Nikon If Not Then Digital Sight Series

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Digital Cameras for Microscopy
CAMERA HEAD
CCD

2/3 in. square pixel CCD; Total number of pixels:
1.5 megapixels (effective 1.45 megapixels)
1280 x 1024, 640 x 512, 640 x 480, 320 x 240
Peltier Device, 10°
C below ambient temperature (max.)
Equivalent to ISO 800 (switchable sensitivity equivalent to
(recommended exposure index) ISO 400 to 8000)
A/D conversion
12-bit
Live display mode (DS-L2)
1280 x 1024 (max. 19 fps), 1280 x 720 (max. 24 fps),
640 x 480 (max. 32 fps), 320 x 240 (max. 48 fps)
*Display reduced or enlarged to SXGA/XGA.
Supports also 8 bit live display

2/3 in. high-density CCD; Total number of pixels: 5.24 megapixels (effective 5.07 megapixels)

Recordable pixels
CCD cooling device
ISO Sensitivity

2560 x 1920, 1280 x 960, 640 x 480

2560 x 1920 (max. 5.9 fps), 1280 x 960 (max. 12 fps),
ROI mode (max. 23 fps)
* Display reduced or enlarged to SXGA/XGA

2560 x 1920 (max. 3.8 fps), 1280 x 960 (max. 7.5 fps),
Center Scan (max. 15 fps)
* Display reduced or enlarged to SXGA/XGA

Live display mode (DS-U2)

1280 x 960 (max. 7.2 fps), 640 x 480 (max. 11.5 fps),
ROI mode (max. 22 fps)

1280 x 960 (max. 5.6 fps), 640 x 480 (max. 7.6 fps),
Center Scan (max. 15 fps)

1/1000 to 60 sec

1/1000 to 600 sec
91.0 (W) x 76.0 (D) x 41.0 (H) mm
Approx. 290g

Lens mount
Exposure time
Dimensions
Weight
System composition
Optional accessories

Peltier Device, 20°
C below ambient temperature (max.)
Equivalent to ISO 64 (Can be varied between ISO 32-1250 equivalent)

1280 x 1024 (max. 19 fps 8bit mode/Max 10fps 12bit mode),
645 x 512 (max. 19 fps), 640 x 480 (max. 32 fps),
320 x 240 (max. 48 fps), ROI mode (max. 48 fps)
*Supports also 8 bit live display
C-mount
1/1000 to 600 sec
77.0 (W) x 76.0 (D) x 44.0 (H) mm
Approx. 290g
Camera Cable (3m)
For wide field of view observations 0.7x Relay lens

Approx. 260g

CAMERA HEAD
CCD
Recordable pixels
CCD cooling device
ISO Sensitivity
(recommended exposure index)

A/D conversion
Live display mode (DS-L2)

Live display mode (DS-U2)
Lens mount
Exposure time
Dimensions
Weight
System composition
Optional accessories

2/3 in. square pixel CCD; Total number of pixels:
1.5 megapixels (effective 1.45 megapixels)
1280x1024, 640 x 512, 320 x 256 (4076 x 3116,
3840 x 3072, 1920 x 1536 with DS-U2)
Peltier Device, 10°
C below ambient temperature (max.)

1/1.8 in. high-density CCD: Total number of pixels: 2.11 megapixels (effective 2.01 megapixels)

Equivalent to ISO 200
(switchable sensitivity equivalent 100 to 2000)
12-bit
1280 x 1024 (max. 19 fps), 640 x 480 (max. 32 fps),
ROI mode (max.23 fps)
*Display reduced or enlarged to SXGA/XGA
1280 x 1024 (max. 19 fps), 640 x 512 (max. 19 fps),
320x256 (max. 19 fps), ROI mode (max. 28 fps)
C-mount
1/1000 to 600sec, 1/1000 to 60sec (pixel-shifting mode)
77.0 (W) x 76.0(D) x 44.0(H) mm
Approx. 350g
Camera Cable (3m)
For wide field of view observations 0.7x Relay lens

Equivalent to ISO100 (Can be varied
between ISO 50-2000 equivalent)

1600 x 1200, 800 x 600, 400 x 300
Peltier Device, 20°
C below ambient
temperature (max.)
Equivalent to ISO350 (Can be varied between ISO 160-6400 equivalent)

1600 x 1200 (max. 15 fps), 800 x 600 (max. 20 fps), 800 x 560 (max. 30 fps), Center Scan (max. 30 fps)
* Display reduced or enlarged to SXGA/XGA
1600 x 1200 (max. 4.6 fps), 800 x 600
(max. 17 fps), Center Scan (max. 20 fps)

1600 x 1200 (max. 10 fps), 800 x 600 (max. 20 fps), Center Scan (max. 28 fps)

1/1000 to 60 sec
91.0 (W) x 76.0 (D) x 41.0 (H) mm
Approx. 260g

1/1000 to 600 sec

1/1000 to 60 sec

Approx. 290g

Approx. 260g

For wide field of view observations 0.55x Relay lens

CONTROL UNIT
Exposure control
Exposure correction
Digital zoom
Interval shooting
Exposure metering
Exposure metering range
White balance
Image adjustments
Storage format
Interface
Power supply
Power consumption
Dimensions
Weight
Operating environment
System composition
Networking
LCD monitor
External monitor output
Storage media
Direct printing
Optional accessories

Program AE, Shutter-priority AE, Focus AE, Manual with AE lock function
Correction range: ±2.0, Step: 1/3
Up to 16x (8 steps)
10 sec. - 6 hr. intervals
Average metering, Peak hold metering
Position/size adjustable
Set method, Color balance adjustable
Gamma correction, shading adjustment, black level adjustment, hue adjustment, color saturation adjustment
BMP, JPEG (4-step compression), TIFF, RAW (only with monochrome cameras)
USB device port x 1 (Mass Strage Class support, computer control connection, PictBridge)
USB host port x 2 (USB mouse, USB memory stick, USB keyboard, microscope connection)
AC100-240V 50/60Hz
70VA
230 (W) x 64.5 (D) x 200 (H) mm
CCU: approx. 1400g / AC Adapter: approx. 350g
0-40°
C, 60% RH max. (without condensation)
AC adapter, Power cord, USB memory stick 128MB、Mouse
Ethernet (10/100Base-TX), DHCP compatible, HTTP, TELNET or FTP server, FTP client
8.4-in. TFT color LCD XGA (1024 x 768, 60Hz)
DVI-I (Digital: Conforms to DVI 1.0/Analog: 0.7 Vpp (75Ω) SXGA/XGA
CompactFlashTM card (Type I, Type II), USB memory stick, Microdrive
PictBridge printer (sold separately)
DS remote controller, microscope control license

13 steps
5 to 2400%
5 sec. - 12 hr. intervals

BMP, TIFF, JPEG, JPEG2000
USB device port x 1 (computer control connection),
USB host port x1 (microscope connection)
35VA
193 (W) x 195 (D) x35 (H) mm
CCU: approx. 1200g / AC Adapter: approx. 350g
AC Adapter, Power cord, NIS-Elements F package

Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. February 2008 ©2004/2005/2006/2008 NIKON CORPORATION
The products detailed in the brochure are controlled by the Japanese Foreign Exchange and Foreign Trade Law and the International Export Control Regime. If there is a possibility that they may be utilized for the development of
weapons of mass destruction, etc., they shall not be exported without authorization from the government.

NIKON CORPORATION
6-3, Nishiohi 1-chome, Shinagawa-ku,
Tokyo 140-8601, Japan
phone: +81-3-3773-8973 fax: +81-3-3773-8986
http://www.nikon-instruments.jp/eng/

NIKON INSTRUMENTS INC.
1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.
phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A. only)
fax: +1-631-547-0306
http://www.nikoninstruments.com/
NIKON INSTRUMENTS EUROPE B.V.
Laan van Kronenburg 2, 1183 AS Amstelveen, The Netherlands
phone: +31-20-44-96-222 fax: +31-20-44-96-298
http://www.nikoninstruments.eu/
NIKON INSTRUMENTS (SHANGHAI) CO., LTD.
CHINA phone: +86-21-5836-0050 fax: +86-21-5836-0030
(Beijing branch) phone: +86-10-5869-2255 fax: +86-10-5869-2277
(Guangzhou branch) phone: +86-20-3882-0552 fax: +86-20-3882-0580

Printed in Japan (0802-XX) Am/M

NIKON SINGAPORE PTE LTD
SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668
NIKON MALAYSIA SDN. BHD.
MALAYSIA phone: +60-3-7809-3688 fax: +60-3-7809-3633
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KOREA phone: +82-2-2186-8410 fax: +82-2-555-4415
NIKON CANADA INC.
CANADA phone: +1-905-625-9910 fax: +1-905-625-0103
NIKON FRANCE S.A.S.
FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33
NIKON GMBH
GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322
NIKON INSTRUMENTS S.p.A.
ITALY phone: +39-55-3009601 fax: +39-55-300993
NIKON AG
SWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861

Code No. 2CE-MRGH-4

NIKON UK LTD.
UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584
NIKON GMBH AUSTRIA
AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40
NIKON BELUX
BELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45

DIGITAL CAMERA SYSTEM FOR MICROSCOPY

Over 12 million pixels for
high-definition color images
COLOR COOLED

High resolution

Build an imaging system
optimized for your application
With 7 cameras, you can select the optimum camera from an extensive
lineup to suit a wide range of applications for microscopic imaging.
The Digital Sight (DS) camera system allows you to freely combine
camera heads with stand-alone or PC-based control units.
The DS series offers outstanding functionality and a wide range of
incorporated functions, all in a compact design. You can build an optimal
microscopic digital imaging system for any biomedical or industrial application,
from documentation to advanced image processing and analysis.

Super-high output pixel

True color

COLOR CAMERAS

DIGITAL CAMERA SYSTEM FOR MICROSCOPY

With 12.7 megapixel output, true color reproduction, low noise, and wide dynamic
range, this color documentation camera is an ideal choice. Superb spatial resolution
and accurate color rendition make the DS-Ri1 the camera of choice for many
research, development, and analytical applications.
* Nikon recommends using the DS-U2 control unit.

12.7-megapixel high-definition images

Smooth live image display

New, high-precision pixel shift technology brings high resolution to the
CCD. The camera features super-high pixel output of 12.7 megapixels
and high resolution at over 2,200 TV line.

Accelerated data transfer offers a high maximum frame rate of 19 fps/
1.4 M. Live images are displayed and updated smoothly, so focusing
from a monitor is extremely simple and natural.

erate

Fram
Low

e

Imag

erate

Fram
High

e

Imag

Better fluorescence images

True color for samples

The DS-Ri1 uses the Peltier
cooling system to maintain the
CCD at 10°C below ambient
temperature, effectively
minimizing thermal noise
contributions.

Nikon’s newly developed color matching logic achieves unprecedented
color reproducibility. You can develop hue and saturation conversion
matrices optimized for the characteristic colors of your microscope
samples, and faithfully record the color of dyed samples. The ability to
capture the colors observed with the microscope enables highprecision color analysis in all fields of medicine and industry.

Configuration of DS-Fi1-L2

2

Configuration of DS-Ri1-U2 with AZ100

3

COLOR CAMERAS

High-definition color camera head

Cooled camera head
for high-definition color images

COLOR
COLOR COOLED

High resolution

True color
High resolution

The DS-Fi1 is the industry standard for documentation applications in industrial
and biomedical fields. With high performance in resolution, frame rate, and color
fidelity, the DS-Fi1 fits a very wide range of imaging applications.

5.0-megapixel color CCD

True color rendition of samples

Small pixel size and a field-of-view of 2,560 x 1,920 pixels allows you
to acquire color images at very high resolution.

Nikon’s industry-leading imaging technology achieves the best true
color image possible, duplicating exactly what is seen by the
microscope.

Smooth live image display

High red sensitivity

With a frame rate of 12 frames per second, live images are displayed
and updated smoothly, so focusing from a monitor is a simple task,
greatly increasing the speed of inspection, measurement tasks, and
acquisition.

A newly designed IR cut filter eliminates unwanted infrared
contributions from halogen light sources, while maintaining excellent
red color rendition for outstanding color reproduction.

Better fluorescence images
The DS-5Mc uses the Peltier cooling mechanism to cool the CCD to 20°C below the ambient
temperature. When capturing fluorescence images where long exposures are required,
thermal background noise is suppressed, enabling you to obtain high-contrast images.
Configuration of
DS-5Mc-L2
with ECLIPSE 80i

5.0-megapixel color CCD
High-resolution images with a maximum size of 2,560 x 1,920 pixels allow you to capture
even the microstructures of samples faithfully.

Support for a wide range of applications
The DS-Fi1 is a proven, cost-effective camera for a wide variety of applications including brightfield, phase contrast, and differential interference
contrast (DIC).

High-speed color camera head
COLOR

High speed

Smooth live image display
The DS-2Mv is equipped with a 2.0-megapixel color CCD. SXGA live motion images can be
displayed with a high speed of 15 fps (maximum 30 fps), and focusing is also performed
easily.

Configuration of
DS-2Mv-L2
with SMZ1000

High sensitivity
With high sensitivity and a wide dynamic range, you can obtain clear and sharp images.

Configuration of DS-Fi1-U2 with LV100

4

5

High-sensitivity cooled monochrome
camera head

Linearity, a quantitivity index, has been improved to >98%. This,
together with reduced noise, assures comparable, quantitative image
data collection over a wide exposure range.

MONO COOLED

Qi1 exposure time and output image intensity

Quantitative

The DS-Qi1 is the definitive camera for time-lapse fluorescence observation. By
combining low-noise electronics and a high-quantum efficiency detector, the DS-Qi1
can capture a wide dynamic range of intensities while maintaining quantitative
linearity. Added features such as a fast analog-to-digital converter (ADC), very low
read noise, and programmable gain control make the DS-Qi1 an ideal detector for
fluorescence imaging applications.

Output image intensity

High sensitivity

Configuration of DS-Qi1-U2
with biological inverted microscope

MONOCHROME CAMERAS

High speed

Reliable quantitative analysis

4000

3000

2000

1000

0
0

1

2

3

4

5

6

Exposure time (sec)

Cooled camera head
for high-speed monochrome images
MONO COOLED

High speed

Faint fluorescence detection

Clear, high-contrast images

The high-sensitivity CCD, which has outstanding quantum efficiency
(>60% at 500nm), combined with low read noise, allows the capture
of even low-light fluorescence signals. Intensity and time of
fluorescence excitation can therefore be minimized to reduce
photobleaching.

The Peltier cooling mechanism cools the CCD down to 10˚C below
ambient temperature, reducing the average dark current to 0.7e-/
pixel/s. Also, the readout noise is reduced to 8e- rms with the newly
developed CCD drive circuit. Thus, clear, high-contrast fluorescence
images can be captured at a dynamic range of over 2000:1.

High-speed camera head
for monochrome images
MONO

Quantum efficiency

High speed

0.9
0.8

Better fluorescence images

0.7
0.6

The DS-2MBWc uses the Peltier cooling mechanism to cool the CCD
to 20°C below the ambient temperature. When capturing fluorescence
images where long exposures are required, thermal background
noise is suppressed, enabling you to obtain high-contrast images.

0.5
0.4
0.3
0.2
0.1
0
400

500

600

700

800

900

1000

Smooth live image display
A high frame rate of up to 32fps with 640 x 480 pixel image size (2 x 2
binning) is possible, using a fast 36MHZ analog-to-digital converter.
As images can be displayed near real time, focusing and positioning
is easy, even in fluorescence . The CCD can also be binned and/or
subregioned. Thus, the duration of a specimen’s exposure to the
excitation light can be reduced, resulting in less photobleaching.

6

Wavelength characteristic of DS-2MBWc/DS-2MBW
1.0

Relative response

Relative response

1.0

0.8

0.6
0.4

0.2

0
400

500

600

700

800

900

1000

Wavelength（nm）

Smooth live image display
The cameras are equipped with a 2.0-megapixel monochrome CCD.
Live motion images can be displayed with a high speed of 15 fps
(maximum 30 fps), and focusing is also easy to perform.

7

Choose from two types of control units.
Select a stand-alone unit that does not require a computer, or a PC-based unit for integration with image processing software.

Save data without a PC

Stand alone control unit

No PC required. Simple operation with a mouse.
Live observation and camera control are
possible on the large built-in LCD monitor.

• USB memory stick
• CompactFlash card
• Mass storage class: Data on a CF card inserted into the DS-L2 can be
read at high speed using a PC connected via the USB 2.0 interface.
• Network: Image files can be transferred (stored) at high speed to
any connected server using the FTP client function. This enables
you to capture images without worrying about storage space.

Supports large screen output
A digital output port conforming to DVI 1.0 is provided. This enables
the display of high-definition images on a large external display with
no loss of image quality, as only digital imaging can do. Applications
include observation by large numbers of people at conferences.

A large 8.4-inch high-definition LCD monitor (XGA) is built into the
controller. Image capture can begin simply by turning on power. No
PC required!

On-screen GUI
All operations can be performed by clicking on-screen menus. Users
can concentrate on observing live images thanks to simple image
capture while viewing the monitor.

Crosshairs

Measurement

Area

θ

Scene mode enabling optimal
image capture with a single click

Supports direct printing (PictBridge)

Anyone can easily configure optimal image capture settings simply by
selecting the observation method, such as brightfield or darkfield, and
the type of sample. In the Biological Scene Mode, there is even a
mode optimized for HE and ELA (Enzyme labeled antibody) stained
specimens for use with medical specimens that require subtle color
reproduction. There are also seven user-customizable modes.

No computer is required to print out images with a single click. Simply
connect the unit’s USB port to a printer supporting the PictBridge
standard.

Shortcut menu for greater ease of use
Functions that are used frequently are displayed as buttons. This
enables operation without obstructing the display of captured images.
The buttons can also be customized.

Distance between two points

CONTROL UNITS

High expandability

Built-in LCD display

A wide variety of tools

Angle

Distance between
two points

Point-to-line distance

Distance between
circle centers

Pitch

Grid lines

Scale

Scale display

Crosshairs

1

XY scales

Superimpose

2

3

Count marker

XY measurement*

*Measurement of the distance between two points in the x and y axis directions.

Exceptional color representation
Nikon has created a system producing life-like color rendition by using
newly-developed image processing technology, thereby enabling true
natural color representation, so what is acquired as an image is
perfectly matched to what you see in the microscope. The controller
has seven default gamma (TONE) correction presets and another
seven that can be customized by the user. It also allows the user to
create an original look-up table (LUT), all features to satisfy
exceptional color rendition for a variety of microscope applications.

Time-lapse recording
Image acquisition over periods of time can be performed using the DS-L2.

Biological scene mode

Industrial scene mode

Other

An example of the shortcut menu (above: Short, below: Large)

The screen can be split in
half to display a still image
alongside a live image. This is
handy when you want to
compare and contrast a sample
image that serves as a
reference with a live image.

Using NIS-Elements imaging software
(→ page 10), you can perform image
acquisition, processing, and analysis
with integrated control of the camera
and microscope peripherals.
Captured image

8

PC control-based control unit

Two-screen split display

Live image

Two USB ports

Features a USB 2.0 device port that can be easily connected with a PC. The USB 2.0 device port enables the
transfer of image files to a PC at twice the speed of the previous model.

A mouse, keyboard, USB memory stick, or other USB device can be
simultaneously connected without the need for a USB hub.

Sophisticated thin and compact design.

9

Software that offers a total solution
for microscope imaging

SYSTEM DIAGRAM
Standard set

NIS-Elements, imaging software developed by Nikon, is an integrated platform for control
of image acquisition and document data management using the DS-series of cameras.
NIS-Elements supports multi-dimensional imaging tasks, including control of integrated
motorized microscope components and peripheral devices. There are 3 packages
available to match your needs and applications:
NIS-Elements

Ar Advanced Research
NIS-Elements AR is optimized for advanced
research applications. It features fully
automated acquisition and device control
through full 6D (X, Y, Z,λ (Wavelength),
Time, Multipoint) image acquisition and
analysis.

NIS-Elements

Br Basic Research
NIS-Elements BR is suited for standard
research applications. It features acquisition
and device control through 4D (up to four
dimensions can be selected from X, Y, Z,λ
(Wavelength), Time, Multipoint) acquisition.

Option

Projector
Large-screen monitor (External output)
(External output)

USB
memory stick

Network server
PC
PictBridge printer
(WEB, FTP, telnet) (Mass storage class) (Direct printing)

USB mouse

NIS-Elements

DVI-I
LAN (Ethernet)
USB (D)
USB (H)
USB (H)

D Documentation
NIS-Elements D supports color
documentation requirements in bio-research,
clinical and industrial applications, with basic
measuring and reporting capabilities.

USB keyboard
Microscope
(License card required)
Compact flash card, microdrive
Dedicated remote controller/strobe, etc.

* The DS-U2 includes NIS-Elements F package,
making basic image capture a snap.

Universal-type AC power adapter

Camera control unit

Camera head DS series

Dedicated camera cable (3m)

Various convenient plug-ins are available
for advanced imaging and analysis capabilities
Multidimensional Capturing

3D/2D Real Time Deconvolution

NIS-Elements can combine X, Y, Z,λ
(wavelength), Time and Multi points
within one integrated platform for
multidimensional imaging (depends on
the capability of the software). All
combinations of multidimensional
images can be linked together in one
ND2 file sequence using an efficient
workflow and intuitive GUI.

Haze and blur of the fluorescence image can be eliminated from the
captured 3D image or from the 2D live preview image. (Separate
plug-in for 3D and 2DRT) (Ar package)

USB（D）

Windows XP
USB（H）

Universal-type AC power adapter

Camera control unit

Camera head DS series

Dedicated camera cable (3m)

Extended Depth of Focus
Database

You can easily join (stitch) adjacent image fields together into a single,
high-resolution result image by manually moving the microscope stage,
or with optional modules, do it automatically using a motorized stage.

195

200
230

64.5

193

35

Large Image

Using the Object Counting module, get real time analysis of
morphometric features of objects. Apply thresholds to define objects,
and classify objects by a range of
morphometrics features all from a
single, easy to use graphic user
interface (GUI).

76

Object Counting

91

76

77

77

41

DIMENSIONS

44

NIS-Elements has a powerful image database module that supports
image and meta data. Various databases & tables can easily be
created and images can be
saved to the database via one
simple mouse-click. Filtering,
sorting and multiple grouping
are also available according to
the database field given for
each image.

76

With the Extended Depth of Focus (EDF) plug-in, images that have
been captured in a different Z-axis can be used to create an all-infocus image. Also, it is possible to create stereovision images & 3D
surface images to achieve virtual 3D imaging.

44

IMAGING SOFTWARE

Microscope

Visit www.nis-elements.com  for more detailed information

10

11



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