Manual Beginner Mac
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CRISPRpic Manual for Beginners (Mac OS) Written by Seung Woo Cho In order to run CRISPRpic, you will need the Anaconda Python. It is like a PCR-master mix, the regular Python is like a polymerase. Don’t worry. It is not harmful to your computer. 1. Run Terminal App in MacOS 2. Type “Python” to check the Anaconda was installed. 3. ctrl+z to exit from Python. This Python 2.7.10 is provided by MacOS. You will install the Anaconda. 1. https://www.anaconda.com/download/#macos 2. Click Python 3.6 version 64-bit command line installer 3. 4. 5. 6. 7. Open Terminal App. Move to Downloads folder Type “Bash Anaconda3-5.2.0-MacOSX-x86_64.sh Follow the instruction from Anaconda After install, you must close and re-open the Terminal App prior to use the Anaconda. See this page for details. https://docs.anaconda.com/anaconda/install/mac-os#macos-graphical-install 8. Type “Python” to verify the Anaconda installation. Now, your computer is ready to run CRISPRpic. You can download the CRISPRpic at https://github.com/compbio/CRISPRpic 1 2 1. Unzip the CRISPRpic-master.zip 2. Move to CRISPRpic-master/TEST 3. Type “Python ../CRISPRpic.py -i AAVS1_input.txt -f AAVS1.out.extendedFrags.fastq -w 3 4. The output files will be generated in /CRISPRpic/TEST/AAVS1/
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