1005198_Sequencing_LTW_SingleRead_GA2_v3 Sequencing LTF Single Read GA2 1005198 Rev B
Sequencing_LTF_Singl.. Sequencing_LTF_SingleRead_GA2_1005198_RevB
User Manual: Sequencing LTF SingleRead GA2 1005198 RevB
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Page Count: 5
Single-Read Sequencing on the Genome Analyzer II
Lab Tracking Form
Part # 1005198 Rev. B Page 1 of 5
Flow cell ____________________
Run folder ____________________
1 Samples Loaded
Lane Sample ID Concentration (pM)
1 Sam
ple
1 Con
c. 1
2 Sam
ple
2 Con
c. 2
3 Sam
ple
3 Con
c. 3
4 Sam
ple
4 Con
c. 4
5 Sam
ple
5 Con
c. 5
6 Sam
ple
6 Con
c. 6
7 Sam
ple
7 Con
c. 7
8 Sam
ple
8 Con
c. 8
2 Cluster Generation
Step Date
Amplification
Linearization, Blocking, and Primer
Hybridization
Single-Read Cluster Generation Kit v2 Lot Numbers
Reagent Lot Number
AMX1
AT1
BMX
EMX
HP3
HP4
HT1
HT2
LS1
Single-Read Sequencing on the Genome Analyzer II
Lab Tracking Form
Part # 1005198 Rev. B Page 2 of 5
Reagent Volumes After Amplification
Position Reagent Volume
1 AMX1
3 HT2
9 AT1
12 HT1
Reagent Volumes After Linearization, Blocking, and Primer Hybridization
Position Reagent Volume
3 HT2
4 BMX
7 HP1
12 HT1
15 LS1
17 HP3
3 Preparing Sequencing Reagents
SBS Sequencing Kit v3 Lot Numbers
Reagent Lot Number
IMX
FFN
SDP
CLM
SMX
PR1
PR2
PR3
PW1
Single-Read Sequencing on the Genome Analyzer II
Lab Tracking Form
Part # 1005198 Rev. B Page 3 of 5
4 Pre-Run Wash
Expected Volume Delivered Volume
7 ml
5 Preparing, Loading, and Priming Reagents
Weights of Material Delivered
Position Solution Weight before Run Weight after Run Delivered Weight
1 IMX
2 PW1
3 SMX
4 PR1
5 PR2
6 CLM
7 PR3
Priming Reagents
Expected Volume Delivered Volume % Difference
6.4 ml
6 Checking for Leaks and Reagent Delivery
Port Position 5
Expected Volume: 800 μl
Measured Volume 1: ____________________
Measured Volume 2: ____________________
Measured Volume 3: ____________________
% Difference Between
Expected Volume and
Measured Volume 3: ____________________
0.00
0.00
0.00
0.00
0.00
0.00
0.00
-100.00
-100
Single-Read Sequencing on the Genome Analyzer II
Lab Tracking Form
Part # 1005198 Rev. B Page 4 of 5
7 Setting Best Focus for Channel A
Z Position FQ value
1 A300 1 C300
2 A200 2 C200
3 A100 3 C100
4 A0 4 C0
5 A-100 5 C-100
6 A-200 6 C-200
7 A-300 7 C-300
Position of Z for Lane 4: _____________________
8 Adjusting Focus
FQ Calculation
Z Position A C G T Total
A70
0 C70
0 G70
0 T70
0 TOT7
00
A60
0 C60
0 G60
0 T60
0 TOT6
00
A50
0 C50
0 G50
0 T50
0 TOT5
00
A40
0 C40
0 G40
0 T40
0 TOT4
00
300 A30
0 C30
0 G30
0 T30
0 TOT3
00
200 A20
0 C20
0 G20
0 T20
0 TOT2
00
100 A10
0 C10
0 G10
0 T10
0 TOT1
00
0 A0 C0 G0 T0 TOT0
-100 A-
100 C-
100 G-
100 T-
100 TOT-
100
-200 A-
200 C-
200 G-
200 T-
200 TOT-
200
-300 A-
300 C-
300 G-
300 T-
300 TOT-
300
A-
400 C-
400 G-
400 T-
400 TOT-
400
A-
500 C-
500 G-
500 T-
500 TOT-
500
A-
600 C-
600 G-
600 T-
600 TOT-
600
A-
700 C-
700 G-
700 T-
700 TOT-
700
Single-Read Sequencing on the Genome Analyzer II
Lab Tracking Form
Part # 1005198 Rev. B Page 5 of 5
9 Performing Post-Run Procedures
Run Fluid Delivery Data
Lane Weight of Empty Tube Weight of Tube after Run Weight of Fluid Delivered
during Run
1
2
3
4
5
6
7
8
Post-Run Wash
Weight of Tube after Wash: ____________________
Weight of Empty Tube: ____________________
Weight of Fluid Delivered: ____________________
10 Sign-off
Date: ____________________
Name: ____________________
Signature: ____________________
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0
