Présentation Manual Analysistool
Manual_analysistool
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Analysis Tool Manual Isam BEN SOLTANE Files Selection Tab Files stored in projects: • • • Local folders containing modified versions of the selected files Creating a project: Create an empty local folder Load a project: Load the files and the information of a project formerly created SAVE THE PROJECT AFTER ADDING ALL THE FILES 2 Files Selection Tab Creates a new project named test Add local files to the project: • ENRICHED FCS FILE • REQUIRED KEYWORDS Use Clustering Tool or Keywords Registor 3 Files Selection Tab Select the column containing the populations labels 4 Rename each population OR Or load a mapping file (.csv) eg: scaffold node landmarks Files Selection Tab Selected Files: • Download the selected files stored in each project • Remove all the selected files and their information from the project 5 Files Selection Tab Save Project: • Save all the files and their information in the folder associated with the project 6 Scoring Tab Run Analysis Button: Compute the purity, precision, recall, FG matrices and clusters information for each registered set of parameters, for each algorithm Scoring Tab Visualization Panel : Annotations Visualization Select a file (only one in this case) Select an algorithm among those used on the chosen file Select a set of parameters of the chose selected algorithm Scoring Tab Visualization Panel : Annotations Visualization Select the markers to visualize Selected Populations : Markers expression Plot Selected Annotations : Markers expression Scoring Tab Visualization Panel : F-score variations Select a file (only one in this case) Select an algorithm among those used on the chosen file Scoring Tab Visualization Panel : F-score variations Select the variable parameter Evolution of the F-score of all populations: • Depending on the values of the variable parameter • Each population is identified by a color • The maximum value of the cumulated F-score is the number of populations Plot Select the values of the other parameters Scoring Tab Visualization Panel : F-score variations Select a set of parameters Plot • F-Score of each population for the selected set of parameters • The relative size gives the ratio number of events in the file size of population Scoring Tab Visualization Panel : Summary table Summary Table: Sum of the F-Score of all populations for every file, every algorithm with every set of parameters Scoring Tab Visualization Panel : Advanced Options • • • Purity of each cluster relative to their population. The clusters are grouped into populations The purity threshold sorts clusters in the two tables on the left
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