Supplementary File 2 JME AV7.1 239 2009 9258 MOESM4 ESM
User Manual: AV7.1
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Av7-1 Av7-2 Av7-3 Av10 TTGTGAAGCCAAGAAGGAAACATCAACAAAGAAAACAGTCAAATTTTGATTCAA 54 ****************************************************** 54 ----------------------------------------------------------------------------------------------------------- Av7-1 Av7-2 Av7-3 Av10 GTAAGTTTGATGGATGTCATTTCTTCTTACGATTTTATTTAAAGAATATATACT 108 ****************************************************-* 107 ----------------------------------------------------------------------------------------------------------- Av7-1 Av7-2 Av7-3 Av10 GTATTTATACTCATTATTATATCATGATTCCCATCAAACCGACCCTTTTCCTTT 162 *********-******************************************** 160 ----------------------------------------------------------------------------------------------------------- Av7-1 Av7-2 Av7-3 Av10 CGAAGCAACAGGCGGGTTAGGACTGTACTAAACCCTCCTGAGTTATAATTATAA 216 ****************************************************** 214 ----------------------------------------------------------------------------------------------------------- Av7-1 Av7-2 Av7-3 Av10 AATTAAGAGATTTTATTTCTGTTTGTTTTCCTACAATCTAATCAAACCAAACAC 270 ****************************************************** 268 ----------------------------------------------------------------------------------------------------------- Av7-1 Av7-2 Av7-3 Av10 ATCAGGAGTGACCAAGAGCGACTTTTGTTTATCAGTATGTTCTTTTCTTTATTT 324 *****************************************-************ 321 ----------------------------------------------------------------------------------------------------------- Av7-1 Av7-2 Av7-3 Av10 CCACGCTTACCCTCAAGGGCTGGGTATGTCCACAGAACAGAACTTCTCCACTAC 378 ****************************************************** 375 ----------------------------------------------------------------------------------------------------------- Av7-1 Av7-2 Av7-3 Av10 GGGTAGCACTAGCACTTTTTTATCACTAAAGGAGCATTTTCAATGAATAAGCGA 432 ****************************************************A* 429 ----------------------------------------------------------------------------------------------------------- Av7-1 Av7-2 Av7-3 Av10 Av7-1 AATATCTTTATGATGTCACCATTTTACTGTTGGCCATCTAGAATGGCATCTCTC 486 ****************************************************** 483 ----------------------------------------------------------------------------------------------------------TCTGGACGTGTCTTTTGTAAAAAAAAATAAAAAAATTTAGCCGCATGCGTCTAC 540 Av7-2 ****************************************************** 537 Av7-3 -----------------------------------------------------Av10 -----------------------------------------------------Av7-1 Av7-2 Av7-3 Av10 TTTTTGTAATCTGTTAAATACACAAAAAATGGTTCATACATTCTTTTTTAACAG 594 ****************************-************************* 591 ----------------------------------------------------------------------------------------------------------- [M] M N R L L V F L M L G A/- A F Av7-1 AAAGCTACAGTGATGAACCGTCTTTTGGTCTTTCTGATGCTCGGGGCAGCTTTT Av7-2 ****************************************************** Av7-3 ---------A**************C********************---*****C Av10 ---------A**************C********************---*****C 648 645 42 42 Av7 S1D (part 1) Av7-1 Av7-2 Av7-3 Av10 M L V V S A N D A Y G ATGCTTGTCGTTTCAGCCAATGATGCTTATGGCGGTAAGATTCTAAATGGGGACA ******************************************************* *******************************************G*********** *******************************************G*********** Av7-1 Av7-2 Av7-3 Av10 TAGAGAGAGTAGAAGAGAGGGAGAGAGAGGGAGTTTGAGAGA-----------*********************************AG*TT****-----------*********************************AGA******----AATTGAGT *********************************AGA******GAGAAATTGAGT 744 741 146 150 Av7-1 Av7-2 Av7-3 Av10 --------------GAGAGAGGCAAGTTGTGTACTCACAAATATGTGCACAATT ------------GA*******************TC*-***************** TAGGGAGATTGAGA********************C*-***************** TAGGGAGATTGAGA********************C*-***************** 784 782 199 203 Av7-1 Av7-2 Av7-3 Av10 GTTCGTCGCTATATCGGATCTTTATCGGATAATTCATGCTGAAATTAATGCCAG ************************************G*********-------************************************G*********GT***--************************************G*********GT***--- 838 828 250 254 Av7-1 Av7-2 Av7-3 Av10 TAATAGATGGATACAAAGCTATCTTGACCAGAAATGTGGAACACAACTCAACTC -----------------------------------------------------------*C**G**A*GCAAC*CT**T*GATT*T**AA*A**G***CTA*T**-------*C**G**A*GCAAC*CT**T*GATT*T**AA*A**G***CTA*T**-- 892 828 296 300 Av7-1 Av7-2 Av7-3 Av10 ATGAATAATGTTACGATTCTCTTGTTAAAATTCTTTTTTCTGAGCAAACTTTTT ---------------------------------------------------------------------------------------------------------------------------------------------------------------- 946 828 296 300 703 700 97 97 Av7-1 Av7-2 Av7-3 Av10 CATGTTCAGTTGGTTAGTGTGCGTCCTTCGTAGATGAAGGCCCTCGGTTCGACT ---------------------------------------------------------------------------------------------------------------------------------------------------------------- 1000 828 296 300 Av7-1 Av7-2 Av7-3 Av10 CCCAGGATCCACAAATCTATCTGCTTTGACTTCTCTTTTTCCGTGTAGCTACAG ----------------------------------------------------------------A*GT*G****C**GCGTGTC*A*T**CC*T*T**C*TAC***A -----------A*GT*G****C**GCGTGTC*T*T**CC*T*T**C*TAC***A 1054 828 339 343 Av7-1 Av7-2 Av7-3 Av10 CTTAAATAACTTTGTAAGGAGCACTGATGGTTTGAGGGGGTCAGTAAAGTGCTC -----------------------------------------------------G***C*ATT****C*G--------------------------------*CC*** G***C*ATT****C*G--------------------------------*CC*** 1108 828 361 365 Av7-1 Av7-2 Av7-3 Av10 ACCTTCGATTGC-TTAAACTATTTGGTAATCAACCTCTCCTTTACCTATGCCTT -----------------------------------------------------G********CATG***C*****-------------------------------G********CATG***C*****-------------------------------- 1161 828 383 387 Av7-1 Av7-2 Av7-3 Av10 TTTTTTCAGACGAGCCGGCATTTAAAGACCTCAAACGAGGTATCGTATAAAGTG -------------------------------------------------------------------------------***TCT*GGA*G**AGGGG*GCCCC**A --------------------------***TCT*GGA*G**AGGGG*GCCCC**A 1215 828 411 415 Av7-1 Av7-2 Av7-3 Av10 TTTAAATTATCGAATTTTAGTGGAAACTGACAATGTGAAAATGATATATTCAAA -----------------------------------------------------C*A*C*A*G*-----------------------**CA*****ATCC*G*C**C* C*A*C*A*G*-----------------------**CA*****ATCC*G*C**C* 1269 828 442 446 Av7-1 Av7-2 Av7-3 Av10 GAACAAAT-CCATTAATATAATGTCATTCAGGGGAAGAAAACTGAAGGA-ATTA -----***G***G*****G*T----------**ATG**C****C*T*A*T*A*G A**TT***G***G*****G*T----------**ATG**C****C*T*A*T*A*G A**TT***G***G*****G*T----------**ATG**C****C*T*A*T*A*G 1321 867 486 490 Av7-1 Av7-2 Av7-3 Av10 TTAAACTCCTACCTTTAATGTTTTCTTTCTGCCATTTTCATCATTTGTTTTTTT ***CGA*G**CTTG****AA**C*T***T-CTG*GC*----------AGC**** ***CGA*TG*CTTG****AA**C*T***T*CTG*GC*----------AGC**** ***CGA*TG*CTTG****AA**C*T***T*CTG*GC*----------AGC**** 1375 910 530 534 Av7-1 Av7-2 Av7-3 Av10 TTATGTTCAGTTGGTTAGTATGCGGCCCT-GTAGATGAAGGCCCTCGGTTCGAC *C*****************G*******T*C************************ GC*****************G*******T*C*A*************A******** GC*****************G*******T*C*A*************A******** 1428 964 584 588 Av7-1 TCCC---AGGATGCACAAATCTGCTTTGACTTCTCTTTGTC------------- 1466 Av7-2 ****---*****C*****G**********************CGTGTAGCTACAG 1015 Av7-3 ****TGC***T*T*****G**********************CGTGTAGCTACAG 638 Av10 ****TGC***T*T*****G**********************CGTGTAGCTACAG 642 Av7-1 Av7-2 Av7-3 Av10 -TTAAATACCTTTGTAAAAGAGCACTGATGGTTTGAGGGGGTCAGTAAAGTGCT C************A***G-************A*****C*T************** C*******A********G-*********************************** C*******A********G-*********************************** 1519 1068 691 695 Av7-1 Av7-2 Av7-3 Av10 CACCT-------CGATTGCTTTTAAACTATTTGGTAATCAACCTACTCCTTTAC *****TAAAATT*********--*********************-********* *****TAAAATT*********--*********************-********* *****TAAAATT*********--*********************-********* 1566 1119 742 746 Av7 S1D (part 2) Av7 S2D (part 1) Av7-1 Av7-2 Av7-3 Av10 (D) E P A F K D L N Q CTATGCCTTTTTTTTTC-AGACGAGCCGGCATTTAAAGACCTCAACCAAGGTAT ***************C*C************************************ ***************CA--*********************************** *****************--*********************************** 1619 1173 794 798 Av7-1 Av7-2 Av7-3 Av10 CGTATAAAGTGTTTAAG-TTATCGAATTTTAGTGGAAACTGACAATGCGAAAAT *****************-**************************T**T*****A *****************C*********************************G** ****************************************************** 1672 1226 848 852 Av7-1 Av7-2 Av7-3 Av10 AATATATTC-----TTGCAATAAAGTCACTTTGGTGTTTCCAAAGAACAAATCC *********ATGTC**************************************** *********-----**************************************** *********-----**************************************** 1721 1280 897 901 Av7-1 Av7-2 Av7-3 Av10 ATTAATATGATGTCATTCAGGAGAAGAAAACTGAAGGAATTATTAAACTCCTG********A**********************************A***G****AG **********************************************************************************************************- 1774 1334 950 954 Av7-1 Av7-2 Av7-3 Av10 -CTTTAATGTTTTCTTTCTGCCATTTTCATCATTTGTT-GTTTTTTTTATACTT CT*******************T*****T**********TA************** -*************************************-*************** -**************C**********************-*************** 1826 1388 1002 1006 Av7 S2D (part 2) Av7-1 Av7-2 Av7-3 Av10 (G)D E S L G K GCATTTTAGGTGACGAAAGTTTGGGAAAAAGTAAGTATTCATGCAAAGCATGCC *****************************************************T ****************************************************** ****************************************************** 1880 1442 1056 1060 Av7-1 Av7-2 Av7-3 Av10 ATTCGTTGCCTTAATTAATATTTTTCAATATTTT-AGTGAGAA-GGTAAATGTG **********************************T********A********** ******-***************************-********-********** **********************************-********-********** 1932 1496 1107 1112 Av7-1 Av7-2 Av7-3 Av10 ATGTAATCGAAGGACTCAAGGACTTTGATGGATGTGACACGTCAGTTTGAGAAG ****************************************************** ****************************************************** ****************************************************** 1986 1550 1161 1166 Av7-1 Av7-2 Av7-3 Av10 TGTGAGAAGAGTAGCCGTCTTGTGTGTTAATGCCACGGCGTGCTTGAAAACGGG ******G***********G*******C*******G******************* ************G*****G*********************************** ******************G*********************************** 2040 1604 1215 1220 Av7-1 Av7-2 Av7-3 Av10 GGGGAGATAGGGGATGTAGCCAAGTACCGGGGCGTCAGTGAAGGGCATCGGATT **--************************************************** **--************************************************** **--************************************************** 2094 1656 1267 1272 Av7-1 Av7-2 Av7-3 Av10 AGTCGTAAGATTAGCTACAGTTTTTTCATGCGCAGGTTGCATTTTTAAAAGCTG ***************-----------------------------------*TA* ************************************GC*************T** ************************************GC*************T** 2148 1675 1321 1326 Av7-1 Av7-2 Av7-3 Av10 ACGTACTTCAGCAACCATTCACACACTGTGGACCAGGCACTGCTCCATCTTCAA ***************************************************G** Av7 L2D ****************************************************** ****************************************************** 2202 1729 1375 1380 Av7-1 Av7-2 Av7-3 Av10 GCATTTGGGGAAGTGTATGGATCACTGATGCTAATTGTTTATTCAGTAAACTTG *************C******************G**********A********** ****************************************************** ****************************************************** 2256 1783 1429 1434 Av7-1 Av7-2 Av7-3 Av10 CCAGATTTGCATACACAGGAAACGTTTTCAATTTCCTAAATGGGAACTTTGGGA ***************************************C************** ****************************************************** ****************************************************** 2310 1837 1483 1488 Av7-1 Av7-2 Av7-3 Av10 AATTATTGGGAAATCACAACCCAAATTTGGGAAAATCTTTTTGAAATGAAATTT ******************************************T*********** ****************************************************** ****************************************************** 2364 1891 1540 1545 Av7 L1D Av7-1 Av7-2 Av7-3 Av10 TTTCCAAACCTTGGATTTTGTTGCATTTTCCCAATCATGGGAAGTTATGTGAAT ****************************************************** ***********C****G**************************T********** ***********C**G*G**************************T********** 2418 1945 1591 1596 Av7-1 Av7-2 Av7-3 Av10 TTCCCATAAAAGTCCCA--GAAATTACCCAAAACTTTGCCAGAATGGGGATCAT *****************--*********************************** *******T*******T*CT**************T******************** *******T*******T*CT**************T******************** 2470 1997 1645 1650 Av7 L2D Av7-1 Av7-2 Av7-3 Av10 GAAAAATTTCCTGTGATAGTTTGCCTCATTGGTATTATTGAGAGA----GTCAT *********************************************----***** *********************************************TAGA***** *********************************************TAGA***** 2520 2047 1699 1704 Av7 L3D Av7-1 Av7-2 Av7-3 Av10 Av7-1 Av7-2 Av7-3 Av10 Av7-1 Av7-2 Av7-3 Av10 (R)K S C C P C W L TCTTAATTCATTTGCTTATGTTTGCAGGGAAATCCTGCTGTCCTTGCTGGTTG 2573 ***************************************************** 2100 ***************************************************** 1752 ***************************************************** 1757 R/G G N C F W G Q N C Y P E/Q G C S G P CGCGGTAATTGCTTCTGGGGACAGAACTGCTATCCGGAAGGCTGCTCAGGCCCTA 2629 ******************************************************* 2156 ******************************************************* 1806 G****************************T******C****************** 1811 K V X AAGTATAATGGTCGAC 2644 **************** 2171 **************** 1823 **************** 1828 Supplementary Fig. 2 Nucleotide alignment of Av7 and Av10 genomic sequences. Identical residues are indicated by asterisks and gaps by hyphens. Exons are in red and introns are in black. Amino acid translation appears above the nucleotide sequence. Amino acids of the mature toxin are in blue. Parenthesized amino acids are encoded by nucleotides from two exons. The stop codon is denoted in the translation as "X". The location of the ATG start codon varies between the sequences and is indicated in bold and square parentheses of the first methionine which corresponds only to Av7-3 and Av10. Primers used in genome walking for differentiating between processed pseudogenes and ancestral Av7 genes are marked by magenta and orange arrows, respectively.
Source Exif Data:
File Type : PDF File Type Extension : pdf MIME Type : application/pdf PDF Version : 1.5 Linearized : Yes Author : MickeyG Create Date : 2009:06:03 20:00:38+03:00 Modify Date : 2009:06:03 20:00:38+03:00 XMP Toolkit : Adobe XMP Core 4.2.1-c041 52.342996, 2008/05/07-20:48:00 Creator Tool : PScript5.dll Version 5.2.2 Format : application/pdf Title : Microsoft Word - Supplementary File 2-JME.doc Creator : MickeyG Producer : Acrobat Distiller 9.0.0 (Windows) Document ID : uuid:dd5bfa16-0e0c-453e-abf3-32e120bc68ce Instance ID : uuid:f9bc75e9-9b93-4496-b7f3-e524c6f0e175 Page Count : 6EXIF Metadata provided by EXIF.tools