LC 4000 0926 Print0
User Manual: LC-4000
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LC-4000 Series Integrated HPLC and UHPLC systems A quick brief - LC-4000 Series The LC-4000 Series is the latest in a long history of innovative HPLC systems developed by JASCO reaching all the way back to the start of commercial HPLC in the early 1970s. The concept of the integrated LC-4000 series HPLC provides key separation platforms at 50MPa, 70MPa and 130MPa which correspond to conventional HPLC, the increasingly popular Rapid Analysis (RHPLC) and sub 2um UHPLC, respectively. Each platform is supplied with a dedicated pump and autosampler matched to the operating pressure and all three platforms share common detectors optimized for high-speed 100Hz acquisition and the narrow peak shapes common to both RHPLC and UHPLC. Advances in Solvent Delivery For over two decades JA SCO analy tical HPLC pumps have employed an asymmetric twinpiston solvent deliver y system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure profiles than the 2 commonly used conventional twinpiston reciprocating design. In the LC-4000 series, SSQD has been re-developed, with a completely new solvent delivery mechanism ExReFT (Extremely Reliable Flow Technology) offering the highest stability in solvent delivery across the entire analytical flow rate range used in the PU-4180/85 RHPLC and PU-4280/85 UHPLC pump models. Pioneering Optical Design New Technologies for Method Development As a pioneer in optical spectroscopy, JASCO has access to optical designs that few can match. Adopting designs from some of our more powerful spectrometers we have developed a range of HPLC optical detectors with unrivalled performance, like the new class leading FP4020 fluorescence detector with S/N of over 2300:1, the latest high-speed refractive index detector with micro cell optimized for RHPLC and UHPLC and a completely revised circular dichroismdetector for chiral chromatography. With the range of column technologies becoming ever more diverse - the traditional 5um fully porous particle is finally losing ground to some of the newer technologies such as the new superficially porous solid core (Coreshell) and the wide variety of sub-2um particles, the requirements for method development are becoming more demanding. The LC-4000 series has a number of newly developed accessories to help you make better separations faster, with accessories such as, flexible and stackable column ovens in a range of sizes to accommodate analytical to preparative columns and multiple columns at every scale and with options for integrated 6 or 10 solvent and column selection valves. C h r o m N A V, t h e c r o s s - p l a t f o r m chromatography data system, is evolving too. Version 2.0 now offers many new features and a great new look to make working with the LC-4000 HPLC system an even better user experience. Compact and Easy to Use Despite the extra power delivered by the LC-4000 series HPLC, the standard footprint is only 300 mm wide, a standard system requires only a very small amount of bench space. For easy user-maintenance, all LC-4000 instruments feature front-access for replacing consumables such as pump components and light sources, even the autosampler consumables such as the syringe parts are easy to access. For those users who require front-panel control, the LC4000 series retains the popular keypad and display (with a backlit LED display as standard). Keypad control can still be configured for convenient override when under PC control. Each LC-4000 module is designed for easy maintenance. Front-panel access to light source and consumables Front panel control is included as standard The LC-4000 Series pump modules requires smaller bench space than ordinary HPLC pumps. An integrated solvent delivery system can be incorporated into a single pump module. 3 System Configurations The LC-4000 series HPLC analytical and preparative HPLC systems have a number of common features 300mm wide footprint Each system component is completely stackable into a modest height The system is fully integrated with complete system monitoring All user replaceable components are accessible through the front panel Vertical leak/drain system to channel away solvent Integrated earthquake securing option All detectors include 100Hz acquistion with interchangeable flowcells Conventional HPLC and RHPLC Analytical Systems up to 70MPa The LC-4000 Series 70MPa system has been designed to future-proof your HPLC requirements. The LC-4000 70MPa system can be used with conventional 3 and 5µm particle size analytical columns at typical lower pressures around 10 to 20MPa (1500 to 3000psi) and can also be used with smaller particles such as shorter length UHPLC columns and superficially porous (SPP) or Coreshell that require slightly higher pressure solvent delivery to provide optimum linear velocity through the column. UHPLC Analytical Systems at 130MPa Ten years after the new segment of the HPLC market, UHPLC has found a niche for those that need to run a large number of samplers or get results quickly. Pioneering column technology is pushing the particle size even smaller. The near 20,000 psi operation of the LC-4000 130MPa is designed to take advantage of the separation efficiency of the very small particles couple with longer and narrower columns. The LC-4000 130MPa system incorporates many of the same features as the 70MPa system, but with materials designed to withstand higher pressures. Preparative HPLC Systems The LC-4000 preparative HPLC systems includes three platform options Up to 20mL/min for columns up to 21.2mm ID Up to 50mL/min for columns up to 3cm ID Up to 120mL/min for columns up to 5cm ID Large column oven for multiple preparative columns and optional automatic column switching Preparative detector options including high flow short path length flow-cells Open-bed fraction collector with sample trays for tubes, plates or large containers ChromNAV-FC for fraction control using time, threshold or slope from up to 4 different signals Semi-prep HPLC LC-4000 system 4 LC-4000 Series Extensive Range of Modules The LC-4000 Series, enables multiple system design based on the following modules. This concept can be used to create many different system configurations to match your application. The extensive range of detectors based on JASCO's spectroscopy technology is a key factor in providing a solution to virtually any application. PUMPS HPLC, RHPLC PU-4185/80 PU-4185B UHPLC PU-4285 PU-4285B PREP PU-4086/87 PU-4086B DETECTORS UV/Vis visible UV-4070/75 PDA MD-4010/15/17 Refractive Index RI-4030/35 AUTOSAMPLERS Fluorescence FP-4020/25 Circular Dichroism CD-4095 Optical rotation OR-4090 COLUMN OVENS CO - 4 0 6 0 high capacity CO-4065 Prep RO-4068 Reaction oven CO- 4061/62 compact HPLC AS-4050/50i RHPLC AS-4150 UHPLC AS-4250 Preparative AS-4058 5 The LC-4000 Series next generation HPLC pumps with flexible expandability The LC-4000 series HPLC pumps include expansion bays that allow a simple pump module to be transformed into a powerful solvent delivery system. Accessory modules can be installed in the LC-4000 series pump for use with virtually any solvent delivery requirement. Upgrade and Expansion The unique expansion bays in the pump platform can be fitted with plug-in modules to quickly and easily transform a simple isocratic pumping system into a sophisticated multi-solvent delivery system by installing a second pump, degasser, quaternary gradient unit, solvent selection valve or dynamic mixer without increasing the footprint. Adding a second pump module enables an isocratic pump to be upgraded to a dual pump system for either binary gradient or two isocratic flows. PU-4180 Analytical pump platform A powerful configuration of the single-unit design is the quaternary gradient solvent delivery system including degasser, with a post-column reagent pump configured in a compact chassis only 300mm wide. Excellent flow rate repeatability PU-4180 Flow rate accuracy (1.0mL/min, n=10) Mobile phase: Flow Rate: Column: Column temerature: Detection wave length: Sample: Injection: Acetonitrile/Water (80/20) 1.0mL/min CrestPak C18S (4.6mmI.D. x 150mm, 5µm) 40 deg C 260nm, Response; 1sec Polycyclic aromatic hydrocarbon (10µg/mL each) (Naphthalene, Fluorene, Anthracene, Pyrene, Chrysene, Benzo[a]pyrene) 10µL Retention time reproducibility (n=10) PU-4185 Semi micro pump platform # 1 2 3 4 5 6 7 8 9 10 Average Standard diviation（n-1） CV%（n-1） Naphthalene 3.1400 3.1417 3.1417 3.1400 3.1400 3.1400 3.1416 3.1400 3.1400 3.1400 3.1405 0.001 0.025 Fluorene 3.9367 3.9683 3.9367 3.9367 3.9367 3.9367 3.9383 3.9367 3.9367 3.9367 3.9370 0.001 0.018 Anthracene 4.5000 4.5017 4.5000 4.5002 4.5000 4.5000 4.5016 4.5000 4.5000 4.5017 4.5005 0.001 0.017 Pyrene 5.6283 5.6317 5.6300 5.6283 5.6283 5.6300 5.6317 5.6300 5.6300 5.6301 5.6299 0.001 0.022 Chrysene 6.4934 6.4950 6.4933 6.4950 6.4950 6.4933 6.4967 6.4950 6.4949 6.4950 6.4947 0.001 0.016 Benzo[a]pyrene 9.4267 9.4300 9.4285 9.4283 9.4283 9.4283 9.4317 9.4317 9.4317 9.4317 9.4297 0.002 0.020 Eliminating pulsation 6 The problems caused by pressure variations in solvent delivery when switching between the two pump heads has been improved with a new design that precisely controls the plunger drive. The LC- 4 0 0 0 series analytical pumps offer flow rate precision with RSDs of better than 0.05% (difficult to achieve with commercially available pumps). The PU-4180 and 4185 pumps operate at pressures up to 70 MPa, designed mainly for use with RHPLC for coreshell columns or similar micro packing materials as well as working at pressures commonly used for conventional HPLC. The PU- 4280 and PU- 4285 UHPLC pumps provide precise flow at pressures up to 130 MPa; for columns with particle sizes of less than 2 µm. The LC-4000 series UHPLC system can also be used for rapid analysis HPLC (RHPLC) and conventional HPLC. Pump Configurations The PU-4180/85 and PU-4280/85 analytical pumps can be configured for both single pump gradient and multiple pump gradient operation. The single pump quaternary gradient module offers excellent gradient formation for up to four solvents. The newly designed 4-way solvent valve for switching the four solvents is synchronized to the operation of the pump motor control - variable cycle adaption optimizes delivery according to gradient resolution and flow rate. This highly efficient quaternary gradient mode can be applied to a wide flow-rate range from low to high flow rates. Two and three pump binary or ternary gradient modules with optional built-in degasser are available for fast cycle high-resolution gradient formation; the response time is superior to the single pump quaternary gradient system. PU-4185-Binary pump Two pump binary gradient Solvent Mixing Multi-pump gradient mixing is done using a variable speed dynamic mixer with a range of a small volume mixing chambers incorporating a micro-stirrer to provide the smallest possible dead volume, offering higher mixing efficiencies for all HPLC solvents. The optional in-line TERA Mixer is a turbulent-flow solvent mixer uniquely designed to offer high efficiency mixing with minimal dead volume. PU-4180 Quaternary gradient mixing performance (1.0mL/min, n=10) Solvent Degassing The in-line vacuum degasser module uses a new Teflon AF membrane with a hold-up volume of only 400µL (per solvent line); this offers very quick solvent replacement with minimal waste. Despite its small size, the new degasser offers more effective and stable degassing than larger previous generation models. A degasser for preparative-HPLC is also available. Mobile phase: A; Water, B; Acetonitrile Gradient: (A/B) (65/35) → (5/95) Linear Gradient Flow rate: 1.0mL/min Column: CrestPak C18S (4.6mmI.D. x 150mm, 5µm) Column temerature: 40 deg C Detection wave length: 254nm, Response; 1sec Sample: Alkylphenon samles 10µg/mL (Benzohenon 5µg/mL) (Acetanilide, Acetophenone, Propiophenone, Butyrophenone, Benzophenone, Valerophenone, Hexanophenone, Heptanophenone,Octanophenone) Injection: 10µL Retention time reproducibility (n=10) # 1 2 3 4 5 6 7 8 9 10 Average Standard diviation (n-1) CV% (n-1) Acetanilide 3.117 3.117 3.113 3.117 3.117 3.117 3.117 3.117 3.117 3.117 3.116 0.001 0.034 Acetophenone 5.617 5.617 5.617 5.617 5.617 5.620 5.620 5.617 5.617 5.620 5.618 0.002 0.029 Propiophenone 7.590 7.590 7.590 7.590 7.590 7.593 7.590 7.590 7.593 7.593 7.591 0.002 0.021 Butyrophenone 9.303 9.307 9.307 9.307 9.307 9.310 9.307 9.310 9.310 9.313 9.308 0.003 0.030 Benzophenone 9.950 9.950 9.950 9.953 9.953 9.953 9.953 9.953 9.957 9.957 9.953 0.002 0.025 Valerophenone 10.980 10.983 10.983 10.983 10.983 10.987 10.983 10.987 10.987 10.990 10.985 0.003 0.026 Hexanophemone 12.583 12.587 12.587 12.587 12.587 12.587 12.587 12.590 12.593 12.593 12.588 0.003 0.026 Heptanophenone 14.067 14.067 14.067 14.067 14.067 14.067 14.067 14.067 14.073 14.073 14.070 0.002 0.016 Octanophenone 15.430 15.433 15.433 15.433 15.433 15.433 15.433 15.433 15.437 15.437 15.434 0.002 0.012 PU-4180 (compact quaternary gradient pump with micro-volume degasser ) *Option Preparative and Semi-preparative Pumps The LC-4000 Preparative-HPLC System includes pumps at three scales. The PU-4086 pump offers flow rates up to 20 mL/min, the PU-4087 pump up to 50 mL/min and the PU-4088 up to 120mL/ min. Both single and multi-pump gradient modes can be used with the PU-4086 pump platform whilst the PU-4087 and PU-4088 are used in multi-pump mode only. The new MK2 technology used in the PU-4086 and PU-4087 motor driver control has been increased so that the maximum pressure can be used at higher flow-rates for all pump models. Solvents can be delivered at higher flow rates and higher pressures for use with longer preparative HPLC columns that generate more back pressure and for faster and more productive preparative separations. Dynamic mixers, solvent selection valves and the prep degasser can be installed in the expansion bays in both the PU-4086 and PU-4087 modules. PU-4086 semi prep pump Recycling inlet switching unit, Recycling valve unit (Option) 7 A comprehensive range of high performance optical detectors With JASCO’s long history of spectrometer development, you will find that each detector is well designed; these next generation detectors employ the latest optical and electronic technologies for high sensitivity, excellent stability and easy maintenance. MD-4010, MD-4015 and MD-4017 PDA Detectors The LC-4000 Series includes three PDA detec tor s. T he MD - 4 017 is a useful replacement for a single wavelength detector, the MD-4015 is the workhor se for UV- visible detection in the range 200 to 600nm and the MD-4010 is designed for high sensitivity and high spectral and time resolution. The MD-4010 PDA detector offers the highest detection sensitivity, equivalent to a single wavelength detec tor. Making use of a 1024 element photometric diode array, the measurement wavelength range is from 190nm to 90 0nm with a MD-4010 PDA resolution of 1nm and fast spectrum acquisition rate of up to 100 spectra/ sec. When used with the new temperature controlled SP t ype semi-micro flow cell the MD-4010 is well suited to the narrower peaks typically found with UHPLC or RHPLC separations. The MD-4010 is fitted with an internal mercury lamp for automatic wavelength calibration. The MD-4015 PDA detector is the routine detector for conventional HPLC, RHPLC and UHPLC. The sensitivity is almost equivalent to that of the MD-4010 but only covers the wavelength range from 200nm to 600nm using a 512 element PDA for 1nm resolution and with high speed 100Hz spectrum acquisition. The MD-4017 detector has a price-point comparable to a typical single wavelength detector and has been designed for the user only interested in the UV region from 200-400nm, the resolution is excellent at 1nm and the maximum spectrum acquisition is 20 spectra per second (20Hz), useful for peak widths as small as 1 to 2 seconds. ChromNAV PDA data processing included as standard Front panel access for easy lamp replacement When used with ChromNAV 2.0 CDS the PDA detector offers multi-functional analysis with a variety of functions such as spectral analysis, library search for peak identification, peak purity and multi-wavelength quantitation. UV- 4070/4075UV-Visible Detectors Using the advanced CzernyTu r n e r m o n o c h r o m a t o r d e s i g n with optimization of the optical sys tem, baseline change due to refrac tive index is minimized. Baseline fluctuation from changes in environmental temperature is reduced with a temperature controlled cell, baseline drift caused by temperature fluctuation in the light source is also reduced with a temperature controlled light source. The result is excellent stability with UV-4070 UV Visible UV spectra auto-scan Automatic spectral scannning can be controlled using ChromNAV, when a peak exceeds a set threshold a scan is executed automatically. 0.24 220000 200000 Intensity[µV] the noise level +/- 0.2x10 -5 AU and drift 1x10 -4 AU/h. Both detectors offer simultaneous dual-wavelength data acquisition and spectral scanning. Us er maintenan c e c an b e d o n e without removing the module from the system. Front-panel access is used for light source replacement and automatic wavelength calibration can be carried out using a mercury lamp. Both detectors include high speed data acquisition at up to 100Hz and can be used for conventional HPLC with the standard flow cell and for UHPLC/RHPLC when used with the SP type semi-micro cell. 0.2 Abs 0.1 100000 Threshold 50000 0 -0.01 Threshold 10000 200 400 600 800 900 Wavelength[nm] 8 Spectral measurement view 0 0.0 0.2 0.4 0.6 0.8 Retention Time[min] 10.0 12.0 14.0 UV detector Auto scan parameters Fluorescence detector FP-4020/4025 The FP-4020 fluorescence detector has been designed for the pursuit of extreme sensitivity. Re-designing the optics and electronics from the ground up, as well as removing the effects of ambient temperature with a new temperatureregulated cell body has led to the highest sensitivity with stable detection. The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a giant leap in sensitivity for the FP-4020 to achieve a signal noise ratio of 2300:1 or higher (based on the water Raman peak). The FP-4025 is an excellent routine fluorescence detector that has a slightly lower specification with a water Raman S/N of 1400:1 . Both detectors can be used for simultaneous detection at two wavelength pairs; excitation and emission wavelengths can be changed during the acquisition using a time program and automatic spectra scanning can be triggered manually, by time or automatically by threshold. A built-in mercury lamp enables automatic wavelength correction and the front-access design allows easy replacement of the flow cell assembly and light source. FP-4020 Fluorescence 26000 20000 Intensity[µV] Anthracene 1.4 fg (S/N=3) (Anthracene 100 fg Injection) 10000 0.0 1.0 2.0 0 3.0 4.0 5.0 Retention Time[min] High sensitivity measurement of Anthracene Dual wavelength simultaneous measurement Refractive Index detector RI-4030/4035 RI-4030 Refractive Index The Refractive Index detectors include the optical system mounted in a precisely temperature-regulated housing to eliminate the influence of changes in ambient temperature. The RI-4030 and 4035 detectors are fully stable just one hour after powering on. The RI-4030 can be used for both analytical and preparative scales. In preparative mode the maximum flow rate is 120 ml/min. The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with semi-micro GPC analysis using GPC columns with a fine pore size. The 100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC separations. The LED light source in both detectors has a long life time (over 10,000 hours) for low maintenance operation. Both the RI-4030 and RI-4035 detectors can be fitted with an optional pressure release safety-valve to prevent damage to the flow cell in the event blockage. Circular Dichroism detector CD-4095 CD-4095 Circular Dichroism The CD-4095 is a unique HPLC detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeter. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column. The CD-4095 circular dichroism detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring the positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated, a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. The ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signal. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold. Chiral detector OR-4090 OR-4090 Optical Rotation The OR-4090 Chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds with no absorption. The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing a strong output in the UV/Visible region where the largest optical rotations offer the highest sensitivity. The wide wavelength range from 350-900nm also allows measurement of a wide variety of samples. One of the key benefits of optical rotation detection is that it is unaffected by changes in solvent and can be used with gradient elution, unlike an RI detector. 9 Autosamplers - AS-4050/4050i/4150/4250/4058 The LC-4000 Series autosamplers provide a range of options to suit any sample injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC model or the AS-4058 preparative scale autosampler. The AS-4050 is a 60 sample autosampler for use with conventional HPLC with options for analytical and semi-preparative injection volumes. The AS-4150 and AS-4250 are high-capacity, high performance autosamplers designed for RHPLC (up to 70MPa)and UHPLC (up to 130MPa), respectively, with a minimum injection cycle-time of only 30 seconds. Peltier cooling is available for all autosamplers and light protection for photolabile samples is available on the higher level models. AS-4050 60 sample HPLC autosampler Pre-injection sample preparation is available on the high performance autosamplers with programmable options for sample dilution and pre-column derivitization. The injection mode can be switched between full loop injection and partial loop injections. Up to 5 different washing solvents can be used to achieve excellent carry-over at less than 0.005%. An optional degasser can be installed for degassing up to 5 flushing solvents. The AS-4058 is the LC-4000 Series largest volume autosampler with injection volumes up to 5mL as standard (up to 10mL as an option). The AS-4058 is typically used in combination with either a PU-4087 or PU-4088 pump for preparative separations. All LC-4000 series autosamplers share the same simple maintenance features, with consumable parts such as injection ports, rotor seals, and loops accessible from the front panel for easy replacement. AS-4250 High capacity UHPLC autosampler Simple replacement of syringe in AS-4050 Designed for easy maintenance Metering Tube Air-drain Valve Waste Bottle Needle Flushing Port Solvent-stop Valve NC Air Pump IN 3way Valve 5 Sample Vial 4 Injection Port Sample loop NO Column 1 2 Injection Valve Flushing Solvent Metering Pump AS-4050/4150/4250/4058 flow diagram (loop injection) 10 To Detector 3 6 HPLC Pump Column Ovens The LC-4000 Series column ovens have been designed to provide excellent utility for a wide range of different user requirements. For simple requirements the stackable CO-4061 can be used with column lengths up to 200mm (or up to 300mm with an optional extension). CO-4061 For RHPLC and UHPLC the CO-4062 has capacity for up to six 150 mm length columns, with optional one or t wo internally mounted automatic switching valves for 2 or 6 column selection. For GPC and larger preparative columns, select either of the two high-capacity CO4060 or CO-4065 ovens. The CO-4060 can accommodate columns with lengths up to 300mm or larger ID preparative columns. The CO-4065 can accommodate up to 10 columns with lengths up to 300mm and a pair of column selection valves is included as standard. All column ovens have built- in electronic cooling for controlling column temperature at sub-ambient temperatures. The CO-4062 and CO-4061 block-heater type ovens can be fitted with an optional micro-volume pre-heating unit for preheating the mobile phase before the column to improve resolution and peak shape. The RO-4068 reaction oven is used for high temperature post-column sample processing with capacity for columns and reaction coils. CO-4060 600000 WIth Pre coulmn stabilizer — WIthout Pre coulmn stabilizer — Temp : 25 degree C Toluene X-Presspak VC18-W (3 mmI.D. x 50 mmL, 2 µm) Water / Acetonitrile (Gradient) 1.0 mL/min Gasoline Oxidization Products Eluent Flow Rate Sample 400000 Intensity Column 200000 Analysis conditions 0 0.0 0.4 0.8 1.2 1.6 2.0 600000 Temp : 40 degree C Toluene Intensity 400000 2.4 2.8 WIth Pre coulmn stabilizer — WIthout Pre coulmn stabilizer — Room Temp. Oven Temp. 25˚C 200000 21˚C 0 0.0 0.4 0.8 1.2 1.6 2.0 2.4 2.8 Efficiency comparison of stabilizer 40˚C Pre-Column Stabilizer Theoretical Peak Width tR(min) With Without With Without 56095 54747 46021 36402 0.019 0.019 0.020 0.023 1.893 1.887 1.799 1.851 Comparison of Toluene peaks UniFinePak HPLC Columns UniFinePak is a new type of column that uses a hybrid packing material. Compared with traditional packing materials that use silica as the base material, UniFinePak columns can be used with a wider pH range from alkali to acid. UniFinePak columns offer an extended analysis range due to its chemical strength and resilience to high pressure operation. The entire range of UniFinePak columns uses a common packing material in a range of particle sizes from semi-micro to analytical to semi preparative scales, which simplifies method development when scaling-up separations from analytical to semipreparative . Method Conversion Program Optional Preparative HPLC fraction collection kit The LC-4000 series preparative system fraction collection kit includes Fraction collector Flow divertor valve ChromNav FC fraction control program for ChromNAV 11 ChromNAV 2.0 The 'PowerTool' for HPLC Analysis ChromNAV 2.0 (and ChromNAV-CFR 2.0) are JASCO’s next generation CDS developed from the powerful and easy-to-use ChromNAV 1.0 with a host of exciting new features. With a customizable graphical-user-interface (GUI), the user can set-up the system to display only the functions necessary for their application. This latest intuitive GUI allows the user to quickly learn the operation and explore the extensive functionality for data processing. ChromNAV 2.0 is a universal CDS which can be used with any type of separation - HPLC, UHPLC, RHPLC, prep-HPLC, analytical SFC and prep-SFC. ChromNav can also satisfiy the demands of dedicated analysis and multi-purpose systems. Control method The pump flow rate and gradient profile display is flexible and can be overlaid with a chromatogram for adjusting gradient conditions. PDA analysis PDA data processing is included as standard. Data is displayed in 2D contour plot and 3D with simultaneous overlay of spectra and chromatograms.Extract chromatograms at single or multiple wavelengths for quantitation ChromNAV 2.0 offers powerful system control and data acquisition with options for 2D (or up to 4D) gradient control and multi-zone oven control with control of up to 3 column ovens for method development. Solvent and column selection control is increased to include up to 10 positions for both. During acquisition, the run-time for a chromatogram can be increased to capture later eluting peaks which may exceed the set run-time. 12 Previously acquired chromatograms can be overlaid for visual comparison with the data currently being acquired, as a quick indicator to t h e u s e r o f c u r r e nt s e p a r a t i o n performance. manually, by time or by threshold. A newly added feature of ChromNAV 2.0 is the automatic message that can be emailed to your PC or smartphone to give an update on the progress of the sequence. Data acquisition and analysis for a PDA detector are included as standard in ChromNAV. With the increasing requirements for Green Energy, the LC-4000 series has the facility not only to power off and save lamp life at the end of the run, but also a ‘soft-off’ function to automatically power off a module at the end of the sequence. The fluorescence and UV-visible detectors include spectral scanning as standard, which can be triggered ChromNav 2.0 standard features • • • • • • • Peak integration and peak identification Peak grouping Linear and non-linear quantitation 3D chromatogram analysis, Spectral analysis for UV-visible, Fluorescence and PDA detectors Style report generator User calculation All raw data is protected and saved; which can then be analyzed and re-analyzed, reported and saved with both the raw data and with any data processing from the user’s analysis. A comprehensive audit trail records the acquisition method along with a history of instrument performance indicators in each sample data file, which provides the user a snap-shot of the condition of the system during the run and can warn about requirements for impending maintenance. ChromNAV 2.0 Optional Applications • • • • ChromNAV CFR for 21 CFR Part 11 compliance and electronic registration of data GPC molecular weight dispersion Molecular weight dispersion of low molecular weight heparin FUMI theory (Function of Mutual Information) for theoretical precision analysis ChromNAV-GPC Molecular weight distribution program ( Option ) ChromNAV GPC/SEC calculation program for GPC analysis ChromNAV- FC Collection view window ( Option) ChromNAV-FC fraction collector control for the CHF122SC fraction collector ChromNAV-FC for preparative HPLC The optional ChromNAV-FC fraction collector control is available as part of the Prep-HPLC kit and provides ChromNav with the capability to control the LC-4000 Series preparative HPLC system for fraction collection. or circular dichroism. Fraction simulation is applied to an acquired chromatogram to optimize the fraction collection parameters and the graphical display can be used to locate peaks of interest in the fraction collector tray. ChromNAV-FC offers control for two different capacity fraction collectors, with sophisticated fractionation control with several methods for peak collection, such as time, threshold and slope, and can even be programmed to use data collected simultaneously from multiple detectors such as UV-visible, refractive index ChromNAV-FC also supports additional functions for prep-HPLC applications, including automatic multi-channel fractions, fraction repeat and fraction re-cycle. *1: ChromNAV CFR is an optional software package for ChromNAV. 13 Specifications Pump Model PU-4185 Flow rate setting range 0.001 ~ 4.0 mL/min Applicable flow rate range Maximum pressure Flow rate accuracy 0.05 ~ 3.0 mL/min (~ 70 MPa) ~ 4.0 mL/min (~ 40 MPa) PU-4180 PU-4285 0.001 ~ 10.0 mL/min 0.001 ~ 2.0 mL/min 0.5 ~ 6.0 mL/min (~ 70 MPa) 0.050 ~ 1.5 mL/min (~ 130 MPa) ~ 10.0 mL/min (~ 35 MPa) 1.501 ~ 2.0mL/min (~ 100 MPa) 70 MPa (~ 3.0 mL/min) 70 MPa ( ~ 6.0 mL/min) 130.0 MPa (~ 1.5 mL/min) 40 MPa (~ 4.0 mL/min) 35 MPa (~ 10.0 mL/min) 100.0 MPa (~ 2.0 mL/min) +/- 1% or +/- 2 µL/min, whichever is larger. +/- 1% or +/- 2 µL/min, whichever is larger. +/- 1% or +/- 2 µL/min, whichever is larger. (0.05 ~ 4.0 mL/min) (0.5 ~ 10.0 mL/min) (0.05 ~ 2.0 mL/min) 0.05% RSD or +/- 0.04min SD, whichever is larger. 0.05% RSD or +/- 0.04min SD, whichever is larger. 0.05 % RSD or +/- 0.04 min SD, whichever is Flow rate precision (0.05 ~ 4.0 mL/min) (0.5 ~ 5.0 mL/min) (measurement by chromatogram) (measurement by chromatogram) (measurement by chromatogram) Dimensions, weight 300(W) x 470(D) x 150(H) mm, 13 kg 300(W) x 470(D) x 150(H) mm, 13 kg 300(W) x 470(D) x 150(H) mm, 14.5 kg Power requirement AC 100 ~ 240 V, 50/60 Hz, 80 VA AC 100 ~ 240V, 50/60 Hz, 80 VA AC 100 ~ 240V, 50/60 Hz, 60 VA larger. (0.1 ~ 2.0 mL/min) (option) temp. range: 4 ~ 40ºC Gradient mode with low-pressure gradient unit Mixing accuracy Mixing precision Binary pump +/-0.6% (5 ~ 95%, 0.2 ~ 1.0 mL/min) +/-1.2% (5 ~ 95%, ~ 4.0 mL/min) +/-0.8% (5 ~ 95%, 0.5 ~ 5.0 mL/min) +/-0.6% (5 ~ 95%, 0.2 ~ 1.0 mL/min) +/-1.2% (5 ~ 95%, ~ 2.0 mL/min) 0.15% RSD or +/- 0.01min, whichever is larger. 0.25% RSD or +/-0.02min, whichever is larger. 0.15% RSD or +/-0.01min, whichever is larger. (0.2 ~ 4.0 mL/min) (0.5 ~ 5.0 mL/min) (0.2 ~ 2.0 mL/min) (measurement by chromatogram) (measurement by chromatogram) (measurement by chromatogram) PU-4185-Binary PU-4285-Binary Gradient mode Mixing accuracy +/-0.4% - (5 ~ 95%, 0.2 ~ 4.0 mL/min) 0.15% RSD or +/-0.01 min, whichever is larger. Mixing precision (5 ~ 95%, 0.2 ~ 2.0 mL/min) 0.15% RSD or +/-0.01 min, whichever is larger. (0.2 ~ 4.0 mL/min) - (0.2 ~ 2.0 mL/min) (measurement by chromatogram) (measurement by chromatogram) Dimensions, weight 300(W) x 470(D) x 150(H) mm, 19.5 kg - 300(W) x 470(D) x 150(H) mm, 22.5 kg Power requirement AC 100 ~ 240 V, 50/60 Hz, 130 VA - AC 100 ~ 240 V, 50/60 Hz, 110 VA Model PU-4086 Flow rate setting range 0.001 ~ 20.0 mL/min Applicable flow rate 0.5 ~ 20.0 mL/min range (0 ~ 50 MPa) PU-4087 0.01 ~ 50.0 mL/min 5.0 ~ 30.0 mL/min (~ 50 MPa) 30.01 ~ 40.0 mL/min (~ 40 MPa) 40.01 ~ 50.0 mL/min (~ 30 MPa) 50 MPa (~ 30.0 mL/min) Maximum pressure 50 MPa 40 MPa (~ 40.0 mL/min) 30 MPa (~ 50.0 mL/min) Flow rate accuracy +/- 1% or +/- 10 µL/min, whichever is larger. +/- 1% or +/- 50 µL/min, whichever is larger. (0.5 ~ 20.0 mL/min) (5.0 ~ 50.0 mL/min) 0.05% RSD or +/- 0.04min SD, whichever is larger. 0.1% RSD or +/- 0.04min SD, whichever is larger. Flow rate precision (0.5 ~ 20.0 mL/min) (5 ~ 40 mL/min) (measurement by chromatogram) (measurement by chromatogram) Dimensions, weight 300(W) x 470(D) x 150(H) mm, 14.5 kg 300(W) x 470(D) x 150(H) mm, 18 kg Power requirement AC 100 ~ 240 V, 50/60 Hz, 90 VA AC 100 ~ 240 V, 50/60 Hz, 120 VA Binary pump PU-4086-Binary Gradient Mixing accuracy Mixing precision 14 +/-0.4% +/-0.75% (5 ~ 95%, 0.5 ~ 20.0 mL/min) 0.2% RSD or +/-0.03min, whichever is larger. (0.5 ~ 20.0mL/min) - Dimensions, weight 300(W) x 470(D) x 150(H) mm, 21.5 kg - Power requirement AC 100 ~ 240 V, 50/60 Hz, 100 VA - Model MD-4010 MD-4015 MD-4017 Light source D2 & WI lamp D2 lamp D2 lamp Measurement range 190 ~ 900 nm 200 ~ 600 nm 200 ~ 400 nm PDA element number 1024 ch 512 ch 512 ch Slit width 1, 4, 8 nm 4 nm 4 nm Data acquisition rate Maximum 100 spectra/sec (full range) Maximum 100 spectra/sec (full range) Maximum 20 spectra/sec (full range) Noise level +/- 3.0 x 10-6 AU (specified conditions) +/- 3.0 x 10-6 AU (specified conditions) +/- 7.0 x 10-6 AU (specified conditions) Drift < 0.5 x 10-6 AU/h (specified conditions) < 0.5 x 10-6 AU/h (specified conditions) < 1.0 x 10-6 AU/h (specified conditions) Linearity 2.0 AU or more (specified conditions) 2.0 AU or more (specified conditions) 2.0 AU or more (specified conditions) Flow cell Path length: 10 mm, front loading cassette type Path length: 10 mm, front loading cassette type Path length: 10 mm, front loading cassette type PC communication USB2.0 USB2.0 USB2.0 Dimensions, weight 300(W) x 470(D) x 150(H) mm, 14.5 kg 300(W) x 470(D) x 150(H) mm, 13.5 kg 300(W) x 470(D) x 150(H) mm, 13.5 kg Power requirement AC 100 ~ 240 V, 50/60 Hz, 180 VA AC 100 ~ 240 V, 50/60 Hz, 150 VA AC 100 ~ 240 V, 50/60 Hz, 120 VA Model UV-4070 / UV-4075 Light source D2 lamp + WI lamp (UV-4070) Wavelength range 190 ~ 900 nm (UV-4070) Monochromator Czerny-T urner mount D2 lamp (UV-4075) 190 ~ 600 nm (UV-4075) Spectral bandwidth 8 nm Noise level +/-0.2 x 10-5 AU (230 nm, 1.5 sec) Drift 1 x 10-4 AU/h (230 nm, constant room temperature) Data output Maximum 100 Hz Front loading cassette cell (temperature controlled), tapered. Flow cell Path length: 10 mm Spectrum measurement (On-flow measurement) 200 ~ 900 nm (D2 & WI), 200 & 370 nm (D2), 371 ~ 900 nm (WI) (UV-4070) 200 ~ 600 nm (D2) (UV-4075), applicable measurement range; fixed T wo-wavelengths monitoring Any two wavelengths in 190 ~ 370 nm, 371 ~ 700 nm, 701 ~ 900 nm (UV-4070) Any two wavelengths in 190 ~ 370 nm, 371 ~ 600 nm (UV-4075) Dimensions, weight 300(W) x 470(D) x 150(H) mm, 10 kg Power requirement AC100 ~ 240 V, 50/60 Hz, 175VA (UV-4070), 110VA (UV-4075) Model FP-4020 (high performance model) / FP-4025 (standard model) Light source Xenon short arc lamp Wavelength setting range 200 ~ 900 nm (both EX and EM) (measurement range) 220 ~ 700 nm (both EX and EM), 200 ~ 900 nm (both EX and EM, option) Spectral bandwidth EX: 20 nm, EM: 20, 40 nm (switchable) Raman peak of water, S/N > 2300 (FP-4020)、 S/N > 1400 (FP-4025) Sensitivity (EX wavelength; 350 nm) Data output Maximum 100 Hz Flow cell Front loading cassette cell T emperature control Available setting: OFF, 4 ~ 40ºC, applicable range: [ambient -10ºC] ~ 40ºC (only for FP-4020 ) Spectrum measurement Ex and Em spectrum measurement, spectrum output, output of subtracted spectrum T wo-wavelength monitoring T wo sets of Ex/Em wavelength setting, maximum wavelength difference; 200 nm or shorter. Dimensions, weight 300(W) x 470(D) x 225(H) mm, 20 kg Power requirement AC 100 ~ 240 V, 50/60 Hz, 270VA (FP-4020), 230VA (FP-4025) Model RI-4030 RI-4035 Measurement system Deflection type Refractive index range 1.00 ~ 1.75 5.0 x 10 Linearity -5 RIU (HIGH) 5.0 x 10 5.0 x 10-4 RIU (ST D) -5 RIU (HIGH) 5.0 x 10-4 RIU (ST D) (H2O) 5.0 x 10-3 RIU (LOW) (H2O) Noise level 0.20 x 10-8 RIU or less (HIGH, ST D) 0.50 x 10-8 RIU or less (HIGH, ST D) Cell capacity 10 µL 2.7 µL Maximum usable flow- 10 mL/min (Low Flow Rate tubing) rate Maximum pressure 120 mL/min (High Flow Rate 0.1 MPa (Low Flow Rate tubing) 0.3 MPa (High Flow Rate tubing) Model CD-4095 Light source 150W Hg-Xe lamp Polarizer Glan-T aylor prism Phase modulation PEM (photo-elastic modulator) Wavelength accuracy +/- 5 nm Wavelength reproducibility +/- 0.5 nm Wavelength setting range 200 ~ 460 nm Measurement range 220 ~ 460 nm Noise level 1.2 mL/min (H2 O) Drift 0.1 MPa Data output T emperature control Ambient +10ºC ~ ambient +25ºC (setting range: 0 ~ 60ºC) Dimensions, weight 300(W) x 470(D) x 150(H) mm, 14 kg Power requirement AC 100 ~ 240 V, 50/60 Hz, 80 VA Flow cell Spectrum measurement 0.04 mdeg (at specified conditions) 0.1 mdeg/h (room temperature variation is within +/-1ºC) Maximum 100 Hz Front loading cassette cell, tapered, 44 µ L, 25 mmL 200 ~ 460 nm Dimensions, weight 300 (W) x 470 (D) x 230 (H) mm, 21 kg Power requirement AC 100 ~ 240V, 50/60 Hz, 210 VA 15 Model AS-4050/AS-4050i AS-4150 AS-4250 AS-4058 Full or partially fill loop injection (zero Full or partially fill loop injection (zero Sample injection Full or partially fill loop injection (zero sample loss available), direct line method sample loss available) injection (option, change of flow-line is required.) is required.) Number of samples 60 (2 mL vial) 180 (2 mL vial) 180 (2 mL vial) 0.1 ~ 100 µL 0.1 ~ 100 µL 0.1 ~ 100 µL Injection volume Injection precision Injection accuracy Carry over sample loss available), direct line Full or partially fill loop injection (zero injection (option, change of flow-line sample loss available) (large volume injection kit: 1 ~ 1000 µ (large volume injection kit: 1 ~ 1000 µ (large volume injection kit: 1 ~ 1000 µ 1 ~ 10000 µL L, option) L, option) L, option) RSD 0.3 % or less (specified RSD 0.25% or less (specified RSD 0.25% or less (specified conditions) conditions) conditions) +/- 0.1% or less (with correction +/- 0.1% or less (with correction +/- 0.1% or less (with correction +/- 0.1% or less (with correction function) function) function) function) 0.01% or less 0.01% or less (2 µL) (specified conditions) (2 µL) (specified conditions) 0.005% or less (with multiple solvent 0.005% or less (with multiple solvent 0.01% or less (10 µL, specified conditions) pressure Sample cooling/heating Pre-column derivatization, etc RSD 1.0 % or less (2500 µL, zero sample loss) (specified conditions) flushing valve) (specified conditions) flushing valve) (specified conditions) Maximum usable 40 (20 mL vial, standard) 0.05% or less (2000 µL, zero sample loss) (specified conditions) 0.02% or less (with multiple solvent flushing valve) (specified conditions) 30 MPa (AS-4050), 34 MPa (AS-4050i) 70 MPa 130 MPa 41 MPa (option) temp. range: 4 ~ 40ºC (option) temp. range: 4 ~ 40ºC - (option) temp. range: 4 ~ 40ºC (option) temp. range: 4 ~ 40ºC Pre-column derivatization (up to two Pre-column derivatization (up to two reagents), dilution, other user reagents), dilution, other user programs programs - Dimensions, weight 300 (W) x 470 (D) x 300 (H) mm, 21 kg 300 (W) x 470 (D) x 385.5 (H) mm, 25 kg300 (W) x 470 (D) x 385.5 (H) mm, 25 kg300 (W) x 470 (D) x 385.5 (H) mm, 25 kg Power requirement AC 100 ~ 240V, 50/60 Hz, 60 VA AC 100 ~ 240V, 50/60 Hz, 75 VA AC 100 ~ 240V, 50/60 Hz, 75 VA AC 100 ~ 240V, 50/60 Hz, 75 VA Model CO-4061 CO-4062 CO-4060 CO-4065 RO-4068 T emperature range Ambient -15ºC ~ 100ºC Ambient -15ºC ~ 100ºC Ambient -15ºC ~ 80ºC Ambient -15ºC ~ 90ºC Ambient +10ºC ~ 200ºC 280(W) x 25(D) x 60(H) mm 280(W) x 25(D) x 100(H) mm 280(W) x 25(D) x 100(H) mm 110(W) x 83(D) x 415(H) mm 270(W) x 120(D) x 415(H) mm Column compartment dimensions Heating/cooling power shut off when unusually high temperatures or solvent leaks are detected. Safety features Dimensions, weight Power consumption 300(W) x 470(D) x 150(H) mm, 300(W) x 470(D) x 150(H) mm, 150(W) x 470(D) x 465(H) mm, 300(W) x 470(D) x 465(H) mm, 150(W) x 470(D) x 150(H) mm, 10 kg 10 kg 16 kg 25 kg AC 100 ~ 240 V, 50/60 Hz, AC 100 ~ 240 V, 50/60 Hz, AC 100 ~ 240 V, 50/60 Hz, AC 100 ~ 240 V, 50/60 Hz, AC 100 ~ 240 V, 50/60 Hz, 160 VA 200 VA 350 VA 450 VA 325 VA Language English/Japanese, selectable Windows OS Windows 7 Professional 32/64 bit Windows 8.1 Professional 32/64 bit Controllable hardware LC-4000 Series, X-LC 3000 Series, LC-2000 Series, System control Direct control of instrument by Direct Mode, Maximum 4 systems control, Maximum 20 systems registration T ime programming by Control Method, Spectrum measurement, Prevention from unauthorized use by security key, Analog signal input, Up to 4 channel simultaneous input per one LC-Net II/ADC JASCO INTERNATIONAL CO., LTD. 11-10, Myojin-cho 1-chome, Hachioji, Tokyo 192-0046, Japan Tel: +81-42-649-3247 Fax: +81-42-649-3518 http://www.jascoint.co.jp/english/ Australia, China, Hong Kong, India, Indonesia, Korea, Malaysia, New Zealand, Pakistan, Philippines, Russia, Singapore, Taiwan, Thailand JASCO INCORPORATED 28600 Mary's Court, Easton, MD 21601, U.S.A Tel: +1-800-333-5272 +1-410-822-1220 Fax: +1-410-822-7526 http://www.jascoinc.com U.S.A., Canada, Costa Rica, Mexico, Puerto Rico, Argentina, Brazil, Chile, Colombia, Paraguay, Peru, Uruguay, Guatemala, , Ecuador, Bolivia JASCO EUROPE s.r.l. Via Luigi Cadorna 1, 23894 Cremella (Lc), Italy Tel: +39-039-9215811 Fax: +39-039-9215835 http://www.jasco-europe.com JASCO Deutschland www.jasco.de, JASCO UK www.jasco.co.uk, JASCO France www.jascofrance.fr, JASCO Benelux www.jasco.nl, JASCO Spain www.jasco-spain.com Italy, Germany, U.K., France, Netherlands, Belgium, Luxembourg, Spain, Sweden, Norway, Denmark, Austria, Finland, Greece, Hungary, Poland, Portugal, Romania, Switzerland, Algeria, Cyprus, Egypt, Israel, Jordan, Kuwait, Lebanon, Morocco, Saudi Arabia, South Africa, Tunisia, Turkey, U.A.E., Yemen 12 kg
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