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User Manual: 500BP

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§Spin column speed & convenience with anion exchange quality DNA!!

πPrices calculated pro-rata

MIDASTM – HIGHER YIELD & PURITY PLUS SIGNIFICANTLY

MORE COST EFFECTIVE THAN THE MARKET LEADER!

Bio/PhenolTM:

Molecular Biology Grade Liquid Phenol

Cat.No. Descirption Qty

1690Q825 Liquid/Tris Stabilized 100ml £23.10

1690Q105 Liquid/Tris Stabilized 500ml £42.90

1690Q405 Liquid/Tris Stabilized 1000ml £72.60

1690M825 Liquid/Stabilized 100ml £20.90

1690M105 Liquid/Stabilized 500ml £37.40

1690M405 Liquid/Stabilized 1000ml £66.00

1690Cl82 Liquid/Ch/IAA 100ml £30.80

1690Cl10 Liquid/Ch/IAA 500ml £51.70

1690Cl40 Liquid/Ch/IAA 1000ml £75.90

ALSO AVAILABLE:

• ‘Viral’ DNA Kit

• Genomic Kits:for Blood,Tissues,

Mouse Tails,Yeast

• Twin DNA/RNA Kit

• Kits for Total RNA from Viruses

• Kits for Total RNA from Blood

3 FOR 2

Price Difference (%)

MidasTM vs Qiagen π

117

BG-SP-50

BG-PL-mi/50 §

BG-PL-md/50 §

BG-PL-ma/16

BG-CL/50

BG-Fd220-100

BG-Dmr-mr/20

BG-Dtr-CT-mi/50

Plasmid Spin (50)

PlasmidPure MiniPrep (50) π

PlasmidPure MidiPrep (50) π

PlasmidPure MaxiPrep (16) π

CleanIt Gel/PCR Kit (50)

1 Kit, 2 Applications!

Genomic DNA from Cells,

Tissues andBlood:‘Forensic’

DNA Kit (100) π

Direct mRNAMini (20) π

Total RNA C+T Kit Mini (50)

39.00

90.00

190.00

130.00

42.00

150.00

141.00

75.00

50.00

71.00

116.00

109.00

60.00

96.00

125.00

163.00

QIAprep Spin Kit (50)

Plasmid Mini Kit tip-20 (25)

Plasmid Midi Kit tip-100 (25)

Plasmid Maxi tip-500 (10)

QIAEXIIGel Extract (50)

QIAquick PCRPurification (50)

QIAamp DNAMini Kit (50)

Oligotex mRNAMini-Kits (12)

RNAeasy Kit (50)

List Price

£Product (Preps)

Qiagen

Catalogue

Number Product (Preps)

MIDASTM

Offer Price

Bio/PrepTM:

Automated Genomic Preps:

∆Blood,Cells and Tissues,Buffy Coat,

Gram-ve Bacteria

∆120 Samples/day

∆2.4ml – 10ml Samples

∆High Purity Genomic DNA,OD

260/280 ratio 1.75-1.90

MIDAS: NUCLEIC ACID PURIFICATION TECHNOLOGY

Buy any 3 products,get lowest priced item free!

BioGene House •6 The Business Centre •Harvard Way •Kimbolton •Cambs. •PE28 0NJ

tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com

No need to mind your P’s or Q’s !

Lane 1 + 10 DNAMarker

Lane 2 Un-activated enzyme P

Lane 3 Un-activated enzyme Q

Lane 4/5 Un-activated HotTaq

Lane 6 Activated enzyme P

Lane 7 Activated enzyme Q

Lane 8/9 Activated HotTaq

... and if you can see a difference, it will only be in the price!

Complete with ‘Magic Reagent’ (‘MR’) for amplification of high GCcontent targets

Enquire for pricing on larger pack sizes

HotTaq

250u

2500u

OFFER PRICE

£62.50

£550.00

Bio/ChemzTM MOLECULAR BIOLOGY REAGENTS

1gm X-Gal (1gm IPTG FOC) £28.00

5gm X-Gal (5gm IPTG FOC) £135.00

DTT 5gm £18.00

GITC 500gm £65.00

X-Gluc 1gm £94.50

Caesium Chloride 1kg £137.00

Amplification of 540bp Human CD4

1 2 3 4 5 6 7 8 9 10

PRICE/250u

£62.50

£55.00

REAL TIME PCR REAGENTS

*LightCycler User? **Taqman User?

If you do not already buy your reagents from us,we guarantee minimally a 30% price

difference on Real-Time PCR Master Mixes,with at least equivalence in absolute

Quality of reagents,compared to your current supplier.We have been supporting

LightCyclers since 1997.

Give us a call!

*Lightcycler is a trademark of Idaho Technology Inc. **Taqman is a trademark of H. La Roche

ALSO AVAILABLE

Single Tube RT-PCR Kit

Assay Development

Application Trouble-shooting

Hot Start Taq

at the price of standard Taq!

Take two of the top selling chemical Hot Start Taqs on the market

and compare it to BioGene’s new Hot Taq...

recruitment recruitment recruitment recruitment recruitment

We are looking for Regional Sales Managers!

Ph D Molecular Biologist?

An aptitude for sales?

Want to use your Science and get well remunerated?

Well organised,self motivated and enjoy a challenge?

LOOK AT OUR WEBSITE

via site map:‘recruitment’ or email your cv to ‘recruitment@biogene.com’

Real-Time! Monitor your amplification “as it happens”,

quantitate starting template and discriminate products

without running an agarose gel! Use a centrifugal air

thermal cycler for rapid results!

Use Standard 0.2ml Tubes! No more expensive glass

capillaries which are difficult to handle and prone to break-

age resulting in release of cross-contaminating aerosols.

No Heating Blocks! So no uneven intra-block

temperature gradients. Isothermic chamber gives rise to

only specific, reproducible product amplification, every tube, every time!

Multiplex Reactions! Using the two separate excitation channels, to ensure no spectral overlap

between channels [470nm (blue), 530nm (green)].

Use Any Chemistry! Pick the best chemistry for your application not your machine! Use

non-specific probes like Sybr-Green (melting point analysis and

quantitation) or Sequence Specific Chemistries e.g. Hydrolysis,

Hybridisation,“Scorpions”, “Molecular Beacons”.

APPLICATIONS:

Transcript Quantitation,

Pathogen Detection,Genotyping.

New Web Site:

Become a “Bio/Club” member (free membership)

then log-on for:

•Upto 50% Discounts!

•Application Support!

•Competitive, Comparative Pricing!

•On-Line Ordering!

www.biogene.com

REAL-TIME, CLOSED TUBE DETECTION OF NUCLEIC ACIDS

new

BioGene House •6 The Business Centre •Harvard Way •Kimbolton •Cambs. •PE28 0NJ

tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com

www.biogene.co.uk

The Polymerase Chain Reaction (PCR) is protected by a patent. The patent is held by Hoffmann-La Roche.

To Obtain Special Offer Pricing Please Quote Ref SP2C4, offers expire 29/02/04

..... excellence and innovation in molecular biology

AN ISO 9002

COMPANY

FM39343

Real Time Quantitation

Rotorgene Real Time Analyser

BioGene House •6 The Business Centre •Harvard Way •Kimbolton •Cambs. •PE28 0NJ

tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com

MICROARRAY TECHNOLOGY FROM V&P SCIENTIFIC

SEE COMPLETE RANGE ON

www.biogene.com

15% DISCOUNT IF ORDERED ON-LINE

GenHunter DIFFERENTIAL

DISPLAY KIT

A proven technique

for identification

& characterisation

of differentially

expressed genes.

RNA Spectra

Kits

for fluorescent

differential display.

MICROARRAY

PRODUCTS

•96/384/1536/‘Custom’ Format •Manual/‘Add to Robot’ Format •Standard/Deep Well MTP

•Replicators (fixed/floating pin) •Library Copiers •Colony Pickers

•Tumble Stirrers for MTP •Rotary Magnetic Tumble Stirrers •Sterile/Autoclavable Option

•Dispensers

•Equivalent sensitivity to 33P

•Excellent reproducibility

•Only 5µg total RNA required

96/384 Replicator Kit

Glass Slide Indexing System 9 x 384 Array/Registration

384 Multi-Dispenser

Empirical schematic

RNA Spectra Kit vs 33P labelling

new

Markers

MH001 DNA Marker I

100µg Hind III digested lambda DNA £20.00

MEH001 DNA Marker II

100µg EcoR/Hind III digested lambda DNA £20.00

MPL001 DNA Marker III

50µg of Nci I digested pMJ3 mixed with Taq I digested pHC624 £20.00

BioGene House •6 The Business Centre •Harvard Way •Kimbolton •Cambs. •PE28 0NJ

tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com

LICENSED AMPLIFICATION REAGENTS!

Bio/1kb DNA Ladder

Size:400 lanes (1µl/lane)

0.8% Agarose gel in 0.5X TBE buffer

Products that have been tried and tested over the past 10 years worldwide:exceptional reagents at an exceptional price!

“Thermal Cyclone”!

30 Cycles in Less than 10’!

Bio/100 bp DNA Ladder

Size:50 lanes (5µl/lane)

2% Agarose gel in 0.5X TAE buffer

PRODUCT

DNA Polymerases:-

choose from

•Taq,Tbr,Tfl,Tsp,Tth

•** 1.5mM Buffer (A) or separate Mg2+

+ 10x PCR Buffer (B)

Ultra Pure dNTP’s:-

•dNTP Set (100mM)

•DNAPolymerisation Mix (5mM)

Master Mixes: includes optimised buffer, Taq, dNTP’s : ‘Ready-To-Use’

•Taq, Master Mix I

•Taq, Master Mix III

CalypsoTM:Single Tube RT-PCR Kit

Bio/ScriptTM RNAse H-MMuLV-RT

CATALOGUE NO.

D1002-Y(A/B)**

D1002-Y(A/B)**/2500u

D1002-Y(A/B)**/10000u

300-111

300-112

PCRM001

PCRM003

RT002

MMuLV-RT H-

PACK SIZE

500u

2,500u

10,000u

4 x 250µl

4 x (4 x 250µl)

1 x 1,000µl

80 x 50µl PCR’s

1,000 x 50µl PCR’s

40x 50µl Reactions

10,000u

90.00

80/500u

70/500u

70.00

240.00

38.00

33.00

380.00

120.00

70.00

£41.50

£52.50 3 FOR 2

The “Rapidcycler” has no metal block to impede heat

transfer. Amplify a 500bp product in 9’30”!

•Lower reagent costs - sample size as low as 10µl

•Cleaner reaction products 1

•No oil overlay required

•No temperature variation from sample to sample due

to its truly isothermic sample chamber

•Rapid throughput

A 536bp product, from the human ß-globin gene is visible

in lane 2. The Rapidcycler was programmed for 30 cycles

with the following parameters: 94ºC for 0 sec; 60ºC for 0

sec; 72ºC for 5 sec. The reaction was completed in 9min

25 sec. 1Wittwer et al 1991, Biotechniques 10: 76-83

10,000bp -

8,000bp -

6,000bp -

5,000bp -

4,000bp -

3,000bp -

2,500bp -

2,000bp -

1,500bp -

1,000bp -

500bp -

- 3,000bp

- 2,000bp

- 1,500bp

- 1,000bp

- 900bp

- 800bp

- 700bp

- 600bp

- 500bp

- 400bp

- 300bp

- 200bp

- 100bp

System Configuration

•15 x 15cm UV plate for UV work (302nm).

•UV to white light converter plate for protein

work.

•Low light integrating CCD camera, manual lens

& UV filter.

• IBM compatible PC,15”monitor,frame-grabber.

•Thermal Printer

• Phoretix:‘Total Lab Software’featuring simple to

use yet powerful functions e.g. automatic band

and lane identification, quantitation, dot-blots &

array analysis.

BioGene House •6 The Business Centre •Harvard Way •Kimbolton •Cambs. •PE28 0NJ

tel: +44 (0)1480 861831 • fax: +44 (0)1480 861899 • email: biogene@biogene.com • www.biogene.com

MOLECULAR BIOLOGY GRADE ‘ANALYSIS’ REAGENTS

Bio/Resolve AgaroseTM

A complete family of the highest specification agaroses.All products feature:absence of DNAses/RNAses,

low DNA binding,good handling properties and low background fluorescence after Et. Br. Staining.

2% TBE Hi-PureTM

Lane 1 E1/H3 564-21 Kb

Lane 2 H3 564-23 Kb

Lane 3 Bst E2 700-8 Kb

Lane 4 5µl DIY Marker 250-5 Kb

Lane 5 1Kb Marker

Lane 6 pHJ/pHL 624 ≥600bp

1% TBE PhoreCusTM

Hi-PureTM:Molecular Biology Grade Agarose

Cat No Qty £

300-200 100gm 38.00

300-600 2x250gm 135.00

300-650 1Kg 240.00

*PhoreCus™ Molecular Gating Agarose

Use for DNA fragments between 60-1000bp and where high

gel strength (e.g. for large gels) is required.

Cat No. 300-900 £65/50g

*Equivalent to MetaphorTM

*Pure-Sieve LM™

A low melting Agarose with high resolving capability for DNA

fragments in the 200-800bp range. Use for in-gel enzymatic Hi-StrengthTM LMP Agarose

processes and preparative electrophoresis. Cat No Qty £

Cat No Qty £ 300-750 50gm 75.00

300-300 50g 60.00

Media Cat No Qty £Cat No Qty £

LB Broth (pre-mixed) BCM-600 1Kg 36.00 Agar 400-050 1Kg 72.00

LB Agar (pre-mixed) BCM-700 1Kg 55.00 Tryptone BCM-405 1Kg 47.50

Yeast Extract BCM-805 1Kg 39.90

Bio/VisionTM:High specification, compact,

low-cost Gel-Documentation & Analysis System!

FOR

NEW

£5999

* Nu-Sieve & Metaphor are trademarks of FMC Corporation

1 2 3 4 5 6 1 2 3 4 5 6

*Equivalent to Nu-SieveTM

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