metraTec QR15HL RFID Module User Manual FM8 DOC022 52 53 90323

metraTec GmbH RFID Module FM8 DOC022 52 53 90323

user manual Dr3900

DOC022.53.90323DR 3900USER MANUAL05/2012 Edition 4© HACH-LANGE GmbH, 2011, 2012. All rights reserved. Printed in Germany.
3Table of ContentsSection 1 Specifications ........................................................................................................................ 9Section 2 General Information............................................................................................................. 112.1 Safety information............................................................................................................................. 112.1.1 Precautionary labels ................................................................................................................ 112.1.2 RFID module (not available on all models).............................................................................. 122.1.2.1 Certification..................................................................................................................... 122.1.3 Chemical and Biological Safety ............................................................................................... 142.2 Overview of product.......................................................................................................................... 14Section 3 Installation............................................................................................................................ 153.1 Unpack the instrument...................................................................................................................... 153.2 Operating environment ..................................................................................................................... 153.3 Front and back view.......................................................................................................................... 163.4 Power connections ........................................................................................................................... 173.5 Interfaces.......................................................................................................................................... 183.6 Cell compartments, cell adapters and light shield ............................................................................ 183.6.1 Cell compartments and adapters............................................................................................. 183.6.2 Installation of cell adapter ........................................................................................................ 203.6.3 Use of the light shield for measurements................................................................................. 213.7 Beam path ........................................................................................................................................ 22Section 4 Startup .................................................................................................................................. 234.1 Switch on the instrument, startup process........................................................................................ 234.2 Language selection........................................................................................................................... 234.3 Self-check......................................................................................................................................... 234.4 Sleep mode....................................................................................................................................... 244.5 Power off the instrument................................................................................................................... 24Section 5 Standard programs.............................................................................................................. 255.1 Overview........................................................................................................................................... 255.1.1 Tips for the use of the touch screen......................................................................................... 255.1.2 Use of the alphanumeric keypad ............................................................................................. 255.1.3 Main menu ............................................................................................................................... 26
4Table of Contents5.2 Instrument Setup...............................................................................................................................275.2.1 Operator ID...............................................................................................................................275.2.1.1 Operator RFID tag (not available on all models) .............................................................285.2.2 Sample ID.................................................................................................................................295.2.2.1 Sample ID with scanner method 1...................................................................................305.2.2.2 Sample ID with scanner method 2...................................................................................305.2.3 Sample ID and operator ID with the optional RFID Sample ID Kit (not available on all models) .315.2.4 Security settings.......................................................................................................................315.2.4.1 Assign operator security level..........................................................................................335.2.4.2 Deactivate password .......................................................................................................345.2.5 Date and time...........................................................................................................................355.2.6 Sound settings..........................................................................................................................365.2.7 PC and printer ..........................................................................................................................375.2.7.1 Printer setup ....................................................................................................................375.2.7.2 Print data .........................................................................................................................385.2.7.3 Print data continuously ....................................................................................................395.2.7.4 Network setup..................................................................................................................395.2.8 Power Management .................................................................................................................425.3 Save, recall, send and delete data....................................................................................................435.3.1 The data log .............................................................................................................................435.3.1.1 Auto/manual data storage ...............................................................................................435.3.1.2 Recall stored data from the data log................................................................................445.3.1.3 Send data from the data log ............................................................................................455.3.1.4 Delete stored data from the data log ...............................................................................465.3.2 Control charts for data from the AQA Log................................................................................465.3.3 Store, recall, send and delete data from wavelength scan and time course............................485.3.3.1 Data storage from wavelength scan or time course........................................................485.3.3.2 Recall stored data from wavelength scan or time course................................................495.3.3.3 Send data from wavelength scan or time course ............................................................495.3.3.4 Delete stored data from wavelength scan or time course ...............................................505.3.4 Data analysis............................................................................................................................515.3.4.1 Trends .............................................................................................................................515.3.4.2 Ratios ..............................................................................................................................535.3.4.3 Interference Check (not available on all models) ............................................................56
5Table of Contents5.4 Stored Programs............................................................................................................................... 575.4.1 Select a saved test/method; entering user-specific basic data................................................ 575.4.2 Stored programs options.......................................................................................................... 575.4.3 Use of program timers ............................................................................................................. 595.4.4 Set the dilution factor ............................................................................................................... 595.4.5 Run a standard adjust.............................................................................................................. 605.4.6 Set the chemical form .............................................................................................................. 605.4.6.1 Change of the default setting of the chemical form......................................................... 615.4.7 Run a reagent blank................................................................................................................. 615.4.8 To carry out a multiple determination....................................................................................... 625.4.9 Analysis of samples ................................................................................................................. 625.4.10 Update/edit tests (not available on all models) ...................................................................... 635.4.10.1 General advices for program update and edit............................................................... 635.4.10.2 Manual update of test data ........................................................................................... 635.4.10.3 Program a new test....................................................................................................... 645.4.11 Add stored programs to the favorite programs list................................................................. 645.5 Barcode Programs............................................................................................................................ 655.5.1 Complete a barcode test.......................................................................................................... 655.5.2 Select the chemical evaluation form ........................................................................................ 665.5.2.1 Change of the default setting of the chemical form......................................................... 665.5.3 Basic test-specific and sample-specific data settings.............................................................. 665.5.4 Sample blank ........................................................................................................................... 685.5.5 Update/edit barcode tests........................................................................................................ 685.5.5.1 Update barcode tests with test packaging (not available on all models) ........................ 685.5.5.2 Manual update of a barcode test .................................................................................... 695.5.5.3 Manual check/revision of test data.................................................................................. 715.5.5.4 Update via the Internet.................................................................................................... 715.5.5.5 Program a new test......................................................................................................... 72
6Table of ContentsSection 6 Advanced operations...........................................................................................................736.1 User programs...................................................................................................................................736.1.1 Program a user method............................................................................................................746.1.1.1 Single wavelength settings..............................................................................................756.1.1.2 Multi wavelength settings ................................................................................................766.1.1.3 Calibration settings for single and Multi Wavelength mode.............................................786.1.1.4 Store a user program.......................................................................................................816.1.1.5 Additional user-defined parameters and functions ..........................................................816.1.2 "Free programming" program type...........................................................................................826.1.2.1 Measurement process.....................................................................................................846.1.2.2 Enter a new element of a measuring sequence ..............................................................846.1.2.3 Enter the calibration formula (evaluation formula)...........................................................876.1.2.4 Enter variables.................................................................................................................906.1.2.5 Save a free programming user program..........................................................................906.1.3 Select a user program..............................................................................................................906.1.4 Add, edit and delete user programs from the favorites list.......................................................906.1.4.1 Add to favourites..............................................................................................................916.1.4.2 Edit ..................................................................................................................................916.1.4.3 Clear Entry.......................................................................................................................926.2 Favorites ...........................................................................................................................................926.2.1 Recall a favorite program .........................................................................................................926.2.2 Delete a favorite program.........................................................................................................926.3 Standard Addition - monitoring/checking results...............................................................................936.3.1 Complete a standard addition ..................................................................................................946.4 Single Wavelength (absorbance, concentration and transmittance readings) ..................................976.4.1 Set up Single Wavelength mode ..............................................................................................976.4.2 Take single wavelength readings (single reading) ...................................................................996.5 Multi Wavelength mode – readings with more than one wavelength ................................................996.5.1 Set the Reading mode at different wavelengths.......................................................................996.5.2 Complete a reading in the Multi Wavelength mode................................................................1026.6 Wavelength Scan mode – recording of absorbance and transmission spectrums..........................1026.6.1 Set up the wavelength scan ...................................................................................................1036.6.2 Wavelength scan reading.......................................................................................................1056.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis.....................1066.6.3 Work with reference scans .....................................................................................................1066.7 Time course of absorbance/transmittance......................................................................................1086.7.1 Time course setup parameters...............................................................................................1086.7.2 Time course scan reading ......................................................................................................1096.7.3 Analysis of time course data ..................................................................................................1106.7.3.1 Navigation of a time scan or a time scan analysis.........................................................110
7Table of Contents6.8 System Checks............................................................................................................................... 1116.8.1 Instrument Information........................................................................................................... 1116.8.2 Update the instrument software............................................................................................. 1116.8.3 Optical Checks....................................................................................................................... 1126.8.3.1 Verification kit................................................................................................................ 1126.8.3.2 Test solution.................................................................................................................. 1146.8.3.3 Wavelength check......................................................................................................... 1156.8.3.4 Stray light check............................................................................................................ 1166.8.3.5 Absorbance check ........................................................................................................1166.8.4 AQA - Analytical Quality Assurance....................................................................................... 1176.8.4.1 AQA Standard Configuration ........................................................................................ 1186.8.4.2 AQA Addition ................................................................................................................ 1196.8.4.3 Multiple Determination ..................................................................................................1206.8.4.4 Dilution.......................................................................................................................... 1216.8.4.5 Carring out AQA measures........................................................................................... 1216.8.4.6 Pipette test set .............................................................................................................. 1216.8.5 Instrument backup ................................................................................................................. 1226.8.6 Service menu......................................................................................................................... 1236.8.7 Service time ........................................................................................................................... 1236.8.8 Lamps operating time ............................................................................................................ 1246.9 Toolbar............................................................................................................................................ 1246.9.1 Log on.................................................................................................................................... 1246.9.2 Sample ID .............................................................................................................................. 1256.9.3 Timer...................................................................................................................................... 1256.9.4 AQA ....................................................................................................................................... 1256.9.5 Trends.................................................................................................................................... 1266.9.6 Link2SC ................................................................................................................................. 1266.9.7 Website DR 3900................................................................................................................... 126Section 7 Maintenance....................................................................................................................... 1277.1 Cleaning requirements.................................................................................................................... 1277.1.1 Spectrophotometer ................................................................................................................ 1277.1.2 Display ................................................................................................................................... 1277.1.3 Cuvettes/cells......................................................................................................................... 1287.2 Lamp replacement.......................................................................................................................... 1287.3 Exchange of the replaceable cell compartment.............................................................................. 131Section 8 Troubleshooting................................................................................................................. 133Section 9 Replacement Parts............................................................................................................. 1379.1 Accessories .................................................................................................................................... 1379.2 Replacement Parts ......................................................................................................................... 138
8Table of ContentsSection 10 Contact .............................................................................................................................139Section 11 Warranty, liability and complaintsLimited warranty......................................................141Appendix A Help Guide ......................................................................................................................143A.1 Display of the Help Guide for stored programs..............................................................................143A.2 Display of the Help Guide for barcode programs..........................................................................144Index.....................................................................................................................................................147
9Section 1 SpecificationsThese are subject to change without notice!Performance specificationsOperating mode Transmittance (%), Absorbance and ConcentrationSource lamp Halogen lamp Wavelength range 320–1100 nmWavelength Accuracy ± 1.5 nm (wavelength range 340–900 nm)Wavelength reproducibility ± 0.1 nmWavelength resolution 1 nmWavelength calibration AutomaticWavelength selection Automatic, based on method selectionScanning speed  8 nm/s (in steps of 1 nm)Spectral bandwidth 5 nmPhotometric measuring range ± 3.0 Abs (wavelength range 340–900 nm)Photometric accuracy 5 m Abs at 0.0–0.5 Abs1% at 0.50–2.0 AbsPhotometric linearity < 0.5% to 2 Abs1% at > 2 Abs with neutral glass at 546 nm Stray light < 0.1% T at 340 nm with NaNO2Data log 2000 readings (Result, Date, Time, Sample ID, User ID)User programs 100Physical and environmental specificationsWidth 350 mm (13.78 in)Height 151 mm (5.94 in)Depth 255 mm (10.04 in)Mass 4200 g (9.26 lb)Ambient operating requirements 10–40 °C (50–104 °F), maximum 80% relative humidity (without condensate formation)Ambient storage requirements –40–60 °C (–40–140 °F), maximum 80% relative humidity (without condensate formation)Additional technical dataPower connector via external power supply Input:100–240 V/50–60 HzOutput: 15 V/30 VAInterfacesUse only shielded cable with maximum length of 3 m:2x USB type A1x USB type BUse only shielded cable (e.g. STP, FTP, S/FTP) with maximum length of 20 m:1x Ethernet
10SpecificationsEnclosure rating IP40 (excluding interfaces and power supply)Protection class Class IThis product has been tested to the requirements of CAN/CSA-C22.2 No. 61010-1, second edition, including Amendment 1, or a later version of the same standard that incorporates the same level of testing requirements.Performance specifications
11Section 2 General Information2.1 Safety informationPlease read this entire manual before unpacking, setting up, or operating this equipment. Pay attention to all danger and caution statements. Failure to do so could result in serious injury to the operator or damage to the equipment. To make sure that the protection provided by this instrument is not impaired, do not use or install this instrument in any manner other than that specified in these operating instructions.Note: Information that supplements points in the main text.2.1.1 Precautionary labelsRead all labels and tags attached to the instrument. Personal injury or damage to the instrument could occur if this information is not observed. A symbol, if noted on the instrument, will be included with a danger or caution statement in the operating instructions. DANGERIndicates a potentially or imminently hazardous situation that, if not avoided, results in death or serious injury.WARNINGIndicates a potentially or imminently hazardous situation that, if not avoided, may result in death or serious injury.CAUTIONIndicates a potentially hazardous situation that may result in minor or moderate injury.NOTICEIndicates a situation that, if not avoided, could result in damage to the instrument. Information that requires special emphasis. This symbol, if noted on the instrument, references the user manual for operation and/or safety information.Electrical equipment marked with this symbol may not be disposed of in European domestic or public disposal systems after 12 August 2005. In conformity with European local and national regulations (EU Directive 2002/96/EC), European electrical equipment users must now return old or end-of life equipment to the manufacturer for disposal at no charge to the user. Note: For return for recycling, please contact the equipment manufacturer or supplier for instructions on how to return end-of-life equipment, manufacturer-supplied electrical accessories, and all auxiliary items for correct disposal.
12General Information2.1.2 RFID module (not available on all models)RFID technology is a radio application. Radio applications are subject to national conditions of authorisation. The use of the DR 3900 (model with RFID module) is currently permitted in the following countries: EU, CH, NO, HR, RS, MK, TR, CY, US, CA, AU, NZ. The manufacturer advises that the use of the DR 3900 (model with RFID module) outside of the above-mentioned regions may contravene national laws. The manufacturer reserves the right also to obtain authorisation in other countries. In case of doubt, please contact the distributor.The DR 3900 contains an RFID module to receive and transmit information and data. The RFID module operates with a frequency of 13.56 MHz.Follow the following safety information, in addition to any local guidelines in force.Safety information for the correct use of the instrument:•Do not operate the instrument in hospitals or comparable establishments in proximity to medical equipment, like pace makers or hearing aids.•Do not operate the instrument near highly flammable substances, such as fuels, highly combustible chemicals and explosives.•Do not operate the instrument near combustible gases, vapors or dust.•Do not vibrate or jolt the instrument.•The instrument can cause interference in immediate proximity to televisions, radios and computers.•Do not open the instrument.•Improper use of the instrument voids the warrantee. 2.1.2.1 Certification (integrated RFID module)FCC ID: YUH-QR15HLIC: 9278A-QR15HLThis device complies with Part 15 of the FCC rules and Industry Canada license exempt RSS standard(s).Operation is subject to the following two conditions:1. this device may not cause interference, and2. this device must accept any interference, including interference that may cause undesired operation of the device.Le présent appareil est conforme aux CNR d'Industrie Canada applicables aux appareils radio exempts de licence.WARNINGThe spectrophotometer may not be used in dangerous environments.The manufacturer and its suppliers reject any express or indirect guarantee for the use with High Risk Activities.
13General InformationL'exploitation est autorisée aux deux conditions suivantes :1. l'appareil nedoit pas produire de brouillage, et2. l'utilisateur de l'appareil doit accepter tout brouillage radioélectriquesubi, même si le brouillage est susceptible d'en compromettre le fonctionnement.Changes or modifications to this equipment not expressly approved by the party responsible for compliance could void the user's authority to operate the equipment.This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference, in which case the user will be required to correct the interference at their expense. The following techniques can be used to reduce interference problems:1. Disconnect the equipment from its power source to verify that it is or is not the source of the interference.2. If the equipment is connected to the same outlet as the device experiencing interference, connect the equipment to a different outlet.3. Move the equipment away from the device receiving the interference.4. Reposition the receiving antenna for the device receiving the interference.5. Try combinations of the above.
14General Information2.1.3 Chemical and Biological SafetyNormal operation of this instrument may involve the use of hazardous chemicals or biologically harmful samples.•Observe all cautionary information printed on the original solution containers and safety data sheet prior to their use.•Dispose of all consumed solutions in accordance with national regulations and laws.•Select the type of protective equipment suitable to the concentration and quantity of the dangerous material at the respective work place.2.2 Overview of productThe DR 3900 is a VIS spectrophotometer with a wavelength range of 320 to 1100 nm. The instrument is delivered with a complete series of application programs and supports several languages. The spectrophotometer DR 3900 contains the following programs and operating types: •Stored programs (pre-installed tests)•Barcode Programs•User Programs•Favorites•Single Wavelength•Multi Wavelength•Wavelength Scan•Time courseThe DR 3900 Spectrophotometer provides digital readouts in direct concentration units, absorbance or percent transmittance.When selecting a user-generated or programmed method, the menus and prompts serve to direct the user through the test.This menu system can also generate reports, statistical evaluations of generated calibration curves and to report instrument diagnostic checks.DANGERPotential danger in the event of contact with chemical/biological materials.Handling chemical samples, standards and reagents can be dangerous. Familiarise yourself with the necessary safety procedures and the correct handling of the chemicals before the work and read and follow all relevant safety data sheets.
15Section 3Installation3.1 Unpack the instrumentThe DR 3900 Spectrophotometer comes packaged with the following items:•DR 3900 spectrophotometer•Dust Cover•USB dust cover, fitted as standard•Benchtop power supply with power cord for US, GB•Cell adapter A•Light shield, fitted as standard in the DR 3900•Operator RFID tag (not available on all models)•Basic operating instructions DR 3900, operating instructions LINK2SC•CD-ROM with HACH and HACH-LANGE procedural instructions and extensive operating instructionsNote: If any of these items are missing or damaged, please contact the manufacturer or a sales representative immediately.3.2 Operating environmentNote the following points so that the instrument will operate perfectly and have a long service life. •Place the instrument firmly on an even surface. Do not push any objects under the instrument.•The ambient temperature must be 10–40 °C (50–104 °F).•The relative humidity should be less than 80%; moisture should not condense on the instrument.•Leave at least a 15 cm clearance at the top and on all sides for air circulation, to avoid overheating of electrical parts.•Do not operate or store the instrument in extremely dusty, humid or wet locations.•Keep the surface of the instrument, the cell compartment and all accessories clean and dry at all times. Immediately remove splashes or spilt materials on or in the instrument (refer to Section 7).WARNINGElectrical and fire hazards. Only use the supplied benchtop power supply LZV844. Only qualified experts may perform the tasks described in this section of the manual, while adhering to all locally valid safety regulations.NOTICEProtect the instrument from extreme temperatures from heaters, direct sunlight and other heat sources.
16Installation3.3 Front and back viewFigure 1 Front view 1USB port type A 4On/off switch2Cell compartment cover 5RFID module (not available on all models)3Touch screen
17InstallationFigure 2 Back view 3.4 Power connections1. Connect the power cable to the benchtop power supply. 2. Plug the benchtop power supply cable into the back of the instrument (Figure 2, page 17).3. Insert the power cable plug into a mains socket (100–240 V~/50–60 Hz). 4. Turn on the power button next to the display to turn on the power supply (Figure 1, page 16).1Ethernet port 3USB port type A2USB port type B 4Connection for benchtop power supplyWARNINGElectrical and fire hazards. Only use the supplied benchtop power supply LZV844.
18Installation3.5 InterfacesThe DR 3900 features three USB ports and one Ethernet port as standard, located on the front and rear sides of the instrument (Figure 1 and Figure 2, page 17). The USB type A ports are used for communications with a printer, USB memory stick or keyboard. A USB memory stick can be used to update the instrument software.The USB type B port is used for communications with a PC. The optional Hach Data Trans software (refer to Section 9, page 137) must be installed on the PC for data transfer.A USB hub may be used to connect several accessories at a time.Note: USB cables must not be longer than 3 m.These USB ports enable data and graphics to be output to a printer or PC and they enable instrument software to be upgraded (refer to Section 6.8.2, Page 111). The Ethernet port supports real-time data transfer in local networks, LIMS systems or SC controllers. Only use a shielded cable (e.g. STP, FTP, S/FTP) with a maximum length of 20 m for the Ethernet port.3.6 Cell compartments, cell adapters and light shield3.6.1 Cell compartments and adaptersThe DR 3900 has two cell compartments (Figure 3). Only one cell type at a time can be used for a reading.Cell compartment (1) for:•13-mm and 16-mm round cellsNote: Cell compartment (1) contains barcode detection for cells/vials.Cell compartment (2) for:The following cell types can be used in cell compartment (2).•Without cell adapter A, 50-mm cells, 1-inch rectangular cells and 1-inch flow cells can be used directly in cell compartment (2). •With cell adapter A: 10 mm rectangular cells, 1-inch round cells and AccuVac® vials.Note: These cells must be inserted using cell adapter A.
19InstallationFigure 3 Cell compartments Figure 4 Cell adapter 1Cell compartment for round cells (1) 2Cell compartment for rectangular cells (2)Cell adapter A: 10-mm rectangular cells/1-inch round cell
20Installation3.6.2 Installation of cell adapter1. Open the cell compartment.2. Place the cell adapter into cell compartment (2) so that the arrow on the cell adapter points to the rear (Figure 5). Note: The arrow on top of the cell adapter indicates the direction of the light beam path.Figure 5 Installation of a cell adapter
21Installation3.6.3 Use of the light shield for measurementsThe light shield (Figure 6) prevents disruptive exposure to light when 13-mm or 16-mm round cells are used in the cell compartment (1).Insert the light shield into cell compartment (2) before performing measurements in cell compartment (1).The DR 3900 is shipped with the light shield installed. Remove the light shield before using cell compartment (2). Figure 6 Light shield Installation of the light shield1. Open the cell compartment.2. Place the light shield into cell compartment (2) so that the arrow on the light shield points to the rear.
22Installation3.7 Beam pathFigure 7 shows the beam path of the DR 3900.Figure 7 Beam path 1Halogen lamp 8Splitter mirror2Entrance slit 9Reference element3Grating 10 Cell compartment (2) for rectangular cells4Grating angle indicator 11 Lens5Exit slit 12 Reading element6Lens 13 Cell compartment (1) for round cells7Filter wheel
23Section 4Startup4.1 Switch on the instrument, startup process1. Connect the power cable to the mains outlet.2. Switch on the instrument by using the power button next to the display.3. The instrument starts automatically with a startup process lasting approximately 45 seconds. The display shows the logo of the manufacturer. At the end of the startup process, a startup melody is heard.Note: Wait approximately 20 seconds before switching on again so as not to damage the electronics and mechanics of the instrument.4.2 Language selectionThe DR 3900 software includes several language options. The first time the instrument is switched on, the language selection screen will be shown automatically after the startup process.1. Select the required language.2. Press OK to confirm the language selection. The self-check will then start automatically.Change the language settingThe instrument functions in the selected language until the option is changed.1. Turn the instrument on.2. During the startup process, touch any point on the screen and maintain contact until the option for selecting a language is shown (approximately 45 seconds).3. Select the required language.4. Press OK to confirm the language selection. The self-check will then start automatically.4.3 Self-checkEach time the instrument is powered up, a test program begins.This procedure, which takes approximately two minutes, checks the system, lamp, filter adjustment, wavelength calibration and voltage. Each test that functions correctly is marked accordingly.The Main Menu is displayed when diagnostics are completed.Note: In the event of further error messages during the test program, refer to Section 8, page 133.
24Startup4.4 Sleep modeThe instrument can be put into sleep mode. 1. Briefly press the power button next to the display.The "Sleep mode" message is shown. The display will then switch off automatically.2. To switch on, press the power button next to the display.The self-check will start automatically. After that, the instrument is ready to use.4.5 Power off the instrument1. Press the power button next to the display for approximately 5 seconds.
25Section 5 Standard programs5.1 Overview5.1.1 Tips for the use of the touch screenThe whole screen responds to touch. To choose an option, tap with a fingernail, fingertip, an eraser or a specialised stylus. Do not press the screen with a sharp object, such as the tip of a ballpoint pen.•Do not place anything on top of the screen, to prevent damage or scratches on the screen.•Press buttons, words or icons to select them. •Use scroll bars to move up and down long lists very quickly. Press and hold the scroll bar, then move up or down to move through the list. •Highlight an item from a list by pressing it once. When the item has been successfully selected, it will be displayed as reversed text (light text on a dark background).5.1.2 Use of the alphanumeric keypadThis display is used to enter letters, numbers and symbols as needed when programming the instrument. Unavailable options are disabled (grayed out). The icons on the right and left of the screen are described in Table 1.The central keypad changes to reflect the chosen entry mode. Press a key repeatedly until the desired character appears on the screen. A space can be entered by using the underscore on the YZ_ key.Press Cancel to cancel an entry, or press OK to confirm an entry.Note: It is also possible to use a USB keyboard (with US keyboard layout) or a hand-held USB barcode scanner (refer to Section 9, page 137).Table 1 Alphanumeric keypadIcon / key Description FunctionABC/abc Alphabetic Toggles the character input mode between upper and lower case.# % Symbols Punctuation, symbols and numerical sub- and superscripts may be entered.123 Numeric For entering regular numbers.CE Clear Entry Clear the entry.Left Arrow Back Deletes the current character and goes back one position.Right Arrow Next Navigates to the next space in an entry.
26Standard programs5.1.3 Main menuA variety of modes may be selected from the Main Menu. The following table briefly describes each menu option.There is a toolbar on the right-hand side of the screen. Press to activate the various functions.Table 2 Main Menu optionsOption FunctionStored Programs / Barcode Programs(HACH-LANGE programs)Stored programs are pre-programmed methods that make use of HACH chemicals and HACH-LANGE pipette tests.The DR 3900 Procedures Manual contains illustrated, step-by-step procedures for analyses using HACH programs. The working procedures for HACH-LANGE tests are included in the test packs.User ProgramsUser programs make "made to measure analysis" possible:•Users can program methods they have developed themselves•Existing HACH and LANGE methods can be stored as user programs. The HACH-LANGE tests can then be modified to suit the user's requirements.Favorites List of methods/tests created by the user to suit his own requirements.Single WavelengthSingle wavelength readings are:Absorbance readings: The light absorbed by the sample is measured in absorbance units. Transmittance reading (%): Measures the percent of the original light that passes through the sample and reaches the detector.Concentration readings: A concentration factor can be entered to enable the measured absorbance values to be converted into concentration values.Multi Wavelength In the Multi Wavelength mode, absorbance (Abs) or percentage transmittance (%T) is measured at up to four wavelengths and absorbance differences and absorbance relationships are calculated. Simple conversions into concentrations can also be carried out.Wavelength ScanA wavelength scan shows how the light from a sample is absorbed over a defined wavelength spectrum. This function can be used to determine the wavelength at which the maximum absorbance value can be measured. The absorbance behavior is displayed graphically during the scan.Time course The time scan records the absorbance or % transmittance at a wavelength over a defined time.System checks The "System check" menu offers a number of options, including optical checks, output checks, lamp history, instrument update, service time, settings for analytical quality assurance and instrument backup.Recall measurement data Stored data can be called up, filtered, sent and deleted.Instrument Setup In this mode, user-specific or method-specific settings can be entered: operator ID, sample ID, date & time, sound, PC & printer, password, energy-saving mode and stored data.
27Standard programs5.2 Instrument Setup1. Select Instrument Setup in the Main Menu.A selection of functions appears in order to configure the functions of the instrument.5.2.1 Operator IDUse this option to enter up to 30 sets of operator initials (up to ten characters each) into the instrument. This feature helps record which operator measured each sample.1. Press Operator ID in "Instrument Setup".2. Press Options>New to enter a new operator ID. Note: If no operator ID has yet been entered, the alphanumeric keyboard is immediately displayed.3. Use the alphanumeric keypad to enter a new operator ID.4. Press OK to confirm the entry.5. Choose an icon for the operator ID using the Left Arrow and Right Arrow.6. Press Operator Password to protect the operator ID with a password.7. Use the alphanumeric keyboard to enter an operator password.8. Press OK to confirm the entry.9. Press Cancel to delete the complete entry for the operator ID. Press Back to reach to the input screen for operator ID. Press OK to confirm the entry. The following screen is displayed.
28Standard programs10. Press Back to return the instrument to the "Instrument Setup" menu.Press Logout to log out of an active operator ID.Press Login to activate a selected operator ID.Press Options to enter, change or delete additional operator IDs. The following screen is displayed.11. Press New to enter another operator ID.Press Delete to delete an operator ID.Press Edit to edit an operator ID.Press Initialize RFID Tag to write the operator ID to an optional operator RFID tag (not available on all models). Refer to Section 5.2.1.1 for more information.5.2.1.1 Operator RFID tag (not available on all models) 1. Define an operator ID (refer to Section 5.2.1).2. Press Initialize RFID Tag. 3. Follow the instructions on screen and hold the operator RFID tag over to the front-right of the RFID module.A sound indicates that the write process has been sucessful.4. The operator RFID tag can be newly written at any time. To do so, confirm the "Overwrite Operator ID" message with OK and enter the password if the old operator ID is protected with a password.If the operator RFID tag is successfully overwritten, a message confirming this will briefly be shown on the display.The operator is logged on and the operator ID is shown in the toolbar.
29Standard programs5.2.2 Sample IDUse this option to enter up to 100 Sample Identification tags (up to 20 characters each) into the instrument. Sample IDs can be used to specify the sample location or other sample specific information.1. Press Sample ID in the toolbar on the right.2. Press New to enter a new sample ID.Note: If no sample ID has been entered yet, the alphanumeric keyboard is immediately displayed.3. Use the alphanumeric keypad to enter a new Sample ID.Note: If a USB Barcode handset scanner ( refer to Section 5.2.2.1, page 30) is connected, Sample IDs can also be scanned.4. Press OK to confirm the entry.5. Assign the current time and date, a sequential number or a colour to the sample ID.6. To number the sample IDs sequentially—e.g. Inflow (01) etc.—press Add Number. •Use the arrow keys to specify the first number of the sequence.•Use the key between the arrow keys to enter the first number of the sequence using the alphanumeric keypad.7. Press OK to return to the "Instrument Setup" menu. The Sample ID is activated. Each Sample ID is automatically numbered in ascending order after a reading. The number is shown in parentheses behind the Sample ID.8. Press Back to return the instrument to the "Instrument Setup" menu.Press Off to switch off the active sample ID.Press Select to activate the selected sample ID.Use Options to enter, change or delete additional sample IDs. The following screen is displayed:
30Standard programs9. Press New to enter another sample ID.Press Delete to delete a sample ID.Press Edit to edit a sample ID.Press Initialize RFID Tag to write the sample ID to an optional location RFID tag (not available on all models). 5.2.2.1 Sample ID with scanner method 11. Connect the scanner to the USB port. Successful connection is indicated by a sound.2. Press Sample ID>Options and New.3. Read barcode with scanner.4. The sample ID can have the current date and time, a sequential number and a colour allocated to it.Select the required options or a colour. 5. Press OK to confirm the entry.6. Press New again and repeat the process for each barcode.5.2.2.2 Sample ID with scanner method 21. Read the sample ID in the measurement window with the barcode scanner and measure the cell.The barcode is stored along with the reading, but is not adopted into the list of sample IDs.Note: To delete a sample ID, select the ID and press Delete.Note: A sample ID can be entered or changed in Reading Mode. On the results screen, press Options>More>Instrument Setup. If a Sample ID is already assigned, select the "Sample ID" symbol in the result display.
31Standard programs5.2.3 Sample ID and operator ID with the optional RFID Sample ID Kit (not available on all models)The optional RFID Sample ID Kit consists of: •A LOC 100 RFID Locator•An operator RFID tag•Five location RFID tags and •Three sample bottle RFID labels each, in black, red, green, blue and yellowThe operator and location RFID tags can be written to and read in by the RFID module in the DR 3900 as often as required.More information can be found in the user manual for the LOC 100 RFID Locator.5.2.4 Security settingsThe "Security" menu contains a variety of security settings to control access to various functions. All functions can be allocated to three different security levels:• not secured: every operator can make changes in this area.• one key: every operator assigned this security level can make changes to non-secured functions and functions with one key.• two keys: every operator assigned this security level can make changes in all functions1. Press Security in the "Instrument Setup" menu.2. In order to open the Security List, assign a password as security administrator. Press Security Password.3. Use the alphanumeric keypad to enter a new Password (up to 10 characters each) and press OK to confirm.
32Standard programs4. Press Security List to use various functions with security level 1 or 2.5. Select the required function and press Setup.6. Select the required security level (two keys, one keys or off) and confirm using OK. 7. Confirm the Security List using OK to return to the "Security" menu.8. Press On to activate the new settings of the security list.9. Press OK to return to the "Instrument Setup" menu.Note: The alphanumeric keypad for entering the password appears when a user tries to reach a locked setting.
33Standard programs5.2.4.1 Assign operator security levelEvery operator with an operator ID can be assigned a security level. This is connected to the operator password. Setup must be coordinated with the security administrator and the operator.1. Press Operator ID in the "Instrument Setup".2. Enter the security password and confirm by pressing OK.3. Set up an operator ID (refer to Section 5.2.1).4. Press Operator Password.5. Enter the operator password and confirm with OK.6. Press Security Level <Off>7. Enter the security password and confirm with OK.The current security level for the selected operator is shown.8. Select the required security level for this operator ID and confirm with OK.
34Standard programs9. Press OK.The operator ID is displayed with the selected security level. 10. Activate the selected operator ID by pressing Login.11. Enter the operator password. 12. Confirm with OK and return to "Instrument Setup". 5.2.4.2 Deactivate password1. Press Security in the "Instrument Setup" menu.2. Enter the security password and confirm with OK.3. Press Off to deactivate the settings of the Security List.4. Press OK and return to the "Instrument Setup" menu.Note: Use this function to delete the former password or to enter a new one.
35Standard programs5.2.5 Date and time1. Press Date & Time in the "Instrument Setup".2. Select a Date Format for the date.3. Press OK to confirm the entry.4. Select a Time Format for the time.5. Press OK to confirm the entry.6. Press OK to confirm the entry.
36Standard programs7. Enter the current date and time. Change the information using the arrow keys. 8. Press OK to confirm the entry. The instrument will return to "Instrument Setup".5.2.6 Sound settings 1. Press Sound Settings in "Instrument Setup".The following options will be displayed:• All: Activates/deactivates a sound with a variable volume for every function, with the exception of the timer.• Touch screen: Activates/deactivates a short sound with variable volume every time the screen is touched.• Reading done: Activates/deactivates a short sound with variable volume when the reading is done.•Timer: Sets short/long sound with variable volume when timer has ended.•Startup: Activates/deactivates a startup melody with variable volume when the test program is started.• Warning: Activates/deactivates a short sound with variable volume if there is an error message.• RFID done: Activates/deactivates a short sound with variable volume when a RFID transfer is done.• Shutdown: Activates/deactivates a short sound with variable volume when shutting down.2. Select the required action.3. Press Setup and complete the required settings.4. Press OK to confirm the entry.5. Press OK to confirm the entry. The instrument then returns to "Instrument Setup".
37Standard programs5.2.7 PC and printerThe DR 3900 features an Ethernet port and two USB ports on the rear of the instrument, as well as one USB port on the front (refer to Figure 1 and Figure 2, page 17). These interfaces can be used for exporting data and graphics to a printer, updating data and transferring data to a PC and in a network. These interfaces can be used for the connection of a USB stick, an external USB keyboard or a USB Barcode handset scanner. Note: Various accessories can also be connected simultaneously using a USB hub.A USB memory stick is used to update data, refer to Section 6.8.2, page 111.Note: A shielded USB cable must not be longer than 3 m!1. Press PC & Printer in "Instrument Setup".A list with information about the connections opens.5.2.7.1 Printer setup2. Press Printer.3. Press Setup to display the Printer Setup screen.Table 3 USB connectorInterfaces DescriptionUSB (Type A) This USB port can be used to connect a printer, a USB memory stick or a keyboard. USB (Type B) This USB port is only intended for the connection of the DR 3900 to a PC (with installation of the corresponding software).Ethernet The Ethernet port is intended for data transfer to a PC without installed software or in a local network . Only use a shielded cable (e.g. STP, FTP, S/FTP) with a maximum length of 20 m for the Ethernet port.
38Standard programsPrinter Setup: •Resolution: Font size•Paper: Paper sizeNote: If an optional Thermal Printer is connected, the function "Auto Send" on/off is available.4. Press Resolution to select the print quality. Select between:•100 dpi,•150 dpi and•300 dpi.5. Press OK to confirm the entry.Note: Press OK again to return to the "Instrument Setup" menu.6. Press Paper to select the paper size.Select between:•Letter,•Monarch,•Executive,•A4.7. Press OK to confirm the entry.Note: Press OK again to return to the "Instrument Setup" menu.5.2.7.2 Print data1. Press Recall Data from the Main Menu.2. Select the data source, where the data to be printed are stored.A list is displayed. Data can be filtered. Refer to Section 5.3.1.2, page 44 for more information.3. Press the Printer icon to send the data (table, curve) immediately to the printer.4. Highlight Single point, Filtered data or All data and press OK to confirm.Send data... appears on the display until the data is printed.
39Standard programs5.2.7.3 Print data continuouslyIf all readings are to be printed out automatically straight after measurement, the use of a continuous form printer is recommended, e.g. the 4" paper thermal printer. Refer to Section 9, page 137.1. Connect the thermal printer to a USB type A port.2. Press PC & Printer in the "Instrument Setup" menu.A list with information about the connections opens.3. Highlight Printer.4. Press Setup to display the Printer Setup screen.5. Select Auto-Send: On to send all measured data automatically to the Thermal printer.5.2.7.4 Network setupThe DR 3900 supports communication in local networks. To establish Ethernet communication in networks, special configuration is required. The manufacturer recommends support from a network administrator. Only use a shielded cable (e.g. STP, FTP, S/FTP) with a maximum length of 20 m for the Ethernet port. This section describes the setting options for the photometer in relation to network communication. The connection parameters must be configured for data transfer to a PC or connection to a network.1. Press PC & Printer in "Instrument Setup".2. Press Network>Setup.3. Press On.4. Press IP address.An IP address is used to send data from a sender to a pre-defined recipient.•If the IP for the DR 3900 is automatically issued in the network, no further settings are necessary. A hostname can optionally be assigned.•If the instrument address is fixed, the connection must be configured.
40Standard programs5. Select Fix to configure the IP address.6. Press IP address and enter the address.In conjunction with the IP address of an instrument, the subnet mask establishes which IP addresses are located within the local network.7. Press Subnet Mask and enter the address.A Gateway enables data communication in networks that are based on varying protocols.8. Press Default Gateway and enter the address.9. Press OK to confirm the entry.
41Standard programs10. Press Network Server: <Off> to determine the target site for the data transfer.11. Select Netdrive and run Netdrive Setup.Alternatively, select FTP and run FTP Setup.The procedures are completely identical. As an example, Netdrive Setup is described here. The target site can be defined by IP address or a server name. 12. Press IP-Address and enter the IP address or select Server Name and enter the server name.13. Press Data Folder and enter the target folder name.Note: A folder with the selected name must exist on the target system. Additionally, the folder must be shared with the operator, using the corresponding password.14. Press OK to confirm the entry.
42Standard programsUser and password must be identical to the settings on the target system.15. Press User and enter the username.16. Press OK to confirm.17. Press Password and enter the password.18. Press OK to confirm the entry.19. Press Factory Default to restore the Network Settings to the factory defaults.5.2.8 Power Management1. Press Power Management in "Instrument Setup".2. Select Sleep Timer and press OK.3. Select the required time span after which the instrument will go into energy-saving sleep mode if it is not used (refer to Section 4.4, page 24), and confirm with OK.
43Standard programs4. Select Power Off Timer and press OK.5. Select the required time span after which the instrument will automatically be powered off if it is not used, and confirm with OK. 6. Press OK to confirm the entry. The instrument then returns to "Instrument Setup".5.3 Save, recall, send and delete data 5.3.1 The data logThe data log can store up to 2000 readings saved by the following programs: •Stored Programs, •Barcode Programs, •User programs, •Favorites, •Single Wavelength and •Multi Wavelength. A complete record of the analysis is stored, including the Date, Time, Results, Sample ID and Operator ID.5.3.1.1 Auto/manual data storageThe data storage parameter indicates whether data is to be stored automatically or manually (in which case the user has to decide which data to store).1. Press Data Log Setup in the "Instrument Setup" menu.•When the setting Auto Store: On is activated, the instrument will store all measurement data automatically. •When the setting Auto Store: Off is activated, the instrument will not store any measurement data. However, this setting can be changed on the results screen, via Options>Auto Store: On. The reading currently shown in the display is then stored.2. Specify the output format of data to be exported using Send Data Format. Select xml or csv.3. Press OK to confirm the entry. The instrument will return to "Instrument Setup".Note: When the instrument memory (data log) is full, the oldest data is automatically deleted allowing the new data to be stored.
44Standard programs5.3.1.2 Recall stored data from the data log1. Press Recall Data in the Main Menu.2. Press Data Log.A listing of the stored data is displayed.3. Press Filter: On/Off. The function Filter Settings is used to search for specific items.4. Activate On. The data can now be filtered using the following selection criteria.•Sample ID•Operator ID•Start Date•Parameteror any combination of the four.5. Press OK to confirm the selection. The chosen items are listed.
45Standard programs6. Press View Details to get more information.5.3.1.3 Send data from the data log Data is sent from the internal data log as an XML (Extensible Markup Language) file or as a CSV (Comma Separated Value) file, to a directory with the name DataLog on a USB mass storage device or a network drive. The file can then be processed using a spreadsheet program. The file name has the format: DLYear-Month-Day_Hour_Minute_Second.csv or DLYear-Month-Day_Hour_Minute_Second.xml.To send data to a printer, refer to Section 5.2.7.2, page 38.1. Plug the USB storage device into the USB type A port on the DR 3900, or connect the DR 3900 to a network drive (refer to Section 5.2.7.4, page 39).2. Press Recall Data from the Main Menu. 3. Select the data category to be transferred, e.g. Data Log. A list of the selected measurement data is displayed.4. Press Options and then the PC & Printer icon.
46Standard programs5. Select the data to be sent.The following options are available:• Single point: only this selected reading will be sent•Filtered data: only readings that correspond to the set filters will be sent• All data: all data in the selected data category will be sent.6. Press OK to confirm.Note: The number in parentheses is the total number of data sets assigned to this selection.5.3.1.4 Delete stored data from the data log1. Press Recall Data from the Main Menu.2. Press Data Log>Options>Delete.3. Highlight Single point, Filtered data or All data and press OK to confirm.Note: The number in parentheses is the total number of data sets assigned to this selection.5.3.2 Control charts for data from the AQA Log1. Press Recall Data in the Main Menu.2. Press AQA Log.A listing of the stored data is displayed.
47Standard programs3. Press Filter: On/Off. 4. Activate On. 5. Select a required parameter.6. Press OK to confirm the selection. The chosen items are listed.7. Press Options>Control Chart.8. Press Standard Control Chart to choose the control chart of the selected standard. The measured standard concentrations are displayed graphically with the control limits and date.
48Standard programs9. Press Range Control Chart to select the control chart of the range in % for multiple determinations. The range in % is displayed graphically with the control limits and date.5.3.3 Store, recall, send and delete data from wavelength scan and time courseThe instrument can store 20 datasets for the Wavelength Scan and 20 datasets for the Time Course. The data must be stored manually after it is displayed. 5.3.3.1 Data storage from wavelength scan or time course1. Press the Folder icon in the "Options" menu after a reading is taken.The Store Data list will be displayed.2. Press Store to save the current scan to the highlighted numbered line. Note: Scans can be overwritten.
49Standard programs5.3.3.2 Recall stored data from wavelength scan or time course1. Press Recall Data from the Main Menu.a. Select Wavelength Scan or Time Course to recall data.b. If a program is already running, press Options>More>Recall Data.2. Select the required data.3. Press Graph to look at the details.Note: Press View Summary to return to the "Recall Data" list.4. Press Table to look at the details.Note: Press View Summary to return to the "Recall Data" list.5.3.3.3 Send data from wavelength scan or time courseThere are two ways to recall sent data to a USB memory stick, printer or PC with Hach Data Trans.Option 1: 1. Press Recall Data in the Main Menu and then Wavelength Scan or Time Course. 2. Press Options and then the PC & Printer icon to send the data to a USB memory stick, to a printer or to a PC with Hach Data Trans.•When a printer is connected, select how to send the data to the printer (graph, table or both graph and table).
50Standard programs•If a USB memory stick is connected, the files will be automatically sent to the USB memory stick as xml or csv files in the file folder "WLData (data of a wavelength scan) or "TCData" (data of a time course).The file name will be formatted as: "ScanData_X.csv" (Wavelength Scan Data) or "TCData_X.csv" (Time Course Data).X = number of scans (1–20).For further processing use a spreadsheet program.Note: The message, "Data already present. Overwrite?" is displayed if the files are already stored. Press OK to overwrite the stored data.Option 2: 1. Press Wavelength Scan or Time Course and then Options>More>Send Data to send the data to a USB memory stick or to a printer.•When a printer is connected, select how to send the data to the printer (graph, table or both graph and table).•If a USB memory stick is connected, the files will be automatically sent to the USB memory stick as xml or csv files in the file folder "WLData (data of a wavelength scan) or "TCData" (data of a time course).The file name will be formatted as: "ScanData_X.csv" (Wavelength Scan Data) or "TCData_X.csv" (Time Course Data).X = number of scans (1–20).For further processing use a spreadsheet program.5.3.3.4 Delete stored data from wavelength scan or time course1. Press Recall Data in the Main Menu and then Wavelength Scan or Time Course.A listing of the stored data is displayed.2. Highlight any data to delete.3. Press Delete in the "Options" menu and press OK to confirm.
51Standard programs5.3.4 Data analysisConsistent use of the sample ID function is necessary for all data analysis functions. Only data with identical sample IDs can be used in data analysis functions. Use the Trends function to display a time-variation curve for the stored readings for each parameter and location. The concentration of the the corresponding parameter is shown graphically over time. Use the Ratio function to monitor the parameter relationships at a particular location and display them in a graph. 5.3.4.1 Trends1. Press Recall Data>Data analysis.2. Choose Trends and press OK to confirm.Alternatively, choose Trends from the toolbar.3. Press Options>New.
52Standard programs4. Select the required parameter and press Next to confirm.5. Select the required sample ID and press Next to confirm.6. Select the required operator ID and press Next to confirm.7. Select the required data analysis interval or the start and end date, and press Next to confirm.8. Set an upper and lower control limit and press Next to confirm.9. All of the selected functions are dispalyed in Trend Settings. Press OK to confirm.10. Select the required trend with the created settings from the list. Press Select.
53Standard programsThe trend can be displayed in several formats, as a line or a point.11. Select Information to see an overview of the parameter information.12. Select Options>Add Data to add more readings to the trend.13. Select Options>Ratio:Off to activate the Ratio function. The ratio corresponds to the first row of readings selected.5.3.4.2 RatiosThe Ratio function is based on the precise classification of samples by their sample locations using the optional accessories "LOC 100 RFID Locator" and "RFID Sample ID Kit". The precise sample location, date and time are attached automatically. Ratio analysis calculates a ratio between two or three parameters. The last defined parameter is represented by 1. The first or second parameters are calculated as a ratio against this, e.g. 4:2:1.1. Select Recall Data>Data analysis.2. Select Ratios.If a ratio analysis has already been carried out, a list of the defined analyses will be displayed.3. Press Options.
54Standard programs4. Press New, to define a new ratio analysis.5. Select the required sample ID and press Next to confirm.6. Select parameter I with the greatest concentration and press Next to confirm.7. Select parameter II with the middle concentration and press Next to confirm.
55Standard programs8. Select parameter III with the smallest concentration and press Next to confirm.9. Select the required data analysis interval. It is possible to select a fixed interval and an specific customised interval with start and end dates.10. Press OK to confirm.11. Choose an upper and lower control limit for the first and second parameters and press Next to confirm.12. All of the selected functions are displayed in Ratio Settings. Press OK to confirm.
56Standard programs13. Select the ratio with the defined settings from the ratio directory. Press Select.A table with the defined values is shown. Values outside the defined limits are displayed in red.
57Standard programs5.4 Stored ProgramsOver 200 pre-programmed processes can be recalled using the Stored Programs menu. Stored Programs do not include barcode tests.5.4.1 Select a saved test/method; entering user-specific basic data1. Press Stored Programs in the Main Menu to view an alphabetical list of stored programs with program numbers. The "Stored Programs" list will appear.2. Highlight the required test.Note: Select the program by name or scroll through the list using the arrow keys. Highlight the program or press Select by No. to search for a specific program number. Press OK to confirm. 3. Press Start to run the program. The respective measurement window is displayed.Note: All corresponding data (wavelength, factors and constants) is already preset.4. Follow the instructions for chemical procedures in the relevant procedures manual. Procedure manual is on CD with purchase of instrument.Note: To display the procedural instructions in the display, press the info icon. Not all programs have this option.5.4.2 Stored programs options1. Press Stored Programs in the Main Menu. Select the required process and press Start. 2. Press Options enter specific data. The following describes the options Table 4.
58Standard programsTable 4 Stored programs options Options DescriptionMore For further OptionsStore Off/On With the Store On setting, all measurement data is stored automatically. With the Store Off setting, no measurement data is stored.% Trans/Conc/Abs To switch to % transmittance, concentration or absorbance readingsSend Data icon / Send Data To send data to a printer, network drive, computer or USB memory stick (USB type A or Ethernet port)Timer iconThis functions as a stopwatch. It helps to make sure that the steps of an analysis are correctly timed (e. g. reaction times, wait times, etc., can be exactly specified). When the specified time has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the reading program.Dilution Factor Off/OnA corrective dilution factor can be entered in order to take account of certain properties.The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment. For example , if the sample has been diluted by a factor of 2, enter 2. The default setting of the dilution factor is turned off.Note: When a dilution is in effect, the dilution icon will appear on the display.Standard Addition This option enables the accuracy of the measurements to be checked. The procedural instructions for test parameters contain a detailed explanation of how to use this function.Standard Adjust The procedural instructions for test parameters indicate whether a standard adjustment is necessary and, if so, how to proceed.Chemical Form Some of the stored tests/methods allow to select the chemical form and the measuring range.Reagent BlankSome of the stored tests/methods include the "Reagent Blank" function. This enables the reagent blank value to be added to or subtracted from, the subsequent readings. The reagent blank value shifts the calibration curve along the y-axis, without changing the shape or gradient of the curve. The effect corresponds to a y-axis intercept of the calibration straight line. This is clarified by the following formula: concentration =[conc. factor) x Abs] - (reagent blank)Save as User Program To store the selected parameters as a User Program, refer to Section 6.1, page 73.Multiple determination This option allows the multiple determination of a sample.Recall Data To recall saved measurement data or time courses, refer to Section 5.3, page 43.Instrument Setup mode Basic data for the instrument, refer to Section 5.2, page 27.
59Standard programs5.4.3 Use of program timersSome procedures do not require the use of timers. Other procedures require several timers. These timers are pre-programmed into each Stored Program, along with a description of the activity to be performed during the timed period.1. Press the Timer icon.2. Press OK to start the first timer.The timer will count down on the screen. 3. To start the next timed activity for the Stored Program, press the Timer icon and OK.Note: Press Close to view the reading screen while the timer is running. The time will be shown left side bottom instead of the date.Note: Press Cancel to stop the timer midway through the countdown.Note: The timer will beep when the timed interval ends.A general purpose timer is also available in many programs. Type the timer icon and select General Timer. Enter the length of the timed interval and press OK to start the timer. The timer will beep when the timed interval ends.5.4.4 Set the dilution factorThe Dilution Factor function is used to calculate the original concentration of a sample that has been diluted by a known ratio. 1. Press Options>More…>Dilution Factor.The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment. For example, if the sample has been diluted by a factor of 2, enter 2. The default setting of the dilution factor is turned off.2. Press OK to confirm. Press again OK.
60Standard programsNote: When a dilution is in effect, the dilution icon will appear on the display.Note: If using undiluted samples, set the dilution factor off again.5.4.5 Run a standard adjustThe Standard Adjust functions allows the calibration curve for a stored program to be adjusted based on analysis of a known standard solution. The Accuracy Check section of written procedures often suggests a standard solution concentration for this purpose. 1. Follow the entire procedure, using a known standard for the sample.2. After reading the concentration, press Options>More>Standard Adjust.3. Press On.The "Current Reading" will show the concentration. The box on the right will show the default standard value for the test, as mentioned in the procedure. 4. To set the standard concentration, press the button and enter the new value. Press OK to confirm.5. Press Adjust to enable the Standard Adjust. The Standard Adjust icon will appear.Note: The adjustment must be within certain limits, which vary with each program. The allowable percentage is shown after "Adjustment“.5.4.6 Set the chemical formSome Stored Programs allow a variety of chemical forms tobe selected.Press the unit (e. g. mg/L) or the chemical representation of the evaluation form (e. g. Al3+). A list of available evaluation forms appear. Select the required form by pressing the corresponding entry in the list.Note: To exit from the program, the evaluation form reverts to the standard setting.
61Standard programs5.4.6.1 Change of the default setting of the chemical form1. In the result display, press Options>More>Chemical Form.2. A list of available evaluation forms appear. Select the new default setting.3. Press Save as Default. The current result and all further readings will be displayed in the new chemical form.5.4.7 Run a reagent blankSome of the stored tests/methods include the "Reagent Blank" function. This enables the reagent blank value to be measured and then taken into account in calculating the reading result.Reading/analysis of a reagent blank: 1. Prepare the test corresponding to the specification/procedural instructions. Use distilled water instead of a sample to determine the reagent blank value.2. Select the test. Insert the zero solution into the cell compartment, if required by the procedural instructions. Press Zero.3. Insert the prepared sample cell into the cell compartment. Press Read. The result will be displayed.4. Press Options>More>Reagent Blank.5. Press On to highlight the Reagent Blank function.6. The concentration shown on the button is the reading of the reagent blank. To use this value for more analyses of this parameter, press OK. 7. To change the value displayed on the button, press the button. Use the alphanumeric keypad to enter the new value.8. Next press OK.Note: The "Reagent Blank" function is deactivated when the reading program is left. To use the same blank value later for other tests using the same reagent lot, enter the value per step 7. Note: The results calculated using the reagent blank value must lie within the limits of the measuring range of the test/method.
62Standard programsNote: The reagent blank icon is shown in the result display (refer to arrow) when the function is active.5.4.8 To carry out a multiple determinationA sample can be measured several times using the multiple determination functions. 1. Before reading the concentration, press Options>More>Multiple Determination.2. Press On.The number of multiple determinations is shown under "Measurements". 3. To change the value displayed on the button, press the button. Use the alphanumeric keypad to enter the new value. Press OK to confirm.4. The Multiple Determination icon will be shown on the display.5.4.9 Analysis of samples1. Press Stored Programs and select a program.Note: The procedural instructions are shown in the display by using the info icon when available.2. Insert the zero solution cell into the cell compartment.3. Press Zero.
63Standard programs4. Remove the zero solution cell from the cell compartment. Insert the sample cell into the cell compartment.5. Press Read. The result will be displayed.Note: To define a sample dilution, press the Dilution key in the toolbar.6. For data storage, refer to Section 5.3.1, page 43.5.4.10 Update/edit tests (not available on all models)5.4.10.1 General advices for program update and editFor a manual test update and for a new programming of a test, refer to Section 5.4.10.2, page 63 and Section 5.4.10.3, page 64.Note: Only in very few cases does the revision of a test require the test specifications to be updated.To update and edit barcode tests, refer to Section 5.5.5, page 68.5.4.10.2 Manual update of test dataWhen a test is selected the instrument automatically sets the reading wavelength and factors.Tests are continuously reviewed for simplified handling and to make sure current legal requirements are followed. This can lead to a change in the procedural instructions or an alteration to the factors. If such an alteration is indicated on the test packaging, update the instrument data according to the data supplied on the process specification inside the test packaging.For updating from the Internet, refer to Section 5.5.5.4, page 71.1. Press Stored Programs in the Main Menu and select a program. 2. Press Program Options and then Edit.
64Standard programsThe Data list of the corresponding test will appear.The process specification of the corresponding test contains the new data (Wavelength, Factors, reading Ranges, Conversion Factor for chemical Form etc.).3. Highlight the corresponding line in the data overview and press Edit.4. Press OK and then Store after editing the test.5.4.10.3 Program a new test 1. Press Stored Programs in the Main Menu.2. Press Program Options and then New.3. Use the alphanumeric keypad to enter the Program Number indicated in the working procedure.An overview of the data for the test to be programmed is displayed.The process specification of the corresponding test contains the new data (Wavelength, Factors, reading Ranges, Conversion Factor for chemical Form etc.).4. Highlight the line containing the test specification that is to be programmed, then press Edit and enter the data in the appropriate program lines.5. Press OK and then Store after editing the test.5.4.11 Add stored programs to the favorite programs listThe "Favorites" menu simplifies test selection by creating a list of the most frequently used tests from the Stored Programs andUser Programs.1. Press Stored Programs in the Main Menu. The "Stored Programs" list will appear.2. Highlight the selection by pressing it or Select by Number to search for the program by number.3. Press Add to Favorites and press OK to confirm.The program can now be selected from "Favorite Programs" menu in the Main Menu.
65Standard programs5.5 Barcode ProgramsA special barcode reader in cell compartment (1) automatically reads the barcode on the 13 mm cuvette/vial as the cuvette/vial completes a single rotation. The instrument uses the barcode identification to automatically set the correct wavelength for the analysis and calculates the result immediately with the help of the stored factors. In addition, readings are recorded at 10 different positions during a rotation. A special outlier-elimination program is run and then the average of the measured values is calculated. Cell and round cell faults and contaminations are detected and therefore a very precise result is determined.5.5.1 Complete a barcode test1. Insert the light shield into cell compartment (2).2. Prepare the barcode test according to the process specification, and insert the cell in cell compartment (1).•When a coded cell is placed in cell compartment (1) (Figure 3, page 19), the corresponding reading program is automatically activated in the Main Menu.•Otherwise, press Barcode Programs in the Main Menu and insert a zero cell (depending on process specification) into cell compartment (1). Note: For further information about the Help Guide ("Information" icon), refer to Appendix A, page 143.The reading is started automatically and the results are displayed.Note: To define a sample dilution, press the Dilution key in the toolbar.To evaluate other cell tests and other parameters, insert the prepared cell into the cell compartment and read off the result.Note: The control bar displayed on the right of the screen shows the relationship of the reading result to the measuring range. The black bar shows the measured result independently of any dilution factor that was entered.
66Standard programs5.5.2 Select the chemical evaluation formThe chemical form of the test result of a number of parameters can be selected individually.1. In the result display, press the unit (e. g. mg/L) or the chemical representation of the evaluation form (e. g. PO43––P).A list of available evaluation forms appears.2. Select the required form from the list. Press OK to confirm.Another way of changing the standard setting is:1. In the result display, press Options>More>Chemical Form.A list of available evaluation forms appear.2. Select the required chemical form and press OK to confirm.Note: The selected chemical form is displayed, but does not become the default. To change the default, refer to Section 5.5.2.1.5.5.2.1 Change of the default setting of the chemical form1. Insert the zero cell or sample cell (corresponding to the process specification) into the cell compartment.2. In the result display, press Options>More>Chemical Form.3. A list of available evaluation forms appear. Select the new default setting. 4. Press Save as Default. The current result and all further readings will be displayed in the new chemical form.5.5.3 Basic test-specific and sample-specific data settingsPress Options to change test or sample specific settings.
67Standard programsTable 5 Barcode program options Options DescriptionMore For further OptionsStore Off/On Store: On stores all measurement data automaticallyStore: Off does not store any measurement data.Abs % Trans To switch to % transmittance or absorbance readingsSend Data icon / Send Data To send data to a printer, computer or USB memory tick (USB A) or network.Timer iconThis functions as a stopwatch. It helps to make sure that the steps of an analysis are correctly timed (e. g. reaction times, wait times, etc., can be exactly specified). When the specified time has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the reading program.Dilution Factor Off/OnA corrective dilution factor can be entered in order to take account of certain properties.The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment. For example , if the sample has been diluted by a factor of 2, enter 2. The default setting of the dilution factor is turned off.Note: When a dilution is in effect, the dilution icon will appear on the display.Note: If undiluted samples are used, set the dilution factor off.Chemical Form Some of the stored tests/methods allow to select the chemical form and the measuring range.Reagent BlankThe function "Reagent Blank" is available for several barcode tests. This enables the reagent blank value to be added to or subtracted from, the subsequent readings. The reagent blank value shifts the calibration curve along the y-axis, without changing the shape or gradient of the curve. The effect corresponds to a y-axis intercept of the calibration straight line. This is clarified by the following formula: concentration =[conc. factor) * Abs] - (reagent blank)Edit To modify an existing programLot information All relevant manufacturing data is stored here.Save as User Program To store the selected parameters as a User Program, refer to Section 6.1, page 73.Multiple determination This option allows the multiple determination of a sample.Recall measurement data Recall saved measurement data, wave-length scans or time courses, see Section 5.3, page 43.Instrument Setup mode For basic instrument data, refer to Section 5.2, page 27.
68Standard programs5.5.4 Sample blankTurbidity and colour in the sample matrix can falsify the results of a photometric analysis. The interference factors come from the sample or are created by reactions with the reagents.The influence of turbidity and/or colour can be eliminated or reduced by taking a sample blank reading.After the sample reading has been carried out, a special cell (LCW919) with the sample blank is inserted in the cell compartment. Measurement is automatic. The sample reading is then corrected by adding or subtracting the blank value. The result is shown in the display with the Blank Correction icon.Some barcode tests do not require a sample blank value to be determined, as turbidity and colour are dealt with during the test procedure.Note: For further information about the Help Guide ("Information" icon), refer to Appendix A, page 143.5.5.5 Update/edit barcode tests5.5.5.1 Manual update of a barcode testNote: Only in very few cases does the revision of a test require all test specifications to be updated.The various data types that determine a barcode test are described in Table 6.Table 6 Program update options Program point DescriptionName Name of the analysis parameter Version An abbreviation assigned by the user or the version number, etc. is entered hereMeasurement process Exact definition of the test: the number of wavelengths at which readings are carried out, the number of absorbance readings needed, the keys to be used, any waiting periods between readings, etc.Formula Definition of the evaluation formulas with which the test result is calculatedVariables The number of variables shown in the display depends on the definition of the measurement process and the formulas. Input of the numerical values of the wavelengths, factors, constants, etc.Timer 1, Timer 2, Timer 3, Timer 4This function can be used to enter abbreviations and defined times for up to four timers. Highlight the corresponding line and press Edit.The timers are activated or deactivated with the control boxes on the left of the display. In the next column, a selection can be made from a list of names that describe the corresponding work step. In the third column, the times for each active timer are entered.
69Standard programsEnter the NameCheck the process specification in advance to determine whether this point needs to be changed.1. Highlight the line containing Name and press Edit. Use the alphanumeric keypad to enter the name indicated in the process specification.2. Press OK to confirm.Enter the Version1. Highlight the line containing Version and press Edit. Use the alphanumeric keypad to enter the name indicated in the process specification.2. Press OK to confirm.Enter the measurement processFor detailed information on entering the measurement process, refer to Section 6.1.2, page 82. Check the process specification in advance to determine whether this point needs to be changed.1. Highlight the Measurement process line in the data overview and press Edit.2. Press Edit, select the sequence to be editted and press Delete.3. Press New and use the alphanumeric keypad to enter the process indicated in the process specification.Enter the Formula, Concentration Units, Designation, Measuring RangesFor detailed information on entering the Formula, refer to Section 6.1.2, page 82.Check the process specification in advance to determine whether this information needs to be changed.1. Highlight the Formula line in the data overview and press Edit.2. Select the formula; press Edit, and enter the data (refer to process specification) (for C1=, C2=, units, name, measurement range limits, etc.). Press OK to confirm the entry.
70Standard programsEnter the Variables (Factors, Wavelength and Coversion Factors)For detailed information on entering the Variables, refer to Section 6.1.2, page 82.Check the process specification in advance to determine whether this point needs to be changed.1. Highlight the Variables line in the data overview and press Edit.1. Select the variable; press Edit, and enter the data (refer to process specification) using the alphanumeric keypad (for F1, F2, 1, U1 etc.). Confirm each entry with OK.Abbreviation of Variables:F1: Factor 1F2: Factor 21: Wavelength 1U1: Conversion factor 1 for the first chemical formU2: Conversion factor 2 for additional chemical forms, etc..Timer 1, Timer 2, Timer 3 and Timer 4: The timers are activated or deactivated with the control boxes on the left of the display. In the next column, a selection can be made from a list of names that describe the corresponding work step. In the third column, the times for each active timer are entered.2. When finished programming, press Store and then Cancel to return to the "User Programs" menu.3. Press Start to begin the test with the new data.5.5.5.2 Manual check/revision of test dataIf an incorrect entry was made during the data update and is not recognized via the barcode of the cuvette/sample cell (e. g. an incorrect measuring range), another option is available for checking and if necessary revising, the test data.1. Insert the light shield in cell compartment (2).The instrument needs to be at the "Main Menu". Alternatively, select Barcode Programs in the Main Menu.2. Insert the zero cell or sample cell (corresponding to the process specification) into cell compartment (1).3. Press Options>More>Edit.A data overview is displayed, with the specifications of the test that is to be revised.
71Standard programs4. Compare the displayed data with the data in the process specification and edit the displayed data in accordance with the process specification.5.5.5.3 Update via the InternetUpdate via USB memory stickTo obtain the software for the update from the Internet at www.hach.com:1. Go to http://www.hach.com.2. Select download resources>software downloads.3. Enter DR 3900 in "Search for documents".4. Locate the appropriate download file.5. Open the ZIP file and save the files to the USB memory stick or PC. The files have to be unzipped prior to saving on USB or PC.6. Press Instrument Update in the "System Check" menu.7. Connect the USB memory stick to the USB port (type A) on the instrument, refer to Section 3.5, page 18.8. Press OK. The link is established automatically and the software is updated.9. Press OK to return to the "System Check" menu.Update via the web function1. Connect the instrument to the network via an Ethernet cable.2. Activate the Internet connection by selecting Instrument Setup>PC & Printer (Section 5.2.7.4, page 39).3. Press Web Function on the toolbar. 4. Press Instrument Update.The update will start automatically.Note: When the instrument software has been updated, a prompt to restart the instrument is displayed.5.5.5.4 Program a new test1. The instrument needs to be at the "Main Menu" or press Barcode Programs in the Main Menu.2. Insert the zero cell or sample cell (corresponding to the process specification) into the cell compartment.
72Standard programsThe message "Program not available" is shown, followed by a data overview with the specifications of the test that is to be revised.The process specification of the corresponding test contains the new data (Wavelength, Factors, Reading Ranges, Conversion Factor for chemical form etc.).3. Highlight the corresponding line including the specifications of the test that is to be programmed and press Edit.4. To finish programming, press Store and then Cancel to return to the Main Menu.5. Press Start to begin the test with the new data.
73Section 6 Advanced operations6.1 User programsUser programs provide the opportunity to complete "made to measure" analysis.The "User Programs" database is empty when the instrument leaves the factory and is used to accommodate programs created by users for specific needs. User programs with 13 mm round cells can only be performed with the empty cells LCW906. Here are a few examples of entries:•Programming of user-created procedures. The analysis procedure must be developed first, before it can be programmed. The user must define or determine the program sequences, absorbance formulas, reading wavelengths, factors, measuring range limits, etc.•Modified tests•Assignment of user programs to the "Favorites" menu for frequently used tests.•Creation of a specific selection of methods and tests.1. Select User Programs in the "Main Menu"2. Select Program Options. The Program Options menu contains several input and editing options (Table 7):Table 7 Program options for user programsOptions DescriptionNew Select New to program a new user program.Note: The first time Program Options is selected, only the New option is available. The other options remain inactive (grey) until the first program has been created.Add to favorites Select Add to favorites to add an existing user program to the Favorites list.Edit Select Edit to modify an existing programDelete Select Delete to remove a program from the list of user programs. The program will be simultaneously deleted from the Favorites list.
74Advanced operations6.1.1 Program a user methodAll input steps and their significance and options are explained in the following sections.1. Select New in the "Program Options" menu.Program Number: Specific test number, with which the program can subsequently be called up from the selection list in the User Programs menu or the Favorites menu.2. Use the alphanumeric keypad to enter a program number between 9000 and 9099. The lowest available number appears automatically.3. Press OK.Note: If a program number is already allocated to another user program, a query appears asking whether the present program is to be replaced. If OK is pressed, the existing program is overwritten.Program name: 4. Use the alphanumeric keypad to enter a program name. The name can be a maximum of 28 characters long.5. Press Back to go back to the previous program point or press Next to continue with the input of the program data.Program type:6. Select the required program type (Table 8) and press Next.7. If the options Single Wavelength (Section 6.1.1.1, page 75) or Multi-Wavelength (Section 6.1.1.2, page 76) are selected, define the programming parameters:•Unit •Wavelength •Calculation formula•Wavelength 1x •Concentration factor Kx •Concentration solution •Chemical form •Calibration equation For more information on free programming parameters refer to Section 6.1.2, page 82.
75Advanced operations6.1.1.1 Single wavelength settingsThe following parameters can be defined when selecting the single wavelength mode: Units: 1. Select the required unit from the list.2. Press Continue.Note: Units of measure not included in this list can be added in the edit program under Program Options, Edit. Select Units, Edit and then New.Wavelength (single wavelength program type): 1. Enter measured wavelength. The entered wavelength must be in the range from 320–1100 nm.2. Press Continue.Concentration resolution (number of decimal places)1. Select the required number of decimal places to be displayed after the decimal point from the displayed list.2. Press Continue.Table 8 Program descriptionsProgram type DescriptionSingle Wavelength Readings at a defined wavelengthMulti Wavelength In the Multi Wavelength mode, absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums, differences and relationships.Free programming A high degree of flexibility results in individual options to create a user program. The free programming is an expanded form of test or method programming.
76Advanced operationsChemical form:1. Enter the chemical formula used in the display to represent the analysis parameter.2. Press Continue.6.1.1.2 Multi wavelength settingsWhen selecting the Multi-Wavelength mode, the following parameters can be defined: Units: 1. Select the required unit from the list.2. Press Continue.Note: User-specific unit not included in this list can be added in the edit program under Program Options, Edit. Select Units, Edit and then New.Absorbance formula (Multi Wavelength program type): Define the wavelengths here and the coefficients used in the formula. The absorbance formula defines the calculation for the multi wavelength reading. 1. Press Formula.2. Select the formula relevant to the programming from the displayed list.3. Press OK to confirm the selection.List of available absorbance formulasA1 is the absorbance at wavelength 1, A2 is the absorbance at wavelength 2 and so on K1 is the factor at wavelength 1, K2 is the factor at wavelength 2 and so on If a subtraction has to be performed, the factors can be entered with a minus sign.
77Advanced operationsWavelength x:1. Press the x buttons. The number of wavelengths results from the calculation formula.2. Enter the required wavelengths.3. If necessary, repeat until all values for the formula have been entered. The wavelengths must be in the range from 320–1100 nm. 4. Press OK to confirm.Concentration factor KxThe concentration factor Kx is a multiplication factor to convert absorbance values into concentration values.1. Press the Kx buttons.The number of the concentration factors results from the calculation formula.2. Enter the required factor.3. If necessary, repeat until all the values for the formula have been entered. 4. Press OK to confirm.5. Press Continue.Note: Up to 5 digit place values can be entered. A maximum of 4 decimal places are possible.Concentration resolution (number of decimal places)1. Select the required number of decimal places to be displayed after the decimal point from the list.2. Press Continue.Chemical form:1. Enter the chemical formula used in the display to represent the analysis parameter.2. Press Continue.
78Advanced operations6.1.1.3 Calibration settings for single and Multi Wavelength modeA method is calibrated by determining the absorbance values of several standard solutions of known concentration.Create and save a calibration curve by entering values or measuring standards or entering formulas (Table 9).Calibration by "Enter values"1. Select Enter values.2. Press Continue.3. Press +.4. Enter the standard concentration.5. Press OK to confirm.6. Enter the corresponding absorbance value.7. Press OK to confirm.8. If necessary, repeat until all values have been entered.Note: To change a value in the table, mark the corresponding row. Press the key of the unit (e. g. mg/L) or Ext. Enter the changed value.Table 9 Calibration settingsOption DescriptionEnter valuesCreate a calibration table by entering the concentration values and the corresponding absorbance values of the standard solutions be measured. The absorbance values are applied in a diagram against the concentrations of the standard solutions. The calibration curve is graphically presented (page 78).Measure standardsCreate a calibration table by entering the concentration values of the standard solutions to be measured and then determining the corresponding absorbance values by measuring the standard solutions. The absorbance values are applied in a diagram against the concentrations of the standard solutions. The calibration curve is graphically presented (page 79).Enter formulaIf it is possible to determine the calibration curve from the mathematical relationship between concentration and absorbance through linear regression or another curve adaptation, the respective formula (linear, polynomial 2nd or 3rd order can be selected from a list and the corresponding factors can be entered (page 80).
79Advanced operations9. Press Graph.The entered data is shown as a calibration curve.Note: The correlation coefficient (r2) is shown on the left below the axes.The linear equation corresponds to the standard setting. 10. Press Next graph.The presentation changes to the polynomial curve of the second order.11. Press Next graph.The presentation changes to the polynomial curve of the third order.12. Press Force 0 to change the setting from Off to On. The curve then passes through the origin of the coordinate system.Note: This may have an adverse effect on the correlation coefficient (r2).13. Press on table to return to the values table.14. When the table has been completed and the curve type has been chosen, press Done when the graph is displayed or Exit when the table is displayed. Go to Section 6.1.1.4, page 81.Calibration using "Measure standards"1. Press Measure standards. 2. Press Continue.3. Press +.4. Enter the standard concentration.5. Press OK to confirm.6. If necessary, repeat until all values have been entered. A maximum of 24 values can be entered.Note: To change a value in the table, mark the corresponding row. Press the key of the unit (e. g. mg/L). Enter the changed value.7. Insert the zero solution into the cell compartment. 8. Press Zero.9. Mark the row with the first standard concentration and insert the respective cell.10. Press Read. 11. If necessary, repeat until all standard solutions are measured.
80Advanced operationsThe entered and measured data is displayed in the table.Note: To delete a standard concentration, highlight the appropriate line and press the Delete icon.The timer icon shown in the display helps to make sure, when necessary, that the steps of an analysis are correctly timed (e. g. reaction times, wait time, etc., can be exactly specified). When the specified time has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the reading program.12. Press Graph.The entered and measured data is shown as a calibration curve.Note: The correlation coefficient (r2) is shown on the left below the axes.The linear equation corresponds to the standard setting. 13. Press Next graph.The presentation changes to polynomial curve of the second order.14. Press Next graph.The presentation changes to polynomial curve of the third order.15. Press Force 0 to change the setting from Off to On. The curve then passes through the origin of the coordinate system.Note: This may have an adverse effect on the correlation coefficient (r2).16. Press Table to return to the values table.17. When the table has been completed and the curve type has been chosen, press Done when the graph is displayed or Exit when the table is displayed. Go to Section 6.1.1.4, page 81.Calibration using "Enter formula"1. Press Enter formula.2. Press Continue.
81Advanced operations3. Press the Formula key.A list of available formulas (linear and 2nd and 3rd order polynomial order) is displayed. Up to 4 coefficients can be entered, depending on the selected formula. 4. Press the required formula.According to the selected formula, the required factors (a, b, c...) are shown. 5. Press the factors keys and enter the corresponding values. 6. Press OK to confirm each factor entry.Note: The coefficients can have 5 digits and can have a positive or a negative sign.7. Press OK to confirm.6.1.1.4 Store a user programThe input of the basic data is complete. An overview of the variable program data is displayed.1. To enter more specifications or change existing ones, highlight the appropriate line and press Edit.2. Select Store to save the user program.6.1.1.5 Additional user-defined parameters and functionsIn addition to the previously defined basic data, additional parameters and functions can be defined for user programs:•measuring range upper and lower limits•timer functions•chemical formsUpper and lower limits of the measuring rangeIt is possible to enter a maximum (upper) and minimum (lower) reading value. An error message is displayed if a reading is above the upper limit or below the lower limit.1. Mark the Upper limit or Lower limit row. 2. Press Edit.3. Select On.4. Press 0.000 and enter the measuring range limit. 5. Press OK to confirm.
82Advanced operationsTimer 1 / Timer 2 / Timer 3 / Timer 4:Up to four time intervals can be defined as timers with this function. Descriptions of the individual work steps, such as shaking, waiting, and stirring can be allocated to the timers.1. Mark the Timer x row.2. Press Edit.Activate or deactivate the timers using the check box in the lower area of the display. 3. Activate a timer.4. Press Timer. 5. Select the designation from the displayed list that describes the respective process and confirm with OK.6. Press 00:00.7. Enter the required time interval (in mm:ss) and confirm with OK.Chemical form 2 / chemical form 3 / chemical form 4:If a chemical form 1 was defined, up to three additional alternative forms can be entered.1. Mark the Chemical form x row. 2. Press Edit.Activate or deactivate the chemical form using the check box in the left area of the display.3. Press the left key to enter an additional chemical form. 4. Press OK to confirm. 5. Press the right key to enter the conversion factor for this additional chemical form. 6. Press OK to confirm.7. Press Store to save the program data. Press Cancel to return to the Main Menu.6.1.2 "Free programming" program typeFree programming is an advanced option for entering original user-developed methods. The data overview contains the specifications of the programmed tests. Each entry option can be activated by pressing the respective row and can be changed using Edit (refer to Table 10).1. Select New in the "Program Options" menu.
83Advanced operationsProgram Number: Specific test number, with which the program can subsequently be called up from the selection list in the User Programs menu or the Favorites menu.2. Enter a program number between 9000 and 9099. The lowest available number appears automatically.3. Press OK.Note: If a program number is already allocated to another user program, a query appears asking whether the present program is to be replaced. If OK is pressed, the existing program is overwritten.Program name: 4. Use the alphanumeric keypad to enter a program name. The name can be a maximum of 28 characters long.5. Press Back to go back to the previous program point or press Next to continue with the input of the program data.Program type:6. Select Free programming and then press Continue.7. Highlight the line containing the program point that is to be edited or defined and press Edit.
84Advanced operations6.1.2.1 Measurement processThe measurement process defines the handling and the readings of the test:•At which and how many wavelengths should readings be completed•How many absorbance readings must be completed?•When should the zero reading and the sample reading be completed?•Are waiting times necessary between readings?•Should individual program sequences be repeated?The elements of a measuring sequence, such as zero and sample readings and the timer(s) (reaction times, waiting times, etc.) are individually defined.6.1.2.2 Enter a new element of a measuring sequenceNote: Each component of the measurement process must be entered in the order in which it will be completed.1. Mark the Measurement process row in the data overview.2. Press Edit.3. Press Edit.4. Press New.Table 10 Definitions of the program points Program point DescriptionName Name of the analysis parameter Version An abbreviation or version number assigned by the user is entered here.Measurement process Exact definition of the test: the number of wavelengths at which readings are carried out, the number of absorbance readings needed, the keys to be used, any waiting periods between readings, etc.Formula Definition of the formulas with which the test result is calculated.Variables The number of variables shown in the display depends on the definition of the measurement process and the formulas. Input of the numerical values of the wavelengths, factors, constants, etc.Timer 1, Timer 2, Timer 3, Timer 4This function can be used to enter abbreviations and defined times for up to four timers. Highlight the corresponding line and press Edit. The timers are activated or deactivated with the control boxes on the left of the display. In the next column, a selection can be made from a list of names that describe the corresponding work step. In the third column, the times for each active timer are entered.
85Advanced operationsContent and definition of the keys[Z] key / Zeroing1. Press [Z] to program a zero reading. 2. Press OK to confirm. 3. Press New.4. Press Zeroing and enter the wavelength for which the zero reading is to be performed. 5. Press OK and confirm the entry.6. Press OK.7. If zero readings are to be carried out at a number of wavelengths, repeat the steps starting with 1 for each wavelength.Note: The entered reading sequence is displayed.Process Timer key1. Press New.2. Press Process timer to enter wait, reaction or handling times. 3. Press OK and confirm the entry.4. Press OK.Note: This time is integrated into the measurement process.Note: The entered reading sequence is displayed.[R] key / Reading...1. Press New.2. Press [R] to program a reading of the substance that is to be analyzed. 3. Press OK to confirm.4. Press New.5. Press Read...and enter the wavelength for which the measurement is to take place. 6. Press OK and confirm the entry.7. Press OK.8. If readings are to be completed at a number of wavelengths, repeat the steps starting with 1 for each wavelength.Note: The entered reading sequence is displayed.
86Advanced operationsKey { }Elements of the reading sequence that are to be repeated are placed in brackets.The left bracket { marks the start of the sequence to be repeated and the right bracket } marks the end.Note: The showing the right bracket remains inactive until a left bracket is entered.1. To start the repetition sequence, press New.2. Press {.3. Press the key that defines the sequence that is to be repeated: [Z] or [R].4. Press OK.5. Press New.6. Press Zeroing or Read..., and enter the wavelength for which the measurement is to occur.7. Press OK and confirm the entry.8. Press OK.Define additional steps that are to be included in the repetition sequence.9. At the end of the repetition sequence, press New.Define the last step that is to be included in the repetition sequence. Do not press OK.10. Press }.11. Press OK.Note: If an action such as a zero reading recurs at different stages of a reading sequence, the series of actions is numbered sequentially (e. g. Z1, Z2, etc.).Delete an element of a measuring sequence1. Mark the respective row.2. Press Delete. The element is deleted.Insertion of an element of a measuring sequence1. Mark the row below the reading sequence to be inserted.2. Press New.A new element can be entered at the selected position.3. When the input is complete, press OK in the "Measurement Process" display. The measurement process is displayed in the overview.4. Press OK.
87Advanced operations6.1.2.3 Enter the calibration formula (evaluation formula)The calibration formula (evaluation formula) defines the calculation and display of intermediate and final results. The previously defined elements of the measuring sequence are the basis for calculating the concentrations.Enter calibration formula C11. Mark the Formula row in the data overview.2. Press Edit.3. Mark the C1 row: Off.4. Press Edit. 5. Mark C1: Out.6. Press Edit. The display changes to C1: On.7. Mark the next row C1=, to define the formula.8. Press Edit. Refer to Table 11 for detailed information on the Edit formula keys.Note: The evaluation formula is built up successively in the display in accordance with the input.Note: The arrow key deletes the most recently entered element of the formula.
88Advanced operationsTable 11 Edit formula key descriptions Screen Key DescriptionAbs/VariablesPress the Abs/Variables key to select, from the displayed list, the required element of the defined measuring sequence and therefore the corresponding reading wavelength, so that this can be taken into account in the formula.New Number Press New Number to enter a new factor or constant.+ –  ×Press +–x to enter a mathematical operation. Select the operation and press OK to confirm.The available choice of mathematical operations depends on the defined formula.This means that functions such as "( )" or "ln"/"log" etc. are only active if a term in parentheses or the calculation of a logarithm is mathematically permissible in the defined formula (this also applies to the basic mathematical operations).The following mathematical operations are available:•+ (Addition)•- (Subtraction)• (Division)•x (Multiplication)•^ (Exponent)•Ln (Natural logarithm)•Log (Common logarithm)
89Advanced operationsEentering the next calibration formula (C2 or Cn)1. Mark C2: Out.2. Press Edit.3. Mark C2: Out.4. Press Edit. The display switches to C2: On.5. Mark the next row C2= to define the formula.6. Press Edit.In addition to the keys described in Table 11, only one other function is available:Select Conc. key 1. If an already defined formula, in this case C1, is to be taken into account in the formula for C2, press Select conc.2. Enter the numbering of the equation (e. g. 1 for C1).3. Press OK to confirm the entry.Cn can now be linked with a mathematical operation.Note: The Cn concentrations that are to be calculated are numbered in sequence: C1, C2, C3, etc.Note: When the first Cn formula has been defined, the Formula list is automatically extended by Cn+1.>=<Press >=< to include logic statements/links/conditions in the formula. The following functions are available:= (Equal to), < (Less than), > (Greater than), <= (Less than or equal to), >= (Greater than or equal to), IF, THEN, ELSEWhen the evaluation formula C1 has been entered completely, press OK to confirm. Press OK again to return to the Formula display.When the formula C1 has been entered and confirmed, the parameter name, upper and lower limits of the measuring range and display result (yes, no) can be entered.Table 11 Edit formula key descriptions(Continued)Screen Key Description
90Advanced operations6.1.2.4 Enter variables1. Mark the Variables row in the data overview.2. Press Edit.3. Select the variable to be edited.4. Press Edit. and enter the data (refer to process specification) (for F1, F2, 1, U1 etc.) 5. Press OK to confirm each entry.Abbreviation of Variables:F1: Factor 1F2: Factor 2 1: Wavelength 1U1: Conversion Factor 1 for the first chemical formU2: Conversion Factor 2 for an additional chemical form etc.6.1.2.5 Save a free programming user program1. Press Store to save the entered data. The data can be stored under any data point (measurement sequence, formula, timer, etc.).6.1.3 Select a user program1. Press User Programs in the Main Menu.An alphabetical list of user programs with program numbers is displayed.Note: Use the scroll bar to scroll through the list quickly.Note: Highlight the selection by pressing it or Select by Number to search for the program by number.2. Press Start to run the program.6.1.4 Add, edit and delete user programs from the favorites listThe most frequently used tests/methods in the "User Programs" menu can also be added to the list of favorites to simplify their selection.
91Advanced operations1. Press User Programs in the Main Menu. The User Programs list will appear.2. Highlight the selection by pressing it or Select by Number to search for the program by number.3. Press Program Options.Using Add to favorites, add the selected program to the favorite list.Use Edit to edit the selected program.Use Delete to delete the selected program.Note: If the stored program is deleted in User Programs, it will also be deleted in Favorites Programs. 6.1.4.1 Add to favourites1. Press Add to favourites. 2. Press OK to confirm.The program is added to the Favorites.6.1.4.2 Edit1. Press Edit.2. Press OK to confirm.An overview of the specifications of the programmed test is displayed. More information about the input options is provided in the Section 6.1.2, page 82.
92Advanced operations6.1.4.3 Clear Entry1. Press Delete.2. Press OK to confirm.The program will be deleted from the list of user programs.Note: If the stored program is deleted in User Programs, it will also be deleted in Favorites Programs.6.2 FavoritesThe most frequently used tests/methods in the Stored Programs menu and the User Programs menu can also be added to the list of favorites to simplify their selection.To add Stored Programs and/or User Programs to the favorites list or the favorite programs, refer to Section 6.1.4, page 90.6.2.1 Recall a favorite program1. Press Favorite Programs in the Main Menu. The "Favorite Programs" list will appear.2. Highlight the selection by pressing it.Note: Use the scroll bar to scroll through the list quickly.Note: Press Select by Number to search for the program by number. Use the alphanumeric keypad to enter the test number (program number) and confirm the input by pressing OK.3. Press Start.6.2.2 Delete a favorite program1. Press Favorite Programs in the Main Menu.The "Favorite Programs" list will appear.2. Highlight the selection by pressing it.Note: Use the scroll bar to scroll through the list quickly.Note: Press Select by Number to search for the program by number. Use the alphanumeric keypad to enter the test number (program number) and confirm the input by pressing OK.3. Press Remove program.
93Advanced operations4. Press OK to confirm.Note: If a Favorite Programs is deleted, it will stay in the User Programs or Stored Programs.Note: If the stored program is deleted in User Programs, it will also be deleted in Favorites Programs.6.3 Standard Addition - monitoring/checking resultsThe accuracy of readings (their correspondence with the actual concentration of the analyte in the sample) and their precision (correspondence of the reading results obtained from several samples containing the same concentration of the test analyte) can be determined or improved using the standard addition methodThis method (also known as standard-addition or spiking) serves to detect sample-specific faults: •The analysis was inaccurate by substances in the sample (sample matrix effect), •defective measuring device, •impure reagents.Method:Add a defined quantity (concentration) of the substance to be tested to the sample as a standard solution. The detection rate (concentration of the added standard solution) should be close to 100%.Detection rate =Reading of successful standard additionExpanded value after successful standard additionDetection rate Conclusion100% Probability that the reading results are correct is high. 100%Assumption: The analysis was inaccurate by substances in the sample (sample matrix effect)Test to determine whether a sample matrix effect is present:Use distilled water instead of the sample. Add standard solution as described in the procedure.Detection rate Conclusion100%Ions in the sample are interfering with the analysis, causing false results to be obtained. 100% No interference ions.
94Advanced operationsMeasures to identify other interference factors:Checklist:1. Check if the procedure is completed correctly:a. Are the reagents added in the correct order?b. Is enough time allowed for colour development?c. Is the correct glassware in use?d. Is the glassware clean?e. Does the test require the sample to be at a certain temperature?f. Was the pH of the sample in the correct range?g. Is the pipette volume correct?2. Check the reagents. Repeat the standard additions using fresh reagents.If none of these measures resolves the problem, please contact the manufacturer.6.3.1 Complete a standard additionCarry out the standard addition in accordance with the corresponding procedure.There are two different methods:Maximum Volume (standard addition)A defined volume of a standard solution of known concentration is incrementally added to a previously tested sample and the sample is newly measured after every standard addition.Sample volume:A defined volume of a standard solution of known concentration is added to the volume of sample specified in the procedure and the sample is measured after each addition. In most cases three different standard solutions are prepared and the procedure is repeated for each of them.Note: The units and chemical forms used for the sample are used for the standard solutions. Be careful to make sure that to use the correct units for subsequent entries.Detection rate Conclusion100%The originally used reagents were defective. Check the standard solution: Repeat the standard addition procedure with a freshly prepared standard solution and distilled water.Detection rate Conclusion100 %The originally used standard solution was deficient.
95Advanced operationsMaximum Method Volume/Sample volume1. Press Stored Programs in the Main Menu. 2. Select the required program.3. Press Start.4. Analyze a sample without added standard solution in accordance with the instructions in the Procedures Manual. When the reading is complete, leave the sample cuvette/cell in the cuvette/sample cell compartment.5. Press Options>More>Standard Addition.An overview of the data of the standard addition procedure is displayed.6. Press OK to accept the standard values for standard concentration, sample volume (total) and standard addition volume. 7. Press Edit, to change these values.8. Press the key of the value to change it. Use the alphanumeric keypad to change the value. 9. Press OK to confirm.
96Advanced operations10. Press the keys to change the standard addition volumes. Use the alphanumeric keypad to enter the new data.11. Press OK to confirm.Description of the table of readings•The first column shows the standard addition volume. 0 mL stands for a sample containing no added standard.•The second column shows the reading of the samples with and without added standard.•The third column shows the detection rate of the added standard.Note: The black highlighted line is active.The reading of the sample in the instrument, without added standard, is automatically shown under 0 ml.12. Prepare the standard addition solution as described in the procedure.13. Press the standard addition volume in the table.14. Insert the cell with the respective addition volume into the cell compartment. 15. Press Read. Repeat the procedure with all the other standard addition solutions.16. After all the standard addition solutions have been measured, press Graph.The regression line through the standard addition data points is displayed. The correlation coefficient r2 indicates how close the data points are to the line.If the correlation coefficient = 1, the curve is linear.The concentration shown above the curve is the estimated concentration of the sample without the added standard.Note: In the Graph menu, the name on the Graph key switches to Table. Press Table to display all the data in the table again.17. Press Ideal line to display the relationship between the added standard solutions and the ideal line (detection rate 100%).
97Advanced operations6.4 Single Wavelength (absorbance, concentration and transmittance readings)The Single Wavelength mode can be used in three ways. For sample readings at a single wavelength, the instrument can be programmed to measure the absorbance, % transmittance or concentration of the analyte.•Absorbance readings: The light absorbed by the sample is measured in absorbance units.•% Transmittance measures the percent of the original light that passes through the sample and reaches the detector.•Turning on the concentration factor allows selection of a specific multiplier for converting absorbance readings to concentration. In a graph of concentration versus the absorbance, the concentration factor is the slope of the line.6.4.1 Set up Single Wavelength mode1. Press Single Wavelength in the Main Menu. 2. Press Options for Parameter Setup.Table 12 Options for Single Wavelength Options DescriptionMore For further OptionsRecall measurement data symbol Recall saved measurement data, wavelength scans or time courses, refer to Section 5.3, page 43.% Trans/Abs To switch to % transmittance, concentration or absorbance readingsWavelength To input the wavelength setting. Use the alphanumeric keypad to enter the reading wavelengths. The entered wavelength must be in the range from 320-1100 nm.Timer iconThis functions as a stopwatch. It helps to make sure that the steps of an analysis are correctly timed (e. g. reaction times, wait times, etc., can be exactly specified). When the specified time has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the reading program.Concentration Factor Multiplication factor for converting absorbance values into concentration values.Concentration Resolution To select the number of decimal places.
98Advanced operationsConcentration Factor:1. Press Concentration Factor: Off in the "Options" menu. 2. Press On to highlight this feature.3. Press Factor and enter the factor. 4. Press Unit and select the corresponding units for concentration from the displayed list.5. Press OK to confirm.Concentration Resolution:1. Press Concentration Resolution in the "Options" menu.2. Select the resolution.3. Press OK to confirm.4. Next press Return to return to the results screen.Save as User Program To store the selected parameters as a User Program, refer to Section 6.1, page 73.Recall measurement data Recall saved measurement data, wavelength scans or time courses, refer to Section 5.3, page 43.Instrument Setup mode Basic data of the instrument, refer to Section 5.2, page 27.Table 12 Options for Single Wavelength(Continued)Options Description
99Advanced operations6.4.2 Take single wavelength readings (single reading)1. Insert the zero solution cell into the cell compartment.2. Press Zero.Note: The Read key is only active after the zero reading has been completed.3. Insert the analysis cell into the cell compartment.4. Press Read. 5. For data storage, refer to Section 5.3.1, page 43.6.5 Multi Wavelength mode – readings with more than one wavelengthIn the Multi Wavelength mode, absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums, differences and relationships.•Absorbance readings: The light absorbed by the sample is measured in absorbance units.•% Transmittance measures the percent of the original light that passes through the sample and reaches the detector.•Turning on the concentration factor allows selection of a specific multiplier for converting absorbance readings to concentration. In a graph of concentration versus the absorbance, the concentration factor is the slope of the line. Concentration is calculated using a single factor for each wavelength, which is input by the user.6.5.1 Set the Reading mode at different wavelengths Press Multi Wavelength in the Main Menu. Press Options for Parameter Setup.Table 13 Multi wavelength setup options Options DescriptionMore For further OptionsRecall measurement data symbol Recall saved measurement data, wavelength scans or time courses, refer to Section 5.3, page 43.% Trans/Abs To switch to % transmittance, concentration or absorbance readings
100Advanced operationsAbsorbance Formula:1. Press Absorbance Formula. 2. The formula selected in the top key determines the number of wavelength and coefficient keys that will appear below. 3. Press the key to change the calculation formula.4. Select a formula from the displayed list.5. Press OK. When a new formula is selected, the number of variables below changes to match.The following formulas are available:K 1 A 1 + K 2 A 2K 1 A 1 + K 2 A 2 + K 3 A 3K 1 A 1 + K 2 A 2 + K 3 A 3 + K 4 A 4K 1 A 1 / K 2 A 2(K 1 A 1 + K 2 A 2 ) / K 3 A 3(K 1 A 1 + K 2 A 2 ) / (K 3 A 3 + K 4 A 4 )Wavelength To input the wavelength setting. Use the alphanumeric keypad to enter the reading wavelengths. The entered wavelength must be in the range from 320–1100 nm.Timer iconThis functions as a stopwatch. It helps to make sure that the steps of an analysis are correctly timed (e. g. reaction times, wait times, etc., can be exactly specified). When the specified time has elapsed, an acoustic signal is emitted. The use of the timer has no influence on the reading program.Concentration Factor Multiplication factor for converting absorbance values into concentration values.Concentration Resolution To select the number of decimal places.Absorbance Formula Calculation basis for evaluating samplesSave as User Program To store the selected parameters as a User Program, refer to Section 6.1, page 73.Recall measurement data Recall saved measurement data, wave-length scans or time courses, see Section 5.3, page 43.Instrument Setup mode Basic data of the instrument, refer to Section 5.2, page 27.Table 13 Multi wavelength setup options(Continued)Options Description
101Advanced operationsA 1 refers to the absorbance at wavelength 1A 2 refers to the absorbance at wavelength 2, etc. K 1 refers to the coefficient at wavelength 1K 2 refers to the coefficient at wavelength 2, etc. If a subtraction has to be completed, the factors can be entered with a minus sign.6. Press the "x" keys to change the wavelengths. 7. Enter the required wavelength factor using the alphanumeric keypad.8. Press OK to confirm the selection.9. To change a coefficient, press one of the "KX" keys. 10. Enter the required coefficient using the alphanumeric keypad.11. Press OK to confirm the selection.Note: Up to 5 digit number values can be entered! A maximum of four decimals places are possible.Concentration Factor:1. Press Concentration Factor: Off in the "Options" menu. 2. Press On to highlight this feature.3. Press Factor.4. Enter the factor with which the absorbance measurement is to be multiplied. 5. Press Unit. 6. Select the respective concentration unit from the displayed list.7. Press OK to confirm.
102Advanced operationsConcentration Resolution:1. Press Concentration Resolution in the "Options" menu.2. Select the resolution.3. Press OK to confirm.4. Press Return to return to the results screen.6.5.2 Complete a reading in the Multi Wavelength mode1. Insert the zero solution cell into the cell compartment.2. Press Zero. Note: The Read key does not become active until the zero reading has been completed.3. Insert the analysis cell into the cell compartment. 4. Press Read. 5. For data storage, refer to Section 5.3.1, page 43.6.6 Wavelength Scan mode – recording of absorbance and transmission spectrumsIn the Wavelength Scan mode, the absorbance of the light in a solution over a defined wavelength spectrum is measured. While the scan is taking place, the cell compartment cover must be closed. Therefore, a scan of the round cell compartment is not possible.The reading results can be displayed as a curve, as percentage transmittance (%T) or as Absorbance (Abs). The collected data can be printed as a table or a curve.The data is available for formatting changes. These include automatic scaling and zoom functions. Maximum and minimum values are determined and shown as a table.The cursor can be moved to any point on the curve for the purpose of reading off the absorbance or transmittance value and the wavelength. The data associated with each data point can also be shown as a table.
103Advanced operations6.6.1 Set up the wavelength scanPress Wavelength Scan in the Main Menu. Press Options for Parameter Setup.Setting of the wavelength1. Press the key in the "Options" menu to select the wavelength range and the wavelength step.2. Press the upper left key to select the lower wavelength. 3. Enter the lower wavelength.4. Press OK to confirm.5. Press the upper right key to select the upper wavelength. Table 14 Options during wavelength scan Option DescriptionMore Further options are displayedFolder icon To store the scan dataReference Off/OnFrom the displayed list of stored scans, a record is selected for use as a reference scan/superimposed scan. This can be highlighted or shown in the background in comparison with the actual measured scan.Note: This option is only available when there are stored scans with the same wavelength range and step. To enter the wavelength spectrum and the scan intervalSelect View Enables the user to switch the display back and forth between the scan data tables (wavelength/absorbance) and the graph of the curve.Note: "Select View" will be activated after the first reading.Cursor Mode To select Track or Peak/Valley. The selection for this menu item determines to which points on the graph the cursor moves.Send Data To send data to a printer, computer or USB memory stick (Type A)Integral: On/Off The integral gives the area and the derivative of the integral gives the original functionScale & UnitsScale: In the automatic Scaling mode, the y-axis is automatically adjusted so that the total scan is displayed. The manual Scaling mode allows sections of the scan to be displayed. Units: Choice of absorbance or transmittance.Recall measurement data Recall saved measurement data, wave-length scans or time courses, see Section 5.3, page 43.Instrument Setup mode Basic data of the instrument, refer to Section 5.2, page 27.
104Advanced operations6. Enter the upper wavelength.7. Press OK to confirm.Note: Do not select the same wavelength for minimum and maximum.8. Highlight the required wavelength step. Note: The selection of the interval influences the duration and resolution of the Wavelength Scan. Scans with a high resolution take longer than scans with a low resolution. The larger the increments of the intervals selected, the faster the scan runs. In contrast, the resolution of the determined data decreases. In summery maximally 780 measuring steps can be accomplished during a Scan.Note: The maximum wavelength adjust automatically if the difference between the maximum and minimum wavelength is not a multiple of the interval.9. Press OK to return to the Scan mode. Selected parameters are displayed along the graph‘s x-axis.Select view (display table)1. Perform a Wavelength Scan (Section 6.6.2, page 105)2. Press Display table in the "Options" menu.3. A table with the results is displayed.4. To return to the graph press Options and then View Graph.Cursor Mode1. Press Cursor mode: tracking in the "Options" menu.The selection for this menu item determines what data is displayed in the table. 2. Highlight Track or Peak/Valley.3. Press OK to confirm.4. Press Return to return to the Scan mode.IntegralThe Integral applies to the whole wavelength range of the scan.1. Press Integral: Off in the "Options" menu.2. Highlight On to show the Integral. 3. To find the integral of other wavelength ranges, change the wavelength range and scan again.4. Press OK to confirm.
105Advanced operations5. Press Return to return to the Scan mode.Note: The Integral is shown instead of the date on the display.Note: For the next scan reading the setting for the Integral will be On.Scale & Units1. Press Options>More..>Scale & Units.2. Highlight the required units (Abs or %T).3. Highlight Auto or Manual scaling on the graph's y-axis. Note: When selecting the manual scaling, the limit values y min and y max can be entered using the alphanumeric keypad. The graph is adjusted to display only the values in the selected range. If automatic scaling is selected, the instrument sets the limits automatically so that the total range can be displayed.4. Press OK.5. Press Return to return to the Scan mode.6.6.2 Wavelength scan readingAfter selecting all scan parameters, record a base line (initial zero reading). If a scan parameter is changed, a new base line must be recorded. When the baseline has been scanned, the instrument is ready to scan one or more samples.1. Press Wavelength Scan in the Main Menu.2. Insert the zero solution cell into the cell compartment and close the cell compartment cover.Note: The cell compartment must be closed via the cell compartment cover during a scan. Therefore, no scans can be performed with 13-mm round cells.3. Press Zero. "Zeroing" is shown below the graph as the baseline scan begins.4. Insert the prepared analysis cell into the cell compartment and close the cell compartment cover.5. Press Read. Under the graph, the display "Reading..." appears, and a graph of the absorbance or transmission values for the scanned wavelengths is continuously displayed.
106Advanced operationsThe Wavelength Scan is complete, if•the graph is shown full-size,•the scaling of the x-axis occurs automatically,•the Cursor functions in the vertical navigation bar are highlighted.•a signal sounds6.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis6.6.3 Work with reference scansThere are two options to work with Reference Scan:First option:1. Press Reference: Off in the "Options" menu to select another scan to display on the same screen with the current scan. Highlight the required scan number and press Highlight reference.Note: After selecting a reference scan the Reference: Off key in the "Options" menu turns into Reference: On.Note: Only scans that have the same wavelength range and step can be displayed using the overlay option. This process can be repeated until all matching scans are displayed.2. The reference curve is shown in orange. The absorbance or transmittance value and the associated wavelength are highlighted in grey.Note: A black and an orange box are shown in the top left corner of the display. The orange box relates to the reference scan and the black one relates to the current wavelength scan. Table 15 Navigating the wavelength scanCursor function/Zoom function DescriptionCurve Icon(Choice of Cursor mode) Choice of Cursor mode Peak/Valley (cursor moves between minimum/maximum absorbance values) or Cursor mode Tracking (cursor moves over each data point of the scan).Arrow keysThe arrow keys (right/left) are used to move the cursor (depending on the selected mode) to the next data point. The data of the data point (wavelength/absorbance or transmittance value) are highlighted on the x and y axes. Note: Press any point on the curve to display the associated data.Zoom Icon This function is used to magnify the section of the curve in the vicinity of the cursor. The original curve size can be restored by pressing the zoom icon again.
107Advanced operations3. To complete the wavelength scan reading, refer to Section 6.6.2, page 105.•The newly plotted wavelength scan curves are shown in black.•The absorbance or transmittance value and the associated wavelength are highlighted in black.•In addition, the display shows the difference between the wavelength scan curve and the reference curve against the wavelength.4. Press the black or orange small box in the left upper corner on the screen to switch between the actual wavelength scan and reference scan.Second option:1. Insert the zero solution cell into the cell compartment. Press Zero.2. Insert the analysis cell into the cell compartment. Press Read.•The newly plotted wavelength scan curves are shown in black.•The absorbance or transmittance value and the associated wavelength are highlighted in black.3. Press Options and then Reference: Off in the "Options" menu to select another scan to display on the same screen with the current scan. Highlight the required scan number and press Highlight Reference.Note: After selecting a reference scan the Reference: Off key in the "Options" menu turns into Reference: On.Note: Only scans that have the same wavelength range and step can be displayed using the overlay option. This process can be repeated until all matching scans are displayed.4. The reference curve is shown in orange. The absorbance or transmittance value and the associated wavelength are highlighted in orange.•In addition, the difference of the absorbance and/or transmission value between the two scans (measured scan and reference scan) is indicated/highlighted at each position of the cursor.Note: A black and an orange box are shown in the left corner of the display. The orange box relates to the reference scan and the black one relates to the current wavelength scan. 5. Press the black or orange small box in the left upper corner on the screen to switch between the actual wavelength scan and reference scan.
108Advanced operations6.7 Time course of absorbance/transmittanceThe Time Course mode is used to collect data in either absorbance or transmittance for a user-specified length of time. This data can be displayed in either graphic or tabular format. While the time scan is taking place, the cell compartment cover must be closed. Therefore, a scan of the round cell compartment is not possible.6.7.1 Time course setup parameters1. Press Time Course mode in the Main Menu. 2. Press Options to configure parameters.Table 16 Time course options Options DescriptionMore For further optionsFolder icon To store the scan dataTime & Interval To input the total time for data collection and the time interval between the collection of data pointsTo input the wavelength settingView Table To display readings in absorbance, transmittance or concentration. This can be changed after sample data is collectedScale & UnitsScale: In the automatic Scaling mode, the y-axis is automatically adjusted so that the total scan is displayed. The manual Scaling mode allows sections of the scan to be displayed. Units: Choice of absorbance or transmittance.Send Data To send Data to a printer, computer or USB memory stick (Type A)Recall measurement data Recall saved measurement data, wave-length scans or time courses, see Section 5.3, page 43.Instrument Setup Basic data of the instrument, refer to Section 5.2, page 27.
109Advanced operationsTime & Interval:1. Press Time & Interval in the "Options" menu.2. Enter the total time and measurement interval.3. Press OK to confirm.Note: In total, 500 measuring steps can be accomplished during a scan. To select a total time and a time interval that would cause this number of readings to be exceeded, the time interval is defined automatically and the OK key is inactivated.Scale & Units:1. Press Scale & Units in the "Options" menu.2. Highlight Abs or %T as the required units.3. Highlight Auto or Manual scaling on the graph's y-axis. Note: When selecting the manual scaling, the limit values y min and y max can be entered using the alphanumeric keypad. The graph is adjusted to display only the values in the selected range. If automatic scaling is selected, the instrument sets the limits automatically so that the total range can be displayed.4. Press OK to confirm.5. Press Return to return to the Scan mode.6.7.2 Time course scan readingAfter all scan parameters have been selected, the instrument must then be zeroed. Afterward the sample can be analyzed.1. Insert the zero solution cell in the cell compartment and close the cell compartment cover. 2. Press Zero. The blank reading is shown on the display.3. Insert the analysis cell in the cell compartment and close the cell compartment cover. 4. Press Read. The instrument will begin collecting Time Course data (kinetic data).Note: During the reading the Zero and Read keys change to Mark and Stop.
110Advanced operations• Select Mark to mark the next data point collected. This mark is not used by the instrument, but is available for the user and may indicate a significant event, such as the addition of a sample or other reagent. The mark is also shown in the table.•Select Stop to stop taking sample readings.6.7.3 Analysis of time course dataAfter the Time Course data has been collected, the following manipulations can be done on the graphic data:The Time Course Program is complete, if •the instruments beeps after completion of the overall time•the graph is shown full-size,•the scaling of the x-axis occurs automatically,•the Cursor functions in the vertical navigation bar are highlighted.6.7.3.1 Navigation of a time scan or a time scan analysisAfter a time scan has been completed, the time and the absorbance/transmittance data is displayed as a curve.Where the cursor is positioned on the curve, the elapsed time up to this point and the corresponding absorbance are highlighted.Table 17 Navigating the time scanCursor function/Zoom function DescriptionCurve Icon(Choice of Cursor Mode)Delta mode: A second cursor is highlighted. The position of the fixed cursor was previously defined in Cursor mode Single. Use the active cursor to select any point on the reading curve. The difference to the fixed cursor is shown on the curve. The delta values are correspondingly highlighted and displayed on the x and y axes.The gradient of the curve and the correlation coefficient (r2) between the cursor points in the Delta mode are shown under the curve.Cursor mode, single: The cursor moves to each selected measuring point of the scan.Arrow keysThe arrow keys (right/left) are used to move the cursor (depending on the selected mode) to the next data point. The data of the data point (wavelength, absorbance or transmittance value) are highlighted on the x and y axes.Note: Press any point on the curve to display the associated data.Zoom Icon This function is used to magnify the section of the curve in the vicinity of the cursor. The original curve size can be restored by pressing the zoom icon again.
111Advanced operations6.8 System Checks1. Press System Checks in the Main Menu.The "System Check" menu contains instrument information and various performance tests.6.8.1 Instrument Information1. Press Instrument Information in the "System Check" menu.2. The model, serial number and software version are displayed.6.8.2 Update the instrument softwareTo obtain the software for the update from the Internet at www.hach.com.1. Go to http://www.hach.com.2. Select download resources>software downloads.3. Enter DR 3900 in "Search for documents".4. Locate the appropriate download file.5. Open the ZIP file and save the files to the USB memory stick or PC. The files have to be unzipped prior to saving on USB or PC.6. Press Instrument Update in the "System Check" menu.
112Advanced operations7. Connect the USB memory stick to the USB port (type A) on the instrument, refer to Section 3.5, page 18.8. Press OK to confirm. The link is established automatically and the software is updated.9. Press OK to return to the "System Check" menu.Note: After the instrument update, a prompt to restart the instrument will be displayed.6.8.3 Optical Checks1. Press Optical Checks in the "System Check" menu.The "Optical Checks" menu contains programs for checking the wavelength accuracy, stray light and photometric accuracy. An optional test filter set (Verification Kit) Section 9, page 137, containing six precision glass filters, target values and instructions is available as an aid for carrying out comprehensive in-house instrument checks.6.8.3.1 Verification kitThe Verification Kit (refer to Section 9, page 137) is designed for periodic monitoring of scattered light, photometric accuracy and the wavelength accuracy of the spectrophotometers. When results exceed allowable tolerances (given in the quality control certificate to the test record), contact the manufacturer.1. Press Verification Kit in the "Optical Checks" menu.2. Press Nominal Values.3. Press Edit.An automatic menu guidance queries values (filters, wavelength, nominal values and tolerances) given in the quality control certificate, to the following specifications:•Stray light•Photometrical accuracy•Wavelength accuracy4. Press OK when all values are entered and the overview is displayed.
113Advanced operations5. Press Verification.6. Insert cell adapter A (Figure 3, page 19) in cell compartment (2).7. Remove any remaining cells from the cell compartment.8. Close the cell compartment cover.9. Press Start.10. Follow the directions on the display and set various filters one after another in the order displayed. 11. Press Next after inserting a filter.After the last reading the results are displayed.12. Press PC & Printer icon to send the data to a USB memory stick, PC or to a printer.The files will be stored automatically as CSV files (Comma Separated Value). The file name will be formatted as "Verification.csv".6.8.3.2 Wavelength checkThe Wavelength Check test is used to check wavelength accuracy at 807 nm.
114Advanced operations1. Press Wavelength Check in the "Optical Checks" menu.2. Insert the cell adapter (A) for 10-mm rectangular cells in the cell compartment (2).3. Place the analysis cell (Neodym or BG20/2) in the cell adapter. 4. Close the cell compartment cover. 5. Press Start. The result will be displayed.6. Compare the result with the analysis cell required values.7. Press Cancel to return to Optical Checks.
115Advanced operations6.8.3.3 Stray light checkThe Stray Light test is used to measure the stray light in the instrument at 340 nm.1. Press Stray Light Check in the "Optical Checks" menu.2. Remove any cuvettes/analysis cuvettes from the cell compartment.3. Place the cell adapter (A) for 10-mm rectangular cells in the cell compartment (2). 4. Close the cell compartment cover.5. Press Zero.6. Insert the analysis cell into the cell compartment (2).7. Close the cell compartment cover.8. Press Start.The mean value is calculated from three consecutive absorbance measurements. The result will be displayed.9. Compare the result with the required value from the analysis cell.10. Press Cancel to return to Optical Checks.6.8.3.4 Absorbance checkThe Absorbance Check test is used to test the photometric accuracy and repeatability of the instrument.1. Press Absorbance Check in the "Optical Checks" menu.2. Press  to input the wavelength.3. Press OK to confirm.4. Remove any cells from the cell compartment.5. Close the cell compartment cover.6. Press Zero.
116Advanced operations7. Place the analysis cell in the cell compartment.8. Close the cell compartment cover.9. Press Read. 10. Remove the analysis cell.11. Close the cell compartment cover.12. Press Zero.13. Place the analysis cell in the cell compartment.14. Close the cell compartment cover.15. Press Read. 16. Repeat this procedure until 5 comparison measurements have been obtained.The result will be displayed.17. Compare the result with the required value from the analysis cell.18. Press Cancel to return to Optical Checks.6.8.4 AQA - Analytical Quality Assurance1. Press AQA in the System Checks menu to configure AQA measures or AQA in the toolbar to the right of the display, to carry out AQA measures.The AQA menu in System Checks contains the program for checking the analytical quality. Configuration is done here, for example the individual working method being controlled by standard solutions or the cross-sensitivities of the analyses identified by standard addition solutions, multiple determinations and dilutions. As optional accessories for full individual control, standard and top-up solutions are available (Section 9, page 137).
117Advanced operations6.8.4.1 AQA Standard ConfigurationThe working method, photometers, and accessories can be checked by the analysis of a standard solution.1. Press AQA StandardA bar with standard sets opens (not available on all models), alternatively an individual standard can be defined.2. Select Options>New to define a new standard. Note: If a standard has not yet been defined, in some models the alphanumeric keypad is displayed directly.3. Enter the name of the standard solution and confirm with OK.4. Enter the concentration unit and confidence interval by touching the corresponding fields. 5. Press OK to confirm the entry.6. All the standard solutions entered are shown in the bar.7. Select Options>Edit to alter the settings.8. Select Options>Delete to delete the standard. Select Options>New to define a new standard.
118Advanced operations9. Select Options>Reminder to enter an automatic interval when a standard analysis is required to be carried out.10. Press On.11. Select the required interval. Press OK to confirm the entry.12. Select a unit for the interval. Press OK to confirm the entry.6.8.4.2 AQA AdditionBy the analysis of a addition solution it is possible to check for faults and interferences in the sample1. Press AQA Addition.2. Press On.
119Advanced operations3. Enter the required interval, and the system will automatically issue a prompt to add an analysis. Press OK to confirm the entry.4. Define the required unit for the interval. Press OK to confirm the entry.6.8.4.3 Multiple Determination1. Press Multiple Determination.2. Press Reminder <Off>.3. Press On.4. Select the required interval. Press OK to confirm the entry.5. Select a unit for the interval. Press OK to confirm the entry.6. Enter the number of measurements for multiple definitions. Press OK to confirm.7. Enter the tolerance as a percentage. Press OK to confirm.8. Press OK to confirm all the entries.
120Advanced operations6.8.4.4 Dilution1. Press Dilution.2. Press On.3. Select the required interval. Press OK to confirm the entry.4. Select a unit for the interval. Press OK to confirm the entry.5. Enter the dilution factor. Press OK to confirm.6. Enter the tolerance as a percentage. Press OK to confirm.7. Press OK to confirm all the entries.6.8.4.5 Carring out AQA measuresIf a reminder function has been set, once the set interval has elapsed the requirement to carry out the AQA measure is issued.1. Select the required AQA measure – for example a standard measurement here.2. Carry out the standard measurement via stored program or barcode program.3. An AQA symbol is displayed in the measurement window, the AQA key in the toolbar is displayed as yellow.4. The AQA measure is shown in a log as Passed or Failed.6.8.4.6 Pipette test setThis function and the optional accessories tests the accuracy of the pipette test set. To carry it out follow the instructions in the pipette test set.
121Advanced operations6.8.5 Instrument backupPrior to the next service date, the "Instrument Backup" offers the opportunity to store all programs, measurement data, user IDs, passwords, as well as all adjustable data on a USB stick.1. Press Instrument Backup in the "System Check" menu.2. Insert the USB stick (refer to Section 3.5, page 18).3. Press Store to start a Backup. If the file was stored, the message "Instrument Backup is stored" will be displayed.4. Press OK to return to the "System Check" menu.Restore backup data:Note: All current data will be overwritten when restoring the Backup file!1. Press Instrument Backup in the "System Check" menu.2. Insert the USB stick with the backup (refer to Section 3.5, page 18).3. Press Restore to pass back the data.4. The report "Restore instrument backup from S/N XXXXXXX. ?" displays the version of the backup. Press OK to confirm.5. After the backup start the instrument again.
122Advanced operations6.8.6 Service menuUse of the service menu requires a service code. This menu is reserved exclusively for servicing tasks.6.8.7 Service timeIn order to make sure a regular inspection is performed, an automatic memory reference for the service times can be entered. After switching the instrument on, this memory reference will be activated and indicated at the appropriate time.1. Press Service Time in the "System Check" menu.2. Activate the On checkbox.3. Press Last service.4. Enter the date of the last service.5. Press OK to confirm the selection.6. Activate the options field under Next service.7. Select a time interval for the next service.8. Press OK to confirm the selection.When the next service is due the report "Next service due!" is displayed when the instrument is switched on.9. Press OK to return to the Main Menu.Contact the manufacturer to agree a date for the next service.
123Advanced operations6.8.8 Lamps operating timeThe "Lamps operating time" provides information on the switch-on cycles of the lamps.After a lamp is replaced, the display of the switch-on cycles is reset to 0.1. Press Lamp History in the "System Check" menu.2. Press Reset and the lamp data are reset.3. Press OK to return to the "System Check" menu.6.9 ToolbarOn the right of the display there is a toolbar with various programs. Many of these programs can also be called up via a menu but the operation is the same.6.9.1 Log onUse this function to enter up to 30 different operator initials (each with a maximum of 10 characters) into the instrument. This feature helps record which operator measured each sample. For a detailed description, refer to Section 5.2, page 27.
124Advanced operations6.9.2 Sample IDUse this option to enter up to 100 different Sample Identification tags (with up to 20 characters each) into the instrument. Sample IDs can be used to specify the sample location or other sample specific information, for example. For a detailed description, refer to Section 5.2.2, page 29.6.9.3 TimerUse this function to activate a timer at any time.1. In the toolbar, press Timer.2. Enter the required time and confirm with OK.The count-down starts automatically.3. Press Close to let the timer run in the background.Press Cancel to stop the timer.4. When the timer has finished there is an acoustic signal.6.9.4 AQAThe "AQA" menu contains the programs for testing the analytical quality. For example, this controls the individual working method with standard solutions or the cross-sensitivities of the analyses identified by addition solutions, multiple determinations and dilutions. For a detailed description, refer to Section 6.8.4, page 117.
125Advanced operations6.9.5 TrendsUse this function to call up a load curve of the stored measurement values for each parameter or sample location. The concentration of the corresponding parameter is shown graphically over time. For a detailed description, refer to Section 5.3.4.1, page 51.6.9.6 Link2SCLink2SC enables measurement values to be exchanged between on-line process probes and the DR 3900. In this way on-line data can be verified and process probes can be corrected.The on-line data are sent to the DR 3900 in a "job file". After the laboratory measurement values have been produced, the job file is sent to the controller again for the corresponding probe to be corrected.Data transfer is either via memory card or network (LAN). For a detailed description, refer to the Link2SC operating instructions.6.9.7 Website DR 3900Use this function to call up an Internet website on an existing network connection. Current software updates, as well as documentation and information on DR 3900 can be found here. The labelling of the key varies from model to model and may vary from the illustration in these instructions.
126Advanced operations
127Section 7 Maintenance7.1 Cleaning requirements7.1.1 Spectrophotometer•Only clean the housing, the cell compartments and all accessories with a soft, damp cloth. A mild soap solution can also be used. •Do not get excess water in the cell compartments. •Do not insert a brush or sharp object into cell compartment #1 to avoid damaging the mechanical components.•Dry the cleaned parts carefully with a soft cotton cloth.7.1.2 Display•Do not scratch the display. Do not touch the screen with ball pens, pencils or similar pointed objects.•Clean the display with a soft, lint-free and oil-free cotton cloth. Diluted window cleaner liquid can also be used.CAUTIONPotential Chemical, Biological Eye and Skin Hazards.Only qualified personnel should conduct the tasks described in this section of the manual.NOTICERemove all cells remaining in the instrument. Dispose of the cells or their contents in an authorised manner.CAUTIONPotential Pinch, Eye, Burn and Chemical Hazards.Before cleaning, always disconnect the instrument from the power supply.NOTICENever use solvents like turpentine, acetone or similar to clean the instrument, including the display and accessories.
128Maintenance7.1.3 Cuvettes/cells1. After use, clean the glass cells with cleaning agents.2. Afterwards, rinse the cuvettes/sample cells several times with tap water and then thoroughly with deionized water.Note: Glass cuvettes/sample cells that have been used for organic solvents (such as chloroform, benzene, toluene, etc.) must be rinsed with acetone before being treated with cleaning agents. In addition, another rinse with acetone is necessary as a final treatment step before the cuvettes/sample cells are dried.7.2 Lamp replacement1. Switch off instrument.2. Remove the power cable from the instrument.3. Turn the instrument to allow work access to the underside.4. Using a slot-head screwdriver, undo the lamp cover screws.5. Remove the cover.CAUTIONPotential Chemical/ Biological Exposure Hazards.User correct laboratory practices if a risk of chemical exposure exists.CAUTIONTo avoid a possible electric shock, disconnect the instrument from the power source before servicing the lamp. NOTICEBurn Hazard! Wait until the lamp cools down. Contact with the hot lamp can cause burns.
129Maintenance6. Using a cross-head screwdriver, remove the two screws for the lamp holder.7. Lift out the lamp holder upwards.8. Turn the lamp holder to that the connector with the cable guide points forwards.9. Push the clamp slide as far as possible to the right (Step 1). 10. Grasp the connector and along with the lamp, pull it out of the lamp holder (Step 2).11. Release the connector from the lamp (Step 3).
130MaintenanceFig. 8 Lamp replacement To fit a new lamp carry out the procedure in reverse.1. Connect a new lamp with the connector.2. Replace the lamp along with the connector into the lamp holder.3. Secure the lamp with the clamp slide. To do this, push the connector downwards and push the clamp slide as far as possible to the left.4. Replace the lamp holder into the instrument.Note: Take care that the lamp cable is not pinched by the cable guide.5. Using a cross-head screwdriver, tighten the two lamp holder screws.6. Replace the lamp cover.7. Using a slot-head screwdriver tighten the cover screws.8. Connect the power cable to the instrument.9. Reset the lamp operating time Section 6.8.8, page 124.1Lamp holder 3Lamp2Clamp slide 4ConnectorNOTICEOnly hold lamp at lamp holder. Avoid touching the glass, as substances on the skin can bake onto the lamp bulb and thus accelerate the ageing process of the lamp.
131Maintenance7.3 Exchange of the replaceable cell compartmentIf there is severe soiling, the complete cell compartment (2) can be replaced.1. Switch off instrument.2. Remove the power cable from the instrument.3. Using a cross-head screwdriver, undo the two screws on the floor of the cell compartment.4. Lift the cell receptacle upwards and out.5. Emplace the new cell receptacle so that the pressure rollers guide slot is to the left.6. Using a cross-head screwdriver, tighten the two screws on the floor of the cell compartment.7. Connect the power cable to the instrument.
132Maintenance
133Section 8 TroubleshootingError displayed Definition ResolutionAttention!Please insert the light shield. Readings with barcode cuvettes generally require the light shield.Insert the light shield.Press OK.Barcode label not read Barcode faultyReinsert the cell.If the barcode is not recognised, contact the manufacturer or his representative. An error occurred when uploading the instrument data. Start the procedure again or contact the manufacturer or his representative.An error occurred when reading from the USB memory stick. Start the procedure again or contact the manufacturer or his representative.An error occurred when writing to the USB memory stick. Start the procedure again or contact the manufacturer or his representative.Please check on the current update file. Error during update. Check the USB memory stick. Please contact Customer Services. Error during update.Please check network configuration.Please check the connection.Please close the cover. Close the cover.Please insert the USB memory stick. Insert a USB memory stick into a USB A port on the instrument.Please check the connection and contact the administrator. Network setup or FTP errorBlank value correction not possible! Blank value correction not possible with LCW919.File for instrument update missing. Error during update. Check the USB memory stick. File for instrument update is faulty. Error during update. Save the update file again and repeat the procedure.It’s recommended to execute a Full System Check Check of the air values failedSwitch the instrument of and then back on again. If the system check is not successful, contact the manufacturer or his representative.Entry invalid! Password incorrectForgotten your password?Contact the manufacturer or his representative.Absorbance > 3.5! The measured absorbance exceeds 3.5 Dilute sample and measure againFaultBarcode check number?Please update program data! Deviation to the stored data Data updatingError when calling up the local IP address. Network setup: DHCP client has no connection to the DHCP server Enter the IP address again.Error during default gateway setup. Network setup: default gateway cannot be set for fixed IP address Try to create the connection again.Error during network drive setup! Error during network setup Check the settings.Error during subnet mask setup. Network setup: Subnet mask cannot be set for fixed IP address Enter the subnet mask again.
134TroubleshootingError copying from USB memory stick. Error during update Start the procedure again or contact the manufacturer or his representative.Error in FTP connection. FTP error Make sure that the instrument is connected to the network. FaultProgram not accessible.Please update program data! Barcode test not present Data updatingFaultClean cuvette! The cuvette is soiled or there are undissolved particles in the cuvetteClean the cuvette; allow the particles to settleFaultTest program stopped!Please check lampClose the lid.Error [xx]Test program stops when the instrument is started Check the lamp and replace it if necessary. Close lid. Press Start Again.FaultTest program stopped!Please remove the cuvetteClose the lid.Test program stops when the instrument is started Remove the cuvette/sample cell from the cell compartment.Press OK.ErrorSelfcheck stopped.Hardware error.Error [x]Electronic defectContact the manufacturer or a sales representative and indicate the error numberErrorToo much ambient light!Move instrument into shadeor close the lidThe instrument sensors detects too much ambient light.Decrease ambient light. (Avoid direct sunlight.) Close lid.No help function is available for this program.Shelf life exceeded! Use chemicals? The analysis is possibly erroneous. Use new chemicalsNo evaluation! Error in the test database / user databaseCheck programmingContact the manufacturer or a sales representativeNo barcode! No barcode foundReinsert the cell.If the barcode is not recognised, contact the manufacturer or his representative.No instrument backup present! Check the USB memory stick. No valid data for these parameters! Data analysis not possible, no measurement data Change the selection.No valid data found! View Data not possible in data log Change the selection.No help function present.No measurement data present! Data analysis settings cannot be configured without measurement data. Change the selection.Control range not reached! Data analysis limits not reached This is a warning notice. The control limit set was not reached. Control range exceeded! Data analysis limits exceeded. This is a warning notice. The control limit was exceeded.Concentration too high! Calculated concentration is higher than 999999 Dilute sample and measure againError displayed Definition Resolution
135TroubleshootingOver measuring range The measured absorbance is above the calibration range of the test Dilute sample and measure againUnder measuring range The measured absorbance is below the calibration range of the testIf possible, select a test with a lower reading range or use a cuvette with a longer path lengthPossible interference by: Interference Check The analysis is possibly erroneous due to interferences.Possible interference from: Interference Check The analysis is possibly erroneous due to interferences.Next service is due!Contact the manufacturer or his representative for an inspection of the instrument. For setting the service reminder, refer to Section 6.8.7, page 123Negative result! The calculated result is negative Check concentration of sampleNetwork switched off. Network setup off, when access to Viper homepage via sidebar Activate the online connection.Remote server cannot be reached. Error during network setup Make sure that the instrument is connected to the network. Unstable lighting conditions! Avoid direct sunlight at the measuring location.Insufficient memory for update . Error during update. Select a memory with more space.System check incorrect! Measurement of air values failedSwitch the instrument of and then back on again. If the system check is not successful, contact the manufacturer or his representative.Temperature too high.Measurement not possible!Switch of the instrument and allow it to cool for a few minutes. If necessary, move it to a cooler place.Update file is faulty. Error during update. Save the update file again and repeat the procedure.USB memory stick is not connected. Update not possible. Check the USB memory stick. Web server cannot be reached. Viper homepage cannot be reached. Try the connection again later.Error displayed Definition Resolution
136Troubleshooting
137Section 9 Replacement Parts9.1 AccessoriesDescription Cat. No.SIP 10 sipper set for DR 3900 with 1 inch round cell, EU LQV157.99.10001RFID read/write device LOC 100: Transponder for location RFID, EU LQV156.99.10001DR/check gel test standard 2763900Verification kit LZV537Thermal printer - Citizen PD 24, EU power cable 5835900.00Thermal printer - Citizen 24, UK power cable 5835900.82Thermal printer - Citizen 24, US power cable 296010Thermal printer, Citizen PD 24, CH power cable 5835900.00Paper for Citizen PD 24 LZM078Paper for Citizen PD 24, US 5836000Hand barcode scanner LZV566USB-Memory Stick LZV568Protective cap for USB port LZV881USB extension cord LZV567SD memory card LZV791Reader for SD/MMC memory card, USB connection LZY522Ethernet cable, shielded, 20 m in length. LZV873Interface cable USB - computer XLH926Cross-over cable LZX9981 inch rectangular cells, matched pair, glass (10 ml) 24954021 cm rectangular cells, matched pair, glass (3.5 ml) 20951001 cm rectangular cell, quartz 26244101 inch round cell, glass, with cap (10 ml) 21228001 cm single use rectangular micro-cell (1.5 ml) 26295001 cm rectangular plastic cells 27434005 cm rectangular cell, glass (17.5 ml) 26292505 cm rectangular cell, quartz (17.5 ml) 26244501 cm rectangular flow cell, quartz, (450 µl) LZV5105 cm rectangular flow cell, quartz (370 µl) LZV649Stoppers, neoprene for 1 inch rectangular cells 1480801
138Replacement Parts9.2 Replacement PartsDescription Cat. No.Halogen lamp LZV565Cell adapter A for 1-cm rectangular and 1-inch round cells LZV846Cell adapter B for 3-cm rectangular cells, China only LZV847Light shield LZV849Bench top power supply LZV844Power cord EU YAA080Power cord CH XLH051Power cord UK XLH057Power cord US XLH055Power cable China/Australia XLH069Dust Cover LZV845Cell compartment LZV848
139Section 10 Contact HACH CompanyWorld HeadquartersP.O. Box 389Loveland, Colorado80539-0389 U.S.A.Tel (800) 227-HACH(800) -227-4224(U.S.A. only)Fax (970) 669-2932orders@hach.comwww.hach.comRepair Service in theUnited States:HACH CompanyAmes Service100 Dayton AvenueAmes, Iowa 50010Tel (800) 227-4224(U.S.A. only)Fax (515) 232-3835Repair Service in Canada:Hach Sales & ServiceCanada Ltd.1313 Border Street, Unit 34Winnipeg, ManitobaR3H 0X4Tel (800) 665-7635(Canada only)Tel (204) 632-5598Fax (204) 694-5134canada@hach.comRepair Service inLatin America, the Caribbean, the Far East, Indian Subcontinent, Africa, Europe, or the Middle East:Hach Company WorldHeadquarters,P.O. Box 389Loveland, Colorado,80539-0389 U.S.A.Tel +001 (970) 669-3050Fax +001 (970) 669-2932intl@hach.comHACH LANGE GMBHWillstätterstraße 11D-40549 DüsseldorfTel. +49 (0)2 11 52 88-320Fax +49 (0)2 11 52 88-210info@hach-lange.dewww.hach-lange.deHACH LANGE LTDPacific WaySalfordGB-Manchester, M50 1DLTel. +44 (0)161 872 14 87Fax +44 (0)161 848 73 24info@hach-lange.co.ukwww.hach-lange.co.ukHACH LANGE LTDUnit 1, Chestnut RoadWestern Industrial EstateIRL-Dublin 12Tel. +353(0)1 460 2522Fax +353(0)1 450 9337info@hach-lange.iewww.hach-lange.ieHACH LANGE GMBHHütteldorfer Str. 299/Top 6A-1140 WienTel. +43 (0)1 912 16 92Fax +43 (0)1 912 16 92-99info@hach-lange.atwww.hach-lange.atHACH LANGE GMBHRorschacherstrasse 30aCH-9424 RheineckTel. +41 (0)848 55 66 99Fax +41 (0)71 886 91 66info@hach-lange.chwww.hach-lange.chHACH LANGE FRANCE S.A.S.8, mail Barthélémy ThimonnierLognesF-77437 Marne-La-Vallée cedex 2Tél. +33 (0) 820 20 14 14Fax +33 (0)1 69 67 34 99info@hach-lange.frwww.hach-lange.frHACH LANGE NV/SAMotstraat 54B-2800 MechelenTel. +32 (0)15 42 35 00Fax +32 (0)15 41 61 20info@hach-lange.bewww.hach-lange.beDR. LANGE NEDERLAND B.V.Laan van Westroijen 2aNL-4003 AZ TielTel. +31(0)344 63 11 30Fax +31(0)344 63 11 50info@hach-lange.nlwww.hach-lange.nlHACH LANGE APSÅkandevej 21DK-2700 BrønshøjTel. +45 36 77 29 11Fax +45 36 77 49 11info@hach-lange.dkwww.hach-lange.dkHACH LANGE ABVinthundsvägen 159ASE-128 62 SköndalTel. +46 (0)8 7 98 05 00Fax +46 (0)8 7 98 05 30info@hach-lange.sewww.hach-lange.seHACH LANGE S.R.L.Via Riccione, 14I-20156 MilanoTel. +39 02 39 23 14-1Fax +39 02 39 23 14-39info@hach-lange.itwww.hach-lange.itHACH LANGE S.L.U.Edif. Arteaga CentrumC/Larrauri, 1C- 2ª Pl.E-48160 Derio/VizcayaTel. +34 94 657 33 88Fax +34 94 657 33 97info@hach-lange.eswww.hach-lange.esHACH LANGE LDAAv. do Forte nº8Fracção MP-2790-072 CarnaxideTel. +351 214 253 420Fax +351 214 253 429info@hach-lange.ptwww.hach-lange.ptHACH LANGE SP. ZO.O.ul. Krakowska 119PL-50-428 WrocławTel. +48 801 022 442Fax +48 717 174 088info@hach-lange.plwww.hach-lange.plHACH LANGE S.R.O.Zastrčená 1278/8CZ-141 00 Praha 4 - ChodovTel. +420 272 12 45 45Fax +420 272 12 45 46info@hach-lange.czwww.hach-lange.czHACH LANGE S.R.O.Roľnícka 21SK-831 07 Bratislava – VajnoryTel. +421 (0)2 4820 9091Fax +421 (0)2 4820 9093info@hach-lange.skwww.hach-lange.skHACH LANGE KFT.Vöröskereszt utca. 8-10.H-1222 Budapest XXII. ker.Tel. +36 1 225 7783Fax +36 1 225 7784info@hach-lange.huwww.hach-lange.huHACH LANGE S.R.L.Str. Căminului nr. 3,et. 1, ap. 1, Sector 2RO-021741 BucureştiTel. +40 (0) 21 205 30 03Fax +40 (0) 21 205 30 17info@hach-lange.rowww.hach-lange.roHACH LANGE8, Kr. Sarafov str.BG-1164 SofiaTel. +359 (0)2 963 44 54Fax +359 (0)2 866 15 26info@hach-lange.bgwww.hach-lange.bgHACH LANGE SUANALİZ SİSTEMLERİ LTD.ŞTİ.Ilkbahar mah. Galip Erdem Cad. 616 Sok. No:9TR-Oran-Çankaya/ANKARATel. +90312 4908300 Ext. 140Fax +90312 4919903bilgi@hach-lange.com.trwww.hach-lange.com.tr
140ContactHACH LANGE D.O.O.Fajfarjeva 15SI-1230 DomžaleTel. +386 (0)59 051 000Fax +386 (0)59 051 010info@hach-lange.siwww.hach-lange.siΗΑCH LANGE E.Π.Ε.Αυλίδος 27GR-115 27 ΑθήναΤηλ. +30 210 7777038Fax +30 210 7777976info@hach-lange.grwww.hach-lange.grHACH LANGE D.O.O.Ivana Severa bbHR-42 000 VaraždinTel. +385 (0) 42 305 086Fax +385 (0) 42 305 087info@hach-lange.hrwww.hach-lange.hrHACH LANGE MAROC SARLAUVilla 14 – Rue 2 Casa PlaisanceQuartier Racine ExtensionMA-Casablanca 20000Tél. +212 (0)522 97 95 75Fax +212 (0)522 36 89 34info-maroc@hach-lange.comwww.hach-lange.ma
141Section 11 Limited warrantyHach Company warrants its products to the original purchaser against any defects that are due to faulty material or workmanship for a period of one year from date of shipment unless otherwise noted in the product manual.In the event that a defect is discovered during the warranty period, Hach Company agrees that, at its option, it will repair or replace the defective product or refund the purchase price excluding original shipping and handling charges. Any product repaired or replaced under this warranty will be warranted only for the remainder of the original product warranty period.This warranty does not apply to consumable products such as chemical reagents; or consumable components of a product, such as, but not limited to, lamps and tubing.Contact Hach Company or your distributor to initiate warranty support. Products may not be returned without authorization from Hach Company.LimitationsThis warranty does not cover:Damage caused by acts of God, natural disaster, labor unrest, acts of war (declared or undeclared), terrorism, civil strife or acts of any governmental jurisdictionDamage caused by misuse, neglect, accident or improper application or installation•Damage caused by any repair or attempted repair not authorized by Hach Company•Any product not used in accordance with the instructions furnished by Hach Company•Freight charges to return merchandise to Hach Company•Freight charges on expedited or express shipment of warranted parts or product•Travel fees associated with on-site warranty repairThis warranty contains the sole express warranty made by Hach Company in connection with its products. All implied warranties, including without limitation, the warranties of merchantability and fitness for a particular purpose, are expressly disclaimed.Some states within the United States do not allow the disclaimer of implied warranties and if this is true in your state the above limitation may not apply to you. This warranty gives you specific rights, and you may also have other rights that vary from state to state.This warranty constitutes the final, complete, and exclusive statement of warranty terms and no person is authorized to make any other warranties or representations on behalf of Hach Company. Limitation of RemediesThe remedies of repair, replacement or refund of purchase price as stated above are the exclusive remedies for the breach of this
142Limited warrantywarranty. On the basis of strict liability or under any other legal theory, in no event shall Hach Company be liable for any incidental or consequential damages of any kind for breach of warranty or negligence.
143Appendix AHelp GuideThe Help Guide is a step by step set of instructions for the DR 3900, for the execution of a stored program or procedure in accordance with process specifications. The Help Guide is available for the most used HACH LANGE tests and the Hach powder pillow reagent tests.A.1 Display of the Help Guide for stored programs1. Press Stored Programs in the Main Menu to view an alphabetical list of stored programs with program numbers. Highlight the required test and press Start.2. Press the information icon.3. The first procedure step according to the selected test/method will be displayed.Note: Use the arrow keys to navigate through the next or previous pages or to the first or last page.4. Follow the step-by-step descriptions on the screen.5. If the method requires a timer, press Cancel and then press the timer icon.
144Help GuideA list of preset times will be displayed.6. Select the necessary time.7. After the timer has finished, return to the Help Guide by pressing the information icon.8. The next help screen will be activated9. Press Cancel to return to the reading screen of the test/method.10. Press the information icon to return to the last current page of the Help Guide and proceed with the test.A.2 Display of the Help Guide for barcode programs1. Insert the light shield in cell compartment (2). 2. Press Barcode Programs in the Main Menu. Refer to Section 5.5, page 65 for more information.3. Press the information icon. 4. The list for barcodes will appear in increasing numerical order.5. Select the required test.6. Prepare the barcode test in accordance with current process specifications and follow the step by step instructions for the procedure.Note: Make sure that the values of the current process specification are used.Note: Use the arrow keys to navigate through the next or previous pages or to the first or last page.
145Help Guide7. Press Cancel to return to the measurement screen for the test.8. Press the information icon to return to the last current page of the Help Guide and complete the test.9. Insert the zero cell or sample cell (corresponding to the process specification) into the cell compartment. The reading is started automatically and the results are displayed.
146Help Guide
147IndexAAbsorbance Check ................................................. 116Absorbance Formula .............................................. 100Alphanumeric keypad ............................................... 25AQA ....................................................................... 117Audio signals ............................................................ 36BBarcode Programs ........................... 26, 65, 66, 67, 68editing ................................................................... 67Basic data .......................................................... 57, 66Benchtop power supply ............................................ 15CCalibration Settings .................................................. 78Cell adapter .............................................................. 19Cell compartment (1) ................................................ 18Cell compartment (2) ................................................ 18Cell compartment cover ........................................... 16Chemical Form ................................................... 58, 60Chemical form .................................................... 66, 67CleaningDisplay ................................................................ 127Spectrophotometer ............................................. 127Colour selection ....................................................... 43Complaints ............................................................. 141Concentration Factor ......................... 97, 98, 100, 101Concentration Resolution ................... 97, 98, 100, 102Control bar ............................................................... 65Cursor Mode .................................................. 103, 104Cuvettes/cells ......................................................... 128DData .......................................................................... 43deleting ..................................................... 43, 46, 48recalling .......................................................... 43, 48sending ........................................................... 43, 48storing ............................................................. 43, 48Data Folder .............................................................. 41Data Log ................................................................... 43Data log ...................................................................... 9Data Storage ............................................................ 48Date and time ........................................................... 35Default Gateway ...................................................... 40Dilution Factor .............................................. 58, 59, 67Display ....................................................... 25, 43, 127Display and Sound ................................................... 36Disposal ................................................................... 11Documentation overview ........................................ 126Drift Check ............................................................. 124EEditing Tests ............................................................. 63Ethernet communication .......................................... 39Ethernet port ............................................................ 18FFactory Service ...................................................... 123Favorites ...................................................... 26, 64, 92Favourites ................................................................ 91deleting ................................................................. 92editing ................................................................... 91recalling ................................................................ 92Filter Settings ........................................................... 44Formula .............................................................. 69, 70Free programming .................................. 84, 90, 91, 92HHelp Guide ............................................................. 143IInstrument Backup ................................................. 122Instrument Information ........................................... 111Instrument Setup ...... 26, 27, 58, 67, 98, 100, 103, 108Integral ........................................................... 103, 104Interfaces ........................................................... 18, 37Internet ............................................................. 72, 111IP address ................................................................ 39LLamp, refer to VIS lampLanguage ................................................................. 23Liability ................................................................... 141Light shield ............................................................... 21Link2SC .................................................................. 126local networks .......................................................... 39MMain menu ............................................................... 26Maintenance ........................................................... 127Manual Update ......................................................... 63Marking .................................................................. 110Measurement process .................................. 69, 70, 84Measuring sequence ................................................ 84Multi Wavelength ............ 26, 76, 78, 99, 100, 101, 102NName ........................................................................ 69
148IndexNetwork setup .......................................................... 39Noise Check ........................................................... 116OOperating mode ......................................................... 9Operator ID .............................................................. 27creating ................................................................. 27Optical Checks ............................... 112, 115, 116, 124Output Checks ....................................................... 124Overview of Product/Function .................................. 14PPassword ........................................................... 31, 43deactivate ............................................................. 34PC and Printer ................................................... 37, 38PC and printer .......................................................... 37Photometric accuracy ................................................ 9Photometric linearity .................................................. 9Photometric measuring range .................................... 9Printer Setup ............................................................ 37Printing data ............................................................. 38Program overview .................................................... 26Programming ..................................................... 72, 74RRatio ......................................................................... 53Reagent Blank ............................................. 58, 61, 67Recall Data ............... 49, 58, 67, 98, 99, 100, 103, 108Recall measurement data ........................................ 26Reference Scan ............................................. 103, 106Replaceable cell compartment ............................... 131RFID module ...................................................... 12, 16SSafety information .................................................... 11Sample blank ........................................................... 68Sample ID ................................................................ 29creating ................................................................. 29deleting ................................................................. 30Save as User Program ......................... 58, 67, 98, 100sc controller ............................................................ 126Scale & Units .................................. 103, 105, 108, 109Scanning speed ......................................................... 9Security List ............................................................. 31Select View ............................................................ 104Self-Check ............................................................... 23Send Data .................................... 45, 58, 67, 103, 108Server Name ............................................................ 41Service Time .......................................................... 123Single Wavelength ........................... 26, 75, 78, 97, 98Software ........................................................... 72, 111Source lamp ............................................................... 9Specifications ............................................................. 9Spectral bandwidth ..................................................... 9Standard Addition ............................................... 58, 93Standard Adjust .................................................. 58, 60Startup melody ......................................................... 23Startup process ........................................................ 23Stored Data .............................................................. 44Stored Programs ...... 26, 57, 58, 59, 60, 61, 63, 64, 67sending Data ......................................................... 58Stray light ................................................................... 9Stray Light Check ................................................... 116Subnet Mask ............................................................ 40System Check .................................................. 23, 123System Checks ........ 26, 111, 115, 116, 122, 123, 124TTime & Interval ............................................... 108, 109Time Course ......................................... 48, 49, 50, 108Time course ............................................. 26, 108, 110Time course scan reading ...................................... 109Timer ............................ 36, 58, 59, 67, 69, 71, 97, 100Toolbar ................................................................... 124Touch screen ............................................................ 25Troubleshooting ..................................................... 133UUnpack the instrument ............................................. 15Update ........................................................ 68, 69, 111Updating Tests ......................................................... 63USB port type A ....................................................... 16USB port type B ....................................................... 17User Programs ......................................................... 74User programs ............................................... 9, 26, 73VVariables ...................................................... 69, 70, 90Verification Kit ........................................................ 124Version ..................................................................... 69View Table .............................................................. 108Viper-Web .............................................................. 126VIS lamplamp controlVIS-LampLamp History ....................................................... 124
149IndexWWarranty ................................................................. 141Wavelength ...................................................... 97, 100Wavelength Accuracy ................................................ 9Wavelength calibration ............................................... 9Wavelength Check ................................................. 115Wavelength Range ................................................. 103Wavelength range ...................................................... 9Wavelength reproducibility ......................................... 9Wavelength resolution ................................................ 9Wavelength Scan ........... 26, 48, 49, 50, 102, 103, 106Wavelength selection ................................................. 9Wavelength setting ................................................. 108Wavelength spectrum ............................................ 103
150Index

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