Quick Reference Phlebotomy Guide
User Manual:
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Disclaimer: The Quick Reference Phlebotomy Guide is an original work by R.E. Sprouse designed strictly as a writing sample and/or portfolio piece. This work may not be
reproduced, used, modified or distributed without written permission from R.E. Sprouse. The author is not affiliated with any existing company, business, individual or
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ME R C Y HE A L T H
UN I V E R S I T Y ME D I C A L CE N T E R
QUICK REFERENCE PHLEBOTOMY GUIDE
Laboratory Blood Tube Collection
BLOOD CULTURES
Routine
5 – 10 mL / bottle
Collect Aseptically
Bl and Pu topped tubes are a set
AFB
5 – 10 mL / bottle
Collect Aseptically
Call Microbiology at 2-3125
Pediatrics
1 – 4 mL / bottle
BLOOD BANK TESTING
The Collector and Witness are
required during collection
Use a 6 mL EDTA tube
Optional: La or Rd tops
ICE TRANSPORT
Ammonia and Lactate test samples
require an ice transport
ORDER OF DRAW
Blood Cultures are always drawn first
When using the vacutainer system:
Wh → Bl → Go → Rd → Gn (gel) →
La → Pi → Gn → Gy
When using a syringe and safety
transfer device:
Bl → La → Go → Gn (gel) → Rd →
Pi → Gy
Mix all tubes 4 to 5 times as soon as
they are filled
HEMATOLOGY
The tube must be full for Sed Rate/ESR
and Bl topped tubes
Call Hematology at 2-3174 for
Bleeding Time and PFA
GUIDE KEY
Review the LABORATORY SPECIMEN COLLECTION AND HANDLING GUIDELINES
policy for detailed instructions of proper specimen collection, handling and transportation.
CODE
COLOR or TUBE
Bl
Blue
Bk
Black
Gn
Green
Go
Gold
Gy
Gray
La
Lavender
Pi
Pink
Pu
Purple
Rd
Red
ST
Special Tube
Wh
White
Yw
Yellow
TEST
TUBE
DEPARTMENT
AFB Blood Culture
Bk
Microbiology
Ammonia
Gn
Chemistry
Basic/Comprehensive Panels
Yw
Chemistry
Blood Bank Testing
Pi
Blood Bank
Blood Cultures, Routine
Bl
Pu
Microbiology
Blood Cultures, Pediatrics
Yw
Microbiology
B-NP
Pu
Chemistry
CBC with Diff
La
Hematology
CBC
La
Hematology
CPK
Yw
Chemistry
CPKMB/Troponin
Gn
gel
Chemistry
D-Dimer
Bl
Hematology*
Electrolytes
Yw
Chemistry
Fibrinogen
Bl
Hematology*
Glucose
Yw
Chemistry
*Call Hematology at 2-3174
TEST
TUBE
DEPARTMENT
HCG
Yw
Chemistry
Hemoglobin/Hematocrit
La
Hematology
Hemoglobin A1c
La
Chemistry
Heparin, LMWH
Bl
Hematology*
Heparin, Unfractionated
Bl
Hematology*
Intact PTH
Yw
Chemistry
Lactate
Gy
Chemistry
Platelet Count
Lw
Hematology
Plavix
ST
Hematology*
Prothrombin Time (PT)
Bl
Hematology*
PSA
Yw
Chemistry
PTT
Bl
Hematology*
Retic
La
Hematology
Sed Rate/ESR
La
Bk
Hematology*
Thyroid Tests
Rd
Chemistry
