Exactive 062 Quadrupole Cleaning Procedure Rev0

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Factory: BREMEN Date: 23-Jul-12 #: Exactive- 062

Model: Q Exactive Product Line: Hybrid-LSMS

Instrument: Exactive Series Revision Level: 0

Author: Andreas Boegehold

Quadrupole Cleaning Procedure

In some special cases the quadrupole on the Q Exactive can become

contaminated over time.

The contamination will appear on the TK lens and the front of the

Quadrupole rods. Typically only two opposing rods should show the

contamination.

Under good lighting conditions the contamination can be found by

visual inspection, but it is hard to spot. Thorough cleaning as described

in the following procedure is recommended for all 4 rods. The cleaning

should only be performed by authorized FSEs.

The picture below shows the contamination and where it will be

located.

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Contamination on one of the quadrupole rods shown on a disassembled quadrupole assembly (disassembled for illustration

purposes only!). It is on the half of the quadrupole facing the TK lens.

1. Taking out the quadrupole

- Switch off the instrument and remove the covers

- Remove the quad electronics by loosening the 8 screws, pulling off the lid and removing the

power and SPI bus cables. Then loosen the 4 screws that secure the unit in place.

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- Loosen the screws on the CLT-RF board and swing it out of the way.

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- Loosen the 4 screws on the CLT flange and pull out the flange with the CLT.

- Loosen the 2 screws on the transfer octapole and disconnect the wires. Remove the octapole

from its holder.

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- Loosen the screws on top of the vacuum chamber and remove the lid.

- Disconnect the wires to the IOS RF feedthrough (split lens, quad exit lens and RF2) on the

vacuum chamber (the other side is attached to the assembly)

- Loosen the 2 screws on the quadrupole retainer assembly.

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- Firmly grip the quadrupole assembly and slide it to the left (the retainer should move, if it is

stuck, gently lift it upwards until free and proceed.). Gently tilt the quadrupole until the rods

are clear of the vacuum chamber wall and pull the quadrupole out of the chamber.

- When putting the quadrupole assembly down, take care to not place it on one of the

connector bolts for the RF, as these are directly connected to the rods. Support the end that is

not inside the retainer with something, e.g. a screwdriver handle (place it under the shell, not

under the rods, as seen in the picture below).

1. Slide

2. Lift

Be careful not to

damage the rods

while lifting.

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2. Removal of the retainer parts and connectors

- Each quad carries two serial numbers. Use one serial number of the two quad shells „V0000“

as reference for the orientation: Write down one serial number and its position (entrance/exit

and upper/lower). To not mix up the plastic rings, put the one that is already loose into the

TK lens assembly.

- Remove the Retainer from the Quad by loosening the two screws at the side, and the 4 screws

holding the wires. Put the other plastic ring into the retainer, if it came out.

Serial

Number

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- Remove the rods for the supply wires carefully (turn anti-clockwise):

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3. Cleaning the quadrupole rods

Required accessories:

Nylon-covered brush (e.g. from TSQ cleaning kit, PN 70111-62112)

Self made holder (e.g. from 2mm wire): Nitrogen blow-out tool (see below):

The nitrogen blow-out tool is a 6mm to 3.2mm (1/8 inch) OD adapter fitting (e.g.

SMC KJH23-06) and a short piece of 3.2mm tubing. These can be attached to the 6 mm tubing

that supplies the instrument to create a higher pressure.

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Procedure

- Prepare a solution of 1% Liquinox (or comparable detergent) in water.

- Scrub the quad from both sides for 2 minutes each.

- Use toothbrush to clean the ends of the quad rods.

- Thoroughly flush with tap water (ideally demineralized, otherwise rinse with some deionized

water afterwards.

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- Wash off the water with pure (LC grade or better) methanol

(LC grade Ethanol or Isopropanol are also acceptable). Use

the holder to fully submerge in Methanol. A 500 mL

graduated cylinder with 350 mL of methanol works fine.

- Blow off the methanol with nitrogen using the reducing

adapter. Do this immediately after removing from methanol

to prevent any drying marks.

Pay special attention to vent holes, as residual solvents will

considerably extend the pumping time afterwards.

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4. Cleaning the split lens

- Use a Micromesh 6000 swab to rub off the spot on the split lens.

- Use a lint-free swab with 50:50 methanol:water to wipe off the lens.

- Dry with nitrogen.

- Check carefully for residual lint.

5. Cleaning the TK lens

- Disconnect the wires going to the bent flatapole.

- Remove the screw and Pin holding the bent flatapole:

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- Take out the bent flatapole.

- Take note of the wiring for the inner and outer

TK lens and unplug the two yellow wires.

- Loosen the holding screw on the TK lens

assembly (yellow arrow) and pull the assembly

out to the left. If it cannot be moved without

force, wait some more time to let the

instrument cool down further.

- Remove any contaminations like done before

on the split lens.

- Blow dry with nitrogen

- Carefully check for remaining lint on the

lenses.

6. Inspection

- Inspect the quadrupole for

leftover dirt or any damage taken

during the procedure. Repeat

after reassembly.

- Especially check for cracks on

the spacers holding the rods.

Cracks like those shown in the

picture on the right mean that the

quadrupole assembly is broken

and needs to be replaced

7. Reassembly

- Reinstall the TK lens assembly and tighten the holder screw.

- Connect the two yellow wires for the inner and outer TK lens.

- Install the bent flatapole (hint: take out the screw and washer and put them on the holder pin.

Now hold the pin and screw, insert it into the thread and let the screw drop into place and turn

it until it catches onto the thread before removing the pin). Now fix the bent flatapole in place

with the pin and tighten the screw (do not use too much force as this will push the bent

flatapole out of alignment).

- Go through the steps under 2 in reverse order to reassemble the quad.

- Do not overtighten the bolts on the quad rods. This might bend the quadrupole rods or

crack the isolators which hold them in the shell.

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- Take care to insert the quad in the same orientation

it had before. Refer to the notes on serial numbers

from the beginning of the procedure.

- Check that the split lens assembly is aligned correctly

(guidance pin) in the quadrupole retainer piece.

- To mount the retainer piece, the screws go into the slit

between the two shells as shown in the picture on the

right. Hold the retainer in your hands with the Quad

installed then insert the screws and metal springs. If

you put it down on a table, it will not be possible to get

the screws installed properly.

- Go through the steps in 1 in reverse order to

reassemble the remaining parts.

8. Calibration

- After performing the vacuum bakeout, the system will need to be fully calibrated in “Advanced

Mode” to properly adjust the ion transfer settings to the clean quadrupole. Any Manual

Settings in the quad tweak parameters need to be removed.