Springer Protocols Manuscript Instructions MIMB

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Springer Protocols Manuscript Instructions

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Length

Illustrations

Book Covers

Formatting

Permissions

References

Submission

Dos and Don’ts

Video Protocols

Contact Your Publishing Editor

Sample Permission Request Letter – Chapter Authors

Sample Permission Request Letter – Authored Books

Consent to Publish Form

LENGTH

For editors: The length of your text is specified in your contract. This page count includes

references, tables and figure legends. Please be sure to check your page count as you write as it is

very important to keep within the specified limit, and distribute length information to your chapter

authors.

For authors: Please consult with your editor for the appropriate length of your chapter, and number

of figures. Please submit your chapter to the volume editor and not directly to Humana Press.

ILLUSTRATIONS

For authors and editors:

If you can submit your illustrations in an electronic form, please submit the original graphic files. Do

not insert figures into your Word document. Humana Press prefers that files be submitted in TIFF

or EPS format, with at least 300 dpi resolution. PDF, JPG, Excel, and PowerPoint files may also be

acceptable. Please check with your editor if you have questions regarding specific figures.

Humana Press prefers that art be submitted as electronic files rather than as hard copies; however,

scanned images may not be of sufficient quality for reproduction, and authors/editors should expect

to submit hard copies of scanned art along with their manuscript. Please scan art at 600 dpi.

Remember when considering figures that they will probably be reduced to fit onto a book page, so

pay particular attention to making labels highly legible and not too small. You may want to photocopy

your figures to the expected final size as a test to determine legibility. The usable page size of the

book will usually be about 4.5 x 7.5 inches. Most figures (including their caption) will occupy half a

page. Labels should be in a sans-serif font, preferably Helvetica. When at final reduction the size

should be no smaller than 8 point.

Please ensure that your figures are clearly labeled. Number tables separately.

Please submit your images to Humana Press in full color when possible. All electronic versions of the

book will publish in full color, including the e-book versions and the chapters available on our online

book database, Springer Link (Springerlink.com).

While the cost of reproducing color figures usually prohibits Humana Press from printing color

images in our print books, there is a certain amount of color that we can print in each book gratis.

This is a number dependent upon the overall size of the submitted manuscript, but usually this

includes about 12-20 images. All images will be printed in color in the electronic versions of the book.

Please do not expect artwork to be printed in color unless you have consulted with your editor or it is

stated in your contract. Color artwork can be included without limit in the electronic version of the

book; however, submitted artwork in this case must also be understandable in grayscale, as the

same file will be used to produce the print and electronic versions.

BOOK COVER

If there is an image appearing in the book that you would like to use for the cover, please note

this when submitting the manuscript. While all of our book covers are designed by our Art

Department, we like to use an image appearing in the book. Please make sure that the image

selected does not require permissions and is acceptable to the chapter author who submitted the

figure. While we try to ensure the image you select is used, we cannot guarantee that your

image will be used for the cover.

FORMATTING

For authors and editors:

While we find it the most productive for authors to work with software tools with which they are

most comfortable, we prefer that manuscripts are submitted in Microsoft Word. This makes the

review and production of the book move much quicker. If you need to work in a program that is

not Microsoft Word, please contact us so we can ensure we have the program on hand during

submission.

Please ensure that all material, including figure and table captions, is double-spaced throughout.

There should be a left and right hand margin of at least one inch on all pages.

Chapter Guidelines:

The purpose of this series is to provide individual volumes that describe detailed practical

procedures for a particular subject area. Although the research literature contains descriptions of

practical procedures, extensive practical detail is not always presented, and, more particularly, an

idea of the problems that can be encountered and how they are identified and overcome is rarely

included. This series aims to address these deficiencies. Each chapter in a volume of this series

should therefore be one specific method, written such that a competent scientist who is unfamiliar

with the method can carry out the technique successfully at the first attempt by simply following

the detailed practical procedures that you have described. The success of the series is, in large

part, due to the usefulness of the "Notes" section. Chapters tend to average 10-15 double-spaced

typed pages, but can be longer or shorter as necessary. Unless your editor has specifically

requested that you write a review chapter, each contribution should conform to the following

format as closely as possible. (A Sample Chapter is included as a reference).

Please arrange your chapter text file in the following order:

i. Summary/Abstract

In one or two paragraphs, please write an overview of the method described.

ii. Key Words

Please include 5-10 key words for referencing by electronic databases and search engines.

1. Introduction

This section should contain a summary of, and the outline of any theory to, the method that you’re

are describing. It should also outline the major procedures involved in the protocol.

2. Materials

This section should list the composition of all buffers, media, solutions, and specialist equipment

etc., that are necessary for carrying out the method described in section 3. Suppliers aren’t

needed for routine reagents (the reader will use his/her own local supplier) and catalogue

numbers aren’t required at all for reagents. All buffers, solutions and media should be presented

in the same format i.e. name, colon then composition on one continuous line, with components

separated by commas not semi-colons.

3. Methods

This is the main section and should explain in detail the individual steps necessary to carry out

the technique. Where possible, please simply list the steps in numerical order. For techniques

that comprise a number of separate major procedures, please indicate these separate

procedures in the introduction, and then subdivide section 3 into subheadings to cover each

procedure (3.1, 3.2 etc; please avoid any further subdivision of these headings). The steps in

each subsection should then be numbered individually, renumbering from number one. Do take

great care to try to indicate any little "tricks" or nuances that help improve your method by

referring to relevant "notes" in section 4 (see below). This sort of information rarely gets into the

scientific literature. You may also find it useful to relate to some aspects of the theory in this

section indicating the purpose of some of the major steps by cross-referencing to an appropriate

“note”. Do not be tempted to get involved in the description of variations/alternatives to your

technique in this section: this can be done in the "Notes" section. Stick to the basic procedure

detailed in this section.

This section must be comprehensive. Do not send the reader away to find information for a

particular step in another reference. All relevant practical detail must be given in this section.

4. Notes

As we all know, even the simplest techniques go wrong from time to time. Would you therefore

indicate any major problems or faults that can occur with your technique? Try to indicate the

major sources of problems and how they can be identified and overcome. With reference to

related techniques, any variations of the technique that you have described should also be made

in this section, as well as--where relevant--an indication of the sensitivity of the method, timescale

for the singled technique, etc. This "Notes" section is a hallmark of this series and has been

singled out for praise by a number of reviewers. Please try and make this section as extensive as

possible by putting on paper all of your various experiences with the technique. Each ‘Note’

should be cross-referenced with the ‘Materials’ and ‘Methods’ sections, e.g. (see Note 1).

5. References

Arabic numbers should be used for text citations (set within parentheses at point of citation), and

they should be listed in numerical order in text, as well as in the reference section. Please be as

comprehensive as possible with the references.

Figure Captions

Table Captions

Tables -- Always to be included at the end of the document.

*Figures should be submitted as separate files

 Please include a cover page that includes the names, affiliations, and e-mail

address of the corresponding author on the first page of your chapter.

 In addition, please provide us with a “Running Head” (a “Running Head” is an

amended/shortned version of your chapter title that appears at the top of each

page in the final version of the book).

 Please make sure all figures are referenced in the text. If you would like a figure to appear at

a particular point in the book, write “[Fig X near here]” in the body of the text. Otherwise, all

figures will be placed on the page on which they are referenced.

 Please do not use fancy formatting in the manuscript unless for a good reason. Use one font

type only (preferably Times New Roman, or Palatino) bold, roman and italic only. Complex

formatting can confuse electronic typesetting processes, and we will be producing the book

to our own book designs.

 Please ensure all mathematical variables are in italics.

For details of nomenclature and details of chemical standards and so on please refer to a standard

guide such as the Chicago Manual of Style (Chicago University Press), or Scientific Style and

Format: The CBE Manual for authors, editors, and publishers (Cambridge University Press). The

important thing is to be consistent.

Humana Press will copyedit and typeset your book simultaneously, and return the files to you for

approval of changes, answering of queries, and making of any final revisions to the book. After

checking the copyediting, you return changes to us. Springer will enter the changes onto the file.

PERMISSIONS

For authors and editors: Please remember that if you intend to use any figure, table, or lengthy

text passage which has been previously published, you will need to formally request permission

to use the material from the original publisher. This also applies to material previously published

by Humana Press and Springer, and previously published material that is your own work. Please

keep in mind that it can sometimes take a while for requests to process, and you should start this

process as soon as possible. Grant letters for any necessary permissions should be submitted

to your book’s editor with your final chapter manuscript. If the figures have been redrawn so as to

be “significantly different” from the original, then no permission is needed.

All reproduced figures should be cited in the text by either using the wording supplied by the original

publisher, or by using a standard format such as “Reproduced from [Name Year] with permission

from [Publisher].” The full citation should be included in your References.

A sample permission letter is included on the last page for you to use as a model. Please make

sure that you label each letter granting permission with the correct figure number and your name

before submitting it to your book’s editor.

*Most Permissions can be obtained by going to the publisher’s website and filling out an

online form. This is usually the easiest and quickest way to obtain permissions!

Some publishers may ask for a fee (for example AAAS the publishers of Science always ask about

$25). According to your contract you are responsible for all permissions fees. Springer cannot cover

the cost of these permissions.

Please make sure that we know to which figure each permission letter refers, by writing the figure

number on the letter, or by preparing a figure log such as:

Figure No

5.1

Oxford University Press, New

York

reproduced by permission of

For editors: Please ensure that all permissions have been granted before submitting the finished

manuscript to Humana Press. Humana Press cannot start the production process until all

permissions have been granted.

REFERENCES AND BIBLIOGRAPHY

*Please note – the copyeditors will standardize the references (placing punctuation where

need be, adding italics, etc) so please use the below as a reference.

For authors and editors: Please ensure that the references in your book follow the Springer

house style as shown below:

Number

Type

Example

Journal article

Smith J, Jones M Jr, Houghton L et al (1999) Future of

health insurance. N Engl J Med 965:325–329

Inclusion of issue number

(optional)

Saunders DS (1976) The biological clock of insects.

Sci Am 234(2):114–121

Journal article with DOI (and

with page numbers)

Slifka MK, Whitton JL (2000) Clinical implications of

dysregulated cytokine production. J Mol Med 78:74–

80. doi:10.1007/s001090000086

Journal article by DOI (before

issue publication with page

numbers)

Slifka MK, Whitton JL (2000) Clinical implications of

dysregulated cytokine production. J Mol Med.

doi:10.1007/s001090000086

Article in electronic journal

by DOI (no paginated version)

Slifka MK, Whitton JL (2000) Clinical implications of

dysregulated cytokine production. Dig J Mol Med.

doi:10.1007/s801090000086

Journal issue with issue

editor

Smith J (ed) (1998) Rodent genes. Mod Genomics J

14(6):126–233

Journal issue with no issue

editor

Mod Genomics J (1998) Rodent genes. Mod

Genomics J 14(6):126–233

Book chapter

Brown B, Aaron M (2001) The politics of nature. In:

Smith J (ed) The rise of modern genomics, 3rd edn.

Wiley, New York

Book, authored

South J, Blass B (2001) The future of modern

genomics. Blackwell, London

10.

Book, edited

Smith J, Brown B (eds) (2001) The demise of modern

genomics. Blackwell, London

11.

Book, also showing a

translated edition [Either

edition may be listed first.]

Adorno TW (1966) Negative Dialektik. Suhrkamp,

Frankfurt. English edition: Adorno TW (1973) Negative

Dialectics (trans: Ashton EB). Routledge, London

12.

Chapter in a book in a series

without volume titles

Schmidt H (1989) Testing results. In: Hutzinger O (ed)

Handbook of environmental chemistry, vol 2E.

Springer, Heidelberg, p 111

13.

Chapter in a book in a series

with volume titles

Smith SE (1976) Neuromuscular blocking drugs in

man. In: Zaimis E (ed) Neuromuscular junction.

Handbook of experimental pharmacology, vol 42.

Springer, Heidelberg, pp 593–660

OnlineFirst chapter in a

series (without a volume

Saito Y, Hyuga H (2007) Rate equation approaches to

amplification of enantiomeric excess and chiral

Number

Type

Example

designation but with a DOI)

symmetry breaking. Top Curr Chem.

doi:10.1007/128_2006_108

15.

Proceedings as a book (in a

series and subseries)

Zowghi D et al (1996) A framework for reasoning about

requirements in evolution. In: Foo N, Goebel R (eds)

PRICAI'96: topics in artificial intelligence. 4th Pacific

Rim conference on artificial intelligence, Cairns,

August 1996. Lecture notes in computer science

(Lecture notes in artificial intelligence), vol 1114.

Springer, Heidelberg, p 157

16.

Proceedings with an editor

(without a publisher)

Aaron M (1999) The future of genomics. In: Williams H

(ed) Proceedings of the genomic researchers, Boston,

1999

17.

Proceedings without an

editor (without a publisher)

Chung S-T, Morris RL (1978) Isolation and

characterization of plasmid deoxyribonucleic acid from

Streptomyces fradiae. In: Abstracts of the 3rd

international symposium on the genetics of industrial

microorganisms, University of Wisconsin, Madison, 4–

9 June 1978

18.

Paper presented at a

conference

Chung S-T, Morris RL (1978) Isolation and

characterization of plasmid deoxyribonucleic acid from

Streptomyces fradiae. Paper presented at the 3rd

international symposium on the genetics of industrial

microorganisms, University of Wisconsin, Madison, 4–

9 June 1978

19.

Patent. Name and date of

patent are optional

Norman LO (1998) Lightning rods. US Patent

4,379,752, 9 Sept 1998

20.

Dissertation

Trent JW (1975) Experimental acute renal failure.

Dissertation, University of California

21.

Institutional author (book)

International Anatomical Nomenclature Committee

(1966) Nomina anatomica. Excerpta Medica,

Amsterdam

22.

Non-English, Latin alphabet

publication cited in an

English publication. NB: Use

the language of the primary

document, not that of the

reference for "vol" etc.!

Wolf GH, Lehman P-F (1976) Atlas der Anatomie, vol

4/3, 4th edn. Fischer, Berlin

23.

Non-Latin alphabet

publication cited in an

English publication. Optional

are the title of the publicaton

in the original language (and

alphabet) and an English

translation, which are placed

in parentheses when they are

present.

Marikhin VY, Myasnikova LP (1977)

Nadmolekulyarnaya struktura polimerov (The

supramolecular structure of polymers). Khimiya,

Leningrad

24.

In press

Major M et al (2007) Recent developments. In: Jones

W (ed) Surgery today. Springer, Dordrecht (in press)

25.

Online document

Doe J (1999) Title of subordinate document. In: The

dictionary of substances and their effects. Royal

Society of Chemistry. Available via DIALOG.

Number

Type

Example

http://www.rsc.org/dose/title of subordinate document.

Accessed 15 Jan 1999

26.

Online database

Healthwise Knowledgebase (1998) US Pharmacopeia,

Rockville. http://www.healthwise.org. Accessed 21

Sept 1998

27.

Supplementary

material/private homepage

Doe J (2000) Title of supplementary material.

http://www.privatehomepage.com. Accessed 22 Feb

2000

28.

University site

Doe J (1999) Title of preprint. http://www.uni-

heidelberg.de/mydata.html. Accessed 25 Dec 1999

29.

FTP site

Doe J (1999) Trivial HTTP, RFC2169.

ftp://ftp.isi.edu/in-notes/rfc2169.txt. Accessed 12 Nov

1999

30.

Organization site

ISSN International Centre (2006) The ISSN register.

http://www.issn.org. Accessed 20 Feb 2007

In the text:

Number all references in order of use throughout the chapter, and indicate the reference by

placing the number of the reference in bold, italicized parenthesis, like this (6). All references are

to be indicated like that (7) and should appear in numerical order throughout the text (8).

ENDNOTES: If using the citation program, Endnotes, please use the SpringerBasicNumber

bibliography format.

DOS AND DON’TS

For authors and editors:

Do:

 Be consistent with style.

 Place figure captions at the end of the file or in a separate file. Do not place figures or

captions "in place" on the manuscript page. Do not leave space for the figure in your

electronic manuscript.

 Double-space the manuscript using your word processor's line-spacing function. Do not

use hard returns or enters to double-space.

 Use two hyphens for a dash, with no space before or after them.

Don't:

 Don't try to make your document look like a typeset page.

 Don't type the letter "el" (l) when you mean the number "one" (1) or vice versa. Even if

the number cannot be distinguished from the letter in your printout or on your screen, it

will appear differently in typeset output.

 Don't interchange "zero" (0) and capital "oh" (O).

 Don't justify your text.

 Don’t indent paragraphs.

 If your book contains a lot of math, please consult with your editor to discuss submitting

author-formatted files.

 Don't number the Preface, which is an brief introduction to the book by the Editor(s), as a

chapter. The Preface is a part of what is called "front matter," and it is not numbered in

the Table of Contents.

SUBMISSION

For authors:

All key chapter authors must sign a Contributor’s Agreement (provided by your volume editor).

Please submit this signed form with your final manuscript to your volume editor.

For editors:

Please include a Table of Contents, a Preface, and/or Forward, as well as an alphabetical List of

Contributors (complete with affiliations) and a corresponding author list organized by chapter

(complete with addresses) for your book. Please also note if there is an image or figure in the text

that you would like to use for the book’s cover (all covers are designed by our Art Department.

PLEASE NOTE: We cannot always use the submitted artwork for your cover. While we always

try to use your suggestions, we reserve the final approval of the cover).

Please submit your book either on disk, USB stick or via ftp/DropBox (please contact your editor if

you’d like Springer to set up an ftp site). A hard copy is no longer necessary.

VIDEO PROTOCOLS

Springer has recently launched the video companion site Springer Videos (video.springer.com). If

you would like to film a short video to accompany your protocol chapter, please contact your

editor for more information. Information about formatting the videos can be found:

http://video.springer.com/LearnMore

CONTACT YOUR PUBLISHING EDITOR

Please consult with your publishing editor if you have any questions not answered here.

Sample Permission Request Letter –Chapter Authors

To: Copyright Permission Department

______________________________

______________________________ Date: _________

In my chapter tentatively entitled: ________________________________________________

that is to appear in the book: ____________________________________________________

edited by: ____________________________________________________________________

to be first published by Humana Press, part of Springer Science+Business Media, I would greatly

appreciate your permission to incorporate the material set forth below with world rights in all

languages, in this and any future editions, revisions, adaptations or translations, and in any other

form including, without limitation, mechanical & electronic storage on any carrier:

Title: ________________________________________________________________________

Author(s): ____________________________________________________________________

Year of publication: ____________________________________________________________

Material for which permission is requested:

_____________________________________________________________________________

Please provide preferred line for copyright notice/credit:

_____________________________________________________________________________

Two copies of this request are enclosed. Please return one and retain one for your records.

Signed:________________________________

Name:_________________________________

Address:

______________________________________

**SPRINGER IS A SIGNATORY OF THE STM GUIDELINES AGREEMENT**

………………………………………………………………………………………………

We hereby grant permission for use of the material as specified above.

By:_________________________________

Title:_______________________________

Sample Permission Request Letter – Authored Books

To: Copyright Permission Department

______________________________

______________________________ Date:__________________

I am preparing a manuscript for a Work tentatively entitled:

_____________________________________________________________________________

to be first published by Humana Press, part of Springer Science+Business Media. I would greatly

appreciate your permission to incorporate the material set forth below with world rights in all

languages, in this and any future editions, revisions, adaptations or translations, and in any other

form including, without limitation, mechanical & electronic storage on any carrier:

Title: ________________________________________________________________________

Author(s): ____________________________________________________________________

Year of publication: ____________________________________________________________

Material for which permission is requested:

_____________________________________________________________________________

Please provide preferred line for copyright notice/credit:

_____________________________________________________________________________

Two copies of this request are enclosed. Please return one and retain one for your records.

Signed:________________________________

Name:_________________________________

Address:

______________________________________

**SPRINGER IS A SIGNATORY OF THE STM GUIDELINES AGREEMENT**

………………………………………………………………………………………………

We hereby grant permission for use of the material as specified above.

By:_________________________________

Title:_______________________________

http://www.springer.com/series/7651

Springer Protocols Manuscript Instructions MIMB

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