1.
3. Drag to Valley 2 and release.
4. The Integrator 2 tool uses the software to select the baseline on which the integration line is drawn.
5. The gray shadowed area in Ellvin™ will show which area is
being integrated. (Figure 5.2.3-3)
6. The integration is complete when a solid black line appears.
7. The Data Grid shows the data that corresponds to the related peak number near the base of the peak. The user can
continue integrating peaks.

FROG-5000TM User’s Manual

Figure 5.2.3-3: Desired Peak Integrated Area

Figure 5.2.3-4: Integration Line

Data Grid

FROG-5000TM User’s Manual

5.2.3. Integrator 2 Continued

Integrating Multiple Peaks
To integrate multiple peaks, follow the same procedures for
integrating a single peak using Integrator 2.
Click on the Integrator 2 button to start.


Right click and hold the mouse at Valley 1.



Drag to Valley 3 and release.



The integrator uses Ellvin to select the baseline on which the
integration line is drawn.



The integration is complete when a solid black line appears.
The result shows the integration line and the splits placed
between joined peaks. (Figure 5.2.3-5)



To integrate the remaining peak (in Figure 5.2.3-5, Peak 3).
Click and hold at Valley 3 and drag to Valley 4.



The Data Grid shows the data that corresponds to the related
peak. The user can continue integrating peaks.

Valley 2

Valley 3
Valley 4

Valley 1

1
Figure 5.2.3-5: Integration of multiple peaks complete.

Data Grid

FROG-5000TM User’s Manual

When integrating multiple peaks it is necessary to follow the baseline as closely as possible. It is recommended that each peak be
carefully assessed before integration. It is usually best to integrate
each peak separately unless the valley between the peaks does
not reach the baseline as in the case of peaks 3 and 4 in the figures
below (Figure 5.2.3-6 and Figure 5.2.3-7).

Figure 5.2.3-6: Improper Integration of Multiple Peaks

3 4

Figure 5.2.3-6: Improper integration of Multiple Peaks

Figure 5.2.3-7: Proper integration of Multiple Peaks

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FROG-5000TM User’s Manual

SECTION 6: CALIBRATION
This section provides detailed instructions for calibrating the
instrument.
The system is calibrated by testing a series of analytes at varying,
known concentrations. As with all GC work, these analytes are
selected from an established list of chemicals that are of
foremost interest in the environment under investigation.

• Before beginning a calibration, it is recommended that the user

become familiar with EllvinTM. Refer to Section 5.
• The user should also be familiar with analytical techniques for

GC calibration.
• The preparation and use of standards for calibrating the

instrument differs for air, liquid, and soil samples. Section 6.1
introduces the procedure for preparing standards.
• Section 6.2 provides detailed instruction for using the features

found in Calibration Window.
• Section 6.3 provides detailed instructions for calibrating the

instrument.

FROG-5000TM User’s Manual

Section 6: Calibration Continued

6.1. CALIBRATION STANDARDS
NOTE: The instruction in Sections 6.1.1 and 6.1.2 correspond to
Step B-3 in Section 6.3.

6.1.1. STANDARDS FOR LIQUID OR SOIL SAMPLES
For screening purposes, a calibration can be created with a single
known sample. However, it is best to run at least four
calibration samples from low to high concentrations.

General concepts to calculate the dilutions
Traditionally, ppb concentrations of analytes in water are
expressed in micrograms of analyte per liter of water (=1 Kg
water). Calibration standards come pre-diluted in methanol.
Methanol cannot be ionized (and therefore not detected) by
the instrument’s PID.
The standards are frequently referred to in terms of ppm, but if
you carefully look at the packaging, somewhere, it should say
x.x-mg analyte/mL-Methanol. For example, a common fuel
mixture, BTEX, will be provided as 2.0-mg/mL-methanol and
often this is referred to as a 2000-ppm standard because it is
2000 g BTEX/mL methanol.

The important thing to follow is the mass of the analyte in the
sample. If you draw 5-L of the 2000 ppm BTEX solution into a
syringe, the syringe contain 0.005-mL x 2000-g/mL (or 10 g)
of benzene, 10 g of toluene, 10 g of ethylbenzene, and so on.
If you inject this 5-L syringe BTEX standard into 5-mL of water,
there will be 10-g of benzene in the 5-g of water, In other
words, the benzene contamination is 2-µg benzene/1-g water =
2000 ppb.
92

FROG-5000TM User’s Manual

Handy things to know:
1 µg/mL = 1 mg/L = 1 ppm (mass)

1 µg /L = 1 ppb (mass)

BTEX = Benzene, Toluene, Ethylbenzene, p/m-Xylene, o-Xylene

Diluting 5mL Water with Standard
The density of water is 1 g/mL, so if a syringe contains 5 mL of
water, it means that there are 5 g of water in the syringe.
If you have a 200ppm benzene standard, what is the resulting
concentration from injecting 2µL (=0.002 mL) of the 200ppm
benzene standard into the 5-mL water filled syringe?

There will be a concentration of 80 ppb benzene in the water.

Diluting from a Standard
Let CA = original concentration of an analyte in a solvent, and F =
desired dilution factor so CB = CA / F. Find VA = volume of mix CA
that must be added to a solvent volume VB to achieve
concentration CB, where mA is the mass of analyte in VA.

(approx. for low CA)

Solving for VA yields:
Example: Using CA = 2000 µg/mL BTEX in MeOH standard to make
a CB = 200 µg/mL BTEX in MeOH standard (that is F = 10), you must
add 0.1 mL of CA to 0.9 mL MeOH to make a 1 mL standard of 200
µg/mL BTEX in MeOH.
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6.1.1. Standards for Liquid and Soil Samples Continued
Preparation Continued:
2. Fill a syringe with 5 mL deionized water.
3. Load a known volume, as calculated on the previous pages,
of a liquid standard into the syringe.
4. Connect to Ellvin™ and follow the sample loading instructions
in Section 4.5.
4.5: Loading a Sample

6.1.2. STANDARDS FOR AIR SAMPLES
Gas calibration standards are available from several sources,
and we recommend searching the internet with the term
“Calibration Gas Standards.” The standard typically comes in a
pressurized cylinder with a mixture of analytes in nitrogen to
achieve the proper dilution. A typical mix for calibration would
be 100 ppbv BTEX in N2. Concentrations are typically in parts
per billion by volume.
A regulator on the bottle controls the flow and pressure at the
exhaust. Because sample will be collected directly from the
atmosphere, we suggest connecting the FROG’s sample tube to
the bottle with a Tee. One port on the tube should vent to the
atmosphere and flow of the sample gas should exceed the
collection flowrate of the FROG (~60 mL/min).
The FROG permits the creation of a single point calibration, and
this is adequate for many applications. Results will be good
near the calibration gas concentration, but less reliable further
from that concentration. Multiple point calibrations are
preferred.
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6.1.2. STANDARDS FOR AIR SAMPLES (CONTINUED)
Run at least four calibration standards from low to high
concentrations. Defiant Technologies sells a portable gas diluter
accessory to be used with the FROG for air calibrations.
Tools Needed:
• Portable Gas Diluter
• Calibration Gas
• Air Sampler

Gas Diluter attached to Calibration gas and FROG

Instrument Preparation:
• Connect Air Sampler to instrument and then connect air
sampler to the gas diluter. Place load/analyze-valve in the
load position (open). (See Section 4.4.3 Connecting the Air
Sample Adapter).
• Connect the calibration gas to the gas diluter.

Continued next page.
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6.1.2. Standards for Air Samples Continued.
Standard Preparation and Loading:
1. Use the spreadsheet provided with the Gas Diluter. This will
allow for the calculation of sample concentration using the
starting concentration of the calibration gas.
2. Adjust the flow rates on the gas diluter until stable at the
desired rates.
3. Press the start button and run sample.
Refer to:
4.6: Running A Sample
4. Repeat for other sample concentrations.

For more detail on the air calibration procedure using the Gas
Diluter please refer to the Gas Diluter User’s Manual.

Another method to perform multiple point calibrations is to buy
multiple compressed standards, or alternatively, to prepare
multiple concentrations in Tedlar™ gas sample bags. We will not
go into details on these procedures. We will point out, however,
that it can be difficult to achieve accurate concentrations much
below 20 ppbv. Low concentration samples tend to degrade in
compressed cylinders, and sample bags often have contaminants
that appear as large peaks in the chromatography. It is still
possible to achieve reasonable results, and a Frog user is welcome
to call Defiant Technologies for further discussions. Contact
information can be found at www.defiant-tech.com.

FROG-5000TM User’s Manual

Section 6: Calibration Continued

6.2. ELLVIN™ CALIBRATION WINDOW FEATURES
• To start using Calibration Window, open Ellvin™ and click on the

Calibration Tab.

6.2.1. LIST OF ANALYTES
This feature allows the user to create a List of Analytes that may
be used to calibrate the instrument. The analyte names in the List
of Analytes will later be associated with analyte peaks from
calibration standard runs. (See Figure 6.2.1)
The Retention Time, Peak Height, and Peak Area in Figure 6.2.1 are
generated from previous analyses of standard solutions with
known concentrations.

Figure 6.2.1: Typical entries used to build a calibration file.

FROG-5000TM User’s Manual

Creating a new calibration








Click on the New File Icon
Click on the New Analyte
Icon
At this point, a pop-up
window appears
requesting an analyte
name and an alias that will
appear on the FROG
display after a run. In this
example, the alias BENZ is
given for benzene.
Click OK and this analyte
will be added to a list of
analytes.
Add as many analytes as
needed for the calibration.
If you want to delete one,
just click on the name, and
use the delete button on
the computer.

At this point, you are ready to run calibration standards to produce the calibration data. That data will be peak heights and integrated peak areas for multiple concentrations for each analyte.
Because the samples are separated in the chromatogram, multiple analytes can be tested in a single sample run.
For each analyte, the peak area (or height) will be correlated with
the concentration through a linear or quadratic equation.

FROG-5000TM User’s Manual

6.2. Calibration Window Features Continued

6.2.2. CORRELATION CHART AND EQUATIONS
This feature in the Calibration Window displays the degree of
correlation between analyte standards of varying concentrations
and the instrument’s response to them.
(See Figure 6.2.2)
The correlation is calculated as both a linear equation and a 2nd
order quadratic equation.
The user may choose to view the equations and a chart for
either Peak Area correlation or for Peak Height correlation.
This correlation is important because Ellvin uses these
parameters to calculate concentrations for future samples.

Figure 6.2.2: Correlation Equations and Chart

FROG-5000TM User’s Manual

Section 6: Calibration Continued

6.3. CALIBRATION PROCEDURE
The Calibration Procedure consists of running a series of analysis
cycles with standards of varying concentrations for a given
analyte. EllvinTM then uses the analysis data for the standard
solutions to calibrate the instrument.

Before beginning a calibration, the user should be familiar with:

4.3: Instrument Settings

4.4: Instrument Preparation

4.5: Loading a Sample

4.6: Running A Sample

5.1-5.2: ELLVIN™ Software

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It is recommended to run a minimum of four standards, from low
to high concentration, to create data points for calibrating the
instrument.
The concentration range for calibration standards depends on the
analyte and its unique retention time.
Example standard concentrations for a calibration: 2 ppb, 10 ppb,
50 ppb, 100 ppb

Calibration Procedure Steps
A. Add the Calibration Analytes to the List of Analytes.
(See Section 6.2.1)
The steps in this section prepare the software to calibrate using
selected analytes.
1.

Open the Calibration Window.

2. Begin by adding each of the analytes chosen for calibration, one
at a time, to the Analyte List.
(See Section 6.2.1)
•

No data will be displayed and the chart display area will be
blank. Disregard any equations displayed.

Continued next page.
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6.3. Calibration Procedure Continued

B. Run the Calibration Standards
In this section, the calibration samples are loaded and instrument
response data is stored.
1. Open the Live Data Window

Create a clean baseline before running any calibration
samples. Refer to:
4.4: Instrument Preparation

To begin the calibration, load and run a sample containing
only deionized water. Refer to:
4.5: Loading a Sample
4.6: Running A Sample

Load and run a minimum of four calibration standards of the
selected analytes. The samples should be of varying
concentrations and should be run in order from low to high
concentrations. See Section 6.1 for sample preparation.

For each of the calibration standards, record the
concentrations in the Notes window. This will help identify
the files during analysis and calibration. All of the system
configuration parameters are automatically stored.

Write sample mix and concentration and other notes here

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After the instrument has completed running the four (or more)
calibration standards, go to the Analyze Window in Ellvin™ to
analyze the instrument response data.
C. Analyze the Data
In this window Ellvin™ analyzes the stored instrument response
data to create the information needed for calibration.

1. Open the Analyze Window

2. Click on the OPEN button to access a list of previously run
sample files. (Figure 6.3-1)
(See Section 5.2.1 for details)
3.

Select the calibration sample file desired for data analysis.
The files are labeled by the time they were created and the
LOG number.

Figure 6.3-1: Open Sample Files

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6.3. Calibration Procedure Continued
4. In the Analyze Window, click on the Integrator 2 button to
integrate the sample peaks in the chromatogram.
(Refer to Section 5.2.3 for detailed instructions on
using the Integrator 2)
The results of the data analysis are displayed on the
chromatogram and in the Data Grid. (See Figure 6.3-2).

The example shown in Figure 6.3-2 is for a sample of MBTEX.
Each peak corresponds to an analyte in the MBTEX mix. Analytes
may be run one at a time to determine or confirm retention
times. (In this figure, Peak #1 is MTBE which was included in the
mix). The third peak at 206 seconds is toluene. This could be
confirmed by running toluene by itself.

Double left click mouse here and your list of analytes will appear.

Figure 6.3-2: Analysis Chromatogram and Data Grid

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D. Calibrate the Instrument
In this section, the data analyzed in Section C is transferred to the
Calibration Window. Ellvin™ uses the data to calibrate the
instrument for each analyte.
1. To assign peak data to an analyte name, double left click the
drop-down arrow under Analyte on the Data Grid. A List of
Analytes previously recorded in the Section A of the
Calibration Procedures appears.
2. Left click on the analyte to assign that name to the
corresponding peak.
3. Left click to highlight a row then
right click, and a pop-up appears.
Left clicking the Identified Analyte
will copy the peak information to the
calibration table. A pop-up window will appear asking if the
selected data is to be copied to the calibration
file. Click ‘yes’. Continue to match all the
peaks with an analyte to the calibration file.
Holding the shift key down will allow multiple
analytes to be selected and copied to calibration
simultaneously.
Figure 6.3-4: Toluene is Selected for Peak #3.
Figure 6.3-3: Assigning Analytes to
Chromatogram Peaks
Toluene

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6.3. Calibration Procedure Continued
4.

To view the imported data, open the Calibration Window,
click on List of Analytes, and select the desired analyte. The
imported data for this analyte will be displayed

In the example in Figure 6.3-5, Toluene has been selected.

Figure 6.3-5: Select the Desired Analyte from the List of Analytes

In the Calibration Window, enter in the corresponding
concentration for the calibration standard in the column

titled “Concentration”.

In this case, the toluene

concentration was edited to be 40 because a 40 ppb
standard was used in creating the chromatogram.
Standard concentrations were recorded in the Notes
window in Section B of the Calibration Procedures.
(See B-4.)

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Repeat Steps C-1. through D-5. of the Calibration Procedures to
import and enter all the standard data to the Calibration
Window.
The Correlation Chart and Equations (Section 6.2.2) will appear
after the first point is imported into the Calibration Window. The
single linear point fit will be automatically forced through zero.

As data points are added, correlation equations are generated by
the Ellvin™. A graph of the data points and the curve fit will be
displayed in the calibration window. The user can now inspect the
fit equation.
Downloading the Calibration to the Instrument
Connect the instrument to Ellvin™ with the USB cable. Refer to:
4.2: Connecting to Computer/Ellvin™
1. All analytes listed in the calibration should show a R2
correlation close to 1.0. The closer the R2 value is to 1.0, then
the better the software can calculate the concentration of an
unknown sample.
2. Open the Calibration Window.
Continued next page.
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6.3. Calibration Procedure Continued
3. Click on ‘Download Calibration to Device’

4. A popup with several different options will appear. By default
the software will set to download the calibration file currently
loaded in the calibration tab. Select the appropriate option
and then click “set” to download the calibration to the FROG.

5. This will allow the instrument to estimate the concentration of
analytes uploaded into the calibration using the peak height
linear function.
NOTE: The Ellvin™ software can compute concentrations using
either peak height or area under the peak. However, when the
instrument is running independently, its processing power is more
limited, so peak height and a linear data fit are used in calculating
concentrations.
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SECTION 7: MAINTENANCE
7.1. ROUTINE CLEANING
It is important to clean the FROG-5000TM after analyzing samples
in order to prevent cross-sample contamination.
The FROG-5000TM does need to be cleaned if:
• More than one type of analyte is being tested. The user

must clean the instrument between sets of the different
analytes.
• A high concentration is introduced. If the user observes
large peaks in the chromatogram, the instrument should be
cleaned prior to analyzing a sample.
The FROG-5000TM does NOT need to be cleaned immediately if:
• Same analyte is being tested. The user can run a set of

samples of the same analyte from a LOW concentration to
a HIGH concentration, consecutively. The user cleans the
instrument at the end of the set.
• Rinsing the sparge bottle and syringe between uses is

always recommended.
• The baseline is clear. If no peaks are observed in the
chromatogram, cleaning is not necessary.
To clean the instrument, perform the following procedures:

4.4: Instrument Preparation

The instrument is clean when a clean baseline has been achieved.
This means that only small waves appear in the chromatogram
(see Figure 7.1-1 on next page)
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Section 7.1: Maintenance Continued

Figure 7.1-1: Chromatogram of a clean baseline. The small peaks around 40 and
60 seconds will not matter if chemicals of interest do not elute at these times.
Even if a chemical in the sample elutes at say 60 seconds, the peak would be
subtracted in the calibration if this small peak is a constant area between runs.

7.2 CLEANING THE SCRUBBER
It is recommended to clean the carrier gas scrubber every
three months, or as needed to create a clean baseline.

Supplies needed to clean the
scrubber include:

1. *Clean Activated Carbon
2. *Clean Molecular Sieves
3. 3/32” allen wrench
Figure 7.3-1: Supplies needed.

*NOTE: Replacement activated carbon and molecular sieves
may be purchased, premeasured and cleaned, through Defiant
Technologies, Inc.
Activated Carbon, Part # DT-FG4K-AC-1
Molecular Sieves, Part # DT-FG4K-MS-1
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FROG-5000TM User’s Manual

1. Remove the four corner 4-40 screws with the 3/32” allen
wrench.
2. Lightly pull the scrubber off the FROG; place any O-rings
back in place if they come out.
Figure 7.3-2: Carrier gas scrubber is
removed by taking out the 4 corner
screws with the 3/32” allen wrench.
Carrier Gas Scrubber
Corner screw

3. The Carrier Gas Scrubber only goes on in one orientation.
• The small screw in the side of the block faces the display

when the block is properly oriented.
• There are two chambers in the block. The back chamber
(closest to the display) contains molecular sieve. The front
chamber holds activated carbon.

Small screw must face
the display when
scrubber is installed.

Figure 7.3-3: Carrier Gas Scrubber removed from the Sparge
Block.

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7.2 Cleaning the Scrubber Continued
4. Hold the scrubber lid-side up and remove the two,
center position, 4-40 screws with the 3/32” allen wrench.

Figure 7.3-4: Two Center Screws hold the lid on the scrubber block.

Back Chamber

Front Chamber

Back Chamber

Front Chamber

Note: Glass wool
packing is in each of
these tubes.
The
wool prevents carbon
from entering the
sparge manifold. The
wool should not be
removed.

Figure 7.3-5 Open scrubber
5.

Remove the scrubber lid; put any O-rings back in place if
they come out.
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6. Dispose of dirty activated carbon and molecular sieves.
7. Use a paper funnel to put new activated carbon into the front
chamber.
8. Use a paper funnel to put new molecular sieves into back
chamber.
9. Replace the lid.
10. Replace the two, center position, 4-40 screws and snug them
tight with the 3/32” allen wrench.
11. Place the scrubber back onto FROG. Make sure small screw in
the side faces the display and the bottom tubes slide into the
proper holes in the sparge manifold.
12. Replace the four corner 4-40 screws and snuggly tighten with
the 3/32” allen wrench.
13. There should be NO gap between the sparge manifold and the
scrubber block! (Figures 7.3-6 and 7.3-7)
14. To fix space in between, remove the block and make sure
O-rings are in the proper position.

No Space

Space

Figure 7.3-6:
Correct scrubber position.

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Figure 7.3-7:
Incorrect scrubber position.

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SECTION 8: TROUBLE SHOOTING
8.1. INVERTING THE FROG WITH WATER IN THE
SPARGE BOTTLE
If the FROG has been inverted with water in the sparge bottle, it is
likely that water will enter the sparge manifold.

Scrubber

Sparge
Manifold

If the FROG was not running at the time, it is unlikely that the
water went very far through the manifold. Below is a transparent
view of the sparge block that may aid in performing steps on the
following page.

This path must be
cleared of water.

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8.1. Inverting the FROG Continued
1. Remove the sparge bottle and turn the load/analyze valve
handle to RUN AIR. Connect a clean, dry syringe to the
exhaust port from the gas module and push air through the
port. DO NOT use a compressed gas can.
Figure 8.1-2: Pushing water
from the plumbing.

Sparge Manifold
Sealing Screw #1

2. Remove sealing screw #1 and re-install an empty sparge
bottle. Push air again through the exhaust port with the
clean, dry syringe.
3.

Replace sealing screw #1 and remove sealing screw #2 on
the opposite side of the sparge manifold. Push air again
through the exhaust port with the syringe.

3. Replace sealing screw #2 and remove the scrubber block.
Look for water. Push air again through the exhaust port
with the syringe.
Figure 8.1-3: Pushing
water out of the
sparge manifold.

Sealing screw #2.
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If there was water under the carrier gas scrubber block, the
scrubber materials will need to be removed and replaced.
Re-install the carrier gas scrubber block and remove the
syringe from the exhaust port. Remember that the small
screw in the side of the scrubber block must face the
display. (See Section 8.1.3, Cleaning the Scrubber for
procedure details)

Press the start button on the FROG and allow the system
to run 20 seconds through the collect then turn the FROG
off . If you do not see water coming from the exhaust port,
everything is probably fine.

7. Load a blank sample (deionized water) and run a Collect
Cycle (make sure the Load/Analyze Valve is turned to RUN
WATER).
Look for bubbles in the sparge
bottle.
• If bubbles appear, the liquid

was successfully removed
from the sparge manifold.
• If bubbles do not appear,

then contact Defiant Technologies for consultation.

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Figure 8.1-4: Look for Bubbles
in the sparge bottle.

FROG-5000TM User’s Manual

8.1. Inverting the FROG Continued
8.

Perform some analyses with clean water and see if the
chromatograms look normal.

Refer to:

4.6: Running A Sample

9. Contact Defiant technologies or your local representative for
further assistance if the chromatograms continue to look
erratic. Let us know what happened, we cam fix it much
quicker that way. Contact information is at www.defianttech.com.

Email: info@defiant-tech.com
Phone: 505-999-5880

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Section 8: Troubleshooting Continued

8.2. DEGRADING CHROMATOGRAPHY :
SCRUBBER IS NOT SECURED PROPERLY
If the Chromatography is degrading over time:
Check the scrubber block.
The scrubber block may NOT be sealed correctly.
There should be no space in between the scrubber block and the
scrubber holder.
(Figures 8.2-1 and 8.2-2)
(Refer to Section 8.1.3, Cleaning the Scrubber)

No Space

Space

Figure 8.2-2:
Incorrect scrubber position.

Figure 8.2-1:
Correct scrubber position.

Performance can also be affected by high concentrations of
analytes, or high background concentrations in the
surrounding environment (See next section).

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Section 8: Troubleshooting Continued

8.3. CANNOT ACHIEVE A CLEAN BASELINE
If a clean baseline cannot be achieved after repeatedly following
the procedures in Sections 4.4.1 and 4.4.2 (Rinsing the
Instrument and Creating a Clean Baseline), contamination
problems may stem from having loaded a sample with a high
concentration of analyte, or from the presence of airborne
contaminants.
NOTE: A small peak at the beginning of the chromatogram is
normal for a clean baseline.
Complete the following procedures to eliminate contamination
interfering with a clean baseline.
Perform procedure 4.4 while connected to Ellvin™ and using
warm deionized water. Refer to:
4.4: Instrument Preparation
Repeat this procedure up to three times if necessary to achieve a
clean baseline.
Airborne Contaminants
If it is still NOT possible to achieve a clean baseline when
running water samples, there may be contaminants present in
the ambient air. An airborne contaminant can enter the sparge
bottle when it is lowered. This volume of air cannot be scrubbed
and will be collected by the PC.
Airborne contaminants can also enter through the pump with
the sparge and carrier gas though these are largely removed by
the scrubbers.
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Solutions:
1. Either circulate air in the testing environment, or move to a
new location, then perform:
4.4: Instrument Preparation

If a clean baseline is still not achievable,
2. Clean the scrubber.
(See Section 7.3)
If a clean baseline is still not achievable,
3. Provide an activated charcoal trap at the pump inlet (V).

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Section 8: Troubleshooting Continued

8.4. COOL FEATURES (CONTINUOUS OPERATION)
Continuous Sampling and Analysis Mode
If the FROG-5000TM is not connected to a computer, a continuous
run cycle can be initiated depressing the upper part of the black
toggle switch when the power switch is simultaneously turned
on. The next time the toggle is depressed, the unit will begin continuous cycling. No chromatography results will be displayed on
the screen. End the cycle by switching the power off. All chromatographs can be recovered from the internal micro-SD card.

2. SLIDE UP

1. PRESS IN

When the FROG is connected to EllvinTM, it can be set to cycle
continuously by simply holding the shift key down when the start
button is pressed. If this action is initiated, a pop-up window appears
where cycle numbers and time delays can be set. Remember to account
for the cooldown time in setting interval times.

If an interval is not
set, the cycle will
restart when the GC
cools to its low temperature setting

Continuous cycling is particularly useful in monitoring air or running water blanks for cleaning the instrument.
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Section 8: Troubleshooting Continued

8.5. REALLY USEFUL FEATURES
Frog Doctor
Frog-Doctor provides the ability to turn valves and pumps on and
off without performing sample runs. The Frog Doctor button appears in both the live data and the Settings windows.

The FROG must be communicating through ELLVIN for Frog Doctor to work. If the FROG is connected and the Frog Doctor button
is clicked, a pop-up menu is displayed with several options as displayed in Figure 8.5.1.
Energize bypass
valve

Energize sparge
valve

Start
pump
Fire PC
Turn PID
off

Change sparge pump
speed (range 0-100)
Change analysis
pump speed

Get frog doctor
window.

Implement
pump change
Implement
time changes

Alter date and
time on microSD data

Implement
log# changes

Toggle display
backlight

Reading of absolute
pump pressure

Figure 8.5.1. Features of Frog Doctor
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SECTION 9: CARE OF THE LI-PO BATTERY
The FROG-5000 uses 10.8 volt lithium polymer batteries that are manufactured by Inspired Energy. Check their website for additional information on the battery, www.inspired-energy.com. In particular, check
the website at www.inspired-energy.com/shipping.html for up to date
information on shipping the battery. The exact part number for the
battery is NF2040HD34.
9.1.1. Handling
• Avoid shorting the battery and do not immerse in water.
• Do not disassemble or deform the battery
• Do not expose to, or dispose of the battery in fire.
• Avoid excessive physical shock or vibration.
• Keep out of the reach of children.
• Never use a battery that appears to have suffered abuse
9.1.2. Storage: Store in a cool, dry and well-ventilated area.
9.1.3. Disposal: Dispose of in accordance with local regulations.
9.2. General Specifications: Nominal Voltage: 10.8V, Rated Capacity:
Initial capacity is 6460mAh

9.3.1 Shipping: check www.inspired-energy.com/shipping.html for up to
date shipping information. As of this time this manual was written, the
guidance is:
•

Battery may be shipped in the equipment by ground or cargo aircraft
with no special packaging labels

•

Battery cannot be shipped on passenger aircraft as checked baggage.
It must be removed from the Frog , packed so it cannot crush or
short, and hand carried by the passenger on the aircraft.

•

If the battery is shipped outside of a unit it should
have less than a 30% charge before transport. Run unit
until only one bar shows on charge indicator, then
remove the battery and pack for shipping.

•

These guidelines may not apply for all countries and
the user should check for local requirements for shipping lithium polymer batteries.
Remove both thumbscrews
then slide heel straight back
to remove

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SECTION 10: POSITIONING OF THE SPARGE NUT
There is a stop on the sparge bottle nut cover that prevents the nut from
being completely removed or overtightened. This prevents the accidental loss of the Teflon™ ferrule parts that seal the sparge bottle to the
system. The ferrule parts will compress over time and it will be necessary
to reposition the nut stop to allow the ferrule to seal better against the
sparge bottle or air sample adapter. To reposition the nut stop, simply
slide the plastic sparge nut cover off of the hex nut and rotate it one or
two positions counter-clockwise. Press the cover back on the hex nut
and check that the nut will tighten better on the sparge bottle. The
Teflon™ ferrule is compliant so it is not necessary to tighten hard against
the glass sparge bottle. The nut should provide a snug fit to prevent gas
from leaking from the system when the unit is running. If the chromatography peaks appear to have shifted to the right (later in time), it is
possible that gas is leaking from the system at the sparge bottle nut.

Never try to rotate
this hex cap
Hex Nut
Nut Stop Tab
Rotate this nut cover CCW to
the next hex position for a
tighter grip on the sparge
bottle
Pull cover off of the hex nut
for repositioning
Repositioning the sparge nut stop.

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126

FROG-5000TM User’s Manual

APPENDIX I: AVAILABLE ACCESSORIES/SPARE PARTS
Item
FROG-5000
Warranty and
Service Contract

Defiant Part #

Description

DT-FG-SERV-1

One year service
contract / maintenance
program.

DT-FG-AC-1

Replacement Activated
Carbon for FROG air
scrubber.
Pre-measured and
pre-cleaned.

DT-FG-MS-1

Replacement Molecular
Sieves for FROG air
scrubber. Pre-measured
and pre-cleaned.

DT-FG-SBGN-1

Sparge Bottle with globe
neck.

DT-FG-SBN-1

Nut to connect Sparge
Bottle to instrument.

DT-SYGG-1

5mL glass syringe with
Luer Lock tip.

Activated Carbon

Molecular Sieves

Sparge Bottle

Sparge Bottle Nut

5 mL Glass Syringe

127

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APPENDIX I: AVAILABLE ACCESSORIES/SPARE PARTS
Item

Defiant Part #

Description

DT-FG-SYGP-1

5mL plastic syringe
with Luer Lock tip.

DT-FG-SYGN-1

60mL syringe with Luer
Lock tip for rinsing
Sparge Needle.

DT-FG-LL-1

Attachment for
syringe.

DT-FG-PID-1
DT-FG-PID-2
DT-FG-PID-3

PID Assembly for
normal sensitivity (1)
low sensitivity (2)
high sensitivity (3)

DT-FG-TK-1

Assorted tools for
maintenance of
FROG

DT-NEEDCOV-1

Sparge Tube Guard for
covering Sparge tube
in shipping container

5mL Plastic Syringe

60mL Rinsing Syringe

Luer Lock Inlet

PID Assembly

Tool Kit

Sparge Needle Cover

128

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APPENDIX I: AVAILABLE ACCESSORIES/SPARE PARTS
Item

Defiant Part #

Description

DT-FG-SBNF-1

Replacement
ferrules for the
Sparge Bottle Nut,
1/2" ferrule set.

DT-FG-SBNF-2

Replacement
ferrules for the Air
Sample bag adapter
for the FROG’s air
sampler, 3/16"
ferrule set, Teflon.

DT-FGSL-1

Sparge Line with
Connectors.

DT-FG-VLV-1

Replacement valve
assembly for FROG5000.

DT-FG-AS-WAND

Replacement air
sampler wand.

DT-AS-316-ADAPTER

Replacement 1/163/16 adapter for
connecting the air
sampler transfer line
to a Tedlar bag.

Ferrules for Sparge
Bottle Nut

Ferrules for Air Sample
Bag Adapter for FROG
Air Sampler

Sparge Line

Valve Assembly

Air Sampler Wand

1/16-3/16 Adapter

129

FROG-5000TM User’s Manual

APPENDIX I: AVAILABLE ACCESSORIES/SPARE PARTS
Item

Defiant Part #

Description

Air Sample Transfer
Line

DT-AS-TRAS-LINE

Air Sampler Coupler
Line

Replacement transfer
line for connecting air
sampler.

DT-AS-COUP

Replacement couple
line for connecting the
air sampler box to the
Frog.

DT-BTNF2040HD34-1

Rechargeable NiMH
Battery 10.8V, 6460
mAhr.

DT-DC-1

Ellvin (software)
communication cable
(All-in-one Data Cable)

Battery

Serial Data Cable

Power Supply

DT-FG5K-PS12V-1

Power Supply
12V 6.67A

Pump

DT-PU-1

130

Replacement air pump.

FROG-5000TM User’s Manual

APPENDIX I: AVAILABLE ACCESSORIES/SPARE PARTS
Item

Defiant Part #

Description

DT-FG-CFT-1

Replacement air
scrubber for cleaning
VOCs from sampled
air

DT-FG-CFTAB-1

Brass adapter for
connecting carbon
trap to FROG air
sample tube

DT-AS-FIL-1

Air Sampler
Particulate Filter and
Adapter

DT-AS-FIL-2

Air Sampler
Particulate Filter

Activated Carbon Filter
Tube

Activated Carbon Filter
Tube Adapter

Air Sampler Syringe
Filter and Adapter

Air Sampler Syringe
Filter

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FROG-5000TM User’s Manual

APPENDIX II: DETECTABLE CHEMICALS
Common Chemicals
Detectable by FROG 4000

CAS Number

IE (eV)

1,1-Dichloroethene

75-35-4

10.0

1,2,4-Trichlorobenzene

120-82-1

9.0

1,2-Dibromoethane

106-93-4

10.4

1,2-Dichlorobenzene

95-50-1

9.1

1,3-Dichlorobenzene

541-73-1

9.1

1,4-Dichlorobenzene

106-46-7

9.0

1,4-Dioxane

123-91-1

9.2

1-Propanol

71-23-8

10.2

2-Butanone (MEK)

78-93-3

9.5

2-Chloroethanol

107-07-3

10.5

2-Hexanone

591-78-6

9.4

2-Pentanone

107-87-9

9.4

2-Picoline

109-06-8

9.4

2-Propanol

67-63-0

10.2

4-Methyl-2-pentanone

108-10-1

9.3

Acetone

67-64-1

9.7

Acrolein

107-02-8

10.1

Allyl alcohol

107-18-6

9.6

Allyl chloride

107-05-1

10.1

Benzene

71-43-2

9.2

Benzyl chloride

100-44-7

9.1

Bromoacetone

598-31-2

9.7

Bromodichloromethane

75-27-4

10.6

Bromoform

75-25-2

10.5

Bromomethane

74-83-9

10.5

Carbon disulfide

75-15-0

10.1

Chlorobenzene

108-90-7

9.1

Chlorodibromomethane

124-48-1

10.6

Chloroethane
Chloroprene

75-00-3
126-99-8

10.0
8.8

133

FROG-5000TM User’s Manual

APPENDIX II: DETECTABLE CHEMICALS CONTINUED
Common Chemicals
Detectable by FROG 4000

CAS Number

IE (eV)

4170-30-3

9.7

Dibromomethane

74-95-3

10.5

Diethyl ether

60-29-7

9.5

Diisopropyl ether (DIPE)

108-20-3

9.2

Epichlorohydrin

106-89-8

10.6

Ethanol

64-17-5

10.6

Ethyl acetate

141-78-6

10.0

Ethyl tert butyl ether

637-92-3

9.4

Ethylbenzene

100-41-4

8.8

Ethylene oxide

75-21-8

10.6

Iodomethane

74-88-4

9.5

Isobutyl alcohol

78-83-1

10.1

Isopropylbenzene

98-82-8

8.8

Methacrylonitrile

126-98-7

10.3

Methyl methacrylate

80-62-6

9.7

Methyl tert-butyl ether

1634-04-4

9.2

m-Xylene

108-38-3

8.6

Naphthalene

91-20-3

8.1

n-Butanol

71-36-3

10.0

Nitrobenzene

98-95-3

9.9

n-Propylamine

107-10-8

8.8

o-Toluidine

95-53-4

7.4

o-Xylene

95-47-6

8.6

Propargyl alcohol

107-19-7

10.5

p-Xylene

106-42-3

8.5

Pyridine

110-86-1

9.3

Styrene

100-42-5

8.4

t-Butyl alcohol

75-65-0

10.3

Tetrachloroethene
Toluene

127-18-4
108-88-3

9.3
8.8

Crotonaldehyde

134

FROG-5000TM User’s Manual

APPENDIX II: DETECTABLE CHEMICALS CONTINUED
Common Chemicals
Detectable by FROG 4000

CAS Number

IE (eV)

trans-1,2-Dichloroethene

156-60-5

9.7

Trichloroethene

79-01-6

9.5

Vinyl acetate

108-05-4

9.2

Vinyl chloride
β-Propiolactone

75-01-4
57-57-8

10.0
9.7

NOTE: This list is by no means exhaustive. The FROG-5000TM
uses a 10.6eV lamp, so many analytes with ionization energies
below 10.6eV may be detectable with the instrument.

135

FROG-5000TM User’s Manual

Notes

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Notes

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FROG-5000TM User’s Manual

Notes

138

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Linearized                      : Yes
Author                          : Michele Harmon
Create Date                     : 2018:05:16 09:12:36-06:00
Keywords                        : 2018, Rev.3
Modify Date                     : 2019:05:06 08:41:37-06:00
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Producer                        : Microsoft® Publisher 2016
Format                          : application/pdf
Title                           : Defiant FROG-5000 Chemical Analysis System User’s Manual
Creator                         : Michele Harmon
Description                     : Defiant FROG-5000 Manual
Subject                         : 2018 Rev.3
Creator Tool                    : Microsoft® Publisher 2016
Metadata Date                   : 2019:05:06 08:41:37-06:00
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Defiant FROG 5000 Chemical Analysis System User’s Manual

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