RAL DIAGNOSTICS 405000 Tag Reader User Manual 12060 V05 User Guide RAL Stainer EN Emitech

RAL DIAGNOSTICS Tag Reader 12060 V05 User Guide RAL Stainer EN Emitech

USERS MANUAL

The RAL Automated staining systemUser GuideRAL STAINERThe RAL Automated staining systemUser GuideRef:12060-V05 / EN
RAL STAINER2 RAL STAINER User guideBefore use, please read the user guide carefully in order to be able to use the equipment correctly.The user guide must be kept in the immediate vicinity in order to be available for reference at all times.All rights reserved. The reproduction, modification or use of any part of this document in any form is prohibited without the written permission of RAL Instruments. RAL DIAGNOSTICS - Site Montesquieu - 33650 Martillac - FRANCE E-mail: commercial@ral-diagnostics.fr Tel: +33 (0) 557 960 404 - Fax: +33 (0) 557 960 405 www.ral-instruments.com History of changes to the user guide Revision Date Section Software Version 1 03/2012 2.43 2 07/2012 6 2.55 3 08/2012 12 2.55 4 02/2013 - 2.60 5 07/2013 - 2.63Updates available on www.ral-instruments.com.
RAL STAINER3 RAL STAINER User guide1 INTRODUCTION .....................................51.1 RESPONSIBILITY ...................................52 SYMBOLS .........................................62.1 SYMBOLS USED IN THIS GUIDE ........................62.2 DESCRIPTION OF THE SYMBOLS ON THE APPLIANCE .........63 WARRANTY AND WARRANTY LIMITS .....................74 SAFETY INSTRUCTIONS ............................... 84.1 COMPLIANT USE ...................................84.2 WARNINGS .......................................84.3 WASTE ..........................................94.3.1 ELECTRONIC WASTE ..............................94.3.2 BACTERIOLOGICAL AND CHEMICAL WASTE ...............95 DESCRIPTION OF THE APPLIANCE ......................105.1 OPERATING PRINCIPLES ............................135.2 PERFORMANCE ...................................135.3 SAFETY ........................................135.4 SIZE ..........................................135.5 ELECTRIC SPECIFICATIONS .......................... 135.6 ENVIRONMENTAL LIMITS FOR USE .....................145.7 ENVIRONMENTAL LIMITS FOR TRANSPORTATION AND STORAGE 146 STAINING REAGENTS ............................... 157 INSTALLATION ....................................167.1 PACKAGING LIST .................................. 167.2 INSTALLATION ENVIRONMENT ........................177.3 SETTING UP ..................................... 177.4 CONNECTION OF THE INSTRUMENT TO THE MAINS POWER SUPPLY 7.5 HYDRAULIC CONNECTIONS ..........................188 SCREEN DESCRIPTIONS .............................198.1 MEANINGS OF THE SYMBOLS ........................198.2 PAGE DESCRIPTIONS ..............................208.2.1 STAINING PAGE ................................. 208.2.2 SETTINGS PAGE .................................218.2.3 CHANGE SESSION PAGE ...........................238.2.4 INFORMATION PAGE .............................. 248.2.5 ALPHANUMERIC AND NUMBER KEYBOARD ..............259 INITIALISATION ....................................269.1 USER CREATION AND RIGHTS MANAGEMENT ............. 2610 USE ...........................................2910.1 STARTING UP ................................... 2910.2 INSTALLING REAGENTS ............................ 2910.2.1 STAINING KIT ..................................2910.2.2 RINSING SOLUTION .............................30CONTENTS
RAL STAINER4 RAL STAINER User guide10.3 STAINING PROTOCOL .............................3010.3.1 PROTOCOL MODIFICATION ........................3110.3.2 CHANGING THE PROTOCOL ........................3310.4 INSTALLING THE SLIDES ...........................3310.4.1 LOADING THE SLIDE HOLDER ...................... 3310.4.2 PLACE THE SLIDE HOLDER IN THE APPLIANCE .......... 3310.5 STAINING ...................................... 3410.6 CHANGING THE STAINING KIT OR DRAINING ............. 3410.6.1 CHANGING THE STAINING KIT ......................3410.6.2 DRAINING ....................................3510.7 STAND-BY .....................................3510.8 POWERING OFF ..................................3511 TRACEABILITY ................................... 3611.2 LOADING THE TRACEABILITY FILE .....................3612 CLEANING ......................................3712.1 CLEANING .....................................3712.2 HYDRAULIC SYSTEM CLEANING ......................3712.3 ACTIVATED CARBON FILTER .........................3812.4 DECONTAMINATION ............................... 3913 CONSUMABLES AND OPTION EQUIPMENT ...............4013.1 CONSUMABLES .................................4013.2 OPTIONAL EQUIPMENT ............................4014 MAINTENANCE ................................... 4115 AFTER-SALES SERVICES ............................ 4116 TROUBLESHOOTING ...............................4216.1 ERROR MESSAGES ...............................4216.2 WHAT TO DO WHEN ...? ............................4317 SUMMARY OF TIPS ................................4518 INDUSTRIAL PROPERTY ............................ 4619 EC DECLARATION .................................4720 INDEX .........................................48
RAL STAINER5 RAL STAINER User guideThank you for having chosen RAL Stainer.RAL Stainer is intended for the staining of haematological or bacteriological smears.It can be used to autonomously stain 20 slides and returns the slides ready for observation.It recognises the staining kits installed and recommends the protocol to use as well as the renewal of the reagents.It can trace staining carried out using an RFID system.It ensures the complete safety of the user: the user loads and unloads the biological samples without opening the appliance, the residual stain emanations are retained by an activated carbon filter.1.1 RESPONSIBILITYEven if the greatest care has been given to the drafting of this guide, RAL Instruments cannot be held liable by any party in the event of damages caused, or supposed to have been caused, either directly or indirectly, by the failure to carry out the instructions contained in this guide or by the non compliant use of the equipment described below.1 INTRODUCTION
RAL STAINER6 RAL STAINER User guide2 SYMBOLS2.1 SYMBOLS USED IN THIS GUIDERAL Stainer is designed in order to ensure the maximum safety of the user. However, it is necessary to follow the instructions described in this guide and indicated by the following symbols:Attention : Failure to respect the information preceded by this symbol can be dangerous for the user or for the environment ; or cause major damage to the appliance.Warning: This symbol precedes all information covering the correct use of the appliance and a quality diagnosis.Biological risksInformation: This symbol precedes information that may be useful to a user operating the appliance. Tips: This symbol precedes useful advice. 2.2 DESCRIPTION OF THE SYMBOLS ON THE APPLIANCE Biological RisksREF Appliance Reference SN Appliance Serial Number Manufacturer's Address Fabrication Date Operating relative humidity limits Operating temperature limits Recycling of the instrument or return to the distributor Pollution Control Attention, see User Guide Consult the User Guide Medical In Vitro Diagnosis System Security Certification European ComplianceBack sideFront side
RAL STAINER7 RAL STAINER User guide3 WARRANTY AND WARRANTY LIMITSThe duration of the warranty for equipment and manufacturing faults is indicated on your warranty contract. The warranty period starts on the day your RAL Instruments representative commissions the equipment.Failure to respect the safety instructions below or a use which is not compliant with this user guide may use cause the cancelling of the warranty.The warranty does not cover wear parts.
RAL STAINER8 RAL STAINER User guide4.1 COMPLIANT USEr3"-4UBJOFSJTintended for the staining of haematological or bacteriological smears. It operates using RAL Stainer kits from RAL Diagnostics. It is not designed for any other use and cannot be operated using other reagents than those from RAL Diagnostics.r5IFTNFBSTXJMMCFNBEFPOTUBOEBSETMJEFTr3"-4UBJOFSJTTUSJDUMZSFTFSWFEGPSJOUFSJPSVTFr5IFVTFPGUIFBQQMJBODFNVTUDPNQMZXJUIUIFJOTUSVDUJPOTHJWFOJOUIJTHVJEFr5IFDPOEJUJPOTPGDPNQMJBOUVTFBMTPJODMVEFUIFSFHVMBSDMFBOJOHPGUIFBQQMJBODFBOEUIFDBSSZJOHPVUPGmaintenance operations.r5IFVOQBDLJOHJOTUBMMBUJPOBOESFQBJSPQFSBUJPOTNVTUCFDBSSJFEPVUCZBOBVUIPSJTFEUFDIOJDJBOrThe reservoir tank must be always in place. See section 7.3.r5IJTEFWJDFDPNQMJFTXJUI1BSUPGUIF'$$3VMFT0QFSBUJPOJTTVCKFDUUPUIFGPMMPXJOHUXPDPOEJUJPOTthis device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.4.2 WARNINGSr Failure to respect the safety instructions and the use instructions may compromise the user safety.r/FWFS DPOOFDU UIF BQQMJBODF UP B NBJOT QPXFS PVUMFU TVQQMZJOH B WPMUBHF EJGGFSFOU UIBO UIBUindicated on the rear of the appliance. The main supply must not exceed ± 10% of the specified voltage (100-240V). Failure to follow this instruction can cause a fire or an electric shock.r"WPJEDBVTJOHEBNBHFUPUIFQPXFSTVQQMZDBCMF%POPUQMBDFBOZBQQMJBODFTPOJU%POPUUVHon the cable (to disconnect the appliance for example). Failure to follow these instructions can cause a short circuit or a disconnection that could, in turn, cause a fire or an electric shock.rBefore connecting the product to the mains, check that the electrical installation of the building complies with national installation regulations. rDo not use a power cable of a length greater than 3m.r/FWFSUPVDIUIFFMFDUSJDDJSDVJUTPSPUIFSQBSUTMPDBUFEJOTJEFUIFBQQMJBODF5IFSFJTBSJTLPGelectric shock, especially with damp hands.r5IFMJUIJVNCBUUFSZDPOUBJOFEJOUIFFMFDUSPOJDDBSEMPDBUFEJOUIFMFGUSFBHFOUESBXFSPGUIFEFWJDFcan be replaced by a battery with the same characteristics (CR1220 3v). If not, it may cause an explosion. Throwing away used batteries according to your laboratory practises.r*OUIFFWFOUPGVOVTVBMTNPLFCFJOHFNJUUFECZUIFBQQMJBODFJNNFEJBUFMZTXJUDIJUPGGVTJOHUIFmain switch and unplug the mains power supply cable. Contact the RAL Instruments post-sales service.r*UJTQSPIJCJUFEUPGPSDFUIFDPWFSPQFOJOHTUIFTMJEFESBXFSTPSUIFSFBHFOUESBXFSTCPUIXIFOoperating and when not operational. r*UJTQSPIJCJUFEUPGPSDFUIFSPUBUJOHBSNCPUIXIFOPQFSBUJOHBOEXIFOOPUPQFSBUJPOBMr8FBSMBCPSBUPSZHMPWFTBOE B MBCPSBUPSZ DPBU XIFO VTJOH UIF BQQMJBODFBOE JUTSFBHFOUT 5IFdangers relative to each reagent are indicated on their respective packaging or see section 6. In the event of an accident, refer to the relevant safety data sheets. rAll biological samples, all effluents and all used consumables should be considered to be potentially dangerous. Once the appliance is no longer used, the user should disinfect and wash his/her hands. rThe contact between contaminated hands and the appliance can cause the contamination of the appliance. In this case, decontaminate the appliance according to current laboratory best practises. See also section 12.2.r(FOFSBMMZNBLFTVSFUIBUDVSSFOUMBCPSBUPSZCFTUQSBDUJTFTBSF used.The guarantee is not responsible for any changes or modifications not expressly approved by the party responsible for compliance. Such modifications could avoid the user's authority to operate the equipment.4 SAFETY INSTRUCTIONS
RAL STAINER9 RAL STAINER User guide4.3 WASTE4.3.1 ELECTRONIC WASTEThis symbol is applicable in European countries. It indicates, in compliance with the 2003/108/EC directive relating to electric and electronic waste, that electric and electronic objects are subject to selective waste collection by the company that brought them onto the territory. Contact your RAL Instruments representative.Outside the European Union, refer to current local or national regulations.4.3.2 BACTERIOLOGICAL AND CHEMICAL WASTEBefore disposing of biological samples and/or chemical reagents, consult the reagent safety instructions.Generally, make sure that current laboratory best practises are used.
RAL STAINER10 RAL STAINER User guide5 DESCRIPTION OF THE APPLIANCEOpen front face viewLeft view Right viewSide view with drawers openTop view with drawers open
RAL STAINER11 RAL STAINER User guideTouch screen interfaceStandby buttonStaining armConnectors liquid with level detectionWaste outlet and rinsing liquidSlide loading/unloading drawersAnd drying zone (x2)Autonomy of 1 to 20 slides(2 ten-slide slide holders)Staining stations with sealed lid (x4)Reagent loading drawers Protective coverDraining nozzle
RAL STAINER12 RAL STAINER User guideOn/Off or0/1 switchActivatedcarbon filterPeristaltic pumpfor effluent suctionPower supply inputConnections for the rinsing liquid and the waste outletConnections for the rinsing level and the waste outlet levelUSB port for downloading traceability information
RAL STAINER13 RAL STAINER User guide5.1 OPERATING PRINCIPLESRAL Stainer is an automatic bath staining appliance.A arm which follows a circular and vertical path successively:- Hooks the slide holder installed by the user;- Brings the slide holder into each staining tank and then into the rinsing tank depending on the recorded protocol;- Returns the slide holder to its initial position.- The slides are then dried.Staining is complete.5.2 PERFORMANCEr-$%UPVDITDSFFOr$BQBDJUZYTMJEFTrTUBJOJOHTUBUJPOTrSJOTJOHTUBUJPOrMPBEJOHVOMPBEJOHESBXFSTGPSUPTMJEFTFBDIXJUIBWFOUJMBUPSUPESZUIFTMJEFTBUUIFFOEPGUIFDZDMFr6QUPTUBJOJOHQSPHSBNNFTJONFNPSZrVTFSTUIBUDBOCFQBSBNFUFSFEGPVSMFWFMTPGBVUIPSJTBUJPO"ENJOJTUSBUPS&YQFSU6TFSBOE.BJOUFOBODFr3'*%4ZTUFNAutomatic recognition of kits and corresponding staining protocol optimised by RAL Diagnostics.Control of the batch, the expiry date, the staining kit autonomy and the activated carbon filter replacement.r1PMZWBMFOUVTFIt is possible to switch from one kit to the other, from one application to another: RAL Stainer keeps the data about all the kits being used in memory.r4UBJOJOHUSBDFBCJMJUZøIt is possible to print a report giving the correlation of work dates, users, staining and the product batch used.5.3 SAFETYVapour emanation is limited because the loading/unloading of the reagents and slides is carried out using drawers.Each tank is closed using a sealed lid inside the appliance.The cover need not be opened other than for cleaning operations.RAL Stainer has a ventilation system which uses an activated carbon filter to evacuate all possible residual vapour (Warning when the filter needs to be changed).The arm is fitted with a level sensor which adjusts the slide immersion height depending on the liquid remaining in the containers.The draining of the solutions is carried out by the appliance itself thanks to a peristaltic pump which directs towards the waste bin (10 L), thus avoiding dangerous handling.The waste bin level will be automatically detected in order to avoid any overflow.The level of rinsing liquid will be automatically detected to avoid launching staining without rinsing liquid. Drainage holes and a reservoir tank can collect liquids in case of leak. 5.4 SIZEWeight: 28 kg Diameter: 42.5 cm Height: 62.4 cm5.5 ELECTRIC SPECIFICATIONSVoltage: 100-240V/ ~ 50-60Hz. Overvoltage category 2Operating power: 150 VA
RAL STAINER14 RAL STAINER User guide5.6 ENVIRONMENTAL LIMITS FOR USETemperature: from 18 to 30°CHumidity: from 20 to 85%RAL Stainer is strictly reserved for interior use. It is not recommended to use this device in a location with an altitude more than 3 000 meters and in an environment having a pollution degree superior than 2.5.7 ENVIRONMENTAL LIMITS FOR TRANSPORTATION AND STORAGETemperature: from -10 to 55°CHumidity: from 5 to 85%
RAL STAINER15 RAL STAINER User guideOnly RAL Diagnostics staining reagents can be used in the RAL Stainer.Staining kits available:Some reagents may occur a danger. Refer to the staining kit packaging and reagents MSDS.Refer to the staining protocol for the use of each staining kit, for the attempt performances, for the quality control and the standard staining protocol.6 STAINING REAGENTSField ofactivity Kit description Use ReferenceNumber ofreusableslidesNumber of days after opening = BBDExpiryHaematologyBacteriologyBacteriologyBacteriologyKit RAL Stainer MCDhKit RAL Stainer Gram-HuckerKit RAL Stainer Gram-NicolleKit RAL Stainer 555Staining kit forblood and bone marrow smearsStaining kit forGram + and Gram - bacteriaStaining kit forGram + and Gram - bacteriaFast staining kitfor cytobacteriology360200-0000 360210-0000360220-0000360250-0000500 slides500 slides500 slides300 slides21 days14 days14 days14 days18 months24 months24 months24 monthsXiEthylic Alcohol, IsopropanolF, XnEthylic Alcohol, Crystal VioletFEthylic AlcoholF, TMethanolDangerBacteriologyBacteriologyKit RAL Stainer Fluo-RALKit RAL Stainer Cold ZNAuramine staining kitfor mycobacterium testsCold fuchsine staining kitfor mycobacterium tests360230-0000360240-0000300 slides500 slides7 days14 days12 months12 monthsF, CEthanol, PhénolF, CEthanol, Phénol
RAL STAINER16 RAL STAINER User guideThe RAL Stainer must be installed by a technician approved by RAL Instruments.7.1 PACKAGING LISTCheck that all the items below are contained in the RAL Stainer package:Description QuantityRAL Stainer 1Power supply (L=2m) 1RAL Stainer 10 L Jerrycan 2Hydraulic cane for effluents 1Hydraulic cane for rinsing liquid 1Reservoir tank 1Pack of Slide-holder RAL Stainer (x3) 1Rinsing Station RAL Stainer 1Filter Cartridge RAL Stainer 2Stylus 1RAL Stainer CD 1RAL Stainer Quick Start 1Label sheets 20RAL Stainer USB Stick 1If one of the items is damaged or missing, contact your RAL Instruments representative.7 INSTALLATION
RAL STAINER17 RAL STAINER User guide7.2 INSTALLATION ENVIRONMENTr4UPSFUIFJOTUSVNFOUJOBMPDBUJPOXJUIJOUIFFOWJSPONFOUBMPQFSBUJOHMJNJUTr5IFBQQMJBODFNVTUCFQMBDFEPOBTUBCMFGMBUBOEDMFBOUBCMF"WPJETIPDLTBOEWJCSBUJPOTr$POTJEFSJOHUIFXFJHIUPGUIF3"-4UBJOFSLHVTFBTVGGJDJFOUMZTPMJEUBCMFr1MBDFUIFBQQMJBODFDMPTFUPBNBJOTQPXFSPVUMFUBOEBSVOOJOHXBUFSUBQJGZPVIBWFTFMFDUFEUIFSVOOJOHwater connection option.r$POTJEFSJOHUIFTJ[FPGUIF3"-4UBJOFSDN)DNQMBOUPVTFBTQBDFPGYDNGPSthe appliance installation, use and maintenance. Avoid placing the appliance behind a window in order to limit the heating of the reagents under the sun's rays. If the appliance has been stored at temperatures outside the environmental usage limits, it must remain at room temperature for 1 hour before powering up.Avoid using the appliance in close proximity to a chemical reagent storage area or in an atmosphere containing emanations of dangerous gases.Make sure the on/off switch and the mains power outlet remain accessible.If you have to move the instrument, handle it carefully by lifting it up by the bottom, like showing the opposite picture7.3 SETTING UPPlace the RAL Stainer on a flat and stable surface, in a sufficiently wide space, as described in section 7.2.Place the two supplied containers beneath the table.To eliminate any risk of error in the use of the appliance, mark each supplied container with the supplied labels in order to assign a function to each one: Effluent Container and Rinsing liquid container.The standard containers supplied with the RAL Stainer can be replaced by autoclave compatible containers. Contact your RAL Instruments representative.The supplied containers can also be replaced using your usual containers. However take care over the sealing of the container as it is not certain that the supplied cover fits your container.Rayon vrai 200 mm424 mm250 mm421 mm624 mm460 mmwwiddddibibddiipplied container with the CCntainer and Rinsing liquid ee Réf.: 12094-V04 Rév.: 09/2013RAL STAINERr fffffffffffffff Réf.: 12094-V04 Rév.: 09/2013RAL STAINERRinse labelWaste labelSlide the reservoir tank below the machine on the back until it is blocked.The reservoir tank must always be empty. If it is full, because of the flammability hazard of some products, remove it by sliding it gently, then empty it and call your service engineer.
RAL STAINER18 RAL STAINER User guide7.4 CONNECTION OF THE INSTRUMENT TO THE MAINS POWER SUPPLYOnly use the power supply supplied with the appliance.Using the supplied power cable, connect the RAL Stainer to an alternating current power outlet nearby.The appliance operates in the voltage range 100V to 240V, 50-60Hz7.5 HYDRAULIC CONNECTIONSConnect the Effluent container and the rinsing liquid container to the right hand part of the appliance.1. Introduce the tube and the level sensor connected to the red cover into the waste container and screw the cover to hermetically seal the container.2. Introduce the tube and the level sensor connected to the blue cover into the rinsing container and screw the cover to hermetically seal the container.3. Connect the tubes to the corresponding couplers on the appliance taking care to respect the colour codes: Blue: Connection of the rinsing liquid container Yellow: Connection of the effluent container4. Connect the electric cables using the male jack connectors to the appliance's female male connectors in any order. The level sensors do not have any specific connectors.power outlet120/240V, 50-60HzRinsing containertubeRinsing leveland waste levelconnectionsWaste containertube
RAL STAINER19 RAL STAINER User guide8 SCREEN DESCRIPTIONSRAL Stainer has a simple and user friendly interface, and a touch screen.8.1 MEANINGS OF THE SYMBOLS Validate Cancel Go to pagew Staining Settings Change session InformationUnlock the slide drawer Launch staining Halt staining Soak Rinse Mix Dry Drainwwww
RAL STAINER20 RAL STAINER User guideRAL STAINERProgress of stainingStaining in progressDrying in progressAvailable zoneName of the staining protocol usedName of the staining kitControl panel of the two work zoneError or information message areaRemaining staining timeNumber of staining cycles remainingDrawer statusTo return to this page, press the button 8.2 PAGE DESCRIPTIONS8.2.1 STAINING PAGEThe Staining page is the page that is always displayed by default. Staining is launched from this page.
RAL STAINER21 RAL STAINER User guide8.2.2 SETTINGS PAGE9 functionsTo access this page, Presentation of the functions:RAL STAINERSETPROTOCOLLOAD/CHANGEREAGENTSEMPTYREAGENTSUSER RIGHTSMANAGEMENTLANGUAGEDATE / HOURMAINTENANCEOPENCOVER LIDADJUSTPROTOCOLNameADJUSTPROTOCOLScreen FunctionUsed to access the protocol modification page and record up to 9 modified protocols modified from standard protocols RAL STAINERyesmin:sec1no1 Reagent name2 Reagent name3 Reagent name4 Reagent namemin:sec
RAL STAINER22 RAL STAINER User guideNameSETPROTOCOLNo specific screen, PLC actionNo specific screen, PLC actionScreen FunctionUsed to access the list of recorded protocols and switch from one to the next.Used to open the sealed lids of the staining stations and the reagent loading side doors in order to load a new staining kit or replace a staining kit.Used to drain the reagents in order to remove the containers in complete safety when the staining kit has expired or is out of service, or when the user requires.Used to open the cover for a weekly interior clean.Used to record the users with a level of rights to be selected.RAL STAINERRAL STAINEREMPTYREAGENTSRAL STAINERUTILISATEURUSEREXPERTADMINISTRATORINSTALLERLOAD/CHANGEREAGENTSEMPTYREAGENTSOPENCOVER LIDUSER RIGHTSMANAGEMENT
RAL STAINER23 RAL STAINER User guideName Screen FunctionUsed to select the operating language; French, English or German.Used to update the date and time on the machine for use with the traceability monitoring.Only accessible to authorised persons. Used to check the appliance's operating steps and to operate the hydraulic system.RAL STAINERFrançaisEnglishDeutschࡄ੶Português᪥ᮏேEspañolItalianoRAL STAINER0123456789H2ONS-+-+-+-+6T1T2T3T4C1C2C3C4L1L2VRerasetraceerasemem8.2.3 CHANGE SESSION PAGEThe Change session page is used to change users while operating.LANGUAGEDATE / TIMEMAINTENANCE
RAL STAINER24Fill in the codeand press on or . Four authorisation levels are defined: Installer, Administrator, Expert and User.To access this page, press 8.2.4 INFORMATION PAGEThe information page gives access to:- The information on the staining kit being used: Kit batch number and remaining number of cycles;- The information about your staining appliance: serial number and software version (GUI software version/Motor software version/ RFID software version)- Access to information on the activated carbon filter: the remaining time of use- Information on the user currently working and the list of all users.To access this page, press This information can be useful when you contact your RAL Instruments representative for questions about the use of your RAL Stainer appliance or your RAL Stainer staining kit.RAL STAINERA B C D E F G H IJ K L M N O P Q RS T U V W X Y Z-SpaceCODE :GHIIFFGGHH0123456789RAL STAINERPROFILESINITINFOREAGENT KIT INFORMATION Loaded kit : --- Remaining cycles : 0MACHINE INFORMATIONS Serial number: 0000 20/02/2012 15:09:31 Software version : 02:41 / 02:14 / V305 Filter autonomy : 61hPROFILES Current session : XXXRAL STAINER User guide
RAL STAINER25 RAL STAINER User guide8.2.5 ALPHANUMERIC AND NUMBER KEYBOARDThese dialogue pages are used in several parts of the interface.They are used to enter numbers from 1 to 9, letters, add a space [Space] or a hyphen [-].The key is used to cancel entered characters.On the number page, the cursor passes automatically from minutes to seconds.Then press : or . RAL STAINERA B C D E F G H IJ K L M N O P Q RS T U V W X Y Z-SpaceGHIIFFGGHH0123456789RAL STAINER0123456789
RAL STAINER26 RAL STAINER User guide9.1 USER CREATION AND RIGHTS MANAGEMENTTurn on the appliance using the 0/1 switch located behind the instrument.Enter the user code created with your RAL Instruments representative during the installation, On first use, you must record the users and assign them access rights. The user code will then suffice to start up the machine.Four authorisation levels can be used:9 INITIALISATIONType Screen RightsUser level This level makes it possible to use the machine and to load protocols that have been defined by an Expert or Administrator user.USER RAL STAINERSETPROTOCOLLOAD/CHANGEREAGENTSEMPTYREAGENTSUSEUSEUSEUSERRRRRRR RIGHIGHIGHIGHTSTSTSTSMANMANAGEAGEMENMENTTLANGUAGELANLANLANGUAGUAGUAGEGEGEDATE / HOURDATDATE/E/HOHOURURMAINTENANCEMAIMAIMAINTENTENTENANNANNANCECECEOPENCOVER LIDADJADJADJADJUSTUSTUSTUSTPROPROTOCTOCOLOLUser level + creation and modification of staining protocols.This level makes it possible to use the machine and to modify the settings for a protocol or to create a new protocol.EXPERT RAL STAINERSETPROTOCOLLOAD/CHANGEREAGENTSEMPTYREAGENTSUSEUSEUSEUSERRRRRRR RIGHIGHIGHIGHTSTSTSTSMANMANAGEAGEMENMENTTLANGUAGELANLANLANGUAGUAGUAGEGEGEDATE / HOURDATDATE/E/HOHOURURMAINTENANCEMAIMAIMAINTENTENTENANNANNANCECECEOPENCOVER LIDADJUSTPROTOCOLRAL STAINERA B C D E F G H IJ K L M N O P Q RS T U V W X Y Z-SpaceCODE :GHIIFFGGHH0123456789
RAL STAINER27 RAL STAINER User guideType Screen RightsAdministrator level + access to the maintenance pageThis level gives access to all the functions of the machine. It is reserved for persons authorised by RAL Instruments.INSTALLER RAL STAINERSETPROTOCOLLOAD/CHANGEREAGENTSEMPTYREAGENTSUSER RIGHTSMANAGEMENTLANGUAGEDATE / HOURMAINTENANCEOPENCOVER LIDADJUSTPROTOCOLa. On the settings menu , pressThe user list page appears.b. Select a location. 10 users can be defined.If you use a location that is already in use, the new record will overwrite the previous record.USER RIGHTSMANAGEMENT RAL STAINERExpert level + Rights management + some functions of maintenance screenThis level gives access to all settings except for the Maintenance page.Only an Administrator can create a new user and define his/her rights. Authorized functions on the maintenance screen: operating arm movements, activation of water pump and peristaltic pump for effluents, trace file erasing.ADMINISTRATORRAL STAINERSETPROTOCOLLOAD/CHANGEREAGENTSEMPTYREAGENTSUSER RIGHTSMANAGEMENTLANGUAGEDATE / HOURMAINTENANCEOPENCOVER LIDADJUSTPROTOCOL
RAL STAINER28 RAL STAINER User guided. Enter the user identification code and validateIt is recommended to choose a mnemonic type code, the appliance cannot remind the user of the code.e. Choose the level of rights to associate to the user At all times it is possible to change users by clicking on the icon See section 8.2.3.A strict use of this tool makes it possible to enrich traceability related to the use of the machine.RAL STAINERA B C D E F G H IJ K L M N O P Q RS T U V W X Y Z-SpaceCODE :GHIIFFGGHH0123456789RAL STAINERUTILISATEURUSEREXPERTADMINISTRATORINSTALLERRAL STAINERA B C D E F G H IJ K L M N O P Q RS T U V W X Y Z-SpaceNAME :GHIIFFGGHH0123456789c. Enter the user name and validate
RAL STAINER29 RAL STAINER User guide10.1 STARTING UPTurn on the appliance using the 0/1 switch located behind the instrument.The following page is displayed:Enter the user code. Validate10.2 INSTALLING REAGENTS10.2.1 STAINING KITIf, on start-up, the appliance does not detect a staining kit, it will request one to be inserted.1. Press on page 2. The appliance opens the lids on the reagent housings and the reagent loading doors.3. Break the protection seal and place the closed containers from your staining kit using the numbers on the labels to place them at the corresponding location within the instrument. Carefully remove the container caps and keep them. Take off the tamper-proof seal.10 USERAL STAINERA B C D E F G H IJ K L M N O P Q RS T U V W X Y Z-SpaceCODE :GHIIFFGGHH0123456789RFID ReaderSpecial precautions for bottle 1:Bottle 1 contains the RFID chip. This chip must be opposite the RFID reader (located to the left of the left reagent drawer) in order to be read. If we consider that the front face of the bottle is the labelled, curved face, this face must be placed opposite you, thus the chip will be correctly read by the appliance. In the event of an error, the "Tag error" message will be displayed. This can be corrected by turning bottle 1.LOAD/CHANGEREAGENTSRFID chipFront facebottle
RAL STAINERRAL STAINER User guide30Removing the caps from the bottles once they have been placed in the appliance avoids any dangerous handling such as the spilling of the reagent.If the kit is new, turn the caps a quarter turn to break the seals.4. Then gently close the reagent loading drawers until you hear a "click"as they lock.It is important that the necks of the bottles be free from reagent so that the level sensor can adjust the soaking height of the slides in the reagents. Remove the tamper-proof seal, the can disturb the arm movement.5. The appliance closes the covers of the reagent housings.RAL Stainer identifies the staining kit that was placed in the appliance and proposes a "Standard" protocol.It is possible to vary the protocol settings (time, mixing, rinsing). See section 10.3. This function LOAD/CHANGEREAGENTS can also be used to change staining kits during their use.10.2.2 RINSING SOLUTIONFill the container reserved for this use with the adequate rinsing liquid for the stain being used. Refer to the staining kit instructions.Adapt the cap with the suction cane to the rinsing container.In the case of a low consumption of rinsing water, RAL Instruments recommends the regular replacement of rinsing water in order to prevent the proliferation of bacteria and to clean the rinsing liquid container weekly. See section 12.RAL Stainer permanently checks the level of the rinsing liquid and notifies you when the level becomes critical.10.3 STAINING PROTOCOL10 protocols can be memorised by the appliance.The protocols proposed by default are protocols optimised by RAL Diagnostic in collaboration with expert laboratories.Due to the complex actions of the stainers, it is necessary to establish standard staining conditions to ensure good staining quality and reproduction.We therefore advise to test and assess the protocols tested by RAL Diagnostics before making any changes to the protocol settings.RAL STAINERRFID Reading
RAL STAINER31 RAL STAINER User guide10.3.1 PROTOCOL MODIFICATIONRAL Stainer identifies the staining kit that was placed in the appliance and proposes a “Standard” protocol.It is, however, possible to vary the protocol settings (time, mixing, rinsing).On page , ADJUSTPROTOCOLThis function is only accessible to Administrator and Expert users.See section 9.1.Main settingsIn the event of an error, returns to the previous screen. Indicate whether you wish to drain the rinsing liquid after each rinse. A single push on the key is used to switch from yes to no.Indicate the number of rinses after each tank.Pressing this key displays another screen used to define the rinsing parameters. See next section.A single push on the key is used to switch from yes to no.Select "Yes" if you wish to agitate the slides in reagent. If you select "No" the slide holder will not move during soaking.Press the time bar to change the soaking time in tank.RAL STAINERyesmin:sec1no1 Reagent name2 Reagent name3 Reagent name4 Reagent namemin:secLoaded reagentsDipping timeDrying timeSlides shakingNumber of rinsingRinsing stationdrainingTo modify a setting, yesmin:sec1no.
RAL STAINER32 RAL STAINER User guideRinsing3 parameters :DrainingRAL Stainer makes it possible to limit the volume of your effluents. You choose whether to drain the rinsing liquid after each dip. If you choose never to drain the rinsing liquid during a cycle, RAL Stainer will request the draining frequency by the question : "How many cycles before draining rising water?" Enter the value.RAL Instruments recommends draining the rinsing tank after each soaking in order to optimise the quality of the staining.DryingSelect the drying time with the numeric keypad.When all the settings are correct, .Give a name to the defined protocol, .Select a location to which to save your new protocol on the protocol list page.The selection of a location that has already been used will overwrite the protocol that was previously at this location.It is impossible to record a new protocol in location 1 which is reserved for the protocol proposed by RAL Diagnostics. It is possible to return to this protocol at any time.RAL STAINERA B C D E F G H IJ K L M N O P Q RS T U V W X Y Z-SpacePROTOCOL NAME :GHIIFFGGHH0123456789RAL STAINERRAL STAINERMinNumber of rinsesSec0123456789Define whether you require movement during rinsing.Define rinsing time with the numeric keypad.Define the number of rinses by pressing on the left and right arrows.i00min 00s
RAL STAINER33 RAL STAINER User guide1. Turn the slide holder and free the jaw by placing the knob on the O "Open" position.2. Place up to ten slides (identification maintained).3. Block the jaw by moving the knob to the C "Close" position.10.3.2 CHANGING THE PROTOCOLOn page , SETPROTOCOLThis function is available to all users. See section 9.1.The parametered protocol list is displayed, press on the protocol to be changed to load it.The last used protocol remains the active protocol after all appliance off/on operations.10.4 INSTALLING THE SLIDES10.4.1 LOADING THE SLIDE HOLDERPlace from 1 to 10 slides in the slide holder. Always make sure that the slides are correctly placed and aligned.10.4.2 PLACE THE SLIDE HOLDER IN THE APPLIANCEOn page , for unlocking the slide loading drawer.Place the slide holder correctly on its support in the drawer(s).Close the drawer(s) until the "click" indicates they are locked.During staining, it is possible to load a second slide holder or to remove an awaiting slide holder to add or change a slide.RAL STAINER
RAL STAINER34 RAL STAINER User guide10.5 STAININGStaining is launched from the staining page, gives access to this page from any other page. Once the protocol has been selected and the slides placed, to launch the staining.The appliance moves into action, the arm picks up the slide holder. Staining begins.To halt an ongoing staining, .At the end of a cycle, unlock the drawer. Your slides are ready to be examined. If two stainings are launched, the arm will begin the second staining after having dropped the first slide holder back to its initial position. Before the second cycle begins, you can retrieve an awaiting slide holder to add or change a slide by pressing on the lock button.You can retrieve the first batch of slides after drying while the second batch is being stained.The slide holder can be retrieved while drying is in progress. 10.6 CHANGING THE STAINING KIT OR DRAINING10.6.1 CHANGING THE STAINING KITThis is a useful function if you wish to use the appliance for several staining operations.Press LOAD/CHANGEREAGENTS on page .The arm opens the lids, the reagent drawers open.Put the caps back onto the bottles before removing them.Remember to store the bottles away from light.Then insert the bottles for the new staining kit and close the reagent drawers.The appliance is ready to operate using the new staining kit, no cleaning cycle is required.Name of the staining protocol usedName of the staining kitControl panel of the two work zoneError or information message areaRemaining staining timeNumber of staining cycles remainingDrawer statusProgress of stainingStaining in progressDrying in progressAvailable zoneRAL STAINER
RAL STAINER35 RAL STAINER User guideThe appliance can manage several staining kits in parallel, it keeps the data for each kit in memory: the autonomy and the customised protocols for this kit reference. 10.6.2 DRAININGThe RAL Stainer will systematically drain the reagents being used if: EMPTYREAGENTS- the kit has expired,- the autonomy (cycles or slides) has been exceeded,- the level of one reagent is lower. The effluents are rejected into the appliance's waste container, the reagent drawers are opened to allow to remove the empty bottles.The bottles must be disposed of using the decontaminated waste channel.If the expiry date after opening is passed, the RAL Stainer will advise you to drain the staining kit with the message: "Warning BBD expired".On page , EMPTYREAGENTS.These safety mechanisms have been included for quality assurance reasons. We advise you to follow the RAL Stainer recommendations.10.7 STAND-BYAt the end of your working session, simply place the appliance on standby by using the standby button on the top of the machine. The RAL Stainer can be restarted by simply pressing the same button again.10.8 POWERING OFFWhen a long period of inactivity is foreseen, prefer powering off the machine to placing it on standby.In this case press the O/1 switch at the back of the appliance.On/Off or0/1 switchgyStandby button
RAL STAINER36 RAL STAINER User guideThe traceability file will indicate:- Dates of each cycle- User- Staining kit name and batch- Dyeing compliant Yes/No with error message. See section 16.1.- Staining protocol11.1 USING THE TRACEABILITY SOFTWAREInsert the supplied CD into your computer.Execute the file "Trace.exe" Follow the instructions.The CD will install software which will enable you to decrypt the traceability file that can be retrieved from the RAL Stainer.11.2 LOADING THE TRACEABILITY FILE1. Create a new file on the supplied USB stick called "TRACE.txt" (capital letters) using the windows Notepad. 2. Turn off the appliance and insert the USB sticksupplied with the appliance in its location.3. Turn the machine back on: - the diode flashes. - 3 Beeps indicate the end of the download.4. Open the file on the USB stick named "TRACE.txt" using your traceability software "Trace.exe" on your computer.11 TRACEABILITY
RAL STAINER37 RAL STAINER User guide12 CLEANING12.1 CLEANINGOn page , OPENCOVER LID gives access to the inside of the machine.Basic weekly surface cleaning is sufficient with a wet cloth.You can use for exemple Ethanol 70°.Clean:- The slide drawers- The slide holders- The inside and outside surfaces- The rinsing tank Procedure of rinsing station releaseNever pour liquid onto the appliance, it could damage the electronic components.Do not use solvents on the screen and the cover lid.In the event of accidental spillage of reagents, turn off the appliance as quickly as possible and clean using a cloth and the appropriate solvent. In all cases limit the dose of solvent.12.2 HYDRAULIC SYSTEM CLEANINGThis procedure should be done weekly in order to prevent the bacteria proliferation.Equipment must be switched on.Rinsing and draining circuits must be connected to the equipment.Decontamination solution advised :- Bleaching water with 2,6 % of active chlorine (available in commerce)- Solutions like Stereanios of the Laboratoires Anios or Elusept of Pierre Fabre Santéa. Clean the rinsing liquid container with a decontamination solution in order to prevent the proliferation of bacteria on the container bottom. b. Fill the rinsing liquid container with approximatively 500 ml of decontamination solution.c. Deep the rinsing cane in the container filled with the disinfectant solution.RAL STAINERSETPROTOCOLLOAD/CHANGEREAGENTSEMPTYREAGENTSUSER RIGHTSMANAGEMENTLANGUAGEDATE / HOURMAINTENANCEMAINMAINMAINTENATENATENANCENCENCEOPENCOVER LIDADJUSTPROTOCOL1. Free the rinsing tray by giving a quarter turn to the screw2. Lift the rinsing nozzle and pivot it lightly3. Remove the rinsing nozzle4. Remove the rinsing tank
RAL STAINER38 RAL STAINER User guide12.3 ACTIVATED CARBON FILTERThe RAL Stainer recommends the changes of the activated carbon filter cartridges.The life span is calculated using the RAL Stainer operating times.RAL Stainer will give you an advertising message before the necessity of the filter-cartridge change: "The activated carbon filter has expired: have you replaced it?"The filter-cartridges are housed on the left of the machine.1. Open the cover lid2. Unscrew the two screws by a quarter turn3. Remove the used filter cartridge4. Fit a new filter-cartridge5. Re-screw the two screws by a quarter turn d. On page , MAINTENANCE.e. Switch on the rinsing pump in order to decontaminate the rinsing circuit : H2O. Switch off the rinsing pump by pressing H2O again.Be careful, pump doesn’t stop automatically.Fill the rinsing tank until to have the level at 1 cm from the rim.f. Clean the waste circuit by switching on the draining pump, , and press again this touch when the tank is empty in order to switch off the pump.Repeat at least 3 times steps “e” and “f” in order to decontaminate perfectly the waste circuit.g. Rinse the rinsing liquid container with your rinsing solution. h. Deep now the rinsing cane in the rinsing solution.Repeat again by 3 times (at least) steps “e” and “f” with the rinsing solution of staining in order to eliminate the disinfectant solution previously used.H2ONS-+-+-+-+6T1T2T3T4C1C2C3C4L1L2VRerasetraceerasemem6. On page , set the cartridge life span counter to zero by pressing RAZRAL Stainer filter cartridges are available from your RAL Diagnostics reseller.RAL STAINERPROFILESINITINFOREAGENT KIT INFORMATION Loaded kit : --- Remaining cycles : 0MACHINE INFORMATIONS Serial number: 0000 20/02/2012 15:09:31 Software version : 02:41 / 02:14 / V305 Filter autonomy : 61hPROFILES Current session : XXX
RAL STAINER39 RAL STAINER User guide12.4 DECONTAMINATIONAll biological samples should be considered to be potentially dangerous. The contact between contaminated hands and the appliance can cause the contamination of the appliance. Before operation of decontamination, unplug the mains power supply cable.Use the adapted personal protective equipment : laboratory gloves, laboratory glasses and a laboratory coat. Decontamination solution advised:- Bleaching water with 2,6% of active Chlorine (available in the commerce)- Solutions like Stereanios of the Laboratoires Anios or Elusept of Pierre Fabre SantéIf your laboratory procedures require it:- the reagent bottles can be decontaminated before being disposed of;- the appliance itself and its accessories (slide-holder, rinsing station) can be decontaminated using air or manual cleaning. Use a decontamination solution and scrub using a clean cloth soaked in the disinfecting BHFOUBMMUIFBQQBSBUVTQBSUTøTVSGBDFTESBJOJOHOP[[MFIZESBVMJDTZTUFN1MFBTFTFFTFDUJPOBOE12.2.- The effluents and their containers are to be eliminated and/or decontaminated in compliance with current laboratory best practises. The procedure will depend on the type of effluent container you are using. RAL Stainer 10L jerrycan: decontamination using air or manual cleaning Autoclave compatible container: resists up to 135°CIn all cases respect the laboratory best practises applied by your laboratory.The use of organic solvents is under the responsibility of the user and may cause the discolouring of certain parts of the appliance as well as the alteration of some plastic parts.
RAL STAINER40 RAL STAINER User guide13.1 CONSUMABLES13.2 OPTIONAL EQUIPMENT13 CONSUMABLES AND OPTION EQUIPMENTConsumable namePack of slide-holder RAL Stainer (x3Filter cartridge RAL StainerRinse station RAL StainerRAL Stainer 10L jerrycanConsumable nameAutoclavable 10L jerrycanConnection systemto the mains water networ
RAL STAINER41 RAL STAINER User guideMaintenance must be carried out by a technician approved by RAL Instruments.For the first year following the purchase of your appliance, the maintenance is ensured by your RAL Instruments representative.Beyond this period you can subscribe to a maintenance contract. Contact your RAL Instruments representative.If your RAL Stainer should be return to your RAL Instrument representative workshop, it must be decontaminated.The decontamination declaration joined with your apparatus should be well filled in and added to the equipment for the acceptation in the workshop.14 MAINTENANCE15 AFTER-SALES SERVICES
RAL STAINER42 RAL STAINER User guideMessage Diagnosis SolutionWarning BBD expired Best Before Date expired Change the Staining Kit The activated carbon filter Carbon filter expired Change the activated carbonhas expired filter Tag error Bad tag position Check that bottle 1 is correctly placed as per the operating instructions in section 10.2.1SID tag incorrect The tag on your Change the staining kit Staining Kit is defective. or Contact your RAL Diagnostics representative.Motor unit card error Contact your RAL Instruments representative.Rinsing Station filling fault Check that the hydraulic circuit is not disconnected at some point. Turn off the appliance and then turn it back on. If the system is not restored, contact your RAL Instruments representative.Step loss on Theta Turn off the appliance and then Check that the arm is operatingCode 200 turn it back on correctly by simulating a Slide-holder incorrectly placed in staining kit change. the drawer If the system is not restored, Slide incorrectly placed on its support contact your RAL Instruments Drainage nozzle incorrectly placed representative. on its support16.1 ERROR MESSAGES16 TROUBLESHOOTINGStep loss on ZCode 1+tank number+1 = Loss of Z step when lowering to tank XExamples : Code 131 : Loss of Z step when lowering to tank 3Code 1A1 : Loss of Z step when lowering to rinsing tankCode 1B1 : Loss of Z step when picking up or dropping off basketCode 1+tank number+2 = Liquid missing in tank XExample :Code 142 : Liquid missing in tank 4Call a maintenance technicianThis can occur when the level detection could not be carried out.Check that the necks of the bottles are not dirtied.If so, clean them. Turn off the appliance and then turn it back on. Check that the arm is operating correctly by simulating a staining kit change. If the system is not restored, contact your RAL Instruments representative.Contact your RALInstruments representative.
RAL STAINER43 RAL STAINER User guide Problem encountered Diagnosis SolutionScreen off The green and blue diodes visiblewhen powering on on the USB be port are on?If yes Is the machine on standby? Press the standby button. ON/OFF too fast? Turn off the appliance and then turn it back on. If the system is not restored, contact your RAL Instruments representative.If not Contact your RAL Instruments representative.The machine does not turn on? Is the green diode that is visible inside the machine when the arm is in the soaking position in tank 3 on?If yes Contact your RAL Instruments representative.If not You must changed the power Contact your RAL Instruments supply or the fuse. representative.No reaction No beep when pressing Turn off the applianceof the touch screen impossible to enter the code. and turn it back on. If the system is not restored, contact your RAL Instruments representative.Incorrect reaction Contact your RAL Instrumentson the touch screen representative The codes do not allow Data loss Fill in the code to go to the codes page to initialise the machine. If the system is not restored, contact your RAL Instruments representative.The machine does not Memory problem There is an SD card in the RFIDkeep the codes in memory card located in the left reagent loading door. Check that the SD card is in the machine. If necessary, remove it and replace it after having cleaned the connections.Tag reading impossible Check whether the blue diode is on If the diode is on, try another"Tag reading" very long the RFID card and check on the tag. If it persists impossible, (greater than 10 seconds) info page whether the RFID software contact your RAL is present. Instruments representative. If the diode is off, contact your RAL Instruments representative.16.2 WHAT TO DO WHEN ...?
RAL STAINER44 RAL STAINER User guideProblem encountered Diagnosis SolutionStaining begins, but one of the positioning settings Contact yourslides are found is to be adjusted. RAL Instruments representative.on the machine plate.The slides are incorrectly Rinsing is carried out using Drain the rinsing container morerinsed soiled water. often or change the rinsing water in your container. If the fault persists there may be a fault on the draining pump. Contact your RAL Instruments representative.The rinsing tray overflows Contact your RAL Instruments representative.Variation of the soaking Check that there is no liquid Wipe the bottle necks.of the slides on the neck thread. If the fault persists, Contact your RAL Instruments representative to adjust the settings on the level sensor.The arm opens the lids The arm positions have become offset. Contact your RAL Instrumentseither too far or too little. representative.Major oscillation of the Too much space between the hooks Slightly tighten theslide holders during and the slide holders hooksZ movementsSystematic message Reagent levels low? Check the level of the liquid"Z step loss" in the containers. If necessary, if the arm is more than one year old, re-grease the Z axis. If the problem persists, contact your RAL Instruments representative.On installation the arm The machine has been taken out Wait 1 Hour moves a few mm, groans of its packing. The cold tightens the i.e. at room temperatureand stops moving the gaps and fixes the grease Use the "Load/Change the on the Z axis. staining kit" function several times to make the arm operate.The loading drawer Bad adjustment of the switch or Contact your RAL Instrumentsrefuses to shut electro-magnetic bearing. representative.Impossible to load Wrong USB stick Use the USB stick supplied withthe traceability programme your instrument or try to format your USB stick for FAT16. Other USB sticks are available on demand.
RAL STAINER45 RAL STAINER User guideAt all times it is possible to change users by clicking on the icon See section 8.2.3.A strict use of this tool makes it possible to enrich traceability related to the use of the machine.RAL Stainer permanently checks the level of the rinsing liquid and notifies you when the level becomes critical.RAL Stainer makes it possible to limit the volume of your effluents. You choose whether to drain the rinsing liquid after each dip. If you choose never to drain the rinsing liquid during a cycle, RAL Stainer will request the draining frequency by the question : "How many cycles before draining rising water?" Enter the value. If two stainings are launched, the arm will begin the second staining after having dropped the first slide holder back to its initial position. Before the second cycle begins, you can retrieve an awaiting slide holder to add or change a slide by pressing on the lock button.You can retrieve the first batch of slides after drying while the second batch is being stained.The slide holder can be retrieved while drying is in progress. 17 SUMMARY OF TIPS
RAL STAINER46 RAL STAINER User guide18 INDUSTRIAL PROPERTYThe RAL Stainer model is a community model registered under the n° 001733361-001.It is prohibited, unless explicitly authorised by RAL Instruments in writing, to manufacture, propose, put on the market, import, export, use or possess a product including the model for the same purposes.
RAL STAINER47 RAL STAINER User guide19 EC DECLARATION
RAL STAINER48 RAL STAINER User guide20 INDEXAActivated carbon filter .......................39CCleaning ...................................39DDecontamination ...........................40Description ................................11Draining ...................................34Drying ....................................34HHydraulic ..................................21IInstallation .................................19Interface ..................................22MMain settings ...............................33Maintenance ...............................42PPower cable ................................21Power supply ...............................21RRights management .........................28Rinsing ....................................34Rinsing solution ............................32SSettings page ...............................23Setting up ..................................20Slides .....................................35Smears .....................................9Staining ...................................36Staining reagents ............................16Standards ...................................9Symbols ....................................7TTraceability ................................38Troubleshooting ............................43UUser safety .................................10WWarnings ...................................9Warranty ...................................8Waste .....................................10
RAL INSTRUMENTS is a brand of RAL DIAGNOSTICSRAL DIAGNOSTICS - Site Montesquieu - 33650 Martillac - FRANCE E-mail: commercial@ral-diagnostics.fr Tel: +33 (0) 557 960 404 - Fax: +33 (0) 557 960 405 www.ral-instruments.com HAEMATOLOGYBACTERIOLOGYMYCO-PARASITOLOGYCYTOLOGYHISTOLOGYLeader in stains for microscopy since 1916

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