Présentation Manual Analysistool

Manual_analysistool

User Manual:

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Page Count: 14

Analysis Tool
Manual
Isam BEN SOLTANE
Files Selection Tab
2
Files stored in projects:
Local folders containing modified versions of the selected files
Creating a project: Create an empty local folder
Load a project: Load the files and the information of a project formerly created
SAVE THE PROJECT AFTER ADDING ALL THE FILES
Files Selection Tab
3
Creates a new project
named test
Add local files to the project:
ENRICHED FCS FILE
REQUIRED KEYWORDS
Use Clustering Tool or Keywords Registor
Files Selection Tab
4
Select the column containing the
populations labels Rename each population Or load a mapping file (.csv)
eg: scaffold node landmarks
OR
Files Selection Tab
5
Selected Files:
Download the selected files stored in each project
Remove all the selected files and their information from the project
Files Selection Tab
6
Save Project:
Save all the files and their information in the folder associated with the project
Scoring Tab
Run Analysis Button:
Compute the purity, precision, recall, FG matrices and clusters information for each
registered set of parameters, for each algorithm
Scoring Tab
Select a file (only one in this case)
Select an algorithm among those used
on the chosen file
Select a set of parameters of the chose
selected algorithm
Visualization Panel : Annotations Visualization
Scoring Tab
Select the markers to visualize Plot
Selected Populations :
Markers expression
Selected Annotations :
Markers expression
Visualization Panel : Annotations Visualization
Scoring Tab
Select a file (only one in this case)
Select an algorithm among those used
on the chosen file
Visualization Panel : F-score variations
Scoring Tab
Select the variable parameter Plot Select the values of the other parameters
Visualization Panel : F-score variations
Evolution of the F-score of all populations:
Depending on the values of the
variable parameter
Each population is identified by a
color
The maximum value of the
cumulated F-score is the number of
populations
Scoring Tab
Select a set of parameters Plot
Visualization Panel : F-score variations
F-Score of each population for the selected set of
parameters
The relative size gives the ratio size of population
number of events in the file
Scoring Tab
Visualization Panel : Summary table
Summary Table:
Sum of the F-Score of all populations for every file, every algorithm with every set of parameters
Scoring Tab
Visualization Panel : Advanced Options
Purity of each cluster relative to their population.
The clusters are grouped into populations
The purity threshold sorts clusters in the two tables on the left

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