1005198_Sequencing_LTW_SingleRead_GA2_v3 Sequencing LTF Single Read GA2 1005198 Rev B
Sequencing_LTF_Singl.. Sequencing_LTF_SingleRead_GA2_1005198_RevB
User Manual: Sequencing LTF SingleRead GA2 1005198 RevB
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Single-Read Sequencing on the Genome Analyzer II Lab Tracking Form 1 Flow cell ____________________ Run folder ____________________ Samples Loaded Lane 2 Sample ID Concentration (pM) 1 Sam ple 1 Con c. 1 2 Sam ple 2 Con c. 2 3 Sam ple 3 Con c. 3 4 Sam ple 4 Con c. 4 5 Sam ple 5 Con c. 5 6 Sam ple 6 Con c. 6 7 Sam ple 7 Con c. 7 8 Sam ple 8 Con c. 8 Cluster Generation Step Date Amplification Linearization, Blocking, and Primer Hybridization Single-Read Cluster Generation Kit v2 Lot Numbers Reagent Lot Number AMX1 AT1 BMX EMX HP3 HP4 HT1 HT2 LS1 Part # 1005198 Rev. B Page 1 of 5 Single-Read Sequencing on the Genome Analyzer II Lab Tracking Form Reagent Volumes After Amplification Position Reagent 1 AMX1 3 HT2 9 AT1 12 HT1 Volume Reagent Volumes After Linearization, Blocking, and Primer Hybridization 3 Position Reagent 3 HT2 4 BMX 7 HP1 12 HT1 15 LS1 17 HP3 Volume Preparing Sequencing Reagents SBS Sequencing Kit v3 Lot Numbers Reagent Lot Number IMX FFN SDP CLM SMX PR1 PR2 PR3 PW1 Part # 1005198 Rev. B Page 2 of 5 Single-Read Sequencing on the Genome Analyzer II Lab Tracking Form 4 Pre-Run Wash Expected Volume Delivered Volume 7 ml 5 Preparing, Loading, and Priming Reagents Weights of Material Delivered Position Solution Weight before Run Weight after Run 1 IMX 0.00 2 PW1 0.00 3 SMX 0.00 4 PR1 0.00 5 PR2 0.00 6 CLM 0.00 7 PR3 0.00 Priming Reagents Expected Volume Delivered Volume 6.4 ml 6 % Difference -100.00 Checking for Leaks and Reagent Delivery Port Position 5 Expected Volume: 800 μl Measured Volume 1: ____________________ Measured Volume 2: ____________________ Measured Volume 3: ____________________ % Difference Between Expected Volume and Measured Volume 3: -100 ____________________ Part # 1005198 Rev. B Page 3 of 5 Delivered Weight Single-Read Sequencing on the Genome Analyzer II Lab Tracking Form 7 Setting Best Focus for Channel A Z Position FQ value 1 A300 1 C300 2 A200 2 C200 3 A100 3 C100 4 A0 4 C0 5 A-100 5 C-100 6 A-200 6 C-200 7 A-300 7 C-300 Position of Z for Lane 4: 8 _____________________ Adjusting Focus FQ Calculation Z Position A C G T Total A70 0 C70 0 G70 0 T70 0 TOT7 00 A60 0 C60 0 G60 0 T60 0 TOT6 00 A50 0 C50 0 G50 0 T50 0 TOT5 00 A40 0 C40 0 G40 0 T40 0 TOT4 00 300 A30 0 C30 0 G30 0 T30 0 TOT3 00 200 A20 0 C20 0 G20 0 T20 0 TOT2 00 100 A10 0 C10 0 G10 0 T10 0 TOT1 00 0 A0 C0 G0 T0 TOT0 -100 A100 C100 G100 T100 TOT100 -200 A200 C200 G200 T200 TOT200 -300 A300 C300 G300 T300 TOT300 A400 C400 G400 T400 TOT400 A500 C500 G500 T500 TOT500 A600 C600 G600 T600 TOT600 A700 C700 G700 T700 TOT700 Part # 1005198 Rev. B Page 4 of 5 Single-Read Sequencing on the Genome Analyzer II Lab Tracking Form 9 Performing Post-Run Procedures Run Fluid Delivery Data Lane Weight of Empty Tube Weight of Tube after Run Weight of Fluid Delivered during Run 0.00 0.00 1 2 4 0.00 0.00 5 0.00 6 0.00 7 0.00 8 0.00 3 Post-Run Wash Weight of Tube after Wash: ____________________ Weight of Empty Tube: ____________________ Weight of Fluid Delivered: 0 ____________________ 10 Sign-off Date: ____________________ Name: ____________________ Signature: ____________________ Part # 1005198 Rev. B Page 5 of 5
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