Vespucci_UG_eBook_(NoSecPack) Agilent2100 Expert User Guide

• About this Manual
  • In this Manual
  • Related Documents
  • How to Use this Manual
    • Online Navigation
    • Layout Conventions
    • Safety Notices, Notes and Tips
• Quick Start
• Looking at 2100Expert
  • Introduction to the Key Features of the 2100expert
  • Starting 2100Expert
  • 2100Expert Work Area
    • Instrument Context
    • Data Context
    • Verification Context
    • Comparison Context
    • Assay Context
    • System Context
  • Closing 2100Expert
• Running and Evaluating Electrophoretic Assays
  • Principles of Nucleic Acid and Protein Analysis on a Chip
  • Preparing and Running an Electrophoretic Assay
    • Selecting an Electrophoretic Assay for a Chip Run
    • Preparing Samples, Reagents, and Chips for Electrophoretic Assays
    • Loading the Electrophoresis Chip into the Bioanalyzer
    • Running an Electrophoretic Assay
    • Entering Chip, Sample, and Study Information
    • Displaying the Measurement Results (Electrophoresis)
    • Cleaning the Electrodes after an Electrophoretic Assay
  • Analyzing and Evaluating the Results of an Electrophoretic Assay
    • Data Analysis: DNA
    • Data Analysis: RNA and Cy5-Labeled Nucleic Acids
    • The RNA Integrity Number (RIN)
      • Examples for RNA Integrity Numbers
      • Computation of the RNA Integrity Number and Signal Anomalies
      • Use Models for the RNA Integrity Number
      • RNA Integrity Number Setpoints
    • Data Analysis: Protein
    • Protein Absolute Quantitation
    • Smear Analysis
    • Changing the Data Analysis
    • Manual Integration
    • Reanalyzing a Chip Data File
    • Comparing Samples from Different Electrophoretic Chip Runs
  • Result Flagging
    • Color Indication
    • How to Use the Form Mode
    • How to Use the Editor Mode
    • Example: Result Flagging
• Running and Evaluating Flow Cytometric Assays
  • Principles of Flow Cytometric Measurements
    • Staining Cells
    • Cell Detection with the Agilent 2100 Bioanalyzer
  • Overview of Flow Cytometric Assays
  • Preparing and Running a Flow Cytometric Assay
    • Selecting a Flow Cytometric Assay for a Chip Run
    • Preparing Samples and Chips for Flow Cytometric Assays
    • Loading the Cell Chip into the Bioanalyzer
    • Running a Flow Cytometric Assay
    • Entering Chip, Sample, and Study Information
    • Displaying the Measurement Results (Flow Cytometry)
  • Analyzing and Evaluating the Results of a Flow Cytometric Assay
    • Using Histograms for Evaluation
      • How to Insert a Marker in a Histogram
      • How to Configure Markers
      • How to Move the Upper and Lower Limits of Markers
      • How to Copy Markers to All Histograms
      • How to Set the Gating Direction (Generic assay only)
      • How to Overlay Histograms
      • How to Set Signal Colors for Overlaid Histograms
      • Displaying the Results of Histogram Evaluations
    • Using Dot Plots for Evaluation
      • How to Add Regions to Dot Plots (Generic Assay only)
      • How to Configure Regions
      • How to Change Position and Size of a Region
      • How to Insert a Region in All Dot Plots
      • How to Work with Gates in Dot Plots
      • Displaying the Results of Regions
    • Evaluating Antibody Staining, Apoptosis, and GFP Assays
      • Evaluating Antibody Staining Assays
      • Evaluating Apoptosis Assays
      • Evaluating GFP Assays
• Working with Chip Data and Assays
  • 2100Expert Data Overview
  • Handling Assays
    • How to Create a Custom Assay
    • How to Modify a Custom Assay
  • Handling Chip Data
  • Organizing, Backing up, and Archiving 2100Expert Data
  • Importing Data
    • Importing Bioanalyzer Files
    • Importing Data Analysis Setpoints
    • Importing Chip, Sample, and Study Information
    • Importing Result Flagging Rules
  • Exporting Data
    • Exporting Chip Run Data
    • Exporting Chip Run Data Automatically
    • Exporting Tables
    • Exporting Graphs
    • Copying Graphs and Tables into the Clipboard
    • Exporting Chip, Sample, and Study Information
    • Exporting Result Flagging Rules
  • Printing Reports
    • How to Print a Chip Run Report
    • How to Turn on and Configure Automatic Printing of Chip Run Reports
  • Configuring Tables
    • Showing and Hiding Columns
• Administering System Functions
  • Configuring 2100expert
    • How to Specify Data File Names and Directories
    • How to Set Run and Result Options
    • How to Set Auto Export Options
    • How to Activate Software Licenses
  • Using Log Books
    • How to Change the Display of the Log Books
    • How to Search the Log Book
• Running Instrument Diagnostics
  • How to Run Instrument Diagnostics Tests
• Performing Verifications
• Products, Spare Parts, and Accessories
• Glossary