DF1000 Verification Procedure For Accuracy And Precision

User Manual: DF1000

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Verication Procedure

for

Accuracy and Precision

(defined for Users to incorporate in SOPs)

In accordance with ISO8655 Standard

Procedure LT802292/F - © 2007 Gilson SAS All rights reserved August 2007

Standard Operating Procedure for Pipettes

Page 2

Contents

In this document the word “tip” is used in the generic sense, where tip

is the disposable part that must be used with the pipette: for Distriman

this means “DistriTip”, for Microman “capillary-piston” and for Pipetman

“Gilson Diamond Tip”.

Glossary 3

Introduction 4

Environmental Conditions 5

Technician 6

Pipette Operation 6

Training 6

Pipette Tips 7

Test Equipment 8

Balance 8

Thermometer, Hygrometer and Barometer 8

Weighing Containers 9

Water 9

Verification Procedure 10

Procedure Summary 10

Multichannel Pipettes 10

Test Volumes 11

Estimating the Evaporation Rate (Mass Loss/Cycle) 12

Gravimetric Test 13

Calculations

Formulae 14

Z-factor 15

Verification Procedure Report 16

Appendix 18

Associated Documents 22

Notes 23

Page 3

Glossary

adjustment manufacture of an apparatus within

appropriate tolerances, or the suppli-

er’s setting of the apparatus, ensur-

ing the metrological performance,

as specified in the applicable part of

ISO 8655.

calibration set of operations that establish the

relationship between the dispensed

volume and the corresponding

nominal or selected volume of the

apparatus.

maximum permissible error upper or lower permitted extreme

value for the deviation of the dis-

pensed volume from the nominal

volume or selected volume of a

piston-operated volumetric appa-

ratus.

systematic error difference between the dispensed

volume and the nominal volume or

selected volume of the piston-oper-

ated volumetric apparatus.

random error scatter of the dispensed volumes

around the mean of the dispensed

volumes.

uncertainty of measurement parameter, associated with the dis-

pensed volume, that characterizes

the dispersion of the volumes that

could reasonably be attributed to

the dispensed volume.

nominal volume volume specified by the manufac-

turer and used for identification

and for indication of the measuring

range.

Page 4

Introduction

This document describes a verification procedure for the following

Gilson pipettes: Pipetman® Ultra (Single and Multichannel), Pipetman®

Concept (Single and Multichannel), Pipetman® P, Pipetman® Neo,

Pipetman® F, Pipetman® 8X200, Microman® and Distriman®.

The procedure is for verifying pipette performance using gravimetric

tests of repeated aspirate and dispense cycles with distilled water

(grade 3, ISO 3696), in controlled conditions. The test conditions and

methods described herein are fully compatible with ISO 8655 and are

often stricter than those specified in the international standard, as

are the expected results for maximum permissible errors, which are

tabulated in the Appendix.

So, adherence to this procedure assures conformity to Gilson’s

specifications for accuracy (systematic error) and precision (random

error) and to ISO requirements.

The procedure, which for small volumes includes a correction for

evaporation loss, evaluates the total system of pipetting: pipette, tip,

and operator. Therefore the procedure must be carried out by suitably

qualified and trained technicians. In calculating the volumes from

balance readings, corrections are made for the temperature and air

pressure when the test was made (Z-factor, refer to page 15).

Although the document does not directly concern itself with other

tests performed by the user, the method and calculations described

herein may be applied in other tests, outside the scope of this docu-

ment. Users shall establish a regular testing routine at least once a

year for their piston pipettes according to: accuracy and precision

requirements, frequency of use, number of operators using the pipette,

number of operations on each occasion of use and the nature of the

liquids being dispensed.

In the case of Pipetman Ultra and Pipetman Concept, the number of

cycles can be the basis of your regular testing routine.

Page 5

Environmental Conditions

The test shall be carried out in a draught-free room with a stable

environment.

The test room (laboratory) shall have humidity and temperature

control so that the atmospheric conditions of the environment

where the procedure will take place and the temperature of the

equipment used are stable and homogeneous before and during

the procedure.

The use of a chart recorder is recommended.

The temperature of the pipettes being verified and the distilled

water (grade 3, ISO 3696) used in the gravimetric test should have

stabilized before the procedure commences.

The pipettes, water and test apparatus should have been placed

in the test room at least 2 hours before starting the tests.

Ideally, verification takes place under the following conditions:

1) Temperature (t)

ISO 8655 recommends that the gravimetric tests take place where

the ambient and water temperature (t) are stable (± 0.5 °C) between

15 °C and 30 °C. Gilson recommends a range between 20 °C and 23

°C with a constant temperature (± 0.5 °C) between the beginning

and the end of gravimetric tests. It is recommended to put water

and pipettes at least 2 hours in the calibration room to reach an

equilibrium with the room conditions. Z-factor is used to convert

mass into volume according to temperature and pressure.

2) Relative humidity (RH)

ISO 8655 states that the RH must be greater than 50%. However,

Gilson recommends that a humidity range of between 50% and

75% be maintained throughout the verification procedure. In all

cases, the evaporation rate will be evaluated for volumes 50 µL.

3) Barometric pressure

Gilson’s tests should take place at 1013 ± 25 hPa. The barometric

pressure in the test room shall be recorded to the nearest 0.5 kPa.

Z-factor is used to convert mass into volume according to tem-

perature and pressure.

Page 6

Technician

Pipette Operation

Consistency of pipetting technique contributes significantly to the

reproducibility of the results of the Verification Procedure. Inexpe-

rienced technicians can cause substantial variations in apparent

pipette performance. For meaningful test results, the technicians

must be well-trained and qualified.

You should operate the pipette according to the instructions given

in the user’s guide of the pipette under test. Attention should be

given to maintain a steady rhythm when aspirating and dispensing

samples, speed and smoothness when pressing and releasing the

push-button, and tip immersion depth. The test cycle time shall be

kept to a minimum. It should not exceed 60 s.

Training

The Verification Procedure described in this document must be car-

ried out by a suitably qualified technician. We strongly recommend

that the technician succefully completes a suitable Gilson training

program. Please contact your local Gilson distributor for details.

Page 7

Pipette Tips

In accordance with the instructions given in its user’s guide, the pipette

under test must be clean (refer to the decontamination procedure),

correctly assembled (refer to the user’s guide), and fitted with a new

Gilson tip before starting the Verification Procedure.

Because the quality of the tip used is a significant factor in ensuring

that a pipette performs to specifications, tip selection is specially

important in verification procedure.

For example, all models of Pipetman are calibrated at the factory using

Gilson Diamond tips, which are of the highest quality. Therefore, for

Pipetman, you must only use the Gilson Diamond tips when carrying

out the gravimetric test to have the best performance and results.

DF30

D5000

DF1200D1200

100 µL to 1200 µL

D200

Pipette model Volume range Tips Filter Tips

P2, P2N, U2 0.2 µL to 2 µL

P10, P10N, U10 1.0 µL to 10 µL D10, DL10 DF10, DFL10

C10, C8x10, C12x10 0.5 to 10 µL

P20, P20N, U20 2 µL to 20 µL

U8x20, U12x20 1 µL to 20 µL DL10

P100N 10 µL to 100 µL

P100, U100, 20 µL to 100 µL

C100 5 µL to 100 µL DF100

C8x100, C12x100 5 µL to 100 µL

P200 50 µL to 200 µL

F2 to F200* 2 µL to 200 µL

P200N, U200 20 µL to 200 µL DF200

P8x200 20 µL to 200 µL

U8x300, U12x300

C300 20 µL to 300 µL D300 DF300

C8x300, C12x300

P1000N 100 µL to 1000 µL

P1000, U1000 200 µL to 1000 µL D1000 DF1000

F250 to F1000 250 µL to 1000 µL

C1200

C8x1200, C12x1200

P5000, U5000 1 mL to 5 mL

F5000 5 mL

C5000 0.5 mL to 5 mL

P10ml, U10ml, C10ml 1 mL to 10 mL D10ml

*: Not valid with filter tips D200.

Page 8

Test Equipment

To ensure the integrity of the Verification Procedure, all of the measur-

ing instruments: balances, hygrometer and thermometers should be

checked regularly.

Balance

Information on suitable balances (some of which have more than one

sensitivity range) is available from the International Organization of

Legal Metrology (OIML). Appropriate balances, conforming to OIML

R76-1, should be used. Balances should be serviced, calibrated and

certified by qualified technicians using weights traceable to an inter-

nationally recognized authority (OIML).

The sensitivity of the balance chosen must be consistent

with the accuracy required, which is one tenth of the devia-

tion to be assessed.

For Pipetman (all models) and Microman, select the

sensitivity according to the pipette’s nominal volume (see

table).

For Distriman, test volumes are specified for each DistriTip

model according to specific aliquot volumes (choose the

sensitivity accordingly).

The table on which the balance is placed must be equipped with a

marble surface plate that is independent of the perimeter, to avoid

transmitting vibrations. For the same reason, the table must not be

in contact with a wall. Avoid placing the balance near to a window or

near to a door to avoid too long a response time for the balance and

irregular evaporation caused by drafts or greenhouse effects.

Thermometer, Hygrometer, and Barometer

Use a calibrated thermometer to measure the water temperature at the

beginning and at the end of each test series. Use a thermometer with a

maximum uncertainty of measurement of 0.2 °C. The hygrometer shall

have a standard maximum uncertainty of 10% and the barometer a

standard uncertainty of less than or equal to 0.5 kPa.

Note: These requirements are more rigorous

than those specified in ISO 8655-6, Table 1.

Nominal Display Balance

Volume (µL) (mg) Sensitivity

< 100 0.001 10-6g

100 µL to 1000 0.01 10-5g

> 1000 0.1 10-4g

Page 9

Test Equipment

Weighing Containers

Special containers are used to receive water from the test pipette

during weighing. Controlling evaporation during the gravimetric test

is essential. To minimize evaporation, Gilson uses custom-designed

cylindrical flat-bottomed weighing containers made of nonporous

plastic.

A Weighing Kit (ref: F144700) consisting of four sizes of container (Pot

A, B, C, and D), filters (ref: F123854) for cleaning them and tweezers

(ref: F144706), is available from Gilson. Individual components of the

kit are available as spares.

When the volumes to be tested are less than 200 µL, Gilson uses weigh-

ing containers equipped with lids (see below).

Pot A container and lid, both of which must be manipulated using

tweezers to avoid handwarming, for volumes up to 20 µL.

Pot B container and lid for volumes from 20 to 200 µL. This container

is fitted with P 5000 O-rings to avoid hand-warming.

Pot C container (50 mm x 35 mm) for volumes from 200 to 5000 µL.

Pot D container (70 mm x 50 mm) for volumes greater than 5000 µL.

Water

The liquid used for testing must be distilled or deionized water grade

3 (degassed) conforming to ISO3696 at room temperature. To avoid

fluctuations in water temperature, use a large container as a water

reservoir (Pot C or Pot D). The reservoir should contain sufficient water

for all of the tests.

Pot A

Pot B

Pot C

Pot D

Ref: 95F10018

Ref: 544301006

Ref: F1090303

Ref: F1490343

Lid Lid

Page 10

Verication Procedure

Procedure Summary

The Verification Procedure certifies both pipette accuracy and

precision. Environmental conditions, test equipment, and other

qualifications previously described in this document should be

implemented to assure the validity of the test results.

After pre-rinsing the tip, record ten individual weighings per se-

lected volume. For variable volume pipettes, three volume settings

are selected per pipette model based on the pipette’s useful volume

range (nominal, approximately 50 % and minimum volume or 10

% of the nominal volume). For fixed volume pipettes (Pipetman F)

only the nominal volume is used.

1 Set the pipette to its test volume (see table opposite).

2 Estimate the evaporation loss (for small volumes).

3 Perform the gravimetric test: record the weighings on the

Verification Procedure Report.

4 Perform the calculations: record the results on the Verification

Procedure Report.

5 Compare the results with the accuracy and precision specifica-

tions given in the user’s guide of the test pipette.

Multichannel Pipettes

According to ISO 8655-6, “For the purpose of the test, each channel

shall be regarded as a single channel and reported as such”. Con-

sequently, for each of the following procedures: fill all channels

simultaneously when aspirating the test liquid, then expel only

the test liquid aspirated by the channel being tested into the

weighing vessel.

Note: Gilson recommends simplifying

the process by fitting a tip to the tested

channel, only.

Page 11

Verication Procedure

Test Volumes

Minimum Mid Range Nominal

Volume (µL) Volume (µL) Volume (µL)

Pipetman

P2N 0.2 1 2

P2, U2 0.5 1 2

P10, U10, P10N 1 5 10

P20, U20, P20N 2 10 20

P100N 10 50 100

P100, U100 20 50 100

P200N 20 100 200

P200, U200 50 100 200

P1000N 100 500 1000

P1000, U1000 200 500 1000

P5000, U5000 1000 2500 5000

P10ml, U10ml 1000 5000 10000

Microman

M10 1 5 10

M25 3 10 25

M50 20 - 50

M100 10 50 100

M250 50 125 250

M1000 100 500 1000

Distriman

DistriTip Micro 2 5 10

DistriTip Mini 20 50 100

DistriTip Maxi 200 500 1000

Pipetman Multichannel

8X200 20 100 200

Pipetman Ultra Multichannel

8x20 2 10 20

12x20 2 10 20

8x300 30 150 300

12x300 30 150 300

Pipetman Concept

C10 1 5 10

C100 10 50 100

C300 30 150 300

C1200 120 600 1200

C5000 500 2500 5000

C10ml 1000 5000 10000

Pipetman Concept Multichannel

C8x10, C12x10 1 5 10

C8x100, C12x100 10 50 100

C8x300, C12x300 30 150 300

Page 12

Verication Procedure

Estimating the Evaporation Rate (Mass Loss/Cycle)

Weighing requires special care, for small volumes (< 50 µL, accord-

ing to ISO 8655) use tweezers and weighing containers fitted with

lids (Pot A). The goal is to minimize, control and quantify evapora-

tion loss during the weighing cycle.

Apart from the design of the weighing vessel, the test cycle time

is important. Evaporation is estimated by performing a series of

four simulated weighings, repeating the weighing cycle without

dispensing to the weighing container. The total difference attrib-

utable to evaporation is calculated and divided by 4 to obtain an

average. The rate is expressed in mg/cycle (or for one cycle the loss

may be expressed in mg).

For example, evaporation rates usually range for Pot A between

0.010 mg to 0.025 mg per weighing cycle. Recalculate the evapora-

tion rate every 4 hours or whenever ambient conditions change

(temperature, pressure, and humidity).

1 Add water to the weighing container until it is about one-third

full.

2 Fit the weighing container with its lid and use tweezers to place

it on the balance pan.

3 Using the pipette, aspirate a sample from the reservoir at the

test volume setting.

4 Tare the balance and remove the weighing container from the

balance pan.

5 Use tweezers to remove the lid.

6 Dispense the sample into the reservoir or to waste, not the

weighing container.

7 Fit the weighing container with its lid and use tweezers to put

it back on the balance pan.

8 Record the result e1.

9 Repeat steps 3 through 8 three times to obtain e2, e3, and e4.

10 Calculate the loss/cycle: e = | e1 + e2 + e3 + e4 | /4 (mg).

11 The evaporation loss/cycle e (mg) should be added to the mean

mass before calculating the mean volume.

Page 13

Verication Procedure

Note *: ISO recommends that the

orifice of the tip be immersed to

between 2 mm and 3 mm below the

surface of the water. However, you

should first consult the user’s guide

for the model of Gilson pipette that

you are testing.

Gravimetric Test

According to ISO 8655-6: “The test shall be carried out in a draught-free

room with stable environment.”

1 Place distilled or deionized water from the container in the weigh-

ing vessel to a depth of at least 3 mm.* (Refit lid for Pot A and B.)

2 Record the test conditions (ambient and water temperature, rela-

tive humidity, barometric pressure).

3 Select the test volume of your variable-volume piston pipette.

4 Fit the tip or capillary/piston assembly to the pipette (the manu-

facturer specifications are valid only when test executed with the

manufacturers tips).

5 Wet pipette tip five times to reach equilibrium in the dead air

volume (not needed for Distriman and Microman), but do not

take into account for calculations.

One test cycle should take less than 1 min.

A consistent rhythm during weighing operation should be maintained.

6 Change tip.

7 Pre-wet the tip once.

8 Pipette the test volume.

9 Determine tare mass (reset balance).

10 Remove the lid if needed (using the tweezers for pot A)

11 Open balance door, retrieve weighing container, deliver sample,

refit its lid, if needed, using the tweezers, replace on the balance

and close the door.

12 After allowing display to stabilize and record the mass.

13 Repeat the test cycle until ten measurements have been recorded

as a series of masses m1 to m10.

14 For sample below or equal to 50µl, estimate evaporation loss by

repeating steps 8 to 10 exactly as a normal sample weighing but

without actually adding any sample to the weighing container.

Record absolute value (ei) and repeat several (m) times.

15 Record the test conditions (ambient temperature, relative

humidity, barometric pressure). Check that values are still within

recommended limits.

Repeat these steps

Page 14

Formulae

1 Calculate the mean temperature (t) of the distilled water (rounded

to the nearest 0.5 °C).

2 Use the average barometric pressure (B) and mean temperature (t)

to find the corresponding Z-factor from the table.

3 Multiply the weighings (mg), after any required correction for

evaporation, by the Z-factor to obtain a series of volumes (µL).

4 Compute the mean volume from the series of volumes (µL).

5 Calculate the systematic error, which is the difference between

the mean volume of actual measurements and the true value as

specified by the volume setting of the pipette (selected volume).

For fixed volume pipettes, replace Vs with Vo = nominal volume.

Accuracy may be expressed in µL or ...

... as a percentage.

6 Calculate the random error, which is the closeness of agreement

between individual weighings. Quantifies the magnitude of scatter

due to random error. Also known as Repeatability Standard Devia-

tion (RSD).

As a percentage, also known as coefficient of variation (CV).

Calculations

CV = 100 x s/ V

B = (B1 + B2 )/2

t = (t1 + t2 )/2

Vi

= individual volumes (µL)

Vi

= Z (mi + e)

mi

= individual masses (mg)

e = evaporation loss (mg)

Z = Z-factor (µL/mg)

V = i = 1

n

n

ΣVi

Vi = individual volumes

V = mean volume

n = number of weighings

es

= systematic error

Vs = selected volume

es

= V - Vs

V = mean volume

es

= 100 (V - Vs )/Vs %

i = 1

n - 1

n

Σ(Vi - V)2

S =



V = mean volume

n = number of measurements

s = repeatability standard

deviation

Vi

= individual volumes

(calculated as above)

Page 15

Z-factor

Z correction factors for distilled water as a function of test temperature

and air pressure.

Calculations

Z= Conversion factor (µL/mg)

t = Average temperature (°C)

B = Air pressure (kPa)

B (kPa) 80 85 90 95 100 101.3 105

t (°C) Z (µL/mg)

15.0 1.0017 1.0018 1.0019 1.0019 1.0020 1.0020 1.0020

15.5 1.0018 1.0019 1.0019 1.0020 1.0020 1.0021 1.0021

16.0 1.0019 1.0020 1.0020 1.0021 1.0021 1.0021 1.0022

16.5 1.0020 1.0020 1.0021 1.0021 1.0022 1.0022 1.0022

17.0 1.0021 1.0021 1.0022 1.0022 1.0023 1.0023 1.0023

17.5 1.0022 1.0022 1.0023 1.0023 1.0024 1.0024 1.0024

18.0 1.0022 1.0023 1.0023 1.0024 1.0025 1.0025 1.0025

18.5 1.0023 1.0024 1.0024 1.0025 1.0025 1.0026 1.0026

19.0 1.0024 1.0025 1.0025 1.0026 1.0026 1.0027 1.0027

19.5 1.0025 1.0026 1.0026 1.0027 1.0027 1.0028 1.0028

20.0 1.0026 1.0027 1.0027 1.0028 1.0028 1.0029 1.0029

20.5 1.0027 1.0028 1.0028 1.0029 1.0029 1.0030 1.0030

21.0 1.0028 1.0029 1.0029 1.0030 1.0031 1.0031 1.0031

21.5 1.0030 1.0030 1.0031 1.0031 1.0032 1.0032 1.0032

22.0 1.0031 1.0031 1.0032 1.0032 1.0033 1.0033 1.0033

22.5 1.0032 1.0032 1.0033 1.0033 1.0034 1.0034 1.0034

23.0 1.0033 1.0033 1.0034 1.0034 1.0035 1.0035 1.0036

23.5 1.0034 1.0035 1.0035 1.0036 1.0036 1.0036 1.0037

24.0 1.0035 1.0036 1.0036 1.0037 1.0037 1.0038 1.0038

24.5 1.0037 1.0037 1.0038 1.0038 1.0039 1.0039 1.0039

25.0 1.0038 1.0038 1.0039 1.0039 1.0040 1.0040 1.0040

25.5 1.0039 1.0040 1.0040 1.0041 1.0041 1.0041 1.0042

26.0 1.0040 1.0041 1.0041 1.0042 1.0042 1.0043 1.0043

26.5 1.0042 1.0042 1.0043 1.0043 1.0044 1.0044 1.0044

27.0 1.0043 1.0044 1.0044 1.0045 1.0045 1.0045 1.0046

27.5 1.0045 1.0045 1.0046 1.0046 1.0047 1.0047 1.0047

28.0 1.0046 1.0046 1.0047 1.0047 1.0048 1.0048 1.0048

28.5 1.0047 1.0048 1.0048 1.0049 1.0049 1.0050 1.0050

29.0 1.0049 1.0049 1.0050 1.0050 1.0051 1.0051 1.0051

29.5 1.0050 1.0051 1.0051 1.0052 1.0052 1.0052 1.0053

30.0 1.0052 1.0052 1.0053 1.0053 1.0054 1.0054 1.0054

Page 16

Sample of a Verication Procedure Report

Pipetting System Information

Selected Mean Systematic error Random error

Volume Volume Results Target Status Results Target Status

(µL) (µL) Es (µL) Es (%) µL % SD (µL) CV (%) SD (µL) CV (%)

Vmin Vmean,min

Vint Vmean,int

Vnom Vmean,nom

Statistics Summary

Serial number: Calibration date:

Model: Manufacturer:

Pipette owner:

Number of channels: Status:

Pipette

Tip model: Manufacturer: Batch number:

Tips

Environmental Factors

Temperature air (°C): Pressure (hPa):

Hygrometry (%): Temperature water (°C):

Z-factor: Evaporation (Yes/No):

General Information

Decontamination (Yes/No): Repair (Yes/No):

Adjustment (Yes/No): Basis of adjustment (Ex/In):

SD: Standard Deviation

CV: Coefficient of Variation

Es: Systematic Error

Selected Mean Systematic error Random error

Volume Volume Results Target Status Results Target Status

(µL) (µL) Es (µL) Es (%) µL % SD (µL) CV (%) SD (µL) CV (%)

1 Vmin1 Vmean,min1

1 Vint1 Vmean,int1

1 Vnom1 Vmean,nom1

... Vmin Vmean,min

... Vint Vmean,int

... Vnom Vmean,nom

N VminN Vmean,minN

N VintN Vmean,intN

N VnomN Vmean,nomN

Channel #

or

Page 17

Calibration Details

Sample of a Verication Procedure Report

Selected Minimum Volume Mid Range Volume Nominal Volume

Volume (value µL) (value µL) (value µL)

1 Va1 Vb1 Vc1

2

3

4

...

10 Va10 Vb10 Vc10

or

Selected Minimum Volume Mid Range Volume Nominal Volume

Volume (value µL) (value µL) (value µL)

1 1 Va1,1 Vb1,1 Vc1,1

2

3

4

...

10 Va1,10 Vb1,10 Vc1,10

i 1 Vai,1 Vbi,1 Vci,1

2

3

4

...

10 Vai,10 Vbi,10 Vci,10

N 1 VaN,1 VbN,1 VcN,1

2

3

4

...

10 VaN,10 VbN,10 VcN,10

Ch. #

Page 18

Pipetman P / Neo

Pipetman Ultra

Here are comparative tables for maximum permissible errors between

ISO 8655 and Gilson. ISO 8655 maximum permissible errors are very

wide, so as to have a conformity-basis for all pipettes. At Gilson our

knowledge and kno-how allows us to be more stringent, which means

the best pipette-performance.

Appendix

Systematic error: expressed as the deviation of the mean of a tenfold measurement from the nominal or selected

volume (see ISO 8655-6).

Random error: expressed as the repeatability standard deviation of a tenfold measurement (see ISO 8655-6).

Maximum Permissible Errors

Model Volume Gilson ISO 8655

(Reference) (µL) Systematic Random Systematic Random

error (µL) error (µL) error (µL) error (µL)

P2 (F144801) Min 0.2 ± 0.024  0.012 ± 0.08  0.04

P2N (F144561) 0.5 ± 0.025  0.012 ± 0.08  0.04

U2 (F21021) Max. 2 ± 0.030  0.014 ± 0.08  0.04

P10 (F144802) Min. 1 ± 0.025  0.012 ± 0.12  0.08

P10N (F144562) 5 ± 0.075  0.030 ± 0.12  0.08

U10 (F21022) Max 10 ± 0.100  0.040 ± 0.12  0.08

P20 (F123600) Min. 2 ± 0.10  0.03 ± 0.20  0.10

P20N (F144563) 5 ± 0.10  0.04 ± 0.20  0.10

U20 (F21023) 10 ± 0.10  0.05 ± 0.20  0.10

Max. 20 ± 0.20  0.06 ± 0.20  0.10

P100 (F123615) Min. 10 ± 0.35  0.10 ± 0.80  0.30

P100N (F144564) 20 ± 0.35  0.10 ± 0.80  0.30

U100 (F21024) 50 ± 0.40  0.12 ± 0.80  0.30

Max. 100 ± 0.80  0.15 ± 0.80  0.30

P200 (F123601) Min. 20 ± 0.50  0.20 ± 1.60  0.60

P200N (F144565) 50 ± 0.50  0.20 ± 1.60  0.60

U200 (F21025) 100 ± 0.80  0.25 ± 1.60  0.60

Max. 200 ± 1.60  0.30 ± 1.60  0.60

P1000 (F123602) Min. 100 ± 3  0.6 ± 8  3.0

P1000N (F144566)

200 ± 3  0.6 ± 8  3.0

U1000 (F21026) 500 ± 4  1.0 ± 8  3.0

Max. 1000 ± 8  1.5 ± 8  3.0

P5000 (F123603) Min. 1000 ± 12  3 ± 40  15

and 2000 ± 12  5 ± 40  15

U5000 (F21027) Max. 5000 ± 30  8 ± 40  15

P10ml (F161201) Min. 1 mL ± 30  6 ± 60  30

and 2 mL ± 30  6 ± 60  30

U10ml (F21028) 5 mL ± 40  10 ± 60  30

Max. 10 mL ± 60  16 ± 60  30

Except for Pipetman Neo.

Only for Pipetman Neo.

Page 19

Appendix

Pipetman F Maximum Permissible Errors

Model Volume Gilson ISO 8655

(Reference) (µL) Systematic Random Systematic Random

error (µL) error (µL) error (µL) error (µL)

F2 (F123770) 2 ± 0.08  0.03 ± 0.08  0.04

F5 (F123771) 5 ± 0.10  0.04 ± 0.125  0.075

F10 (F123772) 10 ± 0.10  0.05 ± 0.12  0.08

F20 (F123604) 20 ± 0.20  0.06 ± 0.20  0.10

F25 (F123775) 25 ± 0.25  0.07 ± 0.50  0.20

F50 (F123778) 50 ± 0.40  0.15 ± 0.50  0.20

F100 (F123784) 100 ± 0.80  0.25 ± 0.80  0.30

F200 (F123605) 200 ± 1.60  0.30 ± 1.60  0.60

F250 (F123787) 250 ± 3.00  0.75 ± 4.00  1.50

F300 (F123788) 300 ± 3.50  0.75 ± 4.00  1.50

F400 (F123789) 400 ± 3.60  0.80 ± 4.00  1.50

F500 (F123790) 500 ± 4.00  1.00 ± 4.00  1.50

F1000 (F123606) 1000 ± 8.00  1.30 ± 8.00  3.00

F5000 (F123607) 5000 ± 30.00  8.00 ± 40.00  15.00

Pipetman Concept Maximum Permissible Errors

Model Volume Gilson ISO 8655

(Reference) (µL) Systematic Random Systematic Random

error (µL) error (µL) error (µL) error (µL)

C10 (F31012) Min. 0.5 ± 0.040  0.013 ± 0.120  0.080

1 ± 0.025  0.012 ± 0.120  0.080

5 ± 0.060  0.020 ± 0.120  0.080

Max. 10 ± 0.080  0.025 ± 0.120  0.080

C100 (F31013) Min. 5 ± 0.35  0.10 ± 0.8  0.30

10 ± 0.30  0.10 ± 0.8  0.30

50 ± 0.38  0.12 ± 0.8  0.30

Max. 100 ± 0.4  0.15 ± 0.8  0.30

C300 (F31014) Min. 20 ± 0.80  0.16 ± 4.00  1.50

30 ± 0.70  0.20 ± 4.00  1.50

150 ± 0.90  0.23 ± 4.00  1.50

Max. 300 ± 1.05  0.30 ± 4.00  1.50

C1200 (F31015) Min. 100 ± 2.5  0.4 ± 16.0  6.0

120 ± 2.4  0.4 ± 16.0  6.0

600 ± 3.6  0.8 ± 16.0  6.0

Max. 1200 ± 6.0  1.2 ± 16.0  6.0

C5000 (F31016) Min. 500 ± 10  2 ± 40  15

2500 ± 15  4 ± 40  15

5000 ± 25  7 ± 40  15

C10ml (F31017) Min. 1000 ± 25  4 ± 60  30

5000 ± 30  8 ± 60  30

10000 ± 50  12 ± 60  30

Page 20

Appendix

Microman Maximum Permissible Errors

Model Volume Gilson ISO 8655

(Reference) (µL) Systematic Random Systematic Random

error (µL) error (µL) error (µL) error (µL)

Min. 1 ± 0.09  0.03 ± 0.20  0.10

M10 (F148501) 5 ± 0.10  0.03 ± 0.20  0.10

Max 10 ± 0.15  0.06 ± 0.20  0.10

Min. 3 ± 0.25  0.08 ± 0.70  0.30

M25 (F148502) 10 ± 0.27  0.08 ± 0.70  0.30

Max. 25 ± 0.30  0.10 ± 0.70  0.30

M50 (F148503) Min. 20 ± 0.34  0.20 ± 0.70  0.30

Max. 50 ± 0.70  0.30 ± 0.70  0.30

Min. 10 ± 0.50  0.20 ± 1.50  0.60

M100 (F148504) 50 ± 0.75  0.30 ± 1.50  0.60

Max. 100 ± 1.00  0.40 ± 1.50  0.60

Min. 50 ± 1.50  0.30 ± 6.00  2.00

M250 (F148505) 100 ± 1.70  0.30 ± 6.00  2.00

Max. 250 ± 2.50  0.50 ± 6.00  2.00

Min. 100 ± 3.00  1.60 ± 12.00  4.00

M1000 (F148506) 500 ± 5.00  2.50 ± 12.00  4.00

Max. 1000 ± 8.00  4.00 ± 12.00  4.00

Distriman Maximum Permissible Errors

DistriTips Volume Gilson ISO 8655

Model (µL) Systematic Random Systematic Random

(Reference) error (µL) error (µL) error (µL) error (µL)

125 µL Min. 2 ± 0.100  0.080 ± 0.20  0.10

Micro (F164100) 5 ± 0.125  0.075 ± 0.20  0.10

Micro ST (F164130) Max 10 ± 0.200  0.100 ± 0.20  0.10

1250 µL Min. 20 ± 0.80  0.20 ± 1.50  0.60

Mini (F164110) 50 ± 1.00  0.40 ± 1.50  0.60

Mini ST (F164140) Max. 100 ± 1.00  0.60 ± 1.50  0.60

12.5 mL Min. 200 ± 6.00  1.00 ± 12.00  4.00

Maxi (F164120) 500 ± 7.50  1.50 ± 12.00  4.00

Maxi ST (F164150) Max. 1000 ± 10.00  2.50 ± 12.00  4.00

ST means Sterilized.

Page 21

Pipetman Ultra

Multichannel

Appendix

Pipetman 8X200

Maximum Permissible Errors

Model Volume Gilson ISO 8655

(Reference) (µL) Systematic Random Systematic Random

error (µL) error (µL) error (µL) error (µL)

8x20 (F21040) Min. 1 ± 0.10  0.08 ± 0.40  0.20

and 2 ± 0.10  0.08 ± 0.40  0.20

12x20 (F21041) 10 ± 0.20  0.10 ± 0.40  0.20

Max 20 ± 0.40  0.20 ± 0.40  0.20

8x300 (F21042) Min. 20 ± 1.00  0.35 ± 8  3.00

and 30 ± 1.00  0.35 ± 8  3.00

12x300 (F21043) 150 ± 1.50  0.60 ± 8  3.00

Max. 300 ± 3.00  1.00 ± 8  3.00

Maximum Permissible Errors

Model Volume Gilson ISO 8655

(Reference) (µL) Systematic Random Systematic Random

error (µL) error (µL) error (µL) error (µL)

8x200 (F161004) Min. 20 ± 0.50  0.25 ± 3.20  1.20

50 ± 0.50  0.25 ± 3.20  1.20

100 ± 1.00  0.50 ± 3.20  1.20

Max. 200 ± 2.00  1.00 ± 3.20  1.20

Pipetman Concept

Multichannel Maximum Permissible Errors

Model Volume Gilson ISO 8655

(Reference) (µL) Systematic Random Systematic Random

error (µL) error (µL) error (µL) error (µL)

C8x10 (F31032) Min. 1 ± 0.04  0.02 ± 0.24  0.16

and 5 ± 0.08  0.04 ± 0.24  0.16

C12x10 (F31042) Max. 10 ± 0.10  0.06 ± 0.24  0.16

C8x100 (F31033) Min. 10 ± 0.25  0.14 ± 1.60  0.60

and 50 ± 0.50  0.20 ± 1.60  0.60

12x100 (F31043) Max. 100 ± 0.80  0.25 ± 1.60  0.60

C8x300 (F31034) Min. 30 ± 1.00  0.18 ± 8.00  3.00

and 150 ± 1.50  0.38 ± 8.00  3.00

C12x300 (F31044) 300 ± 2.40  0.60 ± 8.00  3.00

Page 22

Associated Documents

Documents Gilson Reference

Pipetman Ultra User’s Guide LT801441

Pipetman P User’s Guide LT801117

Pipetman Neo Addendum LT801511

Pipetman F User’s Guide LT801118

Pipetman 8X200 User’s Guide LT801236

Pipetman Ultra Multichannel User’s Guide LT801462

Pipetman Concept User’s Guide LT801489

Microman User’s Guide LT801502

Distriman User’s Guide LT801285

Decontamination Procedure LT802288

Page 23

Notes

World Wide Web

: www.gilson.com

&

: sales@gilson.com, service@gilson.com, training@gilson.com

World Headquarters

Gilson, Inc.

3000 Parmenter Street, P.O. Box 620027, Middleton, WI 53562-0027, USA

Telephone: (1) 800-445-7661 or (1) 608-836-1551 • Fax: (1) 608-831-4451

Gilson SAS

19 avenue des Entrepreneurs, B.P. 145

95400 Villiers-le-Bel, France

Telephone: (33) 1-34-29-50-00 • Fax: (33) 1-34-29-50-20