Agilent InfinityLab 2D-LC Solutions. Quick Installation Guide. Contents. Introduction 2. Delivery checklist 2. Options 3. Recommendations for instrument setup 5.
Agilent InfinityLab 2D-LC Solutions Quick Installation Guide Contents Introduction 2 Delivery checklist 2 Options 3 Recommendations for instrument setup 5 General Information 5 Single Heart-Cutting Configuration 7 Multiple Heart-Cutting Configuration 7 Recommended stack setups 8 Alternative instrument setups for additional functionality 12 Installation of the 2D-Valves 19 Attaching the external valve drives 19 Connecting the 2D-LC Valve, Standard (G4236A) 20 Connecting the 2D-LC Valve, ASM (G4243A) 22 Installing the Pressure Release Kit 24 2D-LC Software Configuration 26 Agilent Technologies Introduction Introduction One of the strengths of the Agilent InfinityLab 2D-LC Solutions is its possibility to build systems choosing from a large set of LC modules. Many different modules can be used and even existing systems can be upgraded. The only requirement is a 1290 Infinity Binary Pump G4220A or 1290 Infinity II High Speed Pump G7120A as second dimension pump and a unit containing an Agilent 1290 Valve Drive (G1170A) plus a valve suitable for 2D-LC. The final stack configurations and necessary parts depend on the 2D-LC-Method. Further details are provided in the 2D-LC Solution Manual (G2198-90501) Delivery checklist The Capillary Kit 2D-LC, 1290 Infinity II (5067-6585) contains the following parts: # p/n 2 5043-0269 1 5067-4608 2 5067-4651 1 5067-4669 1 5067-4670 1 5500-1217 1 5500-1227 1 5500-1240 2 5500-1245 2 5500-1251 Description Adapter-profile for Agilent 1290 Valve Drive (G1170A) Capillary ST 0.17 mm x 280 mm SX/S Capillary ST 0.12 mm x 280 mm SL/SX Capillary ST 0.12 mm x 600 mm S/SL Capillary ST 0.17 mm ID 600 mm pre-swaged Capillary ST 0.17 mm x 900 mm SI/SX ps-ps Capillary ST 0.17 mm x 150 mm SL-SL Capillary ST 0.17 mm x 105 mm SL/SL Capillary ST 0.17 mm x 400 mm SI/SI Capillary ST 0.12 mmX 400 mm SL/SL NOTE Depending on the set up of you instrument, extra parts and capillaries might be required for instrument set up. Those parts are ordered separately or are shipped with other components, for example the 2D-LC or MHC valves. Their origin as well as their function is described in the instrument setup section below. 2 Options Options NOTE The Agilent InfinityLab 2D-LC Solutions must contain an Agilent 1290 Infinity Binary Pump G4220A or Agilent Infinity II High Speed Pump G7120A as 2nd dimension pump. This is necessary to achieve the following: · Synchronize valve switches · Run fast gradients on the 2nd dimension column Table 1 Overview of recommended hardware configurations Function in 2D Functional Element Pump Sampler Column Compartment Part Number G7120A G7112B G7111B G7104A G7104C G4220A/B G4204A G1312B G7129B G7167B G7116B G1316C G7117A/B/C G7114A/B Detector G7115A G7165A Module Comment 1290 Infinity II High Speed Pump 1260 Infinity II Binary Pump 1290 Infinity II Quaternary Pump 1290 Infinity II Flexible Pump 1260 Infinity II Flexible Pump 1290 Infinity Binary Pump 1290 Infinity Quarternary Pump 1260 Infinity Binary Pump 1290 Infinity II Vialsampler 1290 Infinity II Multisampler 1290 Infinity II Multicolumn Thermostat 1290 Infinity Thermostatted Column Compartment 1260/1290 Infinity II Diode Array Detector 1260/1290 Infinity II Variable Wavelength Detector 1260 Infinity II Diode Array Detector WR 1260 Infinity II Multiple Wavelength Detector Recommended for multiple heart-cutting and high-resolution sampling as a peak trigger or for monitoring. Optional for comprehensive 2D-LC. 1D flow cells require a minimum pressure stability of 60 bar (which excludes FLD and RID detectors). 3 Options Table 1 Overview of recommended hardware configurations Function in 2D Interface 2nd dimension Functional Element Valve drive Part Number G1170A 2D-LC Valve G4236A G4243A MHC Valves G4236A#007 G4243A#007 G4242A Pressure Relief G4236-60010 Kit (PRK) Pump Column Compartment G7120A G4220A/B G7116B G1316C Detector G7117A/B/C G7114A/B G7115A G7165A G1321B G4260A Module Comment 1290 Infinity Valve Drive 1x for SHC, LCxLC 3x for MHC, HighRes Sampling 2D-LC valve kit, Standard Contains the 2D-LC valve head 2D-LC valve kit, ASM Contains the 2D-LC valve head with Active Solvent Modulation (ASM) functionality Multiple Heart-Cutting Kit Contains two MHC valve heads 2D-LC Multiple Heart-Cutting Upgrade Kit Kit to upgrade MHC valves to an existing 2D-LC system Pressure Relief Kit Mandatory if a 1D detector is used. The kit prevents pressure pulses and protects detector flow cells! 1290 Infinity II High Speed Pump Infinity 1290 Binary Pump 1290 Infinity or Infinity II Binary Pump required. 1290 Infinity II Multicolumn Thermostat 1290 Infinity Thermostatted Column Compartment Optional: A second column compartment is optional for large temperature differences between 1st and 2nd dimension. Any of these are supported as well as others or older modules. 1260/1290 Infinity II Diode Array Detector 1260/1290 Infinity II Variable Wavelength Detector 1260 Infinity II Diode Array Detector WR 1260 Infinity II Multiple Wavelength Detector 1260 Infinity FLD 1260 Infinity ELSD 4 Recommendations for instrument setup General Information Recommendations for instrument setup General Information InfinityLab 2D-LC Solutions come in several flavors, still allowing flexible combination of InfinityLab Series and 1200 Series Infinity modules. For 2D-LC, a two-stack configuration is necessary. On the left stack, the order of the modules from bottom to top is: pumps for both dimensions, then Vial- or Multisampler. The sampler must be placed on top of the pumps. The right stack consists of one or two column compartments and one or two detectors. When using multiple detectors at different positions of the system, it is recommended to use the same detector type. Depending on the number of solvents used, both stacks offer the possibility to place a solvent cabinet on top. NOTE Figure 1 Left: Recommended stack configuration for the 1290 Infinity II 2D-LC System. Right: Bench space requirements of the 1290 Infinity II 2D-LC System. The dual stack configuration for 2D-LC requires at least 97 x 62 cm (24.4 x 38.2 inches) free, vertical bench space. 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear is reserved for air circulation and electric connections. Agilent InfinityLab 2D-LC Solutions offer two general valve configurations that decide that decide which of the 2D-LC modes that can be used with the instrument. While the Single Heart-Cutting (SHC) configuration offers access to Single Heart-Cutting and Comprehensive 2D-LC, the Multiple Heart-Cutting (MHC) configurations additionally gives access to Multiple Heart-Cutting and High-Resolution Sampling 2D-LC. In addition, the Active Solvent Modulation valve (G4243A) is only available for the MHC configuration. An overview of all available 2D-LC modes can be found in Optional hardware configurations (Table 1 on page 3). Stack setups of all other LC modules (reference) remain valid since those setups are independent of the valve configuration. 5 Recommendations for instrument setup General Information Table 2 Overview of 2D-LC modes dependent on valve configuration of the 2D-LC system Valves SHC Configuration MHC Configuration 2D-LC Valve, Standard 2D-LC Valve, Active Solvent Modulation (ASM) Operation Modes Comprehensive (LCxLC) Single Heart-Cutting Multiple Heart-Cutting High Resolution Sampling X SHC Configuration X X MHC Configuration 6 Recommendations for instrument setup Single Heart-Cutting Configuration Single Heart-Cutting Configuration 2D-LC instruments that are exclusively used for Single Heart-Cutting and Comprehensive 2D-LC experiments only require the standard 2D-LC valve (G4236A). The valve can be conveniently attached to any Infinity II pump that is installed. For a SHC configuration, transfer capillaries (6a/6b) are not necessary since MHC decks are not installed. Figure 2 Schematics of a Single Heart-Cutting (SHC) Configuration with supported valves. For technical reasons, the ASM valve (G4243A) is not supported in Single Heart-Cutting setups. Multiple Heart-Cutting Configuration 2D-LC instruments that are used for Multiple Heart-Cutting or High-Resolution Sampling 2D-LC require additional MHC decks. For MHC configurations, both the standard 2D-LC valve (G4236A) and the ASM valve head (G4243A) are supported. The valves can be conveniently attached to any Infinity II pump in the stack. Depending on the valve head that is used, different transfer capillaries (6a/6b) must be installed. For installation, please follow the guidance below. Figure 3 Schematics of a Multiple Heart-Cutting (MHC) Configuration with supported valves and transfer capillaries. 7 Recommendations for instrument setup Recommended stack setups Recommended stack setups InfinityLab 2D-LC Solutions allow three basic stack setups in three variations depending on the column compartment concept that is used. The basic stack configurations are distinguished by the pump used for the first and second dimension. In the second dimension a 1290 Infinity or 1290 Infinity II High-Speed Pump is mandatory. Agilent 1290 Infinity pumps are always based on the bottom. The capillary kit covers all recommended configurations. The following configurations optimize the system flow path, ensuring minimum delay and dispersion volumes: Table 3 Supported instrument configurations with a list of supported LC pumps. Numbers refer to the stack setup that is recommended. # 1D pump supported 2D pumps 1 1290 Infinity II / 1260 Infinity II Prime LC 1290 Infinity / 1290 Infinity II 1260 Infinity II Flexible Pump (G7104C) 1290 Infinity II High-Speed Pump (G7120A) 1290 Infinity II Flexible Pump (G7104A) 1290 Infinity Binary Pump (G4220A) 1290 Infinity II High-Speed Pump (G7120A) 2 1290 Infinity 1290 Infinity II 1290 Infinity Quaternary Pump (G4204A) 1290 Infinity II High-Speed Pump (G7120A) 1290 Infinity Binary Pump (G4220A) 3 1260 Infinity Binary / 1260 Infinity II Binary 1290 Infinity II 1260 Infinity II Binary Pump (G7112B) 1290 Infinity II High-Speed Pump (G7120A) 1260 Infinity Binary Pump (G1312B) NOTE This guide only covers setups that contain at least one Infinity II pump module! Setups that contain exclusively 1200 Infinity Series modules must be installed with the corresponding capillary kit. Connections mentioned in this setup are con-current direction for the Standard 2D-LC Valve (G4236A) and countercurrent for the ASM 2D-LC Valve (G4243A). To connect the 2D-LC Valve, Standard in countercurrent direction, please follow the schematics shown in the 2D-LC Software Configuration screen. For more information, please read the 2D-LC Software Configuration section below. Connection to valve port are mentioned in brackets, for example. ASM Valve (2) = ASM Valve, Port 2. 8 Recommendations for instrument setup Recommended stack setups Figure 4 Stack Setup #1. Recommended setup if both pumps are Infinity II modules or the 2D pump is a 1290 Infinity Binary pump. Number of Capillary 1a 2 3a 4 5 6a 6b 7 8 9 10 11 # Connection L x ID [mm] P/N 1 1D pump (top) to autosampler 400 x 0.17 5500-1245 1 Autosampler to 1D column (in MCT) 600 x 0.12 5067-4669 1 1D column to 1D DAD 400 x 0.12 5500-1251 1 1D DAD to T-piece of PRK 400 x 0.17 5500-1245 1 T-piece of PRK to Standard 2D-LC Valve (Port 105 x 0.17 3) / ASM Valve (Port 4) 5500-1240 4 2D-LC Valve (1) - Deck (IN) Deck (Out) - 170 x 0.12 2D-LC Valve (8) 2D-LC Valve (5) - Deck (IN) Deck (Out) - 2D-LC Valve (4) 5500-1270 4 ASM Valve (7) - Deck (IN) Deck (Out) - 170 x 0.12 ASM Valve (3) ASM Valve (2) - Deck (IN) Deck (Out) - ASM Valve (8) 5500-1376 1 2D-LC valve (6) / ASM valve (10) to 2D column (in MCT) 400 x 0.12 5500-1251 1 2D column (in MCT) to 2D DAD 280 x 0.12 5067-4651 1 2D pump to 2D-LC Valve (7) / ASM Valve (5) 280 x 0.17 5067-4608 1 T-piece of PRK to damper capillary 150 x 0.17 5500-1227 1 waste line self-cut x 0.7 0890-1713 Description Capillary ST 0.17x400 SI/SI Capillary ST 0.12x600 S/SL Capillary ST 0.12x400 SL/SL Capillary ST 0.17x400 SI/SI Capillary ST 0.17x105 SL/SL Capillary ST 0.12x170 S/M Capillary ST 0.12x170 M/M (delivered with 2D-LC Valve Kit, ASM) Capillary ST 0.12x400 SL/SL Capillary ST 0.12x280 SL/SX Capillary ST 0.17x280 SX/S Capillary ST 0.17x150 SL/SL Tubing-flexible 0.8/1.61mm PTFE WT (delivered with UV detector) 9 Recommendations for instrument setup Recommended stack setups Figure 5 Stack Setup #2. Recommended setup if the 1D pump is a 1290 Infinity Binary Pump or a 1290 Infinity Quaternary Pump. Number of Capillary 1b 2 3a 4 5 6a 6b 7 8 9 10 11 # Connection L x ID [mm] P/N 1 1D pump (bottom) to sampler 600 x 0.17 5067-4670 1 Autosampler to 1D column (in MCT) 600 x 0.12 5067-4669 1 1D column to 1D DAD 400 x 0.12 5500-1251 1 1D DAD to T-piece of PRK 400 x 0.17 5500-1245 1 T-piece of PRK to Standard 2D-LC Valve (Port 105 x 0.17 3) / ASM Valve (Port 4) 5500-1240 4 2D-LC Valve (1) - Deck (IN) Deck (Out) - 170 x 0.12 2D-LC Valve (8) 2D-LC Valve (5) - Deck (IN) Deck (Out) - 2D-LC Valve (4) 5500-1270 4 ASM Valve (7) - Deck (IN) Deck (Out) - 170 x 0.12 ASM Valve (3) ASM Valve (2) - Deck (IN) Deck (Out) - ASM Valve (8) 5500-1376 1 2D-LC valve (6) / ASM valve (10) to 2D column (in MCT) 400 x 0.12 5500-1251 1 2D column (in MCT) to 2D DAD 280 x 0.12 5067-4651 1 2D pump to 2D-LC Valve (7) / ASM Valve (5) 280 x 0.17 5067-4608 1 T-piece of PRK to damper capillary 150 x 0.17 5500-1227 1 waste line self-cut x 0.7 0890-1713 Description Capillary ST 0.17x600 S/SH Capillary ST 0.12x600 S/SL Capillary ST 0.12x400 SL/SL Capillary ST 0.17x400 SI/SI Capillary ST 0.17x105 SL/SL Capillary ST 0.12x170 S/M Capillary ST 0.12x170 M/M (delivered with 2D-LC Valve Kit, ASM) Capillary ST 0.12x400 SL/SL Capillary ST 0.12x280 SL/SX Capillary ST 0.17x280 SX/S Capillary ST 0.17x150 SL/SL Tubing-flexible 0.8/1.61mm PTFE WT (delivered with UV detector) 10 Recommendations for instrument setup Recommended stack setups Figure 6 Stack Setup #3. Recommended setup if the 1D pump is a 1260 Infinity or 1260 Infinity II Binary Pump. Number of Capillary 1c 2 3a 4 5 6a 6b 7 8 9 10 11 # Connection L x ID [mm] P/N 1 1D pump (bottom) to sampler 900 x 0.17 5500-1217 1 Autosampler to 1D column (in MCT) 600 x 0.12 5067-4669 1 1D column to 1D DAD 400 x 0.12 5500-1251 1 1D DAD to T-piece of PRK 400 x 0.17 5500-1245 1 T-piece of PRK to Standard 2D-LC Valve (Port 105 x 0.17 3) / ASM Valve (Port 4) 5500-1240 4 2D-LC Valve (1) - Deck (IN) Deck (Out) - 170 x 0.12 2D-LC Valve (8) 2D-LC Valve (5) - Deck (IN) Deck (Out) - 2D-LC Valve (4) 5500-1270 4 ASM Valve (7) - Deck (IN) Deck (Out) - 170 x 0.12 ASM Valve (3) ASM Valve (2) - Deck (IN) Deck (Out) - ASM Valve (8) 5500-1376 1 2D-LC valve (6) / ASM valve (10) to 2D column (in MCT) 400 x 0.12 5500-1251 1 2D column (in MCT) to 2D DAD 280 x 0.12 5067-4651 1 2D pump to 2D-LC Valve (7) / ASM Valve (5) 280 x 0.17 5067-4608 1 T-piece of PRK to damper capillary 150 x 0.17 5500-1227 1 waste line self-cut x 0.7 0890-1713 Description Capillary ST 0.17x900 SI/SX Capillary ST 0.12x600 S/SL Capillary ST 0.12x400 SL/SL Capillary ST 0.17x400 SI/SI Capillary ST 0.17x105 SL/SL Capillary ST 0.12x170 S/M Capillary ST 0.12x170 M/M (delivered with 2D-LC Valve Kit, ASM) Capillary ST 0.12x400 SL/SL Capillary ST 0.12x280 SL/SX Capillary ST 0.17x280 SX/S Capillary ST 0.17x150 SL/SL Tubing-flexible 0.8/1.61mm PTFE WT (delivered with UV detector) 11 Recommendations for instrument setup Alternative instrument setups for additional functionality Alternative instrument setups for additional functionality The standard stack setups can be upgraded with additional valves to add additional functionality. Table 2 gives an overview of all supported modifications of a standard 2D-LC instrument. At a time, only one modification is recommended to ensure correct operation of the instrument. The standard stack setup uses one column compartment that hosts both the 1D and 2D column. Table 4 List up supported modifications of a standard 2D-LC instrument configuration. Alternative column compartment concepts Comment Page A 1D MCT/TCC hosts column switching valve If a 6-position/14-port or 8-position/18-port InfinityLab Quick Change Valve is used, additional two adapters necessary (2xG1316-87326, must be purchased separately) See Figure 7 on page 13 B Setups that contain separate 1D and 2D MCTs/TCCs See Figure 8 on page 14 C Setups in which the 1D column Longer capillary (5500-1170) for Quick Connect Fitting at column See Figure 9 on page 15 is hosted in an Integrated inlet or new 0.12x280mm Quick Connect Fitting assembly Column Compartment (ICC) (5067-5960) necessary (must be purchased separately). D Setup with a MS diverter valve See Figure 10 on page 16 E Setup of a 1D/2D Switching Valve If a 1D and 2D detector is used; not supported with modifications See Figure 11 on page 17 A-C F 1D/2D Switching Valve w/o 1D For setups that do not have a 1D detector, e.g. for certain LCxLC See Figure 12 on page 18 detector setups or setups with a QQQ mass spectrometer as a 2D detector; not supported with modifications A-C 12 Recommendations for instrument setup Alternative instrument setups for additional functionality Figure 7 Setup A. Recommended setup if a column switching valve (for example 6-position/14-port InfinityLab Quick-Change Valve) is used. For a InfinityLab 2-position/6-port Quick-Change Valve, adapters A1 are not necessary. Number of # Capillary Connection L x ID [mm] P/N Description A1 2 Adapter: capillary 2 to column switching 75x0.12mm G1316-87326 SST Capillary 75x0.12mm, valve, (Port IN) / Adapter column f/m, ns 0.8 (must be switching valve (Port OUT) to capillary 3a purchased separately) For all other capillaries / connections, please refer to Figure 4 on page 9, Figure 5 on page 10, and Figure 6 on page 11. NOTE Adapters to and from the column switching valve are only necessary if a 6-position/14-port InfinityLab Quick-Change Valve or a for example 8-position/18-port InfinityLab Quick-Change Valve is used. 13 Recommendations for instrument setup Alternative instrument setups for additional functionality Figure 8 Setup B. Recommended setup if the instrument contains separate MCTs/ TCCs for 1D and 2D columns. Number of # Capillary Connection L x ID [mm] P/N Description 3b 1 1D column to 1D DAD 280 x 0.12 5067-4651 Capillary ST 0.12x280 SL/SX 8 1 2D column (in 2D MCT) to 2D DAD 280 x 0.12 5067-4651 Capillary ST 0.12x280 SL/SX (part of 2D-LC capillary kit) For all other capillaries / connections, please refer to Figure 4 on page 9, Figure 5 on page 10, and Figure 6 on page 11. 14 Recommendations for instrument setup Alternative instrument setups for additional functionality Figure 9 Setup C. Recommended setup if 1D column is hosted in an Integrated Column Compartment (ICC). Number of # Capillary Connection L x ID [mm] P/N Description C1 1 Injection Valve to ICC 0.12x105mm 5500-1238 Capillary ST 0.12x105 SL/SL (provided with ICC) C2 1 Heat exchanger out to column (InfinityLab 0.12x280mm 5500-1170 Capillary ST 0.12x280 (must Quick Connect Fitting) be purchased separately) 8 1 2D column (in 2D MCT) to 2D DAD 280 x 0.12 5067-4651 Capillary ST 0.12x280 SL/SX (part of 2D-LC capillary kit) For all other capillaries / connections, please refer to Figure 4 on page 9, Figure 5 on page 10, and Figure 6 on page 11. The 2D-LC Software allows only certain valves to be configured as diverter valves. A list of supported valves can be found in Table 3 on page 8. More information is available in the Agilent Technical Note Agilent InfinityLab 2D-LC Solution with mass spectrometric detection and diverter valve (G4236-90100). p/n G4231A G4231C G4232C G4232D Description 2pos/6port valve head, 800 bar 2pos/6port valve head, 1300 bar 2pos/10port valve head, 800 bar 2pos/10port valve head, 1300 bar 15 Recommendations for instrument setup Alternative instrument setups for additional functionality Figure 10 Setup D. Recommended setup of a MS diverter valve. Number of # Capillary Connection L x ID [mm] P/N Description D1 1 Capillary from 2D detector to T-piece 0.12 x 400 5067-4606 Capillary ST 400x0.12 S/SH D2 1 T-piece 0100-0969 1/16in Tee, SST, Low Dead Volume D3 1 Capillary from MS to T-piece (self cut) 0.12 x 400 0890-1915 Capillary PEEK, 0.12x1250 D4 2 T-piece to pressure relief valve; pressure 0.3 x 80 5500-1228 Capillary ST 0.3x80 SL/SL relief valve to diverter valve D5 1 Pressure relief valve G4212-60022 Pressure relief valve D6 1 blank nut 01080-83202 Blanking Nut 1/16 in SST D7 1 diverter valve to waste 5062-2462 Tubing PTFE 0.7 mm x 5m D8 1 peak fittings 5063-6591 Fitting-Fingertight PEEK for 1/16-in D9 1 Valve holder for Valve drive to attach to MCT 5067-6138 Valve Holder Kit Right-IF-II-G D10 1 Diverter Valve G4231A G4231C G4232A G4232C 2pos/6port, 800bar 2pos/6port, 1300bar 2pos/10port, 800bar 2pos/10port, 1300bar For all other capillaries / connections, please refer to Figure 4 on page 9, Figure 5 on page 10, and Figure 6 on page 11. 16 Recommendations for instrument setup Alternative instrument setups for additional functionality The 1D/2D switching valve offers the possibility to exclude the 2D flow path of the instrument to run both 1D and 2D experiments which is useful for example if one mass spectrometer is used for both 1D and 2D experiments. Two basic setups are supported (setup E and F). The recommended setups for a 1D/2D Switching valves do not support the use of ICC column compartments, column switching valves or the use of separate 1D and 2D MCTs/TCCs! To run 1D experiments, the 2D mode must be disabled in the 2D-LC Software Configuration Tab. Figure 11 Setup E. Recommended setup for the 1D/2D switching valve. Number of # Capillary Connection L x ID [mm] P/N Description 3a 1 MCT / TCC to 1D DAD 400 x 0.12 5500-1251 Capillary ST 0.12x400 SL/SL 8 1 1D MCT / TCC to 1D DAD 280 x 0.12 5067-4651 Capillary ST 0.12x280 SL/SX E1 2 1D column to 1D/2D Switching Valve (1); 120 x 0.12 5067-4652 Capillary ST 0.12x 120 SX/SX 2D column to 1D/2D Switching Valve (3) E2 1 Connection capillary 1D/2D Switching 90 x 0.12 5067-4649 Capillary ST 0.12x90 SX/S Valve (4) to (5) For all other capillaries / connections, please refer to Figure 4 on page 9, Figure 5 on page 10, and Figure 6 on page 11. 17 Recommendations for instrument setup Alternative instrument setups for additional functionality Figure 12 Setup F. Recommended setup for the 1D/2D switching valve without 1D detector. Number of # Capillary Connection L x ID [mm] P/N Description 3b 1 1D/2D Switching Valve (2) to 2D DAD 280 x 0.12 5067-4651 Capillary ST 0.12x280 SL/SX F1 2 1D column to 1D/2D Switching Valve (1); 120 x 0.12 5067-4652 Capillary ST 0.12x 120 SX/SX 2D column to 1D/2D switching valve (3) F2 1 Connection 1D/2D switching valve ports 90 x 0.12 5067-4649 Capillary ST 0.12x90 SX/S (4) to (5) F3 1 MCT/TCC to 2D-LC valve (6) / ASM valve 500 x 0.12 5500-1157 Capillary ST 0.12x500 SL/S (4) For all other capillaries / connections, please refer to Figure 4 on page 9, Figure 5 on page 10, and Figure 6 on page 11. 18 Installation of the 2D-Valves Attaching the external valve drives Installation of the 2D-Valves Attaching the external valve drives For InfinityLab 2D-LC instruments that comprise at least one 1260 Infinity II or 1290 Infinity II pump, valve drives are attached to this pump with the Valve Clamp Kit IF II (5067-5685), while the valve drives are interconnected by the Adapter profile (5043-0269). The 2D-LC valve and if selected the MHC decks are mounted on external valve drives (G1170A). # Holders / connectors Connection P/N 3 1290 Infinity Valve Drive (must be purchased separately) Mooting of Valves G1170A 1 Clamp Guide Kit IF II (delivered with G1170A) Top valve to pump 5067-5685 2 Adapter-profile (delivered with MHC Decks) between G1170A drives 5043-0269 For a SHC configuration, the 2D-LC valve (G4236A) is attached to the upper pump of the stack. In case of a MHC configuration, the upper MHC deck is attached to the upper pump. Figure 13 Schematic of the installation and attachments of the 2D-LC valve and optionally the MHC decks. 1 Mount the clamp guide on the right side of the Infinity II Pump: Markings in the form of round dips are on the body housing. Make a small hole with a peaked screw driver and tighten the clamp guide with the 3 self-cutting tapping screws. 2 Mount the valve heads on the G1170A external valve drives. 3 Clamp the first external valve drive with the MHC valve on top. 4 Attach the adapter-profile on each of the other external valve drives and mount them according to the positions shown in Figure 13 on page 19. 5 Mount the leak tray with sensor underneath the lowest external valve drive. 6 Install the Pressure release kit, see "Installing the Pressure Release Kit" on page 24. 19 Installation of the 2D-Valves Connecting the 2D-LC Valve, Standard (G4236A) Connecting the 2D-LC Valve, Standard (G4236A) The capillary connections of the 2D-LC valves depend on whether a con- or countercurrent configuration achieved. For the standard 2D-LC Valve, both concurrent and countercurrent operation is possible. Schematics in this chapter will reflect a concurrent direction. For the countercurrent setup, please refer to Countercurrent configuration of the Standard 2D-LC Valve in the 2D-LC Software. You find the "Valve topology" configuration screen in OpenLab CDS ChemStation Edition under Instrument>2D-LC Configuration. Figure 14 Schematic representation of the Standard 2D-LC Valve (G4236A) in concurrent flow. 20 Installation of the 2D-Valves Connecting the 2D-LC Valve, Standard (G4236A) Port Number of Connection Capillary L x ID [mm] P/N Description 1 6a transfer capillary to MHC Valve (OUT), deck A 170 x 0.12 5500-1270 Capillary ST 0.12x170 S/M 2 11 waste line self-cut x 0.7 0890-1713 Tubing-flexible 0.8/1.61mm PTFE WT (delivered with UV detector) 3 5 F3 from pressure release kit; from 1D column, 1D 105 x 0.17 500 5500-1240 Capillary ST 0.17x105 SL/SL detector x 0.12 5500-1157 Capillary ST 0.12x500 SL/S 4 6a transfer capillary to MHC Valve (IN), deck B 170 x 0.12 5500-1270 Capillary ST 0.12x170 S/M 5 6a transfer capillary to MHC Valve (OUT), deck B 170 x 0.12 5500-1270 Capillary ST 0.12x170 S/M 6 7 to 2D column 400 x 0.12 5500-1251 Capillary ST 0.12x400 SL/SL 7 9 from 2D pump 280 x 0.17 5067-4608 Capillary ST 0.17x280 SX/S 8 6a transfer capillary to MHC Valve (IN), deck A 170 x 0.12 5500-1270 Capillary ST 0.12x170 S/M 21 Installation of the 2D-Valves Connecting the 2D-LC Valve, ASM (G4243A) Connecting the 2D-LC Valve, ASM (G4243A) In contrast to the standard 2D-LC Valve (G4236A) Agilent recommends using a counter-current configuration for the ASM 2D-LC Valve (G4243A) when working in ASM mode. This section describes the setup for a counter-current configuration of the ASM Valve. For the concurrent setup, please refer to concurrent configuration of the ASM 2D-LC Valve in the 2D-LC Software. You find the Valve topology configuration screen in OpenLab CDS ChemStation Edition under Instrument > 2D-LC Configuration. The installation of a 2D-LC system depends on which modules you are using for which 2D-LC mode and is described above. The connection scheme is displayed in the graphical user interface of the 2D-LC Configuration as Valve Topology: Figure 15 Schematic representation of the ASM 2D-LC Valve (G4243A) in countercurrent flow. 22 Installation of the 2D-Valves Connecting the 2D-LC Valve, ASM (G4243A) Port Number of Connection Capillary L x ID [mm] P/N Description 1 11 waste line self-cut x 0.7 0890-1713 Tubing-flexible 0.8/1.61mm PTFE WT (delivered with UV detector) 2 6b transfer capillary to MHC Valve (IN), deck A 170 x 0.12 5500-1376 Capillary ST 0.12x170 M/M 3 6b transfer capillary from MHC Valve (OUT), deck 170 x 0.12 B 5500-1376 Capillary ST 0.12x170 M/M 4 5 F3 from pressure release kit; from 1D column, 1D 105 x 0.17 500 5500-1240 Capillary ST 0.17x105 SL/SL detector x 0.12 5500-1157 Capillary ST 0.12x500 SL/S 5 9 from 2D pump 280 x 0.17 5067-4608 Capillary ST 0.17x280 SX/S 6 ASM1-4 outlet to ASM capillary L x 0.12 see list below 7 6b transfer capillary to MHC Valve (IN), deck B 170 x 0.12 5500-1376 Capillary ST 0.12x170 M/M 8 6b transfer capillary from MHC Valve (OUT), deck 170 x 0.12 A 5500-1376 Capillary ST 0.12x170 M/M 9 ASM1-4 inlet from ASM capillary L x 0.12 see list below 10 7 to 2D column 400 x 0.12 5500-1251 Capillary ST 0.12x400 SL/SL Which ASM capillary shall be used depends on the ASM factor, which is optimum for your application. You may choose from following capillaries: Capillary p/n 5500-1300 Length (mm) 85 Inner diameter (mm) 0.12 Volume (µl) 0.96 ASM factor 5 Split ratio (loop:ASM) 1:4 5500-1301 170 0.12 1.9 3 1:2 5500-1302 340 0.12 3.8 2 1:1 5500-1303 680 0.12 7.7 1.5 1:0.5 23 Installation of the 2D-Valves Installing the Pressure Release Kit Installing the Pressure Release Kit )URP'$'FHOORXW &DSLOODU\67[6/6/ &DSLOODU\ 67[6,6, &DSLOODU\67[6/6/ 7R'/&YDOYH9DOYHSRUW Figure 16 Connections to the pressure release kit 7RGDPSHUFDSLOODU\ Parts required # p/n 1 G4236-60010 Description 2D-LC Pressure Release Kit 1 Push the pressure release valve assembly in the frame. 2 Take care for the correct orientation. 7SLHFH LQOLQHZLWKWKHGHWHFWRU WRZDVWH 24 Installation of the 2D-Valves Installing the Pressure Release Kit 3 Insert the pressure release assembly to the leak tray, orientation as shown. WRZDVWH 4 Push the pressure release assembly in the correct position. 7SLHFHLQOLQHZLWKWKHGHWHFWRU 25 2D-LC Software Configuration Installing the Pressure Release Kit 2D-LC Software Configuration All 2D-LC specific configurations are defined in the "Configure 2D-LC..." screen. This screen allows you to: · Select your 1D and 2D pump · Valve topology · 2D-LC valve head (if multiple Valve heads are available) · Select the connections of MHC decks (if installed) · Capillary connections 26 2D-LC Software Configuration Installing the Pressure Release Kit Figure 17 The Configure 2D-LC screen. All basic configurations of the 2D-LC systems, viz. 1D and 2D pump, valve topology, capillary connections as well as all 2D-LC valves are defined here. 1 Pump configuration 2 Valve topology 3 2D-LC Valve 4 Multiple Heart-Cutting Valves 5 Capillaries 27 2D-LC Software Configuration Installing the Pressure Release Kit 1 In OpenLab ChemStation under Method and Run Control, click on Instrument, then 2D-LC Configuration... 2 Select your your 1D and your 2D pump. Please note that this will not rename your pumps. A descriptive naming should be also entered during initial instrument setup in the instrument configuration. 3 Select your valve topology. Depending on the 2D-LC valve that you have installed, viz. the Standard 2D-LC (G4236A) or the ASM 2D-LC Valve (G4243A), your valve will automatically appear here. Please specify whether you connected your valve in concurrent or countercurrent mode 4 Optional: if the system contains multiple 2D-LC valves, please specify which valve head is to be used. 5 Optional: if your system contains Multiple Heart-Cutting decks, specify which valve head is corresponds to Deck A or B respectively. 6 Select your installed capillaries. Check for correct loop size and correct length of the transfer capillaries. If an ASM 2D-LC valve is used, define the ASM capillary which defines your split ratio (see Table on page 23). a To define a ASM capillary, click on Capillaries.... b Choose a pre-defined ASM capillary OR Define a generic capillary by choosing Generic Capillary. Figure 18 The Setup Capillaries screen. Here, you can define ASM and transfer capillaries as well as define other loop sizes. The settings for the transfer volumes are 13.4 L between 1D detector and sample loop, and 1.9 L between 2D-LC valve and MHC valve for a set-up with a MaxLight Cartridge Cell. For additional information, please refer to the 2D-LC Solution Manual (G2198-90501). *G4236-90021* *G4236-90021* G4236-90021 Edition: 11/2018 Printed in Germany © Agilent Technologies, Inc 2016-2018 Agilent Technologies, Inc Hewlett-Packard-Strasse 8 76337 Waldbronn GermanyAcrobat Distiller 11.0 (Windows)