Leica Cm1950 Users Manual CM1900_UV_de_1v0

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Operating Manual

Leica CM1950, V 1.1 English – 05/2007

Always keep this manual near the instrument.

Read carefully prior to working with the instrument!

Leica CM1950

Cryostat

3

Leica CM1950 – Cryostat

Published by:

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Phone: +49 (62 24) 143-0

Fax: +49 (62 24) 143-200

eMail: histo_info@leica-microsystems.com

Internet: http://www.histo-solutions.com

These are determined only by the contract provi-

sions agreed between ourselves and our

customers.

Leica reserves the right to change technical spec-

ifications as well as manufacturing processes

without prior notice. Only in this way is it possible

to continuously improve the technology and man-

ufacturing techniques used in our products.

This document is protected under copyright laws.

All copyrights to this documentation are held by

Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of

any parts thereof) by means of print, photocopy,

microfiche, web cam or other methods – includ-

ing any electronic systems and media – requires

express prior permission in writing by

Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of man-

ufacture, please refer to the name plate at the

back of the instrument.

© Leica Biosystems Nussloch GmbH

The information, numerical data, notes and value

judgments contained in this manual represent the

current state of scientific knowledge and state-

of-the-art technology as we understand it follow-

ing thorough investigation in this field. We are

under no obligation to update the present manu-

al periodically and on an ongoing basis accord-

ing to the latest technical developments, nor to

provide our customers with additional copies,

updates etc. of this manual.

For erroneous statements, drawings, technical

illustrations etc. contained in this manual we ex-

clude liability as far as permissible according to

the national legal system applicable in each indi-

vidual case. In particular, no liability whatsoever

is accepted for any financial loss or consequen-

tial damage caused by or related to compliance

with statements or other information in this

manual.

Statements, drawings, illustrations and other

information as regards contents or technical

details of the present manual are not to be

considered as warranted characteristics of our

products.

1. NOTE

4Operating Manual V 1.1 – 05/2007

Table of contents

1. Important Information......................................................................................................................... 6

1.1 Symbols Used in This Manual and Their Meaning ................................................................ 6

1.2 Qualification of Personnel.......................................................................................................... 6

1.3 Designated Use............................................................................................................................ 6

1.4 Instrument Type ........................................................................................................................... 6

2. Safety and Design................................................................................................................................7

2.1 General Safety Instructions ....................................................................................................... 7

2.2 Unpacking and Installation ........................................................................................................ 7

2.3 Safety Features .......................................................................................................................... 10

2.4 Securing/Locking the Handwheel .......................................................................................... 11

2.5 Cleaning, Disinfection – Turning the Instrument Back On.................................................. 12

2.6 Handling Specimens – Defrosting .......................................................................................... 13

2.7 Removal of the Microtome....................................................................................................... 13

2.8 Maintenance .............................................................................................................................. 13

3. Technical Data ................................................................................................................................... 14

4. Standard Delivery .............................................................................................................................. 16

5. General Overview.............................................................................................................................. 18

5.1 Control Panel Fields and Cryostat Chamber.......................................................................... 19

6. Installation ........................................................................................................................................... 20

6.1 Site Requirements ..................................................................................................................... 20

6.2 Transport to the Site ................................................................................................................. 21

6.3 Assembling the Handwheel, Centering the Handwheel (Optional) ................................... 22

6.3.1 Locking/Unlocking the Handwheel ......................................................................................... 23

6.3.2 Installing the Footswitch Dummy (Instruments with Cutting Motor) ................................ 23

6.4 Connection to Power Supply System ..................................................................................... 24

6.5 Installing Accessories/Inserting Chamber Accessories .................................................... 25

6.5.1 Installing the Adjustable Footrest (Optional) ........................................................................ 25

6.5.2 Installing the Storage Systems (Optional) ............................................................................. 26

6.5.4 Inserting the Section Waste Tray ...........................................................................................26

6.5.3 Shelf, Movable (Optional)......................................................................................................... 26

6.5.5 Installing the Heat Conductor Block, Stationary (Optional) ............................................... 27

6.5.6 Installing the Knife/Blade Holder and Adjusting the Clearance Angle............................. 27

5

Leica CM1950 – Cryostat

7. Instrument Controls........................................................................................................................... 30

7.1 Control Panel Fields on the CM1950 – Control Panel Field 1 .............................................. 30

Control Panel Field 2 – Electric Coarse Feed, Sectioning

and Trimming Thickness Display ............................................................................................ 32

Control panel 3 – Motorized sectioning (optional) ............................................................... 34

8. Working with the Instrument......................................................................................................... 36

8.1 Preparing Cutting Tools, Specimen Discs and Preparation Aids ...................................... 36

8.2 Turning the Instrument On ....................................................................................................... 36

8.3 Configuring the Parameters..................................................................................................... 37

8.4 Working with the Precooled Cryostat .................................................................................... 42

8.4.1 Preparatory Work ...................................................................................................................... 42

8.4.2 Trimming with Extraction – Anti-roll Guide Installed ........................................................... 43

8.4.3 Cutting with Extraction – Anti-roll Guide Installed ............................................................... 45

9. Troubleshooting ................................................................................................................................. 48

9.1 Problems During Work ............................................................................................................. 48

10. Temperature Selection Chart (in minus °C) .............................................................................. 52

11. Optional Accessories ....................................................................................................................... 53

11.1 Ordering Information ................................................................................................................. 53

12. Maintenance and Cleaning ............................................................................................................ 66

12.1 General Maintenance ............................................................................................................... 66

12.2 Changing Fuses.......................................................................................................................... 67

12.3 Replacement of the Lamp/UVC Lamp ..................................................................................... 68

13. Decontamination Certificate (master) ........................................................................................ 70

14. EC Declaration of Conformity ........................................................................................................ 72

15. Warranty and Service ...................................................................................................................... 73

16. Peoles Republic of China ................................................................................................................ 74

6Operating Manual V 1.1 – 05/2007

1. Important Information

1.1 Symbols Used in This Manual and

Their Meaning

Notes

i.e. important user information appear

in a gray box and are marked by an in-

formation symbol.

Caution – UVC radiation!

Figures in brackets refer to item num-

bers in drawings or to the drawings

themselves.

(5)

(Fig.5)

Warnings

appear in a gray box and are marked

by a warning triangle.

1.4 Instrument Type

All information given in this instruction manual

applies only to the instrument type indicated on

the title page.

A name plate, indicating the instrument serial

number, is attached to the back of the instrument.

Fig. 1

1.3 Designated Use

The CM1950 is a high-performance cryostat with

an encapsulated microtome and separate speci-

men cooling. It features a UV disinfection system,

an (optional) integrated extraction system for sec-

tion waste and an (optional) motor for motorized

sectioning.

The cryostat is designed to produce frozen sections

for biological, medical and industrial applications.

The CM1950 is suitable for in-vitro diagnostic

(IVD) applications.

The instrument may only be operated within the

scope of its designated use as described above

and as per the instructions given in this manual.

Any other use of this instrument is considered

as improper operation.

Despite chemical and/or UV-light dis-

infection, personal safety precautions

as per the applicable laboratory regu-

lations must still be taken (i.e. safety

goggles, gloves, laboratory coat and

mask must be worn).

This type of disinfection reduces the

number of germs by at least 99.99%.

1.2 Qualification of Personnel

The Leica CM1950 may be operated by trained

laboratory personnel only.

All laboratory personnel designated to operate

the instrument must carefully read the present

instruction manual prior to starting work with the

instrument.

7

Leica CM1950 – Cryostat

2.1 General Safety Instructions

The CM1950 is a cryostat with an encapsulated microtome and separate

specimen cooling. It is primarily used for work in the area of fast-cut diag-

nostics. The displays and instrument controls are easy to operate due to

their largely self-explanatory symbols. LED displays make it easy to read.

The cryochamber is made of seamlessly welded, high-quality stainless steel

that is free of difficult-to-access corners and thus easy to clean and disinfect.

This instrument has been built and tested in accordance with the following

safety regulations on electrical measuring, control, regulating and labora-

tory devices. In order to maintain this condition and to ensure safe opera-

tion, the operator must observe the instructions and warnings contained in

this instruction manual.

For current information about applicable standards, please refer to the

CE declaration of conformity on our Internet site:

www.histo-solutions.com

2.2 Unpacking and Installation

• To ensure proper function of the instrument, it must be set up with a

minimum distance on all sides from walls and furniture (see ‘Site

Requirements’, p. 20)

• The instrument may only be transported in an upright or slightly inclined

position.

• To ensure a safe transport with a fork lift 3 people are required: one op-

erating the fork lift, and the other 2 holding the instrument on either

side to prevent it from sliding down.

• Before connecting to the power supply system, please check if the local

voltage complies with the power rating specified on the name plate of

the instrument (see also ‘Technical data’)!

• Never connect the instrument to a power socket that does not have a

protective conductor terminal.

Length of power cable: up to 3.5 m extension possible: no

After transporting, wait at least 4 hours before turning the instrument on. This waiting period is

necessary to allow the compressor oil, which may have been displaced during transport, to return

into its original position. Any condensation on electrical parts that has formed due to tempera-

ture differences during transport must be allowed to dry completely. Failure to comply with this

can cause severe damage to the instrument!

2. Safety and Design

8Operating Manual V 1.1 – 05/2007

2. Safety and Design

Opening the packaging

1. Remove the 3 screws (1) on each of the two sides of the

box (7) at the top.

2. Remove the 2 screws on each of the 4 hinges (2) on the

front and rear side of the box.

Do NOT remove the screws of the hinges on the top

lid!

3. Remove 1 screw (3) between the two hinges on the front

and rear side of the box.

4. Remove the 3 screws (4) at the bottom on all four sides

of the box.

5. Of the two vertical rows of screws (5), remove one on

either side (which side does not matter) entirely.

22

7

13

When the instrument is delivered, check the tilt indicators on the

packaging.

If the arrowhead is blue, the shipment was transported laying flat,

was tilted at too great an angle or fell over during transport.

Note this on the shipping documents and check the shipment for

possible damage.

Fig. 2

4

5

5

5

4

8

8

8

8

8

17

9

9

Cover = ramp

10

14

11

11

Fig. 3

9

Leica CM1950 Cryostat

2. Safety and Design

Removing the packaging

1. Remove the cover (17) and place it on the floor with the foam parts facing

downwards. - The cover will be used as a ramp later.

2. Remove the 4 foam parts (6) by pulling them upwards.

3. Open the box (7) on its side hinges like an accordion partition and remove

it.

4. Remove the accessories carton (8) and operating manual on the front of

the instrument.

5. Pull off the dust cover (9) by pulling upwards.

6. Take off the foam strip (10) on the bottom rear.

Ramp assembly

1. After the foam strip (10) is removed, 2 screws (12) are visible on the

baseplate.

2. Swing out the cover hinges (2) at the front and rear.

3. Attach the cover (17) from the rear on the transport pallet (11).

The notches (13) of the hinges (2) must be pointing towards the instrument.

4. Move the cover towards the left so that the notches (13) of the hinges

slide beneath the heads of the screws (12).

Transport to the Installation Location

The instrument must be transported in an upright position.

The cover (17) now forms a ramp on which the instrument can now be rolled

down off the transport pallet.

Do not push the device by its hood (16). Use the transport grip

points (15 ).

The rollers (14) must run over the cover hinges (2) at the front and

rear. Danger of tipping!

1. Carefully roll the instrument backwards from the pallet.

2. Push the instrument to the installation location on the castors (14).

13

12

6

66

6

17

16

14

22

Fig. 5

Fig. 4

Fig. 6

15

10 Operating Manual V 1.1 – 05/2007

To prevent adverse health effects from UV radiation, the UV disinfec-

tion cycle can be started only after the sliding window has been prop-

erly closed. Closing the window activates the corresponding safety

features.

The consistent use of these safety features and strict observation of the warn-

ings and cautions in this manual, will safeguard the operator from accidents

and/or personal injury to a great extent.

Microtome knives

• Take care when handling microtome knives/disposable blades. The

cutting edge is extremely sharp and can cause severe injury!

• Never leave knives and dismounted knife holders with a knife/blade

mounted lying around!

• Do not place a knife on a table with the cutting edge facing upwards!

We strongly recommend using the safety gloves included with the

standard delivery!

•Never try to catch a falling knife!

• Prior to manipulating the knife and specimen or changing the specimen,

always lock the handwheel and cover the cutting edge with the knife

guard!

• Avoid contact with cold parts of the instrument as this can cause frostbite!

2. Safety and Design

2.3 Safety Features

The instruction manual includes important information related to the operating

safety and maintenance of the instrument.

The Operating Manual is an important part of the product, and must be read

carefully before startup and use and must always be kept near the instrument.

If additional requirements on accident prevention and environmental

protection apply in the country of operation, this Operating Manual must be

supplemented by appropriate instructions to ensure compliance with such

requirements.

The instrument is equipped with the following safety devices: an emergen-

cy stop switch (motorized instruments only), a handwheel lock and center-

ing system (motorized instruments only), knife guard on the blade and knife

holder, and a blade ejector.

11

Leica CM1950 – Cryostat

2.4 Securing/Locking the Handwheel

Always lock the handwheel and cover the cutting edge with the

knife guard prior to manipulating the knife or specimen, changing

the specimen, or taking a break.

To lock the handwheel, press the lever (1) outward. Continue turning the

handwheel slowly until the grip is in the upper or lower position and the

handwheel is locked. Press the lever fully outward; gently rock the hand-

wheel until the locking mechanism clicks into place noticeably.

To release the handwheel, press the lever (2) on the handwheel toward the

cryostat housing.

Centering of the handwheel (motorized instruments only)

Pull out the handwheel’s handle and position it in the middle of the

handwheel. The handle automatically engages in this position.

An important safety device on the cryostat is the centering of the

handwheel for motorized instruments.

Rotate the handwheel only if the refrigeration system is on and the

cryochamber is cold.

12 o’clock

position

6 o’clock

position

1

2

Fig. 8

Fig. 7

Fig. 9

2. Safety and Design

Knife guard

Prior to manipulating the knife and specimen, changing the speci-

men or knife, or taking a break, always lock the handwheel and

cover the cutting edge with the knife guard.

The CE, CN and CN-Z knife holders feature knife guards; the glass anti-roll

plate of the CE knife holder also serves as a knife guard.

12 Operating Manual V 1.1 – 05/2007

2. Safety and Design

• When disinfecting the instrument, take appropriate protective measures

(gloves, mask, protective clothing, etc.).

• When using detergents and disinfectants please comply with the safety

precautions of the disinfectant manufacturer.

• The integrated glass anti-roll guide of the blade holder CE, CN and CN-Z

can be cleaned either with acetone or alcohol.

• Dispose of waste liquid according to the waste disposal regulations!

• Do not use external heaters for drying the cryochamber. This can cause

damage to the cooling system!

• Do not turn the instrument on before the cryochamber is completely dry!

Frost formation!

• All components removed from the cryostat must be carefully dried be-

fore returning them to the cryochamber!

• The front panel and the slit cover of the microtome must be completely

dry before turning on the instrument!

For more detailed information about disinfection, visit the Leica

Microsystems website at

www.histo-solutions.com

It is not necessary to remove the microtome for disinfection.

• The instrument has been designed for UV disinfection! Spray disinfec-

tion with Leica Cryofect is also possible, thanks to the special insulation

of the microtome. (Cryofect is not available in all countries!)

Remove section waste after EVERY sectioning operation and BE-

FORE changing specimens. Remove the section waste using a pa-

per towel soaked in Cryofect or an alcohol-based disinfectant) or

remove the waste using the extraction nozzle (optional). Do not

start the disinfection until after folding the section stretcher to the

side! Each new specimen is a potential source of contamination.

2.5 Cleaning, Disinfection – Turning the Instrument Back On

13

Leica CM1950 Cryostat

2. Safety and Design

2.7 Removal of the Microtome

• The microtome is encapsulated and therefore does not require removal

by the user.

2.8 Maintenance

Replacing the fuses

• Turn the instrument off and disconnect the power plug before replacing

the fuses.

• Use only the fuse types specified in chapter 3, "Technical Data." Failure

to comply with this can cause severe damage to the instrument!

Replacing the fluorescent lamp/UVC lamp

• Turn the instrument off and disconnect the power plug before replacing

the lamps.

It is possible to break the UVC lamp during replacement. If this

happens, the lamp change must be completed by Technical Ser-

vice. If any metallic mercury is released, handle it carefully and

dispose of it properly.

If both disinfection indicator lights are blinking alternately, the

UV tube must be replaced!

2.6 Handling Specimens – Defrosting

• When working with contaminated or infected material, the general safety

guidelines for laboratories must be applied.

• Before defrosting the cryochamber remove all samples!

• Before defrosting the specimen head, remove all samples!

Never leave samples in the cryochamber! - The instrument is not

suitable for storing frozen specimens, as the refrigeration dehy-

drates the specimens!

The quick freeze shelf can become very hot during the defrosting

process. Do not touch it during defrosting.

14 Operating Manual V 1.1 – 05/2007

3. Technical Data

②according to IEC-1010; UL 3101

Type

Nominal voltage (±10 %)

Nominal frequency

Power draw

Max. start-up current for 5 sec.

Protective class

Circuit breaker:

(MDA made by Schurter)

Pollution degree②

Overvoltage installation category

Heat emission (max.)

Approval

-1 -2 -3

100 VAC 120 VAC 230 VAC

50/60 Hz 60 Hz 50 Hz

1900 VA 1900 VA 1900 VA

35 A eff. 35 A eff. 25 A eff.

III

15A M3 15A M3 15A M3

222

II II II

1900 J/s 1900 J/s 1900 J/s

CE/c_CSA_us CE/c_CSA_us CE/c_CSA_us

All specifications related to temperature are valid only up to an ambient temperature of 18°C

to 35°C and a relative humidity of no more than 60%.

Microtome

Type: Rotary microtome,

encapsulated

Section thickness range 1 to 100 μm

Trimming range:

Clinic 10 - 40μm ③

Research 1 - 600 μm ③

Total specimen feed 25 mm + 1 mm

Vertical stroke 59 mm ±0.5mm

Specimen retraction 20μm (can be deactivated)

Maximum specimen size 50 x 80 mm

Cutting speed Slow: 0-50 strokes/min

Fast: 0-85 strokes/min

Vmax: 85-90 strokes/min

Specimen orientation 8° (x, y-axis)

Electric coarse feed

Slow 300μm/s

Fast 900 μm/s

Cryostat

Dimensions:

Width (w/o handwheel) 700 mm

Width (with handwheel) 835 mm

Depth (cabinet only) 850 mm

Height (total) 1215 mm

Working height (armrest) 1025 mm

Weight:

Weight (w/motor and suct.) 193 kg

Weight (w/motor, w/o suct.) 185 kg

Weight (w/o motor, w/ suct.) 183 kg

Weight (w/o motor, w/o suct.) 175 kg

Weight (w/ specimen head cool.) 165 kg

Weight (w/o specimen head cool.) 145 kg

For "Site Requirements," refer to item 6.1

(page 20).

Lamp

50/60 Hz-Version: Osram DULUX L 18W/840

General information

Operating temperature range: 18 °C to 35°C

Temperature range

during storage: +5 °C to +55 °C

Relative humidity: max. 60 %,

non-condensing

Storage humidity: < 60 %

③ For additional details, see p. 32

15

Leica CM1950 – Cryostat

3. Technical Data

Refrigeration system CM1950, 50 Hz CM1950, 60 Hz

Cryochamber

Temperature range 0°C to -35°C ± 5 K, adjustable in 1K increments, 0°C to -35°C ± 5 K, adjustable in 1K increments

at ambient temperature of 20°C ambient temperature of 20 °C

Cooling time to -25°C approx. 5 h approx. 5 h

Cooling time to -35°C approx. 8 h approx. 8 h

Refrigeration capacity ①690 W 690 W

Cut-out pressure 25 bar 25 bar

Safety factor 3 3

Refrigerant* 300 g (± 5 g) refrigerant R -404A* 300 g (± 5 g) refrigerant R -404A*

Compressor oil* 0.6 l EMKARATE RL22S, ICI* 0.6 l EMKARATE RL22S, ICI*

Defrosting of cryochamber

Automatic defrosting

Programmable: Yes (hot gas defrost) Selectable time Yes (hot gas defrost) Selectable time

Defrosting intervals 1 defrost in 24 h 1 defrost in 24 h

or manual hot gas defrost or manual hot gas defrost

Defrost time: 12 minutes 12 minutes

Automatic shutoff Defrost: at -5 °C chamber temperature at -5 °C chamber temperature

Quick-freeze shelf

Minimum temperature: - 42 °C (+ 5 K), at chamber temp. -35°C - 42 °C (+ 5 K), at chamber temp. -35°C

Number of freezing stations: 15+2 15+2

Defrost Manual hot-gas defrost Manual hot-gas defrost

Peltier element

Number of freezing stations: 2 2

Max. temperature difference: 17 K, at chamber temp. of -35°C 17 K, at chamber temp. of -35°C

Specimen cooling

Temperature range -10 to -50 °C ± 3 K -10 to -50 °C ± 3 K

Refrigeration capacity①320 W 320 W

Cut-out pressure 25 bar 25 bar

Safety factor 3 3

Refrigerant and quantity at 230V/50 Hz 140 g (± 5 g), refrigerant R-404A* at 120V/60 Hz 110 g (± 5 g), refrigerant R-404A*

at 100V/50/60 Hz 110 g (± 5 g), refrigerant R-404A*

Compressor oil* 0.4 l alpha 22, Kyodo* 0.4 l alpha 22, Kyodo*

Defrosting of specimen head

Automatic defrost: No No

Manual defrost

Defrost time 15 min. 15 min.

*) Refrigerant and compressor oil must be replaced by qualified, authorized service personnel only.

① acc. to CECOMAF: liquid temperature 45 °C, evaporation temperature: -25 °C

16 Operating Manual V 1.1 – 05/2007

4. Standard Delivery

A choice of different knife holders is available for the CM1950.

Compare the delivered components with the parts list and your order. Should you

find any discrepancies, please contact your Leica sales office without delay.

Basic instrument WITHOUT motor/WITHOUT extraction, in the specific voltage variant

1 handwheel, manual ......................................................................................... 0477 41346

5 specimen discs, 30 mm ................................................................................... 0477 40044

1 section waste tray ........................................................................................... 0477 40062

1 position holder for freeze shelf ..................................................................... 0477 40080

1 freeze shelf cover ............................................................................................ 0477 43763

1 tool set ............................................................................................................... 0436 43463

- 1 brush, fine ................................................................................................ 0183 28642

- 1 Leica brush w/magnet ........................................................................... 0183 40426

- 1 Allen key, No. 1.5 ..................................................................................... 0222 10050

- 1 Allen key, No. 2.5 ..................................................................................... 0222 04137

- 1 Allen key, No. 3.0 ..................................................................................... 0222 04138

- 1 Allen key, No. 4.0 ..................................................................................... 0222 04139

- 1 Allen key with dome head, No. 4.0 ....................................................... 0222 32131

- 1 Allen key, No. 5.0 ..................................................................................... 0222 04140

- 1 key with handle, No. 5.0 ......................................................................... 0194 04760

- 1 Allen key, No. 6.0 ..................................................................................... 0222 04141

- 1 Single-head wrench, No. 13 ................................................................. 0330 33149

- 1 Single-head wrench, No. 16 ................................................................. 0330 18595

1 power cable .........................................................................................................................

1 bottle of cryostat oil, type 407, 50 ml ............................................................ 0336 06098

1 bottle of OCT freezing compound, 125 ml .................................................... 0201 08926

1 pair of safety gloves, size M, * for cryosectioning .................................... 0340 29011

1 user manual plus language CD ..................................................................... 0708 37115

* Note: for the Japanese version: 100V, 50/60 Hz; 1 pair of safety gloves, size S

(0340 40859) is included.

Basic instrument WITHOUT motor and WITH extraction,

Standard scope of delivery as above,

plus:

1 accessory set (extraction) ............................................................................. 0477 43300

- Hose adapter 1 ........................................................................................... 0477 40293

- Hose adapter 2 ........................................................................................... 0477 40294

- Suction nozzle ............................................................................................ 0477 40295

- Silicone hose .............................................................................................. 0477 43302

- Silicone stopper ......................................................................................... 0477 43304

- Chamber suction nozzle ........................................................................... 0477 43779

- Set of filters (5 pieces) .............................................................................. 0477 43792

17

Leica CM1950 – Cryostat

4. Standard Delivery

Basic instrument WITH motor/WITHOUT extraction, in the specific voltage variant

1 handwheel, motorized .................................................................................... 0477 41347

5 specimen discs, 30 mm ................................................................................... 0477 40044

1 section waste tray ........................................................................................... 0477 40062

1 position holder for freeze shelf ..................................................................... 0477 40080

1 freeze shelf cover ............................................................................................ 0477 43763

1 tool set ............................................................................................................... 0436 43463

- 1 brush, fine ................................................................................................ 0183 28642

- 1 Leica brush w/magnet ........................................................................... 0183 40426

- 1 Allen key, No. 1.5 ..................................................................................... 0222 10050

- 1 Allen key, No. 2.5 ..................................................................................... 0222 04137

- 1 Allen key, No. 3.0 ..................................................................................... 0222 04138

- 1 Allen key, No. 4.0 ..................................................................................... 0222 04139

- 1 Allen key with dome head, No. 4.0 ....................................................... 0222 32131

- 1 Allen key, No. 5.0 ..................................................................................... 0222 04140

- 1 key with handle, No. 5.0 ......................................................................... 0194 04760

- 1 Allen key, No. 6.0 ..................................................................................... 0222 04141

- 1 Single-head wrench, No. 13 ................................................................. 0330 33149

- 1 Single-head wrench, No. 16 ................................................................. 0330 18595

1 power cable .........................................................................................................................

1 bottle of cryostat oil, type 407, 50 ml ............................................................ 0336 06098

1 footswitch dummy ........................................................................................... 0443 30420

1 bottle of OCT freezing compound, 125 ml .................................................... 0201 08926

1 pair of safety gloves, size M, * for cryosectioning .................................... 0340 29011

1 operating instructions plus language CD .................................................... 0708 37115

* Note: for the Japanese version: 100V, 50/60 Hz; 1 pair of safety gloves,

size S (0340 40859) is included.

Compare the delivered components with the parts list and your order. Should you

find any discrepancies, please contact your Leica sales office without delay.

Basic instrument WITH and WITH extraction, in the specific voltage variant

Standard scope of delivery as above,

plus:

1 accessory set (extraction) ............................................................................. 0477 43300

- Hose adapter 1 ........................................................................................... 0477 40293

- Hose adapter 2 ........................................................................................... 0477 40294

- Suction nozzle ............................................................................................ 0477 40295

- Silicone hose .............................................................................................. 0477 43302

- Silicone stopper ......................................................................................... 0477 43304

- Chamber suction nozzle ........................................................................... 0477 43779

- Set of filters (5 pieces) .............................................................................. 0477 43792

A choice of different knife holders is available for the CM1950.

18 Operating Manual V 1.1 – 05/2007

5. General Overview

Castors for safe transport

over short distances

Handwheel in

12 o’clock

position

On/off switch, also circuit

breaker

Align adjusting feet for secure

upright position

Unscrew and align

adjusting feet after

transport

Bacterial filter

(optional, only for

instruments with filter)

Catch tank for

condensate

Footswitch dummy

(motorized

instruments only)

Emergency-stop

switch (motorized

instruments only)

Cryostat chamber

(with activated

UV disinfection)

Fig. 10

19

Leica CM1950 – Cryostat

5. General Overview

5.1 Control Panel Fields and Cryostat Chamber

1

23

1- Control panel field 1: Extraction, temperature and time control, illumination, UV disinfection

2- Control panel field 2: Electric coarse feed (sectioning and trimming thickness adjustment)

3- Control panel field 3: Motorized sectioning cutting, optional (adjustment of stroke type, cutting speed etc.)

4- Heat conductor block, stationary (optional)

5- Peltier element (with 2 stations)

6- Freeze shelf, 15 positions

7- Position holder on freeze shelf

8- Heat and cold extractor, mobile (opt.)

9- Shelf, movable (optional)

10 - Blade holder CE with blade ejector (a)

10b- Finger rest on the blade holder CE

4

10a

9

8

615

5

10c- Knife guard on the blade holder CE

11 - Extraction nozzle on the extraction hose

12 - Extraction hose for section waste

13 - Brush shelf (optional)

14 - Adapter piece for extraction hose

(the coarse filter insert is behind it)

15 - Object head, directional

16 - Waste tray

13

10b 11

12

10 14

Fig. 11

7

10c

16

20 Operating Manual V 1.1 – 05/2007

6. Installation

6.1 Site Requirements

The place of installation must meet the following requirements:

• No direct sunlight.

• Power supply at a distance no greater than approx. 3 m.

• No drafts (air condition outlets etc.).

• Even floor.

• Vibration-free floor.

• The instrument is designed for indoor use only.

• Obstruction-free access to the handwheel.

• The power switch/circuit breaker must be freely and easily accessible.

• Room temperature always approx. 22 °C.

• The relative humidity must not exceed 60%.

To ensure proper function of the instrument, it must be set up while

maintaining a minimum distance from walls and furniture.

• Distance to walls and furniture, calculated from the cabinet:

- rear: 15 cm

- right side: 30 cm

- left side: 15 cm

• No heat dissipating appliances around.

High room temperatures and excessive air humidity affect the

cooling capacity of the cryostat and lead to ice forming in the

instrument!

• Plug the instrument into power sockets with ground only.

Power cable length: up to 3.5 m

Extendable: no

21

Leica CM1950 – Cryostat

6. Installation

6.2 Transport to the Site

• First, check if the location meets the conditions specified in "Site

requirements".

• Transport the instrument to the desired location.

• Observe the following:

The instrument must be transported in an upright position or slight-

ly tilted (max. 30°)!

• When transporting the instrument on wheels

(2) grip the cabinet only at the marked loca-

tions ( ).

• To do so, unscrew the adjustable feet using

the No. 13 open-end wrench (when subse-

quently transporting the instrument on cas-

tors, screw the feet back in as far as they will

go). To ensure a secure upright position at its

intended location, align both adjusting feet (1).

1

When tilting the instrument 2 people must counterbalance from the

front side to prevent the instrument from falling down and causing

severe injury!

1

2

2

2 (covered)

Fig. 12

Before transport or relocation, remove

the filter bag from the chamber. If you

fail to do this, the filter bag will thaw,

then freeze solid when the instrument

is reconnected. When subsequently

removed, the filter will be destroyed,

causing section waste to get into the

bacteria filter. (Refer to p. 26.)

When not using the extraction for a long

period, tightly close the opening for the

extraction hose using the silicone stop-

per (5, page 28) included in the scope of

delivery.

22 Operating Manual V 1.1 – 05/2007

• Insert the pin (1) of the handwheel shaft into

the hole (2) of the handwheel.

• Tighten the screw (4) using the No. 6 Allen key.

• Place the protective cap on the screw (4).

To dismount, proceed in reverse order.

1

24

6.3 Assembling the Handwheel

Fig. 14

Transport with a fork lift

• The instrument can be transported with a fork lift.

To ensure a safe transport with a fork lift 3 people are required:

one operating the fork lift, and the other 2 holding the instrument

on either side to prevent it from sliding down.

• At the installation site, unscrew the adjustable feet (1) with the open-

end wrench No. 13 (see Fig. 12). This is absolutely necessary for a stable

stand.

Fig. 13

Total width with handwheel

6. Installation

Rotate the handwheel only if the

refrigeration system is on and the

cryochamber is cold.

23

Leica CM1950 Cryostat

6.3.1 Locking/Unlocking the Handwheel

Only rotate the handwheel when the refrigeration system is on and

the cryochamber is cold!

Always lock the handwheel prior to manipulating the knife or

specimen, changing the specimen, or taking a break.

To lock the handwheel, move its handle to the 12 or 6 o’clock position. Press

the lever (1) fully outward; gently rock the handwheel until the locking mech-

anism clicks into place noticeably.

To release the handwheel, press the lever (2) on the handwheel toward the

cryostat housing.

12 o’clock

position

6 o’clock

position

1

2

Fig. 16

Fig. 15

6. Installation

6.3.2 Installing the Footswitch Dummy (Instruments with Cutting Motor)

• The footswitch dummy must be installed on the outer right side of the

instrument (see page 16, General overview) if no footswitch (optional) is

used.

If the red LED in the E-STOP field in control panel field 3 is illuminated, either:

• emergency-stop function active, or

•

footswitch dummy (optional footswitch) connected incorrectly or not at all.

Fig. 18

Fig. 17

An important safety device on the cryostat is the feature for center-

ing the handwheel in motorized sectioning mode.

To do so, pull out the handwheel’s handle and position it in the middle of the

handwheel. The handle automatically engages in this position.

Centering the Handwheel (Optional)

24 Operating Manual V 1.1 – 05/2007

6.4 Connection to Power Supply System

After transporting, wait at least 4 hours before turning the instrument on. This waiting period is

necessary to allow the compressor oil, which may have been displaced during transport, to re-

turn into its original position. Furthermore, any condensation that has formed during this time

due to temperature fluctuations must be allowed to dry completely.

Failure to comply with this can cause severe damage to the instrument!

During the start-up of the compressor the nominal voltage must not drop below the values

specified in the "Technical Data".

Please note that the compressor requires a start-up current between 25 and 35 A.

Therefore, the electric circuit at the installation site must be inspected by an electrical

engineer to ensure that it meets the requirements for a smooth operation of the instrument.

Failure to comply with the above will cause severe damage to the instrument!

• Check mains voltage and mains frequency to comply with the specifica-

tion on the type plate.

• Do not connect any other consumers to this electric circuit.

Never connect the instrument to a power socket that does not have a protective conductor

terminal.

6. Installation

Fig. 19

Power plug

Only for instruments sold in Japan

1

Selecting the frequency

• After unpacking the instrument and setting it up at its intended location,

use the lever (1) to select the frequency corresponding to the conditions of

the existing power system.

25

Leica CM1950 – Cryostat

6.5 Installing Accessories/Inserting Chamber Accessories

6.5.1 Installing the Adjustable Footrest (Optional)

• To install the optional footrest, unscrew the

screws (1) using the No. 3 Allen key provided.

1

• Attach the holders (2) for the footrest on the

left and right to the front wall of the housing.

To do so, use the Allen screws you used earli-

er. Ensure that the screws are tight.

• Hook the footrest (3) into the installed holder

according to individual requirements (height).

• Once installed, the user can adjust the height

of the footrest at any time by relocating it (3)

to the desired height on both sides in the

holder (2).

2

3

Fig. 20

6. Installation

When installing the holder (2), ensure

that the cutout faces downwards so that

the support (3) can be hooked in.

26 Operating Manual V 1.1 – 05/2007

6.5.2 Installing the Storage Systems (Optional)

6.5.3 Shelf, Movable (Optional)

Attach the rod for the shelf to the inner front side

of the cryostat housing using the provided screws

(1) and the No. 3 Allen key, then attach the caps

(3). (The rear side of the movable shelf has white

plastic screws (2) that prevent the interior of the

chamber from being scratched.) Now hook the

movable shelf into the guide rod.

6.5.4 Inserting the Section Waste Tray

Fig. 22

6. Installation

To do so, remove the insert (1), place the frame

(2) in front of the bore and tighten the screws/

washers on the cryostat housing using the No. 4

Allen key. Afterwards, insert the insert (1) into the

frame and fold it up.

Fig. 23

Before installing the knife/

blade holder base, insert the

section waste tray with the

cutout (a) facing the user.

a

1

2

Fig. 21

For reasons of

accessibility, the

(optional) storage

system must always

be installed first.

1

2

3

27

Leica CM1950 – Cryostat

6. Installation

6.5.5 Installing the Heat Conductor Block, Stationary (Optional)

Fig. 24

6.5.6 Installing the Knife/Blade Holder

and Adjusting the Clearance Angle

For temperature reasons, install the

knife/blade holder on an appropriate

base.

• Set the knife or blade holder (1, Fig. 25) on the

base (2), adjust the clearance angle (on the

left of the knife/blade holder) to approx. 2°-5°

and secure it in the bore (3) on the base (2)

using the No. 4 Allen key.

• Push the knife holder base (2) on the dovetail

guide (4) from the front and tighten it using the

clamping lever (5). To move the base, open the

clamping lever only a little to prevent acciden-

tal sliding in the direction of the specimen

head.

• If the clamping distance is not sufficient, the

clamping lever (3) can be moved. To do so, pull

the lever out and move it to the next position.

The holder (1, Fig. 24) of the heat conductor block

is screwed to the left housing wall using the

No. 4 Allen key provided. (Preferably, begin with

the bottom screw. Then, rotate the holder

upwards (see arrow) and insert and tighten the

top screw).

1

Fig. 25

4

When removing the knife holder base

(2) from the refrigerated cryostat

chamber, hold it by the grip points

(a – front and rear) to keep your

fingers from freezing.

Now, attach the cover for the quick

freeze shelf to protect the shelf from

frost.

1

2

3

2a

5

28 Operating Manual V 1.1 – 05/2007

Reasons:

1. To prevent section waste from falling into the

opening.

2. To prevent cold from escaping from the

chamber.

3. To prevent moisture from penetrating into the

chamber.

6. Installation

6.5.7 Inserting/Changing the Bacteria Filter

6.5.8 Assembling the Filter Bag

Fig. 27

• Set the mark (A) of the extraction opening (1)

and pull it out. Plug the filter (3) into the ex-

traction hose connecting piece (3a) until there

is an audible click.

Now push the connected parts back into the

opening in the cryostat chamber (filter first) and

set it to the "closed" mark (2, Fig. 27).

1

3

3a

2

The holder for the bacterial filter (optional) is

visible in the front of the instrument.

• To insert the filter, hold it with one hand, press

on the right of the socket, then guide the filter

into the tube from the left.

• To change the filter, follow the opposite proce-

dure: press the filter to the right, then pull it to

the left and out of the tube.

• The filter must be changed approx. every

3 months (we recommend writing the date on

the filter using a marker).

Fig. 26

Fig. 28

When not using the extraction, tightly

close the opening for the extraction

hose using the silicone stopper (E) in-

cluded in the scope of delivery.

A

E

The filter must be disposed of accord-

ing to valid laboratory specifications.

L

29

Leica CM1950 – Cryostat

6.5.9 Installing the Section Extraction (Optional) – Use with Blade Holder CE Only

6. Installation

If the extraction is not used for a long time, it is absolutely necessary to clean the extraction hose in order

to ensure maximum extraction capacity. To do so, place the hose in commercially available disinfectant or

alcohol. After several cleanings, the hose must be replaced (see order information on p. 49)!

When the suction nozzles are changed, the adapt-

er (white) remains in the silicone hose. Pull off

the nozzle by rotating and pulling it gently and

firmly plug in the desired nozzle.

A

D

I

B

C

Fig. 29

A

B

CD

F

E

H

G

Mark for

suction

nozzle

If the extraction nozzle (D) is not being

used, it can be "parked" on one of the

two magnetic surfaces indicated in the

interior of the instrument.

Ensure that the hose with the nozzle is

not installed against its "natural" cur-

vature on the pressure plate of the

blade holder.

The tension acting on the hose can be minimized

by turning the red ring (Fig. 31, top right) clock-

wise so that the suction nozzle presses against

the pressure plate (I, Fig. 31).

Afterwards, fold the anti-roll guide (K) back onto

the pressure plate.

Fig. 30

Fig. 31

K

• The scope of delivery also includes 2 plastic

clips (H). These enable comfortable "parking"

of the chamber suction nozzle (F) during sec-

tioning.

The clip must be glued in before switching on the

refrigeration. Before doing so, briefly degrease

the surface to ensure a secure hold.

Preferably, the clip should be attached outside

the working area, e.g. on the left inside wall of

the instrument.

• Silicone hose (A) with hose adapter 1 (B, to the filter

in the instrument), hose adapter 2 (C, for suction

nozzle D or F) and suction nozzle (D) – factory pre-

assembled

• Silicone stopper (E)

• Chamber suction nozzle (F)

• Filter (G)

• Plastic clip (H), for parking the chamber suction

nozzle.

30 Operating Manual V 1.1 – 05/2007

7. Instrument Controls

Display of actual and target specimen

head temperatures

Display of actual and target chamber

temperatures

Display of real time, defrost time

and error messages

Max-Cool button to

select the maximum

low temperature of

the specimen head

directly (-50 °C)

+/- buttons to

select

specimen

head

temperature

+/- buttons to

select

chamber

temperature

+/- buttons to

select defrost

time

+/- buttons to

select real

time

Press the key button to

lock/unlock the entire

keypad.

(Refer to following page

for activation of the

specimen head)

"Melting

Snowflake"

button to

activate manual

defrost

ON/OFF button of

illumination

7.1 Control Panel Fields on the CM1950 – Control Panel Field 1

Button to

enable/disable

the Peltier

element

UVC

disinfection,

(short time 30min,

long time 180 min)

Button to

enable/disable

vacuum

extractor,

Extraction

force intensity

selectable

from 1-5 (see

following page

for more

information)

EMERGENCY STOP switch to the right of control panel field 1 (motorized

instruments only)

For danger situations during motorized sectioning.

•Immediate stop of the sectioning process – motor stops – LED in E-Stop is

illuminated in red.

• Turning in direction of arrow cancels the stop – LED in E-Stop goes out.

• Select single stroke (Single) or continuous stroke (Cont.) operating

mode again.

Fig. 32

31

Leica CM1950 Cryostat

• The Peltier element provides additional cooling for the freezing stations.

After the button is pressed, the display changes from "PE" to 10",

indicating an additional cooling period of 10 minutes. The remaining cool-

ing time is always shown in the display. Once only 4 minutes are remain-

ing, a dot will appear after the "4". As of this time, the Peltier element may

be switched off early by pressing the button again.

Caution:

The specimen head and Peltier do not switch on until the chamber

temperature reaches -5°C, in order to prevent icing.

• Press the "VAC" button to enable the vacuum extractor. The LED in the

"VAC" button is lit while the extractor is on. Press the button again to

disable it.

• Use the knob to adjust the intensity of the vacuum.

A Optimal area for trimming and sectioning

• Trimming: Handwheel position 12 - 6 o’clock, valve open

Handwheel position 6 - 12 o’clock, valve closed

• Cutting: Handwheel position 12 - 3 o’clock, valve open all the way

Handwheel position 3 - 6 o’clock, valve half open

Handwheel position 6 - 12 o’clock, valve closed

B Optimal area for extraction from the chamber

• To clean the chamber, turn the knob to the red range.

7. Instrument Controls

Position

12 o’clock

6 o’clock

A

B

The strength of the required extraction force depends on the following:

• Size of the specimen

• Sectioning speed

• Section thickness used

(For exact instructions for using the chamber, specimen head and

real time display fields, refer to the chapter on "Working with the

Instrument" on page 37 of this Operating Manual.)

If the condenser (resting phase) is off and the Peltier is activated,

the digit 10 flashes until the condenser switches on again to pre-

vent the Peltier from being destroyed when the condenser is not

running. When the condenser starts up, the flashing starts and the

10 minutes are counted down.

32 Operating Manual V 1.1 – 05/2007

For trimming values above a sec-

tion thickness of 200 μm, the dis-

play flashes to expressly notify

the user of thick sections!

Control Panel Field 2 – Electric Coarse Feed, Sectioning and Trimming Thickness Display

1

Specimen head forwards – pressing briefly

advances the specimen 20μm

Specimen head fast

feed

Fig. 33

Move specimen head backwards slowly – pressing

briefly retracts the specimen 20 μm

Move specimen head backwards

quickly to home position (latched)

2

LED for trim

and section

thickness

display

Illuminates in

yellow when the

specimen is in

retraction.

Toggle TRIM and

SECT (LED

active). Rocking mode – only in manual mode;

in rear area, i.e. approx. 12 - 3 o’clock position of

the handwheel (rock handwheel back and forth

a short distance)

Setting section/trim thickness

Use the - keys on control panel 2.

Range of section thickness: 1 - 100 μm

Values: 1.0μm - 5.0μm in 0.5μm steps

5.0μm - 20.0 μm in 1.0 μm steps

20.0μm - 60.0 μm in 5.0 μm steps

60.0μm -100.0 μm in 10.0 μm steps

7. Instrument Controls

Retraction:off = 0

on = 20 μm

In manual mode.

Range of trim thickness: 1 - 600 μm

(Recommended for research applications)

Values: 1.0 μm - 10.0 μm in 1.0 μm steps

10.0μm - 20.0 μm in 2.0 μm steps

20.0μm - 50.0 μm in 5.0 μm steps

50.0 μm - 100.0 μm in 10.0 μm steps

100.0 μm - 600.0 μm in 50.0 μm steps

Trimming section thickness setting range:

(Recommended for clinical applications)

Values: 10μm, 20μm, 30 μm, 40 μm,

In the "off" setting, there is

no retraction in manual,

automatic or rocking mode.

Press approx. 3 sec.; "on" or

"off" appears (for the

retraction). Toggle using the

"+" or "-" key

For motorized sectioning, the re-

traction value is fixed and cannot

be changed (see page 39 - lower

infobox).

33

Leica CM1950 – Cryostat

7. Instrument Controls

Coarse feed functions

The two-speed electric coarse feed is designed to quickly position the spec-

imen in relation to the knife. The buttons with double arrows move the spec-

imen at 900 μm/s; the buttons with single arrows move it at 300 μm/s.

Retracting the specimen head from the knife

• Pressing once starts the rapid backwards movement to the rear end

position (Home position).

The LED (1) flashes while the specimen head is in motion.

The LED (1) lights up when the rear end position (Hp.) has been reached.

• The retraction can be stopped by pressing one of the coarse drive buttons.

• The rapid backwards movement to the rear end position (Hp.) starts.

The motion continues as long as the button is held.

• A brief press of the button retracts the specimen by 20μm.

Manual sectioning mode

Select ROCK operating mode (LED active) – retraction must be enabled!

• For sectioning, turn the handwheel a short distance (approx. 1/4 turn)

forwards and back (rocking mode) – only possible at rear (handwheel in

approx. 12 - 3 o'clock position).

Every change in rotation direction is electronically detected and automati-

cally translated into a specimen feed or retraction movement.

1

2

slow

fast

slow

fast

Advancing the specimen toward the knife

• Start the slow forwards movement to the knife.

To feed the specimen, press and hold the button.

• Pressing the button briefly results in a feed motion of 20μm.

• Start the fast forwards movement to the knife.

• The LED (2) flashes while the specimen head is in motion.

The LED (2) lights up when the forward end position has been reached.

34 Operating Manual V 1.1 – 05/2007

Sectioning modes

The instrument can be used in manual and motorized modes.

The following settings are available:

•Single stroke (SINGLE) or continuous stroke (CONT.) in automatic mode,

as well as

•ROCK (sectioning using handwheel) in manual mode.

When switching the instrument on, no operating mode is active for

safety reasons.

Hold the button to section

at maximum speed.

Release the button to continue section-

ing at the previously selected speed

(see controller, above).

Control panel 3 – Motorized sectioning (optional)

7. Instrument Controls

Switching the sectioning mode

("CUT MODE") from continuous

stroke ("CONT") to single stroke

("SINGLE") (active)

1. Press the buttons at the same time to start

motorized sectioning.

2. To end the sectioning process, press RUN/STOP,

ENABLE or BRAKE.

- Specimen head stops at bottom (for BRAKE,

automatic electronic braking takes place).

3. For motorized sectioning, does not have to be

unlocked; continue working by pressing both

the RUN/STOP and ENABLE keys.

4. If, during manual work using handwheel, you have

braked using BRAKE also use BRAKE to release!

Press to brake the handwheel electronically

(LED lit) – specimen stops in lower (6 o’clock)

position.

Can be used in any position.

Motor speed controller (0-100%)

The red LED in the E-STOP field indicates

either:

• emergency-stop function active, or

• footswitch dummy (optional footswitch)

connected incorrectly or not at all.

The mechanical handwheel brake is enabled

when the yellow LED is lit in the M-STOP field.

Fig. 34

The handwheel must also be locked

when working on the specimen head.

If emergency stop has been activat-

ed, the cutting mode must be select-

ed again.

Switch from slow speed

range to fast speed range:

while switching on the in-

strument, press and hold

down the Vmax button.

Speed ranges

Slow: 0-50 strokes/min

Fast: 0-85 strokes/min

Vmax: 85-90 strokes/min

35

Leica CM1950 – Cryostat

7. Instrument Controls

Disinfection

Duration - 30min

Duration - 180 min

UVC key - to activate / deac-

tivate the disinfection cycle

and/or to acknowledge in-

terruption of a disinfection

cycle.

To start disinfection the sliding window must be

completely closed.

• Press UVC key once briefly to start the

30 min. mode

• Press UVC key once for a longer time

(approx. 4sec.) to start the 180 min. mode

Specimens and section waste must be thoroughly removed from

the cryochamber first (e. g. using the optional vacuum extractor, or

a paper towel soaked with Cryofect or alcoholic disinfectant).

Before UV disinfection, move the anti-roll guide to the side to

allow complete disinfection.

• Leica Cryofect is not available in all countries.

When the keypad lock is activated (via the key button) the UV lamp

can be shut off only by opening the glass, as the UV keys are

locked.

The cancellation can be acknowledged only if the keypad lock is

disabled. Only then can the UV lamp be switched back on.

Opening the sliding window cancels the disinfection cycle. Press

the UVC key to acknowledge this.

Fig. 35

The germicidal UV lamp is a simple and safe disinfection device

which considerably reduces the risk of infection.

Nevertheless, UV light disinfection cannot replace chemical

disinfection of the cryochamber at regular intervals.

UV light disinfection is effective only on those surfaces that are

directly illuminated.

36 Operating Manual V 1.1 – 05/2007

8. Working with the Instrument

8.1 Preparing Cutting Tools, Specimen Discs and Preparation Aids

The knives are extremely sharp! Handle with care!

Never try to catch a falling knife!

• Place working materials such as the blade box or knives (in the knife case), brush, pincers or

preparation needles and, where applicable, specimen discs into the cryostat chamber.

Fig. 37

• The necessary tools and preparation aids can

be precooled on the (optional) movable shelf,

making them available at all times in a conve-

nient position for the user.

• In addition, specimen discs can be precooled

and stored in the storage system (see page 26,

Fig. 21).

Fig. 36

The instrument must be switched on at least 5 hours before the

planned use.

8.2 Turning the Instrument On

The circuit breaker is used as power switch. The switch must be in the top

position for switching on and in the bottom position for switching off. The

switch must be accessible without obstruction.

• Close the sliding window.

To avoid frost formation always put the cover on the quick freeze

shelf.

Always cover the quick freeze shelf during breaks and overnight.

For installation of knife/blade holder

and installation in the chamber, refer

to optional accessories on page 50).

37

Leica CM1950 Cryostat

8. Working with the Instrument

8.3 Configuring the Parameters

The instrument must be switched on at least 5 hours before the

planned use.

• Turn the lamp on or off.

• This button activates or deactivates the manual defrost of the cooling

chamber, specimen head or freeze shelf. (For more detailed handling

instructions, refer to the chapter on "Working with the Instrument" on

p. 39 of this Operating Manual.)

• Pressing the key button for approx. 5 sec. locks the entire keyboard

(the LEDs in the clock go out).

• Pressing the key button briefly, then the "-" key in the specimen head

control panel field, switches off the specimen head.

• Pressing the key button briefly, then the "+" key in the specimen head

control panel field, switches the specimen head back on.

Programming the temperature of the cryochamber

• The temperature of the cryochamber is set and indicated on the control

panel marked with the cryostat symbol.

The actual temperature is the standard indication.

Briefly pressing the "+" or "-" button displays the target temperature.

Set the desired value via the "+" / "-" buttons. Pushing the "+" or "-" button

for more than 1 sec. increases or decreases the chamber temperature

continuously.

The actual value will be indicated 5 seconds after finishing the program-

ming.

Refer to page 52 for a table with guide values. The temperature

values given there are based on long-term experience, but are

intended solely as guide values, as any tissue may require particu-

lar adjustments.

38 Operating Manual V 1.1 – 05/2007

Setting the time

• The clock time is set with the +/- buttons in the control panel marked

with the clock symbol.

To do so, set the current time using the "+" or "-" button below the small

clock symbol.

Pushing the "+" or "-" button for more than 1 sec. increases or decreases

the time continuously (auto-repeat function).

8. Working with the Instrument

Programming the specimen temperature

• Select the desired temperature of the specimen.

• The specimen temperature is set and indicated on the control panel

marked with the specimen head symbol.

The actual temperature is the standard indication.

Briefly pressing the "+" or "-" button displays the target temperature.

Set the desired value via the "+" / "-" buttons. Pushing the "+" or "-" button

for more than 1 sec. increases or decreases the specimen temperature

continuously.

The actual value will be indicated 5 seconds after finishing the program-

ming.

Specimen temperature - "Max-Cool" function

• The snowflake button for the Max-Cool function is in the panel with the

specimen head symbol.

Pressing the button sets the lowest possible specimen head tem-

perature (-50°C) as the target temperature. The instrument adjusts the

maximum low temperature of the specimen head, i.e. -50°C.

• Push the snowflake button again to stop the "Max-Cool" function.

The temperature adjusts to the value programmed prior to activating the

"Max-Cool"-function.

• Alternate flashing of "LL" and the actual temperature indicates activa-

tion of the Max-Cool function.

Caution:

The specimen head and Peltier do not switch on until the chamber

temperature reaches -5 °C, in order to prevent icing.

39

Leica CM1950 Cryostat

8. Working with the Instrument

Programming the defrost cycle

• Set the beginning of the automatic defrost cycle.

The automatic defrost cycle takes place once within 24 hours.

It is set with the "+"/"-" buttons on the right of the panel with the clock

symbol. The two buttons are marked by a melting snowflake .

• Briefly press the "+" or "-" button for indication of the beginning of the

defrost cycle which has actually been set. At the same time, the LEDs

between the indication of hours and minutes are flashing.

• To change the beginning of the defrost cycle in steps of 15 minutes, push

the "+" or "-" key. When the "+" or "-" button is pushed for more than 1 s,

the defrost time value increases or decreases continuously.

Before starting the defrost cycle remove all samples from the

cryochamber!

• When the automatic defrost cycle begins, the specimen head tempera-

ture adjusts to a temperature between - 10°C and - 5°C (reduction of ice

formation). The specimen head cooling turns off. This is confirmed by the

flashing of the decimal points on the panel for the specimen cooling. The

specimen cooling (controlled to set value) automatically turns back on

after 4 hours, once the chamber temperature varies by less than 5 K from

the target temperature.

• If you want to turn the specimen cooling back on manually before the

automatic activation sets in, push the "+" or "-" button on the panel for the

specimen cooling and then the key button.

• The temperature of the specimen cooling first raises to +10°C and then

adjusts to the programmed specimen temperature.

Manual defrost for the freeze shelf (including Peltier element)

• Press the key for manual defrost , then the key.

The manual defrost takes 12 min.

• Push the button and then the key again to stop the manual defrost.

After defrosting the freeze shelf, carefully wipe it out, as otherwise

a lot of water collects in the channel. Ice does not melt during

normal defrosting.

40 Operating Manual V 1.1 – 05/2007

8. Working with the Instrument

Manual defrosting of the cryochamber

• Push the manual defrost button (with the melting snowflake) on the left

over the key button to activate the defrost cycle of the cryochamber on

demand.

• Activation is confirmed by an audible signal.

•

Then, push the "+" or "-" button on the panel for the cryochamber temperature.

• The manual defrost cycle (12 min.) is activated.

• There is a flashing indication of the temperature of the cryochamber

during the whole defrost cycle.

• If necessary, push the manual defrost button again to deactivate the

manual defrost cycle.

• When the manual defrost begins, the specimen head adjusts to a tem-

perature between -10°C and -5°C (reduction of ice formation). The

specimen head cooling turns off. This is confirmed by the flashing of the

decimal points on the panel for the specimen cooling.

• Ten seconds after the manual defrost cycle has been completed, the

specimen cooling turns back on.

Before starting the defrost cycle, remove all specimens from the

cryochamber!

Manual defrosting of the specimen cooling

• Push the manual defrost button (with the melting snowflake) on the left

over the key button to activate the defrost cycle of the specimen head.

• Activation is confirmed by an audible signal.

• Then, push the "+" or "-" button on the panel for the specimen temperature.

• There is a flashing indication of the specimen temperature during the

defrost cycle.

• For 15 minutes, the specimen head is adjusted to a temperature of 45°C.

• Subsequently, the instrument adjusts to the specimen temperature which

has been programmed prior to the manual defrost cycle.

• If necessary, push the manual defrost button again to deactivate the

manual defrost cycle.

Press the key button, then the "+" button = specimen head on

Press the key button, then the "-" button = specimen head off

41

Leica CM1950 Cryostat

8. Working with the Instrument

Entering the trimming thickness

To switch from a trimming section thickness for research applica-

tions (1-600 μm) to a thickness for clinical applications (10, 20,

30 or 40 μm), press and hold down the TRIM/SECT key while

switching on the instrument.

• Press the TRIM/SECT button. TRIM mode is active if the LED at the top

right is illuminated.

• Set the desired trimming thickness using the "+" or "-" button in control

panel field 2. (Refer to page 32 for adjustable sequence of steps)

Entering the section thickness

• Press the TRIM/SECT button. SECT mode is active if the LED at the bot-

tom right is illuminated.

• Set the desired section thickness using the "+" or "-" button in control

panel field 2. (Refer to page 32 for adjustable sequence of steps)

Switching the retraction on or off in manual sectioning mode

• Press the TRIM/SECT button for approx. 3 sec. The LED in control panel

field 2 displays on or oFF .

• You can switch by pressing the "+" or "-" button.

• "Retraction on" means a specimen retraction of 20 μm in manual mode.

During motorized sectioning, the retraction value is speed-

dependent and cannot be changed by the user.

42 Operating Manual V 1.1 – 05/2007

8. Working with the Instrument

8.4 Working with the Precooled Cryostat

8.4.1 Preparatory Work

• Lock the handwheel in the top (12 o’clock) position.

• Cut the specimen to size outside of the cryostat.

• Select the precooled specimen disc, cover it with freezing compound,

then attach and orient the specimen.

• Attach the specimen disc and specimen to the Peltier position on the

freeze shelf. Activate the Peltier element and wait until the specimen is

completely frozen.

Specimens that have been frozen on the Peltier element are often

too cold and split apart during sectioning. Allow time for the speci-

mens to become acclimated.

• Insert the specimen disc into the specimen head.

• Fold the anti-roll guide away and to the side. Insert a precooled knife or

blade.

Before using them for the first time, degrease new knives using

acetone or alcohol.

• Approach the specimen with the knife or blade holder:

• To do so, open the clamping lever of the base, approach the

specimen and close the lever again.

• Open the lever of the orientation. Orient the specimen (move it into

a favorable position relative to the knife/blade) and close the lever again.

• Approach the knife or blade holder using the coarse feed buttons and

gentle movements of the handwheel.

If the sections are cracked, the specimen head temperature is too

cold. Set a warmer temperature.

If the sections smear, the specimen head temperature is too warm.

Set a colder temperature.

43

Leica CM1950 – Cryostat

8. Working with the Instrument

8.4.2 Trimming with Extraction – Anti-roll Guide Installed

• Remove the silicone stopper from the filter cover (and keep it in a safe

place).

• Insert the extraction hose with the black adapter.

• Fold the anti-roll guide to the side and fasten the suction nozzle to the

pressure plate – see mark Fig. 39a - (using 4 magnets on the rear side of

the nozzle).

• Fold the anti-roll guide back into position.

E

Ensure that the hose with the nozzle is

not installed against its "natural" cur-

vature on the pressure plate of the

blade holder.

The tension acting on the hose can be minimized

by turning the red ring (Fig. 39, top right) clock-

wise so that the suction nozzle presses against

the pressure plate (I, Fig. 39).

A

D

I

B

C

Fig. 40

K

• Check that the anti-roll guide is parallel and correctly adjusted.

Readjust if necessary (see page 58).

• Activate trimming mode.

• Select the trimming thickness.

• Switch on the VAC and select a low extraction level (between 1 and 2).

•

Start trimming by manually moving the handwheel or press the

RUN/STOP and ENABLE keys simultaneously to start motorized sectioning.

Fig. 38

Fig. 39a Fig. 39b Fig. 39c

44 Operating Manual V 1.1 – 05/2007

8. Working with the Instrument

For motorized sectioning, always begin at low speed for safety

reasons.

• Optimize the vacuum settings if necessary.

• Press the "VAC" button to enable the vacuum extractor. The LED in the

"VAC" button is lit while the extractor is on. Press the button again to

disable it.

• Use the knob to adjust the intensity of the vacuum.

A Optimal area for trimming and sectioning

• Trimming: Handwheel position 12 - 6 o’clock, valve open

Handwheel position 6 - 12 o’clock, valve closed

• Cutting: Handwheel position 12 - 3 o’clock, valve open all the way

Handwheel position 3 - 6 o’clock, valve half open

Handwheel position 6 - 12 o’clock, valve closed

B Optimal area for extraction from the chamber

• To clean the chamber, turn the knob to the red range.

If the handwheel is not moved for approx. 5 sec, the valves close and the fan remains on.

If the handwheel is moved for approx. 1 min, the valves close, the fan shuts off (the LED in the

VAC switch goes off to prevent icing).

To continue working, you now have to reactivate the VAC.

2. Trimming with Extraction – Brush Technique, finger rest installed

• Remove the silicone stopper (E) from the filter cover (and keep it in a safe

place).

• Insert the extraction hose with the black adapter.

• Fasten the suction nozzle to the pressure plate (using 4 magnets on the

rear side of the nozzle) as far as possible towards the blade.

Ensure that the hose with the nozzle is not installed against its "natural" curvature on the

pressure plate of the blade holder.

The tension acting on the hose can be minimized by turning the red ring

(Fig. 28 on page 28) clockwise so that the suction nozzle presses against the

pressure plate.

• Check that the suction nozzle is seated optimally (by gently turning the

handwheel)

A

B

E

45

Leica CM1950 Cryostat

8. Working with the Instrument

• Activate trimming mode.

• Select the trimming thickness.

• Switch on the VAC and select a low extraction level (between 1 and 2).

• Start trimming by manually moving the handwheel or press the

RUN/STOP and ENABLE buttons simultaneously to start motorized

sectioning.

• Use a brush to apply the section to a precooled specimen slide, then

warm it from below using your finger.

• Move the knife guard forward after removing the section.

• Remove the knife or disposable blade (using the blade ejector!).

• Insert the knife into the knife case.

8.4.3 Cutting with Extraction – Anti-roll Guide installed

• Shut off VAC (LED in VAC button goes out).

• Switch from trimming to sectioning mode (important for section stretch-

ing, as the valves work differently than in trimming mode.)

• Set the desired section thickness.

• Switch on the VAC and begin at level 1. If the section does not stretch

correctly, turn the VAC knob higher in small increments.

• Once the desired section is on the pressure plate, switch off the VAC!

Carefully fold the anti-roll guide to the side and remove the section from

the side.

After removing the section, wipe off moisture/condensate from the

pressure plate – otherwise, the next sections will become

jammed.

Cutting with extraction without anti-roll guide (brush technique) is

not possible, as the position of the pressure plate means that no

suitable air flow is obtained.

46 Operating Manual V 1.1 – 05/2007

A few rules:

• Always begin at a low extraction level, then slowly increase it.

• Do not use high extraction levels unless absolutely necessary.

• Different specimen sizes require different extraction levels.

• The faster the trimming or sectioning speed, the lower the extraction

level should be.

• The larger and/or thicker the specimen to be trimmed, the lower the

extraction value.

• When section specimens with a diameter of 0.5 cm, the anti-roll guide

stretches the section adequately. For larger specimens, we recommend

using the vacuum function.

8. Working with the Instrument

Trimming, 1 x 1cm specimen

Trimming thickness Speed Extract. value

10μm 40% 1.5

10μm 60% 1.5

10μm 80% 1

10μm 100% 1

20μm 40% 2

20μm 60% 2

20μm 80% 1.5

20μm 100% 1

30μm 40% 2.5

30μm 60% 2.5

30μm 80% 2

30μm 100% 1.5

40μm 40% 3

40μm 60% 3

40μm 80% 2.5

40μm 100% 2

Trimming, 1 x 1cm specimen

Trimming thickness Speed Extract. value

1 μm - 5 μm 40% 2.5

60% 2

80% 1.5

100% 1.5

10μm 40% 2.5

60% 2

80% 2

100% 1.5

The values listed here are possible

settings that depend on the tissue

type, specimen size, section thick-

ness and section speed, and are in-

tended for orientation purposes only.

These settings can also vary when using

different anti-roll plate!

47

Leica CM1950 Cryostat

8. Working with the Instrument

After trimming or sectioning:

Specimen:

• Unclamp and thaw.

• Immerse in fixative for further processing.

Cleaning

• Use the brush to sweep up the section waste (section waste tray) and

remove it from the cryostat (follow applicable laboratory regulations for

disposal).

Or:

• Clean the cryostat chamber using the chamber suction nozzle:

• To do so, turn the (flat) suction nozzle of the extraction hose by

holding the hose on the white adapter and removing it with a quick

twist. "Park" the flat suction nozzle in a designated place in the

chamber, e.g. on the right inside wall of the cryogenic chamber.

• Remove the chamber suction nozzle from the plastic clip and

attach it firmly to the white adapter.

• Check the remaining capacity of the filter (in the interior of the chamber)

and change the filter if necessary (see page 28).

• Check the bacterial filter (in the front of the unit, see page 28), change it

at least every 3 months.

• Move the anti-roll guide to the side.

• Spray the cryostat chamber using Cryofect.

• Activate the UVC disinfection.

48 Operating Manual V1.1 – 05/2007

Problem Causes Remedies

Frost on chamber walls and

microtome

Sections smear

Sections splinter

Sections not properly

flattened

Sections not properly

flattened despite correct

temperature and correctly

aligned anti-roll plate

Sections curl on anti-roll

plate

Scraping noise during

sectioning and specimen

return movement

Cryostat is exposed to air currents

(open windows and doors, air

conditioning).

Frost built-up by breathing into the

cryochamber.

Specimen not cold enough.

Anti-roll plate not yet cold enough,

thus warming the section.

Specimen too cold.

Static electricity/air currents.

Specimen not cold enough.

Large area specimen.

Anti-roll plate poorly positioned.

Anti-roll plate poorly aligned with knife

edge.

Incorrect clearance angle.

Blunt knife.

Dirt on knife and/or anti-roll plate.

Edge of anti-roll plate damaged.

Blunt knife.

Anti-roll plate does not protrude far

enough beyond the knife edge.

Anti-roll plate protrudes too far beyond

the knife edge and is scraping against

the specimen.

Change place of installation for the

cryostat.

Wear mouth protection.

Select lower temperature.

Wait until knife and/or anti-roll

plate have reached chamber

temperature.

Select higher temperature.

Remove cause.

Select lower temperature.

Trim the specimen parallel,

increase section thickness.

Reposition anti-roll plate.

Align correctly.

Set correct angle.

Use different part of the knife.

Clean with dry cloth or brush.

Replace anti-roll plate.

Use different part of the knife.

Readjust correctly.

Readjust correctly.

9. Troubleshooting

9.1 Problems During Work

Leica CM1950 Cryostat 49

Problem Causes Remedies

Ridged sections

Chatter during sectioning

Condensation on anti-roll

plate and knife during

cleaning

Anti-roll plate damaged

after adjustment

Thick-thin sections

Knife damaged.

Edge of anti-roll plate damaged.

Specimen insufficiently frozen onto

the specimen disc.

Specimen disc not clamped tightly.

Knife not clamped tightly enough.

Specimen has been sectioned too

thickly and has detached from disc.

Very hard, inhomogenous specimen.

Blunt knife.

Knife profile inappropriate for

specimen cut.

Incorrect clearance angle.

Brush, forceps and/or cloth too warm.

Anti-roll plate too high above the knife

edge. The adjustment was carried out

in the direction of the knife.

Temperature incorrect for the

tissue cut.

Knife profile inappropriate for the

specimen cut.

Ice buildup on the knife back.

Handwheel speed not uniform.

Knife not clamped tightly enough.

Specimen disc not clamped tightly

enough.

Cryocompound applied to cold

specimen disc; specimen detached

from disc after freezing.

Use different part of the knife.

Replace anti-roll plate.

Refreeze specimen onto the disc.

Check disc clamping.

Check knife clamping.

Refreeze specimen onto the disc.

Increase section thickness; reduce

specimen surface area if ecessary.

Use different part of the knife.

Use knife with different profile.

Set correct angle.

Store all tools on storage shelf in

the cryochamber.

Replace anti-roll plate.

Be more careful next time!

Select correct temperature.

Use knife with different profile

(c or d).

Remove ice.

Adapt speed.

Check knife clamping.

Check disc clamping.

Apply cryocompound to warm disc,

mount specimen and freeze.

9. Troubleshooting

50 Operating Manual V1.1 – 05/2007

Problem Causes Remedies

Blunt knife.

Inappropriate section thickness.

Incorrect clearance angle.

Microtome not dried thoroughly

enough.

Dried specimen.

Anti-roll plate is too warm or

incorrectly positioned.

Fat on the corner or edge of anti-roll

plate.

Anti-roll plate not correctly fixed.

Rust on the knife.

Anti-roll plate too warm.

Temperature too low for the tissue cut.

Blunt part, dirt, dust, frost or rust on

the knife.

Top edge of the anti-roll plate

damaged.

Hard particles in the tissue.

Dirt on knife back.

Power plug not properly connected.

Defective fuses, or circuit breaker has

triggered.

Moisture on the underside cause the

specimen to freeze to the freezing

shelf or specimen head.

Use different part of the knife.

Select correct section thickness.

Set correct angle.

Dry microtome.

Prepare new specimen.

Cool down anti-roll plate, or

reposition correctly.

Remove fat from anti-roll plate.

Fix correctly.

Remove rust.

Cool down anti-roll plate.

Increase temperature and wait.

Remove cause.

Replace anti-roll plate.

- - -

Clean.

Check if properly connected.

Replace fuses, or switch circuit

breaker back on. If not possible,

call technical service.

Apply concentrated alcohol to the

contact point.

Tissue sticks to the anti-roll

plate

Flattened sections curl up

when anti-roll plate is

folded up

Sections tear

Cryostat non-operational

Specimen disc cannot be

removed

9. Troubleshooting

Leica CM1950 Cryostat 51

Problem Causes Remedies

No or insufficient

refrigeration of the

cryochamber

Sliding window collects

condensation

No or insufficient

refrigeration of the

specimen

Lamp does not work

Both disinfection LEDs flash

alternately

Cooling system or electronic drive

defective.

Air humidity and room temperature

too high.

Cooling system or electronic drive

defective.

Lamp defective.

Switch defective.

UV radiation provided by UV tube no

longer sufficient.

Image of an open-ended wrench

appears due to fault to be rectified.

Call technical service.

Comply with the requirements

for the installation site.

Call technical service.

Check lamp and replace it, if

necessary.

Call technical service.

Replace UV tube following the

manufacturer’s instructions.

Contact technical service and

follow the instructions given.

9. Troubleshooting

52 Operating Manual V 1.1 – 05/2007

10. Temperature Selection Chart (in minus °C)

Tissue type Chamber temperature Specimen head temperature

Spleen -15°C to -20°C -11°C

Liver -10 °C -20 °C

-15°C off to -15°C

Intestine -10°C -20°C

-15°C A*: off to -20°C

E*: -20°C

Heart -10°C A: -20°C

E: -20 °C to -30 °C

-15°C off to -20°C

Ovaries -10°C E: -20 °C

-15°C off to -15°C

Fallopian tubes -10° E: -20°C

-15°C off to -15°C

Kidney -10 °C -20 °C

-15°C A: off to -15°C

-20 °C -20 °C

Muscle -18°C to -20°C -15°C

Skin with fat -19°C -32°C to -40 °C

Hard fat -19°C -21°C to -25 °C

Stomach -10 °C -20 °C

-15°C off to -15°C

Brain -15°C -10°C, *E

The temperature values given above are based on long-term experience, however, these are only approximate

values, as any tissue may require particular adjustments.

*A = blocked, *E = complete

53

Leica CM1950 – Cryostat

11.1 Ordering Information

Specimen disc ø 20mm assembly .................................................... 0477 43739

Specimen disc ø 30mm assembly .................................................... 0477 40044

Specimen disc ø 40mm assembly .................................................... 0477 40045

Specimen disc ø 55mm assembly .................................................... 0477 40046

Specimen disc 80x 50 mm, assembly ............................................... 0477 43714

O-ring blue (10 pieces), 20 mm.......................................................... 0477 43247

O-ring red (10 pieces), 20 mm ........................................................... 0477 43248

O-ring blue (10 pieces), 30 mm.......................................................... 0477 43247

O-ring red (10 pieces), 30 mm ........................................................... 0477 43248

O-ring blue (10 pieces), 40 mm.......................................................... 0477 43249

O-ring red (10 pieces), 40 mm ........................................................... 0477 43250

O-ring blue (10 pieces), 55 mm.......................................................... 0477 43251

O-ring red (10 pieces), 55 mm ........................................................... 0477 43252

Blade holder base complete.............................................................. 0477 40351

Blade holderextension CE-BB, complete ........................................ 0477 43005

Insert for low-profile blades for blade holder CE, complete ........ 0477 42488

Finger rest, complete ........................................................................... 047740387

Anti-roll plate 70 mm - 50μm spacer ................................................ 0477 42491

Anti-roll plate 70 mm -100μm spacer................................................ 0477 42492

Anti-roll plate 70 mm -150μm spacer................................................ 0477 42493

Glass insert 70mm, polished.............................................................. 0477 42497

Knife holder base assembly............................................................... 0477 42359

Knife holder CN assembly .................................................................. 0477 42358

Knife support CN short ....................................................................... 0477 42380

Knife support CN ................................................................................. 0477 42370

Knife holder attachment CNZ, assembly ......................................... 0477 42363

Anti-roll plate assembly glass 50mm ................................................ 0419 33981

Heat and cold extractor, assembly................................................... 0477 41039

Cold extractor, assembly.................................................................... 0477 43737

Heat extractor, assembly ................................................................... 0477 43126

11. Optional Accessories

54 Operating Manual V 1.1 – 05/2007

Section waste tray .............................................................................. 0477 40062

Brush shelf ........................................................................................... 0477 43036

Storage system, assembly ................................................................. 0477 42618

Shelf, movable ..................................................................................... 0477 43037

Retaining device .................................................................................. 0477 40080

Cover freezing shelf ............................................................................ 0477 43763

Specimen disc 37x 37mm ................................................................... 0477 42603

Specimen disc 28x 28mm ................................................................... 0477 42604

Bar, large .............................................................................................. 0477 42600

Bar, medium size ................................................................................. 0477 42601

Bar, small .............................................................................................. 0477 42602

Heat extractor, Dr. Peters, assembly ............................................... 0477 41338

Freezing griddle/block elevator......................................................... 0201 39119

Dispensing slides 8 pieces................................................................. 0201 39127

Foot rest assembly .............................................................................. 0477 42832

Accessory kit extraction .................................................................... 0477 43300

Bactericidal filter 350/5865................................................................. 0477 40296

Hose set, silicon 5 pieces................................................................... 0477 44469

Filter assembly 25 pieces ................................................................... 0477 44307

Cryofect, VPE Leica Cryofect 4x 350ml,

low-temp. disinfectant to -20°C......................................................... 0387 42801

Safety gloves size M ........................................................................... 0340 29011

Safety gloves size S ............................................................................ 0340 40859

Footswitch, dummy plug CM 3050 .................................................... 0443 30420

Laboratory chair on sliders (8030442) .............................................. 0710 34911

Footswitch, assembly ......................................................................... 0505 33888

Easy Dip staining container white ................................................... 0712 40150

Easy Dip staining container pink....................................................... 0712 40151

Easy Dip staining container green.................................................... 0712 40152

Easy Dip staining container yellow .................................................. 0712 40153

Easy Dip staining container blue ...................................................... 0712 40154

Easy Dip staining rack gray ............................................................... 0712 40161

11. Optional Accessories

55

Leica CM1950 – Cryostat

Assembling the anti-roll guide system

(for the blade holder CE)

1. Insert the glare-minimizing glass insert into the

interchangeable frame and tighten it evenly

using the knurled screw (10).

2. Insert the shaft (11) of the metal frame for

exchangeable glass inserts from above into

the bore of the swinging arm (12) in such a way

that the pin rests in the notch.

3. Push the white plastic plate (14) from below

onto the shaft (11).

4. Screw the knurled nut (13) from below onto

the shaft (11).

The glass of the anti-roll guide can be

used from all 4 sides when replace-

ment is necessary (the glass stage

plate can be reordered).

13

14

11

6

10

10

• With blade ejector (1)

• With knife guard (2)

• Integrated lateral shift and stable base

• Clearance angle adjustment (5) with 3 mm

Allen key (see detail image 5, to left at knife

holder) - recommended angle from 2° to 5°.

• With anti-roll guide (4)

• Lever (6) for the lateral shift

• Lever (7) for clamping the blade

• Lever (8) for clamping the base (9) to the

dovetail guide in the chamber

• Pressure plate (3) for section extraction

• When using low-profile blades, the ruler

(7, Fig. ) must be inserted.

Blade holder CE with anti-roll guide for low-profile (NB) and high-profile (BB)

1

4

5

2

3

11. Optional Accessories

Fig. 41

Fig. 41a

Fig. 42

4

6

7

8

9

12

56 Operating Manual V 1.1 – 05/2007

The knife guard is folded

away in the detail view.

Knife guard folded away

Fig. 43

Four possible

settings

Blade rest (ruler) for low-profile

blades

1

2

1

2

6

Fig. 44

11. Optional Accessories

Red elements on the blade or knife

holders, such as the knife guard and

ejector, are protective devices that

may not be removed.

3

4

7

The anti-roll guide and

pressure plate must be

parallel to each other.

6

• Blade ejector (1) and knife guard (2)

• Integrated lateral shift and stable base

• Clearance angle adjustment using size 3 Allen

key (recommended angle between 2° and 5°)

• With finger rest (6) for brush specimen

• Clamping lever (8) for lateral shift must point

downward to permit unhindered shifting of

finger rest.

• when using BB knives, remove the knife

support (7).

Conversion of blade holder with anti-roll guide

to blade holder with finger rest

• Screw off the anti-roll guide.

• Unscrew left Allen screw using 2.5 mm Allen

key and remove base of anti-roll guide.

• Attach the finger rest (6) from the left, tighten

the hexagon-head screw using the No. 2.5

key – be careful of the blade ejector!

If you are working with the brush

technique, the knife guard must be

folded upwards.

Blade holder CE with finger rest (for NB + BB)

7

8

5

57

Leica CM1950 – Cryostat

Anti-roll guide system functions as a

knife guard here!

1. Fold the anti-roll guide system (4) to the left –

while doing so, hold the lever (11) (not the

adjusting screw of the anti-roll guide) so that

the height of the anti-roll guide remains

unchanged.

2. Open the clamping lever (10) by turning it coun-

terclockwise.

3. Carefully insert the blade (9) from above or

from the side between the pressure plate and

the blade rest. Ensure that the blade is insert-

ed so that it is centered.

4. Rotate lever (10) clockwise to clamp.

5. Fold the anti-roll guide system (4) back to the

side using the lever (11).

Lateral shift for Blade Holder CE

If the sectioning results are not satisfactory, the

knife holder can be shifted sideways in order to

use another part of the blade.

To do so, follow these steps:

1. Release the clamping lever (8) by folding it

back and move the knife holder back until the

desired position is reached (3-point click stop

enables you to accurately find a new cutting

position).

2. Fold the clamping lever (8) forwards for

clamping.

Inserting the blade into knife holder CE

Fig. 45

11. Optional Accessories

8

9

7

10

11

4

Fig. 46

8

58 Operating Manual V 1.1 – 05/2007

11. Optional Accessories

Adjusting the Knife Holder with Anti-roll Guide

You can adjust the height of the anti-roll guide

system using the knurled nut (1):

• If you turn the nut counterclockwise, the anti-

roll guide system moves toward the blade.

• If you turn the nut clockwise, the anti-roll guide

system moves away from the blade.

If the anti-roll guide system is in the wrong posi-

tion relative to the cutter, the following problems

will result:

Fig. I: The section rolls over the glass insert of the anti-roll guide system.

Error: Glass insert not high enough.

Remedy: Turn the knurled nut counterclockwise until the section is pushed between the

blade and anti-roll guide as shown in Fig. III.

Fig. II: Section tears and block hits the glass insert after sectioning. The section rolls

over the glass insert of the anti-roll guide system.

Error: Anti-roll guide system is set too high.

Remedy: Turn the knurled nut clockwise until the section is pushed between the blade

and anti-roll guide as shown in Fig. III.

Generally, we recommend pre-adjusting the anti-roll guide system at a high sec-

tion thickness (e.g. 10μm). Start from there and work your way down to the desired

section thickness in small increments, readjusting the anti-roll guide system at

each increment using the knurled nut.

Fig. 47

I

II

III

1

Blade holder CE with glass anti-roll guide

Fig. 48,

Knife holder CN with glass anti-roll guide

59

Leica CM1950 – Cryostat

Cleaning the knife holder CN

Daily cleaning

For daily cleaning, it is enough to fold the

anti-roll guide system forwards and remove

the section waste from the knife holder using

a dry brush. Please use a cold brush, as

otherwise the section waste will thaw and

stick to the knife holder.

It is not necessary to oil the parts, such

as the T-piece on the microtome base

plate, clamping lever etc.

Fig. 49

Fig. 50

Cleaning the knife holder CE

Daily cleaning

1. Fold the anti-roll guide system (33) to the left

while holding it down by the lever (34).

2. Unscrew the bolts (35) of the pressure plate.

3. Afterwards, the pressure plate (36) can be re-

moved for cleaning (with alcohol or acetone).

For disinfection, commercially avail-

able, mild cleaners and disinfectants

can be used – we recommend Leica Cry-

ofect.

11. Optional Accessories

Disinfection (e.g. with Leica Cryofect)

Spray contaminated surfaces with a uniform coat of concentrate or wipe

them down with a rag soaked in it, allow it to make contact for 15 minutes,

then wipe it off.

33

34

35

35

36

60 Operating Manual V 1.1 – 05/2007

1

Knife holder CN with anti-roll guide – Moving

the clamping chucks

The large specimen disc (80x 50 mm) should pref-

erably be used with knife holder CN and the 16cm

C-profile steel knife.

The clamping jaws are factory-installed in the

knife holder with a clearance of 64mm. If neces-

sary, both clamping chucks can be offset with a

clearance of 84mm.

Perform the following steps:

• Use a 3 mm Allen key to loosen the screw over

the clearance angle adjustment (1) and

remove the segment arch (2) from the knife

holder base.

• Use a 3 mm Allen key to loosen the screws (4)

on the underside of the segment arch.

• Carefully pull the clamping jaw (5) on the right

upward and insert it in the neighboring hole

(6). Tighten the screws from the underside of

the segment arch. Repeat on the left side.

• Insert the long knife support (3) on the side

over the knurled screw (3a) so that the recess

faces the user – turn the height adjustment

knurled screws until the lower stop is reached.

• The knife can now be inserted from the side

and its height adjusted via the knurled screws

(3a).

2

3a

44

5

6

3

Fig. 51

3a

not

visible

11. Optional Accessories

Never work with only one clamping jaw, as this does not ensure the stability required for the

sectioning process. Also, a long knife will not be sufficiently protected by the knife guard in this

instance.

The specimen disc 50x80mm is suitable

only for section thicknesses up to approx.

5μm (due to the large specimen size).

61

Leica CM1950 – Cryostat

Knife holder CNZ with anti-roll guide

• Pressure plate permits the full length of the

knife to be utilized.

• Use hard metal and steel knives here.

Fig. 52

11. Optional Accessories

The height of resharpened knives must be adjusted using knurled

screws (3) (approx. 1 mm under the edge of the clamping jaw).

Ensure that the knife is adjusted in parallel vertically.

Knife guard/lateral movement for knife

holder CN

The knife guard (18) is fixed and integrated into

the clamping jaws. The knife guard has handles

(19) that allow it to be moved. The knife guard is

adequate for knives up to 22 cm in length. Always

cover exposed parts of the knife blade after

sectioning.

The anti-roll guide system can be moved side-

ways (only for the 84 mm variant). To better find

the mid position, a groove (17) is provided in the

shaft (16).

19 19

18

16

17

Fig. 53

62 Operating Manual V 1.1 – 05/2007

Fig. 56

Bacterial filter

• Bactericidal filter 350/5865, pack of 1 unit. Rec-

ommendation: replace bactericidal filter every

three months.

(note date on filter when installing it)

Anti-roll guide system

Anti-roll plate (with glass stage plate)

Available with various spacers:

• 70mm - 50μm, for section thickness: < 20μm

• 70mm -100μm, for section thickness: 20 μm -

100 μm

• 70mm -150 μm, for section thickness: > 100 μm

The 50 μm and 100 μm anti-roll plates

are included in the standard scope of

delivery of the blade holder CE.

70mm

11. Optional accessories

Fig. 55

Fig. 54

Straightedge (blade rest)

Insert for low-profile blades for blade holder CE

(0477 43005) - replacement

The blade rest is also included in the

standard scope of delivery of the

blade holder CE.

Filter bags and bacteria filter must be

disposed of according to applicable

laboratory regulations for infectious

material. Filters must be replaced, not

cleaned.

When using low-profile blades, the blade rest (7)

must be inserted into the blade holder first, then

the low-profile blade (see page 50, Fig. 40).

63

Leica CM1950 – Cryostat

Fig. 57

Fig. 58

Replacement filters for extraction system

(pack of 25)

• Change when vacuum power is impaired.

• When using the cryostat frequently, check the

filter bag daily and replace as required.

Vacuum extraction system

A- Hose

B- Hose adapter, black (for the filter

bag in the instrument)

C- Hose adapter, white (for suction nozzle D

or extraction nozzle F)

D- Suction nozzle – with 4 magnets (E) on

knife holder

G- Plastic clip (for parking the suction

nozzle)

A

B

C

D

E

11. Optional Accessories

F

G

Fig. 59

Storage system, complete ("hidden")

• for installation in rear area of cryostat - for cold

storage of specimen discs and sectioning ac-

cessories

(For assembly, refer to page 26)

64 Operating Manual V 1.1 – 05/2007

Fig. 60

AHeat extractor with parking station, stationary

• for installation in the cryostat - consisting of:

support device, heat extractor and parking sta-

tion

BHeat and cold extractor

• suitable for two different applications: extracts

cold when stored in warm environment; ex-

tracts heat, when stored in cold environment.

A

B

C

• Specimen discs in various dimensions

Under-

side

Fig. 62

• The specimen discs of the Dr. Peters cooling

system can be conveniently removed from the

shelf using the underside of the heat extrac-

tor (removal aid D). Slide the underside over

the specimen disc in the direction of the ar-

row so that the disc remains in the slot and

can be removed from the rack.

11. Optional Accessories

Dr. Peters

specimen discs

D

Fig. 61

50x80 mm ø 55 ø 40 ø 30 ø 20 mm

O-rings in various colors

• for plate Ø 20mm (red or blue), 10x each

• for plate Ø 30mm (red or blue), 10x each

• for plate Ø 40mm (red or blue), 10x each

• for plate Ø 55mm (red or blue), 10x each

for color-marking of pecimen disc

37x 37 mm

28 x 28 mm

Shelves

The specimen disc 50x80mm is suitable

only for section thicknesses up to approx.

5μm (due to the large specimen size).

Embedding well bar, large, with 3 wells, LxWxH: 30 x 30 x 7 mm

Embedding well bar, medium, with 4 wells, LxWxH: 24 x 24 x 6 mm

Embedding well bar, small,with 4 wells, LxWxH: 18 x 18 x 6 mm

65

Leica CM1950 – Cryostat

Footswitch,

for use with motorized instruments only

Fig. 63

Fig. 66

Fig. 65

11. Optional Accessories

Brush shelf,

for use with blade holder CE

Fig. 64

Adjustable support,

individually height-adjustable foot rest with 5 set-

tings.

Storage system, movable

for installation in front area of cryostat - for cold

storage of preparation tools

66 Operating Manual V 1.1 – 05/2007

12. Maintenance and Cleaning

1

Fig. 67

12.1 General Maintenance

The microtome is virtually maintenance-free. To ensure a smooth operation

of the instrument over several years we recommend the following:

• At least once a year, have the instrument inspected by a qualified service

engineer authorized by Leica.

• Enter into a service contract at the end of the warranty period. For further

information, please contact your local Leica service center.

• Clean the instrument daily.

• Every day, remove frozen section waste from the cryostat using a cold

brush or use the (optional) extraction device.

• Remove the section waste tray for emptying.

• Remove the storage shelves and the brush shelf for cleaning.

• Remove the closed sliding window from the front by gently lifting it

(see p. 63, "Replacing the Fluorescent Lamp").

Do not use organic solvents or any other aggressive substances for

cleaning and disinfecting!

Use only disinfectants listed in this manual such as Leica Cryofect

(alcohol or other common alcohol-based disinfectants).

• Drain the cleaning liquid through the hose after the prescribed reagent

time is over and collect it in the waste container (1).

Emptying the condensate bottle

Check the fill level of the condensate bottle (1) visible in the front panel of

the instrument at regular intervals.

• The bottle collects the condensate that accumulates during defrosting.

Dispose of the contents of the bottle in accordance with laboratory

regulations.

67

Leica CM1950 Cryostat

12. Maintenance and Cleaning

Basically, we recommend UV disinfection (see page 35 for detailed

instructions).

For easy-to-use spray disinfection we recommend Leica Cryofect. (Cryofect

is not available in all countries!)

The cryostat has to be disinfected after each daily use.

Comply with the instructions for use!

The glass anti-roll plate can remain in place during disinfection.

It is not necessary to oil the parts, such as the T-piece on the

microtome base plate, clamping lever etc.

• In case of visible pollution (such as dust), clean the air inlet opening

(Fig. 10, p. 18) of the condenser on the bottom right-hand side of the

instrument using a brush, broom or extraction cleaner in the direction of

the louvers.

12.2 Changing Fuses

• In case of power supply faults, please contact an authorized Leica service

technician immediately.

Do not carry out any repairs on your own as this will invalidate the

warranty.

Repairs may only be carried out by qualified service engineers

authorized by Leica.

Do not turn the instrument on before the cryochamber is

completely dry!

Frost formation!

The front panel and the slit cover of the microtome must be com-

pletely dry before turning on the instrument!

Dry all parts completely before reinserting them in the cryochamber!

68 Operating Manual V 1.1 – 05/2007

12.3 Replacement of the Lamp/UVC Lamp

Turn the instrument off with the automatic power fuse and pull the

power plug, before replacing the lamp!

If the lamp is broken, it must be replaced by the technical service,

as the replacement involves a high risk of injury. Beware of the

metallic mercury in the UV lamp; handle it carefully and dispose of

it properly.

A UVC lamp has an estimated service life of approx. 9,000 hours.

Each on/off switching cycle reduces the lamp life by approx. one hour plus

burning time (30 minutes or 180 minutes respectively).

When both disinfection indicator lights are blinking alternately in control

panel field 1, the UVC lamp must be replaced.

Instructions on how to replace the UVC lamp are provided together

with the replacement lamp (spare part).

12. Maintenance and Cleaning

69

Leica CM1950 – Cryostat

Installation of the new lamp

Type:

OSRAM DULUX L 18W/ 840.

• Hold the lamp in the correct mounting posi-

tion as shown and push it to the left until it

engages in the holder.

• Slightly lift the sliding window (1) holding it by

the grip (2) and pull it out to the front.

1

2

34

Removal of the lamp

• The lamp is mounted behind a glare shield and

therefore not visible.

• Touch the lamp for better orientation.

• Lightly tilt the fluorescent tube down to the left

and pull it out of the clip (3).

• Hold the lamp with both hands and pull it to

the left out of the holder (4).

Only use lamps of the same specifica-

tion!

Fig. 70

Fig. 69

Fig. 68

12. Maintenance and Cleaning

70 Operating Manual V 1.1 – 05/2007

13. Decontamination Certificate (master)

Dear Customer,

Any product which is to be returned to Leica Microsystems or serviced on site, must be cleaned

and decontaminated in the appropriate manner. Leica accepts that it is not possible to decontami-

nate for CJD and its variants and in this instance following current customer safety guidelines is

acceptable. Equipment exposed to CJD samples cannot be returned to Leica Microsystems for

repair.

This certificate, duly completed, must be placed in the unit prior to packaging or handed over to the

service engineer. Packages will not be opened nor servicing commenced until the Company or

service engineer have received a satisfactory certificate. Should returned goods be considered a

hazard by the Company, they will be returned immediately to the customer at his/her expense.

NB: Microtome knives must be in boxes.

AThis equipment has not been in contact with unfixed biological samples.

Yes

BThis equipment has been exposed internally or externally to hazardous materials as

indicated below:

1

Yes No Please provide further detail here:

Blood, body fluids, pathological samples

Other biohazards

Chemicals/substances hazardous to health

Other hazards

2This equipment has been cleaned and decontaminated:

Yes No Please provide further detail here:

If yes, give details of the method:

If no*, please indicate why not:

* Such equipment must not be returned without the written agreement of Leica Microsystems.

Description

Name/Model Fabr. No.

KAT No. Quantity

Tick Box A if applicable. Otherwise please complete all parts of B, providing

further information as requested or appropriate.

71

Leica CM1950 – Cryostat

13. Decontamination Certificate (master)

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17-19

69226 Nussloch, Germany

Phone: ++49 (0) 6224 143 0

Fax: ++49 (0) 6224 143 200

www.leica-microsystems.com

Important - to avoid refusal of shipment:

Place one copy in the unit prior to packaging or hand it over to the service engineer. Customer assumes

all responsibility for the immediate return shipment of articles sent to Leica without proper decontam-

ination documentation.

If you have any further questions, please call your local Leica organization.

Leica Internal Use: If applicable, note corresponding Job and RAN-/RGA-Number:

Job Sheet No.: ____________________

SU Return Goods Authorisation: ____________________

BU Return Authorization Number: ____________________

Signature/Date

Name

Position

eMail

Institute

Department

Address

Phone Facsimile

3

Yes No The equipment has been prepared to ensure safe handling/transportation.

Whenever possible, please use the original transportation case/box.

We herewith declare, in exclusive responsibility, that the

Leica CM1950 Cryostat

was developed, designed and manufactured to conform with the

• Council Directive 2006/95/EC (Low Voltage),

• Council Directive 89/336/EEC, Appendix I (Electromagnetic Compatibility) and

• European council Directive 98/79/EC (IVD)

including their amendments up to the date mentioned below.

The following harmonized standards were applied:

• EN 61010-1: 2001

Safety requirements for electrical equipment for measurement, control and laboratory use -

Part 1: General requirements

• EN 61010-2-101: 2002

Safety requirements for electrical equipment for measurement, control and laboratory use -

Part 2-101: Particular requirements for in vitro diagnostic (IVD) medical equipment

• EN 591: 2001

Instruction for use for in vitro diagnostic instruments for professional use

• EN 61326-1: 2006

Electrical equipment for measurement, control and laboratory use - EMC requirements -

Part 1: General requirements

• EN 61326-2-6: 2006

Electrical equipment for measurement, control and laboratory use - EMC requirements

• EN 61000-3-2: 2000

Electromagnetic compatibility (EMC)

Part 3-2: Limits - Limits for harmonic current emissions

• EN 61000-3-3: 1995 + A1: 2001

Part 3: Limits -

Section 3: Limitation of voltage changes, voltage fluctuations and flicker in public low-voltage

supply systems, for equipment with rated current

≤ 16A per phase

Electromagnetic compatibility (EMC)

• EN 418: 1992 • EN 1037: 1995

Emergency stop equipment. Safety of machinery.

Principles for design. Prevention of unexpected start-up.

In addition, the following in-house standards were applied:

• DIN EN ISO 9001: 2000

Quality management system - requirements.

EC Declaration of Conformity

14. EC Declaration of Conformity

. . . . . . . . . . . . . . . . . . . . . . . . . . . .

Anne De Greef-Safft

President Biosystems Division

Leica Biosystems Nussloch GmbH

Postfach 1120

D-69222 Nussloch

October 11, 2007

73

Leica CM1950 Cryostat

15. Warranty and Service

Warranty

Leica Biosystems Nussloch GmbH guarantees that the contractual product

delivered has been subjected to a comprehensive quality control proce-

dure based on the Leica in-house testing standards, and that the product is

faultless and complies with all technical specifications and/or agreed

characteristics warranted.

The scope of the warranty is based on the content of the concluded agree-

ment. The warranty terms of your Leica sales organization or the organiza-

tion from which you have purchased the contractual product shall apply

exclusively.

Technical service information

If you require technical service or replacement parts, please contact your

Leica sales representative or dealer who sold the product.

Please provide the following information:

• Model name and serial number of the instrument.

• Location of the instrument and name of the person to contact.

• Reason for the service call.

• Date of delivery.

Shutdown and disposal of the instrument

The instrument or parts of the instrument must be disposed of in compli-

ance with the local laws.

Dispose of UV tube separately.

74 Operating Manual V 1.1 – 05/2007

16. People's Republic of China