RESEARCH INSTRUMENTS 670854 RI Witness Sperm Prep Reader User Manual

RESEARCH INSTRUMENTS LTD RI Witness Sperm Prep Reader

User Manual

User Manual
RI Witness™
Document 6-70-121UM, Issue 13, 26th January 2015, MRF 1636
RESEARCH INSTRUMENTS LTD
Bickland Industrial Park, Falmouth, Cornwall TR11 4TA, UK
t: +44 (0) 1326 372 753 | f: +44 (0) 1326 378 783 |e: sales@research-instruments.com
www.research-instruments.com
CONTENTS
 
 
Intended Use 2
Medical Device Component 2
Applicable Part Numbers
2
Compability 3
Installaon 3
 
GuidanceandManufacturer’sDeclaraon(Part15ofFCC)
—ElectromagnecEmissions 5
GuidanceandManufacturer’sDeclaraon(IEC60601-1-2)
—ElectromagnecEmissions 5
GuidanceandManufacturer’sDeclaraon
—ElectromagnecImmunity 6
Safety/InformaonSymbols 8
Safety and Reliability 9
Temperature Safety 9
Mains Power Supply 10
RFID Reader Environment 10
Startup / Shutdown Procedure 10
 
Data Capture 12
Imaging 12
Traceability 13
Cryo 13
RI Witness™ Work Area 13
RI Witness™ Manager for Traceability, Data Capture and Imaging 14
RFID Overview 15
The Shared RI Witness™ Database 15
Hardware Overview 16
Work Area Reader Types 16
Mulplexer 17
Control Units 17
Unheated Control Unit 17
Heated Control Unit 18
Auto Tuning Readers 18
RFCalibraon 19
RIWitness™WorkAreaSpecicaonTable 20
 
Introducon 21
RI Witness™ RFID tags 22
TaggingPlascware 22
Dishes and Pots 22
Tubes 22
Handling RFID Tags 23
How to Switch On 24
SystemInformaon 24
ToOpentheSystemInformaon 25
How to Shut Down a Work Area 25
Logging In 25
Auto Logon 25
Logging Out 26
The WorkArea Interface 27
Performing A Procedure in RI Witness™ 28
Beginning a New Cycle in RI Witness™ 28
WitnessPointConrmaon 30
Double Witness Points 30
QuesonWitnessPoints 31
Record Witness Points 32
Unassigned Tags 32
Tag Mismatch 33
Unassigned Tag Removal 33
RecordLocaonofTags 34
Training Mode 35
ClearDemoTables(legacyworkareasforpresowareversion2.3only) 35
Admin Assign 36
Discards 36
Cycle Summary 37
CONTENTS
Daily Lists 37
Windowed Work Area 37
Language and Keyboard Layout 38
Foot Pedal Control 38
To Rename the Work Area 39
ToChangetheLocaonofaWorkArea 39
ToViewWorkAreaLocaonsorDisableaWorkArea 40
AlarmifTagsLeinWorkArea 40
 
Logging In 42
License Management 42
License Request Form 43
Database Management 45
InialScreen-MainMenuBuons 46
Home 46
HowtoConguretheHomeScreen 47
Paents 48
EnteringNewPaentDetailsintoRIWitness™Manager 48
EdingExisngPaentDetails 49
LookingUpPaentHistories 49
Assigning Donor Status 50
Assigning Partners 50
Cycle Types 51
StarngaNewCycle 51
Assign a Cycle Type 51
ColleconandTransferDates 52
Cycle Status 52
Revert Status 52
RIPaentIdentyLabels 53
PrinngPaentLabels 54
Label Styles 55
Operator Management 55
Operator Details 56
Group Membership 56
Enabled Operators 56
Operator PIN 56
CONTENTS
Clinic Details 56
Mismatch Comments In RI Witness™ Manager 57
The Witness Point Diagram 58
Links 59
Reassigned Tags 59
Unassigned Tags 59
Witness Point Inputs 60
MulpleSourcesoftheSameTagType 60
MulpleTagTypes 60
Entry Witness Points 60
Double Witness Points 60
Donor Witness Points 61
TaggedDonaons 61
UntaggedDonaons 62
WitnessPointQuesons 62
Witness Point Order 63
The Witness Point Log 63
Tag Types 63
ExploretheWitnessPointLog 64
WitnessPointLogStascs 64
 
AnIntroducontoCryoWitnessing 65
PrinngBarcodes 65
Scanning Barcodes 67
 
Managing Materials 68
Material Types 68
Material Type Details 69
Notes on GS1 Barcodes 70
Cycle Types 70
CreangaNewBatch 70
DelengaBatch 72
Batch Status 72
ExpiredBatches 73
View All Batches 74
CONTENTS
Paents-ManagingTreatmentCycles 74
ExcludingandIncludingBatchesforaCycle 74
 
AnIntroducontoDataCaptureforDataCollecon 75
Collected Data Setup 75
ParameterConguraon 76
Numerical Parameters 76
TextParameters 76
List Parameters 77
Check Parameters 77
Date, Time and Date Time Parameters 78
CalculaonParameters 78
Built In Parameters 78
Sheet Contents 78
Shared Between Sheets 79
Treatment Cycle Sheets 80
ViewingandEdingSheets 80
EdingSheetsattheWorkAreaTouchScreen 80
ViewingandEdingSheetswithRIWitness™Manager 82
Egg Parameters 82
The Number of Eggs 84
Egg Count Parameter 84
EggCountConict 84
Sheets and Witness Points 86
Cycle Summary Sheets 86
ParameterAributes 87
InialValue 87
Editable 88
Required 88
 
How to View Live Images 89
MulpleScreenConguraon 89
How to Take a Picture 90
How to Record Video 91
How to Zoom & Pan the Image 91
CONTENTS
How to Perform Measurements 91
How to Select Cameras 92
Camera Flipping 92
How to Set the Preset Zoom 92
HowtoConguretheFootPedal/Keyboard 92
HowtoSelectObjecves 92
HowtoAddObjecves 93
HowtoRemoveObjecves 93
HowtoCheckObjecveCalibraon 93
PaentDisplay 93
Use of Saturn™ Laser Systems with RI Witness™ 94
HowtoAccessSaturnLaserSystemInformaonfromaWorkArea 94
 
 
TemperatureProle 97
TemperatureProleChecklist 97
TemperatureCalibraon 97
TemperatureCalibraonService 98
TouchScreenCalibraonChecklist 99
 
Cleaning 100
 
Reuse Statement 101
RI Repairs System 101
Product Disposal (European Union) 101
RI Returns System 101
Contact Details 101
ObligaontoInform 101
Feedback 102
CONTENTS
Secon 1
RI Witness™ RFID Tags
1
1


Thank you for choosing RI Witness™.
The RI Witness™ family of products is made up of RI Witness™, Data Capture, Traceability, Imaging and
Cryo.
ThismanualprovidesallnecessaryinformaontouseRIWitness™.Thesystemshouldbeoperated
bytrainedpersonnelonly.Allseconsofthismanualshouldbereadandunderstoodfullybeforeany
operaonofthesystem.PleaseseetheIntendedUseformoreinformaon.
IftheoperatorisunsureofanyoftheinformaoncontainedinthismanualtheyshouldcontactResearch
Instrumentsoranappointedrepresentavebeforeaempngtousethisequipment.
In no event does Research Instruments Ltd (RI) assume the liability for any technical or editorial errors
ofcommission,oromission;norisRIliablefordirect,indirect,incidental,orconsequenaldamages
arising out of the use or inability to use this manual.
The informaon in this manual is current at the me of publicaon. Our commitment to
product improvement requires that we reserve the right to change equipment, procedures
and specicaons at any me. The latest version of the User Manual can be downloaded from
soware.research-instruments.com.TheRI Witness™ manual belongs with the RI Witness™system
and should be passed on with the system if relocated to another clinic.
The use of ™ in this manual indicates a trademark of Research Instruments Ltd. Any other brand
names,referredtointhismanual,aretrademarksoftheirrespecveowners.
© This manual is protected by copyright, all rights reserved, and no part here of may be photocopied
orreproducedinanyformwithoutthepriorwrienconsentofRI.
             


Secon 2
2
Introducon
2


For RI Witness™ heated work areas only.
Toidenfyandtrackhumansamplesthroughtheassistedreproducon(AR)cycleandwhererequired,
to maintain sample temperature.
Therearenocontraindicaonsassociatedwiththeuseofthisdevice.

Theheatedplate(andtemperaturecontrol)componentsofthissystemareclassiedasaclass
IImedicaldeviceinaccordancewithArcle9,AnnexIX,rule9oftheDirecves93/42/EEC&
2007/47/EC,ieit‘administersorexchangesenergy’toorfromthehumanbody.Inrespect
oftheRIWitness™system,energyisemiedintheformofheat,toorfromtheARsample.
Thereisnodirectpaentcontact.
Applicable indicaons for use are subject to the regulaons of the country into which the device is sold.
Availability of RI Witness™ for clinical use is dependent on the regulatory approval status of RI Witness™
within the country the device is intended to be sold into.
 
6-70-801 Sit on Top Heated Work Area Assembly
6-70-801/C Sit on Top Heated Work Area Assembly with Card Reader
6-70-801/T Sit on Top Heated Work Area Assembly with Test Tube Reader
6-70-801/TC Sit on Top Heated Work Area Assembly with Card and Test Tube Reader
6-70-802 FlushFiedHeatedWorkArea1ChannelAssembly
6-70-802/C FlushFiedHeatedWorkArea1ChannelAssemblywithCardReader
6-70-802/T FlushFiedHeatedWorkArea1ChannelAssemblywithTestTubeReader
6-70-802/TC FlushFiedHeatedWorkArea1ChannelAssemblywithCardandTestTubeReader
6-70-803 Slim Heated Work Area Assembly
6-70-803/C Slim Heated Work Area Assembly with Card Reader
6-70-803/T Slim Heated Work Area Assembly with Test Tube Reader
6-70-803/TC Slim Heated Work Area Assembly with Card and Test Tube Reader
6-70-804 FlushFiedHeatedWorkArea2ChannelAssembly
6-70-804/C FlushFiedHeatedWorkArea2ChannelAssemblywithCardReader
6-70-804/T FlushFiedHeatedWorkArea2ChannelAssemblywithTestTubeReader
6-70-804/TC FlushFiedHeatedWorkArea2ChannelAssemblywithCardandTestTubeReader

Secon 2
Introducon
3

2

RIWitness™isusedinconjunconwiththefollowing:
• Essenalmedicaldevices-dishesandtubes,maybeARornot-ARspecic.
• Nonessenalmedicaldevices-safetycabinets,incubators,micromanipulators,lasers.
• Non medical devices (general laboratory equipment), eg work benches, microscopes, PCs.

InstallaonsofRIWitness™shouldbecarriedoutbyaRItechnicianorotherRIauthorisedpersonnel.
Incorrectinstallaoncouldresultinoverallpoorperformance.
 
6-70-805 ITO Reader
6-70-805/C ITO Reader with Card Reader
6-70-805/T ITO Reader with Test Tube Reader
6-70-805/TC ITO Reader with Card Reader and Test Tube Reader
6-70-806 Sit on Top Heated Work Area 2 Channel Assembly
6-70-806/C Sit on Top Heated Work Area 2 Channel Assembly with Card Reader
6-70-806/T Sit on Top Heated Work Area 2 Channel Assembly with Test Tube Reader
6-70-806/TC Sit on Top Heated Work Area 2 Channel Assembly with Card and Test Tube Reader
6-70-852 SpermPreparaonWorkAreaAssembly
6-70-852/C SpermPreparaonWorkAreaAssemblywithCardReader
6-70-853 Unheated Work Area Assembly
6-70-853/C Unheated Work Area Assembly with Card Reader
6-70-853/T Unheated Work Area Assembly with Test Tube Reader
6-70-853/TC Unheated Work Area Assembly with Card and Test Tube Reader
Secon 3
Safety Warnings
3

disassembleormodifyanypartoftheRIWitness™,orsubstuteanycomponent
for any other. Doing so may result in damage to samples. This voids the warranty and/or
service contract.
 To avoid the risk of electric shock, this equipment must only be connected to a
supplymainswithprotecveearth.
 use the power cable and power supply adaptor supplied with the system.
The cable to the power supply is the ‘disconnect device’ for this equipment. To remove all
electrical power from this product, disconnect the power cable from the electrical outlet.
Equipmentshouldbeposionedsoastoalloweasyaccesstothepowercable.Theappliance
coupler or mains plug is used as the disconnect and must remain readily operable.
Nottobeusedinapaentenvironment.
Thesystemshouldbeoperatedbyqualiedandtrainedpersonnelonly.
Thissymbolindicatescauonarytextwhichshouldbefollowedtoavoidinjurytousersor
damage to samples.
Secon 3
Safety Warnings
5

3
RIWitness™isintendedforuseintheelectromagnecenvironmentspeciedbelow.Thecustomeror
the user of RI Witness™ should ensure that it is used in such an environment.


  
RF emissions
CISPR 11 Group 2
RI Witness™ must emit electromagnec energy
inordertoperformitsintendedfuncon.Nearby
electronicequipmentmaybeaected.
RF emissions
CISPR 11 Class B
RI Witness™ is suitable for use in all establishments
otherthandomescandthosedirectlyconnected
to the public low-voltage power supply network
that supplies buildings used for domesc
purposes.
Harmonic emissions
IEC 61000-3-2 Not applicable
Voltageuctuaons/
ickeremissions
IEC 61000-3-3
Not applicable


 This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuanttopart15oftheFCCRules.Theselimitsaredesignedtoprovidereasonableproteconagainst
harmful interference when the equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance
withtheinstruconmanual,maycauseharmfulinterferencetoradiocommunicaons.Operaonof
thisequipmentinaresidenalareaislikelytocauseharmfulinterferenceinwhichcasetheuserwillbe
requiredtocorrecttheinterferenceathisownexpense.
ThisdevicecomplieswithIndustryCanada’slicence-exemptRSSs.Operaon issubject tothe
followingtwocondions:
1. This device may not cause interference.
2. Thisdevicemustacceptanyinterference,includinginterferencethatmaycauseundesiredoperaon
of the device.
Secon 3
6
Safety Warnings
3


 IEC 60601

 

Electrostacdischarge
(ESD)
IEC 61000-4-2
± 6 kV contact
± 8 kV air
± 6 kV contact
± 8 kV air
Floors should be wood,
concreteorceramicle.
Ifoorsarecovered
withsynthecmaterial,
therelavehumidity
should be at least 30 %.
Electrical fast transient/
burst
IEC 61000-4-4
± 2 kV for power supply
lines
± 1 kV for input/output
lines
± 2 kV for power
supply lines
± 1 kV for input/
output Lines
Mains power quality
should be that of a
typical commercial or
hospital environment.
Surge
IEC 61000-4-5
± 1 kV line(s) to line(s)
± 2 kV line(s) to earth
±1kVdierenal
Mode
± 2 kV common mode
Mains power quality
should be that of a
typical commercial or
hospital environment.
Voltage dips, short
interruponsandvoltage
variaonsonpower
supply input lines
IEC 61000-4-11
<5 % UT
(>95 % dip in UT ) for
0.5 cycle
40 % UT
(60 % dip in UT) for 5
cycles
70 % UT
(30 % dip in UT) for 25
cycles
<5 % UT
(>95 % dip in UT)
for 5s
<5 % UT
(>95 % dip in UT) for
0.5 cycle
40 % UT
(60 % dip in UT) for 5
cycles
70 % UT
(30 % dip in UT) for 25
cycles
<5 % UT
(>95 % dip in UT)
for 5s
Mains power quality
should be that of a
typical commercial or
hospital environment. If
the user of RI Witness™
requiresconnued
operaonduringpower
mainsinterrupons,it
is recommended that RI
Witness™ be powered
fromanuninterrupble
power supply or a
baery.
Power frequency
(50/60 Hz)
magneceld
IEC 61000-4-8
3A/m 3A/m Power frequency
magnecelds
should be at levels
characteriscofa
typicallocaonina
typical commercial or
hospital environment.
UTisthea.c.mainsvoltagepriortoapplicaonofthetestlevel.
Secon 3
Safety Warnings
7

3


 IEC 60601





Conducted RF IEC
61000-4-6
Radiated RF IEC
61000-4-3
3 Vrms
150 kHz to 80 MHz
3 V/m
80 MHz to 2.5 GHz
3 Vrms
3 V/m
PortableandmobileRFcommunicaons
equipment should be used no closer to
any part of RI Witness™, including cables,
than the recommended separaon
distance calculated from the equaon
applicable to the frequency of the
transmier.
Recommendedseparaondistance
d = [3.5/V 1]√p
d = [3.5/V1]√p80MHz to 800MHz
d = [3.5/V1√p800MHz to 2.5GHz
where p is the maximum output
power rang of the transmier in
was (W) according to the transmier
manufacturer and d is the recommended
separaon distancein metres(m). Field
strengths from xed RF transmiers,
as determined by an electromagnec
site survey, a should be less than the
compliance level in each frequency
range.b Interference may occur in the
vicinity of equipment marked with the
following symbol:
At 80 MHz and 800 MHz, the higher frequency range applies.
Theseguidelinesmaynotapplyinallsituaons.Electromagnecpropagaonisaectedby
absorponandreeconfromstructures,objectsandpeople.
Theseguidelinesmaynotapplyinallsituaons.Electromagnecpropagaonisaectedby
absorponandreeconfromstructures,objectsandpeople.
aFieldstrengthsfromxedtransmiers,suchasbasestaonsforradio(cellular/cordless)telephones
and land mobile radios, amateur radio, AM and FM radio broadcast and TV broadcast cannot be predicted
theorecallywithaccuracy.ToassesstheelectromagnecenvironmentduetoxedRFtransmiers,
anelectromagnecsitesurveyshouldbeconsidered.Ifthemeasuredeldstrengthinthelocaonin
whichRIWitness™isusedexceedstheapplicableRFcompliancelevelabove,RIWitness™shouldbe
observedtoverifynormaloperaon.Ifabnormalperformanceisobserved,addionalmeasuresmay
benecessary,suchasre-orienngorrelocangRIWitness™.
bOverthefrequencyrange150kHzto80MHz,eldstrengthsshouldbelessthan[V]V/m.
Secon 3
8
Safety Warnings
3

 
Indicatesinstruconfordisposalofgoods.
In accordance with Annex II of the European
MedicalDeviceDirecve93/42/EEC,asamended
byDirecve2007/47/ECunderthesupervisionof
noedbodyNo.0120,SGS,UKLtd.
In accordance with the European Direcve for
R&TTE,Direcve1999/5/EC
Indicates the medical device manufacturer.
Indicates the need for the user to consult the
instrucons for use for important cauonary
informaonsuchaswarningsandprecauonsthat
cannot, for a variety of reasons, be presented on
the medical device itself.
Followinstruconsforuse.
Therstfourdigitsareauniqueidenerassigned
to the product and the last 2 digits signify the year
of manufacture, eg 5001/13 (this denotes a unique
serial number of 5001 and a year of manufacture
of 2013).
Cauon: US Federal law restricts this device for
sale to or on the order of a licensed healthcare
praconer.
S
N
Only
Secon 3
Safety Warnings
9

3

Pleasereadthismanualcarefullyandfollowtheinstruconstoensurethatthesystemwillworksafely
and reliably.

Safetyistheresponsibilityofthelaboratory.Riskassessmentandworkingpraccesshouldcomplywith
local regulatory policies.
A warning triangle will be displayed on the work area touch screen and on the control unit display if the
currently selected temperature cannot be maintained.
A touch screen warning triangle will also be shown if temperature control has been disabled using the
TurnObuon.
Gently place your hand on the heated surface to verify that the temperature is appropriate for use.
Aswithallheangsystems,itisadvisabletoperformaperiodiccheckoftemperaturesusingacalibrated
thermocouple thermometer.
Secon 3
10
Safety Warnings
3


An RI Witness™ system uses readers to monitor a work area. Readers detect RFID tagged containers
that are placed in the work area.
Eachreaderisindividuallytunedtoitsenvironmentandmustnotbereposionedaerinstallaon.The
performanceofRFIDtagdeteconmaybecompromisedbyreposioningreadersandbytheproximity
ofmetalobjectsorelectricalequipmentthatwerenotpresentduringinstallaon.
Forcleaning,readersmaybeliedandreturnedtothesameposion.See“Cleaning”onpage100for
more cleaning details.






RI Witness™ hardware may be damaged by incorrect startup and shutdown procedures.
“Secon5-RIWitness™Basicoperaon”describestherecommendedstartupandshutdownprocedure
for the RI Witness™ work area.

Products from RI require a stable and noise free power supply ( 100V - 240V ). The supply must provide
anearthconnecon.
The RI Witness™ control unit for heated readers contains a mains power supply.
Secon 4
Product Overview
11

4

Welcome to the User Manual for the Research Instruments (RI) RI Witness™ System.
RI Witness™, Data Capture, Traceability, Imaging and Cryo are members of the RI Witness™ family
of products. RI Witness™ products share a common database usually referred to as the “Witness”
database.
RIWitness™isasystemwhichoperateswithinanassistedreproducon(AR)clinicsengandprovides
amethodofidenfyinghumansamplesthroughoutan AR cycle(fromegg and spermcolleconto
embryo transfer). The system is intended to minimise the risks associated with tradional/manual
double-checkingandprovidestheessenalcontrolsnecessarytoensureeggs,spermandembryosare
correctly matched and treated during the AR process.
The RI Witness™ system comprises hardware, rmware and soware components, which can be
congureddependingonthetreatmentacvies,numberofARcyclesconducted,sizeandlayoutof
the AR clinic.
RFID (radio frequency idencaon) technology provides the means of idenfying the containers
(dishes, tubes) in which eggs, sperm and embryos are transferred and stored. The containers are
labelledbyaclinicianwithaspecialRFIDtagwhichhasbeenassignedauniqueidener.Theunique
idenerislinkedtoapaent/couple(specicparentage).
As samples are processed as part of an AR cycle, RFID readers (both heated and non-heated) read the
tagson the containerand their identyand status is conrmedon-screen.If containers containing
samplesofincompableorigincomeintocontactatanystageofthisprocess,thesystemacvatesan
alarm and prompts the clinician to respond.
Figure 4-1 RFID tag communicang with various antennas
An antenna is incorporated into a work area reader. A control unit feeds an RF signal to the antenna. An
RFIDtagispassiveunlenergisedbythesignalfromtheantenna.Theenergisedtagthentransmitsan
idencaoncodebacktothesameantenna.SeeFigure4-1.
AnARprocedureisconductedwithinthemonitoredworkarea.Theprocedureisdenedbyasequence
of Witness Points which are presented on the touchscreen.
RI Witness
Computer Control Unit
Sit on Top Heated
Work Area Reader
RFID Test
Tube Reader
RFID Tag
Mulplexer
Touch Screen
Secon 4
12
Product Overview
4

Hand wrien notes are oen taken whilst performing laboratory procedures. For example embryo
scoresandspermvolumesmightbewriendownonadatasheetandlatermanuallytranscribedinto
aclinicferlitydatabase.
The features of Data Capture allow data sheets to be designed in RI Witness™ Manager and data entry
to be performed in the laboratory using the work area touch screen. See Figure 4-2.
Data entry may also be performed in RI Witness™ Manager.
Figure 4-2
Lower: Data entry using a RI Witness™ WorkArea touchscreen
Upper: Data sheet design using RI Witness™ Manager

With the Imaging feature, you are able to capture images and videos from every microscope in the
laboratory,ateverystageofthepaentcycle,inrealme.Allthelatestinformaononacyclecanbe
accessed immediately from any networked PC. Images can also be sent to the embryo room to show
thepaentpriortoembryotransfer.
TheImagingfeatureiscompablewithRIIntegra™andSaturn™lasers.
Figure 4-3 Example of paent view shown in embryo room
Secon 4
Product Overview
13

4

Traceabilityisasowareproductthatlinkspaenttreatmentcycleswiththebatchesofmaterialsthat
are in use.
A barcode reader may be used to scan batches as they are delivered or made ready for use.
Thelinksbetweentreatmentcyclesandmaterialbatchesmaybeexploredtogeneratevariousreports,
forexampleareportshowingallpaentsthathavebeenexposedtoaparcularmaterialbatch.

TheCryofeatureextendstheRIWitness™securitysystem,allowingpaentsamplestobetrackedas
theyenterandleavecryostorage,creangacompleterecordofthepaentcycle.
A barcode reader is placed at the work area and used to scan a sample in or out of the cryo storage.

Figure 4-4 An RI Witness™ work area
RFID tagged items are displayed on a touchscreen running RI Witness™ WorkArea soware.
A typical RI Witness™ work area includes a PC, touchscreen, one or more tag readers and an RF control
unit.TheworkareaPCrunsRIWitness™WorkAreasowaretopresentanoperatorinterfaceonthe
touch screen. See Figure 4-4.
The data presented on the touchscreen is generated from the shared Witness database, all work area
events are logged to the shared Witness database.
Secon 4

Product Overview
4

RI Witness™ Manager runs on any Windows PC and provides Witness, Data Capture, Cryo, Traceability
andImagingfunconality.
The“paents”RIWitness™Managerpageisusedbyalloperators,the“materials”RIWitness™Manager
pageisuniquetoTraceabilityoperatorsandthe“witnesspoints”pageisuniquetoWitnessoperators.
See Figure 4-5.
Figure 4-5 RI Witness™ Manager is used by RI Witness™, Data Capture, Traceability, Imaging and Cryo
Secon 4
Product Overview
15

4

AnexampleRIWitness™installaonisshowninFigure4-6.FourworkareaswithRIWitness™WorkArea
soware, two RI Witness™ Manager PCs and an admin reader PC share a common RI Witness™
database.Traceability, RI Witness™ and DataCapturemanagementfuncons maybe performedat
each RI Witness™ Manager PC.
Lab
Manager
Work Area
Work Area
Shared RI Witness™ Database
Lab
Manager
Admin Reader
Work Area
Work Area
Figure 4-6 The RI Witness™ database is shared by All RI Witness™ WorkArea and RI Witness™ Manager PCs

Radio-frequency idencaon (RFID) is the technology used by RI Witness™ to idenfy tagged
plascware.
AcontrolunitfeedsanRFsignaltoamatchedantenna.AnRFIDtagispassiveunlenergisedbythe
signalfromtheantenna.The energisedtagthentransmitsanidencaon codebacktothe same
antenna. See Figure 4-1.
AnRFIDsystemworksontheprincipleofinducvecoupling,whichrequiresthattheantennabetuned
tomatchthefrequencyinuseandopmisedforthephysicalenvironment.Speciccablelengthsmust
beusedforallRFconnecons.
EachRIWitness™workarearequiresacomputer,acontrolunitandatleastoneantenna.Mulple
antennaworkareasrequireamulplexertoswitchtheRFsignalbetweenantennas.
Antennas are built into RI Witness™ Readers.
Secon 4
16
Product Overview
4


ReadersdetectRFID(RadioFrequencyIdencaon)tagsusingtunedantennas.Thetesttubereader
(2axis)andthespermpreparaonreader(3axis)useonetunedantennaforeachaxis.Allotherreaders
use a single tuned antenna.
Aushngvariantoftheheatedreaderisavailable,thisisintegratedintothesurfaceofthesafety
cabinetforeaseofuse.Legscanbeedtoallowfurtherintegraonwithotherlightbases.
Figure 4-7 ITO reader Figure 4-8 Card reader Figure 4-9 Admin reader
Figure 4-10 Sit on top heated reader Figure 4-11 Sit on top unheated reader
Figure 4-12 RFID Test tube reader Figure 4-13 Sperm preparaon reader
Secon 4
Product Overview
17

4

AmulplexerdistributesRFpowertomulpleantennas.Amulplexercanfeeduptofourantennas.

Control units provide the RF power for readers. They may be used for heated or non-heated work areas
depending on the procedures conducted there.
Figure 4-14 An RI mulplexer

Theunheatedcontrolunitmaybeusedwithamulplexertodriveuptofourantennas.

 Mains socket
 USB to PC
 RFCoaxialOUTtomulplexerorheatedreader
Figure 4-15 Back panel of unheated control unit
1 32
Secon 4
18
Product Overview
4

Theheatedcontrolunitcontainsatemperaturecontrollerandmaybeusedwithamulplexertodrive
up to four antennas.
1 2 3 4

1. RFCoaxialOUTtomulplexerorheatedreader
2. USB to PC
3. Heater cable to reader
4. Mains socket
Figure 4-16 Back panel of heated control unit


1. Place the reader in the desired area
2. Remove the cable clamp
3. Connect the power supply
4. Connect the Mini-USB connector
5. Replacetheretainingbracketandghtenscrew
6. Connect the USB-A connector to the PC, Tablet or USB
ConneconsaremadeupofaUSBA-Bcableandapowersupply.Thepowersupplyisa12Vpower
supplyandisexternaltothedevice.TheUSBisastandardUSB2.0cable.Thesearelocatedontherear
of the device and held in place by a retaining bracket and screw.
Figure 4-17 Sperm preparaon reader
Secon 4
Product Overview
19

4

CalibraonoftheRIWitness™SpermPrepReaderismadealotsimplerbyhavinganautomactuning
system. The procedure for this is as follows.
1. OpenRIWitness™WorkArea.MakesuretheRFIDreaderandMulplexeraredetected.
2. Click the buonontheboomrightofthiswindow.
3. FromthisyouwillseetheWorkareaSengswindow.ClickontheRFIDReadingtabtobringup
theRFIDConguraonWindow.
Figure 4-18
Figure 4-19
4. FortheSpermPrep,settheAntennaConguraonto3.Thenextthingtodoistoclickon
.Thiswilltunetheantennasfortheareatheyaresituatedin.Everymethedeviceis
moved this will need to be done. This will take a few moments but when done the SWR for each
channel should show a value of 1-50. If there are any RI tags in the work area, then they will be
displayedinthegreybox.YouarenowreadytousetheRIWitness™SpermPrepReader.
Secon 4
20
Product Overview
4

 
HeangSystems* Heated metal and glass.
TemperatureSensor* Digital 14-bit SHT-15 or Analogue PT1000.
Displays* LCD Display. Accurate to 1 decimal place.
Connecvity USBTypeBsocketforconnecontoPC.ConnectedPCtobecompliant
with IEC 60950-1.
Supply Voltage 100-240VAC,50-60Hz,Max.2.6A,ClassI.
OperangTemperature* Temperature: 10°C (50°F) to 42°C (107.6°F).
Humidity: 15% to 85% RH (Non Condensing).
HeaterChannels* 2Channel122VDCat6AMax.Channel222VDCat1Amax.
RFID 50ΩLoadat13.56Mhz1.4WMax.I-4Antennas.
*Forheatedworkareasonly
Secon 5
RI Witness™ Basic Operaon
21

5


RI Witness™ uses one or more readers to monitor RFID tagged containers in a work area.
The work area interface is presented on a touch screen. See Figure 5-1. The operator touches screen
buonstologinandselectWitnessPoints.
Figure 5-1 The RI Witness™ WorkArea interface is presented on a touch screen
Secon 5
22
RI Witness™ Basic Operaon
5


Beforeuseintheworkarea,allplascwaremustbeRFIDtaggedandlabelledwithpaentidenty.
Rectangular,circularandsquaretagsareavailable.SeeFigure5-2.Thesampleidentymaybeassigned
totheRFIDtagoncethetaggedplascwareisplacedinaRIWitness™workarea.

RFIDtagsmustbeposionedonthebaseofdishesandpots.Thesquaretag,posioneddiagonally,is
recommended for a four well dish.
Figure 5-2 RI Witness™ tags
            




Therectangulartagisrecommendedfortubes.Posionthelongedgeofthetagalongthelengthof
thetubeandholdinplaceusingtapeorapaentidentylabel.Posiontagsnearthetopoftubesto
ensure they are not obscured by thermal blocks or tube warmers.
Rectangular Tag
Square Tag
Circular Tag
Dia 43.8mm Bridge
Chip
Peel from here
36 mm
18 mm
Bridge
Chip
+ UPM
Peel from here
18 mm
18 mm
Bridge
Chip
+ UPM
Peel from here
375_1
+
22 mm
12 mm
Peel from here
Chip
Bridge
Time-Lapse Tag




Secon 5
RI Witness™ Basic Operaon
23

5

When removing tags, bend the backing strip away from the tag, rather than the tag away from the
backing strip. This will reduce the risk of damaging tags.
1. Startpeelingthebackingstripawayatthe“peelfromhere”pointshowninFigure5-3toachieve
a peel line as shown.
chip and bridge line peel line
Figure 5-3
Figure 5-4
 
2. Whensckingthetagtothedish,bringthetagintocontactwiththedishatonepoint.Thenuse
yourngerorthumbtoworkthetagontothedishwithacircularmovement,movingawayfrom
theinialcontactpointsoastoavoidanykinksinthetagasitsckstothedish.SeeFigure5-4.

Unusedtagsandunusedtaggedplascwareshouldnotbestoredonornearasurface(safetycabinet
or workbench) where a RI Witness™ reader is located.
Secon 5

RI Witness™ Basic Operaon
5

RI suggests that you keep the RI Witness™ computers and work areas switched on. This means that the
heangandmonitoringisconstant.
IfyouhaveswitchedoaheatedRIWitness™workarea,pleaseallow45minutesfortheworkareato
return to the required temperature. Please check there is power at the wall socket, the PC and touch
screen (or tablet) and control tower.
You may want to check the work area heang and touch screen calibraon for accuracy before
commencingwork.See“Secon12-TemperatureCalibraon”onpage97.

Thesysteminformaonpanelwillshow:
1. Versionnumbersforsowareandrmware
2. Workarealocaon
3. Database version
4. Licensing status including Imaging dongle
5. RFIDreaderstatusandmulplexer
6. Temperature control status
7. Saturn™LaserSystemstatus,serialnumber,rmwaredetails
8. Camera status
9. Integra™ status
6
1
2
3
5
7
8
9
Figure 5-5
Secon 5
RI Witness™ Basic Operaon
25

5
Figure 5-6 RI Witness™ WorkArea icon

1. Press the  icon in the work area or click the  icon from the Imaging
screen.
2. TheSystemInformaonwindowshowsthecurrentstateoftheRIWitness™system.
3. Indicates there is no problem.
4. Indicates a problem needs resolving.
5. InsystemInformaon,clickingontheclosed attheboomofthepagewillunlock
alltheexisngconguraonsengsandalsorevealthefollowingsengsthatarehiddenwhen
theSystemInformaonislocked:
• Keyboard / foot pedal controls
• Auto logon
• Language
• Screenconguraon
TheSystemInformaonislockedbydefaultandclickingontheagainwillre-lockthesengs.

DonotswitchoanyRIhardwarewhilsttheWorkAreasowareisrunning.Tologout,selecttheuser
name, and then select  in the user name window. The system will then shut down. See Figure 5-8.

ItisimportanttoensurethatallRIWitness™hardwareisswitchedonbeforetheworkareasoware
isacvated.
TheRIWitness™sowaremaybeacvatedautomacallywhenthecomputerisstarted.Ifnot,double
click the  desktop icon.
To log in either touch  then your username or scan your operator ID tag and enter the appropriate
4 digit PIN if requested.
Operators can change the PIN assigned by the administrator and may assign an ID tag. See Figure
5-9.

Aerunlockingthesysteminformaon,screennavigatetotheautologonsecon.Thereyoucansetup
the username and password (of the machine) for RI Witness™ to auto logon the PC.
1. Click 
2. Unlocksengs.
3. Click 
4. Click  to the user  message.
5. Enter the details required (contact IT department if unsure).
6. Click 
7. Tick 
8. Click the close window cross.
Oninstallaonoftheapplicaontheapplicaonshortcutisplacedinthestart-up.Thiscausesthework
areatoautomacallystartwithwindows.
Figure 5-7 AutoLogon
Secon 5
26
RI Witness™ Basic Operaon
5

Logoutoftheworkareawhenyouhavenishedyourproceduresbypressingyour in the
top right corner, then the  .Anautomaclogoutmaybeprogrammedaeraperiodof
inacvity.ThisissetusingtheRIWitness™ManagerSengspage.
Figure 5-9 RI Witness™ WorkArea logon and PIN entry windows
Figure 5-8 Logging into RI Witness™
Secon 5
RI Witness™ Basic Operaon
27

5

The WorkArea interface screen is divided into 7 regions. See Figure 5-10 .

 
Thisregionshowsthecurrentpaentname,dateofbirthandID.Whentagsfrombothpartners
arepresentthenameshownwillbethefemalepaent.Thepartner’snamewillappearin
brackets.
 
Therecenthistoryregionpresentsthefourmostrecentaconsthathavebeenrecordedagainst
thecurrentpaent,egwitnesspoints(acon/protocolstep),operatormismatches,etc.A
mestampandoperatornamearealsoshown.
 
The work area region displays an icon for each RFID tag detected by the work area. As
tags progress through the witness points of a procedure they are assigned and reassigned
appropriately.Aquesonmarkiconwillbeshownforanytagsthathaveyettobeassignedan
identybytheRIWitness™system.
 
The status region summarises the work area contents. It is recommended that the number of
tagsdisplayedinthestatusareaisvisuallyconrmedateachstageofaproceduretoverifythat
each tag has been detected.
 
For heated systems this region displays the temperature of the work area. Click the temperature
readout to show a  window from where the temperature may be set.
Emily
Figure 5-10 The WorkArea interface
1
2
3
5
6
7
Secon 5
28
RI Witness™ Basic Operaon
5
 
Thewitnesspointsregiondisplaysoponsavailableforthenextstepasspeciedbythelab
denedprotocol.Thetoponeistheprecedingaconandthecurrentcontentsoftheworkarea
determinewhatnextstepsarepossible.Anadministratorwillalsoseeanoponto
assign or reassign dishes outside of the prescribed protocol.
 
Thecurrentoperatorregionshowswhoisloggedon.Asux(Admin)isaddedforoperatorswho
belongtotheadministratorgroup.Allwitnesspointsperformedwillbeaributedtotheoperator
whoisloggedinatthatme.

Bringthedishortubeyouwishtoworkonintotheworkarea.Ifthetaghas beenidenedbyRI
Witness™thepaentandthehistorywillbeshownonthescreen.Theoponsforthenextaconswill
be shown in yellow on the witness point region.
Intheexampleshownbelowa“SpermWashTube”andan“EggCultureDish”havebeenplacedinto
the work area and RI Witness™ has determined that the only appropriate procedure is represented by
the  witness point. See Figure 5-11.
Figure 5-11 The IVF inseminaon witness point is the only match for the contents of this work area

TherststepintheRIWitness™procedureiswhentheidentycardforthepaentisassigned.This
isthemostimportantstepintheprotocolasiftheidentyofthepaentiscorrectlyassignedatthis
point, the security of the cycle is assured.
Secon 5
RI Witness™ Basic Operaon
29

5
Figure 5-12 Unassigned tag has been detected
Figure 5-13 Choose Paent/All Paents window to assign ID

1. To start a new procedure, log in then place one or more unassigned, tagged items into the work
area(usuallyapaentIDcard,eggcollecondishorspermpot).Ayellowquesonmarkwill
show that the tag has been detected and is unassigned. See Figure 5-12. Press the icon.
2. In the tabtypethepaentnameorIDnumbertolocatethecorrectpaentinthe
database. See Figure 5-13.
3. Alternavely,thetabshowsallEggColleconsandEmbryoTransfersscheduledfor
theday.ClickthepaentcycleforeitherEggColleconorEmbryoTransfer,thiswilldisplaythe
chosenpaentsdetailsintheworkarea.
4. Choosewhichaconyouintendtoregister/performandthenclickthe icon again.
5. If a double check by another person has been set at this point, a 2nd Witness window will
appear.ThisrequiresacolleaguetoverifytheaconbyinpungtheirnameandPIN.See
“DoubleWitnessPoints”onpage30.
Secon 5
30
RI Witness™ Basic Operaon
5

Ateverystepintheprocedure,awitnesspoint(acon)mustberegisteredinthesystem.Therefore
whenanaconisperformed,selecttherelevantwitnesspointandconrmbycheckingthedetailsin
theconrmaonwindowandclickingthe icon .
Figure 5-14 Conrming a witness point
Figure 5-15 A double witness point

Cricalpoints,asdenedbytheadministrator,mustbewitnessedbyasecondoperator.Thesecond
witness must enter their PIN. See Figure 5-15.
Secon 5
RI Witness™ Basic Operaon
31

5

Queson Witness Points, as dened by the administrator, require a response from the operator.
ExamplesoftextandnumericquesonsareshowninFigure5-16andFigure5-17.
The Witness Point queson and the operator response is displayed during the nal witness point
conrmaonwindow.Thequesonandresponsealsoformpartofthehistoryloggedforthisevent.
Figure 5-16 Add witness point queson
Figure 5-17 Edit witness point queson
Secon 5
32
RI Witness™ Basic Operaon
5

Thetagsonunusedplascwareareunassignedandareshownasaquesonmarkiconintheworkarea
display. SeeFigure5-18.Whenselecngawitnesspointunassignedtagsareautomacallyassignedto
thepaentcurrentlydisplayedonthescreen.Theywillbeidenedasthesampletagdenedbythe
witness point.
Figure 5-18 The queson mark icon represents an unassigned tag

Gatherallthe(tagged)plascwarethatarerequiredtoperformaprocedureandplacetheminthe
work area.
Awindowrepresenngtheworkareawilldisplayallthetagsandshowtheidentyofanytagsalready
“known”tothesystem.
An acve paent is selected either manually or automacally; manually by clicking on the 
seconoftheworkareaandchoosingapaentfromthelist,orbythesystemautomacally
selecngthepaentidenedbythetagsintheworkarea:
• Ifthereisonepaentintheworkareathenthisisthecurrentpaent.
• Ifthereare2paentsintheworkareawhoaremale/femalepartnersthenthefemalepaentis
thecurrentpaent.
• Ifthereare2ormorepaentsaeradonaonwherethepaentsaredonor/recipient(s)then
thedonoristhecurrentpaent.
Alistofpossiblewitnesspointsisshown,determinedbytheselectedpaentandvisibletags.Choose
the appropriate witness point.
Ifthewitnesspointisataggeddonaontype,thentherecipientwillneedtobechosenfromalistat
this point.
Ifthewitnesspointrequiresfurtherinformaonenteredbytheoperator,thenthiswillbeaskedfor.
If the witness point requires a 2nd witness, then another operator will now need to choose their name
and enter their PIN.
Aconrmaonwindowsummarisesallthedetailsrelangtothewitnesspointthatwillberecorded.
Ifallthedetailsarecorrect,thenselecngtheckwillrecordthewitnesspoint.
If any new tags are added to the work area during the processing of a witness point (or admin assign)
then the witness point will be cancelled. Also, if during the processing of a witness point one of the tags
in the work area drops out of range of the reader(s), then the witness point can be completed and the
tag(s) will be reassigned successfully.
Secon 5
RI Witness™ Basic Operaon
33

5Figure 5-19 Unassigned tag removal

A  warning is displayed when an unassigned tag is removed from the work area. See Figure 5-19.
Thismessageinformstheoperatorthatanunassignedtagenteredandexitedtheworkareawithout
being registered in the current procedure. If that is correct selectandconnue.Ifnot,replace
the sample/labware in the work area and perform a witness point.

TheRIWitness™systemwillonlyallowonepaent(withdonor)oracouple’ssamplestobeplacedina
workareaatanyme.Ifanunlinkedsampleisplacedintheworkarea,aconnuousalarmwillsoundand
a mismatch window will appear immediately. The  message is displayed.
Figure 5-20 Tag mismatch
1. Remove the samples in order to stop the alarm and select  to close the window.
2. Amismatchreasonwindowisdisplayed.Theoperatormustenteranexplanaonforthemismatch
beforeworkcanconnue.Theexplanaonwillappearinbothpaents’cyclehistoriesandwillbe
visibleintheRIWitness™Managersoware.
:Ifthemismatchoccurredwhilenooperatorwasloggedin,anexplanaonforthemismatchis
associatedwiththeoperatorexplainingbutnotthemismatchitself.
Secon 5

RI Witness™ Basic Operaon
5

Ateachworkarea,alocaonnamecanbesetthroughtheSystemInformaonwindowthatuniquely
idenesthatworkarea.InRIWitness™Manageralistofalltheconguredlocaonscanbeviewed.
Whenatagisplacedintoaworkarea,thelocaonwillbestoredwiththetagdetails;thenwhenthe
tagsassociatedwithapaentaredisplayed,theirlastknownlocaonwillalsobedisplayed.SeeFigure
5-21.
Thenameofeachworkareacanbecongurediniallybyanyuser.Anadministratorcanmodifyany
locaonname.AlistoflocaonscanbeseeninRIWitness™Managerfromwheretheycanbedisabled
andenabled.Disablingalocaonwillcausetherelatedworkarea’snametoberesettoUnknownsoit
canthenberenamedbyauser.Anydisabledlocaoncanbere-enabled.Thiswillsetitsnamebackto
the previous value.
To name a work area:
1. Whentheworkareaisstartediniallyyouwillberequiredtogivetheworkarealocaona
name.Anyusercaniniallysetthenameofaworkarea.Aredcrossiconwillappearaswellas
the  icon as a warning triangle.
2. Click the toopensysteminformaon.
3. Unlockthesengs.
4. Click .
5. Enteralocaondescripon/name.
6. Click the Icon .
Whenawitnesspointiscompleted,thelocaonatwhichittookplacewillberecorded.
PCs connected to the RI database must have unique computer names, if connected to a domain
this will be set, otherwise please check.
Figure 5-21 Tags tab
Secon 5
RI Witness™ Basic Operaon
35

5

Fortrainingandtesng,anyRIWitness™workareamaybesettoTrainingMode.
1. Log in as an admin operator.
2. Open .
3. Select .
4. Thenckthebox.
In Training Mode, the normal work area screen is replaced with an orange background. See Figure 5-22.
OnloggingoutofTrainingMode,thetrainingworkareaeventswillautomacallybedeleted.
Figure 5-22 Training mode
Figure 5-23 The RI Witness™ Manager general sengs page

Training work area events are stored in a demonstraon database which may be cleared using RI
Witness™ Manager. An admin operator may click  on the  page of
RI Witness™ Manager. Figure 5-23.
Secon 5
36
RI Witness™ Basic Operaon
5

Designated operators may use the buon,seeFigure5-24,toassignanytagtype(sperm
pot, inseminated dish, sperm wash tube, etc) to an unassigned tag at any point.
:AdminAssignshouldonlybeusedunderexceponalcircumstancesandbytrainedpersonnel
only.
WithAdminAssignyoucanalsochangethetypeofdishalreadyassignedtoapaent.
Figure 5-24 Admin assign

When a single tag is placed in the work area the buonisenabled,seeFigure5-25.Pressingit
willdisplayaconrmaonscreenwithagreenckandredcross.The will cancel the
discardandclosetheconrmaonwindow,whilstthegreenck will mark the tag as discarded -
changinghowitisdisplayedintheworkareaandprevennganywitnesspointstakingplacewhilstitis
in the work area.
ThereisalsotheoponfromwithinRIWitness™ManagertoenableDiscardstorequireaWitness.
Onceenabled,awitnesswillbepromptedtologinonthescreenfollowingtheoperatorselecngthe
buon,aerasuccessfullogintheconrmaonwindowwillbedisplayedasusual.
Figure 5-25 Discard
Secon 5
RI Witness™ Basic Operaon
37

5

Thecyclesummaryisdisplayedintheworkareaatallmes;itisadatasheetthathasbeenselectedto
beshownfromtheRIWitness™Manager.Ifapaentisnotselected,orthereisnocyclesummaryfor
aselectedpaent,thecyclesummarydisplaywillsay.Iftheselectedpaent
contains a cycle summary sheet, this sheet will be displayed in the  area.

Onthepaentselectwindow,youcanselectthedailylistwhichshowinglistofpaentsfortoday.The
dateisdisplayedinthetopleofthewindow.Tochooseanotherdateandviewitsrelatedprocedures,
clickontheleandrightarrowsatthetopofthewindow.(Thelearrowmovesthedatebackoneday
andtherightarrowadvancesthedatebyoneday).Toselectapaent,clickonthepaentIDnumber.

Foradminstaonswithadminreaders,theWorkAreaapplicaoncanbewindowedtoalloweasyaccess
tothetagreadingfunconality.
1. OpentheRIWitness™WorkAreaapplicaon.
2. Click 
3. Unlockthesengs.
4. Click  / .
5. Tick .
6. Click  and .
7. Ifyouhavemulplescreenssetup,theworkareawillnowreverttoonesingleworkarea
screen.
Figure 5-26 Conrm discard
Figure 5-27 Discard in the work area
Secon 5
38
RI Witness™ Basic Operaon
5

Aerunlockingthesysteminformaonscreen,navigatetotheLanguageandKeyboardsengs.From
there the language on-screen and keyboard style can be changed using a drop-down menu. If the
selected item in either drop down menu is changed, then clicking the  will save these changes
and modify the language and keyboard.
1. Click 
2. Unlockthesengs.
3. Select your required language from the drop down list.

Inthe workareatheSystem Informaon Panelcontainsa  buon
which launches the  dialog.
Withinthekeyboardshortcutconguraondialog,eachavailablecommandislistedfollowedbyan
inputboxandclearbuon(withacross).
Toassignakeyboardshortcuttoacommand,clickinthecorrespondingtext/inputboxandthenhold
down the keys you want to assign to this command. The input boxwill change from “PleaseEnter
ShortcutKey”toalistofthecurrentlyhelddownkeyboardkeysseparatedby+s.Oncethekeyboard
keysarereleasedthosekeysaresavedtotheinputbox.
Pressing the buonwillremovethekeyboardshortcutforthatcommand.
In order to save these changes you must press the buonattheboomofthedialog.
Intheworkarea,oncetheshortcutsaresetup,simplypresstheshortcutkeystoacvatethecommand.
Figure 5-29 RI Witness™ Manager Language Seng
Figure 5-28 Language selecon
4. Select your required keyboard style from the dropdown list.
5. Click the  to save and close the window.
The language used can also be changed from RI Witness™ Manager
1. Click 
2. ChooseyourrequiredlanguagefromthedropdownlistintheLanguagesecon
Secon 5
RI Witness™ Basic Operaon
39

5
List of commands:
• Fire / Enter Laser Mode.
◦Enters laser control mode.
◦ Ifalreadyinlasercontrolmodethiscommandresthelaser.
• Preset Laser Down.
• Preset Laser Up.
• Start / Stop Recording.
• Take Picture.
• Zoom In / Zoom Out.
◦Zooms out fully if already zoomed in.
◦If zoomed out zooms into pre-set zoom value at centre.
• Zoom In.
◦Zooms into centre one step.
• Zoom Out.
◦Zooms out from centre one step.
• Freeze Frame.
  Shortcut keys should be limited to the funcon keys to avoid conicts with typing or
barcodes.

1. Log in as an Admin user.
2. Click 
3. Click 
4. Type the name of the work area using the on screen keyboard.
5. Click the Icon.
Figure 5-30
Figure 5-31

1. Log in as an Admin user.
2. Click .
3. Click .
4. Click 
5. Choosethelocaonrequiredfromthelistorclicktosetanotherlocaonname.
6. Click the Icon to accept the change.
Secon 5

RI Witness™ Basic Operaon
5

1. Open RI Witness™ Manager.
2. Click .
3. Click ,youwillnowseeallnamedworkarealocaons.
4. Highlight the work area and click .
5. Disabledaccountscanbeviewedbycking

Tohelpavoidmismatchesbeingrecorded–“CardLeinReader”.
1. Open RI Witness™ manager.
2. Click .
3. Click .
4. Underworkareasengsck
Figure 5-32
Figure 5-33
Secon 5
RI Witness™ Basic Operaon


5
Figure 5-34
Secon 6

RI Witness™ Manager
6

RI Witness™ Manager can be used on PCs or tablets outside of the RI Witness™ work areas, predominately
foradministraonpurposes.

1. Double click the  desktop icon.
2. Choose your assigned operator name from the list.
3. Enter your 4 digit PIN.
4. Click 
Figure 6-1 Logging in
Figure 6-2 License warning

RI Witness™, Traceability, Data Capture, Cryo and Imaging must be licensed.
LicensinginformaonisstoredinthesharedRIWitness™database.

On each subsequent use of RI Witness™ Manager, a license check is made and a similar license warning
windowwillinformtheoperatorifanylicensesareclosetoexpiraon.
Atanyme,theRILicenseManagermayalsobeinvokedasaseparateapplicaonbyclicking
and then RI License Manager.
Secon 6
RI Witness™ Manager


6
Figure 6-3 RI License Manager

Ifyourlicenseisclosetoexpiraon,youcanquicklyrenewyourlicense.
1. Click in the RI Witness™ Manager license warning window. A license request
form will open. See Figure 6-4.
2. Complete the details on the form.
3. IfyouhaveaRegistraonCodeissuedbyRI,enteritattheboomoftheform.
4. Click aercomplengalltheelds.
5. If internet access is available, a response will be returned by the RI Licensing Server. See Figure
6-5.
Figure 6-4 The license request form
Secon 6

RI Witness™ Manager
6
6. Ifinternetaccessisnotavailable,pleasefollowthedisplayedinstruconstosavealicense
requestle.TheleshouldthenbeemailedtoRIatacvate@research-instruments.com.
7. OnceRIhasreceivedyourrequest,aLicenseDatalewillbeemailedbyreturn.
Figure 6-5 A response from the RI license server
Figure 6-6 The license request email
8. TheLicenseDataleshouldbemadeavailabletotheRIWitness™ManagerPCwherelicensing
is performed.
9. EachmeRIWitness™Managerisopenedfollowinglicenserequest,LicenseManagerwindow
will be displayed. See Figure 6-7.
10. OnreceiptoftheLicenseDatale,clickin the license manager
dialoguebox.Browsetotheemailedlicensedataleandselect.TheLicenseManagerwillnow
be updated with the new license details.
Secon 6
RI Witness™ Manager


6
Figure 6-7 The License Manager window

RI Witness™ Manager must connect to the shared RI Witness™ database.
1. AtRIWitness™Managerlogin,adatabaseconneconswindowwillbeshownifaconnecon
cannot be made. See Figure 6-8.
Figure 6-8 The RI Witness™ database connecon is unavailable
Secon 6

RI Witness™ Manager
6
2. Click the pagebuontoshowthedatabaseconneconsengswindowand
entertheappropriateservername,databasenameandauthencaondetails.SeeFigure6-9.
See the IT Requirements Manual for more details on database management.

Aerlogin,aninialscreenshowsanumberofmainmenubuons.Themainmenubuonspresented
will depend on operator group membership (administrator, normal) and the mixture of installed
products (RI Witness™, Traceability, Data Capture, Imaging and Cryo).

When rst logging into RI Witness™ Manager the Cycles Overview Board is displayed. Clicking the
buononthelehandtoolbarwillreturntheusertotheHomescreen.Thebuonon
theHomescreencausesthedisplaytorefreshbyre-queryingthedatabasetondtheacvetreatment
cycles.
1. Toseethecycledetailsofapaent,doubleclickontherowwiththepaent’sdetails.A
 page will open.
Figure 6-9 Database connecon sengs
Figure 6-10 Home screen
Secon 6
RI Witness™ Manager


6

Eachdayofacycleoraneventcanbegivenadierentcolourcodetoaideasyandquickidencaon
of the cycle status. This can also assist the ordering of procedures.
TocongurethecolourcodingwithintheHomescreen,followthestepsbelow.ExactspellingsofCycle
Types and Events used in the Witness Point Diagram must be used.
1. Click 
2. Click 
3. Firstlyyouwillneedtosetup“States”.Thesecanbedaysoreventsandarecolourcoded.
YoucouldsimplydoDay1,2,3,4,5tostartwith.
4. Click +withintheStateseld.
5. Type the name of the State, eg Embryo Freeze, Day 0, Day 1, etc.
6. Onclickingthebackgroundorforeground(TextColour)box,acolourpickerwillappear.Select
the desired colours.
7. Press 
8. Nextaddacycletype.Clickthe + buonwithintheCycletypeseld.ACycleTypeboxwill
appear. Type in the cycle type carefully, ensuring there are no spelling mistakes. The cycle type
willneedtomatchwhatyouhavealreadysetwithintheTreatmentCycleTypeseldswithinthe
database(See“CycleTypes”onpage70.)AnexampleofacycletypecouldbeIVF,ICSI/IVF,
FET, ICSI, etc.
Figure 6-11 Home screen
9. Click .
10. Select the  drop down arrow you would like to colour code.
11. Click or
12. Assign a day number, ie 3 for Day 3.
13. WithintheMessageeld,typetheDaydescripon,ieDay3.
14. IntheStatedropdowneld,choseoneoftheStatesthathasbeensetup.
15. 
16. Repeat for each Cycle Type.
Secon 6

RI Witness™ Manager
6

Click the buontoshowthewindowifnopaenthasbeenselectedortoview
detailsofaselectedpaent.
1. TypetherstfewcharactersofanameorpaentIDtobrowseaseleconofmatchingpaents.
2. Highlighttherequiredpaentandclickordoubleclicktherequiredpaenttoviewtheir
details.
3. Treatmentcycles,witnesspointsandtagsfortheselectedpaentcanalsobeviewedby
selecngthetabsinthisscreen.
4. ClinicswithCryoenabled,canprintbarcodesfortheselectedpaentbyclicking
. Barcodes printed from here will be recognised by the RI Witness™ system as belonging
totheselectedpaent.
5. Click tocreateadhesivesheetswiththeselectedpaentdetails.Thesecanbe
aachedtoplascwareforthispaent’ssamples.Forfurtherdetailsregardinglabelprinngsee
“PrinngPaentLabels”onpage54.

1. Click thebuontoshowthewindow.
2. Click on buon.
3. Enterdetailsintoappropriateelds–alleldswhichhavebeeneditedarehighlightedinpink.
4. Save all changes.
FromthePaentspagethereisalsoanopontoaddorchooseanewpaent.
 A paent cannot be assigned to a partner unl the partner has also been entered into the
RIWitness™Managersowareseparately–See“AssigningPartners”onpage50.
 Label sheets and barcodes can be printed from this page.
Figure 6-12 Choose paent screen
Secon 6
RI Witness™ Manager


6
Figure 6-13 New paent details

OpenPaentdetailsasoutlinedabove.
1. Click the buon to change to the editable window.
2. Click the buontosavechanges.

1. FromthePaentspage,selectthe tab to view all previous cycles undertaken
forapaentorcouple.
2. Select the  tab to view all witness points registered in an open cycle.
3. Select the tabtoseealllocaonsthattagsassignedtothepaenthavebeenseenbyRI
Witness™.
RefertoFrequentlyAskedQuesonsifnecessary.
Figure 6-14 Looking up paent histories
Secon 6
50
RI Witness™ Manager
6
Figure 6-16 Assign Partners

1. Createanewpaententry,orchooseexisngpaent,leavingthepartnereldblank.
2. Createanewpaentforpartner.
3. Tolinkthe2paents,selectthebuontoshowtheChoosePaentwindow.
4. LocatepartnerbytypingtherstfewcharactersofanameorpaentIDinthesearchwindowto
browseaseleconofmatchingpaents.
5. Clickonthepaentnameandthenconrmseleconbyclicking. See Figure 6-16.

InthePaentdetailswindowselectrelevantdonoropons.
Can Receive Untagged Donations
Can Receive Tagged Donations
Can Donate
Figure 6-15 Assign Donor Status
Theavailability of the donatecheckboxes will dependonthegenderof the paent,ie only female
paentscanreceivedonaons.
Secon 6
RI Witness™ Manager
51

6

Acyclerelatestoaspeciccourseoftreatment(IVF,ICSI,IUI,etc).
Only“administrator”operatorsmaycreatecycletypes.
1. Click the mainmenubuonandthenclick to view all treatment cycle
types. See Figure 6-17.
2. Click  to add each cycle type.
3. Use a right click to  a cycle type.
Traceability operators may specify the range of materials that are used in a cycle type. Data Capture
operators may specify the data sheets that are used in a cycle type.
Figure 6-17 The Treatment Cycle Types Window

1. Click the mainmenubuonandselectapaentbyenteringanameorpaentID.
2. Click the tabtoshowthelistoftreatmentcyclesforthispaent.Fora
newpaentthislistwillbeempty.
3. On the tabofthepaentscreenclick to create a new
cycle.Thenewcycleisshownwithastatusof“InProgress”andacycletypeof“Unknown”.See
Figure 6-18.
4. Traceability operators may click the  tab to view a list of material batches related to
this cycle.
5. All operators may click the  tab to view a list of witness points performed during
this cycle.

Select a cycle type from the pulldown list.
Secon 6
52
RI Witness™ Manager
6
Figure 6-18 Starng a new cycle.
1. Click fromthePaentswindow.
2. Select a Cycle Type from the pulldown on the window.

IfEggCollecon/EmbryoTransferdatesareenteredonthe tab of a treatment cycle then these
events will be available from the work area touchbuon.
See“PerformingAProcedureinRIWitness™”onpage28formoredetailsofpaentseleconusing
.

Anewlycreatedcyclewillbeassignedthestatus“InProgress”.Whenthepaenttreatmenthasbeen
completed click tochangethestatusto“Completed”.

An administrator operator may click the  buonofa“Completed”cycletoreturn
thestatusto“InProgress”.
Secon 6
RI Witness™ Manager
53

6

LaserprintableadhesivepaentidentylabelsareavailablefromRI.
Thesheetprovidesarangeoflabelsizeswithaseleconofnormalandreverseprinng.SeeFigure
6-19.
Figure 6-19 RI laser printable label sheet.
Reverse printed labels are viewable through the base of containers.
Label categories such as “Petri Dish” and “Test Tube” are for guidance only. Please select a label
appropriatetoyourplascware.
“Testtube”labelsmaybeusedtoholdarectangularRFIDtaginposionalongthelengthofatube.
Thereverseprinted“4welldish”labelandthesquareRFIDtagaretherecommendedchoicefora4
well dish. See Figure 6-20.
RILabelsareanoponalcomponentofRIWitness™.
Figure 6-20 Posioning of paent identy labels
Secon 6

RI Witness™ Manager
6

1. FromthepaentdetailswindowclickSee Figure 6-21.
2. Load a blank label sheet into a laser printer. For repeatability of alignment use a manual feed
path and take care when placing each sheet.
3. Setall“SheetAlignment”valuestozeroandclick.
4. Checktheprintedsheet.Measuretheosetsrequiredtocorrectlyposiontheprinteddetails
withinalabelboundary.Payparcularaenontovercalposioningwithinthesmallestlabel.
See Figure 6-22.
Figure 6-21 The label prinng manager
TheexampleshowninFigure6-22showsdetailsofanalignmentcorreconwithintheboundaryofthe
smallest label.
5. Therstprintshowsincorrectalignment.
6. VercalalignmentiscorrectedbyanegaveYoset.
7. HorizontalalignmentiscorrectedbyaposiveXoset.
Secon 6
RI Witness™ Manager
55

6
Figure 6-22 Label text alignment

UsetheLabelLayoutbuonstoselectanappropriatestyle.
Style1isopmisedforpopularplascware,Style2maximisestheuseofavailablespaceandStyle3is
amixtureofStyles1and2.
Arotaonalignmentcorreconisrarelyrequired.
 Check  and select a row number to print a single row of the smallest labels
only. This may be useful if reusing a sheet during the alignment process.

Click  to display a list of all enabled operators. See Figure 6-23.
Figure 6-23 Operator management
Secon 6
56
RI Witness™ Manager
6

1. Click to specify details for a new operator. See Figure 6-24.
2. Select an operator and click to change operator details.
An operator must have a Username, Full Name and Anonymous ID. The Anonymous ID is used for
printed reports.

Groupmembershipisspeciedas“normal”or“administrator”.
Some RI Witness™ features are only available to an “administrator” operator. Administrator only
features include the use of admin assign and making changes to the witness point diagram.
Figure 6-24 Operator Details

Only enabled operators may log in. Click  to remove an operator from the enabled list. Check
“ShowDisabledAccounts”toviewalloperators.

The operator PIN must be a sequence of 4 numbers.

Click the mainmenubuonthenclick to specify the Name of Clinic that will
appear in all printed reports. SeeFigure 6-25.
Secon 6
RI Witness™ Manager
57

6
Figure 6-25 The heading of a printed report will show “My Clinic Name” as specied by “Name of Clinic” on the
general sengs page.

Userscanaddextracommentstofurtherexplainanymismatchesinthehistory
1. Open RI Witness™ Manager.
2. Click the buon.
3. From double click the cycle required to view. This can be seen by clicking
paentsalso.
4. Click the  tab.
5. Double click the  witness point.
6. Click the  icon.
7. Enteraddionalcomments.
8. Click 
Figure 6-26 Report sengs
Secon 6
58
RI Witness™ Manager
6

ThewitnesspointsthatarepresentedtoallworkareaoperatorsaredenedinRIWitness™Manager
by a witness point diagram.
To view the witness point diagram click the mainmenubuonandthenclick. See
Figure 6-27 and Figure 6-28.
Figure 6-27 The sengs screen
Click “Witnessing” to view the witness point diagram
To add a new witness point, click  and then click anywhere in the diagram. Enter a
nameforthewitnesspoint,eg“IVFInseminaon”
UsetheWitnessPointInfopaneltospecifytheaributesofawitnesspoint.Witnesspointaributes,
egreassignan“eggculturedish”tagtobecomean“inseminateddish”tag,aredescribedbelow.
Tagnamessuchas“inseminateddish”mustbedenedbeforetheymaybereferencedbyawitness
point.See“TagTypes”onpage63.
Figure 6-28 The witness point diagram.
Secon 6
RI Witness™ Manager
59

6
Figure 6-29 Detailed informaon for the “IVF Inseminaon” witness point

Thelinks(arrows)betweenwitnesspointboxesrepresenttags(dishes,testtubes,etc)thatarethe
requiredinputsandproducedoutputsoftheproceduredenedbythatwitnesspoint.
To create a link, click  and then drag between two witness points.

Awitnesspointmayrepresentthereassignment(transformaon)ofonetagtypeintoanother,egan
“EggCultureDish”becomesan“InseminatedDish”asthespermisintroduced.
A reassignment is illustrated in Figure 6-29 where the checkboxischeckedtospecify
thattheselectedtagtype“EggCultureDish”willbereassignedasan“InseminatedDish”whenthis
witnesspointisexercisedbytheworkareaoperator.

Witness points may require that unassigned (empty, unused) tags be introduced into the work area.
The“IVFInseminaon”WitnessPointdoesnotrequireanunassignedtagasthe“Unassigned
tagswillbeassignedto:”pulldowninFigure6-29shows“none”.
Whereawitnesspointrequirestheintroduconofanunassignedtag,usethe“Unassignedtagswillbe
assignedto:”pulldowntoselectthetargettagtype.
Paent ID (female)
IVF Inseminaon
Egg Culture Dish
Inseminated Dish
Figure 6-30 A reassigned tag is represented by a curved line between
the two tag names
Anunassignedtagisrepresentedbyastraightlinebetweenaquesonmarkandthetargettagname.
A witness point may specify both a reassignment and the use of an unassigned tag.
Secon 6
60
RI Witness™ Manager
6

Incoming links to a witness point specify the tags that must be present before the procedure represented
bythewitnesspointcanbeperformed.Ifanunassignedtagisspeciedthenthatcontainermustalso
be present before the procedure can be performed.

Awitnesspointwithmulpleinputlinksofthesametagtypeeg“dishX”,representsanORoperaon,
ie the required container may be sourced from the procedure represented by witness point A OR B.
A B
C
Dish X Dish X Figure 6-31 Dish X must be available to
witness point C
Witness point A OR B may be the source

AwitnesspointwithmulpleinputlinksofdieringtagtypesrepresentsanANDoperaon,iethistag
type AND that tag type are required to perform the procedure represented by this witness point. See
Figure 6-32.
A B
IVF Inseminaon
Sperm Wash Tube Egg Culture Dish
Inseminated Dish
Figure 6-32 The “IVF Inseminaon” witness point
represents a procedure that requires a “Sperm
Wash Tube” AND a “Egg Culture Dish”

Entry(inial,starng)witnesspointshavenoincominglinks.Anentrywitnesspointwillalwaysrequire
anunassignedtagwhichwillbecomeassignedoncompleon.SeeFigure6-33.
Figure 6-33 “Assign Paent ID (female)” is an
entry witness point as it has no input links
An unassigned tag (here an unassigned ID card )
is required
Assign Egg Wash
Egg Wash Dish
Paent ID (female)
Assign Paent ID (female)
? → Paent ID (female)

Checking the box,seeFigure6-29,changesthecompleonsequenceforawitness
point. A second operator must log in to approve the procedure. Entry witness points are generally
double witness points.
Secon 6
RI Witness™ Manager
61

6

Amismatchalarmwillbegeneratediftagsassignedtopaentswhoarenotspeciedaspartnersare
introduced to the work area.
Theexcepontothisruleisprovidedbydonorwitnesspoints.Checkingtheboxwillcreatea
donor witness point which is given a diagonal colouring. See Figure 6-34.
Assign Egg Wash
Egg Wash Dish
Egg Prep
Donate Eggs
Figure 6-34 A known donor witness point

Anincominglinktoadonorwitnesspointspeciesataggeddonaonandcoversproceduressuchas
egg/embryodonaon/sharingaswellassurrogacy.
Whenthewitnesspointisselectedtheoperatorchoosesthedonaonrecipient.Therecipientidenty
isassignedtothetagcontainingthedonatedmaterial.Theworkareanowcontainstagswithamixture
ofdonorandrecipientidenes.Thisdoesnottriggeramismatchbecausethetagshavethedonor
witness point in common.
TaggedDonaonsrequirethatthedonorandrecipientbespeciedassuchviathe and 
checkboxesavailableinthepaentdetails.SeeFigure6-35.
Can Receive Untagged Donations
Can Receive Tagged Donations
Can Donate
First Name
Last Name
Date of Birth
Wendy
Rogers
27 October 1967
Partner 36734M Mr John Rodgers
Figure 6-35
The   checkbox species
theabilitytomakeataggeddonaon.
The    
checkbox must be checked for the
recipient.
The donor witness point ensures
that donated tagged material is only
available to paents that  have  the
    box
checkedintheirpaentdetails.
Secon 6
62
RI Witness™ Manager
6

Spermdonaonsareusuallyuntagged.
An donor point will be an entry witness point for a female recipient of donated sperm. The donated
sperm will be transferred to an unassigned tag that is then assigned to the female recipient when the
donor witness point is selected.
Thedonorwitnesspointensuresthatuntaggedspermisonlyavailabletopaentsthathavethe
boxcheckedintheirpaentdetails.

Aquesonmaybepresentedtoaworkareaoperator.Thequesonandresponsewillberecordedin
the history log.
Tosetaquesonfortheselectedwitnesspoint:
1. Click the buon.
2. Chooseaquesontypeandenterthequesontext.SeeFigure6-36.
3. The diagram will show an appendedtothewitnesspointnameifaqueson
has been added.
4. Click the toeditthequesondetails.
Figure 6-36 Adding a witness point queson that requires a numerical answer
Note the exclamaon aer the witness point name
Paent ID (female)
IVF Inseminaon
Egg Culture Dish
Inseminated Dish
!
!
!
Secon 6
RI Witness™ Manager
63

6

Click   to specify the name and icon for each RFID tagged item used in the witness point
diagram. See Figure 6-37
Figure 6-37 Tag types

From the witness point diagram page click  to specify the order in which points will
be displayed to the work area operator. Click  or  to change the order. See Figure 6-38.
Figure 6-38 Seng the witness point order.

Workareaevents,egwitnesspointseleconandtagmismatches,areloggedintheshareddatabase.
Thewitnesspointlog maybeexplored in detailor viewed as a summary. A printedreport maybe
generated from any view of the witness point log.
Click the mainmenubuonthenclick or  to view the witness point
log. See Figure 6-39.
Secon 6

RI Witness™ Manager
6
Figure 6-39 The witness points screen
Click stascs or explore to view the witness point log

TheWitnessPointExplorershowseventsfromthewitnesspointlog.
Theviewmaybelteredbydate,operator,paentandtagtype.Shortcutlinksareprovidedforcommon
lters,eg“Monday”,“Adminassignsthisweek”.SeeFigure6-40.
Figure 6-40 Exploring the witness point log

Thestascsviewsummarisesthewitnesspointlogbypresenngsignicanttotals,eg“Tagsused”,
“Paentsseen”,etc.Theviewmaybelteredbydate.SeeFigure6-41.
Figure 6-41 Stascs from the witness point log
Secon 7
Cryo Witnessing
65

7


Many embryology labs wish to extend witnessing and reporng to include samples stored in cryo
storage.TheCryofeatureisabarcodelabellingsoluonforcryostraws.
Clinicshavetheoponofscanningbarcodesthattheyhavealreadybeenassignedtosamples,andRI
barcodesthathavebeenprintedfromRIWitness™Managerandareassignedtoaparcularpaent.
IfacliniconlywishestobeabletoscanabarcodeprintedfromtheRIWitness™Managerapplicaon
1. Open .
2. Click 
3. Click 
4. TickthecheckboxundertheBarcodesecon.
Figure 7-1 Only use RI barcodes

1. Open 
2. Fromthepaentdetailswindowclick. See Figure 7-2.
Figure 7-2 RI Witness™ Manager
Secon 7
66
Cryo Witnessing
7
Figure 7-3 Barcode prinng window
3. To print barcodes make sure that a correct label printer is plugged into the computer with the
correct label stock loaded into it.
4. Select
• The required number of barcodes
• Thestarngnumber
• The freeze date of the barcodes.
5. A preview of the barcodes that will be printed is shown in the . The last number
onthesecondlineisthesamplenumberforthispaent.Thiscanbeeditedallowingreprinng
ofaparcularbarcode,forexampleiftheinialprinngwasmisaligned.
6. Click .
7. If you are using small style labels you may need to print a blank row to gain access to the printed
labels. To print a blank row of labels, click .
8. Tochecktheprintedlabels,measuretheYOsetbetweenthetopofthebarcodeandthetopof
thenextlabelandsettheprintheadposionontheprintertothisvalue.
Printheadpos.Y
+7.2 mm
1
2
Figure 7-4 Aligning the label print stock
1. The rst print shows incorrect alignment.
2. Alignment is corrected by seng the
printhead Y posion
Secon 7
Cryo Witnessing
67

7

Intheworkareaapaentcanbechosenbyscanninginasamplebarcodethathaspreviouslybeen
assignedtothispaent.
When compleng a witness point that includes a barcoding step, the barcode, either a brand new
barcodeorabarcodepreviouslyassignedtothecurrentpaent,mustbescannedpriortotheWitness
PointConrmaonscreen.SeeFigure7-5.
Figure 7-5 Scanning a barcode
Ifthebarcodehasnotbeenseenbythesystembeforeitwillbeassignedtothepaentaerthewitness
pointisconrmed.
Ifthebarcodehasalreadybeenscannedandassignedtoadierentpaent,themismatchalarmis
acvatedandthedieringpaentisshownonthemismatchscreen.Clickingonthesmall
nexttothedisplayedmismatchedpaentwillallowthemismatchscreentobeclosedandareason
aached.SeeFigure7-6.
Figure 7-6 Barcode mismatch
Secon 8
68
RI Witness™ Manager for Traceability
8


Materialtypes,egdishes,pipeesandmediamustbedenedforusewithinTraceability.Abarcode
readermaybeusedtoidenfybatchlotnumbers,expirydates,etcorthesedetailsmaybeentered
manually.Batchesareopenedorclosedtoreecttheiravailabilitywithinthelab.
Click the mainmenubuontoviewthematerialsrelatedfeaturesofTraceability.SeeFigure
8-1.
Figure 8-1 Materials management

Materialtypesmustbeconguredbeforethedetailsofadeliveredbatchmayberecorded.
1. Click the mainmenubuon.
2. Then click  to view a list of all material types.
3. Use  and  to specify the top level of your materials hierarchy. See
Figure 8-2.
4. Right click a group and select toextendthehierarchy.
5. Right click a group and select  to add a new material type to that group. See Figure 8-3.
Figure 8-2 Viewing all material types
Secon 8
RI Witness™ Manager for Traceability
69

8
Figure 8-3 Right click to add a sub group

Double click a material type, or right click and select  to view the material type
details. See Figure 8-4.
Figure 8-4 Material type details
SomematerialsaremarkedwithaGS1barcode.AGS1barcodemaycontainvariouscombinaonsof
expirydate,lotnumberandauniqueproductidenerknownasaGTIN.Hereweareonlyinterested
inaproductidener.
1. If you have a barcode, click  and scan the barcode.
2. Manually enter a unique product part number if no GTIN is available.
Secon 8
70
RI Witness™ Manager for Traceability
8

GS1isaglobalbarcodestandardthatmaycontainaUniqueProductIdener(GTIN)andmayalso
containlotnumberandexpirydateinformaon.Someproductshaveabarcodethatdoesnotadhere
totheGS1standard.SomeGS1barcodesdonotcontainalltheinformaonhighlightedabove,egabox
ofbolesmayhaveabarcodethatcontainsGTIN,lotandexpiryinformaonbutanindividualbole
barcodemayonlyhaveLotandExpiryinformaonbutnoGTIN.
Traceability can interpret GS1 barcodes.
A Traceability buon must be clicked to interpret a GS1 barcode, eg click   or   or
.

Treatmentcycletypesmustbeconguredandlinkedwithmaterialtypes.
1. Click .
2. Then .
3. Then the  tab to view the materials linked to a cycle type.
4. Tick the materials within the list that are to be associated with the treatment cycle type.
5. Press .
Aspreviouslydescribedin“Secon6-RIWitness™Manager”onlyadministratoroperatorsmaycreate
cycletypes. Traceabilityadminoperatorsmayaddionallyconguretherangeofmaterialsthatare
used when performing a treatment cycle type.
Anewlycreatedcycletypewillnotbeassociatedwithanymaterials.Usethematerialscheckboxesto
specify the range of materials that will be used for the selected cycle type. See Figure 8-5.

A new batch of a GTIN barcoded material may be scanned directly into Traceability.
1. Click themainmenubuonandthenclickthebuon.
2. Youwillbepromptedtousethebarcodescannertoreadthebarcode.Scanthebarcode.
3. As the materials type is already logged in the system, it will recognise the GTIN barcode and add
thenecessarydetailsofthatbatchofmaterialstothesystem,eglot,expirydate,etcwithinthe
Batch Details page.
Materials that are not GTIN barcoded are selected by navigang to the appropriate “material type
details”screen.
1. Click the mainmenubuonthenclick to show a list of all materials.
2. Double click the material that is being processed to show the Batch Details page for that
material. See Figure 8-7.
3. Alistofexisngbatchesofthismaterialisshown.Click to open the batch
detailswindowforanewlycreatedbatchandllinthebatchdetails.SeeFigure8-6.
4. Click  to create a new batch if a GS1 barcode is available.
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
8
Figure 8-5 Material type details checkboxes specify the range of materials associated with each cycle type
Figure 8-6 Creang a new batch by scanning a barcode
Secon 8
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RI Witness™ Manager for Traceability
8

Normalbatchoperaonsarestatuschanges,eg
• : The batch of materials will shortly be opened and used
•  The batch of materials has been opened and is in use within the lab
• Thebatchofmaterialshasbeenclosed,hasallbeenusedup,hasexpired,cannolonger
be used.
Batchesarenotrounelydeleted.

If a batch is created in error then an admin operator may delete it.
1. Click the mainmenubuon.
2. Click .
3. Double click the material type to be deleted.
4. The list of batches will be shown. Right click the appropriate batch and select .

Anewlycreatedbatchwillbeassignedastatusof“Pending”.Click when the batch is made
availableforuse.Thedetailsdisplayedforanopenbatchwillincludeallpaenttreatmentcyclesthat
are in progress. See Figure 8-8.
When a batch is no longer in use, click tochangethebatchstatusfrom“Open”to“Closed”.

If a batch is opened in error, click the buon toreturnthebatchstatusto“Pending”.
If a batch is closed in error, click the buon toreturnthebatchstatusto“Open”.
Figure 8-7 Lisng all batches of a specic material type
Click “Add Batch” to manually create a batch
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
8
Figure 8-8 Paent treatment cycles related to a batch of materials

Whenbatchesarecreatedanexpirydateisrecorded.Ifanycurrently“Open”or“Pending”batchesare
expiredorwithin7daysofexpirythenanexpirybuonwillbeavailableonthemainMaterials
screen. See Figure 8-9.
Doubleclickanexpiredbatchtoshowthebatchdetailsscreenfromwhichthebatchmaybeclosed.
Now remove the batch from use.
Theexpiryperiodcanbesettoyourrequiredmeperiod,ieextendedto10daysorshortenedto5
days.
1. Click in the main menu.
2. Click .
3. WithintheMaterialsseconamendthebatchexpirywarningeld.
Figure 8-9 Expired and expiring batches
Secon 8

RI Witness™ Manager for Traceability
8

From the main Materials screen click toviewallbatches.Alteredlistofallbatchesisshown,
expiredbatchesarehighlightedinred.Thislistmaybelteredbydateandbybatchstatus.SeeFigure
8-10.
Figure 8-10 A ltered view of all batches

See“Secon5-RIWitness™Basicoperaon”fordetailsofcreangandmanagingatreatmentcycle.
When viewing the details of a treatment cycle Traceability operators may click the Materials tab to
show material batches related to this cycle. See Figure 8-11.

Batchescanbeexcludedfromcyclesbyrightclickingonthebatchandchoosing on the
contextmenu.
Excludedbatchescanbeviewedorhiddenbyclickingthebuonabovethe
batcheslist.Excludedbatcheswillbehighlightedblue.
Anexcludedbatchcanbeincludedagainbyrightclickingonanexcludedbatchandfromthecontext
menuleclicking. The batch will now be visible in the list of batches for the
cycle.
Figure 8-11 Material batches related to this cycle
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
9


Hand wrien notes are oen taken whilst performing laboratory procedures. For example embryo
scoresandspermvolumesmightbewriendownonadatasheetandlatermanuallytranscribedinto
aclinicferlitydatabase.
The features of Data Capture described in this chapter allow data sheets to be designed in RI Witness™
Manager and data entry to be performed in the laboratory using the work area touch screen. Data entry
may also be performed in RI Witness™ Manager.

1. Click 
2. Then click toenterthesetupscreenfordatacollecon.Sheetdesignis
performed using this screen.
The Collected Data Setup screen, Figure 9-1, is divided into 4 regions. Note that region 4 shows the
properesofanentryselectedfromregions1,2or3.
Figure 9-1 Collected data setup
1
3 2
 
Region 1 shows a list of all the sheet types that have been created. Click  to add a new sheet,
setthenameofthenewsheetusingtheproperesinregion4.
 
Parametersareeldsthatmaybeplacedonadatasheet.Forexample,aparameternamed
“SpermVolume”thatrequiredanumericalvaluemightbeaddedtoan“Andrology”datasheet.
Region 2 lists the parameters that have been created and may be added to the contents of a
sheet.
Click the buontoaddaparametertoasheetcontents.Theparameterwillmovefrom
region 2 to region 3.
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Data Capture
9
 
Region 3 shows the content layout of the selected sheet type. The contents of a sheet are
parameters that have been added to the sheet from region 2. To change the layout order,
highlight the parameter by clicking it. Then use the  and buonstoposionitinthe
order required.
Onceaparameterhasbeenaddedtoasheetcontents,addionalaributesmaybeset.For
example,andefaultvaluecanbesetoraparametercanbemarkedasrequiredsothatan
operator must provide a value.
See“ParameterAributes”onpage87formoredetails.
 
Theproperesshowninregion4reectthemostrecentlyselectedentryfromregions1,2or3.
See Figure 9-2.
Figure 9-2 Examples of parameter properes and sheet properes shown in region 4

The contents of a sheet are called parameters.
1. Click intheparametersregion(2)tocreateanewparameter.Theproperesregion(4)will
show details of the newly created parameter. See Figure 9-3.
2. Enteranameforthenewparameter,eg“SpermVolume”.
3. Therstacontoperformonanewlycreatedparameterwillbetoselectaparametertypefrom
theProperesTypedropdownmenu.Parameterproperesdependontheselectedparameter
type.Forexamplethetexttypehasproperesminandmaxlengthwhereasacalculaonhas
twooperandsandanoperaon.
Figure9-3showstheproperesofeachparametertype.Figure9-4showstheParameterTypedrop
down menu.

Theproperesofanumericalparameterincludeaminimumvalue,amaximumvalueandanoutput
format.Theformatspeciesthenumberofdecimalplacestobeusedwhendisplayingnumbers.
Format meansthatthevalueenteredbyanoperatorwillnotbeformaed.Figure9-5showsthe
Format pull down.

Theproperesof a textparameter include a minimum length and a maximumlength.Leavethese
properes blank if length restricons are not required when the operator enters a value for this
parameter.
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
9
Figure 9-3 Parameter properes depend on the selected parameter type
Select from: Number, Text, Check, List, Date, Time, Date Time, Built In and Calculaon
Figure 9-4 Selecng a
parameter type
Figure 9-5 Selecng a
numerical format
Figure 9-6 The properes of a list
parameter

Listparametersallowtheoperatortochooseavaluefromapre-denedlist.Theproperesofalist
parameter are shown in Figure 9-6.
Click the buontoshowtheManageListswindow.TheexampleshowninFigure9-7highlights
the“PolarBodyPosion”listthatcontainsthe3values,  and .The“Polar
BodyPosion”listisassignedtothe“”listparameter.SeeFigure9-6.

CheckparameterstakeYes/Novalues.
SheetspresentedbyRIWitness™Managerwillshowcheckboxes.Aworkareatouchscreenwilloer
aseleconof or .
Secon 9
78
Data Capture
9
Figure 9-7 The manage lists window Figure 9-8 Seng a date me value
Figure 9-9 Adding two numbers using a calculaon parameter Figure 9-10 Selecng a built in value

DateandmevaluesareviewedandeditedusingRIWitness™Manager.Anoperatormaytypeavalue,
eg 12:30 or 14/08/2010 15:35. A date picker window is also available. Figure 9-8.

Calculaonsperformanoperaonontwooperands,egaddtwonumbersorjointwopiecesoftext.
Theoperandsofacalculaonmaybeparametersofanytypeincludingcalculaontypes.Aexample
calculaonisshowninFigure9-9.

Builtinparameterswilldisplayvaluesderivedfromtheassociatedtreatmentcycle,egtheeggcollecon
date. Figure 9-10 shows the available built in values.

Click the parameters buontomoveparameterstoasheetcontents(region3).Intheexample
shown in Figure 9-11, four list parameters have been added to the contents of the 
sheet.
Figure 9-12 shows another example of sheet contents where two list parameter and four number
parameters form the contents of the Sperm Pre Prep sheet.
Secon 9
Data Capture
79

9
Figure 9-11 Adding parameters to the Embryo Development sheet
Figure 9-12 Adding parameters to the Sperm Pre Prep sheet

A parameter may be part of the contents of many sheets. The same parameter may be editable on
sheet A and sheet B and but not editable on sheet C. A parameter that appears on many sheets has
asinglesharedvalue.IntheaboveexampleavaluechangeonsheetAwouldautomacallyappear
changed on sheets B and C.
Thecontentsofasummarysheetmighttakeparametersfrommanyothersheets.See“CycleSummary
Sheets” on page 86 for more details. See “Parameter Aributes” on page 87 for more details of
theeditable/noneditableparameteraribute.
Secon 9
80
Data Capture
9
Figure 9-13 Use the checkboxes to associate sheets with the selected treatment cycle type

Sheets may be viewed and edited on a laboratory work area touch screen.
If sheets have been associated with the treatment cycle type being performed, then a  
buonwillbeshownonthetouchscreen.SeeFigure9-14.
Touch the buontoseealistofavailablesheets.Touchabuontovieworedit
the sheet values. See Figure 9-15 and Figure 9-16.
Use the touch screen  and arrowbuonstohighlightthesheetvaluetobeedited.Nocethat
thetypeofthevaluerequiredisindicatedtotheleofthevalueentrybox,eg123fornumericalinput,
ABCfortextinputandindicanguseofthearrowkeysforlistandYes/Noinput.

Treatmentcycletypes,egIVF,ICSIorIUI,willhavedierentdatacolleconrequirements.Forexample,
a sheet designed for IUI would not be used during an ICSI treatment.
1. Click  then  to associate sheet types with a treatment cycle type.
2. TheDataCollecontablistsallavailablesheets.Checktheboxesofthosethatareassociated
with the selected cycle type. See Figure 9-13.
3. Click .
Whenalistofsheetsispresentedtoanoperator,theywillbeorderedasspeciedhere.Usethe and
buonstochangethatorder.

During a treatment cycle an operator may view and edit the values on a sheet. Each treatment cycle
will start with blank copies of the sheet types that have been associated with the treatment type being
performed.
Secon 9
Data Capture
81

9
Figure 9-14 Touch the “Data Sheets” buon to view or edit all sheets associated with this treatment
Figure 9-15 Touch buons for each available sheet are shown in the work area
Figure 9-16 Use the touch keyboard to edit sheet values
Secon 9
82
Data Capture
9

Data values will most likely be entered at the work area touch screen but sheets may also be viewed
and edited using RI Witness™ Manager.
1. Selectapaent.
2. Click the  tab and then double click a cycle to show the Treatment Cycle
screen.
3. Click the  tab to view or edit any sheet associated with this treatment shown in Figure
9-17.

TheproperesofaparameterincludeasengthatdefaultstoCycle,ieatreatmentcycle
doesnothavemulplevaluesforthisparameter.SeeFigure9-18.
However many parameter values will relate to an individual egg. Change the sengfrom
“Cycle”to“Egg”forsuchparameters.Eggrelatedparametersaredisplayedtotheoperatorasatable
containing one row for each egg. See Figure 9-19 and Figure 9-20.
On the work area touch screen, touch a row of the egg table to edit the values for that egg. See Figure
9-21. The circulararrowbuonsatthetopof thescreenmaybeused toselect anotheregg
without returning to the table view.
Figure 9-17 Eding sheet values with RI Witness™ Manager
Figure 9-18 The relates to seng defaults to cycle
Secon 9
Data Capture
83

9
Figure 9-19 A RI Witness™ Manager view showing a table of egg related parameters.
Figure 9-20 A RI Witness™ work area touch screen view showing a table of egg related parameters
Figure 9-21 Entering values for egg number 1 at the work area touch screen
Secon 9

Data Capture
9

1. Click .
2. Then to view a list of treatment types.
TheDataCollecontabisusedtoassociatesheettypeswithatreatmenttypeasdescribedearlierinthis
chapter.TheDataCollecontabalsooersanEggCountParameterseng.Thedropdownmenufor
the Egg Count Parameter is a list of all parameters. Select one parameter that will contain a numerical
value. The selected parameter will control the number of rows in any table of egg related parameters.

The parameter selected to represent the egg count can be any parameter with a numerical value that
relatestoacycle.InFigure9-22theparametercalled“TotalNumberofEggs”hasbeenselectedfrom
a list of all parameters.
Asanexample,“TotalNumberofEggs”mightbeacalculaonparameterwhoseoperandsareother
parameters. See Figure 9-22.
Figure 9-22 The number of eggs involved in a treatment is set using the egg count parameter
Figure 9-23 This calculaon parameter has been selected as the egg count parameter in the example
shown in Figure 9-22

Changes to the value of the parameter set as the  will change the number of rows
in any table of egg related parameters.
Ifthenumberofeggsisincreased,thenthenumberofrowsinalleggtableswillautomacallyincrease.
Ifthenumberofeggsisdecreased,thenthenumberofrowsinalleggtableswillnotautomacally
decrease. A decrease in the number of eggs will be indicated in RI Witness™ Manager as an 
. See Figure 9-24.
Secon 9
Data Capture
85

9
A conict resoluon window is shown when the    buon is clicked. See
Figure9-25.Theconictresoluonwindowallowstheoperatortomanuallyselecttheeggrecordsthat
willbedeletedtoachievetherequireddecreaseineggcount.Theconictwillberesolvedwhenthe
number of egg records equals the value indicated by the Egg Count Parameter.
If it is decided that a decrease must be “undone”, then click the  buon to exit the conict
resoluonwindowandincreasethevalueoftheEggCountParametertomatchthenumberofegg
records.
Figure 9-24 The Egg/Embryo Count Conict buon is shown if the value of the parameter selected as the egg
count parameter is decreased
Figure 9-25 The Egg/Embryo Count Conict window
Secon 9
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Data Capture
9

The work area touch screen buonoersaccesstoallthedatasheetsthathavebeen
associated with a treatment type.
Amoreselecveapproachtodatacolleconispossiblebylinkingasheettypetoawitnesspoint.See
Figure 9-26.
When a touch screen buonistouched,thelinkedsheetwillbeautomacallypresented
so that the operator may enter data related to the procedure being performed. See Figure 9-27.
Figure 9-26 The selected witness point “IVF Inseminaon” is linked to the data collecon sheet
“ 4 Inseminaon/ICSI”, see above at boom right
Figure 9-27 A witness point will trigger the presentaon of a linked data collecon sheet

Any sheet type may be selected as the cycle summary sheet.
1. Click  in the main menu bar.
2. Then click .
3. Use the  drop down menu to select the sheet type that will become the
summary sheet for this treatment type. See Figure 9-28.
Secon 9
Data Capture
87

9
Figure 9-28 Select one sheet to become the cycle summary sheet
Figure 9-29 Touch the cycle summary buon to see the cycle summary sheet
Touch again to return to the original display
Atypicalsummarysheetwouldshowparametervaluesfrommanyothersheets.See“SharedBetween
Sheets”onpage79formoredetails.
Quick access to a read only view of the cycle summary sheet is visible in the work area via the touch
screen Buon.SeeFigure9-29.

Parameters that have been added to a sheet contents may be given sheet specic aributes. Click
 then . See Figure 9-30.

Click in the column and enter a value.
Inialvaluesarelinkedwiththe and checkboxes.
Aninialvaluesengwillbeignoredunlessacombinaonof and or  is also checked.
See Figure 9-31.
Secon 9
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Data Capture
9

Thevalueofeditableparametersmaybechanged.Ifthischeckboxisnotcheckedthentheparameter
willbeshown“greyedout”andmaynotbechangedonthissheet.

Anoperatormustenteravalueforarequiredparameter.Thesheetcannotbesavedunlavalueis
entered. Required parameters are highlighted with an asterisk on the work area touch screen and in
red when viewed in RI Witness™ Manager.
The value may not be edited
n/a
Edingallowedbutmaybeleblank.
Avaluemustbeenteredbutitmaynotbeleblank.
Ifavaluealreadyexistsitwillbeoverwrienwiththeinialvalue.Theusercannotchangethevalue.
n/a
Ifavaluealreadyexistsitwillbeoverwrienwiththeinialvalue.Theusermaychangethevalue.
Ifavaluealreadyexistsitwillbeoverwrienwiththeinialvalue.Theusermaychangethevaluebutitmaynotbe
leblank
Ifnovalueexiststhenshowtheinialvalue.
The user may not change this value.
n/a
Ifnovalueexiststhenshowtheinialvalue.
The user may change this value.
Ifnovalueexiststhenshowtheinialvalue.
Theusermaychangethisvaluebutitmaynotbeleblank.
Theinialvaluebeshown,overwringanyexisngvalue.
The user may not change this value.
n/a
Theinialvaluebeshown,overwringanyexisngvalue.
The user may change this value.
Theinialvaluebeshown,overwringanyexisngvalue.
Theusermaychangethisvaluebutitmaynotbeleblank.





Whatdoesthiscombinaonmean?
Figure 9-31 Combining parameter aributes
Figure 9-30 Seng parameter aributes.
Inial Value, Editable, Overwrite, Create and Required
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
10


A camera will need to be connected to the PC running RI Witness™ and the drivers installed correctly
toviewliveimageswithinRIWitness™.TheImagingsecuritydonglemustalsobeedtoaUSBport
and a valid Imaging license issued.
RIWitness™sowarewillautomacallyrecognisebothanalogueandcompabledigitalcameradevices
connected to the PC. If there is only one camera device detected, it will show the live image for that
device.Ifmulplecameradevicesareconnected,itwillusethecamerathatwaslastselectedfromthe
videosourcedropdownbox.
IftherearemulplecamerasaachedtothePC,thedeviceyouwanttheliveimagetobeviewedfrom
may be selected.
To do this, select the Camera from the dropdown list on the  panel.

MulpleImagingscreenscanbeconguredforusewithImaging.
1. Open 
2. Unlocksengs.
3. Touch .
4. Setyourdesiredconguraon
Iftheworkareaiswindowed,thenmulpledisplayswillnotbeshown.Windowedworkarea
isrecommendedforAdminPCswithreadersconnected.Toenablesimplyckthe
box.
5. ThecurrentoperatorandpaentwillbedisplayedonthesecondaryImagingscreen.
BothoperatorsandpaentsmaybechangedbyclickingtheappropriateareaontheImagingscreen.
Imagesarecapturedforthepaent’scyclecurrentlyacveintheworkarea.Imagescapturedforthe
acvecyclewillbedisplayedintheRIWitness™Managersortedbydateandme.
Figure 10-1 Combining parameter aributes
Figure 10-2 Imaging screen showing operator and paent
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10
Ensurethatthereisalocaonsettostorecapturedimages.ThisissetinRIWitness™Manager.
1. Click in the Main Menu tool bar.
2. Click .
3. SetthedesiredlepathwithintheImageStorageLocaoneld.
Figure 10-3 Images captured shown in the RI Witness™ Manager screen
Figure 10-4 Image storage locaon

This is available on both the work area screen and secondary Imaging screen.
1. Log in using your operator name and PIN.
2. Ensurethereisapaentwithanacve/opencycleintheworkarea.
3. Press the  icon .
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10

This is available on both the work area screen and secondary Imaging screen.
1. Login using your operator name and PIN.
2. Ensurethereisapaentwithanacve/opencycleintheworkarea.
3. Click the  icon .
4. Eachvideorecordingcanlastforamaximumofonehour.Thiseliminatestheproblemof
accidentallyleavingthesowarerecordingandpossiblyrenderingthecomputerunusable.
5. When freeze frame is set as keyboard shortcut, the live image recording can be paused. A
second press of the shortcut key will restart the live image recording.
6. Press or click  to stop recording.

Available on the Imaging screen
There are three ways to zoom into an area on the screen.
1. Placethemouseatapointonthescreen.Clickandholdtherightmousebuon,thenrelease.
This will zoom to the preset zoom level into the spot where the mouse is placed. To zoom out,
clickandholdtherightmousebuon.
2. Place the mouse at a point on the screen and use the scroll wheel on the mouse (if available).
This will give the user control over how much digital zoom is given.
3. Usingthetoolbarattheboomofthescreen,clickthe+ icon to zoom in incrementally. To
zoom out by the same amount, click on the icon on the tool bar. The magnifying glass icon will
zoom in and out by the preset zoom.
Whilstusingdigitalzoom,athumbnailimageofthescreenwillappearinthetopleofthescreen.At
thetopofthispanelistheamountofmagnicaonyouareusing.Whenzoomedoutfully,thispanel
will not appear on the screen.
Zooming in and zooming out can also be achieved using the foot pedal.

Available on the Imaging screen
1. Click  icon on the tool bar.
2. Click on the screen to select a start point for the measurement.
3. Clickonthescreenasecondmetoselectanendpointforthemeasurement.
The ends of the line can be dragged to change the measurement.
Linescanberemovedbyclickingthecrossinthecontextwindow.
Duringlaseroperaon,linesareshownbutcannotbedragged/createdandnomeasurementswillbe
displayed.
Lines and measurements are shown on an image when images are taken in Line Mode.
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10

Available on both the work area screen and secondary Imaging screen.
1. Open 
2. Unlocksengs.
3. Press
4. Click on thetoallowtheseleconofcameras.Clickingontherequiredcamera
in the list will change the live image to that of the selected camera.

Available on both the work area screen and secondary Imaging screen.
1. Open 
2. Unlocksengs.
3. Press
4. Tick (forcameradevicesthatsupporttheseopons).
5. Tick (forcameradevicesthatsupporttheseopons).

Available on the Imaging screen.
1. Click the  icon from the Imaging screen.
2. Drag the Preset Zoom slider to the required zoom.

Available on both the work area screen and secondary Imaging screen.
1. Open 
2. Unlocksengs.
3. Press .
4. Clickthecorrespondingeditbuontoassignashortcut.
5. Hold down the key or press the foot pedal to assign.
6. Click or press the  to save the shortcut.
Oncetheshortcutsaresetup,presstheshortcutkeysorthecorrespondingfootpedaltoacvatethe
command.

Available on the Imaging screen.
1. Click the  on the tool bar.
2. Clicktherequiredobjecvemagnicaon.
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10

Available on the Imaging screen.
1. Click on the tool bar.
2. Click the buon.
3. Click Themagnicaonwillbethesameasthecurrentmagnicaon.
4. Clicktherequiredobjecvetoeditobjecvemagnicaonandneadjustmentvalues.

Available on the Imaging screen.
1. Click on the tool bar.
2. Click the buon.
3. Click Xnexttoanobjecve.

Available on the Imaging screen
1. Click on the tool bar.
2. Click the buontoopentheObjecveCalibraonpanel.
3. Place an object of known dimensions (a stage micrometer is supplied with each system for this
purpose)intheeldofview.
4. EnsurethattheobjecveselectedinImagingmatchesthatbeingusedonthemicroscope.
5. Thestagemicrometersuppliedmeasures100μmbetweenthelongerlines.Forbestaccuracy,
posioneachendpointofthelineatexactlythesamerelaveposiononthescale,for
exampleattherighthandedgeofeachvercalline.
6. Drag the rulers to the point where the stage micrometer is going to be measured from and use
theneadjustmentupanddownunltherulerscalematchesthestagemicrometer.

1. Tocongureascreenasapaentdisplaysimplyselectthe for the
desiredscreen.ThescreenwilldisplaytheRIlogounlthedisplayisturnedon.
On O
Figure 10-5
2. Toturnthepaentdisplayon/osothatitshowsthevideofromthecamera,clickthe or
buoneitherontheworkareaoronthepaentdisplaypanelontheImagingdisplay.The
Imagingdisplayshowsapreviewofwhatthepaentdisplaywillshow.SeeFigure10-5.
3. Totakeasnapshotofthevideotouseonthepaentdisplay,pressthebuononthe
workareaorpaentdisplaypanel.
4. To re-enable live streaming of the video, press .
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Imaging
10
Whenacurrentpaentisacvatedviatheworkarea,thepaentnameandIDwillappearonthe
PaentDisplay.Itwillonlybevisibleifthepaentdisplayisturnedon.SeeFigure10-6.
5. Closingthemainwindowwillalsoclosethepaentdisplay.

RIWitness™canberunalongsideandinconjunconwithSaturn™LaserSystems.Pleaserefertothe
Saturn™LaserSystemusermanualforfullinstrucons.

Toaccesssengsandinformaonfromtheworkarea
1. Open 
2. Unlocksengs.
3. DetailedinformaonabouttheSaturn™LaserSystemwillbedisplayed.
4. Press  icon toenableBiopsyorMul-PulseModes.
Figure 10-6 Informaon shown on paent display
Figure 10-7 Saturn™ Laser System informaon
Secon 11
Troubleshoong
95

11

  
Tags Reading
IntermientlyorOnly
in Certain Areas
RF noise Many devices in the lab can cause RF noise.
ContactRIoraRIrepresentave.
Antenna not tuned
Metal objects may change antenna tuning.
Posiontheantennawhereitwillbeused.If
the antenna that is not tuned is an auto tuning
antennafollowtheinstruconsshownin“RF
Calibraon”onpage19,otherwisecontactRI
oraRIrepresentavetoretunetheantenna.
Antenna not tuned
Metal objects may change antenna tuning.
Posiontheantennawhereitwillbeused.If
the antenna that is not tuned is an auto tuning
antennafollowtheinstruconsshownin“RF
Calibraon”onpage19,otherwisecontactRI
oraRIrepresentavetoretunetheantenna.
Looseconnecon Checkforanycablesunpluggedornotghtened
fully.
Control unit / antenna /
mulplexerfaulty ContactRIoraRIrepresentave.
Tags Not Reading
Broken tag Checkthetagonadierentantenna.
Tag not encrypted
Use the . Non encrypted tags
are shown in red.
RF noise Many devices in the lab can cause RF noise. Contact
RIoraRIrepresentave.
WorkAreaconguraon
Use the   to check that the
number of antennas matches the number in
the work area. Also check that the temperature
controllerisspeciedcorrectly.
Antenna not tuned
Metalobjectsmaychangeantennatuning.Posion
the antenna where it will be used. If the antenna
that is not tuned is an auto tuning antenna follow
theinstruconsshownin“RFCalibraon”onpage
19, otherwise contact RI or a RI representave
to retune the antenna.
Looseconnecon Checkforanycablesunpluggedornotghtened
fully.ContactRIoraRIrepresentave.
Control unit / antenna /
mulplexerfaulty ContactRIoraRIrepresentave.
Secon 11
96
Troubleshoong
11
  
Mulplexer / Control
Unit / Temp Controller
Missing
RI Witness™ WorkArea
conguraon
Use the   to check that the
number of antennas matches the number in
the work area. Also check that the temperature
controllerisspeciedcorrectly.
Looseconnecon Checkforanycablesunpluggedornotghtened
fully.
Control unit / antenna /
mulplexerfaulty
ContactRIoraRIrepresentave
Database Missing
RI Witness™ WorkArea
conguraon
Use the  to check that database
connecon is specied correctly. Contact your IT
department for assitstance.
Looseconnecon Check for any unplugged or loose network cables.
Replace with a known good cable.
Secon 12
Temperature Calibraon
97

12


Beforecheckingthetemperatureprole,itisimportanttoestablishifthereaderisaoneortwochannel
reader.Thiscanbedonebyturningothecontrolboxandrestarng.Atthebeginningofthestart-up
procedurethecontrolboxscreenwillshowackoracrossnexttothechannelnumber(seeheated
controlboxdisplaywarnings).Ifthereaderisasinglechannelitwilldisplayacknexttochannelone
andacrossnexttochanneltwo,whilea2channelwilldisplayacknexttoboth.
Thetemperaturegradientovertheheatedplateshouldnotexceed1.5°C.Inasinglechannelreader
the temperature dierence between the centre of the glass window and the highest temperature
measuredawayfromthewindowshouldnotexceed3.0°C.Foradualchannelreaderthetemperature
dierencebetweenthecentreoftheglasswindowandthehighesttemperaturemeasuredawayfrom
thewindowshouldnotexceed1.5°C.

 Follow this guide to posion small amounts of thermal paste at each point to be measured.
 Complete the form ‘RI Witness™ Heated Antenna Service Record’ for each heated reader. The latest
formsareavailablebycontacngRI.
 Use a thermometer probe secured with masking tape to measure the temperature at each of
the 14 points. Allow 3-5 minutes for the probe reading to stabilise at each measurement point.

TheobjecveoftemperaturecalibraonistoensurethatthetemperatureindicatedbytheRIWitness™
sowareiswithin0.2°Cofthetemperaturemeasuredonaheatedreadersurface.
Werecommendusingathermometercalibratedat37°Cedwithasmallthermocoupleprobe,such
as the RI IVF Thermometer.
Ensure that the work area setup is as it will be used, eg microscope light on, fan on, etc.
Figure 12-1 Temperature prole template
Secon 12
98
Temperature Calibraon
12

 Switch the control unit ON.
 Make surethe controlunit RF powerisswitchedo toavoidinterferencewithathermometer
reading.
 Place a small amount of thermal compound 5cm to the right of the reader window. Posion a
thermometer probe in the compound and secure the probe with masking tape.
 Waitunlthethermometertemperaturestabilises.Thiscantakeupto45minutes.
 Compare the temperature shown in the work area soware with the thermometer reading.
Adierencewithin+/-0.2°Cisacceptable.
 If the readings are outside the above requirement then click  to open the temperature
oset sengs and make adjustments. Increase the oset to increase the displayed reading,
decreasetheosettodecreaseit.
 Allowasmalldelayfortheosetchangetoregister.
Figure 12-2
Secon 12
Temperature Calibraon
99

12
Figure 12-3 Touchkit : USB controller screen shot

Thetouchscreencursorshouldaccuratelytracktheposionofanoperatornger.Ifaccuracyproblems
areexperiencedthenuseamousetoacvatethecalibraonulityandperformthefollowingchecklist.
1. Double click the  desktop icon, then click  on the  tab.
2. The touch screen shows a blinking Xinthelowerlecorner.TouchandholdtheblinkingXunlit
stopsblinkingandmovestothenextcornerposion.Repeatthetouchandholdforeachcorner
ofthescreen.Oncompleona“calibraonnished”messageisdisplayed.
3. Touchthescreeninafewrandomlocaons.Repeatthecalibraonifthecursorisnotcorrectly
posioned.
Secon 13
100
Care and Maintenance
13


RIWitness™readersmaybecleanedwithasoclothandmilddetergent.Areadermaybeliedand
returnedtoitsoriginallocaon.Donotdisconnectthecablesaachedtoanyreader.
Do not use solvents for cleaning.
Do not disconnect readers.
Donotchangetheposionofreaders.
Secon 14
Repairs and Returns
101

14


AssumingRIWitness™isregularlymaintainedandrounelyserviced,itshouldperformasrequiredfor
aminimumof7yearsconnualuse,aerwhichmewerecommendyouconsideritsreplacement.
Shouldyounoceimpairedperformanceand/oranyissueswheresafetyiscompromised,orhaveany
otherconcernsduringtheuseofRIWitness™,seektheadviceofRIortheirauthorisedrepresentave
promptly.

In the event that you have a problem with a RI instrument, please follow the procedure below to ensure
promptaenon.
1. Readthe‘Troubleshoong’secon.
2. If you require any further help contact your distributor or RI directly. RI will try to resolve the
problem as quickly as possible.

If the product is no longer serviceable it must be sent back to RI to be destroyed in an
environmentally safe way. Do not dispose of RI Witness™ products with ‘normal’ waste.

1. ContactRItoobtainaReturnedMaterialsAuthorisaon(RMA)number. Goods will not be
replacedorrefundedwithoutprioragreementandclearlystangtheRMAnumber.
2. Pack the item carefully in its original packaging. RI will not accept responsibility for damage due
toincorrectpackaging.Replacementitemsoraddionalrepairswillbeinvoiced.
3. ClearlylabelthepackagewiththeRMAnumber,markthepackage“Urgent-ReturnedItemsFor
Repair”,andshiptotheaddressonthenextpage.Goodsshouldbeinsuredfortheirfullvalue
during shipping.



Tel:+44(0)1326372753Fax:+44(0)1326378783
E-mail: service@research-instruments.com
Website: www.research-instruments.com
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IncompliancewiththeEuropeanMedicalDeviceDirecve93/42/EECasamended,itisyourdutyto
informRIifyoubelievethisdevicehas,ormayhave,causedorcontributedtothedeathofapaentor
userortoaseriousdeterioraonintheirstateofhealth.
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Repairs and Returns
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feedback.Ifyouhaveanysuggesonsforhowwecanimproveourproductsortheinformaonwe
provide with them, please send them to feedback@research-instruments.com.Yourfeedbackwillhelp
usdeveloptheproductandsupporngmaterialstomeetyourfutureneeds.
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