LC 4000 0926 Print0

User Manual: LC-4000

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LC-4000 Series

Integrated HPLC and UHPLC systems

For over two decades JASCO

analytical HPLC pumps have

employed an asymmetric twin-

piston solvent delivery system

SSQD (Slow Suction, Quick Delivery)

which provides significantly better

flow and pressure profiles than the

commonly used conventional twin-

piston reciprocating design. In the

LC-4000 series, SSQD has been

re-developed, with a completely

new solvent delivery mechanism

ExReFT (Extremely Reliable Flow

Technology) offering the highest

stability in solvent delivery across

the entire analytical flow rate range

used in the PU-4180/85 RHPLC and

PU-4280/85 UHPLC pump models.

A quick brief - LC-4000 Series

The LC-4000 Series is the latest in a long history of innovative

HPLC systems developed by JASCO reaching all the way back to

the start of commercial HPLC in the early 1970s.

The concept of the integrated LC-4000 series HPLC provides

key separation platforms at 50MPa, 70MPa and 130MPa which

correspond to conventional HPLC, the increasingly popular

Rapid Analysis (RHPLC) and sub 2um UHPLC, respectively.

Each platform is supplied with a dedicated pump and

autosampler matched to the operating pressure and all three

platforms share common detectors optimized for high-speed

100Hz acquisition and the narrow peak shapes common to

both RHPLC and UHPLC.

Advances in Solvent Delivery

Pioneering Optical Design

As a pioneer in optical spectroscopy, JASCO has access

to optical designs that few can match. Adopting designs

from some of our more powerful spectrometers we

have developed a range of HPLC optical detectors with

unrivalled performance, like the new class leading FP-

4020 fluorescence detector with S/N of over 2300:1, the

latest high-speed refractive index detector with micro cell

optimized for RHPLC and UHPLC and a completely revised

circular dichroismdetector for chiral chromatography.

New Technologies for Method Development

With the range of column technologies becoming ever more

diverse - the traditional 5um fully porous particle is finally

losing ground to some of the newer technologies such as the

new superficially porous solid core (Coreshell) and the wide

variety of sub-2um particles, the requirements for method

development are becoming more demanding. The LC-4000

series has a number of newly developed accessories to help

you make better separations faster, with accessories such

as, flexible and stackable column ovens in a range of sizes to

accommodate analytical to preparative columns and multiple

columns at every scale and with options for integrated 6 or

10 solvent and column selection valves.

The LC-4000 Series pump modules requires smaller bench

space than ordinary HPLC pumps. An integrated solvent

delivery system can be incorporated into a single pump

module.

ChromNAV, the cross-platform

chromatography data system, is evolving

too. Version 2.0 now offers many new

features and a great new look to make

working with the LC-4000 HPLC system

an even better user experience.

Each LC-4000 module

is designed for easy

maintenance.

Front-panel access to light

source and consumables

Compact and Easy to Use

Despite the extra power delivered by the LC-4000 series

HPLC, the standard footprint is only 300 mm wide, a standard

system requires only a very small amount of bench space.

For easy user-maintenance, all LC-4000 instruments feature

front-access for replacing consumables such as pump

components and light sources, even the autosampler

consumables such as the syringe parts are easy to access.

For those users who require front-panel control, the LC-

4000 series retains the popular keypad and display (with a

backlit LED display as standard). Keypad control can still be

configured for convenient override when under PC control.

Front panel control is included as standard

System Conguraons

UHPLC Analycal Systems at 130MPa

Ten years after the new segment of the HPLC market, UHPLC has found a niche for

those that need to run a large number of samplers or get results quickly. Pioneering

column technology is pushing the particle size even smaller. The near 20,000 psi

operation of the LC-4000 130MPa is designed to take advantage of the separation

efficiency of the very small particles couple with longer and narrower columns. The

LC-4000 130MPa system incorporates many of the same features as the 70MPa

system, but with materials designed to withstand higher pressures.

Semi-prep HPLC LC-4000 system

Convenonal HPLC and RHPLC Analycal Systems up to 70MPa

The LC-4000 Series 70MPa system has been designed to future-proof your HPLC

requirements. The LC-4000 70MPa system can be used with conventional 3 and 5µm

particle size analytical columns at typical lower pressures around 10 to 20MPa (1500

to 3000psi) and can also be used with smaller particles such as shorter length UHPLC

columns and superficially porous (SPP) or Coreshell that require slightly higher

pressure solvent delivery to provide optimum linear velocity through the column.

Preparave HPLC Systems

The LC-4000 preparative HPLC systems includes three platform options

Up to 20mL/min for columns up to 21.2mm ID

Up to 50mL/min for columns up to 3cm ID

Up to 120mL/min for columns up to 5cm ID

Large column oven for multiple preparative columns and optional automatic column

switching

Preparative detector options including high flow short path length flow-cells

Open-bed fraction collector with sample trays for tubes, plates or large containers

ChromNAV-FC for fraction control using time, threshold or slope from up to 4 different

signals

The LC-4000 series HPLC analytical and preparative HPLC systems

have a number of common features

300mm wide footprint

Each system component is completely stackable into a modest height

The system is fully integrated with complete system monitoring

All user replaceable components are accessible through the front panel

Vertical leak/drain system to channel away solvent

Integrated earthquake securing option

All detectors include 100Hz acquistion with interchangeable flowcells

LC-4000 Series Extensive Range of Modules

The LC-4000 Series, enables multiple system design based on the following modules. This concept can be used

to create many different system configurations to match your application.

The extensive range of detectors based on JASCO's spectroscopy technology is a key factor in providing a

solution to virtually any application.

DETECTORS

PUMPS

AUTOSAMPLERS COLUMN OVENS

HPLC

AS-4050/50i

CO-4065 Prep

RO-4068

Reaction oven

RHPLC

AS-4150

UHPLC

AS-4250

Preparative

AS-4058

C O - 4 0 6 1 / 6 2

compact

CO - 40 60 high

capacity

PREP

PU-4086/87

PU-4086B

Fluorescence

FP-4020/25

Refractive Index

RI-4030/35

PDA

MD-4010/15/17

HPLC, RHPLC

PU-4185/80

PU-4185B

UHPLC

PU-4285

PU-4285B

Optical rotation

OR-4090

UV/Vis visible

UV-4070/75

Circular Dichroism

CD-4095

The LC-4000 series HPLC pumps include expansion bays that allow a simple pump module to be

transformed into a powerful solvent delivery system. Accessory modules can be installed in the LC-4000

series pump for use with virtually any solvent delivery requirement.

The LC-4000 Series next generation HPLC pumps with flexible expandability

Mobile phase: Acetonitrile/Water (80/20)

Flow Rate: 1.0mL/min

Column: CrestPak C18S (4.6mmI.D. x 150mm, 5µm)

Column temerature: 40 deg C

Detection wave length: 260nm, Response; 1sec

Sample: Polycyclic aromatic hydrocarbon (10µg/mL each)

(Naphthalene, Fluorene, Anthracene, Pyrene, Chrysene, Benzo[a]pyrene)

Injection: 10µL

Excellent ow rate repeatability

PU-4180 Flow rate accuracy

(1.0mL/min, n=10)

Retention time reproducibility (n=10)

PU-4180

Analytical pump platform

PU-4185

Semi micro pump platform

Average

Standard diviation（n-1）

CV%（n-1）

Naphthalene

3.1400

3.1417

3.1400

3.1416

3.1400

3.1405

0.001

0.025

Fluorene

3.9367

3.9683

3.9367

3.9383

3.9367

3.9370

0.001

0.018

Anthracene

4.5000

4.5017

4.5000

4.5002

4.5000

4.5016

4.5000

4.5017

4.5005

0.001

0.017

Pyrene

5.6283

5.6317

5.6300

5.6283

5.6300

5.6317

5.6300

5.6301

5.6299

0.001

0.022

Chrysene

6.4934

6.4950

6.4933

6.4950

6.4933

6.4967

6.4950

6.4949

6.4950

6.4947

0.001

0.016

Benzo[a]pyrene

9.4267

9.4300

9.4285

9.4283

9.4317

9.4297

0.002

0.020

The problems caused by pressure

variations in solvent delivery when

switching between the two pump

heads has been improved with a

new design that precisely controls

the plunger drive. The LC-4000

series analytical pumps offer flow

rate precision with RSDs of better

than 0.05% (difficult to achieve with

commercially available pumps). The

PU-4180 and 4185 pumps operate

at pressures up to 70 MPa, designed

mainly for use with RHPLC for core-

shell columns or similar micro

packing materials as well as working

at pressures commonly used for

conventional HPLC.

The PU-4280 and PU-4285 UHPLC

pumps provide precise flow at

pressures up to 130 MPa; for columns

with particle sizes of less than 2 µm.

The LC-4000 series UHPLC system can

also be used for rapid analysis HPLC

(RHPLC) and conventional HPLC.

Upgrade and Expansion

The unique expansion bays in the pump platform can be fitted with plug-in

modules to quickly and easily transform a simple isocratic pumping system

into a sophisticated multi-solvent delivery system by installing a second pump,

degasser, quaternary gradient unit, solvent selection valve or dynamic mixer

without increasing the footprint.

Adding a second pump module enables an isocratic pump to be upgraded to a

dual pump system for either binary gradient or two isocratic flows.

A powerful configuration of the single-unit design is the quaternary gradient

solvent delivery system including degasser, with a post-column reagent pump

configured in a compact chassis only 300mm wide.

Eliminang pulsaon

The LC-4000 Preparative-HPLC

System includes pumps at three

scales. The PU-4086 pump offers

flow rates up to 20 mL/min, the

PU-4087 pump up to 50 mL/min

and the PU-4088 up to 120mL/

min. Both single and multi-pump

gradient modes can be used with

the PU-4086 pump platform whilst

the PU-4087 and PU-4088 are

used in multi-pump mode only.

The new MK2 technology used in

the PU-4086 and PU-4087 motor

driver control has been increased

so that the maximum pressure

can be used at higher flow-rates

for all pump models. Solvents can

be delivered at higher flow rates

and higher pressures for use with

longer preparative HPLC columns

that generate more back pressure

and for faster and more productive

preparative separations.

Dynamic mixers, solvent selection

valves and the prep degasser can

be installed in the expansion bays

in both the PU-4086 and PU-4087

modules.

Mobile phase: A; Water, B; Acetonitrile

Gradient: (A/B) (65/35) → (5/95) Linear Gradient

Flow rate: 1.0mL/min

Column: CrestPak C18S (4.6mmI.D. x 150mm, 5µm)

Column temerature: 40 deg C

Detection wave length: 254nm, Response; 1sec

Sample: Alkylphenon samles 10µg/mL (Benzohenon 5µg/mL)

(Acetanilide, Acetophenone, Propiophenone,

Butyrophenone, Benzophenone, Valerophenone, Hexanophenone,

Heptanophenone,Octanophenone)

Injection: 10µL

Retention time reproducibility (n=10)

PU-4185-Binary pump

PU-4086 semi prep pump

Recycling inlet switching unit, Recycling valve

unit (Option)

Two pump binary gradient

PU-4180 Quaternary gradient mixing performance

(1.0mL/min, n=10)

PU-4180

(compact quaternary gradient pump with

micro-volume degasser ) *Option

Average

Standard diviation (n-1)

CV% (n-1)

Acetanilide

3.117

3.113

3.117

3.116

0.001

0.034

Acetophenone

5.617

5.620

5.617

5.620

5.618

0.002

0.029

Propiophenone

7.590

7.593

7.590

7.593

7.591

0.002

0.021

Butyrophenone

9.303

9.307

9.310

9.307

9.310

9.313

9.308

0.003

0.030

Benzophenone

9.950

9.953

9.957

9.953

0.002

0.025

Hexanophemone

12.583

12.587

12.590

12.593

12.588

0.003

0.026

Heptanophenone

14.067

14.073

14.070

0.002

0.016

Octanophenone

15.430

15.433

15.437

15.434

0.002

0.012

Valerophenone

10.980

10.983

10.987

10.983

10.987

10.990

10.985

0.003

0.026

Pump Conguraons

The PU-4180/85 and PU-4280/85 analytical pumps can

be configured for both single pump gradient and multiple

pump gradient operation. The single pump quaternary

gradient module offers excellent gradient formation for

up to four solvents. The newly designed 4-way solvent

valve for switching the four solvents is synchronized

to the operation of the pump motor control - variable

cycle adaption optimizes delivery according to gradient

resolution and flow rate. This highly efficient quaternary

gradient mode can be applied to a wide flow-rate range

from low to high flow rates.

Two and three pump binary or ternary gradient modules

with optional built-in degasser are available for fast cycle

high-resolution gradient formation; the response time is

superior to the single pump quaternary gradient system.

Solvent Mixing

Multi-pump gradient mixing is done using a variable speed

dynamic mixer with a range of a small volume mixing

chambers incorporating a micro-stirrer to provide the

smallest possible dead volume, offering higher mixing

efficiencies for all HPLC solvents.

The optional in-line TERA Mixer is a turbulent-flow solvent

mixer uniquely designed to offer high efficiency mixing

with minimal dead volume.

Solvent Degassing

The in-line vacuum degasser module uses a new Teflon

AF membrane with a hold-up volume of only 400µL (per

solvent line); this offers very quick solvent replacement

with minimal waste. Despite its small size, the new

degasser offers more effective and stable degassing

than larger previous generation models. A degasser for

preparative-HPLC is also available.

Preparave and Semi-preparave Pumps

Threshold 50000

Threshold 10000

0.0

100000

200000

220000

0.2 0.4 0.6 0.8 10.0 12.0 14.0

Retention Time[min]

Intensity[µV]

UV spectra auto-scan

Automatic spectral scannning can be

controlled using ChromNAV, when a peak

exceeds a set threshold a scan is executed

automatically.

MD-4010, MD-4015

and MD-4017 PDA Detectors

The LC-4000 Series includes three

PDA detectors. The MD-4017 is

a useful replacement for a single

wavelength detector, the MD-4015

is the workhorse for UV-visible

detection in the range 200 to 600nm

and the MD-4010 is designed for high

sensitivity and high spectral and time

resolution.

The MD-4010 PDA detector offers

the highest detection sensitivity,

equivalent to a single wavelength

detector. Making use of a 1024

element photometric diode array,

the measurement wavelength range

is from 190nm to 900nm with a

resolution of 1nm and fast spectrum

acquisition rate of up to 100 spectra/

sec. When used with the new

temperature controlled SP type

semi-micro flow cell the MD-4010

is well suited to the narrower peaks

typically found with UHPLC or RHPLC

separations. The MD-4010 is fitted

with an internal mercury lamp for

automatic wavelength calibration.

The MD-4015 PDA detector is the

routine detector for conventional

HPLC, RHPLC and UHPLC. The

sensitivity is almost equivalent to

that of the MD-4010 but only covers

the wavelength range from 200nm to

600nm using a 512 element PDA for

1nm resolution and with high speed

100Hz spectrum acquisition.

With JASCO’s long history of spectrometer development, you will nd that each detector is well

designed; these next generation detectors employ the latest optical and electronic technologies for high

sensitivity, excellent stability and easy maintenance.

A comprehensive range of high performance opcal detectors

UV-4070 UV Visible

The MD-4017 detector has a price-point comparable

to a typical single wavelength detector and has been

designed for the user only interested in the UV region

from 200-400nm, the resolution is excellent at 1nm and

the maximum spectrum acquisition is 20 spectra per

second (20Hz), useful for peak widths as small as 1 to 2

seconds.

When used with ChromNAV 2.0 CDS the PDA detector

offers multi-functional analysis with a variety of

functions such as spectral analysis, library search for

peak identification, peak purity and multi-wavelength

quantitation.

UV-4070/4075UV-Visible

Detectors

Using the advanced Czerny-

Turner monochromator design

with optimization of the optical

system, baseline change due to

refractive index is minimized.

Baseline fluctuation from changes

in environmental temperature

is reduced with a temperature-

controlled cell, baseline drift caused

by temperature fluctuation in the

light source is also reduced with a

temperature controlled light source.

The result is excellent stability with

the noise level +/- 0.2x10-5 AU and

drift 1x10-4 AU/h. Both detectors offer

simultaneous dual-wavelength data

acquisition and spectral scanning.

User maintenance can be done

without removing the module from

the system. Front-panel access is

used for light source replacement and

automatic wavelength calibration can

be carried out using a mercury lamp.

Both detectors include high speed

data acquisition at up to 100Hz and

can be used for conventional HPLC

with the standard flow cell and for

UHPLC/RHPLC when used with the SP

type semi-micro cell.

Spectral measurement view

200

-0.01

0.1

0.2

0.24

400 600 800 900

Wavelength[nm]

Abs

UV detector Auto scan parameters

ChromNAV PDA data processing

included as standard

Front panel access for easy lamp

replacement

MD-4010 PDA

Refracve Index detector RI-4030/4035

The Refractive Index detectors include the optical system mounted in a precisely

temperature-regulated housing to eliminate the influence of changes in ambient

temperature. The RI-4030 and 4035 detectors are fully stable just one hour

after powering on. The RI-4030 can be used for both analytical and preparative

scales. In preparative mode the maximum flow rate is 120 ml/min. The RI-4035

is a dedicated semi-micro RI detector with a micro-volume flow cell designed for

use with semi-micro GPC analysis using GPC columns with a fine pore size. The

100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC

separations. The LED light source in both detectors has a long life time (over

10,000 hours) for low maintenance operation. Both the RI-4030 and RI-4035

detectors can be fitted with an optional pressure release safety-valve to prevent

damage to the flow cell in the event blockage.

Circular Dichroism detector CD-4095

The CD-4095 is a unique HPLC detector for measuring circular dichroism

developed from the market leading J-Series circular dichroism

spectropolarimeter. Optically active compounds with a chromophore close to a

chiral center may absorb circularly polarized light, which can be detected with

excellent sensitivity and selectivity. When chiral compounds are measured using

a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even

when separated by a chiral column. The CD-4095 circular dichroism detector

makes it possible to positively distinguish between the d- and l- enantiomers

by measuring the positive and negative peaks. It is also possible to quantify the

d- and l-forms even when they are not separated, a calibration curve based on

the g-factor can be used to determine the enantiomeric ratio. The ChromNAV

CDS can be used to simultaneously measure the CD, g-factor data and UV-visible

signal. The CD spectra of eluted compounds can be measured by triggering

scanning manually, by timed program or automatically by threshold.

Chiral detector OR-4090

The OR-4090 Chiral detector measures the angle of rotation of plane polarized

light caused by optical active isomers and is useful for chiral compounds with no

absorption. The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing

a strong output in the UV/Visible region where the largest optical rotations offer

the highest sensitivity. The wide wavelength range from 350-900nm also allows

measurement of a wide variety of samples. One of the key benefits of optical

rotation detection is that it is unaffected by changes in solvent and can be used

with gradient elution, unlike an RI detector.

0.0 1.0 2.0 3.0 4.0 5.0

Retention Time[min]

Intensity[µV]

Fluorescence detector

FP-4020/4025

The FP-4020 fluorescence detector has been designed for the pursuit of extreme

sensitivity. Re-designing the optics and electronics from the ground up, as well

as removing the effects of ambient temperature with a new temperature-

regulated cell body has led to the highest sensitivity with stable detection. The

new axially irradiated cell design enables the very highest sensitivity detection.

These changes have resulted in a giant leap in sensitivity for the FP-4020 to

achieve a signal noise ratio of 2300:1 or higher (based on the water Raman

peak). The FP-4025 is an excellent routine fluorescence detector that has a

slightly lower specification with a water Raman S/N of 1400:1 .

Both detectors can be used for simultaneous detection at two wavelength pairs;

excitation and emission wavelengths can be changed during the acquisition using

a time program and automatic spectra scanning can be triggered manually, by

time or automatically by threshold. A built-in mercury lamp enables automatic

wavelength correction and the front-access design allows easy replacement of

the flow cell assembly and light source.

Anthracene 1.4 fg (S/N=3)

(Anthracene 100 fg Injection)

High sensitivity measurement of Anthracene Dual wavelength simultaneous measurement

26000

20000

10000

FP-4020 Fluorescence

RI-4030 Refractive Index

CD-4095 Circular Dichroism

OR-4090 Optical Rotation

Autosamplers - AS-4050/4050i/4150/4250/4058

The LC-4000 Series autosamplers provide a range of options to suit any sample

injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC

model or the AS-4058 preparative scale autosampler.

The AS-4050 is a 60 sample autosampler for use with conventional HPLC with options

for analytical and semi-preparative injection volumes. The AS-4150 and AS-4250 are

high-capacity, high performance autosamplers designed for RHPLC (up to 70MPa)and

UHPLC (up to 130MPa), respectively, with a minimum injection cycle-time of only 30

seconds. Peltier cooling is available for all autosamplers and light protection for photo-

labile samples is available on the higher level models.

Pre-injection sample preparation is available on the high performance autosamplers

with programmable options for sample dilution and pre-column derivitization.

The injection mode can be switched between full loop injection and partial loop

injections.

Up to 5 different washing solvents can be used to achieve excellent carry-over at less

than 0.005%.

An optional degasser can be installed for degassing up to 5 flushing solvents.

The AS-4058 is the LC-4000 Series largest volume autosampler with injection volumes

up to 5mL as standard (up to 10mL as an option). The AS-4058 is typically used in

combination with either a PU-4087 or PU-4088 pump for preparative separations.

All LC-4000 series autosamplers share the same simple maintenance features, with

consumable parts such as injection ports, rotor seals, and loops accessible from the

front panel for easy replacement.

AS-4050 60 sample HPLC

autosampler

AS-4250 High capacity UHPLC

autosampler

Designed for easy maintenance

AS-4050/4150/4250/4058 flow diagram (loop injection)

Metering Tube

Solvent-stop

Valve

Flushing

Port

3way

Valve

Metering

Pump

Flushing

Solvent

Air Pump

Air-drain

Valve Waste

Bottle Needle

Injection

Port

Sample

Vial

Sample

loop

Injection

Valve

Column

To Detector

HPLC Pump

Simple replacement

of syringe in AS-4050

UniFinePak HPLC Columns

UniFinePak is a new type of column that uses a hybrid packing material. Compared

with traditional packing materials that use silica as the base material, UniFinePak

columns can be used with a wider pH range from alkali to acid. UniFinePak columns

offer an extended analysis range due to its chemical strength and resilience to high

pressure operation.

The entire range of UniFinePak columns uses a common packing material in a range

of particle sizes from semi-micro to analytical to semi preparative scales, which

simplifies method development when scaling-up separations from analytical to semi-

preparative .

Oponal Preparave HPLC fracon collecon kit

The LC-4000 series preparative system fraction collection kit includes

Fraction collector

Flow divertor valve

ChromNav FC fraction control program for ChromNAV

CO-4060

CO-4061

Method Conversion Program

Column Ovens

The LC-4000 Series column ovens have

been designed to provide excellent

utility for a wide range of different user

requirements. For simple requirements

the stackable CO-4061 can be used with

column lengths up to 200mm (or up to

300mm with an optional extension).

For RHPLC and UHPLC the CO-4062 has

capacity for up to six 150 mm length

columns, with optional one or two

internally mounted automatic switching

valves for 2 or 6 column selection.

For GPC and larger preparative columns,

select either of the two high-capacity CO-

4060 or CO-4065 ovens. The CO-4060

can accommodate columns with lengths

up to 300mm or larger ID preparative

columns. The CO-4065 can accommodate

up to 10 columns with lengths up to

300mm and a pair of column selection

valves is included as standard.

All column ovens have built-in

electronic cooling for controlling

column temperature at sub-ambient

temperatures.

The CO-4062 and CO-4061 block-heater

type ovens can be fitted with an optional

micro-volume pre-heating unit for pre-

heating the mobile phase before the

column to improve resolution and peak

shape.

The RO-4068 reaction oven is used for

high temperature post-column sample

processing with capacity for columns and

reaction coils.

Analysis conditions

Comparison of Toluene peaks

WIth Pre coulmn stabilizer —

WIthout Pre coulmn stabilizer —

WIth Pre coulmn stabilizer —

WIthout Pre coulmn stabilizer —

Toluene

Temp : 25 degree C

Temp : 40 degree C

Efciency comparison of stabilizer

600000

400000

200000

600000

400000

200000

0.0 0.4 0.8 1.2 1.6 2.0 2.4 2.8

IntensityIntensity

Column X-Presspak VC18-W

(3 mmI.D. x 50 mmL, 2 µm)

Eluent Water / Acetonitrile (Gradient)

Flow Rate 1.0 mL/min

Sample Gasoline Oxidization Products

Room Temp. Oven Temp. Pre-Column

Stabilizer

Theoretical Peak Width tR(min)

21˚C

25˚C With 56095 0.019 1.893

Without 54747 0.019 1.887

40˚C With 46021 0.020 1.799

Without 36402 0.023 1.851

ChromNAV 2.0 The 'PowerTool' for HPLC Analysis

ChromNAV 2.0 (and ChromNAV-CFR 2.0) are JASCO’s next generation CDS developed from the powerful

and easy-to-use ChromNAV 1.0 with a host of exciting new features.

With a customizable graphical-user-interface (GUI), the user can set-up the system to display only the

functions necessary for their application. This latest intuitive GUI allows the user to quickly learn the

operation and explore the extensive functionality for data processing.

ChromNAV 2.0 is a universal CDS which can be used with any type of separation - HPLC, UHPLC, RHPLC,

prep-HPLC, analytical SFC and prep-SFC. ChromNav can also satisfiy the demands of dedicated analysis

and multi-purpose systems.

PDA analysis

PDA data processing is included as standard. Data is displayed

in 2D contour plot and 3D with simultaneous overlay of

spectra and chromatograms.Extract chromatograms at single

or multiple wavelengths for quantitation

ChromNAV 2.0 offers powerful system

control and data acquisition with

options for 2D (or up to 4D) gradient

control and multi-zone oven control

with control of up to 3 column ovens

for method development. Solvent and

column selection control is increased

to include up to 10 positions for both.

During acquisition, the run-time for

a chromatogram can be increased to

capture later eluting peaks which may

exceed the set run-time.

Previously acquired chromatograms

can be overlaid for visual comparison

with the data currently being

acquired, as a quick indicator to

the user of current separation

performance.

Data acquisition and analysis for a

PDA detector are included as standard

in ChromNAV.

The fluorescence and UV-visible

detectors include spectral scanning

as standard, which can be triggered

manually, by time or by threshold.

A newly added feature of ChromNAV

2.0 is the automatic message that can

be emailed to your PC or smartphone

to give an update on the progress of

the sequence.

With the increasing requirements

for Green Energy, the LC-4000 series

has the facility not only to power off

and save lamp life at the end of the

run, but also a ‘soft-off’ function to

automatically power off a module at

the end of the sequence.

Control method

The pump flow rate and gradient profile display is

flexible and can be overlaid with a chromatogram

for adjusting gradient conditions.

ChromNAV 2.0

Oponal Applicaons

• ChromNAV CFR for 21 CFR Part 11 compliance and electronic registration of data

• GPC molecular weight dispersion

• Molecular weight dispersion of low molecular weight heparin

• FUMI theory (Function of Mutual Information) for theoretical precision analysis

ChromNAV-GPC

Molecular weight distribution program ( Option )

ChromNAV GPC/SEC calculation program for GPC analysis

The optional ChromNAV-FC fraction

collector control is available as

part of the Prep-HPLC kit and

provides ChromNav with the

capability to control the LC-4000

Series preparative HPLC system for

fraction collection.

ChromNAV-FC offers control for

two different capacity fraction

collectors, with sophisticated

fractionation control with several

methods for peak collection, such

as time, threshold and slope, and

can even be programmed to use

data collected simultaneously

from multiple detectors such

as UV-visible, refractive index

or circular dichroism. Fraction

simulation is applied to an acquired

chromatogram to optimize the

fraction collection parameters and

the graphical display can be used

to locate peaks of interest in the

fraction collector tray.

ChromNAV-FC also supports

additional functions for prep-HPLC

applications, including automatic

multi-channel fractions, fraction

repeat and fraction re-cycle.

*1: ChromNAV CFR is an optional

software package for ChromNAV.

ChromNav 2.0 standard features

• Peak integration and peak identification

• Peak grouping

• Linear and non-linear quantitation

• 3D chromatogram analysis,

• Spectral analysis for UV-visible, Fluorescence and PDA detectors

• Style report generator

• User calculation

All raw data is protected and saved; which can then be analyzed and re-analyzed, reported and saved with both the raw

data and with any data processing from the user’s analysis.

A comprehensive audit trail records the acquisition method along with a history of instrument performance indicators in

each sample data file, which provides the user a snap-shot of the condition of the system during the run and can warn about

requirements for impending maintenance.

ChromNAV- FC Collection view window ( Option)

ChromNAV-FC fraction collector control for the CHF-

122SC fraction collector

ChromNAV-FC for preparave HPLC

Pump

Model PU-4185 PU-4180 PU-4285

Flow rate setting range 0.001 ~ 4.0 mL/min 0.001 ~ 10.0 mL/min 0.001 ~ 2.0 mL/min

Applicable flow rate

range

0.05 ~ 3.0 mL/min (~ 70 MPa)

~ 4.0 mL/min (~ 40 MPa)

0.5 ~ 6.0 mL/min (~ 70 MPa)

~ 10.0 mL/min (~ 35 MPa)

0.050 ~ 1.5 mL/min (~ 130 MPa)

1.501 ~ 2.0mL/min (~ 100 MPa)

Maximum pressure 70 MPa (~ 3.0 mL/min)

40 MPa (~ 4.0 mL/min)

70 MPa ( ~ 6.0 mL/min)

35 MPa (~ 10.0 mL/min)

130.0 MPa (~ 1.5 mL/min)

100.0 MPa (~ 2.0 mL/min)

Flow rate accuracy +/- 1% or +/- 2 µL/min, whichever is larger.

(0.05 ~ 4.0 mL/min)

+/- 1% or +/- 2 µL/min, whichever is larger.

(0.5 ~ 10.0 mL/min)

+/- 1% or +/- 2 µL/min, whichever is larger.

(0.05 ~ 2.0 mL/min)

Flow rate precision

0.05% RSD or +/- 0.04min SD, whichever is larger.

(0.05 ~ 4.0 mL/min)

(measurement by chromatogram)

0.05% RSD or +/- 0.04min SD, whichever is larger.

(0.5 ~ 5.0 mL/min)

(measurement by chromatogram)

0.05 % RSD or +/- 0.04 min SD, whichever is

larger. (0.1 ~ 2.0 mL/min)

(measurement by chromatogram)

Dimensions, weight 300(W) x 470(D) x 150(H) mm, 13 kg 300(W) x 470(D) x 150(H) mm, 13 kg 300(W) x 470(D) x 150(H) mm, 14.5 kg

Power requirement AC 100 ~ 240 V, 50/60 Hz, 80 VA AC 100 ~ 240V, 50/60 Hz, 80 VA AC 100 ~ 240V, 50/60 Hz, 60 VA

(option) temp. range: 4 ~ 40ºC

Gradient mode with low-pressure gradient unit

Mixing accuracy +/-0.6% (5 ~ 95%, 0.2 ~ 1.0 mL/min)

+/-1.2% (5 ~ 95%, ~ 4.0 mL/min)

+/-0.8% (5 ~ 95%, 0.5 ~ 5.0 mL/min) +/-0.6% (5 ~ 95%, 0.2 ~ 1.0 mL/min)

+/-1.2% (5 ~ 95%, ~ 2.0 mL/min)

Mixing precision

0.15% RSD or +/- 0.01min, whichever is larger.

(0.2 ~ 4.0 mL/min)

(measurement by chromatogram)

0.25% RSD or +/-0.02min, whichever is larger.

(0.5 ~ 5.0 mL/min)

(measurement by chromatogram)

0.15% RSD or +/-0.01min, whichever is larger.

(0.2 ~ 2.0 mL/min)

(measurement by chromatogram)

Binary pump PU-4185-Binary PU-4285-Binary

Gradient mode

Mixing accuracy +/-0.4%

(5 ~ 95%, 0.2 ~ 4.0 mL/min)

-+/-0.4%

(5 ~ 95%, 0.2 ~ 2.0 mL/min)

Mixing precision

0.15% RSD or +/-0.01 min, whichever is larger.

(0.2 ~ 4.0 mL/min)

(measurement by chromatogram)

0.15% RSD or +/-0.01 min, whichever is larger.

(0.2 ~ 2.0 mL/min)

(measurement by chromatogram)

Dimensions, weight 300(W) x 470(D) x 150(H) mm, 19.5 kg - 300(W) x 470(D) x 150(H) mm, 22.5 kg

Power requirement AC 100 ~ 240 V, 50/60 Hz, 130 VA - AC 100 ~ 240 V, 50/60 Hz, 110 VA

Model PU-4086 PU-4087

Flow rate setting range 0.001 ~ 20.0 mL/min 0.01 ~ 50.0 mL/min

Applicable flow rate

range

0.5 ~ 20.0 mL/min

(0 ~ 50 MPa)

5.0 ~ 30.0 mL/min (~ 50 MPa)

30.01 ~ 40.0 mL/min (~ 40 MPa)

40.01 ~ 50.0 mL/min (~ 30 MPa)

Maximum pressure 50 MPa

50 MPa (~ 30.0 mL/min)

40 MPa (~ 40.0 mL/min)

30 MPa (~ 50.0 mL/min)

Flow rate accuracy +/- 1% or +/- 10 µL/min, whichever is larger.

(0.5 ~ 20.0 mL/min)

+/- 1% or +/- 50 µL/min, whichever is larger.

(5.0 ~ 50.0 mL/min)

Flow rate precision

0.05% RSD or +/- 0.04min SD, whichever is larger.

(0.5 ~ 20.0 mL/min)

(measurement by chromatogram)

0.1% RSD or +/- 0.04min SD, whichever is larger.

(5 ~ 40 mL/min)

(measurement by chromatogram)

Dimensions, weight 300(W) x 470(D) x 150(H) mm, 14.5 kg 300(W) x 470(D) x 150(H) mm, 18 kg

Power requirement AC 100 ~ 240 V, 50/60 Hz, 90 VA AC 100 ~ 240 V, 50/60 Hz, 120 VA

Binary pump PU-4086-Binary

Gradient

Mixing accuracy

+/-0.75% (5 ~ 95%, 0.5 ~ 20.0 mL/min) -

Mixing precision 0.2% RSD or +/-0.03min, whichever is larger.

(0.5 ~ 20.0mL/min)

Dimensions, weight 300(W) x 470(D) x 150(H) mm, 21.5 kg -

Power requirement AC 100 ~ 240 V, 50/60 Hz, 100 VA -

Specicaons

Model UV-4070 / UV-4075

Light source D2 lamp + WI lamp (UV-4070)

D2 lamp (UV-4075)

Wavelength range 190 ~ 900 nm (UV-4070)

190 ~ 600 nm (UV-4075)

Monochromator Czerny-Turner mount

Spectral bandwidth 8 nm

Noise level

+/-0.2 x 10

-5

AU (230 nm, 1.5 sec)

Drift

1 x 10

-4

AU/h (230 nm, constant room temperature)

Data output Maximum 100 Hz

Flow cell Front loading cassette cell (temperature controlled), tapered.

Path length: 10 mm

Spectrum measurement 200 ~ 900 nm (D2 & WI), 200 & 370 nm (D2), 371 ~ 900 nm (WI) (UV-4070)

(On-flow measurement) 200 ~ 600 nm (D2) (UV-4075), applicable measurement range; fixed

Two-wavelengths monitoring Any two wavelengths in 190 ~ 370 nm, 371 ~ 700 nm, 701 ~ 900 nm (UV-4070)

Any two wavelengths in 190 ~ 370 nm, 371 ~ 600 nm (UV-4075)

Dimensions, weight 300(W) x 470(D) x 150(H) mm, 10 kg

Power requirement AC100 ~ 240 V, 50/60 Hz, 175VA (UV-4070), 110VA (UV-4075)

Model FP-4020 (high performance model) / FP-4025 (standard model)

Light source Xenon short arc lamp

Wavelength setting range 200 ~ 900 nm (both EX and EM)

(measurement range) 220 ~ 700 nm (both EX and EM), 200 ~ 900 nm (both EX and EM, option)

Spectral bandwidth EX: 20 nm, EM: 20, 40 nm (switchable)

Sensitivity Raman peak of water, S/N > 2300 (FP-4020)、S/N > 1400 (FP-4025)

(EX wavelength; 350 nm)

Data output Maximum 100 Hz

Flow cell Front loading cassette cell

Temperature control

Available setting: OFF, 4 ~ 40ºC, applicable range: [ambient -10ºC] ~ 40ºC (only for FP-4020 )

Spectrum measurement Ex and Em spectrum measurement, spectrum output, output of subtracted spectrum

Two-wavelength monitoring Two sets of Ex/Em wavelength setting, maximum wavelength difference; 200 nm or shorter.

Dimensions, weight 300(W) x 470(D) x 225(H) mm, 20 kg

Power requirement AC 100 ~ 240 V, 50/60 Hz, 270VA (FP-4020), 230VA (FP-4025)

Model MD-4010 MD-4015 MD-4017

Light source D2 & WI lamp D2 lamp D2 lamp

Measurement range 190 ~ 900 nm 200 ~ 600 nm 200 ~ 400 nm

PDA element number 1024 ch 512 ch 512 ch

Slit width 1, 4, 8 nm 4 nm 4 nm

Data acquisition rate Maximum 100 spectra/sec (full range) Maximum 100 spectra/sec (full range) Maximum 20 spectra/sec (full range)

Noise level

+/- 3.0 x 10

-6

AU (specified conditions) +/- 3.0 x 10

-6

AU (specified conditions) +/- 7.0 x 10

-6

AU (specified conditions)

Drift

< 0.5 x 10

-6

AU/h (specified conditions) < 0.5 x 10

-6

AU/h

(specified conditions) < 1.0 x 10

-6

AU/h (specified conditions)

Linearity 2.0 AU or more (specified conditions) 2.0 AU or more (specified conditions) 2.0 AU or more (specified conditions)

Flow cell Path length: 10 mm, front loading cassette type Path length: 10 mm, front loading cassette type Path length: 10 mm, front loading cassette type

PC communication

USB2.0 USB2.0 USB2.0

Dimensions, weight 300(W) x 470(D) x 150(H) mm, 14.5 kg 300(W) x 470(D) x 150(H) mm, 13.5 kg 300(W) x 470(D) x 150(H) mm, 13.5 kg

Power requirement AC 100 ~ 240 V, 50/60 Hz, 180 VA AC 100 ~ 240 V, 50/60 Hz, 150 VA AC 100 ~ 240 V, 50/60 Hz, 120 VA

Model CD-4095

Light source 150W Hg-Xe lamp

Polarizer Glan-Taylor prism

Phase modulation PEM (photo-elastic modulator)

Wavelength accuracy +/- 5 nm

Wavelength reproducibility +/- 0.5 nm

Wavelength setting range 200 ~ 460 nm

Measurement range 220 ~ 460 nm

Noise level 0.04 mdeg (at specified conditions)

Drift 0.1 mdeg/h (room temperature variation is

within +/-1ºC)

Data output Maximum 100 Hz

Flow cell Front loading cassette cell, tapered, 44 µ

L, 25 mmL

Spectrum measurement 200 ~ 460 nm

Dimensions, weight 300 (W) x 470 (D) x 230 (H) mm, 21 kg

Power requirement AC 100 ~ 240V, 50/60 Hz, 210 VA

Model RI-4030 RI-4035

Measurement system

Refractive index range

Linearity

5.0 x 10

-5

RIU (HIGH)

5.0 x 10

-4

RIU (STD)

5.0 x 10

-3

RIU (LOW) (H2O)

5.0 x 10

-5

RIU (HIGH)

5.0 x 10

-4

RIU (STD) (H2O)

Noise level

0.20 x 10

-8

RIU or less (HIGH, ST D)

0.50 x 10

-8

RIU or less (HIGH, ST D)

Cell capacity 10 µL 2.7 µL

Maximum usable flow-

rate

10 mL/min (Low Flow Rate tubing)

120 mL/min (High Flow Rate

1.2 mL/min (H

Maximum pressure 0.1 MPa (Low Flow Rate tubing)

0.3 MPa (High Flow Rate tubing)

0.1 MPa

Temperature control

Dimensions, weight

Power requirement

Deflection type

1.00 ~ 1.75

Ambient +10ºC ~ ambient +25ºC (setting range: 0 ~ 60ºC)

300(W) x 470(D) x 150(H) mm, 14 kg

AC 100 ~ 240 V, 50/60 Hz, 80 VA

Model AS-4050/AS-4050i AS-4150 AS-4250 AS-4058

Sample injection

method

Full or partially fill loop injection (zero

sample loss available)

Full or partially fill loop injection (zero

sample loss available), direct line

injection (option, change of flow-line

is required.)

Full or partially fill loop injection (zero

sample loss available), direct line

injection (option, change of flow-line

is required.)

Full or partially fill loop injection (zero

sample loss available)

Number of samples 60 (2 mL vial) 180 (2 mL vial) 180 (2 mL vial) 40 (20 mL vial, standard)

Injection volume

0.1 ~ 100 µL

(large volume injection kit: 1 ~ 1000 µ

L, option)

0.1 ~ 100 µL

(large volume injection kit: 1 ~ 1000 µ

L, option)

0.1 ~ 100 µL

(large volume injection kit: 1 ~ 1000 µ

L, option)

1 ~ 10000 µL

Injection precision RSD 0.3 % or less (specified

conditions)

RSD 0.25% or less (specified

conditions)

RSD 0.25% or less (specified

conditions)

SD 1.0 % or less (2500 µL, zero

sample loss)

(specified conditions)

Injection accuracy +/- 0.1% or less (with correction

function)

+/- 0.1% or less (with correction

function)

+/- 0.1% or less (with correction

function)

/- 0.1% or less (with correction

function)

Carry over 0.01% or less

(10 µL, specified conditions)

0.01% or less

(2 µL) (specified conditions)

0.005% or less (with multiple solvent

flushing valve) (specified conditions)

0.01% or less

(2 µL) (specified conditions)

0.005% or less (with multiple solvent

flushing valve) (specified conditions)

.05% or less

(2000 µL, zero sample loss)

(specified conditions)

0.02% or less (with multiple solvent

flushing valve) (specified conditions)

Maximum usable

pressure

30 MPa (AS-4050), 34 MPa (AS-4050i) 70 MPa 130 MPa 41 MPa

Sample

cooling/heating

(option) temp. range: 4 ~ 40ºC (option) temp. range: 4 ~ 40ºC (option) temp. range: 4 ~ 40ºC (option) temp. range: 4 ~ 40ºC

Pre-column

derivatization, etc -

Pre-column derivatization (up to two

reagents), dilution, other user

programs

Pre-column derivatization (up to two

reagents), dilution, other user

programs

Dimensions, weight 300 (W) x 470 (D) x 300 (H) mm, 21 kg

300 (W) x 470 (D) x 385.5 (H) mm, 25 kg

300 (W) x 470 (D) x 385.5 (H) mm, 25 k

Power requirement

AC 100 ~ 240V, 50/60 Hz, 60 VA AC 100 ~ 240V, 50/60 Hz, 75 VA AC 100 ~ 240V, 50/60 Hz, 75 VA AC 100 ~ 240V, 50/60 Hz, 75 VA

Model CO-4061 CO-4062 CO-4060 CO-4065 RO-4068

Temperature range Ambient -15ºC ~ 100ºC Ambient -15ºC ~ 100ºC Ambient -15ºC ~ 80ºC Ambient -15ºC ~ 90ºC Ambient +10ºC ~ 200ºC

Column

compartment

dimensions

280(W) x 25(D) x 60(H) mm 280(W) x 25(D) x 100(H) mm 280(W) x 25(D) x 100(H) mm 110(W) x 83(D) x 415(H) mm 270(W) x 120(D) x 415(H) mm

Safety features

Dimensions, weight 300(W) x 470(D) x 150(H) mm,

10 kg

300(W) x 470(D) x 150(H) mm,

10 kg

150(W) x 470(D) x 465(H) mm,

16 kg

300(W) x 470(D) x 465(H) mm,

25 kg

150(W) x 470(D) x 150(H) mm,

12 kg

Power consumption AC 100 ~ 240 V, 50/60 Hz,

160 VA

AC 100 ~ 240 V, 50/60 Hz,

200 VA

AC 100 ~ 240 V, 50/60 Hz,

350 VA

AC 100 ~ 240 V, 50/60 Hz,

450 VA

AC 100 ~ 240 V, 50/60 Hz,

325 VA

Heating/cooling power shut off when unusually high temperatures or solvent leaks are detected.

Language English/Japanese, selectable

Windows OS Windows 7 Professional 32/64 bit

Windows 8.1 Professional 32/64 bit

Controllable hardware

LC-4000 Series, X-LC 3000 Series, LC-2000 Series,

Maximum 4 systems control, Maximum 20 systems registration

System control Direct control of instrument by Direct Mode,

Time programming by Control Method,

Spectrum measurement,

Prevention from unauthorized use by security key,

Analog signal input,

Up to 4 channel simultaneous input per one LC-Net II/ADC

JASCO INTERNATIONAL CO., LTD.

11-10, Myojin-cho 1-chome, Hachioji, Tokyo 192-0046, Japan

Tel: +81-42-649-3247 Fax: +81-42-649-3518 http://www.jascoint.co.jp/english/

Australia, China, Hong Kong, India, Indonesia, Korea, Malaysia, New Zealand,

Pakistan, Philippines, Russia, Singapore, Taiwan, Thailand

JASCO INCORPORATED

28600 Mary's Court, Easton, MD 21601, U.S.A

Tel: +1-800-333-5272 +1-410-822-1220 Fax: +1-410-822-7526 http://www.jascoinc.com

U.S.A., Canada, Costa Rica, Mexico, Puerto Rico, Argentina, Brazil, Chile, Colombia, Paraguay,

Peru, Uruguay, Guatemala, , Ecuador, Bolivia

JASCO EUROPE s.r.l.

Via Luigi Cadorna 1, 23894 Cremella (Lc), Italy

Tel: +39-039-9215811 Fax: +39-039-9215835 http://www.jasco-europe.com

JASCO Deutschland

www.jasco.de,

JASCO UK

www.jasco.co.uk,

JASCO France

www.jascofrance.fr,

JASCO Benelux

www.jasco.nl,

JASCO Spain

www.jasco-spain.com

Italy, Germany, U.K., France, Netherlands, Belgium, Luxembourg, Spain, Sweden, Norway,

Denmark,

Austria, Finland, Greece, Hungary, Poland, Portugal, Romania, Switzerland, Algeria, Cyprus,

Egypt, Israel, Jordan, Kuwait, Lebanon, Morocco, Saudi Arabia, South Africa, Tunisia,

Turkey, U.A.E., Yemen

LC 4000 0926 Print0

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