500BP Mailer
User Manual: 500BP
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• Kits for Total RNA from Blood • Kits for Total RNA from Viruses • Twin DNA/RNA Kit Mouse Tails,Yeast • Genomic Kits: for Blood,Tissues, • ‘Viral’ DNA Kit Qty 100ml 500ml 1000ml 100ml 500ml 1000ml 100ml 500ml 1000ml 3 £23.10 £42.90 £72.60 £20.90 £37.40 £66.00 £30.80 £51.70 £75.90 FOR Buy any 3 products, get lowest priced item free! Molecular Biology Grade Liquid Phenol Cat.No. Descirption 1690Q825 Liquid/Tris Stabilized 1690Q105 Liquid/Tris Stabilized 1690Q405 Liquid/Tris Stabilized 1690M825 Liquid/Stabilized 1690M105 Liquid/Stabilized 1690M405 Liquid/Stabilized 1690Cl82 Liquid/Ch/IAA 1690Cl10 Liquid/Ch/IAA 1690Cl40 Liquid/Ch/IAA Bio/PhenolTM: M I D A S : N U C L E I C AC I D P U R I F I C AT I O N T E C H N O L O G Y ∆ Blood, Cells and Tissues, Buffy Coat, Gram-ve Bacteria ∆ 120 Samples/day ∆ 2.4ml – 10ml Samples ∆ High Purity Genomic DNA, OD 260/280 ratio 1.75-1.90 Bio/PrepTM: Automated Genomic Preps: ALSO AVAILABLE: § Spin column speed & convenience with anion exchange quality DNA!! π Prices calculated pro-rata 2 Oligotex mRNA Mini-Kits (12) RNAeasy Kit (50) 125.00 163.00 48 117 141.00 75.00 BG-Dmr-mr/20 BG-Dtr-CT-mi/50 BG-Fd220-100 Plasmid Mini Kit tip-20 (25) Plasmid Midi Kit tip-100 (25) Plasmid Maxi tip-500 (10) QIAEX II Gel Extract (50) QIAquick PCR Purification (50) QIAamp DNA Mini Kit (50) 71.00 116.00 109.00 60.00 60.00 96.00 58 22 34 43 43 28 90.00 190.00 130.00 42.00 42.00 150.00 PlasmidPure MiniPrep (50) π PlasmidPure MidiPrep (50) π PlasmidPure MaxiPrep (16) π CleanIt Gel/PCR Kit (50) 1 Kit, 2 Applications! Genomic DNA from Cells, Tissues and Blood: ‘Forensic’ DNA Kit (100) π Direct mRNA Mini (20) π Total RNA C+T Kit Mini (50) QIAprep Spin Kit (50) 50.00 BG-PL-mi/50 § BG-PL-md/50 § BG-PL-ma/16 BG-CL/50 28 39.00 Plasmid Spin (50) Product (Preps) List Price £ BG-SP-50 Offer Price £ Qiagen Product (Preps) Price Difference (%) MidasTM vs Qiagen π Catalogue Number MIDASTM MIDASTM – HIGHER YIELD & PURITY PLUS SIGNIFICANTLY MORE COST EFFECTIVE THAN THE MARKET LEADER! BioGene House • 6 The Business Centre • Harvard Way • Kimbolton • Cambs. • PE28 0NJ tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com Hot Start Taq at the price of standard Taq! Take two of the top selling chemical Hot Start Taqs on the market and compare it to BioGene’s new Hot Taq... 1 2 3 4 5 6 7 8 9 10 No need to mind your P’s or Q’s ! Lane Lane Lane Lane Lane Lane Lane 1 + 10 2 3 4/5 6 7 8/9 DNA Marker Un-activated enzyme P Un-activated enzyme Q Un-activated HotTaq Activated enzyme P Activated enzyme Q Activated HotTaq Amplification of 540bp Human CD4 ... and if you can see a difference, it will only be in the price! HotTaq OFFER PRICE PRICE/250u 250u 2500u £62.50 £550.00 £62.50 £55.00 Complete with ‘Magic Reagent’ (‘MR’) for amplification of high GC content targets Enquire for pricing on larger pack sizes Bio/ChemzTM MOLECULAR BIOLOGY REAGENTS DTT GITC X-Gluc Caesium Chloride 1gm X-Gal (1gm IPTG FOC) £28.00 5gm X-Gal (5gm IPTG FOC) £135.00 5gm 500gm 1gm 1kg £18.00 £65.00 £94.50 £137.00 REAL TIME PCR REAGENTS *LightCycler User? **Taqman User? If you do not already buy your reagents from us, we guarantee minimally a 30% price difference on Real-Time PCR Master Mixes, with at least equivalence in absolute Quality of reagents, compared to your current supplier.We have been supporting LightCyclers since 1997. recruitment recruitment recruitment Give us a call! We are looking forrecruitment Regionalrecruitment Sales Managers! A L S O AVA I L A B L E Single Tube RT-PCR Kit Assay Development Application Trouble-shooting *Lightcycler is a trademark of Idaho Technology Inc. **Taqman is a trademark of H. La Roche recruitment recruitment recruitment recruitment recruitment Ph D Molecular Biologist? recruitment recruitment recruitment recruitment recruitment An aptitude for sales? recruitment recruitment recruitment recruitment recruitment Want to use your Science and get well remunerated? recruitment recruitment recruitment recruitment recruitment recruitment recruitment recruitmentand recruitment recruitment Well organised, self motivated enjoy a challenge? recruitment recruitment recruitment recruitment recruitment LOOK AT OUR WEBSITE recruitment recruitment recruitment recruitment recruitment via site map: ‘recruitment’ or email your cv to ‘recruitment@biogene.com’ recruitment recruitment recruitment recruitment recruitment BioGene House • 6 The Business Centre • Harvard Way • Kimbolton • Cambs. • PE28 0NJ tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com www.biogene.co.uk new ..... excellence and innovation in molecular biology REAL-TIME, CLOSED TUBE DETECTION OF NUCLEIC ACIDS Real-Time! Monitor your amplification “as it happens”, quantitate starting template and discriminate products without running an agarose gel! Use a centrifugal air thermal cycler for rapid results! Real Time Quantitation Use Standard 0.2ml Tubes! No more expensive glass capillaries which are difficult to handle and prone to breakage resulting in release of cross-contaminating aerosols. No Heating Blocks! So no uneven intra-block temperature gradients. Isothermic chamber gives rise to only specific, reproducible product amplification, every tube, every time! Multiplex Reactions! Using the two separate excitation channels, to ensure no spectral overlap between channels [470nm (blue), 530nm (green)]. Use Any Chemistry! Pick the best chemistry for your application not your machine! Use non-specific probes like Sybr-Green (melting point analysis and quantitation) or Sequence Specific Chemistries e.g. Hydrolysis, Hybridisation, “Scorpions”, “Molecular Beacons”. APPLICATIONS: Transcript Quantitation, Pathogen Detection, Genotyping. New Web Site: Become a “Bio/Club” member (free membership) then log-on for: Rotorgene Real Time Analyser AN ISO 9002 COMPANY • • • • Upto 50% Discounts! Application Support! Competitive, Comparative Pricing! On-Line Ordering! www.biogene.com To Obtain Special Offer Pricing Please Quote Ref SP2C4, offers expire 29/02/04 FM39343 The Polymerase Chain Reaction (PCR) is protected by a patent. The patent is held by Hoffmann-La Roche. BioGene House • 6 The Business Centre • Harvard Way • Kimbolton • Cambs. • PE28 0NJ tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com MICROARRAY TECHNOLOGY FROM V&P SCIENTIFIC Glass Slide Indexing System 9 x 384 Array/Registration MICROARRAY PRODUCTS • 96/384/1536/‘Custom’ Format • Replicators (fixed/floating pin) • Tumble Stirrers for MTP • Dispensers • Manual/‘Add to Robot’ Format • Standard/Deep Well MTP • Library Copiers • Colony Pickers • Rotary Magnetic Tumble Stirrers • Sterile/Autoclavable Option 96/384 Replicator Kit 384 Multi-Dispenser SEE COMPLETE RANGE ON www.biogene.com 15% DISCOUNT IF ORDERED ON-LINE GenHunter DIFFERENTIAL DISPLAY KIT A proven technique f o r identification & characterisation o f d i f f e re n t i a l l y expressed genes. Empirical schematic • Equivalent sensitivity to 33P • Excellent reproducibility • Only 5µg total RNA required new RNA Spectra Kits for fluorescent differential display. RNA Spectra Kit vs 33P labelling BioGene House • 6 The Business Centre • Harvard Way • Kimbolton • Cambs. • PE28 0NJ tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com LICENSED AMPLIFICATION REAGENTS! Products that have been tried and tested over the past 10 years worldwide: exceptional reagents at an exceptional price! PRODUCT CATALOGUE NO. PACK SIZE £ 500u 2,500u 10,000u 90.00 80/500u 70/500u 4 x 250µl 4 x (4 x 250µl) 1 x 1,000µl 70.00 240.00 38.00 DNA Polymerases:choose from • Taq, Tbr, Tfl, Tsp, Tth • ** 1.5mM Buffer (A) or separate Mg2+ + 10x PCR Buffer (B) D1002-Y(A/B)** D1002-Y(A/B)**/2500u D1002-Y(A/B)**/10000u Ultra Pure dNTP’s:• dNTP Set (100mM) 300-111 300-111 300-112 • DNA Polymerisation Mix (5mM) Master Mixes: includes optimised buffer, Taq, dNTP’s : ‘Ready-To-Use’ • Taq, Master Mix I • Taq, Master Mix III PCRM001 PCRM003 80 x 50µl PCR’s 1,000 x 50µl PCR’s 33.00 380.00 CalypsoTM: Single Tube RT-PCR Kit Bio/ScriptTM RNAse H- MMuLV-RT RT002 MMuLV-RT H- 40x 50µl Reactions 10,000u 120.00 70.00 Markers 10,000bp 8,000bp 6,000bp 5,000bp 4,000bp 3,000bp 2,500bp 2,000bp 1,500bp 1,000bp MH001 DNA Marker I 100µg Hind III digested lambda DNA £20.00 - MEH001 DNA Marker II 100µg EcoR/Hind III digested lambda DNA £20.00 MPL001 DNA Marker III 50µg of Nci I digested pMJ3 mixed with Taq I digested pHC624 £20.00 Bio/1kb DNA Ladder Size: 400 lanes (1µl/lane) 0.8% Agarose gel in 0.5X TBE buffer 500bp - £52.50 1 - 3,000bp - 2,000bp - 1,500bp Bio/100 bp DNA Ladder Size: 50 lanes (5µl/lane) 2% Agarose gel in 0.5X TAE buffer 3 FOR 2 - 1,000bp 900bp 800bp 700bp 600bp 500bp 400bp 300bp 200bp 100bp £41.50 “Thermal Cyclone”! 30 Cycles in Less than 10’! 2 The “Rapidcycler” has no metal block to impede heat transfer. Amplify a 500bp product in 9’30”! • • • • • Lower reagent costs - sample size as low as 10µl Cleaner reaction products 1 No oil overlay required No temperature variation from sample to sample due to its truly isothermic sample chamber Rapid throughput A 536bp product, from the human ß-globin gene is visible in lane 2. The Rapidcycler was programmed for 30 cycles with the following parameters: 94ºC for 0 sec; 60ºC for 0 sec; 72ºC for 5 sec. The reaction was completed in 9min 25 sec. 1 Wittwer et al 1991, Biotechniques 10: 76-83 BioGene House • 6 The Business Centre • Harvard Way • Kimbolton • Cambs. • PE28 0NJ tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com MOLECULAR BIOLOGY GRADE ‘ANALYSIS’ REAGENTS Bio/Resolve AgaroseTM A complete family of the highest specification agaroses. All products feature: absence of DNAses/RNAses, low DNA binding, good handling properties and low background fluorescence after Et. Br. Staining. Hi-PureTM: Molecular Biology Grade Agarose *PhoreCus™ Molecular Gating Agarose Cat No 300-200 300-600 300-650 Use for DNA fragments between 60-1000bp and where high gel strength (e.g. for large gels) is required. Qty 100gm 2x250gm 1Kg 2% TBE £ 38.00 135.00 240.00 Cat No. 300-900 £65/50g *Equivalent to MetaphorTM Hi-PureTM 1% TBE Lane Lane Lane Lane Lane Lane 1 2 3 4 5 6 y E1/H3 y H3 y Bst E2 564-21 Kb 564-23 Kb 700-8 Kb 5µl DIY Marker 250-5 Kb 1Kb Marker pHJ/pHL 624 ≥600bp 1 2 3 4 5 6 1 2 3 4 5 6 *Pure-Sieve LM™ PhoreCusTM NEW A low melting Agarose with high resolving capability for DNA fragments in the 200-800bp range. Use for in-gel enzymatic processes and preparative electrophoresis. Cat No Qty £ 300-300 50g 60.00 Hi-StrengthTM LMP Agarose Cat No Qty £ 300-750 50gm 75.00 *Equivalent to Nu-SieveTM Media LB Broth (pre-mixed) LB Agar (pre-mixed) Yeast Extract £5999 Cat No BCM-600 BCM-700 BCM-805 Qty 1Kg 1Kg 1Kg £ 36.00 55.00 39.90 3 FOR 2 Cat No Qty £ Agar 400-050 1Kg 72.00 Tryptone BCM-405 1Kg 47.50 * Nu-Sieve & Metaphor are trademarks of FMC Corporation Bio/VisionTM: High specification, compact, low-cost Gel-Documentation & Analysis System! System Configuration • 15 x 15cm UV plate for UV work (302nm). • UV to white light converter plate for protein work. • Low light integrating CCD camera, manual lens & UV filter. • IBM compatible PC, 15” monitor, frame-grabber. • Thermal Printer • Phoretix:‘Total Lab Software’ featuring simple to use yet powerful functions e.g. automatic band and lane identification, quantitation, dot-blots & array analysis.
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