DF1000 Verification Procedure For Accuracy And Precision
User Manual: DF1000
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Standard Operating Procedure for Pipettes Verification Procedure for Accuracy and Precision (defined for Users to incorporate in SOPs) In accordance with ISO8655 Standard Procedure LT802292/F - © 2007 Gilson SAS All rights reserved August 2007 Contents Glossary 3 Introduction 4 Environmental Conditions 5 Technician 6 Pipette Operation 6 Training 6 Pipette Tips 7 Test Equipment 8 Balance 8 Thermometer, Hygrometer and Barometer 8 Weighing Containers 9 Water 9 Verification Procedure 10 Procedure Summary 10 Multichannel Pipettes 10 Test Volumes 11 Estimating the Evaporation Rate (Mass Loss/Cycle) 12 Gravimetric Test 13 Calculations Formulae 14 Z-factor 15 Verification Procedure Report 16 Appendix 18 Associated Documents 22 Notes 23 In this document the word “tip” is used in the generic sense, where tip is the disposable part that must be used with the pipette: for Distriman this means “DistriTip”, for Microman “capillary-piston” and for Pipetman “Gilson Diamond Tip”. Page Glossary adjustment manufacture of an apparatus within appropriate tolerances, or the supplier’s setting of the apparatus, ensuring the metrological performance, as specified in the applicable part of ISO 8655. calibration set of operations that establish the relationship between the dispensed volume and the corresponding nominal or selected volume of the apparatus. maximum permissible error upper or lower permitted extreme value for the deviation of the dispensed volume from the nominal volume or selected volume of a piston-operated volumetric apparatus. systematic error difference between the dispensed volume and the nominal volume or selected volume of the piston-operated volumetric apparatus. random error scatter of the dispensed volumes around the mean of the dispensed volumes. uncertainty of measurement parameter, associated with the dispensed volume, that characterizes the dispersion of the volumes that could reasonably be attributed to the dispensed volume. nominal volume volume specified by the manufacturer and used for identification and for indication of the measuring range. Page Introduction This document describes a verification procedure for the following Gilson pipettes: Pipetman® Ultra (Single and Multichannel), Pipetman® Concept (Single and Multichannel), Pipetman® P, Pipetman® Neo, Pipetman® F, Pipetman® 8X200, Microman® and Distriman®. The procedure is for verifying pipette performance using gravimetric tests of repeated aspirate and dispense cycles with distilled water (grade 3, ISO 3696), in controlled conditions. The test conditions and methods described herein are fully compatible with ISO 8655 and are often stricter than those specified in the international standard, as are the expected results for maximum permissible errors, which are tabulated in the Appendix. So, adherence to this procedure assures conformity to Gilson’s specifications for accuracy (systematic error) and precision (random error) and to ISO requirements. The procedure, which for small volumes includes a correction for evaporation loss, evaluates the total system of pipetting: pipette, tip, and operator. Therefore the procedure must be carried out by suitably qualified and trained technicians. In calculating the volumes from balance readings, corrections are made for the temperature and air pressure when the test was made (Z-factor, refer to page 15). Although the document does not directly concern itself with other tests performed by the user, the method and calculations described herein may be applied in other tests, outside the scope of this document. Users shall establish a regular testing routine at least once a year for their piston pipettes according to: accuracy and precision requirements, frequency of use, number of operators using the pipette, number of operations on each occasion of use and the nature of the liquids being dispensed. In the case of Pipetman Ultra and Pipetman Concept, the number of cycles can be the basis of your regular testing routine. Page Environmental Conditions The test shall be carried out in a draught-free room with a stable environment. The test room (laboratory) shall have humidity and temperature control so that the atmospheric conditions of the environment where the procedure will take place and the temperature of the equipment used are stable and homogeneous before and during the procedure. The use of a chart recorder is recommended. The temperature of the pipettes being verified and the distilled water (grade 3, ISO 3696) used in the gravimetric test should have stabilized before the procedure commences. The pipettes, water and test apparatus should have been placed in the test room at least 2 hours before starting the tests. Ideally, verification takes place under the following conditions: 1) Temperature (t) ISO 8655 recommends that the gravimetric tests take place where the ambient and water temperature (t) are stable (± 0.5 °C) between 15 °C and 30 °C. Gilson recommends a range between 20 °C and 23 °C with a constant temperature (± 0.5 °C) between the beginning and the end of gravimetric tests. It is recommended to put water and pipettes at least 2 hours in the calibration room to reach an equilibrium with the room conditions. Z-factor is used to convert mass into volume according to temperature and pressure. 2) Relative humidity (RH) ISO 8655 states that the RH must be greater than 50%. However, Gilson recommends that a humidity range of between 50% and 75% be maintained throughout the verification procedure. In all cases, the evaporation rate will be evaluated for volumes 50 µL. 3) Barometric pressure Gilson’s tests should take place at 1013 ± 25 hPa. The barometric pressure in the test room shall be recorded to the nearest 0.5 kPa. Z-factor is used to convert mass into volume according to temperature and pressure. Page Technician Pipette Operation Consistency of pipetting technique contributes significantly to the reproducibility of the results of the Verification Procedure. Inexperienced technicians can cause substantial variations in apparent pipette performance. For meaningful test results, the technicians must be well-trained and qualified. You should operate the pipette according to the instructions given in the user’s guide of the pipette under test. Attention should be given to maintain a steady rhythm when aspirating and dispensing samples, speed and smoothness when pressing and releasing the push-button, and tip immersion depth. The test cycle time shall be kept to a minimum. It should not exceed 60 s. Training The Verification Procedure described in this document must be carried out by a suitably qualified technician. We strongly recommend that the technician succefully completes a suitable Gilson training program. Please contact your local Gilson distributor for details. Page Pipette Tips In accordance with the instructions given in its user’s guide, the pipette under test must be clean (refer to the decontamination procedure), correctly assembled (refer to the user’s guide), and fitted with a new Gilson tip before starting the Verification Procedure. Because the quality of the tip used is a significant factor in ensuring that a pipette performs to specifications, tip selection is specially important in verification procedure. For example, all models of Pipetman are calibrated at the factory using Gilson Diamond tips, which are of the highest quality. Therefore, for Pipetman, you must only use the Gilson Diamond tips when carrying out the gravimetric test to have the best performance and results. Pipette model Volume range Tips P2, P2N, U2 P10, P10N, U10 C10, C8x10, C12x10 0.2 µL to 2 µL D10, DL10 DF10, DFL10 1.0 µL to 10 µL 0.5 to 10 µL P20, P20N, U20 2 µL to 20 µL U8x20, U12x20 1 µL to 20 µL DL10 *: Not valid with filter tips D200. P100N 10 µL to 100 µL P100, U100, 20 µL to 100 µL C100 5 µL to 100 µL C8x100, C12x100 5 µL to 100 µL D200 P200 50 µL to 200 µL F2 to F200* 2 µL to 200 µL P200N, U200 20 µL to 200 µL P8x200 20 µL to 200 µL U8x300, U12x300 C300 20 µL to 300 µL D300 C8x300, C12x300 Filter Tips DF30 DF100 DF200 DF300 P1000N 100 µL to 1000 µL P1000, U1000 200 µL to 1000 µL F250 to F1000 250 µL to 1000 µL D1000 DF1000 C1200 100 µL to 1200 µL C8x1200, C12x1200 D1200 DF1200 P5000, U5000 1 mL to 5 mL F5000 5 mL C5000 0.5 mL to 5 mL D5000 P10ml, U10ml, C10ml D10ml 1 mL to 10 mL Page Test Equipment To ensure the integrity of the Verification Procedure, all of the measuring instruments: balances, hygrometer and thermometers should be checked regularly. Balance Information on suitable balances (some of which have more than one sensitivity range) is available from the International Organization of Legal Metrology (OIML). Appropriate balances, conforming to OIML R76-1, should be used. Balances should be serviced, calibrated and certified by qualified technicians using weights traceable to an internationally recognized authority (OIML). Nominal Volume (µL) Display (mg) Balance Sensitivity < 100 100 µL to 1000 > 1000 0.001 0.01 0.1 10-6g 10-5g 10-4g Note: These requirements are more rigorous than those specified in ISO 8655-6, Table 1. The sensitivity of the balance chosen must be consistent with the accuracy required, which is one tenth of the deviation to be assessed. For Pipetman (all models) and Microman, select the sensitivity according to the pipette’s nominal volume (see table). For Distriman, test volumes are specified for each DistriTip model according to specific aliquot volumes (choose the sensitivity accordingly). The table on which the balance is placed must be equipped with a marble surface plate that is independent of the perimeter, to avoid transmitting vibrations. For the same reason, the table must not be in contact with a wall. Avoid placing the balance near to a window or near to a door to avoid too long a response time for the balance and irregular evaporation caused by drafts or greenhouse effects. Thermometer, Hygrometer, and Barometer Use a calibrated thermometer to measure the water temperature at the beginning and at the end of each test series. Use a thermometer with a maximum uncertainty of measurement of 0.2 °C. The hygrometer shall have a standard maximum uncertainty of 10% and the barometer a standard uncertainty of less than or equal to 0.5 kPa. Page Test Equipment Lid Lid Pot B Pot A Ref: 95F10018 Ref: 544301006 Pot C Weighing Containers Special containers are used to receive water from the test pipette during weighing. Controlling evaporation during the gravimetric test is essential. To minimize evaporation, Gilson uses custom-designed cylindrical flat-bottomed weighing containers made of nonporous plastic. A Weighing Kit (ref: F144700) consisting of four sizes of container (Pot A, B, C, and D), filters (ref: F123854) for cleaning them and tweezers (ref: F144706), is available from Gilson. Individual components of the kit are available as spares. When the volumes to be tested are less than 200 µL, Gilson uses weighing containers equipped with lids (see below). Pot A container and lid, both of which must be manipulated using tweezers to avoid handwarming, for volumes up to 20 µL. Pot B container and lid for volumes from 20 to 200 µL. This container is fitted with P 5000 O-rings to avoid hand-warming. Ref: F1090303 Pot C container (50 mm x 35 mm) for volumes from 200 to 5000 µL. Pot D Pot D container (70 mm x 50 mm) for volumes greater than 5000 µL. Water The liquid used for testing must be distilled or deionized water grade 3 (degassed) conforming to ISO3696 at room temperature. To avoid fluctuations in water temperature, use a large container as a water reservoir (Pot C or Pot D). The reservoir should contain sufficient water for all of the tests. Ref: F1490343 Page Verification Procedure Procedure Summary The Verification Procedure certifies both pipette accuracy and precision. Environmental conditions, test equipment, and other qualifications previously described in this document should be implemented to assure the validity of the test results. After pre-rinsing the tip, record ten individual weighings per selected volume. For variable volume pipettes, three volume settings are selected per pipette model based on the pipette’s useful volume range (nominal, approximately 50 % and minimum volume or 10 % of the nominal volume). For fixed volume pipettes (Pipetman F) only the nominal volume is used. 1 Set the pipette to its test volume (see table opposite). 2 Estimate the evaporation loss (for small volumes). 3 Perform the gravimetric test: record the weighings on the Verification Procedure Report. 4 Perform the calculations: record the results on the Verification Procedure Report. 5 Compare the results with the accuracy and precision specifications given in the user’s guide of the test pipette. Multichannel Pipettes Note: Gilson recommends simplifying the process by fitting a tip to the tested channel, only. Page 10 According to ISO 8655-6, “For the purpose of the test, each channel shall be regarded as a single channel and reported as such”. Consequently, for each of the following procedures: fill all channels simultaneously when aspirating the test liquid, then expel only the test liquid aspirated by the channel being tested into the weighing vessel. Verification Procedure Test Volumes Minimum Volume (µL) Pipetman Mid Range Volume (µL) Nominal Volume (µL) P2N 0.2 1 2 P2, U2 0.5 1 2 P10, U10, P10N 1 5 10 P20, U20, P20N 2 10 20 P100N 10 50 100 P100, U100 20 50 100 P200N 20 100 200 P200, U200 50 100 200 P1000N 100 500 1000 P1000, U1000 200 500 1000 P5000, U5000 1000 2500 5000 P10ml, U10ml 1000 5000 10000 Microman M10 1 5 10 M25 3 10 25 M50 20 - 50 M100 10 50 100 M250 50 125 250 M1000 100 500 1000 Distriman DistriTip Micro 2 5 DistriTip Mini 20 50 DistriTip Maxi 200 500 Pipetman Multichannel 8X200 20 10 100 1000 100 200 Pipetman Ultra Multichannel 8x20 2 12x20 2 8x300 30 12x300 30 10 20 10 20 150 300 150 300 Pipetman Concept C10 1 5 10 C100 10 50 100 C300 30 150 300 C1200 120 600 1200 C5000 500 2500 5000 C10ml 1000 5000 10000 Pipetman Concept Multichannel C8x10, C12x10 1 5 10 C8x100, C12x100 10 50 100 C8x300, C12x300 30 150 300 Page 11 Verification Procedure Estimating the Evaporation Rate (Mass Loss/Cycle) Weighing requires special care, for small volumes (< 50 µL, according to ISO 8655) use tweezers and weighing containers fitted with lids (Pot A). The goal is to minimize, control and quantify evaporation loss during the weighing cycle. Apart from the design of the weighing vessel, the test cycle time is important. Evaporation is estimated by performing a series of four simulated weighings, repeating the weighing cycle without dispensing to the weighing container. The total difference attributable to evaporation is calculated and divided by 4 to obtain an average. The rate is expressed in mg/cycle (or for one cycle the loss may be expressed in mg). For example, evaporation rates usually range for Pot A between 0.010 mg to 0.025 mg per weighing cycle. Recalculate the evaporation rate every 4 hours or whenever ambient conditions change (temperature, pressure, and humidity). 1 Add water to the weighing container until it is about one-third full. 2 Fit the weighing container with its lid and use tweezers to place it on the balance pan. 3 Using the pipette, aspirate a sample from the reservoir at the test volume setting. 4 Tare the balance and remove the weighing container from the balance pan. 5 Use tweezers to remove the lid. 6 Dispense the sample into the reservoir or to waste, not the weighing container. 7 Fit the weighing container with its lid and use tweezers to put it back on the balance pan. 8 Record the result e1. 9 Repeat steps 3 through 8 three times to obtain e2, e3, and e4. 10 Calculate the loss/cycle: e = | e1 + e2 + e3 + e4 | /4 (mg). 11 The evaporation loss/cycle e (mg) should be added to the mean mass before calculating the mean volume. Page 12 Verification Procedure Gravimetric Test Note *: ISO recommends that the orifice of the tip be immersed to between 2 mm and 3 mm below the surface of the water. However, you should first consult the user’s guide for the model of Gilson pipette that you are testing. According to ISO 8655-6: “The test shall be carried out in a draught-free room with stable environment.” 1 Place distilled or deionized water from the container in the weighing vessel to a depth of at least 3 mm.* (Refit lid for Pot A and B.) 2 Record the test conditions (ambient and water temperature, relative humidity, barometric pressure). 3 Select the test volume of your variable-volume piston pipette. 4 Fit the tip or capillary/piston assembly to the pipette (the manufacturer specifications are valid only when test executed with the manufacturers tips). 5 Wet pipette tip five times to reach equilibrium in the dead air volume (not needed for Distriman and Microman), but do not take into account for calculations. One test cycle should take less than 1 min. A consistent rhythm during weighing operation should be maintained. Repeat these steps 6 Change tip. 7 Pre-wet the tip once. 8 Pipette the test volume. 9 Determine tare mass (reset balance). 10 Remove the lid if needed (using the tweezers for pot A) 11 Open balance door, retrieve weighing container, deliver sample, refit its lid, if needed, using the tweezers, replace on the balance and close the door. 12 After allowing display to stabilize and record the mass. 13 Repeat the test cycle until ten measurements have been recorded as a series of masses m1 to m10. 14 For sample below or equal to 50µl, estimate evaporation loss by repeating steps 8 to 10 exactly as a normal sample weighing but without actually adding any sample to the weighing container. Record absolute value (ei) and repeat several (m) times. 15 Record the test conditions (ambient temperature, relative humidity, barometric pressure). Check that values are still within recommended limits. Page 13 Calculations Formulae t = (t1 + t2 )/2 1 Calculate the mean temperature (t) of the distilled water (rounded to the nearest 0.5 °C). B = (B1 + B2 )/2 2 Use the average barometric pressure (B) and mean temperature (t) to find the corresponding Z-factor from the table. Vi = Z (mi + e) Vi = individual volumes (µL) mi = individual masses (mg) e = evaporation loss (mg) 3 Multiply the weighings (mg), after any required correction for evaporation, by the Z-factor to obtain a series of volumes (µL). Z = Z-factor (µL/mg) V Σ V= n i=1 i n 4 Compute the mean volume from the series of volumes (µL). Vi = individual volumes V = mean volume n = number of weighings 5 Calculate the systematic error, which is the difference between the mean volume of actual measurements and the true value as specified by the volume setting of the pipette (selected volume). For fixed volume pipettes, replace Vs with Vo = nominal volume. es = V - Vs es = systematic error V = mean volume Vs = selected volume Accuracy may be expressed in µL or ... ... as a percentage. es = 100 (V - Vs )/Vs % S= Σ (V - V) n i=1 i 2 n-1 Vi = individual volumes (calculated as above) V = mean volume 6 Calculate the random error, which is the closeness of agreement between individual weighings. Quantifies the magnitude of scatter due to random error. Also known as Repeatability Standard Deviation (RSD). n = number of measurements s = repeatability standard deviation CV = 100 x s/ V Page 14 As a percentage, also known as coefficient of variation (CV). Calculations Z= Conversion factor (µL/mg) t = Average temperature (°C) B = Air pressure (kPa) Z-factor Z correction factors for distilled water as a function of test temperature and air pressure. B (kPa) 80 85 90 t (°C) 95 Z (µL/mg) 100 101.3 105 15.0 1.0017 1.0018 1.0019 1.0019 1.0020 1.0020 1.0020 15.5 1.0018 1.0019 1.0019 1.0020 1.0020 1.0021 1.0021 16.0 1.0019 1.0020 1.0020 1.0021 1.0021 1.0021 1.0022 16.5 1.0020 1.0020 1.0021 1.0021 1.0022 1.0022 1.0022 17.0 1.0021 1.0021 1.0022 1.0022 1.0023 1.0023 1.0023 17.5 1.0022 1.0022 1.0023 1.0023 1.0024 1.0024 1.0024 18.0 1.0022 1.0023 1.0023 1.0024 1.0025 1.0025 1.0025 18.5 1.0023 1.0024 1.0024 1.0025 1.0025 1.0026 1.0026 19.0 1.0024 1.0025 1.0025 1.0026 1.0026 1.0027 1.0027 19.5 1.0025 1.0026 1.0026 1.0027 1.0027 1.0028 1.0028 20.0 1.0026 1.0027 1.0027 1.0028 1.0028 1.0029 1.0029 20.5 1.0027 1.0028 1.0028 1.0029 1.0029 1.0030 1.0030 21.0 1.0028 1.0029 1.0029 1.0030 1.0031 1.0031 1.0031 21.5 1.0030 1.0030 1.0031 1.0031 1.0032 1.0032 1.0032 22.0 1.0031 1.0031 1.0032 1.0032 1.0033 1.0033 1.0033 22.5 1.0032 1.0032 1.0033 1.0033 1.0034 1.0034 1.0034 23.0 1.0033 1.0033 1.0034 1.0034 1.0035 1.0035 1.0036 23.5 1.0034 1.0035 1.0035 1.0036 1.0036 1.0036 1.0037 24.0 1.0035 1.0036 1.0036 1.0037 1.0037 1.0038 1.0038 24.5 1.0037 1.0037 1.0038 1.0038 1.0039 1.0039 1.0039 25.0 1.0038 1.0038 1.0039 1.0039 1.0040 1.0040 1.0040 25.5 1.0039 1.0040 1.0040 1.0041 1.0041 1.0041 1.0042 26.0 1.0040 1.0041 1.0041 1.0042 1.0042 1.0043 1.0043 26.5 1.0042 1.0042 1.0043 1.0043 1.0044 1.0044 1.0044 27.0 1.0043 1.0044 1.0044 1.0045 1.0045 1.0045 1.0046 27.5 1.0045 1.0045 1.0046 1.0046 1.0047 1.0047 1.0047 28.0 1.0046 1.0046 1.0047 1.0047 1.0048 1.0048 1.0048 28.5 1.0047 1.0048 1.0048 1.0049 1.0049 1.0050 1.0050 29.0 1.0049 1.0049 1.0050 1.0050 1.0051 1.0051 1.0051 29.5 1.0050 1.0051 1.0051 1.0052 1.0052 1.0052 1.0053 30.0 1.0052 1.0052 1.0053 1.0053 1.0054 1.0054 1.0054 Page 15 Sample of a Verification Procedure Report Pipetting System Information Pipette Serial number: Calibration date: Model: Manufacturer: Pipette owner: Number of channels: Status: Tips Tip model: Manufacturer: Batch number: Environmental Factors Temperature air (°C): Hygrometry (%): Z-factor: Pressure (hPa): Temperature water (°C): Evaporation (Yes/No): General Information Decontamination (Yes/No): Adjustment (Yes/No): Repair (Yes/No): Basis of adjustment (Ex/In): Statistics Summary Page 16 (µL) (µL) Vmin Vint Vnom Vmean,min Vmean,int Vmean,nom Systematic error Results Target Status Es (µL) Es (%) µL % Results Random error Target Status Results Random error Target Status SD (µL) CV (%) SD (µL) CV (%) or Channel # SD: Standard Deviation CV: Coefficient of Variation Es: Systematic Error Selected Mean Volume Volume Selected Mean Volume Volume (µL) (µL) 1 1 1 Vmin1 Vint1 Vnom1 Vmean,min1 Vmean,int1 Vmean,nom1 ... ... ... Vmin Vint Vnom Vmean,min Vmean,int Vmean,nom N N N VminN VintN VnomN Vmean,minN Vmean,intN Vmean,nomN Systematic error Results Target Status Es (µL) Es (%) µL % SD (µL) CV (%) SD (µL) CV (%) Sample of a Verification Procedure Report Calibration Details Selected Volume Minimum Volume (value µL) Mid Range Volume (value µL) Nominal Volume (value µL) 1 2 3 4 ... 10 Va1 Vb1 Vc1 Va10 Vb10 Vc10 Mid Range Volume (value µL) Nominal Volume (value µL) Va1,1 Vb1,1 Vc1,1 Va1,10 Vb1,10 Vc1,10 Ch. # or Selected Minimum Volume Volume (value µL) 1 1 2 3 4 ... 10 i 1 2 3 4 ... 10 Vai,1 Vbi,1 Vci,1 Vai,10 Vbi,10 Vci,10 N 1 2 3 4 ... 10 VaN,1 VbN,1 VcN,1 VaN,10 VbN,10 VcN,10 Page 17 Appendix Pipetman P / Neo Here are comparative tables for maximum permissible errors between ISO 8655 and Gilson. ISO 8655 maximum permissible errors are very wide, so as to have a conformity-basis for all pipettes. At Gilson our knowledge and kno-how allows us to be more stringent, which means the best pipette-performance. Model Volume (Reference) (µL) Pipetman Ultra Only for Pipetman Neo. Except for Pipetman Neo. Maximum Permissible Errors Gilson ISO 8655 Systematic Random error (µL) error (µL) Systematic Random error (µL) error (µL) P2 (F144801) Min 0.2 ± 0.024 P2N (F144561) 0.5 ± 0.025 U2 (F21021) Max. 2 ± 0.030 0.012 0.012 0.014 ± 0.08 ± 0.08 ± 0.08 0.04 0.04 0.04 P10 (F144802) Min. 1 ± 0.025 P10N (F144562) 5 ± 0.075 U10 (F21022) Max 10 ± 0.100 0.012 0.030 0.040 ± 0.12 ± 0.12 ± 0.12 0.08 0.08 0.08 P20 (F123600) Min. 2 P20N (F144563) 5 U20 (F21023) 10 Max. 20 ± 0.10 ± 0.10 ± 0.10 ± 0.20 0.03 0.04 0.05 0.06 ± 0.20 ± 0.20 ± 0.20 ± 0.20 0.10 0.10 0.10 0.10 P100 (F123615) Min. 10 P100N (F144564) 20 U100 (F21024) 50 Max. 100 ± 0.35 ± 0.35 ± 0.40 ± 0.80 0.10 0.10 0.12 0.15 ± 0.80 ± 0.80 ± 0.80 ± 0.80 0.30 0.30 0.30 0.30 P200 (F123601) Min. 20 P200N (F144565) 50 U200 (F21025) 100 Max. 200 ± 0.50 ± 0.50 ± 0.80 ± 1.60 0.20 0.20 0.25 0.30 ± 1.60 ± 1.60 ± 1.60 ± 1.60 0.60 0.60 0.60 0.60 P1000 (F123602) Min. 100 P1000N (F144566) 200 U1000 (F21026) 500 Max. 1000 ± 3 ± 3 ± 4 ±8 0.6 0.6 1.0 1.5 ±8 ±8 ±8 ±8 3.0 3.0 3.0 3.0 P5000 (F123603) Min. 1000 and 2000 U5000 (F21027) Max. 5000 ± 12 ± 12 ± 30 3 5 8 ± 40 ± 40 ± 40 15 15 15 P10ml (F161201) Min. 1 mL and 2 mL U10ml (F21028) 5 mL Max.10 mL ± 30 ± 30 ± 40 ± 60 6 6 10 16 ± 60 ± 60 ± 60 ± 60 30 30 30 30 Systematic error: expressed as the deviation of the mean of a tenfold measurement from the nominal or selected volume (see ISO 8655-6). Random error: expressed as the repeatability standard deviation of a tenfold measurement (see ISO 8655-6). Page 18 Appendix Pipetman Concept Model Volume (Reference) (µL) C10 (F31012) Min. 0.5 1 5 Max. 10 Pipetman F Maximum Permissible Errors Gilson ISO 8655 Systematic Random error (µL) error (µL) Systematic Random error (µL) error (µL) ± 0.040 ± 0.025 ± 0.060 ± 0.080 0.013 0.012 0.020 0.025 ± 0.120 ± 0.120 ± 0.120 ± 0.120 0.080 0.080 0.080 0.080 C100 (F31013) Min. 5 ± 0.35 10 ± 0.30 50 ± 0.38 Max. 100 ± 0.4 0.10 0.10 0.12 0.15 ± 0.8 ± 0.8 ± 0.8 ± 0.8 0.30 0.30 0.30 0.30 C300 (F31014) Min. 20 30 150 Max. 300 ± 0.80 ± 0.70 ± 0.90 ± 1.05 0.16 0.20 0.23 0.30 ± 4.00 ± 4.00 ± 4.00 ± 4.00 1.50 1.50 1.50 1.50 C1200 (F31015) Min. 100 120 600 Max. 1200 ± 2.5 ± 2.4 ± 3.6 ± 6.0 0.4 0.4 0.8 1.2 ± 16.0 ± 16.0 ± 16.0 ± 16.0 6.0 6.0 6.0 6.0 C5000 (F31016) Min. 500 2500 5000 ± 10 ± 15 ± 25 2 4 7 ± 40 ± 40 ± 40 15 15 15 C10ml (F31017) Min. 1000 5000 10000 ± 25 ± 30 ± 50 4 8 12 ± 60 ± 60 ± 60 30 30 30 Model Volume (Reference) (µL) Maximum Permissible Errors Gilson ISO 8655 Systematic Random error (µL) error (µL) Systematic Random error (µL) error (µL) F2 (F123770) 2 ± 0.08 0.03 ± 0.08 F5 (F123771) 5 ± 0.10 0.04 ± 0.125 0.075 F10 (F123772) 10 ± 0.10 0.05 ± 0.12 0.08 F20 (F123604) 20 ± 0.20 0.06 ± 0.20 0.10 F25 (F123775) 25 ± 0.25 0.07 ± 0.50 0.20 F50 (F123778) 50 ± 0.40 0.15 ± 0.50 0.20 F100 (F123784) 100 ± 0.80 0.25 ± 0.80 0.30 F200 (F123605) 200 ± 1.60 0.30 ± 1.60 0.60 F250 (F123787) 250 ± 3.00 0.75 ± 4.00 1.50 F300 (F123788) 300 ± 3.50 0.75 ± 4.00 1.50 F400 (F123789) 400 ± 3.60 0.80 ± 4.00 1.50 F500 (F123790) 500 ± 4.00 1.00 ± 4.00 1.50 F1000 (F123606) 1000 ± 8.00 1.30 ± 8.00 3.00 F5000 (F123607) 5000 ± 30.00 8.00 ± 40.00 15.00 0.04 Page 19 Appendix Microman Model Volume (Reference) (µL) Distriman Systematic Random error (µL) error (µL) Systematic Random error (µL) error (µL) Min. 1 ± 0.09 M10 (F148501) 5 ± 0.10 Max 10 ± 0.15 0.03 0.03 0.06 ± 0.20 ± 0.20 ± 0.20 0.10 0.10 0.10 Min. 3 ± 0.25 M25 (F148502) 10 ± 0.27 Max. 25 ± 0.30 0.08 0.08 0.10 ± 0.70 ± 0.70 ± 0.70 0.30 0.30 0.30 M50 (F148503) Min. 20 Max. 50 ± 0.34 ± 0.70 0.20 0.30 ± 0.70 ± 0.70 0.30 0.30 Min. 10 M100 (F148504) 50 Max. 100 ± 0.50 ± 0.75 ± 1.00 0.20 0.30 0.40 ± 1.50 ± 1.50 ± 1.50 0.60 0.60 0.60 Min. 50 M250 (F148505) 100 Max. 250 ± 1.50 ± 1.70 ± 2.50 0.30 0.30 0.50 ± 6.00 ± 6.00 ± 6.00 2.00 2.00 2.00 Min. 100 M1000 (F148506) 500 Max. 1000 ± 3.00 ± 5.00 ± 8.00 1.60 2.50 4.00 ± 12.00 ± 12.00 ± 12.00 4.00 4.00 4.00 DistriTips Volume Model (µL) (Reference) Maximum Permissible Errors Gilson ISO 8655 Systematic Random error (µL) error (µL) Systematic Random error (µL) error (µL) 125 µL Min. 2 ± 0.100 0.080 Micro (F164100) 5 ± 0.125 0.075 Micro ST (F164130) Max 10 ± 0.200 0.100 ± 0.20 ± 0.20 ± 0.20 0.10 0.10 0.10 1250 µL Min. 20 ± 0.80 Mini (F164110) 50 ± 1.00 Mini ST (F164140) Max. 100 ± 1.00 0.20 0.40 0.60 ± 1.50 ± 1.50 ± 1.50 0.60 0.60 0.60 12.5 mL Min. 200 ± 6.00 Maxi (F164120) 500 ± 7.50 Maxi ST (F164150) Max. 1000 ± 10.00 1.00 1.50 2.50 ± 12.00 ± 12.00 ± 12.00 4.00 4.00 4.00 ST means Sterilized. Page 20 Maximum Permissible Errors Gilson ISO 8655 Appendix Pipetman Ultra Multichannel Model Volume (Reference) (µL) Maximum Permissible Errors Gilson ISO 8655 Systematic Random error (µL) error (µL) Systematic Random error (µL) error (µL) 8x20 (F21040) Min. 1 and 2 12x20 (F21041) 10 Max 20 ± 0.10 ± 0.10 ± 0.20 ± 0.40 0.08 0.08 0.10 0.20 ± 0.40 ± 0.40 ± 0.40 ± 0.40 0.20 0.20 0.20 0.20 8x300 (F21042) Min. 20 and 30 12x300 (F21043) 150 Max. 300 ± 1.00 ± 1.00 ± 1.50 ± 3.00 0.35 0.35 0.60 1.00 ±8 ±8 ±8 ±8 3.00 3.00 3.00 3.00 Pipetman 8X200 Model Volume (Reference) (µL) 8x200 (F161004) Min. 20 50 100 Max. 200 Pipetman Concept Multichannel Model Volume (Reference) (µL) Maximum Permissible Errors Gilson ISO 8655 Systematic Random error (µL) error (µL) ± 0.50 ± 0.50 ± 1.00 ± 2.00 0.25 0.25 0.50 1.00 Systematic Random error (µL) error (µL) ± 3.20 ± 3.20 ± 3.20 ± 3.20 1.20 1.20 1.20 1.20 Maximum Permissible Errors Gilson ISO 8655 Systematic Random error (µL) error (µL) Systematic Random error (µL) error (µL) C8x10 (F31032) Min. 1 and 5 C12x10 (F31042) Max. 10 ± 0.04 ± 0.08 ± 0.10 0.02 0.04 0.06 ± 0.24 ± 0.24 ± 0.24 0.16 0.16 0.16 C8x100 (F31033) Min. 10 and 50 12x100 (F31043) Max. 100 ± 0.25 ± 0.50 ± 0.80 0.14 0.20 0.25 ± 1.60 ± 1.60 ± 1.60 0.60 0.60 0.60 C8x300 (F31034) Min. 30 and 150 C12x300 (F31044) 300 ± 1.00 ± 1.50 ± 2.40 0.18 0.38 0.60 ± 8.00 ± 8.00 ± 8.00 3.00 3.00 3.00 Page 21 Associated Documents Documents Page 22 Gilson Reference Pipetman Ultra User’s Guide Pipetman P User’s Guide Pipetman Neo Addendum Pipetman F User’s Guide Pipetman 8X200 User’s Guide Pipetman Ultra Multichannel User’s Guide Pipetman Concept User’s Guide Microman User’s Guide Distriman User’s Guide LT801441 LT801117 LT801511 LT801118 LT801236 LT801462 LT801489 LT801502 LT801285 Decontamination Procedure LT802288 Notes Page 23 World Wide Web: www.gilson.com &: sales@gilson.com, service@gilson.com, training@gilson.com World Headquarters Gilson, Inc. 3000 Parmenter Street, P.O. Box 620027, Middleton, WI 53562-0027, USA Telephone: (1) 800-445-7661 or (1) 608-836-1551 • Fax: (1) 608-831-4451 Gilson SAS 19 avenue des Entrepreneurs, B.P. 145 95400 Villiers-le-Bel, France Telephone: (33) 1-34-29-50-00 • Fax: (33) 1-34-29-50-20
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